CN216314594U - Chuzhou chrysanthemum seed tissue culture sterilization apparatus - Google Patents
Chuzhou chrysanthemum seed tissue culture sterilization apparatus Download PDFInfo
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- CN216314594U CN216314594U CN202122513443.9U CN202122513443U CN216314594U CN 216314594 U CN216314594 U CN 216314594U CN 202122513443 U CN202122513443 U CN 202122513443U CN 216314594 U CN216314594 U CN 216314594U
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Abstract
The utility model discloses a Chuzhou chrysanthemum seed tissue culture sterilization device which comprises a support, at least three liquid storage tanks, a sterilization barrel, a grid tray and filter paper; the liquid storage tank is fixed on the bracket, and the bottom of the liquid storage tank is connected with a flexible pipe of the liquid storage tank through a metering valve; the sterilization barrel is fixed on the bracket and is positioned below the liquid storage tank, so that the bottom end of a hose of the liquid storage tank can be placed in the sterilization barrel, the upper part of the sterilization barrel is a cylinder, the lower part of the sterilization barrel is a semi-cone, and the bottom of the sterilization barrel is connected with a drain pipe through a drain valve; the size of the grid tray is matched with the bottom of the sterilization barrel and is used for being placed at the bottom of the sterilization barrel, at least two slings are arranged at the outer edge of the grid tray, and the other ends of the slings are connected with hooks used for being buckled at the opening of the sterilization barrel; the filter paper is matched with the grid tray in size. The Chuzhou chrysanthemum seed tissue culture sterilization device can perform a sterilization procedure in a flow manner, and can easily collect sterilized fine seeds on filter paper.
Description
Technical Field
The utility model relates to a Chuzhou chrysanthemum seed tissue culture sterilization device.
Background
Before the seed tissue culture, the seeds need to be disinfected and sterilized, usually more than two kinds of sterilizing solutions need to be used for treating the seeds, and the seeds are washed by sterile water, so that the operation process is complicated. For the seeds with larger volume, such as peach and apricot, the seeds are not easy to run off when the treatment solution is poured in the sterilization process, and the seeds are clamped out by using tweezers after sterilization, but for the plants, such as chrysanthemum, Chinese cabbage and the like, the seed volume is small, the seeds are easy to flow out along with the liquid when the treatment solution is poured in the sterilization process, and the seeds are difficult to collect after sterilization. To climbing etc. (application number 201821836982.8) provide a tiny seed disinfection container device, though solved the antiseptic solution and emptyd the easy problem of losing of in-process seed, but its operation need use centrifuge, and it is inconvenient to place centrifuge operation on the superclean bench, if take out superclean bench to the centrifuging tube, then cause the pollution easily. In order to solve the problems, a more convenient and efficient tissue culture disinfection and sterilization device for tiny seeds is designed.
SUMMERY OF THE UTILITY MODEL
The utility model aims to overcome the defects and provide the Chuzhou chrysanthemum seed tissue culture sterilization device which can improve the sterilization efficiency of small seeds.
The utility model is realized by the following technical scheme:
a Chuzhou chrysanthemum seed tissue culture sterilization device comprises a support, a plurality of liquid storage tanks, a sterilization barrel, a grid tray and filter paper;
the liquid storage tank is fixed on the bracket, and the bottom of the liquid storage tank is connected with a flexible pipe of the liquid storage tank through a metering valve;
the sterilization barrel is fixed on the bracket and is positioned below the liquid storage tank, so that the bottom end of a hose of the liquid storage tank can be placed in the sterilization barrel, the upper part of the sterilization barrel is a cylinder, the lower part of the sterilization barrel is a semi-cone, and the bottom of the sterilization barrel is connected with a drain pipe through a drain valve;
the size of the grid tray is matched with the bottom of the sterilizing barrel and is used for being placed at the bottom of the sterilizing barrel, at least two slings are arranged at the outer edge of the grid tray, and the other ends of the slings are connected with hooks used for being buckled on the wall of the sterilizing barrel;
the filter paper is matched with the grid tray in size.
Preferably, the sterilizing device is further provided with a stirring device, the stirring device is arranged on the barrel wall at the upper part of the sterilizing barrel, and the position of the stirring device is preferably selected so that the stirring device does not touch the sling during operation.
Preferably, the slings are evenly distributed at the outer edge of the grid tray.
Preferably, the waste liquid tank is further arranged, the drain pipe is communicated with the waste liquid tank, and a liquid discharge outlet is formed in the bottom of the waste liquid tank.
Preferably, the bottom opening of the flexible pipe of the liquid storage tank and the flexible pipe of the liquid storage tank form a certain included angle.
Preferably, the fine seeds are chrysanthemum seeds.
Preferably, the fine seeds are Chuzhou chrysanthemum seeds.
The Chuzhou chrysanthemum seed tissue culture sterilization device is made of a material which can withstand the high temperature of at least 200 ℃, such as metal or high-temperature-resistant resin, so that sterilization treatment can be conveniently carried out.
The Chuzhou chrysanthemum seed tissue culture sterilization device is provided with a funnel-shaped sterilization barrel, a grid tray is placed at the bottom of the sterilization barrel, filter paper is placed on the grid tray, seeds are placed in the sterilization barrel, appropriate solution is injected into the sterilization barrel from a liquid storage tank according to a tissue culture sterilization program, the sterilization solution or sterilization water is stirred to complete the sterilization or cleaning program, then a drain valve at the bottom of the sterilization barrel is opened, liquid in the sterilization barrel is discharged, and liquid injection is continued to perform the next step of program until all tissue culture sterilization programs are completed. Then the mesh tray and the filter paper on the mesh tray are taken out by lifting the sling, and the sterilized fine seeds on the filter paper can be collected.
Drawings
Fig. 1 is a schematic structural view of the chuzhou chrysanthemum seed tissue culture sterilization apparatus without a grid tray in embodiment 1.
Fig. 2 is a schematic structural diagram of the grid tray.
Fig. 3 is a schematic structural view of the Chuzhou chrysanthemum seed tissue culture sterilization device placed in the grid tray in embodiment 1.
Fig. 4 is a schematic structural view of the Chuzhou chrysanthemum seed tissue culture sterilization device in embodiment 2 without placing a grid tray.
Detailed Description
Example 1
Fig. 1-3 show a specific embodiment of the chuzhou chrysanthemum seed tissue culture sterilization device, and the structure of the chuzhou chrysanthemum seed tissue culture sterilization device is described in detail below with reference to the accompanying drawings.
A Chuzhou chrysanthemum seed tissue culture sterilization device comprises a support 1, three liquid storage tanks 2, a sterilization barrel 3, a grid tray 4 and filter paper (not shown in the figure);
the liquid storage tank 2 is fixed on the bracket 1, and the bottom of the liquid storage tank is connected with a flexible pipe 6 of the liquid storage tank through a metering valve 5;
the sterilization barrel is fixed on the bracket and is positioned below the liquid storage tank, so that the bottom end of a hose of the liquid storage tank can be placed in the sterilization barrel, the upper part of the sterilization barrel is a cylinder, the lower part of the sterilization barrel is a semi-cone, and the bottom of the sterilization barrel is connected with a drain pipe 8 through a drain valve 7;
the size of the grid tray is matched with the bottom of the sterilizing barrel and is used for being placed at the bottom of the sterilizing barrel, at least two slings 9 are arranged at the outer edge of the grid tray and are uniformly distributed at the outer edge of the grid tray, and the other ends of the slings are connected with hooks 10 used for being buckled on the wall of the sterilizing barrel;
the filter paper is matched with the grid tray in size.
The sterilizing bucket is characterized by further comprising a stirring device 11, wherein the stirring device is arranged on the bucket wall at the upper part of the sterilizing bucket, and the position of the stirring device is selected so that the stirring device does not touch the sling when in operation. The stirring device is not an essential component, and can be directly stirred by a glass rod.
And a waste liquid pool 12 is further arranged, the drain pipe is communicated with the waste liquid pool, and a liquid discharge outlet is formed in the bottom of the waste liquid pool. The waste liquid pool can be arranged independently or fixed on the bracket. The waste liquid tank is not an essential component, and the liquid in the drain pipe 8 can be directly drained into the water tank.
Taking Chuzhou chrysanthemum seeds as an example, the operation process of Chuzhou chrysanthemum tissue culture is as follows:
1. disinfecting the seeds for 8-12min by 30mL of 2% sodium hypochlorite;
2. washing with 40mL of sterile water for 2-3 times;
3. 30mL of 75% alcohol for 20-30 s;
4. 40mL of sterile water was washed 2-3 times.
5. Seeds were removed on sterile filter paper.
When the chrysanthemum seed tissue culture sterilization device is used, the chrysanthemum seed tissue culture sterilization device comprises a grid tray and filter paper, and sterilization treatment is firstly carried out on the chrysanthemum seed tissue culture sterilization device, and all operations are carried out on a super clean bench;
placing the grid tray on which the filter paper is laid at the bottom of a sterilization barrel, hanging a hook on the wall of the sterilization barrel, and placing the Chuzhou chrysanthemum seeds to be subjected to tissue culture into the sterilization barrel;
respectively adding 2% of sodium hypochlorite solution, sterile water and 75% of alcohol into the three liquid storage tanks, opening a metering valve of the liquid storage tank filled with the sodium hypochlorite solution, extending a corresponding liquid storage tank hose into the sterilization barrel, closing the metering valve after 30mL of the sodium hypochlorite solution flows into the sterilization barrel, stirring the sodium hypochlorite solution by using a stirring device or a glass rod, opening a drain valve after 8-12min, discharging the sodium hypochlorite solution from a drain pipe, and discharging waste liquid into a waste liquid tank or a water tank;
opening a metering valve of a liquid storage tank filled with sterile water, extending a corresponding hose of the liquid storage tank into a sterilization barrel, closing the metering valve after 40mL of sterile water flows into the sterilization barrel, stirring the sterile water by using a stirring device or a glass rod, and then opening a drain valve, so that the sterile water is discharged from a drain pipe; repeating the step for 2-3 times;
opening a metering valve of a liquid storage tank filled with 75% alcohol, extending a corresponding hose 6 of the liquid storage tank into a sterilization barrel, closing the metering valve after 30mL of 75% alcohol flows into the sterilization barrel, stirring the alcohol solution for 20-30s by using a stirring device or a glass rod, and then opening a drain valve, and discharging the alcohol solution from a drain pipe;
opening a metering valve of a liquid storage tank filled with sterile water, extending a corresponding hose 6 of the liquid storage tank into a sterilization barrel, closing the metering valve after 40mL of sterile water flows into the sterilization barrel, stirring the sterile water by using a stirring device or a glass rod, and then opening a drain valve, and discharging the sterile water from a drain pipe; repeating the step for 2-3 times, lifting the sling, taking out the grid tray, and taking down the filter paper, wherein the filter paper is the sterilized Chuzhou chrysanthemum seeds.
Example 2
As shown in FIG. 4, the structure of this embodiment is substantially the same as that of embodiment 1, except that the bottom of the hose 6 of the liquid storage tank is a bevel, and the bottom opening and the hose 6 of the liquid storage tank are designed to form a certain included angle. The flexible pipe of the liquid storage tank is controlled to inject liquid around the side of the side wall of the sterilization barrel, so that seeds on the side wall of the sterilization barrel can be washed off, and the seeds are prevented from being stuck to the wall.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (7)
1. A Chuzhou chrysanthemum seed tissue culture sterilization device comprises a support, a plurality of liquid storage tanks, a sterilization barrel, a grid tray and filter paper;
the liquid storage tank is fixed on the bracket, and the bottom of the liquid storage tank is connected with a flexible pipe of the liquid storage tank through a metering valve;
the sterilization barrel is fixed on the bracket and is positioned below the liquid storage tank, so that the bottom end of a hose of the liquid storage tank can be placed in the sterilization barrel, the upper part of the sterilization barrel is a cylinder, the lower part of the sterilization barrel is a semi-cone, and the bottom of the sterilization barrel is connected with a drain pipe through a drain valve;
the size of the grid tray is matched with the bottom of the sterilizing barrel and is used for being placed at the bottom of the sterilizing barrel, at least two slings are arranged at the outer edge of the grid tray, and the other ends of the slings are connected with hooks used for being buckled on the wall of the sterilizing barrel;
the filter paper is matched with the grid tray in size.
2. The Chuzhou chrysanthemum seed tissue culture sterilization device of claim 1, characterized in that: and the stirring device is arranged on the barrel wall at the upper part of the sterilization barrel.
3. The Chuzhou chrysanthemum seed tissue culture sterilization device of claim 1 or 2, characterized in that: the suspension ropes are uniformly distributed on the outer edge of the grid tray.
4. The Chuzhou chrysanthemum seed tissue culture sterilization device of claim 1 or 2, characterized in that: still be equipped with the waste liquid pond, the drain pipe UNICOM arrives the waste liquid pond, waste liquid bottom of the pool portion is equipped with the flowing back export.
5. The Chuzhou chrysanthemum seed tissue culture sterilization device of claim 1 or 2, characterized in that: the bottom opening of the liquid storage tank hose and the liquid storage tank hose form a certain included angle.
6. The Chuzhou chrysanthemum seed tissue culture sterilization device of claim 1 or 2, characterized in that: the fine seeds are chrysanthemum seeds.
7. The Chuzhou chrysanthemum seed tissue culture sterilization device of claim 5, characterized in that: the small seeds are Chuzhou chrysanthemum seeds.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122513443.9U CN216314594U (en) | 2021-10-19 | 2021-10-19 | Chuzhou chrysanthemum seed tissue culture sterilization apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122513443.9U CN216314594U (en) | 2021-10-19 | 2021-10-19 | Chuzhou chrysanthemum seed tissue culture sterilization apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216314594U true CN216314594U (en) | 2022-04-19 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122513443.9U Active CN216314594U (en) | 2021-10-19 | 2021-10-19 | Chuzhou chrysanthemum seed tissue culture sterilization apparatus |
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| CN (1) | CN216314594U (en) |
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2021
- 2021-10-19 CN CN202122513443.9U patent/CN216314594U/en active Active
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