CN216469357U

CN216469357U - Antibody incubation recovery box for WesternBlot experiment

Info

Publication number: CN216469357U
Application number: CN202122758707.7U
Authority: CN
Inventors: 刘振振; 陆思懿; 刘镇涛; 周鑫; 付卫
Original assignee: Peking University Third Hospital Peking University Third Clinical Medical College
Current assignee: Peking University Third Hospital Peking University Third Clinical Medical College
Priority date: 2021-11-11
Filing date: 2021-11-11
Publication date: 2022-05-10
Anticipated expiration: 2031-11-11

Abstract

The utility model discloses an antibody incubation recovery box for Western Blot experiments, and particularly relates to the field of Western Blot experiments. According to the Western Blot experiment device, the groove is formed in the front wall plate of the box body and is continuous with the box body bottom plate, the box body bottom plate is inclined by 45 degrees, the box body bottom plate is changed from a horizontal state to an inclined state, liquid on the box body bottom plate flows to the funnel cone of the groove, the external recovery tube is placed at the funnel hole tube, antibodies for the Western Blot experiment can be recovered conveniently, time and labor are saved, the cost is reduced, and the Western Blot experiment operation is facilitated.

Description

Antibody incubation recovery box for WesternBlot experiment

Technical Field

The utility model relates to the field of Western Blot experiments, in particular to an antibody incubation recovery box for Western Blot experiments.

Background

Western Blot (immunoblot assay) is used. It is a commonly used experimental method in molecular biology, biochemistry and immunogenetics. The basic principle is that the total proteins of cells or tissues are transferred from the gel to an NC membrane (nitrocellulose membrane) or PVDF (polyvinylidene fluoride membrane) membrane which is a solid support through electrophoresis, and then specific antibodies are used for detecting a specific antigen. The specific antigen detected in this technique refers to a protein of interest. Specific antibodies refer to primary and secondary antibodies (secondary antibody refers to an antibody to a primary antibody, e.g., if the primary antibody is obtained from a rabbit, the secondary antibody is an anti-rabbit IgG antibody, and both can specifically bind). The "primary antibody" was used as a "probe" and the labeled secondary antibody was used to "develop color". Information on the expression of a specific protein in the analyzed cell or tissue is obtained by analyzing the location and depth of staining.

In the prior art, still there are many disadvantages, for example, in the Western Blot experiment process, the PVDF membrane (polyvinylidene fluoride membrane) after completing membrane conversion and the antibody need to be incubated in the antibody incubation box, because the antibody (primary antibody) is more precious, the incubated primary antibody is usually recovered, the recovery operation is usually performed by using a pipette or a disposable pipette, which wastes time and labor, increases the cost, and the currently commonly used antibody incubation box does not have a convenient recovery function.

SUMMERY OF THE UTILITY MODEL

Technical problem to be solved

In order to overcome the defects in the prior art, the embodiment of the utility model provides an antibody incubation recovery box for Western Blot experiments, wherein a groove is formed in the front wall plate of a box body, the groove is continuous with a box body bottom plate, the box body bottom plate is inclined by 45 degrees, so that the box body bottom plate is changed from a horizontal state to an inclined state, liquid on the box body bottom plate flows to a funnel cone of the groove, an external recovery pipe is placed at a funnel hole pipe, the antibody for Western Blot experiments is convenient to recover, time and labor are saved, the cost is reduced, the operation of Western Blot experiments is convenient, and the problems in the background art are solved.

(II) technical scheme

In order to achieve the purpose, the utility model provides the following technical scheme: the utility model provides a box is retrieved with antibody incubation for Western Blot experiment, includes the box body bottom plate, the top both sides of box body bottom plate all are provided with the curb plate, the quantity of curb plate is two, the one end top of box body bottom plate is provided with preceding wallboard to and the other end top of box body bottom plate is provided with back wallboard, the top of back wallboard is provided with the top cap, the bottom surface of preceding wallboard is provided with the recess, the quantity of recess is a plurality of, each the inside of recess all is provided with the funnel circular cone, funnel circular cone and preceding wallboard fixed connection.

The specific implementation mode is as follows: be equipped with the recess through the antetheca at the box body, this recess continues with the box body bottom plate mutually, takes place 45 degrees slopes with the box body bottom plate for the box body bottom plate becomes the tilt state by the horizontality, lets the funnel circular cone department of the liquid stream to recess above the box body bottom plate, puts external recovery tube in funnel hole pipe department, is convenient for retrieve the antibody of Western Blot experiment usefulness, labour saving and time saving reduces the cost, makes things convenient for Western Blot experiment operation.

In a preferred embodiment, the front wall plate surface is provided with a groove which gradually widens from bottom to top.

In a preferred embodiment, one end of the groove extends to the top of the box body bottom plate and is arranged in an inclined mode, a funnel cone is arranged at the top end of the groove, a funnel hole pipe is arranged at the other end of the funnel cone, and the funnel hole pipe is communicated with the joint of the funnel cone.

In a preferred embodiment, the top of the bottom plate of the box body is provided with 3 separating plates, and 4 separating grooves with equal intervals are formed.

In a preferred embodiment, the partition plate is fixedly connected with the bottom plate of the box body.

In a preferred embodiment, the thickness (4.5mm) of the front wall plate is greater than the thickness (1.5mm) of the side plates and the rear wall plate, a rubber plug is arranged inside the funnel hole tube, and the rubber plug is fixedly connected with the front wall plate through a nylon rope.

(III) advantageous effects

The utility model provides an antibody incubation recovery box for Western Blot experiments, which has the following beneficial effects:

according to the Western Blot experiment device, the groove is formed in the front wall plate of the box body and is continuous with the box body bottom plate, the box body bottom plate is inclined by 45 degrees, the box body bottom plate is changed from a horizontal state to an inclined state, liquid on the box body bottom plate flows to the funnel cone of the groove, the external recovery tube is placed at the funnel hole tube, antibodies for the Western Blot experiment can be recovered conveniently, time and labor are saved, the cost is reduced, and the operation of the Western Blot experiment is facilitated.

Drawings

Fig. 1 is a top view of the overall structure of the present invention.

Fig. 2 is a front view of the front wall panel of the present invention.

The reference signs are: 1 box body bottom plate, 2 top covers, 3 recesses, 4 funnel cones, 5 funnel hole pipes, 6 division boards, 7 side boards, 8 front wall boards, 9 rear wall boards, 10 rubber plugs and 11 division grooves.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The utility model provides an antibody incubation recovery box for Western Blot experiments, which comprises a box body bottom plate 1, wherein two side plates 7 are arranged on two sides of the top end of the box body bottom plate 1, a front wall plate 8 is arranged at the top of one end of the box body bottom plate 1, a rear wall plate 9 is arranged at the top of the other end of the box body bottom plate 1, a top cover 2 is arranged at the top of the rear wall plate 9, grooves 3 are formed in the bottom end surface of the front wall plate 8, a plurality of grooves 3 are formed, a funnel cone 4 is arranged inside each groove 3, and the funnel cones 4 are fixedly connected with the front wall plate 8.

Furthermore, the surface of the front wall plate 8 is provided with a groove 3 which is gradually widened from bottom to top.

Further, the one end of recess 3 extends to the top of box body bottom plate 1, and sets up for the slope, the top of recess 3 is equipped with funnel circular cone 4, the center department of funnel circular cone 4 is equipped with funnel hole pipe 5, funnel hole pipe 5 is linked together with the junction of funnel circular cone 4, can let liquid pass through funnel hole pipe 5 and be convenient for retrieve liquid.

Furthermore, division plates 6 are arranged at the top of the box body bottom plate 1, the number of the division plates 6 is 3, 4 equally-spaced division grooves 11 are formed, and incubation of different antibodies can be completed conveniently.

Furthermore, the partition plate 6 is fixedly connected with the box body bottom plate 1.

Further, the thickness (4.5mm) of preceding wallboard 8 is greater than the thickness (1.5mm) of curb plate 7 and back wallboard 9, the inside of funnel orifice pipe 5 is provided with plug 10, plug 10 is through nylon rope and 8 fixed connection of preceding wallboard, and the prevention and cure plug is lost.

In conclusion, the working process of the utility model is as follows: when using, in the box is hatched to the antibody, pour the diluted antibody of experiment usefulness into, hatch, after hatching, carry out the antibody and retrieve, put external recovery tube in funnel hole pipe 5 department, take place the slope of 45 degrees with box body bottom plate 1, make box body bottom plate 1 become the tilt state by the horizontality, the antibody passes through in recess 3 gets into funnel hole pipe 5 gradually, discharge through funnel hole pipe 5, alright with retrieve the antibody, time saving and labor saving, reduce the cost, make things convenient for Western Blot experiment operation, prevent the condition of emergence weeping through plug 10 and funnel hole pipe 5.

The working principle of the utility model is as follows:

referring to the attached drawings 1-2 of the specification, the convenient antibody incubation recovery box for Western Blot experiments in the embodiment of the utility model is characterized in that diluted antibodies for experiments are poured into the antibody incubation box for incubation, after incubation is finished, the antibodies are recovered, an external recovery tube is placed at a funnel hole tube 5, the bottom plate 1 of the box body is inclined by 45 degrees, so that the bottom plate 1 of the box body is changed into an inclined state from a horizontal state, the antibodies gradually enter the funnel hole tube 5 through a groove 3, the antibodies for Western Blot experiments are convenient to recover, time and labor are saved, the cost is reduced, and the operation of the Western Blot experiments is convenient.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a box is retrieved with antibody incubation for Western Blot experiment, includes box body bottom plate (1), its characterized in that: the utility model discloses a storage box, including box body bottom plate (1), the top both sides of box body bottom plate (1) all are provided with curb plate (7), the quantity of curb plate (7) is two, the one end top of box body bottom plate (1) is provided with preceding wallboard (8) to and the other end top of box body bottom plate (1) is provided with back wallboard (9), the top of back wallboard (9) is provided with top cap (2), the bottom surface of preceding wallboard (8) is provided with recess (3), the quantity of recess (3) is a plurality of, each the inside of recess (3) all is provided with funnel circular cone (4), funnel circular cone (4) and preceding wallboard (8) fixed connection.

2. The Western Blot experiment antibody incubation recovery kit according to claim 1, wherein: the surface of the front wall plate (8) is provided with a groove (3) which is gradually widened from bottom to top.

3. The Western Blot experiment antibody incubation recovery kit according to claim 1, wherein: the one end of recess (3) extends to the top of box body bottom plate (1), and sets up for the slope, the top of recess (3) is equipped with funnel circular cone (4), the center department of funnel circular cone (4) is provided with funnel hole pipe (5), the junction of funnel hole pipe (5) and funnel circular cone (4) is linked together.

4. The Western Blot experiment antibody incubation recovery kit according to claim 1, wherein: the top of the box body bottom plate (1) is provided with partition plates (6), the number of the partition plates (6) is 3, and 4 partition grooves (11) with equal intervals are formed.

5. The Western Blot experiment antibody incubation recovery kit according to claim 4, wherein: the partition plate (6) is fixedly connected with the box body bottom plate (1).

6. The Western Blot experiment antibody incubation recovery kit according to claim 3, wherein the Western Blot experiment antibody incubation recovery kit comprises: thickness (4.5mm) of preceding wallboard (8) are greater than thickness (1.5mm) of curb plate (7) and back wallboard (9), the inside of funnel hole pipe (5) is provided with plug (10), the plug passes through nylon rope and preceding wallboard fixed connection.