CN216499451U

CN216499451U - Microfluidic droplet generation chip device capable of being connected with injector

Info

Publication number: CN216499451U
Application number: CN202123227085.1U
Authority: CN
Inventors: 尤佳霖; 庄添驰; 季明辉; 徐婷
Original assignee: Nanjing Medical University
Current assignee: Nanjing Medical University
Priority date: 2021-12-21
Filing date: 2021-12-21
Publication date: 2022-05-13
Anticipated expiration: 2031-12-21

Abstract

The utility model relates to a microfluidic droplet generation chip device capable of being connected with an injector, belonging to the technical field of microfluidics. After the pintle of the injector is taken down, the microfluidic droplet generation chip can be connected and fixed with the nipple of the injector through the connecting seat. The liquid drop generating cylinder of the micro-fluidic chip is embedded into the syringe of the injector, and the triangular liquid drop generating unit at the tail end of the liquid drop generating cylinder is soaked by the oil phase which is pumped in advance. Depending on its special shape, when the pumped solution enters the oil phase in the cylinder through the micro-channel and the droplet generation unit, the solution will generate static instability when the narrow channel suddenly enlarges and enters the oil phase, and then the solution shrinks to generate monodisperse droplets. The size of these droplets is determined only by the triangular shape of the droplet-generating unit, so that the microchannels and droplet-generating units can be arranged in series at high density, improving the throughput of droplet preparation while maintaining the uniformity and stability of the droplets. The generated liquid drops can be stored in the injector, so that the liquid drops are convenient to store and transfer.

Description

Microfluidic droplet generation chip device capable of being connected with injector

Technical Field

The utility model relates to a microfluidic droplet generation chip device capable of being connected with an injector, and belongs to the technical field of microfluidics.

Background

A Microfluidic chip (Microfluidic chip) is a scientific technology which is mainly characterized by the manipulation of fluids in a micron-scale space, and can realize various functions of conventional chemical or biological experiments through a Microfluidic network formed by microchannels.

Compared with the conventional experimental technology, the microfluidic technology has the characteristics of easiness in control, high reaction speed, small sample consumption, less pollution, low cost, easiness in integration and the like, can be used for simultaneously analyzing hundreds of samples in a few minutes or even shorter time, and can realize the whole processes of pretreatment and analysis of the samples on line.

The micro-fluidic chip has been developed from the initial micro total analysis system into a novel functional universal tool and platform applied to the fields of sample separation and analysis, materials, chemistry, biology, medicine and the like, and has wide application.

However, the use of the chip usually needs to be matched with a specific centrifugal microfluidic chip detection system comprising a mechanical control system and other systems, a mechanical motor provides power for the dispersion of the droplets, and uniform and stable water-in-oil droplets are further produced, but the detection efficiency and the throughput of the detection mode are greatly limited because the device can only use an amplification tube as a nucleic acid detection container and carrier. And because the chip needs to be matched with a power device, a storage pipe and other matched equipment for use, the system is difficult to transfer and transport, cross contamination can be caused in the operation processes of transferring liquid drops and the like, and the field detection requirements of pathogenic microorganisms and the like are difficult to meet.

SUMMERY OF THE UTILITY MODEL

The technical scheme provided by the utility model for solving the technical problems is as follows: a microfluidic droplet generation chip device which can be connected with a syringe.

The micro-fluidic droplet generation chip can be directly connected with a medical injector, and the micro-channel and the special droplet generation unit which are closely arranged are formed by a step emulsification droplet generation principle technology, so that the micro-fluidic droplet generation chip can be directly used for sucking a solution by the injector after being installed in the injector for injecting an oil phase without depending on an external device and a complex pipeline, thereby producing uniform and stable water-in-oil droplets, improving the flux, saving the space and the cost, facilitating the storage, the quantification and the transfer of the droplets and improving the field detection efficiency.

In order to solve the technical problems, the utility model is realized by the following technical scheme: a microfluidic droplet generation chip device capable of being connected with an injector comprises the injector and a microfluidic droplet generation chip capable of being connected with the injector; the injector comprises a nipple 7, a needle cylinder 8 and a piston 9; the micro-fluidic liquid drop generating chip comprises a suction port 1, a liquid drop generating cylinder 4, a connecting seat 2 and an auxiliary plate 3; the suction port 1 is positioned at the upper part of the microfluidic droplet generation chip, the auxiliary plate 3 is positioned at the lower part of the microfluidic droplet generation chip, the connecting seat 2 is connected with the suction port 1 and the auxiliary plate 3, and the droplet generation cylinder 4 is arranged in the connecting seat 2 and is connected with the suction port 1;

the liquid drop generating cylinder 4 is in a sieve shape and comprises a micro-channel 5 and a liquid drop generating unit 6, the micro-channel 5 is a cuboid, the number of the micro-channel 5 is dozens to hundreds, and the micro-channel is arranged in the liquid drop generating cylinder 4 in a high-density parallel mode; the liquid drop generating unit 6 is connected with the micro-channel 5 at the tail end of the micro-channel 5, the shape of the liquid drop generating unit is triangular prism, the height of the side surface of the triangular prism is h, the length of the triangular prism is w, the ratio of w to h is 5.5-19: 1;

the micro-fluidic droplet generation chip replaces the position of the original pintle of the injector and is connected with the nipple 7 of the injector through the connecting seat 2;

the droplet generation cylinder 4 is arranged in the needle cylinder after the microfluidic droplet generation chip is connected with the injector.

Preferably, the syringe may be 1mL, 2mL, 5mL, 10mL or 50mL of a syringe which is generally used clinically.

Preferably, the aspiration port 1 is used to aspirate a solution into the droplet generation cartridge 4 of the microfluidic droplet generation chip.

Preferably, the chip auxiliary plate 3 is used for fixing or detaching the microfluidic droplet generation chip to or from the syringe.

A microfluidic droplet generation chip device capable of being connected with a syringe has the following principle:

the device comprises a syringe and a microfluidic droplet generation chip connectable to the syringe. After the pintle of the injector is taken down, the microfluidic droplet generation chip can be connected and fixed with the nipple of the injector through the connecting seat. The liquid drop generating cylinder of the microfluidic chip is embedded in the needle cylinder of the injector, and the triangular liquid drop generating unit at the tail end of the liquid drop generating cylinder is soaked by the oil phase which is pumped in advance. Depending on its special shape, when the pumped solution enters the oil phase in the cylinder through the micro-channel and the droplet generation unit, the solution will generate static instability when the narrow channel suddenly enlarges and enters the oil phase, and then the solution shrinks to generate monodisperse droplets. The size of these droplets is determined only by the triangular shape of the droplet-generating unit, so that the microchannels and droplet-generating units can be arranged in series at high density, improving the throughput of droplet preparation while maintaining the uniformity and stability of the droplets. The generated liquid drops can be stored in the injector, and are convenient to store and transfer.

Compared with the prior art, the utility model has the beneficial effects that:

1. the microfluidic droplet generation chip is matched with a commonly used medical injector, firstly, in the clinical nucleic acid detection process, monodisperse droplets can be conveniently and rapidly produced on site without depending on external equipment and complex pipelines, the operation is simple, the installation is convenient, and the production cost is reduced. Secondly, the liquid drops do not need to be additionally transferred to other containers, the liquid drops which can be generated in one step can be directly stored and quantified in the needle cylinder, the pollution chance is reduced, and the safety is guaranteed.

2. By adopting a step emulsification technology and a high-density arrangement mode of micro-channels and droplet generation units, the chip space is fully utilized, and the purposes of improving droplet generation flux, ensuring uniform droplet size and stable properties are achieved.

Drawings

The utility model will be further explained with reference to the drawings.

Fig. 1 is an external schematic view of a microfluidic droplet generation chip device to which a syringe may be attached.

FIG. 2 is an external view of a droplet generation chip and an injector.

FIG. 3 is a cross-sectional view of a microfluidic droplet generation chip device to which a syringe may be attached.

Fig. 4 is an external schematic view of a droplet generation chip.

Fig. 5 is a schematic diagram of a droplet generation chip.

Wherein: 1-a suction port; 2-a connecting seat; 3-an auxiliary plate; 4-a droplet generation cartridge; 5-a microchannel; 6-a droplet generation unit; 7-nipple; 8-needle cylinder; 9-piston.

Detailed Description

Example 1

A microfluidic droplet generation chip device capable of being connected with an injector comprises the injector and a microfluidic droplet generation chip capable of being connected with the injector; the injector comprises a nipple 7, a needle cylinder 8 and a piston 9; the micro-fluidic liquid drop generating chip comprises a suction port 1, a liquid drop generating cylinder 4, a connecting seat 2 and an auxiliary plate 3; the liquid drop generating cylinder 4 is in a sieve shape and comprises a micro-channel 5 and a liquid drop generating unit 6, the micro-channel 5 is a cuboid, the number of the micro-channel 5 is dozens to hundreds, and the micro-channel is arranged in the liquid drop generating cylinder 4 in a high-density parallel mode; the liquid drop generating unit 6 is connected with the micro-channel 5 at the tail end of the micro-channel 5, the shape of the liquid drop generating unit is triangular prism, the height of the side surface of the triangular prism is h, the length of the triangular prism is w, the ratio of w to h is 5.5-19: 1;

the suction port 1 is positioned at the upper part of the microfluidic droplet generation chip, the auxiliary plate 3 is positioned at the lower part of the microfluidic droplet generation chip, the droplet generation cylinder 4 is arranged in the connecting seat 2 and is connected with the suction port 1, and the auxiliary plate 3 is connected with the connecting seat 2;

The syringe may be 1mL, 2mL, 5mL, 10mL or 50mL of a syringe which is generally used clinically.

The suction port 1 is used to suck the solution into the droplet-generating cartridge 4 of the microfluidic droplet-generating chip.

The chip auxiliary plate 3 is used for fixing or detaching the chip to or from the syringe.

The syringe must be pre-pumped with a volume of oil phase liquid.

The oil phase is mineral oil.

8% mass fraction of surfactant is added to the oil phase to increase the stability of the stable droplets.

Firstly, the syringe piston is drawn to pump oil with enough volume into the syringe, then the pintle is replaced and taken down, the microfluidic droplet generation chip of the embodiment is replaced, and the connecting seat 2 of the chip is fixed on the syringe nipple 7. At this time, the droplet generation cartridge of the chip is embedded in the oil phase. The syringe piston is drawn to draw the desired solution from the suction head into the chip interior space, the liquid is drawn into the drop generating cartridge under suction, passes through the microchannels arranged in series, and monodisperse drops are generated at the interface of the drop generating unit and the oil due to static instability. These monodisperse droplets were stored in a syringe. Then the chip can be taken down and put on the pintle again to be sealed and stored. These droplets may be pushed out for use by a syringe.

The present invention is not limited to the above-described embodiments. All technical solutions formed by equivalent substitutions fall within the protection scope of the claims of the present invention.

Claims

1. A microfluidic droplet generation chip device connectable to a syringe, comprising:

comprises an injector and a microfluidic droplet generation chip which can be connected with the injector; the injector comprises a nipple (7), a needle cylinder (8) and a piston (9); the microfluidic droplet generation chip comprises an attraction port (1), a droplet generation cylinder (4), a connecting seat (2) and an auxiliary plate (3); the suction port (1) is positioned at the upper part of the microfluidic droplet generation chip, the auxiliary plate (3) is positioned at the lower part of the microfluidic droplet generation chip, the connecting seat (2) is connected with the suction port (1) and the auxiliary plate (3), and the droplet generation cylinder (4) is arranged in the connecting seat (2) and is connected with the suction port (1); the liquid drop generating cylinder (4) is in a sieve shape and comprises a micro-channel (5) and a liquid drop generating unit (6), the micro-channel (5) is a cuboid, the number of the micro-channel is dozens to hundreds, and the micro-channel is arranged in the liquid drop generating cylinder (4) in parallel at high density; the liquid drop generating unit (6) is connected with the micro-channel (5) at the tail end of the micro-channel (5), the shape of the liquid drop generating unit is triangular prism, the height of the side surface of the triangular prism is h, the length of the triangular prism is w, the ratio of w to h is 5.5-19: 1;

the micro-fluidic liquid drop generating chip replaces the position of the original pintle of the injector and is connected with the nipple (7) of the injector through the connecting seat (2);

the liquid drop generating cylinder (4) is arranged in the needle cylinder after the microfluidic liquid drop generating chip is connected with the injector.

2. The microfluidic droplet generation chip device connectable to a syringe according to claim 1, wherein: the syringe may be 1mL, 2mL, 5mL, 10mL or 50mL of a syringe which is generally used clinically.

3. The microfluidic droplet generation chip device connectable to a syringe according to claim 1, wherein: the suction port (1) is used for sucking the solution into a liquid drop generating cylinder (4) of the microfluidic liquid drop generating chip.

4. The microfluidic droplet generation chip device connectable to a syringe according to claim 1, wherein: the chip auxiliary plate (3) is used for fixing or detaching the microfluidic droplet generation chip to or from the injector.