CN216303815U - Liquid feeding accessory and culture dish external member - Google Patents

Abstract

The application discloses a liquid adding accessory, which comprises a base body and a plurality of protruding parts, wherein the base body is in a sheet shape and is provided with one or more openings; the protruding portion is followed the base member extends and the equipartition in the base member periphery, the protruding portion has smooth arc contour line, protruding portion and base member integrated into one piece. The application also discloses a culture dish external member, include according to the liquid feeding accessory and culture dish of this application, the culture dish is provided with the opening, the size of open-ended size more than or equal to base member for the liquid feeding accessory can closely cooperate with the opening, and base member periphery, adjacent protruding portion and culture dish inner wall restrict out the groove. According to the culture dish kit, the culture solution is poured from the limited groove and flows down from the periphery along the wall of the culture dish, so that the aggregated cells at the bottom of the culture dish are basically not impacted; the liquid adding fitting is provided with an exhaust structure, so that the inside of the container can be smoothly exhausted in the liquid adding process.

Description

Liquid feeding accessory and culture dish external member

Technical Field

The application relates to the field of medical equipment, in particular to a liquid feeding accessory and a culture dish suite.

Background

In the process of culturing the chondrocyte membrane, a specially-made high culture dish is required, and with the increase of maturity of chondrocytes in the culture dish, the cells are required to be deposited at the bottom of the culture dish and gradually gathered together to form the membrane with uniform thickness. The culture solution needs to be added periodically, but because the height of the special culture dish is larger, when the diaphragm is not formed stably, the liquid column can not be controlled to remain attached to the wall in the process of pouring the culture solution, the impact force of pouring the liquid is easy to disperse the aggregated cells, the thickness of the diaphragm is not uniform enough, and the loss of the cells is caused in the process of changing the liquid.

Disclosure of Invention

The purpose of this application one side lies in providing a liquid feeding accessory for the culture solution is pouring the in-process and gently leaves from the culture dish adherence all around, will reduce to minimumly to the impact of the aggregated chondrocyte in culture dish bottom.

The application discloses liquid feeding accessory includes: a base body and a plurality of projections, wherein

The substrate is sheet-shaped and is provided with one or more openings;

the protruding parts extend from the base body and are uniformly distributed on the periphery of the base body, and the protruding parts are provided with smooth arc-shaped contour lines;

the protrusion and the base are integrally formed.

In a preferred embodiment, the filling fitting is made of medical silicone rubber.

In a preferred embodiment, the base body has an opening, to which a hard tube with a comparable diameter is detachably fastened.

In a preferred embodiment, the substrate has 2 openings, the 2 openings being closely adjacent to each other and defining an elongated strip-like communication portion therebetween.

In a preferred embodiment, the height of the hard tube is h, wherein the value range of h is 5-10 cm.

In a preferred embodiment, the aperture of the opening is d, wherein the value range of d is 0.5-1 cm.

In a preferred embodiment, the base body is provided in a circular or rectangular shape.

In a preferred example, the number of the protruding parts is n, wherein the value range of n is 2-6.

Another aspect of the present application is to provide a culture dish kit comprising: the filling accessory according to any of the preceding claims, and

the culture dish, the culture dish is provided with the opening, the size of opening more than or equal to the size of base member makes the liquid feeding accessory can with the opening closely cooperates, base member periphery, adjacent protruding portion and culture dish inner wall restrict out the groove.

Preferably, the upper mouth edge of the culture dish is longer than the bottom, and the section of the culture dish body is continuously reduced from top to bottom.

In a preferred example, the height of the culture dish is H, wherein the value range of H is 4-10 cm.

The liquid feeding accessory and the culture dish kit according to the application have at least the following beneficial effects:

1. the poured culture solution is poured from the limited groove and flows down from the periphery along the wall of the culture dish in a gentle way, so that the impact on the aggregated cells at the bottom of the culture dish is basically avoided;

2. when in use, the liquid adding accessory can be convex at the arc top at the middle part, so that liquid is effectively guided to flow downwards and prevented from accumulating;

3. the hard pipe provides a portable function and is responsible for exhausting air, so that the conflict between the exhaust air and liquid inlet is avoided;

4. the hard tube of the liquid adding accessory is detachably connected with the flaky substrate, so that the liquid adding accessory is convenient to disinfect and replace, can be used for one time, can be repeatedly disinfected and is convenient and sanitary;

5. the culture dish in the kit has a shape with a small bottom opening, and the inclined wall of the culture dish is favorable for liquid to flow down along the wall;

6. the material is convenient to obtain, the processing is simple, and the die sinking and separate processing are not needed.

Drawings

FIG. 1 is a schematic view of a filling accessory according to one embodiment of the present application

FIG. 2 is a connection portion according to one embodiment of the present application

FIG. 3 is a schematic view of a refill fitting according to another embodiment of the present application

FIG. 4 is a schematic view of a culture dish kit according to the present application

Description of reference numerals:

01-culture dish

10-liquid feeding accessory

110-base

111-opening of pores

120-projection

121-first projection

122-second projection

123-third projection

124-fourth projection

130-connecting part

131-hard tube

20-liquid feeding accessory

210-base

211-first opening hole

212-second opening hole

Detailed Description

In the following description, numerous technical details are set forth in order to provide a better understanding of the present application. However, it will be understood by those skilled in the art that the technical solutions claimed in the present application may be implemented without these technical details and with various changes and modifications based on the following embodiments.

The filling attachment 10 shown in fig. 1 comprises a rectangular sheet-like base 110 and four protrusions 120, the protrusions 120 comprising a first protrusion 121, a second protrusion 122, a third protrusion 123, and a fourth protrusion 124, each protrusion 120 being located on the same plane as the base 110 and extending from four vertices of the base 110, being arranged on the periphery of the base, and having a smooth curved contour.

The four protrusions 120 and the base 110 are an integrally formed structure made of medical silicone rubber. The length or the width of the liquid adding fitting can be optionally between 5 and 10 cm.

The center of the substrate 110 has an opening 111, and the opening 111 has a diameter d, optionally 0.5. ltoreq. d.ltoreq.1 cm.

The hard tube 131 is detachably and tightly connected to the opening 111, and the aperture of the hard tube 131 is equivalent to that of the opening 111. The hard tube is made of a high polymer material; the height is h, the value range of h is more than or equal to 5 and less than or equal to 10cm, preferably, h is more than or equal to 6 and less than or equal to 8 cm.

The rigid tube 131 is directly inserted into the base body. Optionally, a connecting portion 130 is provided between the hard tube 131 and the opening 111, as shown in fig. 2, the connecting portion 130 has a first port and a second port, the first port is expanded with the opening, and the hard tube 131 is expanded with the second port, so as to achieve a tight connection.

In another embodiment, as shown in FIG. 3, the substrate 210 has 2 openings: a first opening 211 and a second opening 212. The first aperture 211 and the second aperture 212 are closely adjacent, defining an elongated strip-like communication portion therebetween. The central region of the substrate 210 is thin and the periphery is thick.

In another embodiment, the substrate 210 is provided in a circular shape. The number of the protruding parts is n, wherein the value range of n is 2-6.

The invention also discloses a culture dish kit which comprises the liquid adding accessory and a culture dish 01, and is shown in figure 4. The culture dish 01 is an open container, the height range is 5-10 cm, and the top is provided with an opening. Optionally, the bottom and the opening of the culture dish are square and have a shape with a small bottom, and the size of the opening is larger than or equal to that of the substrate. Alternatively, the culture dish is a cylindrical container with a uniform bottom opening, such as a cylindrical open container, or a cubic cylindrical container.

To make the objects, technical solutions and advantages of the present application more clear, embodiments of the present application will be described in further detail below with reference to the accompanying drawings.

Example 1

As shown in fig. 4, the chondrocyte membrane sheets were cultured using the culture dish kit according to the present invention.

The culture dish 01 is an open container with a small opening at the bottom, the height is 8cm, the bottom is a rectangle of 4cm multiplied by 4cm, and the top is a rectangle opening of 6cm multiplied by 6 cm. The size of the base body of the liquid adding fitting 10 is about less than 6cm multiplied by 6cm, the length of the hard pipe is 8cm, and the base body and the hard pipe are tightly connected through a connecting part.

Chondrocytes were deposited on the bottom of the culture dish 01 for culture. The hard pipe 131 of the handheld liquid feeding accessory 10 of operator, put into culture dish 01 opening, because base member 110 size only slightly is less than the opening, push down earlier in the middle part of the base member after the in-process of pushing down, because culture dish upper shed limit is grown up in the bottom, because culture dish ware body cross-section lasts to reduce, the in-process liquid feeding accessory that pushes down produces the extrusion with the vessel wall, protruding portion 120 and vessel wall through rounding produce frictional force and make protruding portion 120 and vessel wall crowded tightly, thereby near the fixed and culture dish 01 opening of liquid feeding accessory 10. At this time, the operator should apply force uniformly to make each side of the substrate 10 sink equally, and it is necessary to ensure that the edge of the substrate is lower than the opening of the culture dish, so that the culture solution flows down to prevent overflow, and the middle area of the substrate forms obvious arc bottom sample depression due to the extrusion force. The hand-held rigid tube 131 is lifted upwards, the liquid feeding fitting cannot slide due to large friction force, and an arc top-shaped bulge is formed in the middle area. Any two of the protrusions, such as the first protrusion 121 and the second protrusion 122, together with the base 110 and the wall, form a long and narrow hollow groove having a width of about 1.5 to 3mm for the culture solution to be left therebetween to enter the culture dish.

The culture solution is poured into the upper part of the base body 110 with the arc top-like bulge, and the liquid flows down along the arc top in small strands from the gaps at the periphery. Since the culture dish 01 has an inclined wall, the liquid flows down adherent. Because of the small impulse, the aggregated cells are not dispersed. The gas in the container is exhausted through the hard tube 131. The hard tube is not immersed by the culture solution due to the height, and the air exhaust is smooth.

Example 2

The culture dish kit according to the invention is used for culturing chondrocyte membranes.

As shown in fig. 4, the chondrocyte membrane sheets were cultured using the culture dish kit according to the present invention.

The culture dish 01 is an open container with a small opening at the bottom, the height is 8cm, the bottom is a rectangle of 4cm multiplied by 4cm, and the top is a rectangle opening of 6cm multiplied by 6 cm. The filling assembly 20 has a base dimension of less than about 6cm x 6cm and has a first opening 211 and a second opening 212 in the base. The first and second openings 211 and 212 are closely adjacent and define an elongated strip-like communication portion therebetween, with no rigid tubing inserted.

Chondrocytes were deposited on the bottom of the culture dish 01 for culture. The operator passes through the long and thin banded part of tweezers centre gripping liquid feeding accessory 20, put into culture dish 01 opening, because base member 210 size only slightly is less than the opening, push down earlier with the base member middle part after the in-process of pushing down, because culture dish upper shed limit is grown up in the bottom, because culture dish ware body cross-section lasts to reduce, the in-process liquid feeding accessory of pushing down produces the extrusion with the wall, produce frictional force through protrusion and the wall of rounding and make protrusion and dish wall crowded tightly, thereby near liquid feeding accessory 20 is fixed and culture dish 01 opening. At this time, the operator should apply force uniformly to make the whole sinking degree of the substrate 20 equal, and it is necessary to ensure that the edge of the substrate is lower than the opening of the culture dish, so that the culture solution flows down to prevent overflow, and the middle area of the substrate forms obvious arc bottom sample sinking due to the extrusion force. The tweezers clamp the elongated strip-shaped communication part to lift upwards, the liquid feeding accessory cannot slide due to large friction force, and the middle area forms an arc top-shaped bulge. At any two of the protrusions, for example, the first protrusion and the second protrusion, an elongated hollow groove is formed with the base 210 and the wall, and the width of the hollow groove is about 2cm for the culture solution to be left therebetween to enter the culture dish.

The culture solution is poured into the arc top-like convex substrate 210, and the liquid flows down from the gaps around the arc top in small streams and flows into the chondrocyte membrane. Because of the small impulse, the aggregated cells are not dispersed. The first and second openings 211 and 212 are used for discharging gas in the container, so that the gas can be discharged smoothly.

In order to better understand the technical solution of the present application, the following description is given with reference to a specific example, in which the listed details are mainly for the sake of understanding and are not intended to limit the scope of the present application.

It is noted that, in the present patent application, relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, the use of the verb "comprise a" to define an element does not exclude the presence of another, same element in a process, method, article, or apparatus that comprises the element. In the present patent application, if it is mentioned that a certain action is executed according to a certain element, it means that the action is executed according to at least the element, and two cases are included: performing the action based only on the element, and performing the action based on the element and other elements. The expression of a plurality of, a plurality of and the like includes 2, 2 and more than 2, more than 2 and more than 2.

This specification includes combinations of the various embodiments described herein. Separate references to "one embodiment" or a particular embodiment, etc., do not necessarily refer to the same embodiment; however, these embodiments are not mutually exclusive, unless indicated as mutually exclusive or as would be apparent to one of ordinary skill in the art. It should be noted that the term "or" is used in this specification in a non-exclusive sense unless the context clearly dictates otherwise.

All documents mentioned in this application are to be considered as being incorporated in their entirety into the disclosure of this application so as to be subject to modification as necessary. Further, it is understood that various changes or modifications may be made to the present application by those skilled in the art after reading the above disclosure of the present application, and such equivalents are also within the scope of the present application as claimed.

Claims (10)

1. A charging fitting, comprising: a base body and a plurality of projections, wherein

The substrate is sheet-shaped and is provided with one or more openings;

the protruding parts extend from the base body and are uniformly distributed on the periphery of the base body, and the protruding parts are provided with smooth arc-shaped contour lines;

the protrusion and the base are integrally formed.

2. The filling accessory of claim 1, wherein said filling accessory is made of medical silicone rubber.

3. The filling accessory of claim 1, wherein said base has an opening to which a relatively large diameter rigid tube is removably attached.

4. The filling fitting of claim 3, wherein said base has 2 openings, said 2 openings being closely adjacent and defining an elongate strip-like communication portion therebetween.

5. The filling accessory of claim 3, wherein the rigid tube has a height h, wherein h is 5-10 cm.

6. The liquid feeding accessory of claim 1, wherein the aperture of the opening is d, wherein d is in the range of 0.5-1 cm.

7. Filling accessory according to claim 1, wherein the base body is provided in a circular or rectangular shape.

8. The filling accessory of claim 1, wherein the number of protrusions is n, wherein n is from 2 to 6.

9. A culture dish kit, comprising: the filling accessory according to any one of claims 1 to 8, and

the culture dish, the culture dish is provided with the opening, the size of opening more than or equal to the size of base member makes the liquid feeding accessory can with the opening closely cooperates, base member periphery, adjacent protruding portion and culture dish inner wall restrict out the groove.

10. The culture dish kit of claim 9, wherein the culture dish has a height H, wherein H is in a range of 4-10 cm.

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