CN216274198U

CN216274198U - Closed cell culture box can be dismantled in haplopore

Info

Publication number: CN216274198U
Application number: CN202123049637.4U
Authority: CN
Inventors: 邵轶智; 徐黎明; 赵景壮; 任广明; 卢彤岩
Original assignee: Heilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences
Current assignee: Heilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences
Priority date: 2021-12-07
Filing date: 2021-12-07
Publication date: 2022-04-12
Anticipated expiration: 2031-12-07

Abstract

The utility model discloses a closed cell culture box capable of being disassembled and assembled in a single hole, which comprises a culture box body, wherein one side of the top end of the culture box body is hinged with a first box cover, supporting blocks are fixedly connected to the middle parts of the two sides of the inner wall of the culture box body, the supporting blocks are connected with a culture plate in an inserting way through connecting rods, the connecting rods are fixedly connected with the culture plate, the middle part of the culture plate is provided with six culture holes which are uniformly distributed, the culture holes are connected with culture dishes in a clamping way, the four sides of the culture holes are fixedly connected with connecting blocks, and the four sides of the culture dishes are provided with connecting grooves respectively. Avoids the evaporation of cell culture solution, saves cost and is more convenient to use.

Description

Closed cell culture box can be dismantled in haplopore

Technical Field

The utility model relates to the technical field of cell culture, in particular to a closed cell culture box with a detachable single hole.

Background

The six-hole plate for cell culture is usually used when aquatic animals detect viruses, the existing six-hole plate is a device with six holes and a cover, all the holes are exposed in the air after the cover is opened, a self-sealing bag is required to be sleeved to prevent cell culture solution in the six-hole plate from evaporating when the six-hole plate is used, only one disease can be detected, one virus is inoculated, after the virus is inoculated, the virus harvesting time is different due to possible difference of pathological change time of cells in the same six-hole plate, the situation of cross contamination or infection of cells without pathological change is easily caused when the six-hole plate cover is opened to collect the virus, the detection result is inaccurate, meanwhile, the phenomenon of cell hole waste is caused when the six-hole plate cells are not fully filled, the utilization rate of the existing six-hole plate in the market is not high, experimental errors and detection accuracy errors are easy to occur, and the humidity condition required by a culture dish can not be ensured, it is very inconvenient to use.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a closed cell culture box with a detachable single hole, which aims to solve the problems that the existing six-hole plate in the market is low in utilization rate, easy to cause experimental errors and detection accuracy errors, incapable of ensuring the humidity condition required by a culture dish and very inconvenient to use, and is provided by the background technology.

In order to achieve the purpose, the utility model provides the following technical scheme: the utility model provides a closed cell culture box can be dismantled in haplopore, includes the culture box body, one side on culture box body top articulates there is first lid, the equal fixedly connected with supporting shoe in middle part of culture box body inner wall both sides, the supporting shoe alternates through the connecting rod and is connected with the culture plate, just connecting rod and culture plate fixed connection, six evenly distributed's culture hole has been seted up at the middle part of culture plate, the culture hole block is connected with the culture dish, the equal fixedly connected with connecting block in four sides of culture hole, the spread groove has all been seted up to four sides of culture dish, the connecting block corresponds the setting with the spread groove, the top block of culture dish is connected with the second lid, water-absorbing sponge has been laid to the bottom of culture box body inner wall, the positive one side fixed mounting of culture box body has the hygrometer.

According to a preferable technical scheme of the utility model, a buckle is hinged to the middle of the front face of the first box cover, a rotating clamping block is rotatably connected to the top end of the front face of the culture box body, and the buckle is connected with the rotating clamping block in a clamping manner. The buckle and the rotary fixture block are convenient for fixing the first box cover and the culture box body, and the use is more convenient.

As a preferable technical scheme, the middle of the top end of the first box cover is hinged with a handle, and the middle of the top end of the culture box body is connected with a sealing ring in a clamping manner. The handle is convenient for take and cultivates the box body, and the sealing washer is convenient for seal between first lid and the cultivation box body.

As a preferred technical scheme of the utility model, the middle part of one side of the culture box body is fixedly communicated with a connecting pipe, one side of the inner wall of the culture box body, which is close to the connecting pipe, is fixedly provided with a liquid inlet pipe, and the liquid inlet pipe is fixedly communicated with the connecting pipe. The connecting pipe is convenient for with external liquid union coupling, and the feed liquor pipe is convenient for keep cultivateing box body internal environment moist to the inside feed liquor of cultivateing box body.

As a preferable technical scheme of the utility model, a valve is fixedly arranged in the middle of the connecting pipe. The valve is convenient for control the feed liquor of feed liquor pipe, uses more peacefully.

As a preferred technical scheme of the utility model, the middle part of the bottom end of the culture box body is fixedly provided with an antiskid pad. The non-slip mat increases the frictional force of cultivateing the box body bottom, prevents to cultivate the box body and slides, uses more relieved.

Compared with the prior art, the utility model has the beneficial effects that: through setting up first lid and second lid, its independent environment can all be guaranteed to every culture dish, makes cell culture difficult contaminated, sets up the hygrometer, and the humidity of the culture dish of being convenient for observe sets up the sponge that absorbs water and can guarantee to cultivate this internal moist of box, avoids the evaporation of cell culture liquid, practices thrift the cost, and it is more convenient to use.

Drawings

FIG. 1 is a schematic front view of the present invention;

FIG. 2 is a schematic structural view of the present invention;

FIG. 3 is a schematic view of the structure of the present invention at A;

fig. 4 is a schematic top view of the present invention.

In the figure: 1. a culture box body; 2. a first box cover; 3. a handle; 4. buckling; 5. rotating the clamping block; 6. a water-absorbing sponge; 7. culturing the plate; 8. a culture dish; 9. a second box cover; 10. a culture well; 11. connecting blocks; 12. connecting grooves; 13. a connecting rod; 14. a support block; 15. a hygrometer; 16. a connecting pipe; 17. a valve; 18. a liquid inlet pipe; 19. a non-slip mat; 20. and (5) sealing rings.

Detailed Description

The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.

Referring to fig. 1-4, the utility model provides a closed cell culture box with a detachable single hole, comprising a culture box body 1, a first box cover 2 hinged on one side of the top end of the culture box body 1, supporting blocks 14 fixedly connected with the middle parts of the two sides of the inner wall of the culture box body 1, a culture plate 7 inserted and connected with the supporting blocks 14 through connecting rods 13, the connecting rods 13 and the culture plate 7 are fixedly connected, six culture holes 10 uniformly distributed are arranged in the middle part of the culture plate 7, a culture dish 8 is clamped and connected with the culture holes 10, connecting blocks 11 are fixedly connected with the four sides of the culture holes 10, connecting grooves 12 are arranged on the four sides of the culture dish 8, the connecting blocks 11 are arranged corresponding to the connecting grooves 12, a second box cover 9 is clamped and connected with the top end of the culture dish 8, a sponge 6 is laid on the bottom end of the inner wall of the culture box body 1, a hygrometer 15 is fixedly installed on one side of the front surface of the culture box body 1, first lid 2 is convenient for sealed culture box body 1, and supporting shoe 14 is convenient for support culture plate 7, cultivates hole 10 and is convenient for support culture dish 8, and culture dish 8 is convenient for cultivate the cell, and the sponge 6 that absorbs water can guarantee to cultivate the interior moist of box, avoids the evaporation of cell culture liquid, practices thrift the cost, and the risk of pollution abatement improves the accuracy of experiment, reduces the loaded down with trivial details degree of experiment, and hygrometer 15 is convenient for observe the humidity condition of culture box body 1 inside.

Preferably, the positive middle part of first lid 2 articulates there is buckle 4, cultivates the positive top of box body 1 and rotates and be connected with and rotate fixture block 5, and buckle 4 is connected with 5 block connections of rotation fixture block, and buckle 4 uses with 5 cooperation of rotation fixture block, is convenient for fix between first lid 2 and the cultivation box body 1, and is more convenient during the use.

Preferably, the middle part on 2 tops of first lid articulates there is handle 3, cultivates the middle part block on 1 top of box body and is connected with sealing washer 20, and handle 3 is convenient for take cultivates box body 1, and sealing washer 20 is convenient for seal between to first lid 2 and cultivates box body 1.

Preferably, the middle part of cultivateing 1 one side of box body is fixed the intercommunication and has connecting pipe 16, cultivates 1 inner wall of box body and is close to the fixed feed liquor pipe 18 that is provided with in one side of connecting pipe 16, feed liquor pipe 18 and the fixed intercommunication of connecting pipe 16, connecting pipe 16 be convenient for with external liquid union coupling, feed liquor pipe 18 be convenient for to cultivateing 1 inside feed liquor of box body, keep cultivateing 1 internal environment of box body moist.

Preferably, the valve 17 is fixedly installed in the middle of the connecting pipe 16, and the valve 17 is convenient for controlling the liquid inlet of the liquid inlet pipe 18, so that the use is safer.

Preferably, the middle part of the bottom end of the culture box body 1 is fixedly provided with an anti-slip pad 19, the anti-slip pad 19 increases the friction force at the bottom end of the culture box body 1, the culture box body 1 is prevented from sliding, and the use is more relieved.

The working principle of the closed cell culture box with the detachable single hole is that one side of a first box cover 2 is hinged to enable the first box cover 2 to be used in a turnover mode, sterile double distilled water is added to a water absorption sponge 6 to keep the interior of a culture box body 1 moist, six culture dishes 8 on a culture plate 7 can be inoculated with different viruses, second box covers 9 on the six culture dishes 8 ensure the independent environment of the culture dishes 8, cell culture is not easy to pollute, a hygrometer 15 can observe the humidity condition inside the culture box body 1, when the humidity is low, a connecting pipe 16 can be connected with an external liquid pipe, a valve 17 is opened, a liquid inlet pipe 18 is used for feeding liquid, the water absorption sponge 6 absorbs liquid again to keep the humidity environment inside the culture box body 1, an anti-slip pad 19 at the bottom of the culture box body 1 increases the friction force at the bottom end of the culture box body 1 to prevent the culture box body 1 from sliding, use more relieved, when the cell in the culture dish 8 is observed to needs, open first lid 2, select the culture dish 8 that needs to observe, cross contamination's the condition can not appear in surplus culture dish 8, has reduced the risk of pollution, has improved the accuracy of experiment, has reduced the loaded down with trivial details degree of experiment.

Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes in the embodiments and/or modifications of the utility model can be made, and equivalents and modifications of some features of the utility model can be made without departing from the spirit and scope of the utility model.

Claims

1. The utility model provides a closed cell culture box can be dismantled to haplopore, includes culture box body (1), its characterized in that: the cultivation box comprises a cultivation box body (1), wherein a first box cover (2) is hinged to one side of the top end of the cultivation box body (1), supporting blocks (14) are fixedly connected to the middle portions of two sides of the inner wall of the cultivation box body (1), cultivation plates (7) are connected to the supporting blocks (14) in an inserting mode through connecting rods (13), the connecting rods (13) are fixedly connected with the cultivation plates (7), six cultivation holes (10) which are evenly distributed are formed in the middle of each cultivation plate (7), cultivation dishes (8) are connected to the cultivation holes (10) in a clamping mode, connecting blocks (11) are fixedly connected to four sides of each cultivation hole (10), connecting grooves (12) are formed in four sides of each cultivation dish (8), the connecting blocks (11) are arranged corresponding to the connecting grooves (12), a second box cover (9) is connected to the top end of each cultivation dish (8), and water absorption sponges (6) are laid at the bottom end of the inner wall of the cultivation box body (1), a hygrometer (15) is fixedly arranged on one side of the front surface of the culture box body (1).

2. The closed cell culture cassette of claim 1, wherein: the front middle part of the first box cover (2) is hinged with a buckle (4), the front top end of the culture box body (1) is rotatably connected with a rotating clamping block (5), and the buckle (4) is connected with the rotating clamping block (5) in a clamping mode.

3. The closed cell culture cassette of claim 1, wherein: the middle part on the top end of the first box cover (2) is hinged with a handle (3), and the middle part on the top end of the culture box body (1) is clamped and connected with a sealing ring (20).

4. The closed cell culture cassette of claim 1, wherein: the middle part of one side of the culture box body (1) is fixedly communicated with a connecting pipe (16), one side, close to the connecting pipe (16), of the inner wall of the culture box body (1) is fixedly provided with a liquid inlet pipe (18), and the liquid inlet pipe (18) is fixedly communicated with the connecting pipe (16).

5. The closed cell culture cassette of claim 4, wherein: and a valve (17) is fixedly arranged in the middle of the connecting pipe (16).

6. The closed cell culture cassette of claim 1, wherein: the middle part of the bottom end of the culture box body (1) is fixedly provided with an anti-slip pad (19).