CN216378222U - Biochemical reaction testing device for BOD strains - Google Patents
Biochemical reaction testing device for BOD strains Download PDFInfo
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- CN216378222U CN216378222U CN202122561674.7U CN202122561674U CN216378222U CN 216378222 U CN216378222 U CN 216378222U CN 202122561674 U CN202122561674 U CN 202122561674U CN 216378222 U CN216378222 U CN 216378222U
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Abstract
The utility model provides a biochemical reaction testing device for BOD strains, which comprises a sample liquid bottle, a first driving element, a multi-way valve, a plurality of strain reactors and a plurality of DO detectors corresponding to the strain reactors, wherein strains contained in each strain reactor are different; the sample liquid bottle, the first driving element, the multi-way valve, the strain reactor and the DO detector are communicated in sequence through pipelines; the sample liquid is driven by the first driving element to flow into different strain reactors through multi-way valves respectively for biochemical reaction, and finally, a DO detector is used for carrying out multi-index simultaneous detection on the reaction liquid. The utility model is a dissolved oxygen test after the reaction of multi-channel sample liquid and different strains, can realize the simultaneous detection of a plurality of indexes by one sample, and increases the stability and the accuracy of the detection result.
Description
Technical Field
The utility model relates to the technical field of environmental monitoring equipment, in particular to a biochemical reaction testing device for BOD strains.
Background
BOD is the amount of dissolved oxygen in water consumed in the process of decomposing organic substances by microorganisms in water, is an important monitoring item in water quality environment monitoring as a comprehensive index for representing the content of organic pollutants in water, has higher value and more serious surface pollution, and has been widely used for water quality monitoring of natural water and operation control of sewage treatment plants.
The key core of BOD online monitoring is closely related to the dissolved oxygen state in water and related to the survival conditions of different microbial strains in water, certain correlations exist between the BOD and the types, activities and the like of the microbial strains under specific conditions, and the conventional BOD online monitoring equipment is not combined with the diversity and activity indexes of the microbial strains, can only detect a single index, seriously influences the precision and stability of a monitoring result and reduces the monitoring efficiency. The utility model carries out multi-index detection through multi-channel strain reaction, comprehensively monitors and analyzes the consumption condition of the dissolved oxygen in the water, and improves the efficiency and the accuracy of BOD on-line monitoring.
SUMMERY OF THE UTILITY MODEL
In view of the above, the utility model provides a biochemical reaction testing device for BOD strains, which is used for multi-channel strain reaction and comprehensive detection of multiple indexes.
The technical scheme of the utility model is realized as follows: the utility model provides a biochemical reaction testing device for BOD strains, which comprises a sample liquid bottle, a first driving element, a multi-way valve, a plurality of strain reactors and a plurality of DO detectors corresponding to the strain reactors, wherein strains contained in each strain reactor are different; the sample liquid bottle, the first driving element, the multi-way valve, the strain reactor and the DO detector are communicated in sequence through pipelines; the sample liquid is driven by the first driving element to flow into different strain reactors through multi-way valves respectively for biochemical reaction, and finally, a DO detector is used for carrying out multi-index simultaneous detection on the reaction liquid.
On the basis of the technical scheme, the strain reactor preferably further comprises a plurality of second driving elements, and each strain reactor is communicated with the multi-way valve through one second driving element.
On the basis of the above technical scheme, preferably, there are three second driving elements, three strain reactors and three DO detectors; the strain reactors are a nitrifying bacteria strain reactor, a denitrifying bacteria strain reactor and a bacillus strain reactor respectively.
On the basis of the technical scheme, the automatic strain-culturing device preferably further comprises a constant-temperature bin for controlling the temperature of strain reaction, and the multi-way valve, the second driving element, the strain reactor and the DO detector are all located inside the constant-temperature bin.
On the basis of the technical scheme, the liquid sampler preferably further comprises a buffer liquid bottle and a third driving element, wherein the buffer liquid bottle, the third driving element and the sample liquid bottle are sequentially connected through a pipeline; the buffer solution flows into the sample solution bottle under the driving of the third driving element and is mixed with the sample solution.
On the basis of the technical scheme, preferably, the buffer liquid bottle is connected with the multi-way valve through a pipeline, and a fourth driving element is arranged between the buffer liquid bottle and the multi-way valve.
On the basis of the above technical solution, preferably, the first driving element, the second driving element, the third driving element and the fourth driving element are peristaltic pumps, and flow meters are disposed thereon.
On the basis of the technical scheme, the DO detector preferably further comprises a plurality of waste liquid bottles, and a liquid outlet of each DO detector is connected with one waste liquid bottle.
On the basis of the technical scheme, preferably, the strain reactor is spiral.
On the basis of the technical scheme, preferably, the liquid outlet of the buffer liquid bottle is connected with a valve of the multi-way valve.
On the basis of the technical scheme, the multi-channel strain reaction device is preferred to further comprise a constant temperature bin, wherein the constant temperature bin is installed at the bottom of the multi-channel strain reaction component and used for carrying out temperature control on strain reaction, and the multi-channel strain reaction component is located in the constant temperature bin.
Compared with the prior art, the biochemical reaction testing device for the BOD strain has the following beneficial effects:
(1) the utility model is a dissolved oxygen test after the reaction of multi-channel sample liquid and different strains, can realize the simultaneous detection of a plurality of indexes of one sample, and increases the stability and the accuracy of the detection result; the mixing ratio and the flow speed of the sample liquid and the buffer liquid are adjusted by a multi-way valve and a sample liquid peristaltic pump.
(2) The strain reactors are multiple, different strains can be added according to detection indexes, and the strains are not limited to the strains mentioned in the embodiment; the multi-way valve can select which seed reaction vessels the solution flows through.
(3) The liquid inlet of the DO detector is lower than the liquid outlet, the liquid inlet is connected with the strain reactor, the solution continuously flows in from the water inlet and can be automatically discharged from the water outlet after reaching a certain height water level, the detection time of a detected sample is prolonged, and the accuracy of the result is improved.
(4) The utility model is mainly used for BOD online monitoring work, carries out BOD monitoring instrument principle verification and improves the BOD online monitoring efficiency and accuracy of the instrument.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a front view of the biochemical reaction testing apparatus for BOD bacterial species according to the present invention;
FIG. 2 is a cross-sectional view of a front view of the biochemical reaction testing apparatus for BOD bacterial species according to the present invention;
FIG. 3 is a schematic structural diagram of the biochemical reaction testing device for BOD strains according to the present invention.
In the figure, a 1-sample liquid bottle, a 2-first driving element, a 3-multi-way valve, a 4-strain reactor, a 5-DO detector, a 6-second driving element, a 7-constant temperature bin, an 8-buffer liquid bottle, a 9-third driving element, a 10-fourth driving element, an 11-flowmeter and a 12-waste liquid bottle.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
As shown in fig. 1, with reference to fig. 2-3, the biochemical reaction testing device for BOD strains according to the present invention comprises a sample liquid bottle 1, a first driving element 2, a multi-way valve 3, a plurality of strain reactors 4 and a plurality of DO detectors 5 corresponding to the strain reactors, wherein each strain reactor 4 contains different strains; the sample liquid bottle 1, the first driving element 2, the multi-way valve 3, the strain reactor 4 and the DO detector 5 are communicated in sequence through pipelines; the sample liquid is driven by the first driving element 2 to flow into different strain reactors 4 respectively through the multi-way valve 3 for biochemical reaction, and finally, the DO detector 5 is used for carrying out multi-index simultaneous detection on the reaction liquid.
The sample liquid bottle 1 is used for containing sample liquid to be detected, and the sample liquid is water in a reservoir, a lake or a river to be detected; the multi-way valve comprises a plurality of valves and is used for connecting a plurality of devices; the first driving element is used for conveying the sample liquid from the sample liquid bottle to the multi-way valve and providing power for the flow of the sample liquid; a strain reactor, a container for reacting sample liquid with strains, and a microorganism strain contained in the container; and the DO detector is used for conveying the sample liquid to the DO detector after the sample liquid reacts with the strain, and detecting the reaction liquid to judge the components and the concentration of the pollutants contained in the sample liquid.
In the specific embodiment, a plurality of second driving elements 6 are further included, and each strain reactor 4 is communicated with the multi-way valve 3 through one second driving element 6. The second driving element provides power for the sample to be tested to flow to the strain reactor, and the normal reaction is ensured.
In a specific embodiment, a corresponding strain reactor is arranged according to the number of indexes to be detected of a sample to be detected, and the selectable microbial strains of the strain reactor are nitrifying bacteria: nitrosomonas, nitrosospira, nitrosococcus; denitrifying bacteria: denitrifying bacteria, Bacillus stewartii, and Xenorhabdus species; compound sewage treatment strain: bacillus and Pseudomonas; COD degrading strain. The multi-way valve can switch the sample liquid to be detected to enter which strain reactors through the closing or opening of the valve, and which indexes are detected.
Preferably, there are three of the second drive element 6, the seed reactor 4 and the DO detector 5.
Preferably, the strain reactors are a nitrifying bacteria strain reactor, a denitrifying bacteria strain reactor and a bacillus strain reactor respectively.
In a specific embodiment, in order to control the strain reaction temperature, a constant temperature chamber is further included, and the multi-way valve 3, the second driving element 6, the strain reactor 4 and the DO detector 5 are all located inside the constant temperature chamber 7.
Specifically, in order to achieve better temperature control, the constant-temperature cabin adopts water bath temperature control.
In a specific embodiment, the sample liquid sampling device further comprises a buffer liquid bottle 8 and a third driving element 9, wherein the buffer liquid bottle 8, the third driving element 9 and the sample liquid bottle 1 are sequentially connected through a pipeline; the buffer solution flows into the sample solution bottle 1 under the driving of the third driving element 9, and is mixed with the sample solution. The buffer solution bottle 8 is used for containing PBS buffer solution required by strain biochemical reaction, and the third driving element not only provides power for the flowing of the buffer solution, but also can control the proportion of the buffer solution and the sample solution.
In a specific embodiment, the buffer liquid bottle 8 is connected with the multi-way valve 3 through a pipeline, and a fourth driving element 10 is arranged between the buffer liquid bottle 8 and the multi-way valve 3.
In a specific embodiment, the first, second, third and fourth driving elements 2, 6, 9 and 10 are peristaltic pumps, on which a flow meter 11 is arranged for controlling the solution flow rate.
Preferably, the device also comprises a plurality of waste liquid bottles 12, and the liquid outlet of each DO detector 5 is connected with one waste liquid bottle 12 for collecting the detected waste liquid.
In a specific example, the seed reaction vessel 4 has a spiral shape and a hydrophilic material is attached to the inside thereof in order to promote a microbial reaction.
Preferably, the material of the strain reactor is glass or ceramic.
In a specific embodiment, the liquid inlet of the DO detector is lower than the liquid outlet, and is connected with the liquid outlet of the strain reactor; the solution continuously flows in from the liquid inlet of the DO detector, can be automatically discharged from the liquid outlet after reaching a certain height of water level, and finally flows into a waste liquid bottle.
In a specific embodiment, joints of the pipeline and each part element are not limited, in order to further facilitate maintenance of the equipment, the preferred joints of the pipeline and each part are PEEK heads which can be conveniently disassembled and assembled, and the pipeline is made of plastic materials.
The biochemical reaction testing device for the BOD strain comprises the following testing steps:
(1) starting a main power supply of the instrument, setting a temperature value in the constant-temperature bin, keeping a constant-temperature state, and providing a proper temperature range for detection of a water sample to be detected;
(2) putting a water sample to be detected into a sample liquid bottle, putting a buffer solution into a buffer liquid bottle, opening a peristaltic pump, adjusting the flow rate, and controlling the proportion of the buffer solution and the sample liquid.
(3) According to the index to be measured, putting corresponding strain suspension liquid into each strain reactor, and controlling sample liquid and/or buffer liquid to pass through one strain reactor or a plurality of strain reactors by closing and opening the multi-way valve.
(4) After the strain reaction is finished, carrying out multi-index detection on the reaction liquid through a DO detector, and analyzing.
(5) And finishing the detection analysis, and waiting for next sampling detection.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the utility model, and any modifications, equivalents, improvements and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (9)
1. A biochemical reaction testing device for BOD strains is characterized in that: comprises a sample liquid bottle (1), a first driving element (2), a multi-way valve (3), a plurality of strain reactors (4) and a plurality of DO detectors (5) corresponding to the strain reactors, wherein strains contained in each strain reactor (4) are different; the sample liquid bottle (1), the first driving element (2), the multi-way valve (3), the strain reactor (4) and the DO detector (5) are communicated in sequence through pipelines; the sample liquid is driven by the first driving element (2) to flow into different strain reactors (4) through the multi-way valve (3) respectively for biochemical reaction, and finally, the DO detector (5) is used for carrying out multi-index simultaneous detection on the reaction liquid.
2. The biochemical reaction testing device for BOD species as recited in claim 1, wherein: the strain reactor also comprises a plurality of second driving elements (6), and each strain reactor (4) is communicated with the multi-way valve (3) through one second driving element (6).
3. A biochemical reaction testing device for BOD species as recited in claim 2, wherein: the number of the second driving elements (6), the strain reactor (4) and the DO detector (5) is three; the strain reactor (4) is respectively a nitrifying bacteria strain reactor, a denitrifying bacteria strain reactor and a bacillus strain reactor.
4. A biochemical reaction testing device for BOD species as recited in claim 2, wherein: the device also comprises a constant temperature bin (7) for controlling the temperature of strain reaction, wherein the multi-way valve (3), the second driving element (6), the strain reactor (4) and the DO detector (5) are all positioned inside the constant temperature bin (7).
5. The biochemical reaction testing device for BOD species as recited in claim 1, wherein: the sample liquid bottle device is characterized by further comprising a buffer liquid bottle (8) and a third driving element (9), wherein the buffer liquid bottle (8), the third driving element (9) and the sample liquid bottle (1) are sequentially connected through a pipeline; the buffer solution flows into the sample solution bottle (1) under the drive of the third drive element (9) and is mixed with the sample solution.
6. The biochemical reaction testing device for BOD species as recited in claim 5, wherein: the buffer liquid bottle (8) is connected with the multi-way valve (3) through a pipeline, and a fourth driving element (10) is arranged between the buffer liquid bottle (8) and the multi-way valve (3).
7. A biochemical reaction testing device for BOD bacterial species according to any of claims 1 to 6, wherein: the first driving element (2), the second driving element (6), the third driving element (9) and the fourth driving element (10) are peristaltic pumps, and flow meters (11) are arranged on the peristaltic pumps.
8. The biochemical reaction testing device for BOD species as recited in claim 1, wherein: the device also comprises a plurality of waste liquid bottles (12), and the liquid outlet of each DO detector (5) is connected with one waste liquid bottle (12).
9. The biochemical reaction testing device for BOD species as recited in claim 1, wherein: the strain reactor (4) is spiral.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122561674.7U CN216378222U (en) | 2021-10-25 | 2021-10-25 | Biochemical reaction testing device for BOD strains |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122561674.7U CN216378222U (en) | 2021-10-25 | 2021-10-25 | Biochemical reaction testing device for BOD strains |
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| CN216378222U true CN216378222U (en) | 2022-04-26 |
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| CN202122561674.7U Active CN216378222U (en) | 2021-10-25 | 2021-10-25 | Biochemical reaction testing device for BOD strains |
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2021
- 2021-10-25 CN CN202122561674.7U patent/CN216378222U/en active Active
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