CN207742002U - A kind of device with electric field acceleration tissue staining - Google Patents
A kind of device with electric field acceleration tissue staining Download PDFInfo
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- CN207742002U CN207742002U CN201721918223.1U CN201721918223U CN207742002U CN 207742002 U CN207742002 U CN 207742002U CN 201721918223 U CN201721918223 U CN 201721918223U CN 207742002 U CN207742002 U CN 207742002U
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- water pump
- staining
- staining trough
- dye liquor
- lid
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Abstract
The utility model discloses a kind of devices with electric field acceleration tissue staining, including：Pedestal, staining trough, staining trough lid, stepper motor, adapter, sample cage, sample cage lid, first electrode plate, second electrode plate, hose, the first water pump, the second water pump, waste liquid cylinder, constant temperature water tank and triple valve；Staining trough is fixed on pedestal；Staining trough lid is placed on staining trough；Sample cage is located in staining trough；Sample cage lid is placed on sample cage；Stepper motor is installed on staining trough lid, and is connect with sample cage by the adapter across staining trough lid；First electrode plate and second electrode plate are parallelly mounted in staining trough；First water pump is connected to constant temperature water tank and dye liquor entrance by hose；Triple valve for be connected to dye liquor outlet with the second water pump or be connected to dye liquor export and waste liquid cylinder.The utility model can effectively improve dyeing kinetics, and can realize the rapid dyeing to large volume biological tissue by applying DC voltage between two electrode plates.
Description
Technical field
The utility model belongs to bioengineering field, more particularly, to a kind of device with electric field acceleration tissue staining.
Background technology
Overflow dyeing machine is common a kind of equipment in medicine.But it is continuously increased with the workload of hospital, existing dyeing
The staining efficiency of machine can not meet actual demand.The prior art mostly uses the method for standing or liquid flowing to accelerate to give birth to
The ability of the dyeing of object tissue, acceleration is very limited, and is suitable only for the dyeing of thin sectioned tissue, as large volume tissue is micro-
The development of imaging tool has emerged some more outstanding imaging tools, such as sequence two-photon, supermicroscope
(mating plate), selection flat illumination microscope (mating plate), microoptic slice fault imaging, microoptic slice tomography fluorescence at
The appearance of picture, array fault imaging, these tools makes substantially the quick of product sample be prepared into for an active demand, large volume
The preparation of sample relates generally to transparency of organization or tissue staining, and biological tissue's especially brain tissue, very fine and close, dyestuff point
Son or marker are difficult Quick uniform dispersion in biological tissues, and it is very long therefore big to carry out the sample preparation period with the prior art
The rapid dyeing of volume biological tissue just seems extremely important, and at present at home, the Technical comparing of biological tissue's rapid dyeing
It is deficient, it is therefore desirable to a kind of method that biological tissue contaminates quickly to be filled up into this technological gap.
Utility model content
In view of the drawbacks of the prior art and Improvement requirement, the utility model provides a kind of with electric field acceleration tissue staining
Device its object is to effectively improve dyeing kinetics of the dyestuff to biological tissue, and can be fast implemented to large volume
The dyeing of biological tissue.
The utility model provides a kind of device with electric field acceleration tissue staining, including：Pedestal, staining trough, staining trough
Lid, stepper motor, adapter, sample cage, sample cage lid, first electrode plate, second electrode plate, the first water pump, the second water
Pump, waste liquid cylinder, constant temperature water tank and triple valve；Staining trough is fixed on pedestal, for containing dye liquor；Staining trough lid is placed on dye
On color slot；Sample cage is located in staining trough, for fettering biological tissue；Sample cage lid is placed on sample cage；Adapter passes through
The power output axis connection of circular hole among staining trough lid, one end and stepper motor, the adapter other end and sample cage lid
It is connected；Stepper motor is mounted on staining trough lid, for driving sample cage to rotate, accelerates the dyeing to biological tissue；The
One electrode plate and second electrode plate are parallelly mounted in staining trough, and one is used as cathode, another is as anode, for providing
Even electric field so that displacement occurs for electrically charged dye molecule, accelerates dyeing course；Constant temperature water tank is used to maintain stable
Dye liquor temperature；First water pump and the second water pump are for driving dye liquor to circulate；The input port of first water pump is connected to constant temperature
The output port of water tank, the first water pump is connected to the dye liquor entrance of staining trough side；The input port of second water pump is connected to three
The a port of port valve, the output port of the second water pump, which leads to, is connected to constant temperature water tank；Three ports of triple valve are respectively connected to
The input port of second water pump, the dye liquor outlet of staining trough side and waste liquid cylinder, triple valve have there are two gear, one of them
Gear makes dye liquor outlet be connected to the second water pump, another gear makes dye liquor outlet be connected to waste liquid cylinder.
Further, first electrode plate and the material of second electrode plate are Titanium so that first electrode plate and second
Electrode plate has stronger oxidation resistance, can carry out electrophoresis dying for a long time, and of low cost.
Further, adapter and sample cage lid respectively install in connecting portion there are one magnet so that adapter and sample
Product cage lid is reversibly linked together by magnetic force；Preferably, ferromagnetic material is Ru-Fe-Mn, magnet shape be cylinder or
Cuboid.
Further, there are one counterbores in one end that the power output shaft with stepper motor is connected setting for adapter, move
Power output shaft is inserted into counterbore so that adapter is linked together with power output shaft by interference fit.
Preferably, sample cage and sample cage lid establish matching relationship by internal and external threads.
Further, the input port of the first water pump is connected to constant temperature water tank, the output port of the first water pump by hose
The dye liquor entrance of staining trough side is connected to by hose；The output port of second water pump is connected to constant temperature water tank by hose；
Three ports of triple valve respectively by hose be connected to the input port of the second water pump, staining trough side dye liquor outlet and
Waste liquid cylinder；Preferably, hose is silicone tube or rubber tube.
Further, staining trough there are one or multiple dye liquor entrances and the outlet of one or more dye liquor；Dye liquor entrance and
Dye liquor outlet is respectively arranged at two opposite sides of staining trough, and the hole position height of both sides is different.
Further, sample cage side is provided with multiple windows, and dye liquor can enter sample cage from the window of sample cage side,
To which biological tissue be dyed.
Preferably, the first water pump is peristaltic pump, diaphragm pump or gear pump；Second water pump be peristaltic pump, diaphragm pump or
Gear pump.
In general, by the contemplated above technical scheme of the utility model, following advantageous effect can be obtained：
(1) electrically charged under electric field action for conductive dye liquor itself by applying voltage to electrode plate
Dye molecule occur directional migration, pass through biological tissue speed accelerate, to accelerate dyeing course；
(2) dyeing kinetics are fast, and the dyeing to large volume biological tissue can be realized within the acceptable time.
(3) it uses Ti electrode plate as electrode, is on the one hand capable of providing uniform electric field, another aspect titanium material against oxidative
Property is strong, of low cost.
Description of the drawings
Fig. 1 is the schematic device with electric field acceleration tissue staining that the utility model embodiment example provides；
Fig. 2 is the schematic diagram for the adapter that the utility model embodiment provides；
Fig. 3 is the staining trough internal structure schematic diagram that the utility model embodiment provides；
Fig. 4 is the sample cage schematic diagram that the utility model embodiment provides.
In all the appended drawings, identical reference numeral is used for indicating identical element or structure, wherein：
1 is pedestal, and 2 be staining trough, and 3 be staining trough lid, and 4 be stepper motor, and 5 be adapter, and 6 be the first water pump, 7
It is the second water pump, 8 be triple valve, and 9 be waste liquid cylinder, and 10 be hose, and 11 be constant temperature water tank, and 12 be first electrode plate, and 13 be second
Electrode plate, 14 be dye liquor entrance, and 15 be dye liquor outlet, and 16 be sample cage, and 17 be sample cage lid.
Specific implementation mode
In order to make the purpose of the utility model, technical solutions and advantages more clearly understood, below in conjunction with attached drawing and implementation
Example, the present invention will be further described in detail.It should be appreciated that specific embodiment described herein is only used to explain
The utility model is not used to limit the utility model.In addition, institute in the various embodiments of the present invention described below
The technical characteristic being related to can be combined with each other as long as they do not conflict with each other.
The device with electric field acceleration tissue staining that the utility model provides, as shown in Figure 1, including：Pedestal 1, dyeing
Slot 2, staining trough lid 3, stepper motor 4, adapter 5, sample cage 16, sample cage lid 17, first electrode plate 12, second electrode
Plate 13, the first water pump 6, the second water pump 7, waste liquid cylinder 9, constant temperature water tank 11 and triple valve 8；Staining trough 2 is fixed on pedestal 1,
For containing dye liquor；Staining trough lid 3 is placed on staining trough 2；Sample cage 16 is located in staining trough 2, for fettering biological tissue；
Sample cage lid 17 is placed on sample cage 16；Adapter 5 passes through the circular hole among staining trough lid 3, one end and stepper motor 4
Power output axis connection so that stepper motor is mounted on staining trough lid 3, and the other end is connected with sample cage lid 17；Step
Stepper motor accelerates the dyeing to biological tissue for driving sample cage 16 to rotate；First electrode plate 12 and second electrode plate 13 are flat
Row is installed in staining trough 2, and one is used as cathode, another is as anode, for providing uniform electric field so that electrically charged
Displacement occurs for dye molecule, accelerates dyeing course；Constant temperature water tank 11 is for maintaining stable dye liquor temperature；First water pump 6
With the second water pump 7 for driving circulating for dye liquor；The input port of first water pump 6 is connected to constant temperature water tank 11, the first water
The output port of pump 6 is connected to the dye liquor entrance 14 of 2 side of staining trough；The input port of second water pump 7 is connected to triple valve 8
The output port of a port, the second water pump 7 is connected to constant temperature water tank 11；Three ports of triple valve 8 are respectively connected to second
The input port of water pump 7, the dye liquor outlet 15 of 2 side of staining trough and waste liquid cylinder 9, there are two gears for the tool of triple valve 8, wherein
One gear makes dye liquor outlet 15 be connected to the second water pump 7, another gear makes dye liquor outlet 15 connect with waste liquid cylinder 9
It is logical.
In the present embodiment, the input port of the first water pump 6 is connected to constant temperature water tank 11, the first water pump 6 by hose 10
Output port the dye liquor entrance of 2 side of staining trough is connected to by hose 10；The output port of second water pump 7 passes through hose 10
It is connected to constant temperature water tank 11；Three ports of triple valve 8 are connected to input port, the dye of the second water pump 7 by hose 10 respectively
The dye liquor of 2 side of color slot exports and waste liquid cylinder 9；Preferably, hose 10 is silicone tube or rubber tube.
As shown in Fig. 2, one end of adapter 5, there are one counterbore, the power output shaft of stepper motor 4 is inserted into the counterbore, and
It is linked together with adapter 5 by being interference fitted；There are one magnet for the other end bonding of adapter 5；As shown in figure 4, sample
Cage lid 17 is placed on by way of screw-thread fit on sample cage 16, and there are one magnet for the bonding of sample cage lid 17；Adapter 5 with
Sample cage lid 17 links together by the way that the magnetic force of magnet is reversible.
As shown in figure 3, first electrode plate 12 and second electrode plate 13 are parallelly mounted in staining trough 2, staining trough one
Side is provided with dye liquor entrance 14, and opposite side is provided with dye liquor outlet 15, the vacancy height of dye liquor entrance 14 and dye liquor outlet 15
It is different.
As shown in figure 4,16 side of sample cage is provided with 4 windows, the central angle of each window is 40 °~80 °, biological tissue
It is bound in sample cage 16, dye liquor can enter sample cage 16 from side window, to dye biological tissue.
When dyeing, dye liquor is filled it up in staining trough 2 and constant temperature water tank 11, covers staining trough lid 3；Open stepper motor 4
Power switch, the swing circle of stepper motor 4 is 5~40min；The gear of triple valve 8 is set so that dye liquor outlet 14 and the
Two water pumps 7 are connected to, and open the power switch of the first water pump 6 and the second water pump 7, and driving dye liquor circulates；Until dye liquor flows
After stabilization, apply direct current between first electrode plate 12 and second electrode plate 13, voltage is 20~100V, is started to biology
Tissue staining；Dyeing finishes, and opens staining trough lid 3, takes out dyed biological tissue, and the gear of triple valve 8 is arranged so that
Dye liquor outlet 15 is connected to waste liquid cylinder, and dye liquor in pipeline is discharged into waste liquid cylinder 9.
Two similar Mice brain tissues are taken, one is dyed under conditions of standing, and one uses the utility model
What is provided is dyed with the device of electric field acceleration tissue staining, and two brain tissues use identical dye liquor, dyeing time and temperature
To spend identical, dyeing finishes, and cuts brain tissue along brain tissue maximum coronal-plane, and the brain tissue on the left side uses standing dyeing,
The brain tissue on the right uses electrophoresis dying, and darker regions are to be colored region.The right brain tissue peripheral color obviously compares the left side
Brain tissue is deep, illustrates that the right brain tissue coloring is more abundant, therefore, at same time, same temperature, the utility model embodiment
The miniature ultrasonic biological tissue dyeing apparatus of offer can accelerate dyeing course, have apparent advantageous effect.
As it will be easily appreciated by one skilled in the art that the above is only the preferred embodiment of the utility model only, not
To limit the utility model, any modification made within the spirit and principle of the present invention, equivalent replacement and change
Into etc., it should be included within the scope of protection of this utility model.
Claims (8)
1. a kind of device with electric field acceleration tissue staining, including：Pedestal, staining trough, staining trough lid, stepper motor, switching
Head, sample cage, sample cage lid, first electrode plate, second electrode plate, the first water pump, the second water pump, waste liquid cylinder, constant temperature water tank
And triple valve；The staining trough is fixed on the pedestal；The staining trough lid is placed on the staining trough；The sample
Cage is located in the staining trough；The sample cage lid is placed on the sample cage；The adapter passes through the dyeing capping
Sub intermediate circular hole, the power output axis connection of one end and the stepper motor, the other end are connected with the sample cage lid；
The stepper motor is mounted on the staining trough lid, for driving the sample cage to rotate, accelerates the dye to biological tissue
Color；The first electrode plate and the second electrode plate are parallelly mounted in the staining trough, and one is used as cathode, another work
For anode, for providing uniform electric field so that displacement occurs for electrically charged dye molecule, accelerates dyeing course；It is described
The input port of first water pump is connected to the constant temperature water tank, and the output port of first water pump is connected to the staining trough side
The dye liquor entrance in face；The input port of second water pump is connected to a port of the triple valve, second water pump
Output port is connected to the constant temperature water tank；Three ports of the triple valve are respectively connected to the input terminal of second water pump
Mouth, the dye liquor outlet of the staining trough side and the waste liquid cylinder；There are two gear, one of gears for the triple valve tool
So that the dye liquor outlet is connected to second water pump, another gear makes the dye liquor outlet connect with the waste liquid cylinder
It is logical.
2. using the device of electric field acceleration tissue staining as described in claim 1, which is characterized in that the first electrode plate and institute
The material for stating second electrode plate is Titanium.
3. using the device of electric field acceleration tissue staining as described in claim 1, which is characterized in that the adapter and the sample
Product cage lid is respectively installed in connecting portion there are one magnet so that the adapter and the sample cage lid are reversible by magnetic force
Ground links together.
4. as described in claim 1 use electric field acceleration tissue staining device, which is characterized in that the adapter with it is described
One end setting that the power output shaft of stepper motor is connected is there are one counterbore, and the power output shaft is inserted into counterbore so that institute
Adapter is stated to link together by interference fit with the power output shaft.
5. using the device of electric field acceleration tissue staining as described in claim 1, which is characterized in that the sample cage and the sample
Product cage lid establishes matching relationship by internal and external threads.
6. using the device of electric field acceleration tissue staining as described in claim 1, which is characterized in that the input of first water pump
Port is connected to the constant temperature water tank by hose, and the output port of first water pump is connected to the staining trough by hose
The dye liquor entrance of side；The output port of second water pump is connected to the constant temperature water tank by hose；The triple valve
Three ports respectively by hose be connected to the input port of second water pump, the staining trough side dye liquor outlet and
The waste liquid cylinder.
7. as described in claim 1 use electric field acceleration tissue staining device, which is characterized in that the staining trough there are one or
Multiple dye liquor entrances and one or more dye liquor outlets；The dye liquor entrance and dye liquor outlet are respectively arranged at the dye
Two opposite sides of color slot, and the hole position height of both sides is different.
8. using the device of electric field acceleration tissue staining as described in claim 1, which is characterized in that sample cage side is provided with
Multiple windows, dye liquor enter the sample cage from the window of sample cage side, to dye biological tissue.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721918223.1U CN207742002U (en) | 2017-12-29 | 2017-12-29 | A kind of device with electric field acceleration tissue staining |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721918223.1U CN207742002U (en) | 2017-12-29 | 2017-12-29 | A kind of device with electric field acceleration tissue staining |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207742002U true CN207742002U (en) | 2018-08-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201721918223.1U Active CN207742002U (en) | 2017-12-29 | 2017-12-29 | A kind of device with electric field acceleration tissue staining |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112557151A (en) * | 2020-12-21 | 2021-03-26 | 哈尔滨格林标本技术开发有限公司 | Biological tissue dyeing machine |
-
2017
- 2017-12-29 CN CN201721918223.1U patent/CN207742002U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112557151A (en) * | 2020-12-21 | 2021-03-26 | 哈尔滨格林标本技术开发有限公司 | Biological tissue dyeing machine |
| CN112557151B (en) * | 2020-12-21 | 2024-05-10 | 哈尔滨格林标本技术开发有限公司 | Biological tissue dyeing machine |
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