CN216440665U - Multifunctional liquid reaction device - Google Patents
Multifunctional liquid reaction device Download PDFInfo
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- CN216440665U CN216440665U CN202123080673.7U CN202123080673U CN216440665U CN 216440665 U CN216440665 U CN 216440665U CN 202123080673 U CN202123080673 U CN 202123080673U CN 216440665 U CN216440665 U CN 216440665U
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Abstract
The utility model discloses a multifunctional liquid reaction device, which comprises a passage switching unit, an injection unit, a liquid storage unit, a reaction unit and a control unit, wherein the injection unit is used for injecting liquid; the injection unit is connected to the reservoir unit; the passage switching unit, the injection unit and the reaction unit are respectively in communication connection with the control unit. The application provides a multi-functional liquid reaction unit can provide accurate, automatic, efficient experimental apparatus for the chemical detection experiment. The functions of accurate liquid feeding, participation of various liquids in reaction, constant-temperature heating (time and temperature are controllable and can be set), reaction, automatic treatment of experimental results and the like can be realized. Can realize the accurate quantitative automatic injection and automatic cleaning of various reagents. The use of the in-situ constant temperature heating technology and the PID constant temperature heating technology ensures that the temperature control is more accurate, and the efficiency and the accuracy can be improved. Is worthy of large-area popularization and application.
Description
Technical Field
The utility model relates to the technical field of experimental instruments, in particular to a multifunctional liquid reaction device.
Background
In biochemical teaching or scientific research, an experimenter often needs to add 2 or more reagents to mix after carrying out certain biochemical experiment, or to stand or heat to a constant temperature, and keep the temperature for a certain time, and finally obtains an experimental result according to a certain phenomenon or concentration. In this process, the experimenter generally adds and mixes the liquids manually by using a test instrument such as a measuring cylinder, a measuring flask, and a dropper. The operation mode causes many artificial interference factors, and errors are generated randomly and uncontrollably along with the mood, the manipulation, the proficiency, the operation habit and the like of experimenters when reagents are added manually, so that the results are different under the same experiment condition, and even the errors are large.
Meanwhile, when a biochemical experiment is usually performed, a large number of repeated experiments are often performed on experiments with the same experimental conditions, and a large number of repeated works such as reagent addition, constant-temperature digestion, result judgment and the like also need to be manually participated, so that a large amount of workload is increased for experimenters.
In addition, in the biochemical experiment digestion process, a reagent is usually placed in a test tube and then is digested by a specific digestion instrument, the digestion temperature required by some experiments is higher, or some reagents have stronger corrosivity and toxicity, so that the test tube is dangerous in the moving process, and personnel are easily injured.
SUMMERY OF THE UTILITY MODEL
The utility model provides a multifunctional liquid reaction device. The device can realize the functions of accurate liquid feeding, participation of various liquids in reaction, constant-temperature heating (time and temperature are controllable and can be set), reaction automation, automatic treatment of experimental results and the like.
The utility model provides the following scheme:
a multi-functional liquid reaction device comprising:
the device comprises a passage switching unit, an injection unit, a liquid storage unit, a reaction unit and a control unit; the injection unit is connected to the reservoir unit; the passage switching unit, the injection unit and the reaction unit are respectively in communication connection with the control unit;
the passage switching unit comprises a common pipeline, a first branch pipeline and a plurality of second branch pipelines; the liquid storage unit is connected with the public pipeline, and the reaction unit is connected with the first branch pipeline; the second branch pipelines are respectively used for being connected with different solution containers in a one-to-one correspondence mode.
Preferably: the reaction unit comprises an inlet end and an outlet end, and the inlet end and the outlet end are respectively provided with a pressure relief valve; the pressure relief valve is in communication connection with the control unit; the control unit is used for sending the reaction strategy to the pressure release valve so that the pressure release valve can adjust the on-off state according to the reaction strategy.
Preferably: the reaction unit comprises a main body made of transparent materials, a photoelectric signal acquisition assembly is arranged on the main body and can be communicated with the control unit, and the control unit is used for judging the concentration of the reagent in the main body according to the condition that the reagent acquired by the photoelectric signal acquisition assembly absorbs light with a target wavelength.
Preferably: the photoelectric signal acquisition assembly comprises a light source and a photocell which are oppositely arranged on the main body, and the reaction unit further comprises a shading shell which is arranged on the outer side of the main body.
Preferably: the reaction unit comprises a temperature control component, the temperature control component is in communication connection with the control unit, and the temperature control component is used for adjusting the temperature in the reaction unit according to a reaction strategy so as to form a target reaction environment in the reaction unit.
Preferably: the channel switching unit comprises a multi-channel switching valve island containing a switching power assembly, and the multi-channel switching valve island comprises a sapphire valve core and a valve head with a polytrifluoroethylene corrosion-resistant layer.
Preferably: the valve hole positioning of the multi-channel switching valve island adopts a coded disc optocoupler mode for positioning, and the switching power assembly comprises a planetary reduction gearbox motor.
Preferably: the injection unit comprises a vertical injection pump; the vertical injection pump is respectively connected with the liquid storage unit and the pure water supply unit comprising a pure water valve; the liquid storage unit comprises a liquid storage ring.
Preferably: the control unit comprises an STM32F373CBT6 single chip microcomputer, and is further connected with an acousto-optic alarm assembly.
According to the specific embodiment provided by the utility model, the utility model discloses the following technical effects:
according to the utility model, a multifunctional liquid reaction device can be realized, and in an implementation mode, the device can comprise a passage switching unit, an injection unit, a liquid storage unit, a reaction unit and a control unit; the injection unit is connected to the reservoir unit; the passage switching unit, the injection unit and the reaction unit are respectively in communication connection with the control unit; the passage switching unit comprises a common pipeline, a first branch pipeline and a plurality of second branch pipelines; the liquid storage unit is connected with the public pipeline, and the reaction unit is connected with the first branch pipeline; the second branch pipelines are respectively used for being connected with different solution containers in a one-to-one correspondence mode. The application provides a multi-functional liquid reaction unit can provide accurate, automatic, efficient experimental apparatus for the chemical detection experiment. The functions of accurate liquid feeding, participation of various liquids in reaction, constant-temperature heating (time and temperature are controllable and can be set), reaction, automatic treatment of experimental results and the like can be realized. Can realize the accurate quantitative automatic injection and automatic cleaning of various reagents. The use of the in-situ constant temperature heating technology and the PID constant temperature heating technology ensures that the temperature control is more accurate, and the efficiency and the accuracy can be improved. Is worthy of large-area popularization and application.
Of course, it is not necessary for any product in which the utility model is practiced to achieve all of the above-described advantages at the same time.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 is a schematic structural diagram of a multifunctional liquid reaction apparatus according to an embodiment of the present invention;
FIG. 2 is a circuit diagram of a first part of a multifunctional liquid reaction device according to an embodiment of the present invention;
FIG. 3 is a circuit diagram of a second part of a multifunctional liquid reaction device provided by an embodiment of the present invention;
FIG. 4 is a circuit diagram of a third part of a multifunctional liquid reaction device provided by an embodiment of the present invention;
FIG. 5 is a fourth part of a circuit diagram of a multifunctional liquid reaction device provided by an embodiment of the present invention;
FIG. 6 is a flow chart of a water permanganate index experiment performed by using the reaction device according to an embodiment of the present invention.
In the figure: the device comprises a passage switching unit 1, a public pipeline 11, a first branch pipeline 12, a second branch pipeline 13, an injection unit 2, a liquid storage unit 3, a reaction unit 4, a control unit 5, an audible and visual alarm component 51, a solution container 6, a pressure release valve 7, a light source 81, a photocell 82, a temperature control component 9 and a pure water valve 10.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments that can be derived by one of ordinary skill in the art from the embodiments given herein are intended to be within the scope of the present invention.
Examples
Referring to fig. 1, a multifunctional liquid reaction apparatus provided for an embodiment of the present invention, as shown in fig. 1, may include:
the device comprises a passage switching unit 1, an injection unit 2, a liquid storage unit 3, a reaction unit 4 and a control unit 5; the injection unit 2 is connected to the reservoir unit 3; the passage switching unit 1, the injection unit 2 and the reaction unit 4 are respectively in communication connection with the control unit 5;
the passage switching unit 1 comprises a common pipeline 11, a first branch pipeline 12 and a plurality of second branch pipelines 13; the liquid storage unit 3 is connected with the common pipeline 11, and the reaction unit 4 is connected with the first branch pipeline 12; the second branch pipelines 13 are respectively used for being connected with different solution containers 6 in a one-to-one correspondence manner;
the control unit 5 is configured to perform the following operations:
generating an experiment strategy according to a received experiment requirement, wherein the experiment strategy comprises a liquid suction strategy, a liquid injection strategy and a reaction strategy;
sending the imbibing strategy to the passage switching unit 1 and the injection unit 2 respectively, wherein the passage switching unit 1 is used for communicating the common pipeline 11 with a target second branch pipeline 13 according to the imbibing strategy, and the target second branch pipeline 13 is connected with a container of a target solution; the injection unit 2 is used for transferring the target solution to the liquid storage unit 3 after the common pipeline 11 is communicated with the target second branch pipeline 13;
the liquid injection strategy is respectively sent to the passage switching unit 1 and the injection unit 2, the passage switching unit 1 is used for communicating the common pipeline 11 with the first branch pipeline 12 according to the liquid injection strategy, and the injection unit 2 is used for transferring a target solution from the liquid storage unit 3 to the reaction unit 4 after the common pipeline 11 is communicated with the first branch pipeline 12;
and sending the reaction strategy to the reaction unit 4, so that the reaction unit 4 forms a target reaction environment inside the reaction unit 4 according to the reaction strategy.
The embodiment of the application provides a multi-functional liquid reaction unit can realize functions such as automatic feed liquor flow, automatic clear up, self-cleaning, automatic flowing back, result calculation through the control of the control unit. Can be as required automatic ration and/or add 2 or multiple reagent repeatedly and mix the back, or stand, or heat to the constant temperature, this reaction unit can be used to carry out the constant temperature heating of mixed liquid and clear up, quality of water permanganate survey experiment, quality of water ammonia nitrogen survey experiment, quality of water total phosphorus survey experiment etc..
In order to realize in-situ reaction after the solution enters the reaction unit, and reduce various adverse effects caused by solution transfer. The embodiment of the present application may provide that the reaction unit 4 includes an inlet end and an outlet end, and the inlet end and the outlet end are respectively configured with a pressure release valve 7; the pressure release valve 7 is communicably connected to the control unit 5; the control unit 5 is configured to send the reaction strategy to the pressure release valve 7, so that the pressure release valve 7 adjusts the on-off state according to the reaction strategy. Specifically, the reaction strategy comprises an injection starting instruction and an injection ending instruction; the pressure relief valve is used for adjusting to a conducting state after receiving the liquid injection starting instruction and adjusting to a blocking state after receiving the liquid injection finishing instruction. And the two ends of the reaction unit are respectively provided with a pressure relief valve which is respectively arranged at the upper end and the lower end of the reaction unit, when reagent is fed into the reaction unit, the upper pressure relief valve and the lower pressure relief valve are simultaneously opened, and after the reagent is injected, the upper pressure relief valve and the lower pressure relief valve are simultaneously closed, so that a closed space is formed in the reaction unit. And adjusting the internal environment of the closed space to a target reaction environment as required, and carrying out corresponding experimental reaction.
In order to automatically identify the concentration of the solution in the reaction unit, the embodiment of the application may further provide that the reaction unit includes a main body made of a transparent material, a photoelectric signal collecting assembly is configured on the main body, the photoelectric signal collecting assembly is communicably connected to the control unit 5, and the control unit 5 is configured to determine the concentration of the reagent in the main body according to a condition that the reagent collected by the photoelectric signal collecting assembly absorbs the light with the target wavelength. In order to eliminate the influence of external light on the collection precision of the optoelectronic signal collection assembly, the optoelectronic signal collection assembly may further include a light source 81 and a photocell 82 disposed on the main body, and the reaction unit 4 further includes a light shielding housing (not shown in the figure) disposed outside the main body.
The different colors of the solution are caused by the selective absorption of light of a certain color by the particles (molecules or ions) in the solution. If the light transmission degree of each color is the same, the substance is colorless and transparent. If only a portion of the wavelengths of light are transmitted and the other wavelengths are absorbed, the solution will appear the color of the transmitted light, i.e., the solution will appear the color that is complementary to the light it absorbs. For example, a copper sulfate solution is blue due to absorption of yellow light in white light; the potassium permanganate solution appears purple due to absorption of green light in the white light.
Therefore, the device provided by the embodiment of the application can judge the concentration of the reagent according to the capability (absorbance) of the reagent to absorb certain fixed wavelength light
The calculation of absorbance is based on Lambert beer's law
Lambert beer's law:
when a beam of parallel light vertically passes through a non-scattering light-absorbing substance, the Lambert beer law that the absorbance (A) of the beam of parallel light is in direct proportion to the concentration (c) of the light-absorbing substance and the optical path length (b) is the basic law of light absorption, the method is suitable for all electromagnetic radiation and all light-absorbing substances, reagents are changed by using different concentrations, the absorbance and concentration curves of the reagents are obtained, and the concentration of the reagents can be calculated according to the absorbance back-deduction.
If the device provided by the application is used for measuring the permanganate index concentration, calibration is needed firstly, if 4 concentration standard solutions are adopted, the concentrations are respectively 0mg/L, 5mg/L, 10mg/L and 20mg/L, the absorbance of the solution is obtained under the wavelength of 525nm after reaction, 4 absorbances are obtained,
then, a coordinate system is established by taking the absorbance as a horizontal axis and the concentration as a vertical axis, and the corresponding relation is calculated. The relation curve is divided into 3 sections, 0-5mg/L, 5-10 mg/L and 10-20mg/L, which correspond to k1, b1, k2, b2, k3, b3 and 6 parameters.
The photoelectric signal is collected by the control unit during calculation, 1 group of photoelectric signal data is recorded by upper computer software every 1 second when the experiment is finished, 15 groups of photoelectric signal data are recorded, and the absorbance is calculated after the average value is calculated. The 4 absorbances I1, I2, I3 and I4 are obtained, and then the known 0, 5, 10 and 20 are added to obtain three-segment straight lines of k1, b1, k2, b2, k3, b3 and 6 parameters. And finishing calibration.
If the absorbance of the instrument obtained in a certain experiment, I1< I2, then bringing I into the curve C-k 1I + b1 gives the modified solution permanganate index concentration.
Since different requirements are required for the temperature in the reaction unit when different experiments are performed, in order to realize automatic adjustment of the temperature in the reaction unit, the embodiment of the present application may provide that the reaction unit includes a temperature control component 9, the temperature control component 9 is communicably connected to the control unit 5, and the temperature control component 9 is configured to adjust the temperature in the reaction unit 4 according to the reaction strategy so as to form a target reaction environment inside the reaction unit 4. The temperature control assembly 9 may include a heating wire thermistor, a temperature sensor, a heat radiation fan, and the like. The heating wire thermistor is used for realizing heating, the temperature sensor is used for detecting the temperature in the reaction unit in real time, and the cooling fan can cool the inside of the reaction unit as required. The temperature control assembly can ensure that the reaction unit is always at the optimal reaction temperature, thereby achieving the effects of improving the reaction efficiency, the reaction quality and the like.
In practical application, the type of the channel switching unit can be selected as required, for example, in one implementation, the embodiment of the present application may provide that the channel switching unit 1 includes a multi-channel switching valve island including a switching power assembly, and the multi-channel switching valve island includes a sapphire valve core and a valve head having a polytrifluoroethylene corrosion-resistant layer. The valve hole positioning of the multi-channel switching valve island adopts a coded disc optocoupler mode for positioning, and the switching power assembly comprises a planetary reduction gearbox motor.
The multi-channel switching valve island provided by the embodiment of the application is internally integrated with a motor control circuit, and the operation of the stepping motor is controlled by receiving an instruction sent by a control unit CPU, so that the switching of a fluid channel is realized. The switching valve adopts a sapphire valve core, can be suitable for various corrosive liquids, and is free of maintenance due to the fact that the valve head is formed by machining and combining PCTFE (polytrifluoroethylene) and 316 stainless steel. And the liquid contact surface is not in contact with the stainless steel. The valve core adopts a multidirectional self-adaptive plane laminating mode, so that the service life of a product can be effectively prolonged. The valve body rotates by adopting an NMB inlet planetary reduction gearbox motor as a power device, and the reliability is extremely high. The valve hole is positioned by adopting a coded disc optocoupler, so that the problem that the reduction gearbox cannot be accurately positioned after being worn can be effectively solved; the driving module adopts a low-power consumption driving chip, and the influence of heat generated during the operation of the chip on the performance can be effectively reduced. The performance indexes of the multi-channel switching valve island provided by the embodiment of the application are shown in table 1:
TABLE 1
In order to improve the liquid injection precision during automatic liquid injection, the embodiment of the present application may further provide that the injection unit 2 includes a vertical injection pump; the vertical injection pump is respectively connected with the liquid storage unit 3 and a pure water supply unit comprising a pure water valve 10; the liquid storage unit comprises a liquid storage ring. The vertical injection pump can provide the functions of accurately sucking and injecting reagents. The performance index of the vertical injection pump provided by the embodiment of the application is shown in table 2.
TABLE 2
In order to realize accurate control of each unit, the embodiment of the application may further provide that the control unit includes an STM32F373CBT6 single chip microcomputer, and the control unit is further connected with an audible and visual alarm component.
Referring to fig. 2, fig. 3, fig. 4, and fig. 5, U5 is an STM32F373CBT6 single chip microcomputer, and as a main control chip of a lower computer of the whole embedded hardware system, an upper computer reset circuit formed by R16 and C13 and an X1(8MHz active crystal oscillator) form a minimum system, so that the single chip microcomputer can work normally.
Photo sen is a photosensitive device, and U7(OPA2156IDR) and U8(OPA2192ID) are high-precision operational amplifiers. When the photosensitive device senses light with a central wavelength of a specific frequency, the photosensitive device converts light intensity into micro current, the micro current is converted into voltage through R23, the voltage is amplified and conditioned through a circuit formed by U7 and U8, and then the voltage is output to the analog voltage acquisition port of the single chip microcomputer. Wherein, R24, D18(BAT54S) constitute voltage clamp, current limiting circuit, can protect the safety of the analog voltage acquisition port of singlechip.
U11 is OPA2192ID operational amplifier, NTC100K thermistor and R31 form the voltage division type temperature detection circuit, pass the voltage follower of U11A, R43 current-limiting resistance, transmit to STM32F373C8T6 analog signal acquisition input end; after passing through an STM32F373C8T6 internal algorithm, the voltage value is converted into an actual temperature value. The heating unit core device is high-power MOS pipe IRF3205ZS, the temperature that the temperature loop was gathered with NTC100K is as the input, take 2 KHz's PWM duty cycle as output, the singlechip passes through the break-make of the PWM switching frequency control MIOS pipe of pin 2KHz, the heating wire heating of drive, form the temperature closed loop with NTC100K, through STM32F373C8T6 inside PID algorithm realization accurate constant temperature control, provide accurate temperature environment for chemical reaction.
U10, U9 two K78U05 (3-end voltage stabilization chip) form a double-track power supply to supply power to the operational amplifier, and C35, C36, C37 and C39 filter the output voltage, so that the output voltage is more stable;
u1(LM2596), U2(LT1117) constitute power module, convert 24V power into 5V, 3.3V and supply power to singlechip and other peripheral components.
U3(SP3485) is TTL to 485 communication chips, and realizes the communication between the main control board and the upper computer. D8, D9, D11 are TVS diodes, can protect the communication interface effectively, avoid external interference.
U6(SP3232EEN) is serial port communication chip, and collocation C11, C12, C14, C15 constitute serial port communication unit, contain 2 way serial ports, communicate with vertical syringe pump, multichannel switching valve guide respectively, therefore STM32F373C8T6 can send serial port instruction through this serial port unit, control vertical syringe pump, multichannel switching valve guide, realize automatic feed liquor function.
U4(MB85RC16PNF-G-JNE1) is an external storage chip, so that data can be stored without losing due to power failure.
The switch circuit composed of Q1, Q5, Q10 and Q12(MOS tubes) respectively controls the electromagnetic valves of pure water, pressure relief, heating and heat dissipation.
D16 (light emitting diode), D17 (light emitting diode), LS1 (buzzer) constitute the acousto-optic warning circuit, when abnormal conditions occur, the singlechip carries out the warning.
The acousto-optic alarm assembly provided by the embodiment of the application can comprise a buzzer alarm and a red LED alarm. When the reagent is changed, the LED emits a 'tic' sound to prompt the operator to finish the process.
When the water sample is changed, the LED emits a 'tic' sound to prompt the operator to finish the change.
When the cleaning action is completed, the LED emits a 'tic' to prompt the operator to complete.
When the heat dissipation is finished, the LED emits a click to prompt the operator to finish the operation.
When the heat dissipation is finished, the LED emits a click to prompt the operator to finish the process.
When the digestion is completed, the LED emits a 'tic' sound to prompt the operator to complete.
When the shut down resolution is complete, the LED emits a "beep" to alert the operator to completion.
When the temperature of the digestion tank is too high, the LED sends out alarm sound, and meanwhile, the LED lights up.
The utility model can be understood as a set of proposal of the utility model, and in order to illustrate the effect brought by the method of the utility model, the proposal is used for measuring by taking a water quality permanganate index measuring experiment as an example.
1. The injection pump and the valve guide used in the device have the function of being controlled through serial port instructions, so that the device has high automation degree, can realize full-automatic liquid feeding, cleaning, liquid discharging, bubbling and other functions through cpu instructions, and greatly improves the working efficiency.
Common serial port instructions:
valve guide reset (except check code): 0xCC,0x02,0x45,0x00,0x00,0 xDD;
valve guide selection channel 1 detection pool pipeline (except check code): 0xCC,0x02,0x44,0x01,0x00,0 xDD;
valve guide selection channel 2 waste liquid pool pipeline (except check code): 0xCC,0x02,0x44,0x02,0x00,0 xDD;
valve guide selection channel 3 reserves the pipeline (except check code): 0xCC,0x02,0x44,0x03,0x00,0 xDD;
valve guide selection channel 4 water sample pipeline to be tested (except check code): 0xCC,0x02,0x44,0x04,0x00,0 xDD;
the valve guide selection channel 5 is reserved with a pipeline (except for a check code): 0xCC,0x02,0x44,0x05,0x00,0 xDD;
valve guide selects 6 potassium permanganate solution pipelines of passageway (except check code): 0xCC,0x02,0x44,0x06,0x00,0 xDD;
valve guide selection channel 7 reserves the pipeline (except check code): 0xCC,0x02,0x44,0x07,0x00,0 xDD;
valve guide selection channel 8 reserves the pipeline (except check code): 0xCC,0x02,0x44,0x08,0x00,0 xDD;
valve guide selection channel 9(1+3) sulfuric acid pipeline (except check code): 0xCC,0x02,0x44,0x09,0x00,0 xDD;
valve guide selection channel 10 air line (except check code): 0xCC,0x02,0x44,0x0A,0x00,0 xDD;
syringe pump advance 0xAABB step number command (divide check code): 0xCC,0x01,0x41,0xBB,0xAA,0 xDD;
syringe pump back 0xAABB step number command (divide check code): 0xCC,0x01,0x42,0xBB,0xAA,0 xDD;
syringe pump reset (except check code): 0xCC,0x01,0x45,0x00,0x00,0 xDD;
2. the injection pump of high accuracy, minimum feed liquor precision is 0.0025 mm/1.0381 mu L, has guaranteed the precision of feed liquor volume, cooperates the multichannel switching valve to lead for the order of reagent in the experiment is injected, is discharged, more nimble, high-efficient.
3. The in-situ heating technology ensures that the reagent is safer and more convenient in the heating process, and can be heated at constant temperature according to the set temperature and time without moving the position.
To illustrate the practical effects of the device provided in the present application, as shown in fig. 6, a water permanganate index experiment is taken as an example to describe how the device can realize a fully automatic workflow in such an experiment:
step 1: the method comprises the following steps of instrument power-on, system initialization, syringe pump resetting (serial commands: 0xCC,0x01,0x45,0x00,0x00 and 0xDD), valve guide resetting (serial commands: 0xCC,0x02,0x45,0x00,0x00 and 0xDD), clear water valve opening, syringe pump advancing 4815 steps (serial commands: 0xCC,0x01,0x41,0xCF,0x12 and 0xDD), syringe pump resetting (serial commands: 0xCC,0x01,0x45,0x00 and 0x00 and 0xDD), valve guide switching to a waste liquid pool pipeline (serial commands: 0xCC,0x02,0x44,0x02,0x00 and 0xDD), syringe pump resetting (serial commands: 0xCC,0x01,0x45,0x00 and 0x xDD), and pumping out 5mL of deionized water to a liquid loop to fill the liquid in a liquid storage loop.
Step 2: liquid drainage action: the valve is switched to a detection pool pipeline (0xCC,0x02,0x44,0x01,0x00 and 0xDD), a pressure relief valve is opened, the injection pump advances 3274 steps (serial port commands: 0xCC,0x01,0x41,0xCA,0x0C and 0xDD), 3.4mL of waste liquid is extracted, the pressure relief valve is closed, the valve is switched to a waste liquid pool pipeline (serial port commands: 0xCC,0x02,0x44,0x02,0x00 and 0xDD), the injection pump is reset (serial port commands: 0xCC,0x01,0x45,0x00,0x00 and 0xDD), and the waste liquid is injected into the waste liquid pool. The liquid discharge action is circulated for 3 times, and the waste liquid in the waste liquid pool is emptied. The system initialization is complete.
And 3, step 3: (1+3) sulfuric acid injection: valve guide resetting (serial commands: 0xCC,0x02,0x45,0x00,0x00 and 0xDD), opening a clear water valve, advancing the injection pump by 4815 steps (serial commands: 0xCC,0x01,0x41,0xCF,0x12 and 0xDD), sucking 5ml of deionized water, closing the clear water valve, switching the valve guide to a waste liquid pool pipeline (serial commands: 0xCC,0x02,0x44,0x02,0x00 and 0xDD), resetting the injection pump (serial commands: 0xCC,0x01,0x45,0x00,0x00 and 0xDD), and filling the liquid storage ring with deionized water. The valve guide is switched to a (1+3) sulfuric acid pipeline (serial port instruction 0xCC,0x02,0x44,0x09,0x00,0xDD), the injection pump advances 2408 steps (serial port instruction: 0xCC,0x01,0x41,0x68,0x09,0xDD), 2.5mL (1+3) sulfuric acid is sucked, the valve guide is switched to a waste liquid pool pipeline (serial port instruction: 0xCC,0x xDD, 0x xDD, 0x xDD, 0xDD), the injection pump retreats 193 steps (serial port instruction: 0xCC,0x xDD, 0x xDD, 0xC xDD, 0x xDD, 0x xDD, 0xDD), the injection pump injects 0.2mL (1+3) sulfuric acid into the waste liquid pool, the valve guide is switched to a detection pool pipeline (serial port instruction: 0xCC,0x xDD, 0x xDD, 0x xDD, 0x xDD, 0x xDD, the injection pump retreats in the detection pool pipeline (serial port instruction: 0x xDD, step C) is switched to a step C, xDD, the injection pump is injected, the detection pool pipeline (serial port instruction: xDD, xDD step is injected, xDD, the injection pump is injected, the step of the injection pump is switched to a step is injected, the detection step of the waste liquid pool, the injection pump is switched to a step of the detection pool, the step of the detection step of the injection pump is carried out, 0x02,0x44,0x02,0x00,0xDD), syringe pump reset (serial command: 0xCC,0x01,0x45,0x00,0x00,0xDD), 0.3mL of the remaining (1+3) sulfuric acid is injected into the waste liquid pool. The valve guide is switched to an air pipeline (serial port instruction: 0xCC,0x02,0x44,0x0A,0x00,0xDD), the injection pump advances 482 steps (serial port instruction: 0xCC,0x01,0x41,0xE2,0x01,0xDD), 0.5mL of air is sucked, the valve guide is switched to a (1+3) sulfuric acid pipeline (serial port instruction: 0xCC,0x xDD, 0x xDD, 0x xDD, 0xDD), the injection pump retreats 96 steps (serial port instruction: 0xCC,0x xDD, 0x xDD, 0x xDD, 0xDD), 0.1mL of air is injected to the (1+3) sulfuric acid pipeline, the valve guide is switched to a liquid pool pipeline (serial port instruction: 0xCC,0x xDD, 0x xDD, 0x xDD, 0x xDD, 6850 x xDD, a waste pump is reset instruction: 0x xDD, a waste liquid pool (serial port instruction: 0x xDD, 0x xDD, 0x xDD) of air and a waste liquid pool, xDD, and a waste pump is injected.
And 4, step 4: selectively injecting a potassium permanganate solution: valve guide resetting (serial commands: 0xCC,0x02,0x45,0x00,0x00 and 0xDD), opening a clear water valve, advancing the injection pump by 4815 steps (serial commands: 0xCC,0x01,0x41,0x12,0xCF,0xDD), sucking 5ml of deionized water, closing the clear water valve, switching the valve guide to a waste liquid pool pipeline (serial commands: 0xCC,0x02,0x44,0x02,0x00 and 0xDD), resetting the injection pump (serial commands: 0xCC,0x01,0x45,0x00,0x00 and 0xDD), and filling the liquid storage ring with deionized water. The valve guide is switched to a potassium permanganate solution pipeline (serial port instruction: 0xCC,0x02,0x44,0x06,0x00,0xDD), the injection pump advances 2408 steps (serial port instruction: 0xCC,0x01,0x41,0x68,0x09,0xDD), 2.5mL potassium permanganate solution is sucked, the valve guide is switched to a waste liquid pool pipeline (serial port instruction: 0xCC,0x xDD, 0x xDD, 0x xDD, 0xDD), the injection pump retreats step (serial port instruction: 0xCC,0x xDD, 0x xDD, 0x xDD, 0xC xDD, 0x xDD, 0x xDD) and the injection pump retreats to a waste liquid pool pipeline (serial port instruction: 0xCC,0x xDD, 0x xDD, 0x xDD, 0x xDD, xDD) and the valve guide is switched to a detection pool pipeline (serial port instruction: 0xCC,0x xDD, the injection pump retreats step is switched to a waste liquid pool pipeline (serial port instruction: xDD, the injection pump retreats step is injected into a serial port instruction after the injection pump retreats step of the injection pump, the injection pump retreats step (serial port instruction: 0x xDD, xDD step of the valve guide is switched to a serial port instruction: 0x xDD, the injection pump retreats step of the injection pump, the injection step of the injection pump is switched to a serial port instruction: 0x xDD, the injection step of the injection pump is switched to a serial port instruction: 0x xDD, the injection pump is switched to a step of the injection step of the sample is switched to a serial port instruction: 0x xDD, and the sample is switched to a serial port of the sample is switched to a (serial port of the sample is switched to a serial port instruction: 0x xDD, 0x xDD, and the sample is switched to a step of the sample is switched to a serial port of the sample is switched to a serial port of the sample is switched to the sample pool (serial port of the sample is fluid sample of the sample is of the sample pool (serial port of, 0x02,0x44,0x02,0x00,0xDD), syringe pump reset (serial command: 0xCC,0x01,0x45,0x00,0x00,0xDD), and the residual potassium permanganate solution is sent to a waste liquid pool. The valve guide is switched to an air pipeline (serial port instruction: 0xCC,0x02,0x44,0x0A,0x00,0xDD), the injection pump advances 482 steps (serial port instruction: 0xCC,0x01,0x41,0xE2,0x01,0xDD), 0.5mL of air is sucked, the valve guide is switched to a potassium permanganate solution pipeline (serial port instruction: 0xCC,0x xDD, 0x xDD, 0x xDD, 0xDD), the injection pump retreats 96 steps (serial port instruction: 0xCC,0x xDD, 0x xDD, 0x xDD, xDD x xDD, 0x xDD, the injection pump is reset (serial port instruction: 0x xDD, 0x xDD, xDD x) and the waste liquid pool pipeline is switched to the valve guide.
And 5, step 5: selectively filling a water sample to be detected: the method comprises the following steps of valve guide resetting (serial commands: 0xCC,0x02,0x45,0x00,0x00 and 0xDD), opening a purified water valve, advancing the injection pump by 4815 steps (serial commands: 0xCC,0x01,0x41,0x12,0xCF,0xDD), sucking 5ml of deionized water (serial commands: 0xCC,0x01,0x41,0xCF,0x12 and 0xDD), closing the purified water valve, switching the valve guide to a waste liquid pool pipeline (serial commands: 0xCC,0x02,0x44,0x02,0x00 and 0xDD), and injection pump resetting (serial commands: 0xCC,0x01,0x45,0x00,0x00 and 0xDD), and filling a liquid storage ring with deionized water. The valve guide is switched to a water sample pipeline to be detected (serial port instruction: 0xCC,0x02,0x44,0x04,0x00 and 0xDD), the injection pump advances (serial port instruction: 0xCC,0x01,0x41,0x68,0x09 and 0xDD), 2.5mL of water sample to be detected is sucked, the valve guide is switched to a waste liquid pool pipeline (serial port instruction: 0xCC,0x02,0x44,0x02,0x00 and 0x 0xDD), the injection pump retreats in 193 steps, (serial port instruction: 0xCC,0x01,0x42,0xC1,0x00 and 0xDD) and the valve guide is switched to a detection pool pipeline (serial port instruction: 0xCC,0x02,0x44,0x01,0x00 and 0x0 xDD).
The injection pump advances 1926 (serial port instruction: 0xCC,0x01,0x41,0x86,0x07 and 0xDD), 2mL of water sample to be detected is injected into the detection pool, the valve guide is switched to the waste liquid pool pipeline (serial port instruction: 0xCC,0x02,0x44,0x02,0x00 and 0xDD), the injection pump is reset (serial port instruction: 0xCC,0x01,0x45,0x00,0x00 and 0xDD), and 0.3mL of residual water sample to be detected is injected into the waste liquid pool. The valve guide is switched to an air pipeline (serial port instruction: 0xCC,0x02,0x44,0x0A,0x00 and 0xDD), the injection pump advances by 482 steps (serial port instruction: 0xCC,0x01,0x41,0xE2,0x01 and 0xDD) and sucks 0.5mL of air, and the valve guide is switched to a water sample pipeline to be detected (serial port instruction: 0xCC,0x02,0x44,0x04,0x00 and 0 xDD).
And (3) retreating the injection pump for 96 steps, injecting 0.1mL of air into a water sample pipeline to be detected, switching the valve guide to a waste liquid pool pipeline (serial port instructions: 0xCC,0x02,0x44,0x02,0x00 and 0xDD), resetting the injection pump (0xCC,0x01,0x45,0x00,0x00 and 0xDD), and sending the residual air to the waste liquid pool pipeline. And (5) completing reagent filling.
And 6, step 6: the heating was turned on and digestion time was recorded when the temperature reached the boiling water bath temperature.
And 7, step 7: when the digestion time reached 30 minutes, the absorbance was measured under visible light of 525nm wavelength.
And 8, step 8: liquid drainage action: the valve guide is switched to a detection pool pipeline (serial port instruction: 0xCC,0x02,0x44,0x01,0x00 and 0xDD), a pressure relief valve is opened, the injection pump advances 3274 steps (serial port instruction: 0xCC,0x01,0x41,0xCA,0x0C and 0xDD), 3.4mL of waste liquid is extracted, the pressure relief valve is closed, the valve guide is switched to a waste liquid pool pipeline (serial port instruction: 0xCC,0x02,0x44,0x02,0x00 and 0xDD), the injection pump is reset (serial port instruction: 0xCC,0x01,0x45,0x00,0x00 and 0xDD), and the waste liquid is injected into a waste liquid pool. The liquid discharge action is circulated for 3 times, and the waste liquid in the waste liquid pool is emptied.
Step 9: opening a purified water valve, advancing the injection pump to 8186 steps (serial commands: 0xCC,0x01,0x41,0xFA,0x1F and 0xDD), drawing 8.5mL of deionized water, closing a water inlet valve, switching a valve guide to a waste liquid pool pipeline (serial commands: 0xCC,0x02,0x44,0x02,0x00 and 0xDD), pumping 0.2mL of deionized water to the waste liquid pool pipeline by the injection pump, switching the valve guide to a detection pool pipeline (serial commands: 0xCC,0x02,0x44,0x01,0x00 and 0xDD), and retreating the injection pump to 7704 steps (serial commands: 0xCC,0x01,0x42,0xBB,0xAA and 0xDD), injecting 8mL of deionized water to the reaction pool, the valve guide was switched to the waste reservoir line (serial commands: 0xCC,0x02,0x44,0x02,0x00,0xDD), the syringe pump was reset (serial commands: 0xCC,0x01,0x45,0x00,0x00,0xDD), and the remaining 0.3mL of deionized water was injected into the waste reservoir.
Step 10: the absorbance was measured under visible light of 525nm wavelength.
And 11, step 11: liquid drainage action: the valve guide is switched to a detection pool pipeline (serial port instruction: 0xCC,0x02,0x44,0x01,0x00 and 0xDD), a pressure relief valve is opened, the injection pump advances 3274 steps (serial port instruction: 0xCC,0x01,0x41,0xCA,0x0C and 0xDD), 3.4mL of waste liquid is extracted, the pressure relief valve is closed, the valve guide is switched to a waste liquid pool pipeline (serial port instruction: 0xCC,0x02,0x44,0x02,0x00 and 0xDD), the injection pump is reset (serial port instruction: 0xCC,0x01,0x45,0x00,0x00 and 0xDD), and the waste liquid is injected into a waste liquid pool. The liquid discharge action is circulated for 3 times, and the waste liquid in the waste liquid pool is emptied. And finishing the reagent filling process.
In this whole process, include
(1) An automatic sequential liquid inlet process of a potassium permanganate solution, a water sample to be detected and 1+3 sulfuric acid;
(2) high-temperature water bath and digestion process of 30 minutes;
(3) automatically collecting a photoelectric limit number when the digestion is finished, and recording the process;
(4) the automatic cooling process after digestion is finished;
(5) automatic liquid drainage process;
(6) automatic sample zero-feeding, cleaning, comparing and digesting processes;
(7) and (5) zero sample liquid discharging process.
The whole process operation is operated by an upper computer, the whole process is highly automated, and each decomposition action has a corresponding menu operation function and automatic data recording and analysis.
In a word, the multi-functional liquid reaction unit that this application provided can provide accurate, automatic, efficient experimental apparatus for the chemical detection experiment. The functions of accurate liquid feeding, participation of various liquids in reaction, constant-temperature heating (time and temperature are controllable and can be set), reaction, automatic treatment of experimental results and the like can be realized. Can realize the accurate quantitative automatic injection and automatic cleaning of various reagents. The use of the in-situ constant temperature heating technology and the PID constant temperature heating technology ensures that the temperature control is more accurate, and the efficiency and the accuracy can be improved. Is worthy of large-area popularization and application.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
The above description is only for the preferred embodiment of the present invention, and is not intended to limit the scope of the present invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention shall fall within the protection scope of the present invention.
Claims (9)
1. A multifunctional liquid reaction device is characterized by comprising a passage switching unit, an injection unit, a liquid storage unit, a reaction unit and a control unit; the injection unit is connected to the reservoir unit; the passage switching unit, the injection unit and the reaction unit are respectively in communication connection with the control unit;
the passage switching unit comprises a common pipeline, a first branch pipeline and a plurality of second branch pipelines; the liquid storage unit is connected with the public pipeline, and the reaction unit is connected with the first branch pipeline; the second branch pipelines are respectively used for being connected with different solution containers in a one-to-one correspondence mode.
2. The multifunctional liquid reaction device according to claim 1, wherein the reaction unit comprises an inlet end and an outlet end, and the inlet end and the outlet end are respectively provided with a pressure relief valve; the pressure relief valve is in communication connection with the control unit; the control unit is used for sending the reaction strategy to the pressure release valve so that the pressure release valve can adjust the on-off state according to the reaction strategy.
3. The multifunctional liquid reaction device of claim 1, wherein the reaction unit comprises a main body made of transparent material, the main body is provided with an optoelectronic signal acquisition assembly, the optoelectronic signal acquisition assembly is in communication connection with the control unit, and the control unit is used for judging the concentration of the reagent in the main body according to the condition that the reagent acquired by the optoelectronic signal acquisition assembly absorbs the light with the target wavelength.
4. The multifunctional liquid reaction device as claimed in claim 3, wherein the photoelectric signal collecting assembly comprises a light source and a photoelectric cell oppositely disposed on the main body, and the reaction unit further comprises a light shielding case disposed outside the main body.
5. The multi-functional liquid reaction device of claim 1, wherein the reaction unit comprises a temperature control assembly communicatively coupled to the control unit, the temperature control assembly configured to adjust a temperature within the reaction unit according to a reaction strategy to create a target reaction environment within the reaction unit.
6. The multifunctional liquid reaction device according to claim 1, wherein the path switching unit comprises a multi-path switching valve island including a switching power component, the multi-path switching valve island comprising a sapphire spool and a valve head having a polytrifluoroethylene corrosion-resistant layer.
7. The multifunctional liquid reaction device as claimed in claim 6, wherein the valve hole of the multi-channel switching valve island is positioned by using a code wheel optical coupler, and the switching power assembly comprises a planetary reduction gearbox motor.
8. The multifunctional liquid reaction device according to claim 1, wherein the injection unit comprises a vertical injection pump; the vertical injection pump is respectively connected with the liquid storage unit and the pure water supply unit comprising a pure water valve; the liquid storage unit comprises a liquid storage ring.
9. The multifunctional liquid reaction device as claimed in claim 1, wherein the control unit comprises an STM32F373CBT6 single chip microcomputer, and the control unit is further connected with an audible and visual alarm component.
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