CN216248000U

CN216248000U - H5 type avian influenza detection kit

Info

Publication number: CN216248000U
Application number: CN202122772246.9U
Authority: CN
Inventors: 杨亮亮; 王文霞
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-11-12
Filing date: 2021-11-12
Publication date: 2022-04-08
Anticipated expiration: 2031-11-12

Abstract

The utility model relates to an H5 avian influenza detection kit, which comprises: a sampling tool, a suction pipe and a detection card; the sampling tool comprises a sampling swab or a cotton swab, and further comprises a sampling pipe, wherein an eluent is filled in the sampling pipe; the detection card comprises a shell and detection test paper, and the detection test paper is positioned in the shell; the detection test paper comprises a colloidal gold combination pad, the colloidal gold combination pad contains colloidal gold coupled with an anti-avian influenza antibody, the detection test paper is provided with a test line and a quality control line, the test line is coated with an avian influenza antibody with H5 type specificity in advance, and the quality control line is coated with a quality control line antibody in advance; the shell is provided with a sample adding window and an observation window; the observation window is used for observing the change conditions of the test line and the quality control line before and after detection. The kit can meet the requirement of detecting the H5 avian influenza, for example, the kit is used for detecting the H5 avian influenza virus in saliva, tissues, excrement and other samples, and has the advantages of simple operation, accurate and rapid detection result and the like.

Description

H5 type avian influenza detection kit

Technical Field

The utility model belongs to the technical field of kits, and particularly relates to an H5 avian influenza detection kit.

Background

Avian Influenza (AI) is an avian (poultry or wild bird, as well as some mammals) infectious disease caused by influenza a viruses of the family orthomyxoviridae, genus influenzae. The H5 avian influenza is one type of avian influenza, is caused by influenza A virus, belongs to poultry virulent infectious diseases, is classified as a type of animal epidemic diseases in China, and has the characteristics of short incubation period, quick morbidity, high disease mortality and the like. The current common methods for detecting avian influenza virus comprise a chick embryo virus separation method, a fluorescence PCR method and the like. The chicken embryo virus separation method has high technical requirements and long time consumption; the fluorescent PCR method requires special equipment and instruments to complete the detection.

The rapid monitoring of the avian influenza has important significance for epidemic prevention and control, so that the provision of the rapid avian influenza kit which is simple to operate and has low technical requirements is particularly necessary.

SUMMERY OF THE UTILITY MODEL

In view of the problems in the prior art, the utility model provides the H5 avian influenza detection kit, which can meet the requirement of quickly detecting the H5 avian influenza, does not need complex equipment, and is simple to operate and easy to operate by personnel.

The technical scheme for solving the technical problems is as follows:

an avian influenza H5 detection kit, comprising: a sampling tool, a suction pipe and a detection card; the sampling tool comprises a sampling swab or a cotton swab; the sampling tool also comprises a sampling pipe, and eluent is filled in the sampling pipe; the detection card comprises a shell and detection test paper, and the detection test paper is positioned in the shell; the detection test paper comprises a sample pad, a colloidal gold combined pad, a chromatographic membrane and a water absorption pad, wherein one end of the sample pad is connected with one end of the colloidal gold combined pad, the other end of the colloidal gold combined pad is connected with one end of the chromatographic membrane, and the other end of the chromatographic membrane is connected with one end of the water absorption pad; a test line and a quality control line are arranged at the colloidal gold bonding pad; the shell is provided with a sample adding window and an observation window, the sample adding window is used for adding a sample to be detected, and the observation window is used for observing the change conditions of the test line and the quality control line before and after detection.

Adopt above-mentioned technical scheme's beneficial effect to include:

after a sample to be detected is collected by using a sampling swab or a cotton swab, the sample to be detected is placed in a sampling tube to be mixed with an eluent, a straw is used for sucking the mixed liquid to be dripped at a sample adding window of a detection card, if avian influenza antigen exists in the sample, the color development of a test line and a quality control line can be observed at the observation window, and therefore the purpose of rapid and accurate detection is achieved.

The kit can meet the requirement of detecting H5 avian influenza, and is used for detecting H5 avian influenza virus in saliva, tissues, excrement and other samples. The method has the advantages of simple operation, no need of complex equipment, low requirement on operators, easy operation, accurate and rapid detection result, strong specificity, good sensitivity and the like.

The technical scheme of the utility model can be further improved as follows.

Furthermore, a sample can be added to the sample pad through the sample adding window of the shell, and the change conditions of the test line and the quality control line before and after detection can be observed through the observation window; the test line and the quality control line are made of nitrocellulose membrane strips.

Adopt above-mentioned technical scheme's beneficial effect to include: the structure can realize the detection of H5 avian influenza through chromatography.

Further, the straw is hollow inside, and comprises a first tubular part, a second tubular part, a third tubular part and a fourth tubular part; one end of the first tubular member is communicated with one end of the second tubular member, the other end of the second tubular member is communicated with one end of the third tubular member, and the other end of the third tubular member is communicated with one end of the fourth tubular member; the other end of the first tubular component is closed, and the other end of the fourth tubular component is open and used for sucking a sample to be detected.

Adopt above-mentioned technical scheme's beneficial effect to include: the first tubular part is used for being held by hands to be convenient to take, the second tubular part has a buffering effect, and the fourth tubular part is used for sucking a sample to be detected.

Further, the pipe diameter of the first tubular member is smaller than that of the second tubular member, the pipe diameter of the second tubular member is larger than that of the third tubular member, and the pipe diameters of the first tubular member, the second tubular member and the third tubular member are all larger than that of the fourth tubular member.

Adopt above-mentioned technical scheme's beneficial effect to include: the pipe diameter of the second tubular part is larger than the pipe diameters of other parts, so that the buffer effect is good, and a suction pipe can suck a sample; the smaller diameter of the fourth tubular member is advantageous for sucking a smaller volume of sample.

Further, the first tubular member, the second tubular member, the third tubular member and the fourth tubular member are integrally formed to constitute the straw.

Further, the colloidal gold conjugate pad contains colloidal gold coupled with an anti-avian influenza antibody; the test line is pre-coated with an H5 type specific avian influenza antibody, and the quality control line is pre-coated with a quality control line antibody.

Adopt above-mentioned technical scheme's beneficial effect to include: the detection test paper contains colloidal gold coupled with anti-avian influenza antibody, and is provided with a test line and a quality control line, wherein the test line is coated with the avian influenza antibody with H5 type specificity in advance, if the avian influenza antigen exists in the sample, the color development of the test line and the quality control line can be observed at the observation window, so that the purpose of rapid and accurate detection is realized.

Further, the sampling tool also includes sterile gloves, masks, goggles, scissors, markers, sample bags, and a timer.

Adopt above-mentioned technical scheme's beneficial effect to include: the sterile gloves, the mask and the goggles can meet the requirement of sterile operation so as to avoid infection; the scissors are convenient to cut a sample to be detected; the collected sample can be marked by a marking pen after being put into a sample bag, so that the sample is prevented from being mixed; the timer is used for recording and reminding the reaction time, and inaccurate judgment of the detection result caused by overlong or overlong reaction time is avoided.

Further, the area of the observation window is larger than that of the sample adding window.

Adopt above-mentioned technical scheme's beneficial effect to include: the small area of the sample adding window can avoid pollution caused by excessive exposure, and the large area of the observation window is favorable for observing a detection result.

Further, the distance between the sample adding window and the test line is smaller than the distance between the sample adding window and the quality control line.

Furthermore, the straw is made of plastic; the straw is also provided with scale marks.

Adopt above-mentioned technical scheme's beneficial effect to include: the graduation marks of the pipette facilitate the aspiration of the desired amount of sample.

Further, the sampling pipe includes tube cap and body, and tube cap detachably sets up in the mouth of pipe department of body, contains the eluant in the body.

Adopt above-mentioned technical scheme's beneficial effect to include: the sampling pipe of area lid is favorable to sealing the body, is convenient for rock operations such as vibrate.

Drawings

FIG. 1 is a schematic structural diagram of an avian influenza H5 detection kit;

FIG. 2 is a schematic diagram of a test card;

FIG. 3 is a schematic view of the structure of the straw;

FIG. 4 is a schematic diagram of the detection result after the detection is performed by using the H5 avian influenza detection kit;

in the drawings, wherein the various reference numbers represent the following list of elements:

1. the kit comprises a sampling tool, 2, a pipette, 21, a first tubular part, 22, a second tubular part, 23, a third tubular part, 24, a fourth tubular part, 3, a detection card, 31, a shell, 32, a test line, 33, a quality control line, 34, a sample pad, 35, a colloidal gold binding pad, 36, a chromatographic membrane, 37 and a water absorption pad.

Detailed Description

The principles and features of this invention are described below in conjunction with the following drawings, which are set forth by way of illustration only and are not intended to limit the scope of the utility model.

As shown in figure 1, the utility model provides an H5 avian influenza detection kit, which comprises a sampling tool 1, a suction tube 2 and a detection card 3. The sampling tool 1 is used for collecting a sample to be detected, the suction pipe 2 is used for sample adding, and the detection card 3 is a main component for detecting the H5 avian influenza.

Sampling instrument 1 is including sampling swab or cotton swab, a sample for the collection waits to detect, can also include the sampling pipe, aseptic gloves, the gauze mask, the goggles, the scissors, the marker pen, sample bag and timer, aseptic gloves, the gauze mask, thereby the goggles can satisfy aseptic operation's requirement and avoid infecting, the scissors conveniently is cut and is waited the sample of detecting, the sample after the collection can be marked with the marker pen after packing into the sample bag, avoid making and mix, the timer is used for the time of record and warning reaction, avoid judging inaccurately because of reaction time overlength or short detection result that leads to. The sampling pipe is equipped with the eluant in and is used for mixing with the sample that waits to detect, and the material of sampling pipe can be transparent plastic, is convenient for observe the state of intraductal sample, and the sampling pipe includes tube cap and body, contains the eluant in the body and the body has the scale mark and is used for the sign volume, and tube cap detachably sets up in the mouth of pipe department of body, and the mouth of pipe can be equipped with the screw thread, and the tube cap passes through the screw thread and is connected with the body.

As shown in fig. 2, the detection card 3 includes a housing 31 and a detection test paper, the detection test paper is located in the housing 31, and the housing 31 may be made of plastic; the test paper includes sample pad 34, colloidal gold combination pad 35, chromatographic membrane 36 and water absorption pad 37, and the one end of sample pad 34 is connected with the one end of colloidal gold combination pad 35, and the other end of colloidal gold combination pad 35 is connected with the one end of chromatographic membrane 36, and the other end of chromatographic membrane 36 is connected with the one end of water absorption pad 37, can select concrete connection's mode according to actual need, for example: lap joint and the like; the test line 32 and the quality control line 33 are located at the colloidal gold bonding pad 35, and the test line 32 and the quality control line 33 are made of nitrocellulose membrane strips.

The shell 31 is provided with a sample adding window and an observation window, the sample adding window is used for adding a sample to be detected, and the observation window is used for observing the change conditions of the test line 32 and the quality control line 33 before and after detection. The distance between the loading window and the test line 32 is smaller than the distance between the loading window and the quality control line 33. The sample can be added to the sample pad 34 through the sample adding window of the housing 31, and the change of the test line 32 and the quality control line 33 before and after the detection can be observed through the observation window. The area of the observation window is larger than that of the sample adding window.

The colloidal gold combined pad 35 contains colloidal gold coupled with anti-avian influenza antibody, the detection test paper is provided with a test line 32 and a quality control line 33, the test line 32 is coated with the avian influenza antibody with H5 type specificity in advance, and the quality control line 33 is coated with the quality control line antibody in advance.

As shown in fig. 3, the straw 2 is hollow inside and includes a first tubular member 21, a second tubular member 22, a third tubular member 23, and a fourth tubular member 24; one end of the first tubular member 21 communicates with one end of the second tubular member 22, the other end of the second tubular member 22 communicates with one end of the third tubular member 23, and the other end of the third tubular member 23 communicates with one end of the fourth tubular member 24; the other end of the first tubular member 21 is closed, and the other end of the fourth tubular member 24 is open for sucking a sample to be tested.

The tube diameter of the first tubular member 21 is smaller than the tube diameter of the second tubular member 22, the tube diameter of the second tubular member 22 is larger than the tube diameter of the third tubular member 23, and the tube diameters of the first tubular member 21, the second tubular member 22, and the third tubular member 23 are all larger than the tube diameter of the fourth tubular member 24. The pipe diameter of the second tubular part 22 is larger than the pipe diameters of other parts, so that the buffer effect is good, and the suction pipe 2 can suck samples conveniently; the smaller tube diameter of the fourth tubular member 24 is advantageous for sucking a smaller volume of sample.

The first tubular member 21, the second tubular member 22, the third tubular member 23, and the fourth tubular member 24 are integrally formed to constitute the straw 2. The straw 2 can be made of plastic, the shape of the straw can be changed under the action of force, and the straw 2 can be provided with scale marks.

The kit is prepared by utilizing an immunochromatography technology. The kit comprises a colloidal gold combination pad 35 containing colloidal gold (antibody conjugate) coupled by an anti-avian influenza antibody, a test line 32 (T line for short) and a quality control line 33 (C line for short), wherein the T line and the C line are made of nitrocellulose membranes. The T line is coated with specific avian influenza antibody in advance, and the C line is coated with quality control line antibody in advance.

After the sample is collected, the sample is transferred to a sample treatment solution or an eluent matched with the detection card 3, and the sample treatment solution or the eluent can be water or other liquids. After the treated sample is added to the loading window, the sample moves on the test paper by capillary action. If the H5 avian influenza antigen exists in the added sample, the antigen is combined with the antibody conjugate to form an immune complex, and then the immune complex is captured on a membrane by the coated anti-H5 avian influenza antibody to enable a T line to develop color, thereby indicating that the avian influenza detection result is positive. The line C is internal quality control, and after the test sample is added, a normally-developed line C strip appears after 1 min. If the C line does not develop color, the test result is invalid, and the detection card must be replaced to retest the sample.

The following description is given by way of specific examples.

The use method of the H5 avian influenza detection kit can comprise the following steps:

1. collecting a sample

Sample types include saliva, tissue, stool, etc.; the acquisition method comprises the following steps:

(1) sampling is carried out using a cotton swab or swab at a suitable location (e.g., in the trachea, intestines, cloaca, feces, etc. of the bird). For dead adult individuals, clear trachea or lesion parts are collected after dissection, and sputum samples do not need to be collected. When samples around the yolk pedicle of the duodenum and the middle section of the small intestine are collected, the opened intestinal surface is slightly scraped by scissors, and samples are taken at the exposed parts of the intestinal lymph follicular swelling, bleeding and ulcer. The detection effect on the young poultry is weakened, and the sampling can be performed by autopsy.

When sampling for many times, the sample is not too viscous, which is not favorable for the flow of the gold label on the test paper. The fecal sample is controlled to be below 0.2g, and the tissue or fluid sample is smeared with a cotton swab.

(2) The sampled cotton swab or the sampled swab is inserted into a sampling tube filled with an eluent (the eluent can be water) and stirred for 10 times, a tube cover is covered and shaken, so that the virus is released from the sample into the eluent as much as possible, and the sample is kept still for 1 min. And (4) taking a cotton swab or a sampling swab after sampling, and paying attention to safety protection. If the conjugate is found to flow poorly due to this reason during the dropping process, a drop of unused treatment fluid or eluent can be added to the opening of the test strip to assist the flow, and the other end of the sampling swab or cotton swab can be gently stirred at the dropping hole to assist the penetration of the sample fluid. When the samples are sampled in batches, the sampled samples can be put into a sample bag, sealed and marked by a marking pen.

2. Test sample

(1) The detection personnel need to wear epidemic prevention clothes, wear safety protection equipment such as a mask, medical surgical sterile gloves, goggles and the like, and strictly comply with the epidemic disease detection work flow.

(2) The clear liquid on the upper part of the sample after treatment is absorbed by a suction pipe 2 and is dripped to the sample adding window of the detection card, the dripping amount is about 80uL, the normal chromatography of the sample is ensured, and the liquid amount can be properly increased when the chromatography cannot be carried out.

(3) Observing the result displayed on the observation window for 15-20 min.

3. Result judgment

As shown in fig. 4, from left to right, the results were negative, positive, invalid and invalid, respectively. That is, the result of C-line color development and T-line no-color development was negative, the result of C-line color development and T-line color development was positive, and the result of C-line no-color development was ineffective.

A negative result indicates that the sample has no avian influenza antigen or the content of the avian influenza antigen is lower than the lowest threshold level of product detection; the positive result indicates that the sample contains the avian influenza antigen which is higher than the lowest threshold level detected by the kit; the result is invalid, which means that the detection result is invalid and needs to be detected again.

The specificity of the kit can reach 100%, and the test accuracy is more than or equal to 80%; the sensitivity is less than or equal to 50 pg/ml; the kit has obvious effect on adult poultry and can directly determine cloaca dung samples. And the operation is simple, no complex equipment is needed, the requirement on operators is not high, and the operation is easy.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the utility model, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims

1. An H5 avian influenza detection kit, which is characterized by comprising: the device comprises a sampling tool (1), a suction pipe (2) and a detection card (3); the sampling tool (1) comprises a sampling swab or a cotton swab; the sampling tool (1) also comprises a sampling pipe, wherein eluent is filled in the sampling pipe; the detection card (3) comprises a shell (31) and detection test paper, and the detection test paper is positioned in the shell (31); the detection test paper comprises a sample pad (34), a colloidal gold combination pad (35), a chromatographic membrane (36) and a water absorption pad (37), wherein one end of the sample pad (34) is connected with one end of the colloidal gold combination pad (35), the other end of the colloidal gold combination pad (35) is connected with one end of the chromatographic membrane (36), and the other end of the chromatographic membrane (36) is connected with one end of the water absorption pad (37); a test line (32) and a quality control line (33) are arranged at the position of the colloidal gold bonding pad (35); the shell (31) is provided with a sample adding window and an observation window, the sample adding window is used for adding a sample to be detected, and the observation window is used for observing the change conditions of the test line (32) and the quality control line (33) before and after detection.

2. The avian influenza H5 detection kit according to claim 1, wherein a sample can be loaded onto the sample pad (34) through the loading window of the housing (31), and the change of the test line (32) and the quality control line (33) before and after detection can be observed through the observation window; the material of the test line (32) and the material of the quality control line (33) are both nitrocellulose membrane strips.

3. The avian influenza test kit of H5 according to claim 1, wherein the pipette (2) is hollow inside, and the pipette (2) comprises a first tubular member (21), a second tubular member (22), a third tubular member (23) and a fourth tubular member (24); one end of the first tubular member (21) is communicated with one end of the second tubular member (22), the other end of the second tubular member (22) is communicated with one end of the third tubular member (23), and the other end of the third tubular member (23) is communicated with one end of the fourth tubular member (24); the other end of the first tubular part (21) is closed, and the other end of the fourth tubular part (24) is open and is used for sucking a sample to be detected.

4. The avian influenza test kit of claim 3, wherein the tube diameter of the first tubular member (21) is smaller than the tube diameter of the second tubular member (22), the tube diameter of the second tubular member (22) is larger than the tube diameter of the third tubular member (23), and the tube diameters of the first tubular member (21), the second tubular member (22) and the third tubular member (23) are all larger than the tube diameter of the fourth tubular member (24).

5. The H5 avian influenza detection kit according to claim 3, wherein the first tubular member (21), the second tubular member (22), the third tubular member (23), and the fourth tubular member (24) are integrally formed to constitute the pipette (2).

6. The avian influenza H5 detection kit according to any one of claims 1 to 5, wherein the colloidal gold conjugate pad (35) comprises colloidal gold conjugated with an anti-avian influenza antibody; the test line (32) is pre-coated with an H5 type specific avian influenza antibody, and the quality control line (33) is pre-coated with a quality control line antibody.

7. The avian influenza H5 detection kit according to any one of claims 1 to 5, wherein the sampling tool (1) further comprises sterile gloves, a mask, goggles, scissors, a marker pen, a sample bag, and a timer.

8. The H5 avian influenza detection kit according to any one of claims 1 to 5, wherein the area of the observation window is larger than the area of the sample addition window; the distance between the sample adding window and the test line (32) is smaller than the distance between the sample adding window and the quality control line (33).

9. The H5 avian influenza detection kit according to any one of claims 1 to 5, wherein the straw (2) is made of plastic; the straw (2) is also provided with scale marks.

10. The avian influenza H5 detection kit according to any one of claims 1 to 5, wherein the sampling tube comprises a tube cap and a tube body, the tube cap is detachably disposed at the tube port of the tube body, and the tube body contains the eluent.