CN216445358U

CN216445358U - Peripheral blood lymphocyte chromosome aberration detection kit

Info

Publication number: CN216445358U
Application number: CN202123212130.6U
Authority: CN
Inventors: 刘冬明
Original assignee: Xinjiang Prosai New Medical Laboratory Co ltd
Current assignee: Xinjiang Prosai New Medical Laboratory Co ltd
Priority date: 2021-12-20
Filing date: 2021-12-20
Publication date: 2022-05-06
Anticipated expiration: 2031-12-20

Abstract

The utility model discloses a peripheral blood lymphocyte chromosome aberration detection kit, which is provided with a box body, wherein an annular groove is fixedly connected on the inner side wall of the box body, a bearing plate is rotatably connected on the annular groove, a plurality of clamping holes are annularly formed on the bearing plate, filling devices are clamped in the clamping holes, detection reagents required for detection are respectively filled in the plurality of filling devices, a screwing column is fixedly connected on the bearing plate and positioned at the center, a through hole is formed in the lower part of the box body, a culture dish is inserted into the box body through the through hole, a partition plate is arranged in the culture dish and divides the culture dish into a plurality of culture sub-grooves, the bearing plate is driven to rotate by rotating the screwing column, the plurality of filling devices rotate along with the filling devices, the corresponding filling devices are rotated to the upper sides of the corresponding culture sub-grooves according to requirements, the detection reagents are filled into the culture sub-grooves through the filling devices, and the kit can be convenient for a detection person to add a plurality of detection reagents into the culture dish, the operation process of detection is simplified, and the detection time is shortened.

Description

Peripheral blood lymphocyte chromosome aberration detection kit

Technical Field

The utility model relates to the technical field of kits, in particular to a peripheral blood lymphocyte chromosome aberration detection kit.

Background

When the cells are subjected to mutagens in G0 or G1, chromosomes are not replicated, single chromosomes are shot and broken, and after S-phase replication, 2 chromatids in the metaphase (M) show structural changes in the same position. According to the in vivo outcome of the aberration, the chromosome aberration can be divided into unstable chromosome aberration and stable chromosome aberration, wherein the unstable chromosome aberration mainly comprises double centromeres, centromeres rings, no centromeres fragments and micro-particles, and ar, f and min are collectively called as non-centromeres and the like; the latter mainly include reciprocal translocations, inversions and deletions, etc. The kit for detecting the chromosome aberration of the peripheral blood lymphocytes mainly detects people irradiated by electric radiation in a radiology work unit in the radiology work activity. In the process of detecting the chromosome aberration of the peripheral blood lymphocytes, various reagents need to be added into a culture medium, and the adding operation is complex, so that the detection time is prolonged, and the kit for detecting the chromosome aberration of the peripheral blood lymphocytes is provided.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a kit for detecting chromosome aberration of peripheral blood lymphocytes, which solves the problems in the background technology.

In order to achieve the purpose, the utility model provides the following technical scheme: the peripheral blood lymphocyte chromosome aberration detection kit comprises a box body, wherein an annular groove is fixedly connected on the inner side wall of the box body, a bearing plate is rotatably connected on the annular groove, the box body is a transparent box body, the bearing plate is a transparent plate body and is convenient for observing the adding condition of a detection reagent in the box body, the peripheral side of the bearing plate is a connecting groove matched with the annular groove, the two grooves are connected, so that the bearing plate can rotate on the annular groove under the action of external force, a plurality of clamping holes are formed in the bearing plate along the annular shape, filling devices are clamped in the clamping holes, the plurality of filling devices are respectively filled with the detection reagent required by detection, corresponding reagent marks are respectively arranged on the plurality of filling devices so as to be distinguished by detection personnel, a twisting column is fixedly connected on the bearing plate and positioned at the center, and a through hole is formed in the lower part of the box body, the culture dish is inserted into the box body through the through hole, a partition plate is arranged in the culture dish and divides the culture dish into a plurality of culture sub-grooves, the bearing plate is driven to rotate by rotating the screwing column, a plurality of filling devices rotate along with the bearing plate, the corresponding filling devices rotate to the upper sides of the corresponding culture sub-grooves according to needs, and detection reagents are filled into the culture sub-grooves through the filling devices.

Preferably, the filling device comprises a filling pipe, a limiting ring is fixedly connected to the outer side wall of the filling pipe, a piston is arranged in the filling pipe, a pressing rod is connected to the piston, a liquid outlet head is communicated with the lower end of the filling pipe, the filling pipe is clamped in the clamping hole, the limiting ring abuts against the bearing plate to prevent the filling pipe from moving downwards, a detection reagent in the filling pipe is pushed out by pressing the pressing rod, and scale marks are arranged on the filling pipe to facilitate quantitative addition.

Preferably, the upper end of the filling pipe is fixedly connected with an annular pull handle, and the annular pull handle is arranged to be convenient for the forefinger and the middle finger to abut against, so that the thumb can press the compression bar with force.

Preferably, the upper end opening part of box body threaded connection has the closing cap, the setting of closing cap makes the whole portable of box body.

Preferably, the division board comprises a plurality of plate bodys, and a plurality of plate bodys are in along annular evenly distributed in the culture dish, and the cultivation of multiunit culture medium can be carried out to a plurality of cultivation minute grooves to the cultivation that separates is fan-shaped, and the culture medium contrast observation in the testing process of being convenient for.

Preferably, the outer side wall of the culture dish is fixedly connected with a handle, and the handle is arranged to facilitate moving the culture dish.

Preferably, terminal surface fixedly connected with draw-in groove under the diapire of box body, the draw-in groove is used for the joint the culture dish, when the whole messenger that needs to carry of kit, can with the culture dish card is in the draw-in groove, whole portable.

Compared with the prior art, the utility model has the beneficial effects that: the culture dish box is provided with a box body, an annular groove is fixedly connected on the inner side wall of the box body, a bearing plate is rotatably connected on the annular groove, the box body is a transparent box body, the bearing plate is a transparent plate body and is convenient for observing the adding condition of a detection reagent in the box body, the peripheral side of the bearing plate is provided with a connecting groove matched with the annular groove, the two grooves are connected, so that the bearing plate can rotate on the annular groove under the action of external force, a plurality of clamping holes are formed in the bearing plate along the annular shape, filling devices are clamped in the clamping holes, the plurality of filling devices are respectively filled with the detection reagent required by detection, the plurality of filling devices are respectively provided with corresponding reagent marks so as to be distinguished by detection personnel, a screwing column is fixedly connected on the bearing plate and positioned at the center, a through hole is formed in the lower part of the box body, a culture dish is inserted into the box body through the through hole, a partition plate is arranged in the culture dish, the partition plate divides the culture dish into a plurality of culture sub-grooves, and drives the bearing plate to rotate by rotating the screwing column, the plurality of filling devices rotate along with the culture medium, the corresponding filling devices rotate to the upper sides of the corresponding culture sub-tanks according to needs, and the detection reagents are filled into the culture sub-tanks through the filling devices.

Drawings

FIG. 1 is a sectional view of the main structure of the present invention;

FIG. 2 is a schematic view of the main structure of the present invention;

FIG. 3 is a schematic view of a carrier plate according to the present invention;

FIG. 4 is a top view of a culture dish according to the utility model.

In the figure: 1-box body, 2-annular groove, 3-bearing plate, 4-clamping hole, 5-filler, 501-filler pipe, 502-limit ring, 503-piston, 504-compression bar, 505-liquid outlet head, 506-annular pull handle, 6-screwing column, 7-through hole, 8-culture dish, 9-separation plate, 10-sealing cover, 11-handle and 12-clamping groove.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-4, the present invention provides the following technical solutions: the peripheral blood lymphocyte chromosome aberration detection kit comprises a box body 1, wherein an annular groove 2 is fixedly connected on the inner side wall of the box body 1, a bearing plate 3 is rotatably connected on the annular groove 2, the box body 1 is a transparent box body, the bearing plate 3 is a transparent plate body and is convenient for observing the adding condition of a detection reagent in the box body 1, the peripheral side of the bearing plate 3 is a connecting groove matched with the annular groove 2, the two grooves are connected, so that the bearing plate 3 can rotate on the annular groove 2 under the action of external force, a plurality of clamping holes 4 are formed in the bearing plate 3 along the annular shape, a filling device 5 is clamped in the clamping holes 4, the filling devices 5 are respectively filled with the detection reagent required for detection, corresponding reagent marks are respectively arranged on the filling devices 5 so as to be distinguished by detection personnel, and a twisting column 6 is fixedly connected on the bearing plate 3 and positioned at the center, the kit is characterized in that a through hole 7 is formed in the lower portion of the box body 1, a culture dish 8 is inserted into the box body 1 through the through hole 7, a partition plate 9 is arranged in the culture dish 8, the partition plate 9 divides the culture dish into a plurality of culture sub-grooves, the screwing column 6 is rotated to drive the bearing plate 3 to rotate, a plurality of filling devices 5 rotate along with the partition plate, the corresponding filling devices 5 are rotated to the upper sides of the corresponding culture sub-grooves according to needs, and detection reagents are filled into the culture sub-grooves through the filling devices 5.

Specifically, the filling device 5 comprises a filling pipe 501, a limit ring 502 is fixedly connected to the outer side wall of the filling pipe 501, a piston 503 is arranged in the filling pipe 501, a pressing rod 504 is connected to the piston 503, a liquid outlet 505 is communicated with the lower end of the filling pipe 501, the filling pipe 501 is clamped in the clamping hole 4, the limit ring 502 abuts against the bearing plate 3 to prevent the filling pipe 501 from moving downwards, a detection reagent in the filling pipe 501 is pushed out by pressing the pressing rod 504, and scale marks are arranged on the filling pipe 501 to facilitate quantitative addition.

Specifically, an annular pull handle 506 is fixedly connected to the upper end of the fill pipe 501, and the annular pull handle 506 is arranged to be convenient for the forefinger and the middle finger to abut against, so that the thumb can press the pressure lever 504 with force.

Particularly, the upper end opening part threaded connection of box body 1 has closing cap 10, the setting of closing cap 10 makes the whole portable of box body 1.

Particularly, division board 9 comprises a plurality of plate bodys, and a plurality of plate bodys are in along annular evenly distributed in the culture dish 8, the cultivation of separating divides the groove to be fan-shaped, and a plurality of cultivation divides the groove to carry out the cultivation of multiunit culture medium, and the culture medium contrast observation in the testing process of being convenient for.

Specifically, fixedly connected with handle 11 on the lateral wall of culture dish 8, the setting of handle 11 is convenient for remove culture dish 8.

Particularly, terminal surface fixedly connected with draw-in groove 12 under the diapire of box body 1, draw-in groove 12 is used for the joint culture dish 8, when the whole messenger that needs to carry of kit, can with culture dish 8 card is in draw-in groove 12, whole portable.

The working principle is as follows: the detection kit is provided with a kit body 1, an annular groove 2 is fixedly connected on the inner side wall of the kit body 1, a bearing plate 3 is rotatably connected on the annular groove 2, the kit body 1 is a transparent kit body, the bearing plate 3 is a transparent plate body, the adding condition of a detection reagent in the kit body 1 can be observed conveniently, the circumferential side of the bearing plate 3 is a connecting groove matched with the annular groove 2, the two grooves are connected, so that the bearing plate 3 can rotate on the annular groove 2 under the action of external force, a plurality of clamping holes 4 are formed on the bearing plate 3 along the annular shape, filling devices 5 are clamped in the clamping holes 4, the detection reagents required for detection are respectively arranged in the filling devices 5, corresponding reagent marks are respectively arranged on the filling devices 5 for distinguishing by detection personnel, a screwing column 6 is fixedly connected on the bearing plate 3 and positioned at the center, a through hole 7 is formed in the lower part of the kit body 1, a culture dish 8 is inserted into the kit body 1 through the through hole 7, and a partition plate 9 is arranged in the culture dish 8, division board 9 separates a plurality of cultivation minute grooves with the culture dish, twists post 6 through rotating and drive loading board 3 and rotate, and a plurality of filler 5 rotate along with it, rotates corresponding filler 5 to corresponding cultivation minute groove upside as required, annotates detection reagent through filler 5 to cultivation minute inslot, and this kit can conveniently add multiple detection reagent in the testing personnel adds to the culture dish, makes the operation process of detection simplify, shortens check-out time.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims

1. Peripheral blood lymphocyte chromosome aberration detection kit, its characterized in that: the detection kit comprises a kit body (1), wherein an annular groove (2) is fixedly connected to the inner side wall of the kit body (1), a bearing plate (3) is rotatably connected to the annular groove (2), a plurality of clamping holes (4) are formed in the bearing plate (3) along the annular direction, a filling device (5) is clamped in the clamping holes (4), a plurality of detection reagents required by the filling device (5) are respectively arranged in the filling device (5), a screwing column (6) is fixedly connected to the bearing plate (3) and positioned at the center, a through hole (7) is formed in the lower portion of the kit body (1), a culture dish (8) is inserted into the kit body (1) through the through hole (7), a separation plate (9) is arranged in the culture dish (8), the culture dish is separated into a plurality of culture sub-grooves by the separation plate (9), the bearing plate (3) is driven to rotate by rotating the screwing column (6), and the plurality of filling devices (5) rotate along with the separation plate, and rotating the corresponding filling device (5) to the upper side of the corresponding culture sub-tank as required, and filling the detection reagent into the culture sub-tank through the filling device (5).

2. The kit for detecting chromosomal aberration in peripheral blood lymphocytes according to claim 1, wherein: the filling device (5) comprises a filling pipe (501), a limiting ring (502) is fixedly connected to the outer side wall of the filling pipe (501), a piston (503) is arranged in the filling pipe (501), a compression bar (504) is connected to the piston (503), and the lower end of the filling pipe (501) is communicated with a liquid outlet head (505).

3. The kit for detecting chromosomal aberration in peripheral blood lymphocytes according to claim 2, wherein: the upper end of the filling pipe (501) is fixedly connected with an annular pull handle (506).

4. The kit for detecting chromosomal aberration in peripheral blood lymphocytes according to any one of claims 1 to 3, wherein: the upper end opening of the box body (1) is in threaded connection with a sealing cover (10).

5. The kit for detecting chromosomal aberration in peripheral blood lymphocytes according to any one of claims 1 to 3, wherein: the partition plate (9) is composed of a plurality of plate bodies which are uniformly distributed in the culture dish (8) along the ring shape.

6. The kit for detecting chromosomal aberration in peripheral blood lymphocytes according to claim 5, wherein: the outer side wall of the culture dish (8) is fixedly connected with a handle (11).

7. The kit for detecting chromosomal aberration in peripheral blood lymphocytes according to claim 6, wherein: terminal surface fixedly connected with draw-in groove (12) under the diapire of box body (1), draw-in groove (12) are used for the joint culture dish (8).