CN216018356U

CN216018356U - Explant sterilizing device in plant tissue culture process

Info

Publication number: CN216018356U
Application number: CN202122180821.6U
Authority: CN
Inventors: 马燕欣; 郎淑平; 沈盟; 王瑞森; 袁晔; 姚祥坦; 权新华
Original assignee: JIAXING ACADEMY OF AGRICULTURAL SCIENCES
Current assignee: JIAXING ACADEMY OF AGRICULTURAL SCIENCES
Priority date: 2021-09-09
Filing date: 2021-09-09
Publication date: 2022-03-15
Anticipated expiration: 2031-09-09

Abstract

The utility model discloses an explant disinfection device in a plant tissue culture process, which comprises a beaker for disinfection and is characterized by also comprising a circular ring (3) and a blocking device, wherein the diameter of the circular ring (3) is larger than that of the outer wall of the beaker and is sleeved on the outer wall of the beaker; a plurality of hooks (4) are uniformly arranged on the outer wall of the circular ring (3) in the circumferential direction, and the opening direction of the hooks (4) is downward; the blocking net device is movably connected with the circular ring (3) through a hook (4) and is used as a top cover to cover the beaker during connection. The device is used in cooperation with the beaker when the explant is sterilized, the mesh cover is formed at the mouth of the beaker, the disinfectant and sterile water can be directly poured into the beaker through the mesh cover, the explant cannot float after the disinfectant is poured due to the blocking of the cover, the explant does not need to be continuously pressed by tweezers, the explant cannot fall off in the waste liquid pouring process due to the blocking of the cover, the waste liquid can be completely poured in, and the rinsing is more sufficient.

Description

Explant sterilizing device in plant tissue culture process

Technical Field

The utility model relates to the field of tissue culture, in particular to the field of plant tissue culture vessels, and specifically relates to an explant disinfection device in a plant tissue culture process.

Background

A beaker is selected as a disinfection container in the plant culture process, and when explants (such as rice ears, wheat ears and the like) are disinfected, the explants need to be continuously pressed by tweezers, so that the explants are fully contacted with a disinfectant, otherwise, the explants cannot be thoroughly disinfected. The explant is easy to drop when pouring the liquid in the beaker, or the liquid is difficult to pour out cleanly, and disinfectant residue is easy to cause.

The pollution rate reduction in the plant tissue culture process is the most key factor for improving the tissue culture efficiency, and the explant disinfection is the most important step for reducing the pollution rate. At present, a beaker is used as a container for sterilizing explants in the tissue culture process, long explants (such as rice ears) are vertically placed in the beaker, and short or blocky explants are directly placed in the beaker. After the disinfectant is added, the explants float up, influenced by the disinfectant and the specific gravity of the explants. Due to the fact that the number of disinfectants required to be poured into the explants such as rice ears is large, the ears can fall out of the beaker along with the increase of the volume of the disinfectants, the fallen explants cannot be used, and otherwise, subsequent experiment pollution is easily caused. In order to ensure that the explant is fully contacted with the disinfectant, the explant needs to be continuously pressed by using tweezers, otherwise incomplete disinfection of the explant is easily caused, and subsequent experiments are influenced. After the disinfectant treatment is finished, the disinfectant needs to be poured out, and then the explants are rinsed for multiple times by using sterile water, so that the explants are prevented from being poisoned by residual disinfectant. In actual operation, when the disinfection solution and the sterile water for rinsing are poured, due to the worry that the explants fall, a part of liquid is usually left at the bottom of the beaker, and the washing is not thorough, so that the disinfection solution remains in the explants, and toxic action is generated on the explants.

SUMMERY OF THE UTILITY MODEL

The utility model provides an explant disinfection device in a plant tissue culture process, which aims at solving the problems in the background art and is matched with a beaker in the explant disinfection process.

The technical scheme is as follows:

an explant disinfection device in a plant tissue culture process comprises a circular ring and a blocking device, wherein the diameter of the circular ring is larger than that of the outer wall of a beaker, and the circular ring is sleeved on the outer wall of the beaker; a plurality of hooks are uniformly arranged on the outer wall of the circular ring in the circumferential direction, and the opening direction of the hooks is downward; the blocking net device is movably connected with the circular ring through a hook and used as a top cover to cover the beaker during connection.

As an implementation of the barrier device: the net blocking device is a net cover which is in a round cake shape, and the diameter of the net cover is consistent with or slightly larger than that of the round ring; the edge of net lid evenly fixes a plurality of springs, and the quantity of spring is the integral multiple of the quantity of couple, and the spring matches with the couple and realizes blocking the active link of net device and ring.

The mesh density of the mesh cover is 7 meshes.

The diameter of the spring is 4 mm.

Preferably, the diameter of the mesh cover is greater than the diameter of the circular ring by 5 mm.

As another implementation of the barrier device: the hooks are arranged at the top of the circular ring, the number of the hooks is even, and the two opposite hooks penetrating through the circle center are hooked by rubber bands to form a blocking net device.

In the whole scheme, the hook is in a water drop shape, and the size is 3mm by 3 mm.

In the whole scheme, the width of the circular ring is 13 mm.

In the whole scheme, the circular ring and the hook are made of stainless steel.

In the overall scheme, the device is autoclaved and can be contacted with various types of disinfectants.

The utility model has the advantages of

The device is used in cooperation with the beaker when the explant is sterilized, the mesh cover is formed at the mouth of the beaker, the disinfectant and sterile water can be directly poured into the beaker through the mesh cover, the explant cannot float after the disinfectant is poured due to the blocking of the cover, the explant does not need to be continuously pressed by tweezers, the explant cannot fall off in the waste liquid pouring process due to the blocking of the cover, the waste liquid can be completely poured in, and the rinsing is more sufficient.

Drawings

Fig. 1 is a schematic view of one implementation of a barrier net apparatus.

Fig. 2 is a schematic view of another embodiment of a barrier net apparatus.

Detailed Description

The utility model is further illustrated by the following examples, without limiting the scope of the utility model:

example 1:

with reference to fig. 1, an explant sterilization device in a plant tissue culture process comprises a beaker 1 for sterilization, a circular ring 3 and a barrier device, wherein the diameter of the circular ring 3 is larger than that of the outer wall of the beaker and is sleeved on the outer wall of the beaker; a plurality of hooks 4 (the number of the hooks is preferably 3) are annularly and uniformly arranged on the outer wall of the circular ring 3, and the opening direction of the hooks 4 is downward; the blocking net device is movably connected with the circular ring 3 through a hook 4 and is used as a top cover to cover the beaker during connection.

The net blocking device is a net cover 1, the net cover 1 is in a round cake shape, and the diameter of the net cover 1 is larger than that of the circular ring 3 by 5 mm; the edge of the net cover 1 is uniformly fixed with a plurality of springs 2, the number of the springs 2 is an integral multiple of the number of the hooks 4 (in the embodiment, the number is 1 time), and the springs 2 are matched with the hooks 4 to realize the movable connection of the blocking net device and the circular ring 4.

The mesh density of the mesh cover 1 is 7 meshes.

The diameter of the spring 4 is 4 mm.

When the explants are bunched rice ears or wheat ears, the net cover 1 in fig. 1 can be taken down, the rubber bands 6 are hung on the hooks 4 with symmetrical corners, and the function of fixing the explants can also be achieved, and the specific scheme is described in example 2.

Example 2:

with reference to fig. 2, the explant sterilization device in the plant tissue culture process comprises a beaker 1 for sterilization, a circular ring 3 and a barrier device, wherein the diameter of the circular ring 3 is larger than that of the outer wall of the beaker and is sleeved on the outer wall of the beaker; a plurality of hooks 4 (in the embodiment, the number of the hooks is 6) are annularly and uniformly arranged on the outer wall of the ring 3, and the opening direction of the hooks 4 is downward; the blocking net device is movably connected with the circular ring 3 through a hook 4 and is used as a top cover to cover the beaker during connection.

The hooks 4 are arranged at the top of the circular ring 3, the number of the hooks 4 is even, and the two opposite hooks 4 penetrating through the circle center are hooked by the rubber band 6 to form a blocking net device.

Preferably, in example 1 and example 2: the hook 4 is in a drop shape, and the size is 3mm by 3 mm. The width of the ring 3 is 13 mm. The material of the circular ring 3 and the hook 4 is stainless steel. The entire device is autoclaved and can be contacted with various types of disinfectants.

The device is matched with the beaker 1 for use and is convenient to disassemble, the diameter of the circular ring 3 can be selected according to the size of the beaker 1, and the blocking net device is net-shaped and can directly pour in or out liquid. Specifically, the method comprises the following steps:

the diameter of the circular ring 3 comprises four specifications of 140mm, 115mm, 90mm and 75mm, and is respectively suitable for 2000mL, 1000mL, 500mL and 250mL beakers 1.

The spring 2 is 60mm, 45mm, 40mm and 35mm in length, and is suitable for 2000mL, 1000mL, 500mL and 250mL beakers 1.

The specific embodiments described herein are merely illustrative of the spirit of the utility model. Various modifications or additions may be made to the described embodiments or alternatives may be employed by those skilled in the art without departing from the spirit or ambit of the utility model as defined in the appended claims.

Claims

1. The explant disinfection device in the plant tissue culture process comprises a beaker for disinfection, and is characterized by further comprising a circular ring (3) and a blocking device, wherein the diameter of the circular ring (3) is larger than that of the outer wall of the beaker and is sleeved on the outer wall of the beaker; a plurality of hooks (4) are uniformly arranged on the outer wall of the circular ring (3) in the circumferential direction, and the opening direction of the hooks (4) is downward; the blocking net device is movably connected with the circular ring (3) through a hook (4) and is used as a top cover to cover the beaker during connection.

2. The device according to claim 1, characterized in that the net device is a net cover (1), the net cover (1) is in a shape of a circular cake, the diameter of the net cover (1) is the same as or slightly larger than that of the circular ring (3); a plurality of springs (2) are uniformly fixed at the edge of the net cover (1), the number of the springs (2) is integral multiple of the number of the hooks (4), and the springs (2) are matched with the hooks (4) to realize movable connection of the blocking net device and the circular ring (3).

3. The device according to claim 2, characterized in that the mesh density of the mesh cover (1) is 7 mesh.

4. Device according to claim 2, characterized in that the diameter of the spring (2) is 4 mm.

5. Device according to claim 2, characterized in that the diameter of the mesh cover (1) is greater than the diameter of the ring (3) by 5 mm.

6. The device according to claim 1, characterized in that the hooks (4) are arranged on the top of the ring (3), the number of hooks (4) is even, and the two opposite hooks (4) passing through the center of the circle are hooked by a rubber band (6) to form a barrier device.

7. Device according to claim 1, characterized in that the hook (4) is drop-shaped and has a size of 3mm by 3 mm.

8. Device according to claim 1, characterized in that the width of the ring (3) is 13 mm.

9. Device according to claim 1, characterized in that the material of the ring (3) and the hook (4) is stainless steel.