CN216484802U

CN216484802U - Double-column chromatography online detection system

Info

Publication number: CN216484802U
Application number: CN202121323938.9U
Authority: CN
Inventors: 于晓军
Original assignee: Nanjing Baisai Biochromatography Technology Co ltd
Current assignee: Nanjing Baisai Biochromatography Technology Co ltd
Priority date: 2021-06-15
Filing date: 2021-06-15
Publication date: 2022-05-10
Anticipated expiration: 2031-06-15

Abstract

The utility model discloses a double-column chromatography online detection system, which comprises chromatography columns which are arranged in bilateral symmetry, wherein sample loading pumps are fixedly installed at the tops of the two chromatography columns, a filler is filled in the middle of each chromatography column, the bottoms of the chromatography columns on the left side and the right side are respectively and fixedly connected with a first switching valve and a second switching valve, the first switching valve and the second switching valve are fixedly connected with a third switching valve through a three-head connecting pipe, the output end of the third switching valve is fixedly connected with a detection pump, the output end of the detection pump is fixedly connected with a detector, and the detector and the three-head connecting pipe are also combined and connected with a sample collecting port, and the double-column chromatography online detection system has the beneficial effects that: during large-scale chromatography, a small-flow detector is used for on-line detection at the same time, so that the cost is low and the efficiency is high.

Description

Double-column chromatography online detection system

Technical Field

The utility model relates to the technical field of chromatographic separation, in particular to a double-column chromatography online detection system.

Background

Chromatography is a short term chromatography, and a method for separating and determining a multi-component mixture by utilizing factors with different physical properties of various components is widely applied to the fields of pharmacy, fine chemical engineering and the like; the method comprises adsorption chromatography and separation chromatography, and is generally used for analysis of organic compounds, metal ions and amino acids; chromatography utilizes different distribution ratios of substances between a stationary phase and a mobile phase to achieve the separation purpose.

The column chromatography is also called chromatography or chromatography, the separation principle is to separate substances according to different adsorption capacities of the substances on a stationary phase, generally, substances with large polarity are easy to be adsorbed by the stationary phase, substances with small polarity are not easy to be adsorbed by the stationary phase, the column chromatography process is the processes of adsorption, desorption, re-adsorption and re-desorption, and the separation of silica gel as a fixed relatively complex organic compound in the column chromatography has higher efficiency, therefore, the silica gel is mainly used as a stationary phase in the biological oil column chromatographic separation, and compared with other separation methods, the column chromatography has the advantages of low energy consumption, easy recycling of eluent, high purity of obtained products and the like, but also has the defects of complex separation process, long time consumption and large amount of required solvent, in actual production, industrialization is difficult, so that simplification of a separation process, shortening of separation time and improvement of solvent utilization rate become key points for improvement of column chromatography separation methods in the future.

However, existing detectors tend to be suitable for small flows (tens to hundreds of milliliters per hour), and high flow detectors are expensive and of a small variety.

SUMMERY OF THE UTILITY MODEL

The present invention is directed to a dual-column chromatography online detection system, which solves the above problems in the prior art.

In order to achieve the purpose, the utility model provides the following technical scheme: the utility model provides a double-column chromatography on-line measuring system, includes the chromatography column that bilateral symmetry set up, two the top fixed mounting of chromatography column has the pump of getting ready, it has the filler to fill in the middle of the chromatography column, and the bottom of the chromatography column of the left and right sides is first diverter valve of fixed connection and second diverter valve respectively, first diverter valve and second diverter valve are through three takeovers fixed connection third diverter valve, the output fixed connection detection pump of third diverter valve, the output fixed connection detector of detection pump, detector and three takeovers still merge the connection sample collection mouth.

As a further scheme of the utility model: the fillers are layered from top to bottom, and the number of the layers of the fillers is not less than three.

As a further scheme of the utility model: the detector is specifically a UV detector, and the flow is less than 50 mL/min.

As a further scheme of the utility model: the inner diameter of the chromatographic column is 200-600mm, and the inner diameter of the chromatographic column is-times of the inner diameter of the connecting pipeline.

As a further scheme of the utility model: the detector also has signal output connected to the computer.

Compared with the prior art, the utility model has the beneficial effects that:

1. the sample to be separated and purified is pumped into the chromatographic column through the pump, the sample is separated through the filler, and the effluent sample flows through the detector, so that the detection result is more accurate;

2. during large-scale chromatography, a small-flow detector is used for carrying out online detection at the same time, so that the cost is low and the efficiency is high;

3. the desired fraction of the isolate may be collected by a detector.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a schematic diagram of the principles of the present invention;

FIG. 2 is a block diagram of the present invention.

In the figure: the device comprises a chromatographic column 1, a sample loading pump 2, a filler 3, a first switching valve 4, a second switching valve 5, a third switching valve 6, a detection pump 7, a detector 8 and a sample collection port 9.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-2, in an embodiment of the present invention, a dual-column chromatography online detection system includes chromatography columns 1 symmetrically arranged left and right, sample pumps 2 are fixedly installed on tops of the two chromatography columns 1, a filler 3 is filled in a middle of the chromatography columns 1, bottoms of the chromatography columns 1 on left and right sides are respectively and fixedly connected to a first switching valve 4 and a second switching valve 5, the first switching valve 4 and the second switching valve 5 are fixedly connected to a third switching valve 6 through a three-head connection pipe, an output end of the third switching valve 6 is fixedly connected to a detection pump 7, an output end of the detection pump 7 is fixedly connected to a detector 8, and the detector 8 and the three-head connection pipe are also connected to a sample collection port 9 in a combined manner.

It is noted that the fillers 3 are layered from top to bottom, and the number of layers of the fillers 3 is not less than three.

Notably, the detector 8 is embodied as a UV detector, and the flow rate is less than 50 mL/min.

It is noted that the inner diameter of the chromatography column 1 is 200-600mm, which is 5-8 times the inner diameter of the connecting tube.

It should be noted that the detector 8 is also connected to a computer for signal output.

The working principle is as follows:

firstly, preparing a sample, then opening a first switching valve 4 and a third switching valve 6, closing a second switching valve 5, starting a detection pump 7, finally opening a detector 8, then opening a sample loading pump to detect the sample in the first chromatographic column 1, and starting to collect the sample from a sample collection port 9 until the detection value changes after detection value data appears during detection;

similarly, the sample is prepared, the first switching valve 4 is closed, the second switching valve 5 and the third switching valve 6 are opened, the detection pump 7 is started, the detector 8 is finally opened, then the sample loading pump is opened to detect the sample in the second chromatographic column 1, and the sample collection from the sample collection port 9 is started until the detection value changes after the detection value data appears during detection.

It will be evident to those skilled in the art that the utility model is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the utility model being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims

1. The utility model provides a double column chromatography on-line measuring system, includes chromatographic column (1) that bilateral symmetry set up, its characterized in that: two top fixed mounting of chromatography column (1) has sample loading pump (2), it has filler (3) to fill in the middle of chromatography column (1), the bottom of the chromatography column (1) of the left and right sides is first diverter valve (4) of fixed connection and second diverter valve (5) respectively, first diverter valve (4) and second diverter valve (5) are through three takeovers fixed connection third diverter valve (6), the output fixed connection detection pump (7) of third diverter valve (6), the output fixed connection detector (8) of detection pump (7), detector (8) and three takeovers still merge and connect sample collection mouth (9).

2. The on-line detection system of double-column chromatography of claim 1, characterized in that: the fillers (3) are layered from top to bottom, and the number of layers of the fillers (3) is not less than three.

3. The on-line detection system of double-column chromatography of claim 1, characterized in that: the detector (8) is in particular a UV detector.

4. The on-line detection system of double-column chromatography of claim 1, characterized in that: the inner diameter of the chromatographic column (1) is 200-600mm, and the inner diameter is 5-8 times of the inner diameter of the connecting pipeline.

5. The on-line detection system of double-column chromatography of claim 1, characterized in that: the detector (8) is also connected with a computer through signal output.