CN216350339U - Microorganism and cell fluorescent staining detection kit - Google Patents
Microorganism and cell fluorescent staining detection kit Download PDFInfo
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- CN216350339U CN216350339U CN202121758725.9U CN202121758725U CN216350339U CN 216350339 U CN216350339 U CN 216350339U CN 202121758725 U CN202121758725 U CN 202121758725U CN 216350339 U CN216350339 U CN 216350339U
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Abstract
The utility model discloses a microbial and cellular fluorescent staining detection kit, which comprises an inner box body, a glass slide, a sample treatment preservation liquid bottle, a fungal fluorescent staining liquid bottle, an anti-fluorescence quenching mirror oil bottle, a bacterial and cellular fluorescent staining liquid bottle, mirror wiping paper and a cover glass, wherein the inner box body is provided with a glass slide groove, a sample treatment preservation liquid groove, a fungal fluorescent staining liquid groove, an anti-fluorescence quenching mirror oil groove, a bacterial and cellular fluorescent staining liquid groove, a mirror wiping paper groove and a cover glass groove; the utility model relates to a microbial and cell fluorescent staining detection kit, which aims at the whole processes of sample treatment and preservation, sample smear, fungus, bacteria, cell fluorescent staining, anti-fluorescence quenching and objective lens maintenance in the microbial and cell fluorescent staining method examination of human body fluid or skin samples, and provides all-round reagent article supply.
Description
Technical Field
The utility model relates to the technical field of biological detection, in particular to a fluorescence staining detection kit for microorganisms and cells.
Background
The detection of fungus, bacteria and cell visible components in human body fluid samples or skin samples mainly depends on the observation of a microscope, and the treatment mode of the samples mainly comprises a common chemical staining microscopy method and a special fluorescent staining microscopy method. The common chemical staining microscopy method is simple and convenient and has high specificity. However, the rate of positive detection is not high, and especially when the number of microorganisms and cells is small, the rate of missed detection often occurs, so that in order to further improve the sensitivity, a special fluorescent staining microscopy method is further developed, and the staining method is divided into a manual fluorescent staining method and an automatic fluorescent staining method. Corresponding fluorescent staining solution is required to be prepared aiming at the automatic fluorescent staining method of the instrument.
Common automatic stainers are only matched with fluorescent staining solution. However, the fluorescent staining detection of human body fluid samples or skin samples is an integral process, and comprises the following steps: sample processing and storing, sample smear fixing, fluorescent staining, anti-fluorescence quenching treatment, objective lens maintenance, observation and interpretation under visual field, and detection report form printing. Second, fungi fluorescent staining observation products are common in the existing market, but it is necessary for correct diagnosis of human diseases to observe bacteria and cells of human body fluids or skin samples in addition to fungi. Thirdly, fluorescence generally has the defect of easy quenching, if quenching is fast, users cannot observe or cannot save observation images so as to cause great inconvenience for clinical examination, and in order to greatly prolong the fluorescence non-attenuation presenting time, the anti-fluorescence quenching mirror oil is essential for fluorescence staining observation. Fourthly, the fluorescence microscope belongs to the high-grade microscope, and the back that finishes using, just involves the problem of how to maintain expensive fluorescence objective lens, and the article that uses when detecting such as current sample smear, sample processing preservation liquid bottle, anti fluorescence quenching mirror oil bottle, glass cover all are single to be placed in the packing carton, need open a plurality of packing cartons during the use and accomodate, be not convenient for use.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a kit for detecting fluorescence staining of microorganisms and cells, which aims to solve the problems in the background technology.
In order to achieve the purpose, the utility model provides the following technical scheme: a microorganism and cell fluorescent staining detection kit comprises an inner box body, a glass slide, a sample treatment preservation liquid bottle, a fungus fluorescent staining liquid bottle, an anti-fluorescence quenching mirror oil bottle, a bacterium and cell fluorescent staining liquid bottle, mirror wiping paper and a cover glass, wherein the inner box body is provided with a glass slide groove, a sample treatment preservation liquid groove, a fungus fluorescent staining liquid groove, an anti-fluorescence quenching mirror oil groove, a bacterium and cell fluorescent staining liquid groove, a mirror wiping paper groove and a cover glass groove, the glass slide is arranged in the glass slide groove, the sample treatment preservation liquid bottle is arranged in the sample treatment preservation liquid groove, the fungus fluorescent staining liquid bottle is arranged in the fungus fluorescent staining liquid groove, the anti-fluorescence quenching mirror oil bottle is arranged in the anti-fluorescence quenching mirror oil groove, the bacterium and cell fluorescent staining liquid bottle is arranged in the bacterium and cell fluorescent staining liquid groove, the lens wiping paper is accommodated in the lens wiping paper groove, and the cover glass is accommodated in the cover glass groove.
When the kit is used specifically, the detection time of the microorganism and cell fluorescent staining detection kit can be shortened, the working efficiency is improved, the reduction of the detection accuracy caused by sample factors is reduced, the normal service life of a fluorescent microscope is prolonged, the manpower is reduced, the overall advantages of the kit can be exerted particularly by matching with an automatic fluorescent staining detection system, and the whole flow of detection of microorganism and cell visible components in a whole human body fluid sample or skin can be optimized by the kit and the automatic fluorescent staining detection system. The method is standardized, efficient, accurate and standardized.
As a preferred technical scheme, the detection kit further comprises an outer packaging box, the inner box body is fixed in the outer packaging box, a protective cover is rotatably mounted on the outer packaging box, and the opening directions of the glass slide groove, the sample treatment preservation solution groove, the fungus fluorescence staining solution groove, the fluorescence quenching mirror oil resisting groove, the fluorescence staining solution groove, the mirror wiping paper groove and the cover glass groove face the protective cover.
When the anti-pollution inner box is used specifically, the inner box body can be protected through the outer packing box, the inner box body is prevented from being polluted, the outer packing box can be opened through the protective cover, and therefore articles in the inner box body can be taken out.
As a preferred technical scheme of the utility model, the sample treatment and preservation solution groove, the fungus fluorescence staining solution groove, the anti-fluorescence quenching mirror oil groove and the bacteria and cell fluorescence staining solution groove are internally provided with fixing buckle seats convenient for fixing reagent bottles.
When the reagent bottle fixing device is used specifically, the reagent bottle can be fixed through the fixing buckle seat, and the reagent bottle is prevented from falling off.
Preferably, the edges of the glass slide groove, the sample treatment preservation solution groove, the fungus fluorescence staining solution groove, the anti-fluorescence quenching mirror oil groove, the bacteria and cell fluorescence staining solution groove, the mirror wiping paper groove and the cover glass groove are all provided with label paper for indicating the category of the reagent in the groove.
When the label paper is used specifically, the articles in the groove can be indicated through the label paper, and the articles in the groove can be conveniently identified.
As a preferable technical scheme of the utility model, the top end of the outer packing box is provided with a handle.
When the external packing box is used specifically, the external packing box can be conveniently lifted up through the lifting handle.
According to a preferable technical scheme of the utility model, a handle groove is formed in the top end of the outer packaging box, the handle is arranged in the handle groove in a sliding mode, and a locking mechanism for locking the protective cover is arranged in the top wall of the outer packaging box.
When the novel multifunctional lifting handle is used specifically, the lifting handle can slide up and down in the lifting handle groove, and the protective cover can be locked through the locking mechanism.
As a preferred technical solution of the present invention, the locking mechanism includes a first rack, a gear and a second rack, the first rack is fixedly mounted at the bottom of the side wall of the handle, the gear is rotatably mounted in the top wall of the outer packing box, the first rack is engaged with the gear, the second rack is slidably mounted in the top wall of the outer packing box, the second rack is engaged with the gear, the first rack and the second rack are parallel to each other, and a fastening column is fixedly mounted at the bottom end of the second rack.
When the lifting handle is pulled upwards in specific use, the first rack moves upwards to drive the gear to rotate, and when the gear rotates, the second rack moves downwards to drive the clamping column to move downwards, the protective cover is locked when the clamping column moves downwards, and when the lifting handle is pressed downwards, the first rack moves downwards to drive the gear to rotate reversely, and when the gear rotates reversely, the second rack moves upwards to drive the clamping column to move upwards, so that the protective cover is unlocked.
As a preferable technical scheme of the utility model, a folded plate is rotatably arranged on one side of the top end of the protective cover, a clamping groove is formed in the top end of the folded plate, and the clamping groove is matched with the clamping column.
During the specific use, when the card post moves down, the card post inserts in the draw-in groove to fix the hem, make the visor can't turn, and then lock the visor.
Compared with the prior art, the utility model has the beneficial effects that: the utility model relates to a microbial and cell fluorescent staining detection kit, which aims at the whole processes of sample treatment and preservation, sample smear, fungus, bacteria, cell fluorescent staining, anti-fluorescence quenching and objective lens maintenance in the microbial and cell fluorescent staining method examination of human body fluid or skin samples, and provides all-round reagent article supply.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic side view of the cross-sectional structure of the present invention;
FIG. 3 is a schematic structural diagram of the inner case of the present invention;
FIG. 4 is a schematic cross-sectional front view of the present invention;
fig. 5 is an enlarged schematic view of a portion a of fig. 4.
In the figure: 1. an inner box body; 101. a glass slide groove; 102. a sample treatment and preservation solution groove; 103. fungus fluorescent staining solution grooves; 104. an anti-fluorescence quenching mirror oil groove; 105. bacteria and cell fluorescent staining solution grooves; 106. a groove for the lens wiping paper; 107. a cover glass groove; 2. packaging the mixture in an external box; 3. a protective cover; 4. a handle; 5. folding the plate; 501. a card slot; 6. a first rack; 7. a gear; 8. a second rack; 9. clamping the column; 11. a glass slide; 12. a sample treatment preservative solution bottle; 13. fungus fluorescent staining solution bottle; 14. an anti-fluorescence quenching mirror oil bottle; 15. bacteria and cell fluorescent staining solution bottles; 16. lens wiping paper; 17. and (5) covering with a glass slide.
Detailed Description
Referring to fig. 1-5, the utility model provides a microorganism and cell fluorescent staining detection kit, comprising an inner box body 1, a glass slide 11, a sample treatment preservation liquid bottle 12, a fungus fluorescent staining liquid bottle 13, an anti-fluorescence quenching mirror oil bottle 14, a bacteria and cell fluorescent staining liquid bottle 15, a mirror wiping paper 16 and a cover glass 17, wherein the inner box body 1 is provided with a glass slide groove 101, a sample treatment preservation liquid groove 102, a fungus fluorescent staining liquid groove 103, an anti-fluorescence quenching mirror oil groove 104, a bacteria and cell fluorescent staining liquid groove 105, a mirror wiping paper groove 106 and a cover glass groove 107, the glass slide 11 is accommodated in the glass slide groove 101, the sample treatment preservation liquid bottle 12 is accommodated in the sample treatment preservation liquid groove 102, the fungus fluorescent staining liquid bottle 13 is accommodated in the fungus fluorescent staining liquid groove 103, the anti-fluorescence quenching mirror oil bottle 14 is accommodated in the anti-fluorescence staining mirror oil groove 104, the bacteria and cell fluorescent staining solution bottle 15 is contained in the bacteria and cell fluorescent staining solution groove 105, the lens wiping paper 16 is contained in the lens wiping paper groove 106, and the cover glass 17 is contained in the cover glass groove 107.
When the kit is used specifically, the detection time of the microorganism and cell fluorescent staining detection kit can be shortened, the working efficiency is improved, the reduction of the detection accuracy caused by sample factors is reduced, the normal service life of a fluorescent microscope is prolonged, the manpower is reduced, the overall advantages of the kit can be exerted particularly by matching with an automatic fluorescent staining detection system, and the whole flow of detection of microorganism and cell visible components in a whole human body fluid sample or skin can be optimized by the kit and the automatic fluorescent staining detection system. The sample treatment and preservation solution can be contained in the sample treatment and preservation solution groove 102, and can effectively preserve the activity of each biological component contained in a human body fluid sample or a skin sample, so that the effects of preventing partial bacteria from autolysis and cell rupture and influencing the accuracy of an inspection result due to the damaged form of the biological component can be prevented, and the interval time of detecting the sample can be further prolonged; the sample processing and preserving fluid contains bioactive substances for processing a human body fluid sample or a skin sample, so that autolysis or cell rupture of partial bacteria is prevented, keratolysis of the skin sample is processed, the sample is processed correctly and timely, the effect of greatly improving the accuracy of an image inspection result is facilitated, the interval time from the sample to detection is prolonged, the inspection operators are greatly facilitated, the fluorescence quenching is easy to perform, the anti-fluorescence quenching mirror oil processing is required, the fluorescence emission time is greatly prolonged, the accurate microbial and cytological observation and preservation of morphological images of the sample by inspectors are facilitated, the objective lens oil is removed to reach a maintenance lens after the expensive fluorescence objective lens is used, the normal service life of a fluorescence microscope is prolonged, and the effect of not influencing the next observation result is ensured.
In this embodiment, 1 bottle of the fungus fluorescence staining solution can be contained in the fungus fluorescence staining solution groove 103, 1 bottle of the cell and microbe fluorescence staining solution can be contained in the bacterium and cell fluorescence staining solution groove 105, the two fluorescence staining solutions are main function executives of the kit, the fungus fluorescence staining solution mainly enables fungi in a human body fluid sample or a skin sample on a slide to emit blue fluorescence, the cell and microbe fluorescence staining solution enables various forms of bacteria and cells in the human body fluid sample or the skin sample on the slide to emit orange or red fluorescence, so that observation under a fluorescence microscope is facilitated, detection accuracy is improved, 1 bottle of the anti-fluorescence quenching mirror oil can be contained in the anti-fluorescence quenching mirror oil groove 104, and the anti-fluorescence quenching mirror oil ensures that fluorescence of the fluorescence-stained sample slide is not easily quenched when the sample slide is placed under the fluorescence microscope, further, the observation is clear, the observation time can be greatly prolonged, 1 box cover glass 17 can be placed in the cover glass groove 107, the cover glass 17 has the effect of protecting a sample and a fluorescence microscope objective, 1 piece of lens wiping paper 16 can be placed in the lens wiping paper groove 106, and the lens wiping paper 16 has the function of removing residual fluorescence-resistant quenching lens oil and a drying lens on the fluorescence objective so as to protect the lens.
Preferably, the detection kit further comprises an outer packing box 2, the inner box body 1 is fixed in the outer packing box 2, the outer packing box 2 is rotatably provided with a protective cover 3, and the opening directions of the glass slide groove 101, the sample treatment preservation solution groove 102, the fungus fluorescence staining solution groove 103, the fluorescence quenching mirror oil resisting groove 104, the fluorescence staining solution groove, the mirror wiping paper groove 106 and the cover glass groove 107 face the protective cover 3.
When the novel box is used specifically, the inner box body 1 can be protected through the outer packing box 2, the inner box body 1 is prevented from being polluted, the outer packing box 2 can be opened through the protective cover 3, and therefore articles in the inner box body 1 can be taken out.
Preferably, the sample treatment preservation solution groove 102, the fungus fluorescence staining solution groove 103, the anti-fluorescence quenching mirror oil groove 104, and the bacteria and cell fluorescence staining solution groove 105 are all provided with fixing buckle seats convenient for fixing reagent bottles.
When the reagent bottle fixing device is used specifically, the reagent bottle can be fixed through the fixing buckle seat, and the reagent bottle is prevented from falling off.
Preferably, the edges of the slide groove 101, the sample treatment preservation solution groove 102, the fungus fluorescence staining solution groove 103, the fluorescence quenching mirror oil resisting groove 104, the bacteria and cell fluorescence staining solution groove 105, the lens wiping paper groove 106 and the cover glass groove 107 are all provided with label paper for indicating the order of the reagents in the grooves.
When the label paper is used specifically, the articles in the groove can be indicated through the label paper, and the articles in the groove can be conveniently identified.
Preferably, a handle 4 is arranged at the top end of the outer packing box 2.
During specific use, the outer packing box 2 can be conveniently lifted up through the lifting handle 4.
Preferably, a handle groove is formed in the top end of the outer packing box 2, the handle 4 is slidably arranged in the handle groove, and a locking mechanism for locking the protective cover 3 is arranged in the top wall of the outer packing box 2.
When the novel multifunctional lifting handle is used specifically, the lifting handle 4 can slide up and down in the lifting handle groove, and the protective cover 3 can be locked through the locking mechanism.
Preferably, the locking mechanism comprises a first rack 6, a gear 7 and a second rack 8, the first rack 6 is fixedly installed at the bottom of the side wall of the handle 4, the gear 7 is rotatably installed in the top wall of the outer packing box 2, the first rack 6 is meshed with the gear 7, the second rack 8 is slidably installed in the top wall of the outer packing box 2, the second rack 8 is meshed with the gear 7, the first rack 6 and the second rack 8 are arranged in parallel, and a clamping column 9 is fixedly installed at the bottom end of the second rack 8.
During the specific use, when upwards pulling handle 4, first rack 6 rebound, thereby drive gear 7 and rotate, when gear 7 rotates, second rack 8 rebound, thereby drive card post 9 rebound, lock visor 3 during card post 9 rebound, when handle 4 is pushed down, first rack 6 rebound, thereby drive gear 7 antiport, when gear 7 rebound, second rack 8 rebound, thereby drive card post 9 rebound, thereby remove visor 3's locking.
Preferably, a folded plate 5 is rotatably arranged on one side of the top end of the protective cover 3, a clamping groove 501 is formed in the top end of the folded plate 5, and the clamping groove 501 is matched with the clamping column 9.
During the specific use, when card post 9 moves down, card post 9 inserts in draw-in groove 501 to fix the hem, make visor 3 can't turn, and then lock visor 3.
The working principle is as follows: the utility model aims at the human body fluid sample or the skin sample to carry out sample treatment, sample smear, microorganism and cell fluorescent staining, anti-fluorescence quenching treatment and under-lens fluorescent observation in the examination of fungus, bacteria and cell fluorescent staining method, provides all-round reagent article supply in the whole process of lens maintenance after use, greatly facilitates operators by using the combination, improves the working efficiency and standardizes the whole detection process, wherein the sample treatment preservation solution contains bioactive substances for treating the human body fluid sample or the skin sample, prevents partial bacteria from autolysis or cell rupture, and carries out skin sample keratolysis treatment, the sample is correctly and timely treated, thereby greatly facilitating the improvement of the accuracy of the image examination result, achieving the purpose of prolonging the interval time from the sample to the detection and greatly facilitating the examination operators. Fluorescence quenching is easy, and anti-fluorescence quenching mirror oil treatment is needed. Greatly improves the fluorescence emission time, is beneficial to the examiner to correctly observe the sample by microorganism and cytology and store morphological images, clears the lens oil of the object lens to achieve the maintenance lens after the expensive fluorescence object lens is used, so as to prolong the normal service life of the fluorescence microscope, ensures that the effect of the next observation result is not influenced, needs to wipe the lens paper 16 for realizing the maintenance lens, and also comprises a cover glass 17 which is beneficial to avoiding the defect that the lens oil is polluted or the sample with poor fixation greatly influences the correct observation of a user because the lens oil is directly contacted with the sample due to the direct contact of the anti-fluorescence quenching lens oil or the sliding of the sample in the lens oil phase, when the handle 4 is pulled upwards, the first rack 6 moves upwards to drive the gear 7 to rotate, when the gear 7 rotates, the second rack 8 moves downwards to drive the clamping column 9 to move downwards, and when the clamping column 9 moves downwards, the protective cover 3 is locked, when handle 4 is pressed down, first rack 6 moves down to drive gear 7 antiport, and when gear 7 antiport, second rack 8 moves up to drive card post 9 rebound, thereby removes the locking of visor 3.
Claims (8)
1. The utility model provides a microorganism and cell fluorescence staining detect reagent box, includes interior box body, slide glass, sample processing preservation liquid bottle, fungi fluorescence staining liquid bottle, anti fluorescence quenching mirror oil bottle, bacterium and cell fluorescence staining liquid bottle, lens wiping paper and cover glass, its characterized in that: the inner box body is provided with a glass slide groove, a sample treatment preservation liquid groove, a fungus fluorescence staining liquid groove, an anti-fluorescence quenching mirror oil groove, a bacterium and cell fluorescence staining liquid groove, a mirror wiping paper groove and a cover glass groove, the glass slide is contained in the glass slide groove, the sample treatment preservation liquid bottle is contained in the sample treatment preservation liquid groove, the fungus fluorescence staining liquid bottle is contained in the fungus fluorescence staining liquid groove, the anti-fluorescence quenching mirror oil bottle is contained in the anti-fluorescence quenching mirror oil groove, the bacterium and cell fluorescence staining liquid bottle is contained in the bacterium and cell fluorescence staining liquid groove, the mirror wiping paper is contained in the mirror wiping paper groove, and the cover glass is contained in the cover glass groove.
2. The kit for detecting fluorescent staining of microorganisms and cells according to claim 1, wherein: the detection kit further comprises an outer packaging box, the inner box body is fixed in the outer packaging box, a protective cover is rotatably mounted on the outer packaging box, and the opening directions of the glass slide groove, the sample treatment preserving fluid groove, the fungus fluorescence staining fluid groove, the anti-fluorescence quenching mirror oil groove, the fluorescence staining fluid groove, the mirror wiping paper groove and the cover glass groove face the protective cover.
3. The kit for detecting fluorescent staining of microorganisms and cells according to claim 1, wherein: and fixing buckle seats are arranged in the sample treatment and preservation solution groove, the fungus fluorescence staining solution groove, the anti-fluorescence quenching mirror oil groove and the bacteria and cell fluorescence staining solution groove.
4. The kit for detecting fluorescent staining of microorganisms and cells according to claim 1, wherein: and the edges of the glass slide groove, the sample treatment preservation solution groove, the fungus fluorescence staining solution groove, the anti-fluorescence quenching mirror oil groove, the bacteria and cell fluorescence staining solution groove, the mirror wiping paper groove and the cover glass groove are all provided with label paper for indicating the reagent category in the groove.
5. The kit for detecting fluorescent staining of microorganisms and cells according to claim 2, wherein: the top end of the outer packing box is provided with a handle.
6. The kit for fluorescence staining detection of microorganisms and cells according to claim 5, wherein: the top end of the outer packing box is provided with a handle groove, the handle is arranged in the handle groove in a sliding mode, and a locking mechanism used for locking the protective cover is arranged in the top wall of the outer packing box.
7. The kit for fluorescence staining detection of microorganisms and cells according to claim 6, wherein: the locking mechanism comprises a first rack, a gear and a second rack, the first rack is fixedly installed at the bottom of the side wall of the handle, the gear is rotatably installed in the top wall of the outer packaging box, the first rack is meshed with the gear, the second rack is slidably installed in the top wall of the outer packaging box, the second rack is meshed with the gear, the first rack and the second rack are arranged in parallel, and a clamping column is fixedly installed at the bottom end of the second rack.
8. The kit for detecting fluorescent staining of microorganisms and cells according to claim 7, wherein: one side of visor top rotates and is provided with the folded plate, the draw-in groove has been seted up on the top of folded plate, the draw-in groove with card post looks adaptation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121758725.9U CN216350339U (en) | 2021-07-30 | 2021-07-30 | Microorganism and cell fluorescent staining detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121758725.9U CN216350339U (en) | 2021-07-30 | 2021-07-30 | Microorganism and cell fluorescent staining detection kit |
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| Publication Number | Publication Date |
|---|---|
| CN216350339U true CN216350339U (en) | 2022-04-19 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202121758725.9U Active CN216350339U (en) | 2021-07-30 | 2021-07-30 | Microorganism and cell fluorescent staining detection kit |
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| CN (1) | CN216350339U (en) |
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2021
- 2021-07-30 CN CN202121758725.9U patent/CN216350339U/en active Active
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