CN216224417U - Mixing assembly and synthesizer equipment with same - Google Patents

Abstract

The utility model provides a mixing assembly and synthesizer equipment with the same. The mixing assembly comprises: an upper reaction plate; a lower reaction plate superposed with the upper reaction plate; an inlet flow channel and an outlet flow channel which are arranged between the upper reaction plate and the lower reaction plate; the reaction unit is arranged between the upper reaction plate and the lower reaction plate and comprises a first mixed buffer area, a mixed reaction flow channel and a second mixed buffer area which are sequentially connected, the first mixed buffer area is communicated with the second mixed buffer area through the mixed reaction flow channel, the first mixed buffer area is communicated with the inlet flow channel, and the second mixed buffer area is communicated with the outlet flow channel; and the static mixing structure is arranged in the mixing reaction flow channel to increase the speed gradient of the laminar flow movement of the fluid or form a vortex so as to mix the materials to be mixed entering the mixing reaction flow channel. The technical scheme of the utility model can solve the problem of low reaction efficiency of the synthesizer in the prior art.

Description

Mixing assembly and synthesizer equipment with same

Technical Field

The utility model relates to the technical field of synthesis reaction, in particular to a mixing assembly and synthesizer equipment with the same.

Background

In a continuous reactor used in production, two or more fluids are generally injected into a mixing component of the continuous reactor, the fluids are in static natural mixing in a mixing space, uneven mixing is easy to occur in the flowing process, the fluid distribution uniformity is low, and the fluid flow rate in a channel form is also low, so that the mass and heat transfer efficiency is greatly limited, and the mass and heat transfer efficiency is low. Meanwhile, the pressure loss of the fluid is too large, so that the inlet pressure of the whole reactor is too large, and the reaction yield of the whole reactor is low.

SUMMERY OF THE UTILITY MODEL

The utility model mainly aims to provide a mixing assembly and synthesizer equipment with the same, and aims to solve the problem of low reaction efficiency caused by uneven mixing of fluids in the mixing assembly in the related art.

To achieve the above object, according to one aspect of the present invention, there is provided a mixing assembly including: an upper reaction plate; a lower reaction plate superposed with the upper reaction plate; an inlet flow channel and an outlet flow channel which are arranged between the upper reaction plate and the lower reaction plate; the reaction unit is arranged between the upper reaction plate and the lower reaction plate and comprises a first mixed buffer area, a mixed reaction flow channel and a second mixed buffer area which are sequentially connected, the first mixed buffer area is communicated with the second mixed buffer area through the mixed reaction flow channel, the first mixed buffer area is communicated with the inlet flow channel, and the second mixed buffer area is communicated with the outlet flow channel; and the static mixing structure is arranged in the mixing reaction flow channel to increase the speed gradient of the laminar flow movement of the fluid or form a vortex so as to mix the materials to be mixed entering the mixing reaction flow channel.

Further, the static mixing structure comprises a plurality of spiral sections, the plurality of spiral sections comprise a first spiral section and a second spiral section, at least one of the first spiral section and the second spiral section is formed by twisting a rectangular plate by 180 degrees, and the first spiral section and the second spiral section are alternately arranged.

Furthermore, the first spiral section and the second spiral section are both made by twisting a rectangular plate by 180 degrees, the first spiral section and the second spiral section respectively comprise a dividing edge positioned at the front end and a drainage edge positioned at the rear end, and an included angle is formed between the dividing edge of the first spiral section and the drainage edge of the second spiral section.

Further, static hybrid still includes the center pin and sets up a plurality of blades on the center pin, and a plurality of blades set up along the axis direction interval of center pin, and the blade is the contained angle setting with the center pin.

Further, the mixing reaction flow channel comprises a first flow channel groove arranged on the upper reaction plate and a second flow channel groove arranged on the lower reaction plate, the first flow channel groove and the second flow channel groove are arranged in a crossed mode, and a static mixing structure is arranged in at least one of the first flow channel groove and the second flow channel groove.

According to another aspect of the present invention, there is provided a synthesizer apparatus comprising a feeding unit and a mixing assembly connected to the feeding unit, the mixing assembly being as described above.

Further, the feed unit includes a plurality of first feeding valves, correspond a plurality of second feeding valves and a plurality of control valve of being connected with a plurality of first feeding valves, each control valve setting is between first feeding valve and the second feeding valve that corresponds, the feed unit still includes the pipeline that is used for connecting a plurality of control valves, so that the material that gets into through first feeding valve and second feeding valve passes through pipeline output, the feed unit still includes supplementary washing pipeline, supplementary washing pipeline is connected with a control valve that lies in the outside among a plurality of control valve.

Further, at least one of the first and second inlet valve blocks comprises: the first feeding pipeline is connected with the corresponding second feeding valve group through a control valve; the feeding valve is arranged on the first feeding pipeline; the synthesizer equipment also comprises a main cleaning pipeline connected with the first feeding pipeline and a cleaning on-off valve arranged on the main cleaning pipeline; or the synthesizer equipment also comprises an on-off valve arranged on the auxiliary cleaning pipeline so as to control the on-off of the auxiliary cleaning pipeline; or the synthesizer equipment also comprises a control system, and the first feeding valve group, the second feeding valve group and the control valve are all connected with the control system.

Furthermore, the synthesizer equipment also comprises a synthesis column assembly, the synthesis column assembly comprises a synthesis column and a back washing device connected with the synthesis column, the synthesis column is provided with a reaction cavity, a first inlet and a first outlet which are communicated with the reaction cavity, the back washing device is used for back washing the reaction cavity from the first outlet to the first inlet.

Furthermore, the back washing device comprises a first washing pipeline communicated with the first outlet and a second washing pipeline connected with the first inlet, and the synthesizer equipment further comprises a power pump arranged on the first washing pipeline or the second washing pipeline, so that washing liquid enters the reaction cavity from the first outlet and flows out from the first inlet.

By applying the technical scheme of the utility model, the materials to be mixed can flow into the mixing component through the inlet flow channel, sequentially pass through the first mixing buffer area, the mixing reaction flow channel and the second mixing buffer area, and then flow out of the mixing component through the outlet flow channel. When the materials to be mixed enter the mixing reaction flow channel, the materials to be mixed and the static mixing structure collide for multiple times, so that the flow direction of the materials can be changed, and the fluid is more uniform in mixing, thereby improving the mixing effect and the mixing efficiency of the materials. The material flow in the mixed reaction runner is more disordered, so that the problems of uneven material mixing or uneven flow rate and instability can be solved, higher mixing uniformity is achieved, and the mixing efficiency of the materials can be improved. In addition, because the fluid is mixed more evenly under the action of the static mixing structure, the length size of the mixing reaction flow channel can be reduced, thereby reducing the volume of the whole mixing component and realizing the further optimization of space and structure.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of this application, illustrate embodiments of the utility model and, together with the description, serve to explain the utility model and not to limit the utility model. In the drawings:

FIG. 1 shows a schematic structural diagram of a synthesizer apparatus according to an embodiment of the utility model;

FIG. 2 shows a schematic structural diagram of a hybrid assembly of an embodiment of the present invention;

FIG. 3 illustrates a partial perspective view of the mixing assembly of FIG. 2;

FIG. 4 shows a schematic structural view of a static mixing structure of the mixing assembly of FIG. 3;

FIG. 5 shows a schematic structural view of a feed unit of the synthesizer apparatus of FIG. 1;

FIG. 6 shows a schematic structural diagram of a first embodiment of a synthesis column assembly of the synthesizer apparatus of FIG. 1 (wherein the direction of the arrows indicate the direction of flow of the material); and

fig. 7 shows a schematic structural diagram of a second embodiment of a synthesis column assembly of the synthesizer apparatus of fig. 1 (wherein the arrow direction indicates the flow direction of the material).

Wherein the figures include the following reference numerals:

1. a mixing assembly; 2. a feed unit; 10. an upper reaction plate; 20. a lower reaction plate; 21. a first feed line; 23. a first feed valve block; 24. a second valve block; 25. a delivery conduit; 27. A main cleaning pipeline; 30. a reaction unit; 31. entering a flow channel; 32. a discharge flow passage; 33. a first mixing buffer; 34. a mixing reaction flow channel; 341. a first flow channel groove; 342. a second flow channel groove; 35. a second mixing buffer; 40. a static mixing structure; 41. a first helical section; 42. a second helical segment; 43. dividing edges; 44. drainage edge; 60. synthesizing a column assembly; 61. synthesizing a column; 62. a backwashing device; 63. a reaction chamber; 64. a first inlet; 65. a first outlet; 66. a first flushing line; 67. a second flushing line; 80. auxiliary cleaning of the pipeline; 90. a control valve; 101. a support; 102. a partition plate; 103. a control system; 104. a flow control module; 105. a PAT monitoring module; 106. a pressure sensing module; 107. a backpressure regulation module.

Detailed Description

It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present invention will be described in detail below with reference to the embodiments with reference to the attached drawings.

It should be noted that the material to be mixed in the embodiment of the present invention is preferably a monomer & activator, clamping a & clamping B, used for synthesizing oligonucleotides.

Example one

As shown in fig. 1, the embodiment of the present invention provides certain improvements in the structure and layout of the various components in the synthesizer apparatus. The synthesizer equipment comprises a support 101 and a partition plate 102 arranged on the support, wherein the support 101 defines a cavity, and the cavity is divided into an upper accommodating cavity and a lower accommodating cavity by the partition plate 102; wherein the control system 103 is located in the upper receiving chamber and the feeding unit 2 is located in the lower receiving chamber. In addition, the flow control module 104, PAT monitoring module 105, pressure sensing module 106, and mixing assembly 1 form a modular structure and are located in the upper receiving chamber, and the above modular structure and control system 103 are horizontally arranged in the left and right directions.

Through the setting, each part can be reasonably arranged, so that the control system 103, the feeding unit 2, the flow control module 104, the PAT monitoring module 105, the pressure sensing module 106, the backpressure adjusting module 107, the mixing assembly 1 and the like are integrated on the support 101, the compact structure is ensured, the pipeline arrangement, the accommodating volume and the attractiveness can be considered, the effect of minimizing the dead volume of the fluid is realized, and the attractiveness and the practicability are both considered.

As shown in fig. 1, in the first embodiment of the present invention, the flow control module 104 may employ a high-precision flow meter. The flow control module 104 is connected to the control system and provides a real flow value/Process Value (PV) via an analog signal, which is compared to a user defined value (SP). Based on the difference between the measured PV and the user-defined SP, the control system continuously adjusts the signal sent to the pump motor to regulate the feed flow in real time. Typically, the flow control module 104 is mounted with the feed unit 2.

The PAT monitoring module 105 employs a high precision detector for monitoring. The detector may be a fixed or variable wavelength near infrared or ultraviolet detector, conductivity detector, temperature detector, humidity detector, pH detector, pressure transducer, etc. or any detector that can detect the characteristics of a critical (and/or variable) feed material and output a measurable signal, adjusted and supplemented as needed. Typically, the composition of the key component employs a variable wavelength ultraviolet detector. The detector feeds back monitoring signals to the controller for analysis of its relevant composition, temperature, humidity, pH and extent of reaction etc., the controller will compare PV to SP based on the deviation between the measured PV and the user defined SP, and if the measured deviation is outside the use "defined tolerance" an alarm is activated and liquid is not delivered to the reaction vessel to prevent synthesis of incorrect biomolecules or to affect product yield. Typically, this module is installed before the reactor feed.

Building block components may need to be activated by reagents in order for the building block to be chemically linked to the starting components or partially completed molecules in the mixing module or to require mixing in optimal proportions. In one embodiment of the present invention, the pressure sensing module 106 is a precision pressure sensor installed before the reactor is fed to adjust the liquid delivery to the reactor so that a uniform pressure can be maintained in the reaction vessel.

In some embodiments, the PAT monitoring module 105 is located downstream of the reactor module to detect the composition of the fluid passing through the reaction vessel and communicate it to the controller. The control strategy may advance subsequent steps based on the composition of the effluent from the reaction vessel, which may indicate the completeness of the reaction due to consumption of key components and the resulting change in the detector output signal. Such a strategy may include implementing and controlling a recirculation step by an optional recirculation module, which may continue until full utilization of the reactants. The detector may be a fixed or variable wavelength near infrared or ultraviolet detector, conductivity detector, temperature detector, humidity detector, pH detector, pressure transducer, etc. or any detector that can detect the characteristics of a critical (and/or variable) feed and output a measurable signal adjusted and supplemented as desired, typically a variable wavelength ultraviolet detector is used for the composition of the critical component.

In a first embodiment of the utility model, the synthesizer apparatus further comprises a backpressure regulation module 107 located downstream of the reactor module. Preferably, the backpressure regulation module 107 employs an automatic backpressure valve. By providing the backpressure regulating module 107, a stable and uniform backpressure of the reactor can be maintained.

In order to solve the problem of the prior art that the fluid mixing in the continuous reactor is not uniform, which results in low mass and heat transfer efficiency, the present invention provides a mixing assembly, as shown in fig. 2 and 3.

The mixing assembly 1 of the first embodiment includes an upper reaction plate 10, a lower reaction plate 20, an inlet flow channel 31, an outlet flow channel 32, a reaction unit 30 and a static mixing structure 40. Wherein the lower reaction plate 20 is superposed with the upper reaction plate 10; the inlet flow channel 31 and the outlet flow channel 32 are provided between the upper reaction plate 10 and the lower reaction plate 20; the reaction unit 30 is arranged between the upper reaction plate 10 and the lower reaction plate 20, the reaction unit 30 comprises a first mixing buffer zone 33, a mixing reaction flow channel 34 and a second mixing buffer zone 35 which are connected in sequence, the first mixing buffer zone 33 is communicated with the second mixing buffer zone 35 through the mixing reaction flow channel 34, the first mixing buffer zone 33 is communicated with the inlet flow channel 31, and the second mixing buffer zone 35 is communicated with the outlet flow channel 32; static mixing structures 40 are disposed within the mixing reaction channel 34 to increase the velocity gradient of the laminar fluid flow motion or to form vortices that mix the materials to be mixed entering the mixing reaction channel 34.

In the above technical solution, the material to be mixed can flow into the mixing component 1 through the inlet flow channel 31, and sequentially pass through the first mixing buffer area 33, the mixing reaction flow channel 34 and the second mixing buffer area 35, and then flow out of the mixing component 1 through the outlet flow channel 32. When the material to be mixed enters the mixing reaction flow channel 34, the material to be mixed collides with the static mixing structure 40 for multiple times, so that the flow direction of the material can be changed, and in the process, the fluid is more uniform in a mixing manner, so that the material mixing effect can be improved, and the mixing efficiency of the mixing component 1 is improved.

Through the above setting, owing to add static mixed structure 40 in mixing reaction runner 34, the material flow in mixing reaction runner 34 is more disorderly, can solve the material and mix inhomogeneous like this or the velocity of flow is inhomogeneous, unstable problem to reach higher mixing homogeneity, can also improve the mixing efficiency of material simultaneously. In addition, since the fluid is more uniformly mixed by the static mixing structure 40, the stroke of the fluid passing through the mixing reaction channel 34 can be shortened, that is, the size of the mixing reaction channel 34 can be reduced, thereby reducing the volume of the entire mixing assembly 1 and realizing further optimization of space and structure.

It should be noted that the static mixing structure 40 is a static component, i.e., a component that does not move in the mixing reaction channel 34, and by providing the static mixing structure 40, the fluid can be impacted and changed in flow direction for many times, so as to achieve the effect of uniform mixing.

It should be noted that, the mixing module 1 of the present invention has been performed with an experiment simulating the mixing of water and ethanol fluid, and it can be known from the experiment that at the junction of two pipelines entering the flow channel 31, there is an obvious boundary layer between water and ethanol fluid and the phenomenon that ethanol diffuses into water exists; the mixed solution passes through the mixing reaction channel 34 and the plurality of static mixing structures 40 to achieve the effect of uniform mixing. Therefore, the mixing effect of the mixing component 1 of the utility model is very good, the effect of the mixing and feeding of the monomer & activator and the clamping A & clamping B in the oligonucleotide synthesis process to reach the expected mixing ratio can be realized, and the mixing precision and the reproducibility can be controlled under the cooperative cooperation of the monitoring system to ensure the quality and the mass distribution of the final product. The static mixing structure 40 can be superimposed according to the mixing time requirement, and can be adapted to the purpose of on-demand production.

As shown in fig. 3, in the first embodiment of the present invention, the mixing reaction channel 34 includes a first channel groove 341 formed on the upper reaction plate 10 and a second channel groove 342 formed on the lower reaction plate 20, the first channel groove 341 and the second channel groove 342 are arranged to intersect with each other, and a static mixing structure 40 is provided in at least one of the first channel groove 341 and the second channel groove 342.

In the above technical solution, the first flow channel groove 341 is a first bent flow channel groove, the second flow channel groove 342 is a second bent flow channel groove, and the first bent flow channel groove and the second bent flow channel groove are both plural; the plurality of first bent flow channel grooves and the plurality of second bent flow channel grooves are arranged at intervals in the direction from the first mixing buffer zone 33 to the second mixing buffer zone 35, and the bending directions of the respective first bent flow channel grooves and the respective second bent flow channel grooves are opposite.

Through the arrangement, the bent flow channel can change the flow direction of fluid in the flow channel, so that the velocity gradient of the laminar flow movement of the fluid is further increased or a vortex is formed, and the material is mixed more uniformly. Meanwhile, the pressure drop in the flowing process is effectively reduced by the staggered multiple channels, and the flow speed and the flow of each channel can be reasonably distributed by reasonably designing the width and the length of the channel.

As shown in fig. 3, in the first embodiment of the present invention, the static mixing structure 40 located in the first meandering channel and the static mixing structure 40 located in the second meandering channel intersect with each other.

Through the above arrangement, when the fluid is at the end of the channel in the first flow channel 341 or the second flow channel 342, the upper and lower fluids are respectively baffled and respectively opposed or shunted, and are mixed again, so that the uniformity of mixing can be improved, and the material is converged to the second mixing buffer area 35 again after being baffled, opposed or shunted for a plurality of times, thereby completing a mixing unit.

As shown in fig. 4, in the first embodiment of the present invention, the static mixing structure 40 includes a plurality of spiral segments including a first spiral segment 41 and a second spiral segment 42, each of the first spiral segment 41 and the second spiral segment 42 is formed by twisting a rectangular plate by 180 °, and the first spiral segment 41 and the second spiral segment 42 are alternately arranged.

In the above technical solution, the first spiral section 41 and the second spiral section 42 each include a dividing edge 43 at the front end and a drainage edge 44 at the rear end, and an included angle is formed between the dividing edge 43 of the first spiral section 41 and the drainage edge 44 of the second spiral section 42.

Through the arrangement, the spiral section formed by twisting the rectangular plate by 180 degrees has larger contact area, can be fully contacted and collided with the material to be mixed, and the flow direction of the material can be changed for many times by the arrangement, so that the uniformity of material mixing can be further enhanced.

Preferably, the dividing edge 43 of the first helical section 41 is at 90 ° to the drainage edge 44 of the second helical section 42.

In an alternative embodiment of the present invention, the static mixing structure 40 may be further configured such that the static mixing structure 40 includes a central shaft and a plurality of blades disposed on the central shaft, the plurality of blades being spaced apart along an axial direction of the central shaft, the blades being disposed at an angle to the central shaft.

In the above technical solution, the plurality of blades are arranged at intervals around the central shaft in a spiral manner, so that when the fluid enters the mixing reaction channel 34, the flow direction can be changed for many times under the blocking action of the plurality of blades, thereby achieving the effect of uniform mixing.

As shown in fig. 1 and 5, the present invention also provides a synthesizer apparatus, which includes a feeding unit 2 and a mixing assembly 1 connected to the feeding unit 2, wherein the mixing assembly 1 is the above-mentioned mixing assembly 1.

Through the setting, the material to be mixed can enter the mixing assembly 1 through the feeding unit 2, and the mixing assembly 1 is fully and uniformly mixed, so that the material synthesis effect is realized.

As shown in fig. 5, in the first embodiment of the present invention, the feeding unit 2 includes a plurality of first feeding valve groups 23, a plurality of second feeding valve groups 24 correspondingly connected to the plurality of first feeding valve groups 23, and a plurality of control valves 90, each control valve 90 is disposed between a first feeding valve group 23 and a corresponding second feeding valve group 24, the feeding unit 2 further includes a conveying pipeline 25 for connecting the plurality of control valves, so that the materials entering through the first feeding valve group 23 and the second feeding valve group 24 are output through the conveying pipeline 25, the feeding unit 2 further includes an auxiliary cleaning pipeline 80, and the auxiliary cleaning pipeline 80 is connected to one control valve 90 located at the outermost side of the plurality of control valves 90.

In the technical scheme, by arranging the plurality of first feeding valve banks 23 and the plurality of second feeding valve banks 24, materials to be mixed can enter the conveying pipeline 25 through the first feeding valve banks 23 and the second feeding valve banks 24 respectively and are output to the mixing assembly 1 through the conveying pipeline 25, so that the problems of difficulty in cleaning and poor quality of finished products caused by the fact that a plurality of materials are input through the same valve bank and a channel can be solved, and the synthesis effect and the working efficiency of the synthesizer can be improved; a control valve 90 is arranged between each first feeding valve group 23 and the corresponding second feeding valve group 24, and the control valve 90 can control the on-off of the branch, so as to control the material in the channel to enter.

Through the above-mentioned setting, increase an solitary supplementary washing pipeline 80 on the basis of original zero static valves, like this, treat that the misce bene (for example once monomer, reagent, activator or deprotection reagent) carries out abluent in-process to the pipeline behind each feeding valves and the pipeline, supplementary washing pipeline 80 inputs washing reagent in the direction pipeline 25 from the top, can solve because the problem that forms "hydrops" in the pipeline that links to each other with a plurality of control valve 90's top that fluid kinetic energy and pressure energy lead to, avoid this "hydrops" and the next cross contamination between the material, thereby can ensure the purity of misce bene and the quality of final synthetic product. That is to say, through setting up supplementary washing pipeline 80, can wash the pipeline 25 in the middle of having the cross with the working line (for example first inlet valve group 23 and second inlet valve group 24) simultaneously, avoid not working but with the problem that the working line UNICOM department has material cross contamination.

As shown in fig. 5, in the first embodiment of the present invention, the first and second feed valve groups 23 and 24 include a first feed line 21, a feed valve a, a main purge line 27, and a purge on-off valve b. Wherein, the first feeding pipeline 21 arranged on the first feeding valve group 23 is connected with the corresponding second feeding valve group 24 through the control valve 90, and the feeding valve a is arranged on the first feeding pipeline 21; the main purge line 27 is connected to the first feed line 21, and a purge on-off valve b is provided on the main purge line 27.

In the technical scheme, the materials to be mixed enter the conveying pipeline 25 through the first feeding pipeline 21, and the feeding valve a can control the on-off of the first feeding pipeline 21, so that the effect of controlling the branch to start feeding or stop feeding is realized; cleaning reagent can be imported into the conveying pipeline 25 from both sides of the conveying pipeline 25 through the main cleaning pipeline 27, so that pipelines connected with both sides of the plurality of control valves 90 can be cleaned, and thus, under the cooperation of the auxiliary cleaning pipeline 80, cleaning of the control valves 90 without dead angles can be realized, cross contamination between materials which are left and are caused next time is avoided, and the purity of mixed materials and the quality of final synthetic products are ensured. The main cleaning line 27 is provided with a cleaning on-off valve b, which can control the on-off of the main cleaning line 27.

As shown in fig. 5, in the first embodiment of the present invention, the synthesizer apparatus further includes an on-off valve d disposed on the auxiliary cleaning pipeline 80, so that an effect of controlling on-off of the auxiliary cleaning pipeline 80 can be achieved.

In the first embodiment of the present invention, the synthesizer apparatus further includes a control system, and the first feeding valve set 23, the second feeding valve set 24, and the control valve 90 are all connected to the control system.

In the above technical solution, the feeding valve a, the cleaning on-off valve b, the plurality of control valves 90 and the on-off valve d are all connected with the control system, so that each channel and branch of the feeding unit 2 can be controlled by the control system according to actual use requirements.

Through the arrangement, when feeding is needed, the control system controls the feeding valve a on the corresponding branch and the control valve 90 arranged on the conveying pipeline 25 to be in the opening state, at the moment, the on-off valve d and the cleaning on-off valve b are in the closing state, and materials to be mixed can enter the mixing assembly 1 through the branch and the conveying pipeline 25 through the feeding unit 2; when feeding is completed and the feeding unit 2 needs to be fed, the on-off valve d and the cleaning on-off valve b are opened through the control system, and the feeding valve a is closed, so that cleaning agents can enter the control valve 90 through each branch, each channel connected with the control valve 90 is cleaned, and the next feeding process can be started under the control of the control system after the cleaning is completed. Therefore, the fluid of each branch can be prevented from interfering with each other by controlling the on-off of each branch, so that the purity of the mixed material and the quality of the final synthesized product are ensured.

As shown in fig. 6, in the first embodiment of the present invention, the synthesizer apparatus further includes a synthesis column assembly 60, the synthesis column assembly 60 includes a synthesis column 61 and a back-flushing device 62 connected to the synthesis column 61, the synthesis column 61 has a reaction chamber 63 and a first inlet 64 and a first outlet 65 both communicated with the reaction chamber 63, and the back-flushing device 62 is used for back-flushing the reaction chamber 63 from the first outlet 65 to the first inlet 64.

In the above technical solution, the synthesis column assembly 60 further includes an inflow pipeline and an outflow pipeline, wherein the inflow pipeline includes a first pipeline and a second pipeline connected with each other, and the second pipeline is communicated with the first inlet 64; the discharge line includes a third line and a fourth line connected, the third line communicating with the first outlet 65. The mixed material flows through the first pipeline and the second pipeline in sequence, enters the reaction cavity 63 through the first inlet 64, completes the synthetic reaction in the reaction cavity 63, and the product obtained by the synthetic reaction is discharged through the third pipeline and the fourth pipeline.

In the reaction chamber 63, the mixed material has a forward motion trajectory from the first inlet 64 to the first outlet 65; the liquid flows from the middle in the radial direction and the longitudinal direction, the resin middle is more and more sunken due to the impact of the liquid flow in the later synthesis period, and a back washing device 62 is further arranged in the synthesis column assembly 60, when the back washing device 62 is started, the washing liquid flows in the reaction cavity 63 along the reverse movement track from the first outlet 65 to the first inlet 64, and therefore the distribution of the carrier can be optimized so as to fully utilize all active parts in the reaction cavity 63.

Through the arrangement, the back washing device 62 is additionally arranged around the synthesis column 61, so that the reaction cavity 63 can be back washed regularly, the problem of loss of active sites caused by uneven fluid distribution can be avoided, and the synthesis column assembly 60 is ensured to have high synthesis efficiency.

As shown in fig. 6, in the first embodiment of the present invention, the back-flushing device 62 includes a first flushing line 66 communicated with the first outlet 65 and a second flushing line 67 connected with the first inlet 64, and the synthesizer apparatus further includes a power pump disposed on the first flushing line 66 or the second flushing line 67 to allow the flushing liquid to enter the reaction chamber 63 from the first outlet 65 and flow out from the first inlet 64.

In the above technical scheme, one end of the first flushing pipeline 66 is connected with the first pipeline, the other end of the first flushing pipeline 66 is connected with a connection node between the third pipeline and the fourth pipeline, one end of the second flushing pipeline 67 is connected with the fourth pipeline, and the other end of the second flushing pipeline 67 is connected with a connection node between the first pipeline and the second pipeline.

Through the above arrangement, the flushing liquid can flow through the first flushing pipeline 66 and the second flushing pipeline 67, enter the reaction chamber 63 from the first outlet 65 and flow out from the first inlet 64, so that the flushing liquid can perform back flushing on the reaction chamber 63, and all active sites in the reaction chamber 63 can be fully utilized.

As shown in fig. 6, in the first embodiment of the present invention, the synthesizer apparatus further comprises a three-way valve assembly, wherein a first three-way valve is provided at a connection node of the first flushing line 66 and the first line; the second three-way valve is arranged at the connecting node of the first flushing pipeline 66, the third pipeline and the fourth pipeline; a third three-way valve provided at a connection node of the second flushing line 67, the first line, and the second line; and a fourth three-way valve provided at a connection node of the second flushing line 67 and the fourth line.

Through the setting, set up the three-way valve between each pipeline of intercommunication each other, can realize the two-way flow of fluid in the pipeline to realize that the mixed material forward flows and the effect of flushing agent back flush.

Example two

Fig. 7 shows a schematic structural diagram of a second embodiment of the composite column assembly, and the second embodiment of the utility model only differs from the first embodiment in that: in the second embodiment, the flow direction of the mixed material in the synthesis column assembly 60 is different from that in the first embodiment, specifically, the mixed material flows in the synthesis column 61 in the direction of going in and out.

The arrangement of the rest of the composite column assembly is the same as the embodiment shown in fig. 6 and will not be described again here. The inventors carried out tests using the synthesizer apparatus of this example, and this test synthesized 22 monomers, and the sequence of oligonucleotide chain synthesis was 5'-TGACTGTGAACGTTCGAGATGA-3', 6A, 3C, 7G, and 6T.

The test process is as follows:

(1) equipment debugging: pre-knockout, and confirming that the equipment can normally run;

(2) preparing: batching, filling a column and measuring water content;

(3) synthesizing: automatically feeding according to a given synthesis sequence (deprotection, cleaning, activation, cleaning, oxidation/thio, cleaning, end capping and cleaning, and sequentially coupling each monomer in the sequence until a given length of a target oligonucleotide chain is reached) to perform oligonucleotide synthesis;

(4) cutting: the complete nucleotide chain is peeled off from the support.

The above is a general procedure for testing by using a synthesizer, and after cutting, product post-treatment and purity detection are required. According to the results of the examination, the Oligo (oligonucleotide) synthesized by the synthesizer apparatus of this example had a purity of 73.49%, which was superior to that in the related art (about 65%).

From the above description, it can be seen that the above-described embodiments of the present invention achieve the following technical effects: the material flow in the mixed reaction runner is more disordered, so that the problems of uneven material mixing or uneven flow rate and instability can be solved, higher mixing uniformity is achieved, and the mixing efficiency of the materials can be improved. In addition, because the fluid is mixed more uniformly under the action of the static mixing structure, the size of the mixing reaction flow channel can be reduced, so that the volume of the whole mixing component is reduced, and the further optimization of the space and the structure is realized; meanwhile, the pressure drop in the flowing process is effectively reduced by the staggered multiple channels, the flow speed and the flow of each channel are reasonably distributed by reasonably designing the width and the length of the channel, and efficient heat and mass transfer is realized in the channel. The spiral section has bigger area of contact, can fully contact and collide with the material that waits to mix to can further strengthen the homogeneity that the material mixes. An independent auxiliary cleaning pipeline is added on the basis of the original zero static valve group, so that the cross contamination between the residual material and the next material can be avoided, and the purity of the mixed material and the quality of the final synthetic product can be ensured. The back washing device is additionally arranged around the synthesis column, so that the reaction cavity can be back washed periodically, the distribution of the carriers can be optimized so as to fully utilize all active parts in the reaction cavity, the problem of loss of the active parts caused by uneven fluid distribution can be avoided, and the synthesis column assembly is ensured to have higher synthesis efficiency.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (10)

1. A mixing assembly, comprising:

an upper reaction plate (10);

a lower reaction plate (20) superposed with said upper reaction plate (10);

an inlet flow channel (31) and an outlet flow channel (32) disposed between said upper reaction plate (10) and said lower reaction plate (20);

the reaction unit (30) is arranged between the upper reaction plate (10) and the lower reaction plate (20), the reaction unit (30) comprises a first mixing buffer area (33), a mixing reaction flow channel (34) and a second mixing buffer area (35) which are sequentially connected, the first mixing buffer area (33) is communicated with the second mixing buffer area (35) through the mixing reaction flow channel (34), the first mixing buffer area (33) is communicated with the inlet flow channel (31), and the second mixing buffer area (35) is communicated with the outlet flow channel (32);

and the static mixing structure (40) is arranged in the mixing reaction flow channel (34) to increase the speed gradient of the laminar flow movement of the fluid or form a vortex so as to mix the materials to be mixed entering the mixing reaction flow channel (34).

2. The mixing assembly according to claim 1, wherein the static mixing structure (40) comprises a plurality of helical segments including a first helical segment (41) and a second helical segment (42), at least one of the first helical segment (41) and the second helical segment (42) being formed by a rectangular plate twisted by 180 °, the first helical segment (41) and the second helical segment (42) being arranged alternately.

3. The mixing assembly according to claim 2, wherein the first helical section (41) and the second helical section (42) are each made from a rectangular plate twisted 180 °, the first helical section (41) and the second helical section (42) each comprise a dividing edge (43) at a front end and a drainage edge (44) at a rear end, and the dividing edge (43) of the first helical section (41) and the drainage edge (44) of the second helical section (42) have an angle therebetween.

4. The mixing assembly of claim 1, wherein the static mixing structure (40) further comprises a central shaft and a plurality of vanes disposed on the central shaft, the plurality of vanes being spaced apart along an axis of the central shaft, the vanes being disposed at an angle to the central shaft.

5. The mixing assembly according to any one of claims 1 to 4, wherein the mixing reaction flow channel (34) comprises a first flow channel groove (341) provided on the upper reaction plate (10) and a second flow channel groove (342) provided on the lower reaction plate (20), the first flow channel groove (341) and the second flow channel groove (342) being arranged crosswise, the static mixing structure (40) being provided in at least one of the first flow channel groove (341) and the second flow channel groove (342).

6. Synthesizer device, characterized in that it comprises a feeding unit (2) and a mixing assembly (1) connected to the feeding unit (2), the mixing assembly (1) being a mixing assembly according to any one of claims 1 to 5.

7. Synthesizer apparatus according to claim 6, characterized in that the feed unit (2) comprises a plurality of first feed valve blocks (23), a plurality of second feed valve blocks (24) connected in correspondence with the plurality of first feed valve blocks (23), and a plurality of control valves (90), each control valve (90) being arranged between the first feed valve block (23) and the corresponding second feed valve block (24), the feeding unit (2) further comprising a conveying conduit (25) for connecting a plurality of said control valves (90), so that the material entering through the first inlet valve group (23) and the second inlet valve group (24) is output through the conveying pipeline (25), the feeding unit (2) further comprises an auxiliary cleaning pipeline (80), and the auxiliary cleaning pipeline (80) is connected with the outermost one of the control valves (90).

8. Synthesizer apparatus according to claim 7, characterized in that at least one of the first and second inlet valve group (23, 24) comprises: a first feeding line (21) connected to the corresponding second feeding valve group (24) through the control valve (90); a feed valve arranged on the first feed line (21); the synthesizer equipment also comprises a main cleaning pipeline (27) connected with the first feeding pipeline (21) and a cleaning on-off valve arranged on the main cleaning pipeline (27); alternatively, the first and second electrodes may be,

the synthesizer equipment also comprises an on-off valve d arranged on the auxiliary cleaning pipeline (80) so as to control the on-off of the auxiliary cleaning pipeline (80); alternatively, the first and second electrodes may be,

the synthesizer equipment also comprises a control system, and the first feeding valve group (23), the second feeding valve group (24) and the control valve (90) are all connected with the control system.

9. Synthesizer apparatus according to claim 6, further comprising a synthesis column assembly (60), the synthesis column assembly (60) comprising a synthesis column (61) and a back-flushing device (62) connected to the synthesis column (61), the synthesis column (61) having a reaction chamber (63) and a first inlet (64) and a first outlet (65) communicating with both the reaction chamber (63), from the first outlet (65) to the first inlet (64), the back-flushing device (62) being adapted to back-flush the reaction chamber (63).

10. The synthesizer apparatus according to claim 9, wherein the back flushing means (62) comprises a first flushing line (66) communicating with the first outlet (65) and a second flushing line (67) connected to the first inlet (64), the synthesizer apparatus further comprising a power pump arranged on the first flushing line (66) or the second flushing line (67) to let flushing liquid enter the reaction chamber (63) from the first outlet (65) and flow out from the first inlet (64).

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