CN216449271U

CN216449271U - Sample adding device

Info

Publication number: CN216449271U
Application number: CN202122367746.4U
Authority: CN
Inventors: 罗丹; 吕杨; 陈昭明; 陈天垠; 孙爱宝; 徐春丽; 尤伟杰; 纪新凤; 王丽华
Original assignee: Hainan Kingmed Diagnostics Center Co ltd
Current assignee: Hainan Kingmed Diagnostics Center Co ltd
Priority date: 2021-09-28
Filing date: 2021-09-28
Publication date: 2022-05-06
Anticipated expiration: 2031-09-28

Abstract

The utility model discloses a sample adding device, which comprises a sample adding tube, a rotating tube, a power assembly and a switch, wherein the sample adding tube is arranged on the rotating tube; the sample adding pipe is provided with a sample inlet end and a sample outlet end; the first end of the rotating tube is movably connected with the sample outlet end of the sample adding tube, the second end of the rotating tube extends out of the sample adding tube, and the sample adding tube is communicated with the rotating tube; the power assembly drives the rotating pipe to rotate by taking the central shaft of the power assembly as the center through switch control. This application of sample device is through adding appearance pipe and rotatory pipe cooperation, can make reagent like hypotonic liquid add the sample to the reagent container behind adding appearance pipe, the rotatory pipe in proper order, and make this application of sample device be applied to the problem that chromosome karyotype result and quality all are unstable or even the experiment fails that can solve because of the operation gimmick of difference causes in the hypotonic liquid application of sample in-process through rotatory pipe and adding appearance pipe clearance fit to can reduce mixing operating time. Therefore, the sample adding device can perform sample adding operation and stirring operation, can improve the overall experimental efficiency, and reduces the volume of equipment.

Description

Sample adding device

Technical Field

The utility model belongs to the technical field of biological equipment, and particularly relates to a sample adding device.

Background

Chromosomes are structures that appear during mitosis of a cell visible under a microscope. Therefore, it is necessary to obtain a chromosome specimen for examination and analysis, and karyotype analysis is generally performed using peripheral blood lymphocytes. Normally, human peripheral blood lymphocytes no longer divide, but Phytohemagglutinin (PHA) stimulates the conversion of blood lymphocytes into lymphoblasts, restoring their proliferative capacity. Therefore, a small amount of peripheral venous blood can be adopted for short-term culture, the culture is carried out for 72 hours, the cells enter a proliferation vigorous stage, colchicine is added at the moment to inhibit cell division, the cell division is stopped at a metaphase so as to obtain a sufficient amount of cells in a division stage, and karyotype analysis is carried out by hypotonic, fixation, sheet preparation and observation under a dyeing mirror. Hypotonic effects are one of the most critical steps in the chromosome preparation process, and mainly cause cells to absorb water and fully swell. The principle is that the cell membrane of living cell is a semipermeable membrane, and the intracellular solute is higher than Kcl of low-osmotic-liquid concentration 0.075M, so that the extracellular water enters the cell from the outside of the cell by utilizing solute difference, the cell absorbs water and swells, and the foundation is laid for the final cell rupture and the full chromosome dispersion.

Currently, hypotonic treatment in laboratories is usually performed by blowing hypotonic solution for many times by using a rubber head dropper so that cells are separated and fully mixed with the hypotonic solution. However, during the operation, the following problems are liable to occur: the blowing process using a rubber head dropper needs extremely strong skill, and the blowing process needs to be utilized to generate large bubbles in the cell suspension, so that the generated shock wave can fully disperse the cells and reduce the possibility of breaking and dying the living cells as much as possible. In actual operation, the ideal operation level can be achieved by repeated practice, and most of the operations can cause cell rupture and death due to small and dense foam generated by insufficient experience, thereby affecting the preparation of chromosomes. Moreover, the blowing operation with insufficient proficiency usually suggests that the blowing time is properly prolonged, and the operation sometimes affects the whole experiment progress and is tedious to operate.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a sample adding device, which is used for solving the problems that the operation requirement of blowing cell suspension by adopting a rubber head dropper in the prior art is high, small and thick foams are easily generated in the blowing process to cause cell rupture and death, and the operation is complicated.

The technical scheme is as follows:

a sample adding device comprises a sample adding tube, a rotating tube, a power assembly and a switch;

the sample adding pipe is provided with a sample inlet end and a sample outlet end;

the first end of the rotating tube is movably connected with the sample outlet end of the sample adding tube, the second end of the rotating tube extends out of the sample adding tube, and the sample adding tube is communicated with the rotating tube;

the power assembly drives the rotating pipe to rotate by taking the central shaft of the rotating pipe as the center through the control of the switch.

In one embodiment, the sample adding device further comprises a holding part connected with the sample feeding end of the sample adding pipe, and the holding part is provided with an accommodating cavity;

the power assembly comprises a rotating motor and a rotating shaft, the rotating motor is arranged in the accommodating cavity of the holding part, the rotating shaft is arranged in the sample adding pipe, a first end of the rotating shaft is connected with the rotating motor, and a second end of the rotating shaft is connected with a first end of the rotating pipe.

In one embodiment, the sample adding pipe and the rotating pipe are coaxially arranged, and the rotating pipe is rotatably inserted into the sample outlet end of the sample adding pipe.

In one embodiment, a connecting piece is arranged on the inner peripheral wall of the rotating pipe close to the first end of the rotating pipe, the second end of the rotating shaft is connected with the connecting piece, a hollow part is arranged on the connecting piece, and the rotating pipe is communicated with the sample adding pipe through the hollow part.

In one embodiment, the switch is disposed on the holding portion, and the switch is a push switch.

In one embodiment, a rotation speed regulator and a gear number conversion button connected with the rotation speed regulator are further arranged on the holding part, and the rotation speed regulator controls the rotation speed of the rotating motor through the gear number conversion button.

In one embodiment, the holding portion is further provided with a flow rate adjusting button, and the sample adding tube controls the size of the sample outlet end through the flow rate adjusting button.

In one embodiment, the sample application tube has a storage cavity between the sample application end and the sample output end, and the radial length of the storage cavity is greater than the radial lengths of the sample application end and the sample output end.

In one embodiment, the sampling tube is provided with a graduation line arranged along the depth direction of the sampling tube between the storage cavity and the sampling end.

In one embodiment, the sample adding tube gradually decreases along the radial length from the storage cavity, the sample outlet end to the second end of the rotating tube in sequence.

The technical scheme provided by the utility model has the following advantages and effects:

this application of sample device is through adding appearance pipe and rotating tube cooperation, can make reagent if hypotonic liquid in proper order through adding the appearance pipe, application of sample to the reagent container behind the rotating tube, and through rotating tube and the cooperation of adding appearance pipe activity, can use rotating tube self center pin to rotate as the center through power component drive rotating tube after the application of sample is accomplished, stir with abundant mixing to the solution in the reagent container, make this application of sample device be applied to the hypotonic liquid application of sample in-process and can solve the chromosome karyotype result that causes because of the operation gimmick of difference and the unstable problem of experiment failure even of quality, and can reduce mixing operating time. Therefore, this application of sample device can carry out the application of sample operation, and can stir the operation, and easy operation is convenient, can promote whole experimental efficiency, reduces the equipment volume.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments of the utility model and, together with the description, serve to explain the principles and effects of the utility model.

Unless otherwise specified or defined, the same reference numerals in different figures refer to the same or similar features, and different reference numerals may be used for the same or similar features.

FIG. 1 is a schematic perspective view of a sample adding device according to an embodiment of the present invention;

FIG. 2 is a schematic diagram of a longitudinal cross-sectional structure of the sample adding device in FIG. 1;

FIG. 3 is a schematic top view of a connector of the sample addition device of FIG. 2.

Description of reference numerals:

100. a sample adding device;

1. a sample adding pipe; 11. a sample introduction end; 12. a storage cavity; 13. a sample outlet end; 14. scale lines; 2. rotating the tube; 21. a connecting member; 211. a hollow-out section; 212. an annular body; 213. a support member; 3. a power assembly; 31. a rotating electric machine; 32. a rotating shaft; 4. a switch; 5. a grip portion; 6. a gear number conversion button; 7. a flow rate adjustment button.

Detailed Description

In order to facilitate an understanding of the utility model, specific embodiments thereof will be described in more detail below with reference to the accompanying drawings.

Unless specifically stated or otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In the case of combining the technical solutions of the present invention in a realistic scenario, all technical and scientific terms used herein may also have meanings corresponding to the purpose of achieving the technical solutions of the present invention.

As used herein, unless otherwise specified or defined, "first" and "second" … are used merely for name differentiation and do not denote any particular quantity or order.

As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items, unless specified or otherwise defined.

It will be understood that when an element is referred to as being "secured to" another element, it can be directly secured to the other element or intervening elements may also be present; when an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present; when an element is referred to as being "mounted on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "on" another element, it can be directly on the other element or intervening elements may also be present.

The utility model provides a sample adding device 100, as shown in fig. 1 to 3, the sample adding device 100 comprises a sample adding tube 1, a rotating tube 2, a power assembly 3 and a switch 4.

The sample adding pipe 1 is provided with a sample inlet end 11 and a sample outlet end 13; the first end of the rotating pipe 2 is movably connected with the sample outlet end 13 of the sample adding pipe 1, the second end of the rotating pipe 2 extends out of the sample adding pipe 1, and the sample adding pipe 1 is communicated with the rotating pipe 2; in this embodiment, it can be understood that the sample adding device 100 flows the hypotonic solution through the sample inlet end 11 of the sample adding tube 1, and controls the sample adding tube 1 to make the sample outlet end 13 of the sample adding tube 1 flow out the solution, and the solution flows through the rotating tube 2 and then flows out from the second end of the rotating tube 2 to add the sample to the external device such as the reagent container.

The power assembly 3 controls and drives the rotating pipe 2 to rotate around the central shaft of the power assembly through the switch 4. It can be understood that, in this embodiment, after the sample loading is completed, the switch 4 is turned on, and the power assembly 3 drives the rotating tube 2 to rotate around the central axis of the rotating tube 2 itself, so as to stir the solution in the reagent container, such as the cell and the hypotonic solution, to mix them well.

It can be understood that, in the present embodiment, the usage principle of the sample adding device 100 is roughly as follows: with hypotonic liquid through the appearance end 11 entering of appearance pipe 1 with the appearance pipe 1 in, make the hypotonic liquid flow to rotatory pipe 2 through the appearance end 13 of appearance pipe 1 of appearance, and flow to the reagent container via rotatory pipe 2, after the application of sample is accomplished, open switch 4, power component 3 drive rotatory pipe 2 uses 2 self center pins of rotatory pipe to rotate as the center, stir cell and hypotonic liquid in the reagent container with abundant mixing.

In conclusion, this application of sample device 100 is through application of sample pipe 1 and the cooperation of rotating tube 2, can make reagent like hypotonic liquid in proper order through application of sample pipe 1, in the application of sample to the reagent container behind the rotating tube 2, and through rotating tube 2 and application of sample pipe 1 clearance fit, can drive rotating tube 2 through power component 3 after the application of sample is accomplished and use rotating tube 2 self center pin to rotate as the center, stir with abundant mixing to the solution in the reagent container, make this application of sample device 100 be applied to the problem that the chromosome karyotype result that causes because of different manipulation and quality are all unstable or even the experiment fails to can be solved to can reduce mixing operation time in the hypotonic liquid application of sample in-process. Therefore, this application of sample device 100 can carry out the application of sample operation, and easy operation is convenient, and can stir the operation, can promote whole experimental efficiency, reduces equipment volume.

In some embodiments, as shown in fig. 1 and fig. 2, the sample adding device 100 further includes a holding portion 5 connected to the sample injecting end 11 of the sample adding tube 1, and the holding portion 5 has a receiving cavity. The holding portion 5 is convenient for a user to grasp, so that the sample adding device 100 can be used conveniently. The power assembly 3 includes a rotating electrical machine 31 and a rotating shaft 32, the rotating electrical machine 31 is disposed in the accommodating cavity of the holding portion 5, the rotating shaft 32 is disposed in the sample adding tube 1, a first end of the rotating shaft 32 is connected to the rotating electrical machine 31, and a second end of the rotating shaft 32 is connected to the first end of the rotating tube 2. Understandably, through set up the portion of gripping 5 at the appearance end 11 of application of sample pipe 1, convenient to use person's gripping on the one hand, on the other hand is through accomodating rotating electrical machines 31 in the holding chamber in the portion of gripping 5, can improve overall structure's compactedness, avoid rotating electrical machines 31 to increase overall structure's volume, and avoid rotating electrical machines 31 to cause the interference to the rotation of rotating pipe 2, connect rotating electrical machines 31 and rotating pipe 2 respectively through rotation axis 32, can make the rotating electrical machines 31 that are located different positions drive the rotating pipe 2 rotatory.

In some embodiments, as shown in fig. 1, the holding portion 5 is plate-shaped, specifically, in this embodiment, the holding portion 5 is a rectangular plate, the edge of the holding portion 5 is rounded, and the holding portion 5 is perpendicular to the sample adding tube 1, so that the user can lift the sample adding tube quickly, and the gripping experience is improved.

In some embodiments, as shown in fig. 1, the sample application tube 1 and the rotary tube 2 are coaxially disposed, and the rotary tube 2 is rotatably inserted into the sample application end 13 of the sample application tube 1. Understandably, the sample adding pipe 1 and the rotating pipe 2 are coaxially arranged, so that the installation between the sample adding pipe 1 and the rotating pipe 2 is convenient, the arrangement of the rotating shaft 32 is convenient, and the solution such as the hypotonic solution can rapidly flow from the sample adding pipe 1 to the rotating pipe 2, so that the structure compactness is further improved. Of course, in this embodiment, the position where the rotary tube 2 and the sample application tube 1 relatively rotate and contact forms a sealing surface structure, so as to prevent the solution from flowing out from the position between the sample application tube 1 and the rotary tube 2. In addition, the outer wall of the rotating pipe 2 may be provided with an annular protrusion, and the inner wall of the sample adding pipe 1 may be provided with an annular groove, so that the rotating pipe 2 slides relative to the sample adding pipe 1 by the relative sliding of the annular protrusion in the annular groove. Of course, in other embodiments, the rotating tube 2 and the sample adding tube 1 may be rotatably inserted into the sample adding tube 1 through other structures, such as a bearing, so that the rotating tube 2 is inserted into the sample adding tube 1 and rotates relative to the sample adding tube 1, and is not limited herein.

In some embodiments, as shown in fig. 2 and 3, the inner peripheral wall of the rotary tube 2 near the first end of the rotary tube is provided with a connecting member 21, the second end of the rotary shaft 32 is connected to the connecting member 21, the connecting member 21 has a hollow portion 211, and the rotary tube 2 is communicated with the sample adding tube 1 through the hollow portion 211. It can be understood that the rotating tube 2 is connected to the rotating shaft 32 through the connecting member 21, so that the rotating shaft 32 drives the rotating tube 2 to rotate, and the hollow portion 211 on the connecting member 21 is used for circulating the solution, such as the hypotonic solution, so that the solution flows from the sample adding tube 1 to the rotating tube 2 through the hollow portion 211 and flows out, thereby avoiding blocking the solution circulation. Specifically, in this embodiment, the connecting member 21 includes an annular body 212 and a plurality of supporting members 213 extending along a radial direction of the annular body 212 and disposed at intervals, a distal end of each supporting member 213 extends to a center of the annular body 212 to overlap and form a joint, the second end of the rotating shaft 32 is connected to the joint, and a gap between adjacent supporting members 213 forms a hollow portion 211.

In some embodiments, as shown in fig. 1, the switch 4 is disposed on the grip portion 5, and the switch 4 is a push switch. Can understand ground, through pressing the press switch, can make power component 3 be in operating condition and drive the rotation of swivelling joint pipe 2 and stir in order to the solution in the reagent container, press once more after the stirring is accomplished and can stall, convenient to use person controls easy operation convenience gripping the in-process of portion 5 fast.

In some embodiments, as shown in fig. 1, a rotation speed regulator and a gear shift conversion button 6 connected to the rotation speed regulator are further disposed on the grip portion 5, and the rotation speed regulator controls the rotation speed of the rotating electrical machine 31 through the gear shift conversion button 6. It can be understood that the rotating speed of the rotating motor 31 for driving the rotating pipe 2 is adjusted by the rotating speed regulator, so that different stirring requirements can be met by adjusting to different rotating speeds, and the stirring quality and the stirring efficiency can be guaranteed.

In some embodiments, as shown in fig. 1, a flow rate adjusting button 7 is further disposed on the holding portion 5, and the sample adding tube 1 controls the size of the sample outlet end 13 through the flow rate adjusting button 7. It will be appreciated that the size of the outlet of the solution from the outlet end 13 of the sample application tube 1 can be adjusted by the flow rate adjustment button 7 to control the amount of the solution to be applied. Specifically, an electric valve can be arranged at the sample outlet of the sample adding pipe 1, and the electric valve is controlled by the flow rate adjusting button 7 to control the sample adding amount of the solution, so that the operation is simple and convenient. Of course, in other embodiments, other devices capable of adjusting the amount of the solution to be added may be applicable, and are not particularly limited herein.

In some embodiments, as shown in fig. 1, the sample adding tube 1 has a storage cavity 12 located between the sample inlet 11 and the sample outlet 13, and the radial length of the storage cavity 12 is greater than the radial lengths of the sample inlet 11 and the sample outlet 13, so that the sample adding tube 1 is located between the sample inlet 11 and the sample outlet 13 to expand to form the storage cavity 12, and the storage cavity 12 can store a certain volume of solution required by an experiment, thereby facilitating continuous sample adding by the sample adding device 100 and improving the sample adding effect. Specifically, in this embodiment, the storage cavity 12 is spherical, and the storage cavity 12 with smooth shape can avoid the solution residue and is convenient for flowing, thereby improving the aesthetic property.

In some embodiments, as shown in fig. 1, a graduation line 14 arranged along the depth direction of the sample application tube 1 is disposed between the storage cavity 12 and the sample output end 13 of the sample application tube 1, so that the remaining amount of the solution can be known in real time through the graduation line 14, thereby facilitating the operation of sample application and the like by a user according to the experiment requirement.

In some embodiments, as shown in fig. 1, the length of the sample adding tube 1 gradually decreases along the radial direction from the storage cavity 12 to the sample outlet end 13 to the second end of the rotating tube 2, the storage cavity 12, the sample outlet end 13 and the rotating tube 2 form a sample flowing channel, and the solution can accurately and densely flow out to an external reagent container through the gradually decreasing sample flowing channel, so as to avoid the solution from dispersing to form reagent waste.

In some embodiments, the sample inlet 11 or the storage cavity 12 further has a sample inlet communicating with the sample adding tube 1, the sample inlet is movably covered with a cover plate, the cover plate can be moved to cover the sample inlet to form a sealed state, the cover plate can be moved to open relative to the sample inlet to add or supplement the solution in the sample adding tube 1, and after the supplement is completed, the cover plate is moved to cover the sample inlet. It should be noted that, when the sample addition port is disposed at the sample introduction end 11, the sample addition port is disposed obliquely toward the sample introduction end 13, so as to facilitate sample introduction operation through the sample addition port, and of course, in other embodiments, the shape of the sample addition port is not limited herein.

When the drawing description is quoted, the new characteristics are explained; in order to avoid that repeated reference to the drawings results in an insufficiently concise description, the drawings are not referred to one by one in the case of clear description of the already described features.

The above embodiments are provided to illustrate, reproduce and deduce the technical solutions of the present invention, and to fully describe the technical solutions, the objects and the effects of the present invention, so as to make the public more thoroughly and comprehensively understand the disclosure of the present invention, and not to limit the protection scope of the present invention.

The above examples are not intended to be exhaustive of the utility model and there may be many other embodiments not listed. Any alterations and modifications without departing from the spirit of the utility model are within the scope of the utility model.

Claims

1. The sample adding device is characterized by comprising a sample adding tube, a rotating tube, a power assembly and a switch;

2. The sample adding device of claim 1, further comprising a holding portion connected to the sample inlet end of the sample adding tube, the holding portion having a receiving cavity;

3. The sample adding device according to claim 1, wherein the sample adding tube and the rotating tube are coaxially arranged, and the rotating tube is rotatably inserted into the sample outlet end of the sample adding tube.

4. The sample adding device according to claim 2, wherein the inner peripheral wall of the rotating tube near the first end of the rotating tube is provided with a connecting piece, the second end of the rotating tube is connected with the connecting piece, the connecting piece is provided with a hollow-out part, and the rotating tube is communicated with the sample adding tube through the hollow-out part.

5. The sample adding device according to claim 2, wherein the switch is disposed on the holding portion, and the switch is a push switch.

6. The sample adding device according to claim 2, wherein a rotation speed regulator and a step number conversion button connected with the rotation speed regulator are further arranged on the holding part, and the rotation speed regulator controls the rotation speed of the rotating motor through the step number conversion button.

7. The sample adding device according to claim 2, wherein a flow rate adjusting button is further provided on the holding portion, and the sample adding tube controls the size of the sample outlet end through the flow rate adjusting button.

8. The sample addition device of claim 1, wherein the sample addition tube has a storage cavity between the sample introduction end and the sample discharge end, and the radial length of the storage cavity is greater than the radial lengths of the sample introduction end and the sample discharge end.

9. The sample adding device according to claim 8, wherein the sample adding tube is provided with graduation lines arranged along the depth direction of the sample adding tube between the storage cavity and the sample outlet end.

10. The sample application device of claim 8, wherein the sample application tube gradually decreases in length along the radial direction from the storage cavity, the sample outlet end to the second end of the rotation tube.