PCR pipe capping device
Technical Field
The utility model relates to a PCR pipe capping device belongs to PCR processing technology field.
Background
At present, after a reaction system and an amplification template are added to a PCR tube for nucleic acid detection, a sealing cover of the PCR tube needs to be pressed or sealed by hands in sequence, and dozens of PCR tubes can be placed on the same placing plate.
SUMMERY OF THE UTILITY MODEL
Not enough to prior art exists, the utility model aims at providing a PCR pipe capping device to the prior art of solution is because the PCR pipe is less, presses the in-process of sealing tightly at frequent manual, and numb phenomenon appears in the hand easily, and then leads to objective application of force not enough easily, appears the closing cap or seals the membrane and does not compress tightly completely, leads to the volatile problem of amplification in-process reaction system easily.
In order to achieve the above purpose, the present invention is realized by the following technical solution: a PCR tube press cap apparatus comprising: the PCR tube plate comprises a rack, a gland mechanism, a driving assembly and a PCR tube plate, wherein the gland mechanism is fixedly arranged in the rack, the driving assembly is arranged above the gland mechanism, the driving assembly is fixedly arranged above the inside of the rack, and the PCR tube plate is arranged below the rack in a sliding manner;
the gland mechanism comprises a pressing plate, a telescopic rod, a guide pillar and a limiting sleeve, wherein the guide pillar is fixedly arranged on the rack, the limiting sleeve is slidably mounted on the guide pillar, the limiting sleeve penetrates through the pressing plate, the pressing plate is sleeved on the guide pillar, the telescopic rod is fixedly mounted in the middle of the upper portion of the pressing plate, and the upper portion of the telescopic rod is embedded in the driving assembly.
Furthermore, a plurality of pipe grooves are formed in the PCR pipe disc, branch guide pieces are attached to the upper surface of the PCR pipe disc, the sizes and the positions of the groove holes in the branch guide pieces correspond to those of the pipe grooves, and arc-shaped grooves are formed in the edges of the left side and the right side of each branch guide piece of the PCR pipe disc.
Furthermore, the driving assembly is a linear driver and consists of a motor push rod and a controller, and the motor push rod is fixedly connected with the telescopic rod.
Furthermore, the controller is provided with an ECU and a Hall sensor and is used for monitoring the rotating speed of the motor at any time.
Further, the rack comprises an upper top plate, a bottom plate and side plates, the side plates are arranged on the same side face of the upper top plate and the bottom plate, and the lower portion of the upper top plate is fixedly connected with the driving assembly.
Furthermore, two guide strips are arranged above the bottom plate in parallel, and a PCR tube tray is arranged above the bottom plate between the two guide strips in a sliding manner.
Further, the surface of the area of the bottom plate between the two conducting bars is smooth.
Furthermore, the stop collar comprises two hollow cylinders, and the diameter of the cylinder below is greater than that of the cylinder above.
Furthermore, the PCR tube plate is made of metal materials and has stronger rigidity and supporting force.
Advantageous effects
The utility model relates to a PCR pipe gland device, be equipped with gland mechanism structurally, can place on PCR pipe dish after through the PCR pipe closing cap or the pad pasting that will load reaction system and amplification template, through inserting gland mechanism below with PCR pipe dish, make the clamp plate push down perpendicularly through drive assembly drive telescopic link, can be with a plurality of PCR pipe closing cap steady the pipe of impressing that is loaded reaction system and amplification template or with a whole membrane of sealing, seal PCR pipe, exert pressure through regionality, the not totally enclosed state of being enough of objective application of force that appears when can effectively avoiding manual pipe pressing, avoid volatilizing of amplification in-process reaction system.
Drawings
Other features, objects and advantages of the invention will become more apparent upon reading of the detailed description of non-limiting embodiments with reference to the following drawings:
FIG. 1 is a schematic structural diagram of a PCR tube capping device according to the present invention;
FIG. 2 is a schematic diagram of the internal structure of a PCR tube capping device according to the present invention;
FIG. 3 is a detailed structural diagram of the capping mechanism;
FIG. 4 is a schematic top view of a PCR tube tray.
In the figure: the PCR reaction device comprises a frame-1, a gland mechanism-2, a driving assembly-3, a PCR tube disc-4 and a shell-5;
a pressing plate-21, a telescopic rod-22, a guide post-23 and a limiting sleeve-24;
a guide vane-41;
an upper top plate-11, a bottom plate-12 and a side plate-13;
conducting bar-121.
Detailed Description
In order to make the technical means, creation features, achievement purposes and functions of the present invention easy to understand, the present invention is further described below with reference to the following embodiments.
Referring to fig. 1-4, the present invention provides a PCR tube capping device, which comprises: the method comprises the following steps: the PCR tube plate comprises a rack 1, a gland mechanism 2, a driving component 3 and a PCR tube plate 4, wherein the gland mechanism 2 is fixedly arranged in the rack 1, the driving component 3 is arranged above the gland mechanism 2, the driving component 3 is fixedly arranged above the inside of the rack 1, and the PCR tube plate 4 is arranged below the rack 1 in a sliding manner; the capping mechanism 2 comprises a pressing plate 21, a telescopic rod 22, a guide post 23 and a limiting sleeve 24, the guide post 23 is fixedly arranged on the rack 1, the limiting sleeve 24 is slidably arranged on the guide post 23, the limiting sleeve 24 penetrates through the pressing plate 21, the pressing plate 21 is sleeved on the guide post 23, the telescopic rod 22 is fixedly arranged in the middle of the upper portion of the pressing plate 21, and the upper portion of the telescopic rod 22 is embedded in the driving assembly 3.
Furthermore, a plurality of tube slots are arranged on the PCR tube plate 4, the upper surface of the PCR tube plate 4 is adhered with a branch guide plate 41, the size and the position of the slot hole on the branch guide plate 41 correspond to the size and the position of the tube slot, and arc-shaped slots are arranged at the edges of the left side and the right side of the branch guide plate 41 on the PCR tube plate 4. The PCR tube plate 4 is made of metal materials and has high rigidity and supporting force. The PCR tube plate 4 is provided with a plurality of slots, in this embodiment, a single swab small PCR tube is distributed with 8-12 slots, and in other embodiments, other slot numbers can be distributed according to the size of the slots.
As a further improvement, the driving assembly 3 is a linear driver, and is composed of a motor push rod and a controller, the motor push rod is fixedly connected with the telescopic rod 22, and the controller is provided with an ECU and a hall sensor for monitoring the rotating speed of the motor at any time. When the Hall sensor monitors that the rotating speed of the motor is obviously changed, corresponding information is transmitted to the ECU, so that the ECU sends an instruction to a relay in the motor to enable the motor to rotate reversely, and the pressing plate 21 in a pressing state is lifted again; when the pressing plate 21 is pressed down by the ECU arranged on the motor, the pressing plate 21 is extruded when being contacted with the PCR tube sealing cover, resistance is generated, when the resistance is greater than the calibrated value of the ECU, the motor is enabled to generate reverse rotation, because the required resistance is in direct proportion according to the number of PCR tubes on the PCR tube plate 4, when the number of the PCR tubes is more, the required resistance is higher; the lower the resistance required when the number of PCR tubes is smaller.
As a further improvement, the rack 1 includes an upper top plate 11, a bottom plate 12 and a side plate 13, the side plate 13 is disposed on the same side of the upper top plate 11 and the bottom plate 12, the lower side of the upper top plate 11 is fixedly connected with the driving assembly 3, two guide bars 121 are disposed in parallel above the bottom plate 12, two PCR tube trays 4 are slidably disposed between the guide bars 121 above the bottom plate 12, the surface of the region between the two guide bars 121 on which the bottom plate 12 is disposed is smooth, the limiting sleeve 24 is composed of two hollow cylinders, and the diameter of the lower cylinder is greater than that of the upper cylinder. Wherein, the frame 1 outside is wrapping up casing 5, casing 5 side is equipped with the rectangle notch, the rectangle notch position corresponds with PCR tube dish 4 placement position.
The utility model discloses a concrete use:
in the using process, the PCR tube loaded with the reaction system and the amplification template is placed on the PCR tube disk 4 after being covered or filmed, and the PCR tube disk 4 is inserted into the bottom plate 12 between the two conducting bars 121, so that the PCR tube disk 4 can easily and stably slide into the bottom plate 12 through the smoother surface of the middle part of the bottom plate 12;
when the PCR tube disk is arranged under the pressing plate 21, the driving assembly controls the telescopic rod 22 to push the pressing plate 21 to vertically press down, and the pressing plate 21 can stably press the sealing covers of a plurality of PCR tubes carrying nucleic acid swabs into the tubes;
when the PCR pipe accomplishes the closing cap, clamp plate 21 reaches the predetermined farthest area of pushing down, at this moment, hall sensor in the motor monitors that motor speed appears obvious change, can be to the corresponding information of ECU transmission in the motor, thereby make ECU to the relay in the motor give-out order, make the motor reversal, and then shift up through telescopic link 22 control clamp plate 21, exert pressure through the region nature, the not totally closed state of objective application of force that appears when can effectively avoid manual pipe pressing, improve the security of swab storage in-process.
The basic principles and the main features of the invention and the advantages of the invention have been shown and described above, it will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, but that the invention may be embodied in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.