CN2163370Y - Cultivating myocardial cell throbbing transducer - Google Patents

Abstract

The utility model relates to a cultivating myocardial cell throbbing transducer. The light intensity changes generated on a microscope when cultured myocardial cells contract are used as the input signals of the transducer, the input signals of the transducer are changed into electrical signals by a phototriode and the electrical signals are put out. A recording instrument is used to record directly and exactly. The diameter of a transducer shell is as large as the ocular of the microscope. A grid plate in the transducer shell is provided with a light-sensitive hole and the phototriode inputs light signals through the light-sensitive hole. The transducer, which has the advantages of low cost and high sensitivity, can not only stably and accurately record the throbbing frequencies and strength of the cultivating myocardial cells, but also differentially treat and measure the contracting speed of the cultivating myocardial cells.

Description

Cultivating myocardial cell throbbing transducer

The utility model relates to a kind of the record and cultivates the beat sensor of the frequency and the intensity of beating of cardiac muscle cell.Mainly be applicable to biology and medical domain.

At present, it is to adopt video camera and edge device to measure that external record is measured the instrument of cultivating cardiac muscle cell's activity a kind of, it be with the contraction movement that video camera will be cultivated the cardiac muscle cell record the back on televisor playback time with the displacement of edge device mensuration cell.Another kind is to adopt laser equipment to measure, it is to produce diffraction with laser by grating to pass the variation behind the record laser diffraction behind the cardiac muscle cell, though these two kinds of equipment measurement effects that assay method adopted are better, but cost an arm and a leg, and operation is comparatively complicated, present domestic a kind of constant light source of utilizing microscope to produce that has, make the cell that is placed on the microscope when shrinking, produce the strong and weak variation of light, mouth utilizes photoelectric cell that converting optical signals is electric signal by itself making a video recording, the prime amplifier that passes through about 200 times again amplifies the back by the registering instrument record, reflection cellular contraction situation, adopt this quasi-instrument to measure, though instrument is on the low side, sensitivity is lower, and can only write down the frequency of beating, and can not write down the intensity of beating.

It is a kind of cheap that the purpose of this utility model is to provide, and sensitivity is higher, not only can make the register instrument record frequency of beating, the sensor but also the cardiac muscle cell that can write down the intensity of beating is beaten.

The utility model is to realize like this, the variation of the light power that on microscope, produces when utilizing cellular contraction, utilize photoelectric switching circuit to change electric signal into as input signal its variation by sensor and export, its output signal is directly carried out record accurately by register instrument with electric signal.Photoelectric switching circuit adopts highly sensitive phototriode, be connected in series a resistance at its emitter, the resistance two ends are as output terminal, utilization acts on phototriode light and changes, make the collector and emitter change in resistance, cause electric current and change in voltage on the resistance R, it is changed the contraction movement of measuring cell as output signal by voltage drop on the register instrument record resistance.Sensor outer housing is cylindrical, housing diameter should be identical with the microscope ocular diameter, so that after microscopical eyepiece extraction, directly change this sensor, cover top portion is provided with the back up pad greater than housing diameter, in the shell of back up pad, be provided with a screen apart from 12-16mm, the screen center is provided with a photographic hole, phototriode places screen top to make the sensitization window be positioned at the photographic hole place, by the light variable signal that is produced by cultivation cardiac muscle cell contraction movement in this hole input microscope.

The utility model becomes electric signal owing to adopt phototriode will cultivate the light change transitions that cardiac muscle cell's contraction causes, and can directly carry out record by this sensor by registering instrument, so it is not only with low cost, and sensitivity is higher, the frequency of beating of can either stable and accurate record single cultivation cardiac muscle cell or cell mass, can write down the intensity of beating of cultivating cardiac muscle cell or cell mass again, also can carry out differential simultaneously and handle the mensuration contraction speed.

In conjunction with the accompanying drawings the utility model is described in detail below by embodiment:

Fig. 1 is the utility model electrical schematic diagram.

Fig. 2 is the utility model structural representation.

Phototriode VT emitter is serially connected with a resistance R and capacitor C respectively in Fig. 1 electrical schematic diagram, the resistance R resistance must be greater than phototriode VT resistance in the dark, collector a point and resistance R other end b point are as dc voltage input end, and capacitor C other end d point and resistance b point are as signal output part.

Shell in Fig. 2 structural representation (2) is cylindrical, its top is provided with a back up pad (1), in back up pad 14mm place shell, be connected with a screen (3), screen (3) center is provided with a photographic hole (4), circuit board (5) places on the screen (3), and make phototriode sensitization window be positioned at photographic hole (4) and locate, wherein shell (2) diameter should be identical with the microscopical eyepiece diameter dimension of use, and the diameter of back up pad (1) is greater than cylinder blanket (2) diameter.

During use, this sensor is inserted microscope one be sidelong the position of putting eyepiece, connect the sensor dc supply, adjust focal length, the photographic hole position of screen just in time is positioned on the focus of microscope lens barrel, image on the phototriode VT sensitization window so that cultivate the cardiac muscle cell, produce higher sensitivity.When the cultivation cardiac muscle cell shrinks, cause that light changes in the microscope, and act on phototriode VT, make change in resistance between the collector and emitter, thereby the pressure drop on the resistance R is changed, by voltage signal on the physiology polygraph record resistance R, can write down the activity of beating of cultivating the cardiac muscle cell accurately.

Claims (2)

1, a kind of cultivation cardiac muscle cell sensor of beating, adopt photoelectric switching circuit, it is characterized in that adopting in the photoelectric switching circuit highly sensitive phototriode (VT), the phototriode emitter is connected in series a resistance (R), export phototriode (VT) electrical signal converted as output terminal at resistance (R) two ends, sensor outer housing (1) is cylindrical, shell (2) top is provided with the back up pad (1) greater than shell (2) diameter, be provided with screen (3) in the shell (2), the screen center is provided with a photographic hole (4), and phototriode (VT) places screen top to make the sensitization window be positioned at photographic hole (4) and locates.

2, the cultivation cardiac muscle cell according to claim 1 sensor of beating it is characterized in that the screen (3) and the distance of back up pad (1) are 12-16mm, and the resistance R resistance should be greater than phototriode (VT) resistance in the dark.