CN216095388U

CN216095388U - Fluorescence quantitative PCR detection kit for enterogaster hepatica in water

Info

Publication number: CN216095388U
Application number: CN202122405995.8U
Authority: CN
Inventors: 牟潜; 赵久莲; 邱梅; 杨淑娜
Original assignee: Qingdao Future Detection Co ltd
Current assignee: Qingdao Future Detection Co ltd
Priority date: 2021-09-30
Filing date: 2021-09-30
Publication date: 2022-03-22
Anticipated expiration: 2031-09-30

Abstract

The utility model relates to the technical field of kits, and discloses a fluorescence quantitative PCR detection kit for enterobacter hepatica in water, which solves the problem that test tubes are too cumbersome to wash independently and comprises a detection box, wherein a heating cavity is formed in one side of the interior of the detection box, a heating coil is arranged in the middle of the heating cavity, a fixed chuck is arranged at the top end of the detection box, the test tubes are arranged on the outer side of the fixed chuck at equal angles, a top cover is arranged at the top end of the heating cavity and comprises a detection cover and a cleaning cover, the detection cover and the cleaning cover can be replaced with each other, a fixed water inlet mechanism is arranged at the top end of the cleaning cover, a cleaning mechanism is arranged at the top end of the cleaning cover, and the fixed water inlet mechanism comprises a water inlet ring arranged at the top end of the cleaning cover; in the utility model, in operation, the gear ring is driven to rotate by the arranged motor and is meshed with the gears, so that the motor drives the cleaning devices to clean the inner walls of the test tubes, the cleaning efficiency is improved, and the cleaning and sorting time is saved.

Description

Fluorescence quantitative PCR detection kit for enterogaster hepatica in water

Technical Field

The utility model belongs to the technical field of kits, and particularly relates to a fluorescence quantitative PCR detection kit for enterobacter hepatica in water.

Background

In the conventional PCR, the amplified product is analyzed and detected by an end-point method, i.e., after the PCR reaction is finished, DNA is electrophoresed through agarose gel and then is subjected to imaging analysis, and the fluorescence quantitative PCR can be used for analyzing and detecting the accumulated amplified product in the reaction process, i.e., real-time detection.

Because in the fluorescence quantitative PCR detection, need will detect liquid and put into the test tube, after detecting, need wash the test tube, because the test tube number is more, wash complex operation alone.

SUMMERY OF THE UTILITY MODEL

Aiming at the situation and overcoming the defects of the prior art, the utility model provides the fluorescence quantitative PCR detection kit for the enterobacter hepatica in water, which effectively solves the problem that the test tube is too complicated to be cleaned independently.

In order to achieve the purpose, the utility model provides the following technical scheme: a fluorescence quantitative PCR detection kit for enterobacter hepatica in water comprises a detection box, wherein a heating cavity is formed in one side of the interior of the detection box, a heating coil is installed in the middle of the heating cavity, a fixed chuck is installed at the top end of the detection box, test tubes are installed on the outer side of the fixed chuck at equal angles, and a top cover is installed at the top end of the heating cavity;

the top cap divide into and detects lid and washing lid, detects the lid and can replace each other with the washing lid, and the fixed mechanism of intaking is installed on the top of washing the lid, and clean mechanism is installed on the top of washing the lid.

Preferably, fixed mechanism of intaking is including installing in the ring of intaking on washing lid top, and the inlet tube is installed to the top symmetry of the ring of intaking, and the outlet pipe is installed to angle such as the bottom of the ring of intaking, and the draw-in groove that is located the outlet pipe below is seted up to angle such as the top of washing lid, and the locating lever is installed to angle such as washing lid bottom, and the constant head tank has been seted up to the inside of fixing chuck and the bottom of heating chamber angle such as, and locating lever and constant head tank are corresponding.

Preferably, a plurality of communicating grooves are formed in the water inlet ring, and the water inlet pipe is communicated with the water outlet pipe through the communicating grooves.

Preferably, the locating lever includes that the equidistance is installed in the kelly of cleaning lid bottom, and the spring is installed to the kelly bottom, and the fixture block is installed to the spring bottom, detects lid and cleaning lid and all passes through bolt fixed connection with the heating chamber top.

Preferably, clean mechanism is including installing in the ring gear on washing lid top, and the motor is installed to washing lid top, and motor output gear meshes with the ring gear outside mutually, and the internally mounted of draw-in groove has the sleeve pipe, and the gear that is located washing lid top is installed on sheathed tube top, and the gear meshes with the ring gear inboard mutually, and the clean feeler is installed to sheathed tube outside equidistance, and clean hole has been seted up to the angle such as sheathed tube outside.

Preferably, the top end of the sleeve is movably sleeved with the bottom end of the water outlet pipe, and adjacent gears are not in contact.

Preferably, the cleaning contact pins and the cleaning holes are arranged on the outer side of the sleeve at intervals in a crossed manner, and the outer ends of the cleaning contact pins are in contact with the inner wall of the test tube.

Compared with the prior art, the utility model has the beneficial effects that:

1) in the work, the gear ring is driven to rotate by the arranged motor and is meshed with the gears, so that the motor drives the cleaning devices to clean the inner walls of the test tubes, the cleaning efficiency is improved, and the cleaning and sorting time is saved;

2) the utility model discloses a test tube cleaning device, including the casing pipe, the casing pipe top that sets up is cup jointed with the outlet pipe at work for the casing pipe is rotating to carry out the test tube inner wall cleanness and is intake from clean hole simultaneously, makes clean feeler clean more thoroughly to the inner wall of test tube, thereby improves the cleaning performance.

Drawings

The accompanying drawings, which are included to provide a further understanding of the utility model and are incorporated in and constitute a part of this specification, illustrate embodiments of the utility model and together with the description serve to explain the principles of the utility model and not to limit the utility model.

In the drawings:

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view of the cleaning cover of the present invention;

FIG. 3 is a schematic structural view of a fixed water inlet mechanism of the present invention;

fig. 4 is a schematic structural diagram of the cleaning mechanism of the present invention.

In the figure: 1. a detection cartridge; 2. a heating cavity; 3. a heating coil; 4. fixing the chuck; 5. a test tube; 6. a top cover; 6a, a detection cover; 6b, cleaning the cover; 7. fixing a water inlet mechanism; 701. a water inlet ring; 702. a water inlet pipe; 703. a water outlet pipe; 704. a card slot; 705. positioning a rod; 706. positioning a groove; 8. a cleaning mechanism; 801. a toothed ring; 802. a motor; 803. a sleeve; 804. a gear; 805. cleaning the contact pins; 806. the hole is cleaned.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments; all other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the first embodiment, as shown in fig. 1 to 4, the detection kit comprises a detection kit 1, a heating cavity 2 is formed in one side of the interior of the detection kit 1, a heating coil 3 is installed in the middle of the heating cavity 2, a fixing chuck 4 is installed at the top end of the detection kit 1, test tubes 5 are installed on the outer side of the fixing chuck 4 at equal angles, a top cover 6 is installed at the top end of the heating cavity 2, the top cover 6 is divided into a detection cover 6a and a cleaning cover 6b, the detection cover 6a and the cleaning cover 6b can be replaced with each other, a fixed water inlet mechanism 7 is installed at the top end of the cleaning cover 6b, and a cleaning mechanism 8 is installed at the top end of the cleaning cover 6 b.

In the second embodiment, on the basis of the first embodiment, the fixed water inlet mechanism 7 includes a water inlet ring 701 installed at the top end of the cleaning cover 6b, water inlet pipes 702 are symmetrically installed at the top end of the water inlet ring 701, water outlet pipes 703 are installed at equal angles at the bottom end of the water inlet ring 701, a clamping groove 704 located below the water outlet pipes 703 is installed at equal angles at the top end of the cleaning cover 6b, positioning rods 705 are installed at equal angles at the bottom end of the cleaning cover 6b, positioning grooves 706 are installed at equal angles inside the fixing chuck 4 and the bottom end of the heating chamber 2, the positioning rods 705 correspond to the positioning grooves 706, a plurality of communicating grooves are formed inside the water inlet ring 701, the water inlet pipes 702 are communicated with the water outlet pipes 703 through the communicating grooves, the positioning rods 705 include clamping rods installed at equal angles at the bottom end of the cleaning cover 6b, springs are installed at the bottom ends of the clamping rods, the detecting cover 6a and the cleaning cover 6b are both fixedly connected with the top end of the heating chamber 2 through bolts, when the test finishes, need carry out the cleanness of test tube 5, will detect and cover 6a and take off, cover cleaning cover 6b and pass through bolt and detection box 1 top fixed connection, in the installation with cleaning cover 6b, insert the back from constant head tank 706 on fixing chuck 4 with the locating lever 705 that cleaning cover 6b bottom, and fix in the constant head tank 706 of heating chamber 2 bottom, owing to accomplish the location this moment, make the sleeve pipe 803 of cleaning cover 6b bottom can be accurate insert in the test tube 5.

Third embodiment, on the basis of the second embodiment, the cleaning mechanism 8 includes a toothed ring 801 installed on the top end of the cleaning cover 6b, a motor 802 is installed on the top end of the cleaning cover 6b, an output gear of the motor 802 is engaged with the outer side of the toothed ring 801, a sleeve 803 is installed inside the clamping groove 704, a gear 804 located above the cleaning cover 6b is installed on the top end of the sleeve 803, the gear 804 is engaged with the inner side of the toothed ring 801, cleaning contact pins 805 are installed on the outer side of the sleeve 803 at equal intervals, cleaning holes 806 are formed on the outer side of the sleeve 803 at equal angles, the top end of the sleeve 803 is movably sleeved with the bottom end of the water outlet pipe 703, the adjacent gear 804 is not in contact, the cleaning contact pins 805 and the cleaning holes 806 are alternately arranged on the outer side of the sleeve 803, the outer end of the cleaning contact pins is in contact with the inner wall of the test tube 5, cleaning water is introduced from the water inlet pipe 702, the cleaning water is introduced from the water inlet pipe 702 through the communication groove inside the water inlet ring 701, and the sleeve 803 is sleeved outside the water outlet pipe 703, so that the cleaning water enters the sleeve 803, the motor 802 is started, the output gear of the motor 802 is meshed with the gear ring 801, the inner side of the gear ring 801 is meshed with the gear 804, so that the output gear of the motor 802 rotates to drive the plurality of gears 804 to rotate, at the moment, the cleaning contact pins 805 outside the bottom end of the sleeve 803 contact with the inner wall of the test tube 5 for cleaning, and meanwhile, the cleaning water introduced into the sleeve 803 is discharged from the cleaning holes 806 for cleaning.

The working principle is as follows: when the detection box 1 works, firstly, when detection is carried out in the detection box 1, a detection reagent is added into the test tube 5, the detection cover 6a is covered, the detection cover 6a is connected with the top end of the detection box 1 through a bolt, and the heating coil 3 is started to heat the test tube 5 so as to carry out detection;

when the test tube 5 needs to be cleaned after the test is finished, the test cover 6a is taken down, the cleaning cover 6b is covered and fixedly connected with the top end of the test box 1 through a bolt, in the process of installing the cleaning cover 6b, a positioning rod 705 at the bottom end of the cleaning cover 6b is inserted from a positioning groove 706 on the fixed chuck 4 and is fixed in a positioning groove 706 at the bottom end of the heating cavity 2, and at the moment, due to the completion of the positioning, the sleeve 803 at the bottom end of the cleaning cover 6b can be accurately inserted into the test tube 5;

after the fixing, clean water is introduced from the water inlet pipe 702, the clean water is introduced from the water inlet pipe 702 through the communicating groove inside the water inlet ring 701 and is discharged from the water outlet pipe 703, the outer side of the water outlet pipe 703 is sleeved with the sleeve 803, so that the clean water enters the sleeve 803, the motor 802 is started, because the output gear of the motor 802 is meshed with the toothed ring 801, the inner side of the toothed ring 801 is meshed with the gear 804, so that the output gear of the motor 802 rotates to drive the gears 804 to rotate, at the moment, the cleaning contact pin 805 outside the bottom end of the sleeve 803 is contacted with the inner wall of the test tube 5 for cleaning, and the clean water introduced into the sleeve 803 is introduced from the cleaning hole 806 for cleaning.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The fluorescence quantitative PCR detection kit for the enterobacter hepatica in water comprises a detection kit (1) and is characterized in that: a heating cavity (2) is formed in one side of the interior of the detection box (1), a heating coil (3) is installed in the middle of the heating cavity (2), a fixed chuck (4) is installed at the top end of the detection box (1), test tubes (5) are installed on the outer side of the fixed chuck (4) at equal angles, and a top cover (6) is installed at the top end of the heating cavity (2);

top cap (6) divide into and detect lid (6a) and washing lid (6b), detect lid (6a) and washing lid (6b) and can replace each other, wash the top of lid (6b) and install fixed mechanism (7) of intaking, wash the top of lid (6b) and install clean mechanism (8).

2. The fluorescence quantitative PCR detection kit for the enterobacter hepatica in water according to claim 1, characterized in that: fixed mechanism (7) of intaking is including installing in the ring of intaking (701) on washing lid (6b) top, inlet tube (702) are installed to the top symmetry of ring of intaking (701), outlet pipe (703) are installed to angles such as the bottom of ring of intaking (701), draw-in groove (704) that are located outlet pipe (703) below are seted up to angles such as the top of washing lid (6b), locating lever (705) are installed to angles such as washing lid (6b) bottom, locating slot (706) have been seted up to the inside of fixed chuck (4) and the bottom of heating chamber (2) impartial angle, locating lever (705) and locating slot (706) are corresponding.

3. The fluorescence quantitative PCR detection kit for the enterobacter hepatica in water according to claim 2, characterized in that: a plurality of communicating grooves are formed in the water inlet ring (701), and the water inlet pipe (702) is communicated with the water outlet pipe (703) through the communicating grooves.

4. The fluorescence quantitative PCR detection kit for the enterobacter hepatica in water according to claim 2, characterized in that: the locating rod (705) comprises a clamping rod which is arranged at the bottom end of the cleaning cover (6b) at an equal angle, a spring is arranged at the bottom end of the clamping rod, a clamping block is arranged at the bottom end of the spring, and the detection cover (6a) and the cleaning cover (6b) are fixedly connected with the top end of the heating cavity (2) through bolts.

5. The fluorescence quantitative PCR detection kit for the enterobacter hepatica in water according to claim 1, characterized in that: clean mechanism (8) are including installing in ring gear (801) on washing lid (6b) top, wash lid (6b) top and install motor (802), motor (802) output gear meshes with ring gear (801) outside mutually, the internally mounted of draw-in groove (704) has sleeve pipe (803), gear (804) that are located washing lid (6b) top are installed on the top of sleeve pipe (803), gear (804) mesh with ring gear (801) inboard mutually, clean touching foot (805) are installed to the outside equidistance of sleeve pipe (803), clean hole (806) have been seted up to the outside equidistance of sleeve pipe (803).

6. The fluorescence quantitative PCR detection kit for the enterobacter hepatica in water according to claim 5, wherein: the top end of the sleeve (803) is movably sleeved with the bottom end of the water outlet pipe (703), and the adjacent gears (804) are not contacted.

7. The fluorescence quantitative PCR detection kit for the enterobacter hepatica in water according to claim 5, wherein: the cleaning contact pins (805) and the cleaning holes (806) are arranged on the outer side of the sleeve (803) at intervals in a crossed mode, and the outer ends of the cleaning contact pins (805) are in contact with the inner wall of the test tube (5).