CN2162467Y - 细胞电穿孔融合仪 - Google Patents

细胞电穿孔融合仪 Download PDF

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CN2162467Y
CN2162467Y CN 93224890 CN93224890U CN2162467Y CN 2162467 Y CN2162467 Y CN 2162467Y CN 93224890 CN93224890 CN 93224890 CN 93224890 U CN93224890 U CN 93224890U CN 2162467 Y CN2162467 Y CN 2162467Y
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phase reversal
cell
reversal switch
fusion instrument
switch
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邹翔
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Zou Xiang
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion

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Abstract

一种脉冲输出可正、可负、也可正负交替出现的 细胞电穿孔融合仪，它是在已有的细胞电穿孔融合仪 的输出端加装一个倒相开关，这样就可以方便地实现 脉冲极性变化输出。

Description

本实用新型涉及一种可实现细胞外源基因转移和细胞电融合的装置。该装置尤其能提供正负交替的脉冲，提高基因转移和细胞融合的效率。
目前，公知的细胞电穿孔融合仪是由正弦波发生器和脉冲发生器（矩型脉冲或RC尖脉冲）组成。细胞电融合的实现过程为，在正弦交变电场的作用下，细胞可经电介质电泳形成珠串排列，造成细胞间的紧密接触，然后施加数个脉冲诱导相邻细胞的细胞膜在接触区穿孔而融合。细胞外源基因转移的实现过程为，细胞在脉冲电场的作用下，细胞膜可逆电穿孔，外源基因可以进入细胞内部。但是，目前已商品化的一般电融合仪的输出脉冲极性固定，在实验中连续输出的几个脉冲都是同方向的，由于细胞在电穿孔时有不对称击穿现象存在，同方向脉冲影响了基因转移和细胞融合效率的充分发挥。
本实用新型的目的是提供一种细胞电穿孔融合仪，它的脉冲输出极性可正、可负，也可正负交替。正负交替的脉冲可以克服细胞的不对称击穿现象，提高细胞融合率和外源基因转移效率。
本实用新型的目的是这样实现的：该仪器由正弦波发生器和矩型脉冲发生器组成，在仪器的输出端加装一个倒相开关由它来控制脉冲的输出极性。该倒相开关采用双刀双投机械开关，它的两个刀位接输出插孔，两组各两个触点交叉连接后，相对应的两个触点接原输出线路，另两个对应触点悬空。
由于采用上述方案，可以方便地控制脉冲的输出极性，且结构简单。
下面结合附图对本实用新型进一步说明。
图1是本实用新型的框图。
图2是本实用新型的电原理图。
图中1.正弦波发生装置        2.脉冲波发生装置        3.脉冲／正弦转换开关（兼脉冲触发开关）        4.倒相开关        5.输出插孔        6.集成线路块
在图1和图2中，在仪器的输出端装有倒相开关（4），它采用一个双刀双投机械开关，可以控制脉冲波的输出极性。该开关的触点a与c’连接，a’与c连接，刀位b和b’接输出插孔，触点a接3，a’接地。假设拨向左边为输出正脉冲，则拨向右边就可输出负脉冲，连续输出脉冲时若左右交替拨动该开关，则可输出正负交替脉冲。

Claims (4)

1、一种细胞电穿孔融合仪，它是由正弦波发生装置和脉冲波发生装置组成，其特征是：在输出端之前电连接倒相开关，该倒相开关采用双刀双投机械开关，它的两个刀位接输出插孔，两组各两个触点交叉连接后相对应的两个触点接原输出线路，另两个对应触点悬空。
2、根据权利要求1所述的细胞电穿孔融合仪，其特征是所述倒相开关为机械倒相开关。
3、根据权利要求1所述的细胞电穿孔融合仪，其特征是所述倒相开关为电子倒相开关。
4、根据权利要求1所述的细胞电穿孔融合仪，其特征是所述倒相开关为继电器倒相开关。
CN 93224890 1993-09-24 1993-09-24 细胞电穿孔融合仪 Expired - Fee Related CN2162467Y (zh)

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CN 93224890 CN2162467Y (zh) 1993-09-24 1993-09-24 细胞电穿孔融合仪

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CN 93224890 CN2162467Y (zh) 1993-09-24 1993-09-24 细胞电穿孔融合仪

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102124102A (zh) * 2008-07-18 2011-07-13 麦克赛特股份有限公司 优化电穿孔的方法

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102124102A (zh) * 2008-07-18 2011-07-13 麦克赛特股份有限公司 优化电穿孔的方法
CN102124102B (zh) * 2008-07-18 2013-12-18 麦克赛特股份有限公司 优化电穿孔的方法

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Free format text: CORRECT: PATENTEE ADDRESS; FROM: 100052 NO.100, YINGXIN STREET, XUANWU DISTRICT, BEIJING CITY TO: ROOM #, WEISHENGWUJIAOYAN, YIXUE YARD, NANJING COLLEGE, NO. 22, HANKOU ROAD, GULOU DISTRICT, NANJING CITY, 210008

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