CN216274189U - Cell culture bottle with wide opening - Google Patents

Abstract

The utility model discloses a cell culture bottle with a wide opening, and mainly relates to a cell culture bottle. The bottle comprises a bottle body and a bottle cap, wherein the bottle cap seals and seals a top pipe orifice of the bottle body; the top pipe orifice of the bottle body is a flexible plastic pipe orifice capable of being pinched and deformed, the top of the soft plastic pipe orifice after being pinched and deformed is elliptical, the bottom of the flexible plastic pipe orifice is circular, and a deformable transitional pipe wall is arranged between the top of the elliptical pipe orifice and the bottom of the circular pipe orifice; the soft plastic pipe orifice can restore to be deformed into a round pipe orifice in an unstressed state after being pinched and deformed. The utility model has the beneficial effects that: the pipette tip feeding angle can be increased to a certain degree, so that the tail end of the pipette tip of the cell pipettor easily reaches the bottom surface included angle of the bottom of the culture bottle, when the pipette sucks the last small amount of liquid, the cell culture bottle is inclined, the culture liquid is converged to one of the bottom surface included angle positions, and complete and maximum pipette suction is carried out through the tail end of the pipette tip of the cell pipettor.

Description

A wide-mouth cell culture flask

technical field

The utility model relates to a cell culture bottle, in particular to a wide-mouth cell culture bottle.

Background technique

As shown in Figure 5 and Figure 6 of the accompanying drawings, in the cell culture experiment, the bottle mouth of the traditional culture bottle is cylindrical and small, and the tip of the cell pipette can only extend into the center of the bottom surface of the culture bottle at most. Pipetting is carried out within the range of length L in Figure 6 of the accompanying drawings, and it is difficult to reach the corner of the bottom surface of the culture flask, that is, the tip of the cell pipette is affected by the upper inner wall of the top nozzle (marked at 9), the top tube The limit restriction of the inner wall (mark 10) under the mouth, the cell pipette tip cannot be tilted excessively, resulting in the left and right sides within the range of the length L of the bottom surface of the culture bottle (that is, the range of lengths L1 and L2 in Figure 6 of the accompanying drawings). range) cannot be touched. Because the bottom surface of the cell culture flask is slightly convex, the middle is high and the surrounding area is short, the culture medium along the edge of the bottom surface of the cell culture flask cannot be absorbed.

Since the cells cultured in the culture medium have different concentrations in each layer, when pipetting the pipette tip of the cell pipette, it is necessary to pipette all the culture medium in the culture flask as much as possible to facilitate subsequent studies such as concentration analysis and measurement. Therefore, if all the culture medium samples cannot be pipetted, it will lead to subsequent analysis errors, and it is impossible to pour out the culture medium from the bottle mouth due to the sticky wall of the culture medium.

Utility model content

The purpose of this utility model is to provide a wide-mouthed cell culture bottle, which is innovatively designed as a brand-new "wide-mouthed" culture bottle, which can increase the angle of the pipette tip to a certain extent, so that the cell pipette can The end of the pipette tip can easily reach the bottom angle of the bottom surface of the culture flask. When the last small amount of liquid is pipetted, tilt the cell culture flask to make the culture liquid flow to one of the bottom angle positions. , Maximum pipetting.

In order to realize the above-mentioned purpose, the present utility model realizes through the following technical solutions:

A wide-mouth cell culture bottle, comprising a bottle body and a bottle cap, the bottle cap seals and blocks the top nozzle of the bottle body; the top nozzle of the bottle body is a soft plastic nozzle that can be squeezed and deformed, The top of the soft plastic nozzle after squeezing and deformation is elliptical, the bottom of the soft plastic nozzle is circular, and a deformable transition pipe wall is formed between the top of the elliptical nozzle and the bottom of the circular nozzle; the soft plastic After the nozzle is pinched and deformed, it can be restored to a circular tubular nozzle in a non-stressed state.

The bottle cap is an externally threaded cap, and the inner wall of the top nozzle is an internal thread; after the soft plastic nozzle is reshaped into a round tubular nozzle, the externally threaded cap is connected with the internal thread to contact and seal.

The bottle cap is a rubber stopper, and the rubber stopper is inserted into the deformed circular tube-shaped top nozzle and then contacts and seals with the inner wall of the tube.

The outer side wall of the top nozzle is integrally injection-molded with anti-skid bumps.

The top of the bottle body is provided with an opening, and the bottom of the top nozzle is thermoplastically welded at the opening.

Compared with the prior art, the beneficial effects of the present utility model are:

1. It is innovatively designed to be a brand-new "wide-mouth" culture bottle, so that the inner wall of the top nozzle is flared into an inclined surface, as shown in Figure 3 in the accompanying drawing, so that the top of the top nozzle is elliptical, and the top of the nozzle is elliptical. The upper and inner side walls of the top nozzle that are in contact with the side of the pipette tip expand outward. When the side of the pipette tip extends in, it is in contact with the inclined surface, so that the angle of the pipette tip entering the gun can be increased to a certain extent, so that the cells can be moved. The end of the pipette tip can easily reach the bottom angle of the bottom of the culture bottle. When the last small amount of liquid is sucked, tilt the cell culture bottle, as shown in Figure 4 in the accompanying drawing, so that the culture fluid flows to one of the bottom angles. Complete, maximum pipetting through the tip of the cell pipette tip.

2. The setting of the anti-skid bumps on the outer side wall of the top nozzle is to play an anti-skid effect when squeezing the top nozzle.

3. The bottle body is made of hard plastic, and the top nozzle is made of soft plastic. The two materials are different, so that the cell culture flask cannot be integrally injection molded. In order to facilitate production, the bottom of the top nozzle is set at the opening by thermoplastic welding. .

Description of drawings

Accompanying drawing 1 is the use structure schematic diagram of Embodiment 1 of the present invention.

Figure 2 is a schematic diagram of the use structure of the second embodiment of the present invention.

FIG. 3 is a schematic cross-sectional view of the use structure of Embodiment 1 of the present invention.

Figure 4 is a state diagram of the utility model in use.

FIG. 5 is a schematic diagram of the use structure of the existing cell culture flask.

Figure 6 is a schematic cross-sectional view of the structure of the existing cell culture flask in use.

Symbols shown in the attached drawings:

1. Bottle body; 2. Bottle cap; 3. Top nozzle; 4. External thread cap; 5. Internal thread; 6. Rubber stopper; 7. Anti-slip bumps; 8. Opening; 9. Upper inner wall of top nozzle; 10. The lower inner wall of the top nozzle; 11. The inclined surface; 12. The included angle of the bottom surface.

Detailed ways

The present utility model will be further described below in conjunction with specific embodiments. It should be understood that these embodiments are only used to illustrate the present invention and not to limit the scope of the present invention. In addition, it should be understood that after reading the teaching content of the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the limited scope of the present application.

The utility model is a wide-mouth cell culture bottle, the main structure includes a bottle body 1 and a bottle cap 2, and the bottle cap 2 seals and blocks the top nozzle 3 of the bottle body 1; the main improvement and optimization of the technology The design points are technical improvements in the following areas:

The top nozzle 3 of the bottle body 1 is a soft plastic nozzle that can be squeezed and deformed, an opening 8 is provided on the top of the bottle body 1 , and the bottom of the top nozzle 3 is thermoplastically welded at the opening 8 . The bottle body 1 is made of hard plastic, and the top nozzle 3 is made of soft plastic. The materials of the two are different, so that the cell culture flask cannot be integrally injection molded. In order to facilitate production, the bottom of the top nozzle 3 is set at the opening by thermoplastic welding. 8 places.

As shown in Figure 1, Figure 2, Figure 3, Figure 4 of the accompanying drawings, the top of the soft plastic nozzle after pinching and deformation is oval, while the bottom of the soft plastic nozzle cannot be deformed due to the limitation of the shape and size of the opening 8. It is a circular shape, and a deformable transition tube wall is formed between the top of the elliptical orifice and the bottom of the circular orifice; the shape after the overall pinching and deformation is shown in detail in the enlarged view in FIG. 4 of the accompanying drawings.

The outer side wall of the top nozzle 3 is integrally injection-molded with anti-skid bumps 7 . The anti-skid bumps 7 are provided to play an anti-skid function when the top nozzle 3 is squeezed.

When the culture bottle needs to be sealed, the soft plastic nozzle can be restored to a non-stressed state after being squeezed and deformed.

In summary:

As shown in Fig. 1, Fig. 2, Fig. 3, and Fig. 4 of the accompanying drawings, the device is innovatively designed as a brand-new "wide-mouth" culture bottle, so that the inner wall of the top nozzle 3 is flared into an inclined surface 11, so that the top pipe The top of the nozzle of port 3 is elliptical, and the upper and inner side walls 9 of the top nozzle that are in contact with the side of the pipette tip expand outward. When the side of the pipette tip extends in, it is in contact with the inclined surface 11. The wall 10 is in contact with the side of the pipette tip on the side away from the upper inner side wall, so as to limit the feeding angle, which can increase the feeding angle of the pipette tip to a certain extent, so that the end of the cell pipette tip can easily reach 12 at the included angle of the bottom surface of the bottom surface of the culture flask. When the last small amount of liquid is pipetted, tilt the cell culture flask, as shown in Figure 4 of the accompanying drawings, so that the culture fluid flows to one of the included angle positions on the bottom surface. The tip of the head performs complete, maximum pipetting.

As shown in Figure 6 of the accompanying drawings, the angle of entry of the pipette tip of the existing cell culture flask is a, and the angle of entry of the pipette tip of the cell culture flask improved by this patent is b, which can be known by obvious comparison: The patented technology increases the entry angle of the pipette tip to a certain extent, so that the end of the tip of the positive cell pipette can easily reach the included angle of the bottom surface of the culture flask 12 .

There are two embodiments for the sealing connection between the bottle cap 2 and the top nozzle 3, as follows:

Example 1:

The bottle cap 2 is an externally threaded cap 4, and the inner wall of the top nozzle 3 is an internal thread 5; after the soft plastic nozzle is reshaped into a round tubular nozzle, the externally threaded cap 4 is connected to the internal thread 5 to contact and seal. .

Example 2:

The bottle cap 2 is a rubber stopper 6, and the rubber stopper 6 is inserted into the deformed circular tubular top nozzle 3 and then contacts and seals with the inner wall of the tube.

Claims (5)

1. A wide-mouthed cell culture bottle, comprising a bottle body (1) and a bottle cap (2), and the bottle cap (2) seals the top nozzle (3) of the bottle body (1); it is characterized in in: The top nozzle (3) of the bottle body (1) is a soft plastic nozzle that can be squeezed and deformed. Between the top of the nozzle and the bottom of the round nozzle is a deformable transition wall; After the soft plastic nozzle is deformed by pinching, it can be restored to a circular tubular nozzle in a non-stressed state. 2. A kind of wide-mouthed cell culture flask according to claim 1 is characterized in that: the bottle cap (2) is an external thread cap head (4), and the inner wall of the top nozzle (3) is an internal thread ( 5); After the soft plastic nozzle is reshaped into a round tubular nozzle, the outer thread cap head (4) is connected to the inner thread (5) for contact and sealing. 3. A kind of wide mouth cell culture flask according to claim 1, is characterized in that: described bottle cap (2) is rubber stopper (6), and described rubber stopper (6) is inserted into the circular tubular top that restores deformation After the nozzle (3) is in contact with the inner wall of the pipe, it is sealed. 4. A wide-mouth cell culture flask according to claim 2 or 3, characterized in that: the outer side wall of the top nozzle (3) is integrally injection-molded with anti-skid bumps (7). 5. A wide-mouthed cell culture bottle according to claim 4, characterized in that: an opening (8) is provided at the top of the bottle body (1), and the bottom of the top nozzle (3) is thermoplastically welded on the opening (8).

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