CN216192251U - Cell mar experimental apparatus - Google Patents
Cell mar experimental apparatus Download PDFInfo
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- CN216192251U CN216192251U CN202122516354.XU CN202122516354U CN216192251U CN 216192251 U CN216192251 U CN 216192251U CN 202122516354 U CN202122516354 U CN 202122516354U CN 216192251 U CN216192251 U CN 216192251U
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Abstract
The utility model relates to a cell scratch experimental device, which comprises a cover body, a scratch device and a culture dish; the cover body is provided with a slideway along the diameter, and one end of the slideway is provided with a spring; the scratching device comprises a handle part and a scratching needle, the scratching needle is detachably connected with the handle part, the handle part comprises a sliding block, an operating head is arranged on the sliding block, one end of the sliding block is connected with a spring, and two sides of the sliding block are attached to the slide way; the cover body is connected with the upper edge of the culture dish. The experimental device for the scratch of the various cells has low requirement on the operation proficiency of experimenters, can accurately, straightly and repeatedly etch the various cell scratches for many times with the same width, can greatly reduce experimental errors and improve experimental efficiency.
Description
Technical Field
The utility model relates to the field of biological experiment equipment, in particular to a cell scratch experiment device.
Background
At present, the prevalence rate of malignant tumors in the population is high, the most prominent characteristic capable of expressing the malignancy degree of the malignant tumors is cell metastasis, one of the key points of the current research of medical researchers is the metastasis of tumor cells, and when the malignant transformation of cells caused by certain adverse factors is determined, a cell scratch experiment is mostly adopted.
The cell scratch test is a simple and easy method for detecting cell movement, has low test cost, and can be used for detecting the migration phenomenon of tumor cells growing adherent to the skin. The general cell scratch experiment procedure is: the method is characterized in that cell culture adherence is carried out in a culture dish or a six-hole plate, after cell density meets the requirements, an experimenter draws a scratch on the bottom of the culture dish by using a pipette head without any support, then periodically observes and shoots, and records the condition that bacteria on two sides of the scratch migrate and grow to the middle. And draw mar operation and need the experimenter to have abundant experience and have exquisite accurate means of operation just can guarantee the accuracy of experimental result, if operate improperly, some errors that are difficult to avoid such as mar slope, mar breakpoint and width are different can appear to among the current cell mar experimental apparatus, it is comparatively loaded down with trivial details to make, and market value is not big.
Therefore, it is necessary to design a device that is easy to operate and can accurately, equally wide and straightly perform the cell scratch test.
SUMMERY OF THE UTILITY MODEL
Therefore, the utility model aims to solve the technical problem that the cell scratching experiment device in the prior art can not finish scratching operation in an equal width, straight and accurate manner and has higher requirement on the proficiency of experimenters.
In order to solve the technical problem, the utility model provides a cell scratch experimental device, which comprises a cover body, a scratch device and a culture dish;
the cover body is provided with a slide way along the diameter, and one end of the slide way is provided with a spring;
the scratching device comprises a handle part and a scratching needle, the scratching needle is detachably connected with the handle part, the handle part comprises a sliding block, an operating head is arranged on the sliding block, one end of the sliding block is connected with the spring, and two sides of the sliding block are attached to the slide way;
the cover body is connected with the upper edge of the culture dish.
In one embodiment of the utility model, the pressing line base is provided with a clamping edge, and the slide way is a rectangular hole with one open end.
In one embodiment of the present invention, the cover body is provided with a groove inward along the slide way, and the size of the groove is matched with that of the slide block.
In an embodiment of the present invention, the cover body is provided with a notch on the opening side of the slide way, and the size of the notch is matched with the size of the slide block.
In one embodiment of the utility model, the slider is provided with a spring connector, and the spring connector is detachably connected with the spring.
In one embodiment of the utility model, the operating head is higher than the cover surface.
In one embodiment of the utility model, the operating head has a cross-section smaller than the cross-section of the slider.
In one embodiment of the present invention, the thickness of the cover body is such that the tip of the scratching needle is in vertical contact with the bottom of the culture dish.
In one embodiment of the utility model, the cover is provided with a clip, which is tightly attached to the upper edge of the culture dish.
In one embodiment of the present invention, the tip of the scribing needle is made of rubber.
Compared with the prior art, the technical scheme of the utility model has the following advantages:
the cell scratching experimental device has low requirement on the operation proficiency of experimenters, can accurately, straightly and repeatedly etch and finish multiple cell scratching operations with equal width, can greatly reduce experimental errors and improve experimental efficiency.
Drawings
In order that the present disclosure may be more readily and clearly understood, reference is now made to the following detailed description of the embodiments of the present disclosure taken in conjunction with the accompanying drawings, in which
Fig. 1 is a top view of the present invention.
Fig. 2 is a front view of the present invention.
Fig. 3 is a top view of the utility model applied to a six-orifice plate.
The specification reference numbers indicate: 1. a cover body; 2. a culture dish; 3. a scratching device; 301. a handle; 302. a scratching needle; 311. an operating head; 312. a slider; 4. a slideway; 401. a groove; 5. a spring; 6. a spring connector; 7. a notch; 8. clamping a hoop; 9. six orifice plates.
Detailed Description
The present invention is further described below in conjunction with the following figures and specific examples so that those skilled in the art may better understand the present invention and practice it, but the examples are not intended to limit the present invention.
Referring to fig. 1 and 2, the present invention provides a cell scratching experiment device, which comprises a cover body 1, a scratching device 3 and a culture dish 2; a slide way 4 is arranged along the diameter of the cover body 1, and a spring 5 is arranged at one end of the slide way 4; the scratching device 3 comprises a handle part 301 and a scratching needle 302, the scratching needle 302 is detachably connected with the handle part 301, the whole device is convenient to use repeatedly after being sterilized, the handle part 301 comprises a sliding block 312, an operating head 311 is arranged on the sliding block 312, one end of the sliding block 312 is connected with a spring 5, and two sides of the sliding block 312 are attached to the slide way 4; the cover 1 is connected to the upper edge of the culture dish 2. The slideway 4 is a rectangular hole with one end open.
With continued reference to fig. 1 and 2, the cover body 1 is provided with a groove 401 inwards along the slide way 4, the size of the groove 401 is matched with that of the slide block 312, the groove 401 is symmetrical along the slide way 4 and just can accommodate the slide block 312 of the handle 301 of the scratcher 3, so that the stability of the scratcher 3 in the sliding process is ensured, the groove 401 can slide along the track of the groove 401, and the problems of skew scratch and thickness inequality caused by left-right shaking or up-down shaking in manual operation can be prevented.
The slide block 312 is provided with a spring connector 6, and the spring connector 6 is detachably connected with the spring 5. Spring 5's existence provides certain degree ground pulling force for scratcher 3 in the slip in-process, and the straightness of mar has also been guaranteed to the pulling force directionality to can prevent that scratcher 3 from leading to the breakpoint of mar at the pause of slip in-process, make things convenient for the laborsaving high-efficient operation of accomplishing of operator.
The operating head 311 is higher than the surface of the cover body 1, which is convenient for the experimenter to operate. While the cross-section of the operating head 311 is smaller than that of the slider 312 in order to ensure that the slider 312 can be inserted into the recess 401 without jumping up and down.
The tip of lid 1's thickness should guarantee that mar needle 302 and the perpendicular contact in bottom of culture dish 2, and the tip of while mar needle 302 is rubber material, and this department uses rubber material to be used for increasing frictional force and contact force between tip and culture dish 2 to make the removal of the bacterium in the region that sliding contact passed the very big degree. Specifically, the scratch needles 302 with the same size can realize the equal thickness of the porous scratch, so that the later photographing and observation comparison is facilitated, and the scratch needles 302 with different thicknesses can also be used for realizing the change of the thickness of the scratch;
Referring to fig. 3, the culture dish 2 can be replaced by a six-hole plate 9, the multi-group cover body 1 and the scratcher 3 can be operated simultaneously or the cell scratching operation can be performed in a multi-group multi-operation mode, multi-group experimental results can be observed more intuitively, experimental errors are reduced, and the experimental efficiency is improved.
During operation, the cover body 1 is covered on the culture dish 2 or the six-hole plate 9, and the handle part 301 of the scratching device 3 and the scratching needle 302 are assembled; then the other end of the spring 5 fixed in the upper slide way 4 of the cover body 1 is connected with one end of the handle part 301, the operating head 311 on the top of the scratching device 3 is pinched and moved to the notch 7 of the cover body 1, the scratching device 3 is placed downwards, the sliding block 312 is embedded into the groove 401, at the moment, the spring 5 is in a stretching state, the scratching needle 302 is vertical to the cover body 1, and the tip part is tightly attached to the bottom of the culture dish 2 or the six-hole plate 9; releasing at the uniform velocity makes scratcher 3 slide to spring 5 and be in the natural extension state, takes off lid 1 and scratcher 3 at last, accomplishes the mar operation.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications of the utility model may be made without departing from the spirit or scope of the utility model.
Claims (10)
1. The utility model provides a cell mar experimental apparatus which characterized in that: comprises a cover body, a scratcher and a culture dish;
the cover body is provided with a slide way, and one end of the slide way is provided with a spring;
the scratching device comprises a handle part and a scratching needle, the scratching needle is detachably connected with the handle part, the handle part comprises a sliding block, an operating head is arranged on the sliding block, one end of the sliding block is connected with the spring, and two sides of the sliding block are attached to the slide way;
the culture dish is connected with the cover body.
2. The cell scratch experimental apparatus according to claim 1, wherein: the slide is arranged along the diameter of the cover body, and the slide is a rectangular hole channel with one open end.
3. The cell scratch experimental apparatus according to claim 1, wherein: the cover body is provided with a groove inwards along the slide way, and the size of the groove is matched with that of the sliding block.
4. The cell scratch experimental apparatus according to claim 1, wherein: the lid is in the opening side of slide is provided with the breach, the breach size with slider size adaptation.
5. The cell scratch experimental apparatus according to claim 1, wherein: the slider is provided with the spring coupling head, the spring coupling head with the detachable connection of spring.
6. The cell scratch experimental apparatus according to claim 1, wherein: the operating head is higher than the surface of the cover body.
7. The cell scratch experimental apparatus according to claim 1, wherein: the cross section of the operating head is smaller than that of the sliding block.
8. The cell scratch experimental apparatus according to claim 1, wherein: the thickness of the cover body is configured to ensure that the tip of the scratching needle is in vertical contact with the bottom of the culture dish.
9. The cell scratch experimental apparatus according to claim 1, wherein: the lid is provided with the clamp, the clamp closely laminates with the last edge of culture dish.
10. The cell scratch experimental apparatus according to claim 1, wherein: the sharp part of mar needle is the rubber material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122516354.XU CN216192251U (en) | 2021-10-19 | 2021-10-19 | Cell mar experimental apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122516354.XU CN216192251U (en) | 2021-10-19 | 2021-10-19 | Cell mar experimental apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216192251U true CN216192251U (en) | 2022-04-05 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122516354.XU Active CN216192251U (en) | 2021-10-19 | 2021-10-19 | Cell mar experimental apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216192251U (en) |
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2021
- 2021-10-19 CN CN202122516354.XU patent/CN216192251U/en active Active
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