CN216233698U - Kit for transforming CIK cells through micro-ring DNA-EGFR gene - Google Patents

Abstract

The utility model discloses a kit for transforming CIK cells by using a micro-ring DNA-EGFR gene, which comprises: shell, mount and place the hole, the mount install in the inner chamber top of shell, it is a plurality of to place hole quantity, is the array respectively and offers in the outer wall of mount still includes: the clamping mechanism is assembled in the inner cavity of the fixed frame; the pushing mechanism is assembled in the inner cavity of the shell; and the pressing rod is assembled at the bottom end of the inner cavity of the shell. This micro-ring DNA-EGFR gene transformation CIK cell kit, in the in-service use, can press from both sides tightly the test tube of placing in this equipment, and the test tube that not only is applicable to different thicknesses is fixed, and the test tube damages because of the collision when can avoiding the mobile device in addition, can also release corresponding test tube alone when needs use the medicament, and is quick, convenient as far as not, can prevent moreover that the mistake from bumping or taking other medicaments by the mistake.

Description

Kit for transforming CIK cells through micro-ring DNA-EGFR gene

Technical Field

The utility model relates to the technical field of cell culture equipment, in particular to a kit for transforming CIK cells by using a micro-ring DNA-EGFR gene.

Background

The killer cell induced by the cell factor is called CIK cell for short, is used for cell adoptive immunotherapy, and is a biotherapeutic method for returning the immune cell induced in vitro and having strong tumor killing activity to a patient, which not only has the capability of killing residual cancer cells in vivo, but also can enhance the immunity of a host, and becomes one of important means for tumor biotherapy;

EGFR is a receptor for epithelial growth factor cell proliferation and signal conduction, DNA-EGFR gene transformation CIK cells refer to eukaryotic expression vectors and micro-ring DNA vectors formed by fusing EGFR antibody genes and tumor specific receptor CAR genes, and when the CIK cells are deeply researched, induced and cultured, kits provided with various standard reagents are required to be prepared so as to facilitate culture research;

it is inside mostly the integral type design that the kit that uses in the present stage, it is comparatively inconvenient to take and deposit reagent, can touch the part outside the test tube in the kit usually when taking moreover, cause the bacterium to contact, in addition, current equipment can't advance an item adjustment according to the model of test tube, owing to can't adjust the internal diameter of storage hole, if thinner test tube is placed in the storage hole, can take place to rock, the test tube strikes the box body easily when removing to lead to the test tube damage of being equipped with reagent.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a kit for transforming CIK cells by using a micro-ring DNA-EGFR gene, which aims to solve the problems that the storage and taking ratio of test tubes is inconvenient and the inner diameter of a storage hole cannot be adjusted according to the size of the test tube in the background technology.

In order to achieve the purpose, the utility model provides the following technical scheme: a kit for transforming CIK cells by using micro-ring DNA-EGFR genes comprises: shell, mount and place the hole, the mount install in the inner chamber top of shell, it is a plurality of to place hole quantity, is the array respectively and offers in the outer wall of mount still includes:

the clamping mechanism is assembled in the inner cavity of the fixed frame;

the pushing mechanism is assembled in the inner cavity of the shell;

the pressing rod is assembled at the bottom end of the inner cavity of the shell;

the fourth spring is sleeved on the outer wall of the pressing rod, and two ends of the fourth spring are respectively and fixedly connected to the inner wall of the shell and the outer wall of the pressing rod;

the clamping mechanism includes:

the first springs are two in number, and one ends of the first springs are symmetrically and fixedly arranged on the left side and the right side of the inner cavity of the fixing frame respectively;

one end of the clamping block is fixedly arranged at the other end of the first spring, and the other end of the clamping block extends into the inner cavity of the placing hole;

the four sliding chutes are respectively arranged on the left side and the right side of the upper end and the lower end of the inner cavity of the fixing frame;

the slider, slidable embedded in the inner chamber of spout, and inboard fixed mounting in the outer wall of clamp splice extrudees the clamp splice to both sides and can make the clamp splice remove to the left and right sides respectively, makes first spring compelled to compress simultaneously, after removing external force, utilizes the elasticity of first spring to make the clamp splice reset.

Preferably, the part of the clamping block extending into the inner cavity of the placing hole is arranged in an arc shape.

Preferably, the pushing mechanism includes: the two frames are respectively and symmetrically arranged in the inner cavity of the shell; the number of the through holes is three, and the through holes are respectively arranged on the outer wall of the frame at equal intervals; the first fixing rod is fixedly arranged on the left side of the top end of the inner side of the frame; the limiting assembly is assembled in the inner cavity of the frame; the clamping components are three in number and are assembled in the inner cavity of the frame at equal intervals respectively.

Preferably, the limiting assembly comprises: the moving plate is slidably embedded in the inner cavity of the frame; the slideway is arranged on the outer wall of the moving plate, and the first fixing rod penetrates through the inner cavity of the slideway; the second fixed rod is arranged on the front side ordering tube of the moving plate; the reset groove is formed at the bottom end of the outer wall of the moving plate; the two limiting grooves are formed in the right side of the bottom end of the moving plate; and the two ends of the second spring are respectively fixedly connected with the outer walls of the first fixed rod and the second fixed rod, the second spring is forced to stretch when the movable plate slides, and the movable plate is driven to reset under the action of the self elasticity of the second spring after the external force is removed.

Preferably, the click assembly includes: the two ends of the push rod respectively penetrate through the upper end and the lower end of the frame; the inclined plane is arranged at the bottom end of the push rod; one end of the limiting rod is fixedly arranged on the outer wall of the push rod, and the other end of the limiting rod penetrates through and extends out of the inner cavity of the through hole; the third spring is sleeved on the outer wall of the push rod, and two ends of the third spring are respectively and fixedly connected to the outer walls of the frame and the push rod; and the push plate is fixedly arranged at the top end of the push rod, the third spring can be contracted by upwards pushing the push rod, the movable plate slides, and the push rod is reset by removing external force and utilizing the elasticity of the third spring.

Preferably, the circle center of the push plate is coaxial with the center of the inner cavity of the placing hole.

Preferably, the width of the top end of the lug in the inner cavity of the limiting groove is larger than the diameter of the limiting rod.

Compared with the prior art, the utility model has the beneficial effects that: this micro-ring DNA-EGFR gene conversion CIK cell kit, through placing the test tube in the inner chamber of placing the hole, promote two clamp splices respectively to both sides, utilize the elasticity of first spring to press from both sides the test tube simultaneously tightly, make push rod rebound through inwards pressing down the press bar, utilize gag lever post and spacing groove looks joint, can brake the push rod, will place upwards releasing at the test tube of placing the hole inboard, in-service use, can press from both sides tightly the test tube of placing in this equipment, not only be applicable to the test tube of different thicknesses fixed, and the test tube damages because of the collision when can avoiding the mobile device, can also use the medicament when needs, release corresponding test tube alone, not as fast as possible, it is convenient, and can prevent that the mistake from bumping or taking other medicaments by mistake.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a top cross-sectional view of the clamping mechanism of the present invention;

FIG. 3 is a schematic view of the pushing mechanism of the present invention;

FIG. 4 is a schematic view of the structure of the moving plate of the present invention;

FIG. 5 is a right side sectional view of the pressing rod according to the present invention.

In the figure: 1. the device comprises a shell, 2, a fixing frame, 3, a placing hole, 4, a clamping mechanism, 41, a first spring, 42, a clamping block, 43, a sliding groove, 44, a sliding block, 5, a pushing mechanism, 51, a frame, 52, a through hole, 53, a first fixing rod, 54, a moving plate, 55, a sliding way, 56, a second fixing rod, 57, a resetting groove, 58, a limiting groove, 59, a second spring, 510, a push rod, 511, an inclined plane, 512, a limiting rod, 513, a third spring, 514, a push plate, 6, a pressing rod, 7 and a fourth spring.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-5, the present invention provides a technical solution: a micro-ring DNA-EGFR gene conversion CIK cell kit comprises a shell 1, a fixing frame 2 and placing holes 3, wherein the fixing frame 2 is arranged at the top end of an inner cavity of the shell 1, the placing holes 3 are a plurality of and are respectively arranged on the outer wall of the fixing frame 2 in an array manner, the kit also comprises a clamping mechanism 4, a pushing mechanism 5, a pressing rod 6 and a fourth spring 7, the clamping mechanism 4 is assembled in the inner cavity of the fixing frame 2, the pushing mechanism 5 is assembled in the inner cavity of the shell 1, the pressing rod 6 is assembled at the bottom end of the inner cavity of the shell 1, the fourth spring 7 is sleeved on the outer wall of the pressing rod 6, two ends of the fourth spring are respectively and fixedly connected to the inner wall of the shell 1 and the outer wall of the pressing rod 6, the fourth spring 7 is a spiral spring and is stretched or extruded to generate elastic deformation, the initial state of external force is recovered, when the pressing rod 6 is stressed to move inwards, the fourth spring 7 is forced to be compressed and after the external force is removed, the pressing rod 6 is driven to reset under the action of the self elasticity of the fourth spring 7;

the clamping mechanism 4 includes two first springs 41, clamp blocks 42, sliding chutes 43, and sliding blocks 44, where one end of each of the two first springs 41 is symmetrically and fixedly installed at the left and right sides of the inner cavity of the fixing frame 2, the first spring 41 is a spiral spring, and is elastically deformed by stretching or squeezing, and the external force is removed to restore the initial state, the clamp blocks 42 are squeezed to the two sides to enable the clamp blocks 42 to move to the left and right sides, respectively, and simultaneously enable the first spring 41 to be forcibly compressed, and after the external force is removed, the clamp blocks 42 can be restored by the elastic force of the first spring 41, one end of each of the clamp blocks 42 is fixedly installed at the other end of the first spring 41, and the other end of each of the clamp blocks extends into the inner cavity of the placing hole 3, the four sliding chutes 43 are respectively installed at the upper and lower ends and left sides of the inner cavity of the fixing frame 2, and the sliding blocks 44 are slidably embedded in the inner cavity of the sliding chutes 43, and the inner sides of the clamping blocks are fixedly installed on the outer wall of the clamp blocks 42.

Preferably, the part of the clamping block 42 extending into the inner cavity of the placing hole 3 is arranged in an arc shape, so that the contact area between the clamping block and the outer wall of the test tube is increased, and the stress is dispersed.

Preferably, the pushing mechanism 5 includes a frame 51, a through hole 52, a first fixing rod 53, a moving plate 54, a slide 55, a second fixing rod 56, a reset groove 57, a limiting groove 58, a second spring 59, a push rod 510, an inclined plane 511, a limiting rod 512, a third spring 513, and a push plate 514, wherein the number of the frame 51 is two, the two frames are respectively and symmetrically installed in the inner cavity of the housing 1, the number of the through holes 52 is three, the three through holes are respectively and equidistantly installed on the outer wall of the frame 51, the first fixing rod 53 is fixedly installed on the left side of the top end of the inner side of the frame 51, the moving plate 54 is slidably embedded in the inner cavity of the frame 51, the slide 55 is installed on the outer wall of the moving plate 54, the first fixing rod 53 penetrates through the inner cavity of the slide 55, the second fixing rod 56 is installed on the front order tube of the moving plate 54, the reset groove 57 is installed on the bottom end of the outer wall of the moving plate 54, the two limiting grooves 58 are installed on the right side of the bottom end of the moving plate 54, the two ends of the second spring 59 are respectively and fixedly connected to the outer walls of the first fixing rod 53 and the second fixing rod 56, the second spring 59 is a coil spring, and is stretched or extruded to generate elastic deformation, the external force is removed to restore the initial state, the second spring 59 is forced to stretch when the moving plate 54 slides, after the external force is removed, the moving plate 54 is driven to restore under the action of the self elastic force of the second spring 59, the two ends of the push rod 510 respectively penetrate through the upper end and the lower end of the frame 51, the inclined plane 511 is arranged at the bottom end of the push rod 510, the push rod 510 can be conveniently pushed by the pressing rod 6 to move upwards by using the inclined plane 511, one end of the limiting rod 512 is fixedly arranged on the outer wall of the push rod 510, the other end of the limiting rod penetrates through and extends out of the inner cavity of the through hole 52, the third spring 513 is sleeved on the outer wall of the push rod 510, and the two ends are respectively and fixedly connected to the outer walls of the frame 51 and the push rod 510, the third spring 513 is a coil spring, and is stretched or extruded to generate elastic deformation, the initial state is restored by removing the external force, the push plate 514 is fixedly installed at the top end of the push rod 510, the third spring 513 is contracted by pushing the push rod 510 upwards, the moving plate 54 is slid, and the push rod 510 is restored by removing the external force by using the elastic force of the third spring 513.

As a preferred scheme, furthermore, the center of the push plate 514 is coaxial with the center of the inner cavity of the placing hole 3, so that the bottom end of the test tube is accurately placed in the center of the push plate 514.

Preferably, the width of the top end of the projection in the inner cavity of the limiting groove 58 is larger than the diameter of the limiting rod 512, so that the limiting rod 512 and the limiting groove 58 are stably clamped and prevented from sliding.

The detailed connection means is well known in the art, and the following mainly describes the working principle and process, and does not describe the electrical control.

The method comprises the following steps: the test tube is inserted into the inner cavity of the placing hole 3 from top to bottom, under the limiting action of the outer wall of the test tube, the two clamping blocks 42 respectively move towards two sides and extrude the first spring 41, and meanwhile, the clamping blocks 42 are forced to have the trend of moving reversely by the aid of the elasticity of the body of the first spring 41, so that the test tube is clamped;

step two: when the medicine needs to be taken, the pressing rod 6 corresponding to the test tube filled with the corresponding medicine is pressed, the push rod 510 moves upwards under the limiting action of the pressing rod 6, the moving plate 54 moves rightwards and stretches the second spring 59 under the limiting action of the limiting rod 512 on the lug on the inner side of the limiting groove 58, after the limiting rod 512 is positioned at the top end of the lug on the inner side of the limiting groove 58, the limiting rod 512 is clamped with the limiting groove 58 under the action of the elastic force of the second spring 59 to limit the push rod 510, the third spring 513 is forced to compress at the moment, the push plate 514 pushes out the corresponding test tube at the same time, when other medicine needs to be taken, the other pressing rod 6 is pressed to push the other push rod 510 to move upwards, under the limiting action of the lug on the limiting groove 58, the moving plate 54 moves rightwards, the push rod 510 which is limited before is separated from the limited state and resets under the action of the elastic force of the third spring 513, when the other limiting rod 512 is clamped with the limiting groove 58 in the opposite direction, the other reagent can be taken;

step three: when the use is finished, the leftmost pressing rod 6 is pressed and the corresponding push rod 510 is pushed upwards, the moving plate 54 moves rightwards under the limiting action of the inner wall of the resetting groove 57, any limiting rod 512 limited is reset, after the external force is removed, the push rods 510 are reset under the elastic force action of the third spring 513, and all the push rods 510 are reset at the moment;

in the in-service use, can release arbitrary test tube alone, guarantee that the medicament takes accuracy, convenience, quick when using, and can not touch other test tubes, can also press from both sides the test tube of tight different thicknesses to prevent that the transport test tube from bumping on the way, avoid the test tube to damage.

In the description of the present invention, it is to be understood that the terms "coaxial", "bottom", "one end", "top", "center position", "the other end", "up", "one side", "top", "inside", "front", "center", "both ends", and the like, indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation; also, unless expressly stated or limited otherwise, the terms "snap-fit," "plug-in," "weld," "mount," "set," "interference fit," "screw-on," "pin-on," and the like are to be construed broadly, e.g., as a fixed connection, a removable connection, or an integral whole; can be mechanically or electrically connected; the terms may be directly connected or indirectly connected through an intermediate, and may be communication between two elements or interaction relationship between two elements, unless otherwise specifically limited, and the specific meaning of the terms in the present invention will be understood by those skilled in the art according to specific situations.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims (7)

1. A kit for transforming CIK cells by using micro-ring DNA-EGFR genes comprises: shell (1), mount (2) and place hole (3), mount (2) install in the inner chamber top of shell (1), place hole (3) quantity and be a plurality of, be the array respectively and set up in the outer wall of mount (2), its characterized in that still includes:

the clamping mechanism (4) is assembled in the inner cavity of the fixed frame (2);

the pushing mechanism (5) is assembled in the inner cavity of the shell (1);

the pressing rod (6) is assembled at the bottom end of the inner cavity of the shell (1);

the fourth spring (7) is sleeved on the outer wall of the pressing rod (6), and two ends of the fourth spring are respectively and fixedly connected to the inner wall of the shell (1) and the outer wall of the pressing rod (6);

the clamping mechanism (4) comprises:

the number of the first springs (41) is two, and one ends of the first springs are symmetrically and fixedly arranged on the left side and the right side of the inner cavity of the fixing frame (2) respectively;

the clamping block (42) is fixedly arranged at one end of the first spring (41) and extends into the inner cavity of the placing hole (3) at the other end;

the four sliding chutes (43) are respectively arranged on the left side and the right side of the upper end and the lower end of the inner cavity of the fixed frame (2);

the sliding block (44) is slidably embedded in the inner cavity of the sliding groove (43), the inner side of the sliding block is fixedly installed on the outer wall of the clamping block (42), the clamping block (42) can be extruded towards the two sides to enable the clamping block (42) to move towards the left side and the right side respectively, the first spring (41) is forced to be compressed, and after the external force is removed, the clamping block (42) can be reset by the aid of elastic force of the first spring (41).

2. The kit for transforming CIK cells by using micro-ring DNA-EGFR gene according to claim 1, wherein: the part of the clamping block (42) extending into the inner cavity of the placing hole (3) is arranged in an arc shape.

3. The kit for transforming CIK cells by using micro-ring DNA-EGFR gene according to claim 1, wherein: the pushing mechanism (5) comprises:

two frames (51) which are respectively and symmetrically arranged in the inner cavity of the shell (1);

the number of the through holes (52) is three, and the through holes are respectively arranged on the outer wall of the frame (51) at equal intervals;

a first fixing rod (53) fixedly installed on the left side of the top end of the inner side of the frame (51);

the limiting assembly is assembled in the inner cavity of the frame (51);

the clamping components are three in number and are respectively assembled in the inner cavity of the frame (51) at equal intervals.

4. The kit for transforming CIK cells by using micro-ring DNA-EGFR gene according to claim 3, wherein: the spacing subassembly includes:

a moving plate (54) slidably fitted in the inner cavity of the frame (51);

the slideway (55) is arranged on the outer wall of the moving plate (54), and the first fixing rod (53) penetrates through the inner cavity of the slideway (55);

a second fixing rod (56) mounted on the front side ordering tube of the moving plate (54);

the reset groove (57) is formed in the bottom end of the outer wall of the moving plate (54);

the limiting grooves (58) are two in number and are formed in the right side of the bottom end of the moving plate (54);

and the two ends of the second spring (59) are respectively and fixedly connected with the outer walls of the first fixing rod (53) and the second fixing rod (56), the second spring (59) is forced to stretch when the moving plate (54) slides, and the moving plate (54) is driven to reset under the action of the self elasticity of the second spring (59) after the external force is removed.

5. The kit for transforming CIK cells by using micro-ring DNA-EGFR gene according to claim 3, wherein: the chucking assembly includes:

a push rod (510) having both ends respectively penetrating the upper and lower ends of the frame (51);

a slope (511) provided at a bottom end of the push rod (510);

one end of the limiting rod (512) is fixedly arranged on the outer wall of the push rod (510), and the other end of the limiting rod penetrates through and extends out of the inner cavity of the through hole (52);

the third spring (513) is sleeved on the outer wall of the push rod (510), and two ends of the third spring are respectively and fixedly connected to the outer walls of the frame (51) and the push rod (510);

and the push plate (514) is fixedly arranged at the top end of the push rod (510), the third spring (513) can be contracted by pushing the push rod (510) upwards, the moving plate (54) can slide, and the push rod (510) is reset by removing external force and utilizing the elastic force of the third spring (513).

6. The kit for transforming CIK cells by using micro-ring DNA-EGFR gene according to claim 5, wherein: the circle center of the push plate (514) is coaxial with the center of the inner cavity of the placing hole (3).

7. The kit for transforming CIK cells by using micro-ring DNA-EGFR gene according to claim 4, wherein: the width of the top end of the lug in the inner cavity of the limiting groove (58) is larger than the diameter of the limiting rod (512).

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