CN216270461U - Cell freeze thawing liquid holding vessel - Google Patents

Abstract

The utility model relates to the technical field of vaccine production equipment, in particular to a cell freezing and thawing liquid storage tank which comprises a tank body, a sealing cover and a constant pressure device for enabling the external pressure in the cell freezing and thawing liquid storage tank to be equal, wherein a liquid inlet is formed in the tank body or the sealing cover, a switch cover is arranged on the liquid inlet, and a liquid outlet joint for connecting a liquid outlet pipe is arranged below the tank body. Through set up the constant voltage equipment that is used for making the interior external pressure of cell freeze thawing liquid holding vessel equal on jar body or sealed lid to set up the inlet that is used for pouring into the freeze thawing liquid on jar body or sealed lid, and set up the switch lid in inlet department, set up the play liquid joint that is used for connecting the drain pipe in jar body below and be used for freezing the outflow of thawing liquid, because constant voltage equipment's setting and the gravity action of freezing thawing liquid, make freeze thawing liquid can flow smoothly from going out liquid joint and be used for mixing with semi-manufactured goods and close the liquid. The device can reduce the contact probability of the cell suspension and the surrounding environment in the liquid mixing process, thereby reducing the probability of pollution in the liquid mixing process.

Description

Cell freeze thawing liquid holding vessel

Technical Field

The utility model relates to the technical field of vaccine production equipment, in particular to a cell freezing and thawing liquid storage tank.

Background

The porcine epidemic diarrhea is a contact intestinal infectious disease, and experiments prove that the average feed intake of a week before diarrhea is between 2.0g and 4.0kg, the average feed intake of the diarrhea stage is between 0.8 and 1.2kg, and the homologous ratio is reduced by about 65 percent. Therefore, the diarrhea has a large influence on the feed intake of the pigs, the reduction of the feed intake can influence the daily gain of the pigs, the average daily gain of the pigs in the week before the diarrhea is 350g-1200g, the average daily gain of the pigs in the diarrhea period is minus 300 g-minus 100g, and the average daily gain is reduced by 90-190 percent. Therefore, the use of porcine epidemic diarrhea vaccines is particularly important for prevention and control.

The production of the porcine epidemic diarrhea vaccine comprises seed virus culture and seed cell culture, inoculating VERO cell suspension to a cell factory for adherent culture and passage after the seed cell culture, preparing into a semi-finished product after virus inoculation, freeze thawing, liquid combination, inactivation and filtration, and finally canning. In the process of mixing and inactivating the cell suspension in the cell factory by pouring the cell suspension in a beaker and pumping the cell suspension into a semi-finished product barrel by using a peristaltic pump, the probability of pollution of the cell suspension is high because the opening of the beaker is too large and the contact area with the environment is large.

Therefore, the problem to be solved by the technical staff in the field is to provide a storage tank for cell freezing and thawing solution, which can reduce the contact probability of the cell suspension and the environment during the solution mixing process and further reduce the probability of the cell suspension being polluted during the solution mixing process.

SUMMERY OF THE UTILITY MODEL

In order to solve the technical problem, the utility model provides a cell freezing and thawing liquid storage tank.

In order to achieve the purpose, the utility model provides the following technical scheme:

the utility model provides a cell freeze thawing liquid holding vessel, includes a jar body and sealed lid, still includes the constant voltage equipment who is used for making the interior external pressure of cell freeze thawing liquid holding vessel equal, jar body or sealed covering are equipped with the inlet, the inlet is equipped with the switch cover, jar body below is equipped with the play liquid joint that is used for connecting the drain pipe.

Preferably, a sealing ring is arranged between the tank body and the sealing cover.

Preferably, the sealing cover further comprises a disk hoop or a hoop used for clamping the sealing cover and/or the switch cover.

Preferably, the constant pressure device is an air filtering device which enables the inside and the outside of the tank body to be communicated.

Preferably, the constant pressure device is provided on the sealing cover or the tank body.

Preferably, the air filter device is provided with a breathable membrane for the circulation of air.

Preferably, the material of the breathable membrane is ePTFE.

Preferably, the outer wall of the tank body is provided with two handles.

Compared with the prior art, the utility model has the technical effects that:

through set up the constant voltage equipment that is used for making the interior external pressure of cell freeze thawing liquid holding vessel equal on jar body or sealed lid to set up the inlet that is used for pouring into the freeze thawing liquid on jar body or sealed lid, and set up the switch lid in inlet department, set up the play liquid joint that is used for connecting the drain pipe in jar body below and be used for freezing the outflow of thawing liquid, because constant voltage equipment's setting and the gravity action of freezing thawing liquid, make freeze thawing liquid can flow smoothly from going out liquid joint and be used for mixing with semi-manufactured goods and close the liquid. The device can reduce the contact probability of the cell suspension and the surrounding environment in the liquid mixing process, thereby reducing the probability of pollution in the liquid mixing process.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a schematic view of the structure of the present invention.

Detailed Description

Technical terms or scientific terms used in the present specification should be given their ordinary meanings as understood by those skilled in the art: the use of "first," "second," and the like in this description does not denote any order, quantity, or importance, but rather the terms "first," "second," and the like are used to distinguish one element from another; the terms "connected" or "communicating" and the like are not restricted to physical or mechanical connections, but may include various forms of connections, whether direct or indirect; "upper", "lower", "left", "right", "front", "rear", "far", "near", "start", "end", and the like are used only to indicate relative positional relationships, and when the absolute position of a described object is changed, the relative positional relationships may also be changed accordingly; in addition, in the description of the present specification, the meaning of "a plurality" or "several" is two or more unless otherwise specified.

For a better understanding of the present invention, embodiments thereof will be described below with reference to the accompanying drawings.

As shown in figure 1, a cell freeze thawing liquid holding vessel, including jar body 1 and sealed lid 2, still including being used for making the constant voltage equipment 3 that the internal and external pressure of cell freeze thawing liquid holding vessel equals, be equipped with inlet 4 on jar body 1 or sealed lid 2, for the ease of pouring liquid, set up the shape of inlet into the funnel shape, still set up the switch lid in inlet 4 department for preventing that impurity from getting into to cause the pollution, jar body 1 below is equipped with the play liquid joint 5 that is used for connecting the drain pipe, here connects for pagoda joint, the position that goes out the liquid joint is best with the bottom parallel and level of the jar body. When the tank body is manufactured specifically, the inner wall and the outer surface of the tank body can be smooth and flat so as to prevent liquid from remaining and being incompletely cleaned, and the tank body and the sealing cover can be made of medical stainless steel 316.

In order to further keep the inside of the tank body sealed, a sealing ring is arranged between the tank body 1 and the sealing cover 2, wherein the sealing ring used in the process is a medical high-temperature-resistant (the high temperature refers to more than 100 ℃) sealing ring; meanwhile, a disc hoop 6 or a hoop for tightening the sealing cover 2 and/or the switch cover is also arranged, specifically, the sealing cover 2 and the tank body 1 can be buckled, the disc hoop 6 is arranged at the edge formed after buckling and tightened, so that the sealing cover 2 and the tank body 1 cannot be separated under the condition that the disc hoop 6 is not taken down, and the closing and tightening of the switch cover at the liquid inlet can also adopt the same structure.

The constant pressure device 3 is an air filter device for communicating the inside and the outside of the tank 1, and can be arranged on the sealing cover 2 and also on the tank 1, and in a specific embodiment, the air filter device is arranged on the sealing cover 2, and the specific connection mode is as follows: the sealed tank body is kept in constant pressure inside and outside by arranging the two-way joint on the sealing cover 2, wherein the joint can be a pagoda joint, and then the air filtering device is connected to the joint by using the silicone tube.

The air filtering device is not required to be of a complex structure, and can be a silicone tube, a silicone disc is arranged at a certain position of the silicone tube, a circular breathable film for air circulation is arranged in the disc, and the breathable film is made of ePTFE (expanded polyethylene terephthalate), so that a good waterproof, dustproof and breathable effect can be achieved; the air filter device can also be a common air filter element, and the air filter is directly arranged at the two-way joint.

In another embodiment, two handles 7 are provided on the outer wall of the tank 1 for easy carrying or taking.

The cell freezing and thawing liquid storage tank has the following use process: before use, the switch cover is disinfected, the silicone tube is connected to the liquid outlet joint, and the tail end of the silicone tube is wrapped by tinfoil, so that the step of installing the silicone tube again is omitted after disinfection to prevent pollution; at the moment, the cell freezing and thawing solution storage tank can be placed in a high-temperature and high-pressure environment for disinfection, and the cell freezing and thawing solution storage tank can be used after being cooled after disinfection. When the device is used, the working environment is ensured to be an aseptic environment, after the cell liquid freezing and thawing of a cell factory is finished, the switch cover of the cell freezing and thawing liquid storage tank is opened, the freezing and thawing liquid of the cell factory is filled from the liquid inlet 4, and finally, due to the arrangement of the constant pressure device and the gravity action of the freezing and thawing liquid, the freezing and thawing liquid in the tank body 1 can smoothly pass through the liquid outlet joint 5 and flow out through the liquid outlet pipe to enter the half-mouth barrel for liquid combination, and the freezing and thawing liquid can also flow out from the liquid outlet joint 5 rapidly by using the assistance of a peristaltic pump in the process; when the freeze-thaw liquid flows out of the tank body, in order to prevent the freeze-thaw liquid from flowing back, a one-way valve is arranged on the liquid outlet pipe, compared with a beaker with a large caliber, the liquid outlet pipe connected with the liquid outlet joint 5 of the device has a thinner pipe diameter and can directly extend into a semi-finished product barrel, compared with the use of the beaker, the pollution probability of the cell freeze-thaw liquid storage tank after disinfection is smaller, and the liquid inlet can also be set to be with a smaller caliber when the freeze-thaw liquid is poured, so that the freeze-thaw liquid temporarily stored in the tank body can be prevented from being polluted; when the liquid is combined, the liquid outlet pipe is directly extended into the semi-finished product mixing barrel, so that the contact probability of the cell suspension and the surrounding environment in the liquid combining process is reduced, and the probability of pollution in the liquid combining process is further reduced.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various changes and modifications can be made without departing from the overall concept of the present invention, and these should also be considered as the protection scope of the present invention.

Claims (9)

1. The utility model provides a cell freeze and melt liquid holding vessel, includes jar body (1) and sealed lid (2), its characterized in that: still including being used for making constant voltage equipment (3) that the interior external pressure of cell freeze thawing liquid holding vessel equals, be equipped with inlet (4) on jar body (1) or sealed lid (2), inlet (4) are equipped with the switch lid, jar body (1) below is equipped with play liquid joint (5) that are used for connecting the drain pipe.

2. The storage tank for cell freezing and thawing solution as claimed in claim 1, wherein: and a sealing ring is arranged between the tank body (1) and the sealing cover (2).

3. The storage tank for cell freezing and thawing solution as claimed in claim 1, wherein: the sealing cover further comprises a disc hoop (6) or a hoop for clamping the sealing cover (2) and/or the switch cover.

4. The storage tank for cell freezing and thawing solution as claimed in claim 1, wherein: the constant pressure device (3) is an air filtering device which enables the inside and the outside of the tank body (1) to be communicated.

5. The storage tank for cell freezing and thawing solution as claimed in claim 4, wherein: the constant pressure device is arranged on the sealing cover (2) or the tank body (1).

6. The storage tank for cell freezing and thawing solution as claimed in claim 4, wherein: the air filtering device is provided with a breathable film for air circulation.

7. The storage tank for cell freezing and thawing solution as claimed in claim 6, wherein: the breathable membrane is made of ePTFE.

8. The storage tank for cell freezing and thawing solution as claimed in claim 1, wherein: the outer wall of the tank body (1) is provided with two handles (7).

9. The storage tank for cell freezing and thawing solution as claimed in claim 1, wherein: the liquid outlet joint (5) is a pagoda joint.

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