CN216094306U

CN216094306U - Cell culture bottle centrifugation liquid level assists balancing unit

Info

Publication number: CN216094306U
Application number: CN202122902985.5U
Authority: CN
Inventors: 陶晓莉; 马巍
Original assignee: Jinzhou Medical University
Current assignee: Jinzhou Medical University
Priority date: 2021-11-23
Filing date: 2021-11-23
Publication date: 2022-03-22
Anticipated expiration: 2031-11-23

Abstract

The utility model discloses a cell culture bottle centrifugal liquid level auxiliary balancing device which comprises a balancing seat and a bottom plate, wherein a placing groove is formed in the top of the balancing seat, a culture bottle is arranged in the placing groove, fixing plates are fixedly arranged below two sides of the balancing seat, internal thread sleeves are fixedly arranged in four fixing plates, screw rods are arranged in the four fixing plates through the internal thread sleeves, and connecting seats are fixedly arranged in the middle positions of the tops of the four bottom plates. According to the utility model, the knobs on two sides of the balancing seat are rotated to drive the screw rod to rotate, so that the screw rod can move up and down under the action of the internal thread sleeve, the position heights of four ends of the balancing seat can be adjusted, the liquid level layer of the culture solution can be parallel to the bottom surface of the cell culture bottle through continuous adjustment, and the liquid level of the cell culture bottle is uniformly distributed.

Description

Cell culture bottle centrifugation liquid level assists balancing unit

Technical Field

The utility model relates to the technical field of centrifuges, in particular to an auxiliary balancing device for a centrifugal liquid level of a cell culture bottle.

Background

Traditional centrifuge mainly centrifuges containers such as centrifugation heparin tube, centrifuging tube, and the bottom of centrifuging tube is circular or toper mostly, and is not high to the balancing requirement, and cell compressive stress centrifuge then requires to place the cell culture bottle in the centrifuge centrifugation, and the atress bottom is a plane, and is extremely high to the balancing requirement, requires that the even cover of thin one deck culture solution is on the cell of cultivateing in centrifugal process.

At present, when a cell culture bottle is centrifuged, if the cell culture bottle is not well balanced, the culture solution can be deviated under the action of centrifugal force and gravity, part of cells are not covered by the culture solution, the cells die after centrifugation, and the cells are also subjected to other interference stress effects except for compressive stress, so that the accuracy of an experimental result is influenced. Therefore, in order to solve the problems, the centrifugal liquid level auxiliary balancing device for the cell culture bottle is improved.

SUMMERY OF THE UTILITY MODEL

In order to solve the technical problems, the utility model provides the following technical scheme:

the utility model provides a balancing device is assisted to cell culture bottle centrifugation liquid level, includes balancing seat and bottom plate, the standing groove has been seted up at the top of balancing seat, the inside of standing groove is provided with the blake bottle, the equal fixed mounting in below of balancing seat both sides has a fixed plate, four the equal fixed mounting in inside of fixed plate has the internal thread cover, four the fixed plate is all through inside the lead screw is installed to the internal thread cover, four the equal fixed mounting in intermediate position department at bottom plate top has the connecting seat.

As a preferable technical solution of the present invention, two telescopic rods are fixedly installed at one side inside the placement groove, and a triangular block is fixedly installed at one end of each of the two telescopic rods.

As a preferable technical scheme of the utility model, the outer sides of the two telescopic rods are sleeved with second springs, and the inclined plane at one side of the triangular block is provided with a soft pad.

As a preferred technical scheme, movable balls are arranged inside the four connecting seats, the bottom ends of the four screw rods are respectively connected with the surfaces of the four movable balls, and knobs are fixedly mounted at the top ends of the four screw rods.

As a preferred technical scheme of the utility model, bubble levels are fixedly installed at the middle position of the left side of the top of the balancing seat and the middle position of the rear side of the top, an anti-slip mat is arranged at the bottom of the inner side of the placing groove, and anti-slip lines are arranged on the upper surface of the anti-slip mat.

As a preferred technical scheme, the bottom of the leveling seat is provided with two mounting grooves, the tops of the inner sides of the two mounting grooves are fixedly provided with a plurality of first springs, the mounting plates are mounted on the two mounting grooves through the plurality of first springs, the tops of the two mounting plates are fixedly provided with fixing rings, and the surfaces of the inner sides of the two fixing rings are provided with protection pads.

The utility model has the beneficial effects that:

through observing the position of bubble in the bubble spirit level, rotate the knob of balancing seat both sides, the knob will drive the lead screw rotatory for can up-and-down motion under the effect of internal thread cover of lead screw, thereby can adjust the position height of balancing seat four ends, through constantly adjusting, can make the liquid level layer of culture solution parallel with cell culture bottle bottom surface, and make cell culture bottle's liquid level evenly distributed.

Drawings

The accompanying drawings, which are included to provide a further understanding of the utility model and are incorporated in and constitute a part of this specification, illustrate embodiments of the utility model and together with the description serve to explain the principles of the utility model and not to limit the utility model. In the drawings:

FIG. 1 is a schematic structural view of a centrifugal liquid level auxiliary balancing device of a cell culture flask according to the utility model;

FIG. 2 is a top view of a cell culture flask centrifuge level assisted balancing apparatus of the present invention;

FIG. 3 is an enlarged schematic view of the cell culture flask centrifugal liquid level auxiliary balancing device at A in FIG. 1;

in the figure: 1. leveling seats; 2. a culture bottle; 3. a non-slip mat; 4. mounting a plate; 5. a first spring; 6. mounting grooves; 7. a triangular block; 8. a telescopic rod; 9. a second spring; 10. a fixing ring; 11. a bubble level; 12. a knob; 13. a fixing plate; 14. a base plate; 15. a placement groove; 16. a connecting seat; 17. a movable ball; 18. a screw rod; 19. an internal thread sleeve; 20. a protective pad.

Detailed Description

The preferred embodiments of the present invention will be described in conjunction with the accompanying drawings, and it will be understood that they are described herein for the purpose of illustration and explanation and not limitation.

Referring to fig. 1-3, the present invention provides a technical solution: the utility model provides a cell culture bottle centrifugation liquid level assists balancing device technical scheme, including balancing seat 1 and bottom plate 14, the standing groove 15 has been seted up at the top of balancing seat 1, the inside of standing groove 15 is provided with blake bottle 2, the equal fixed mounting in below of balancing seat 1 both sides has fixed plate 13, the equal fixed mounting in inside of four fixed plates 13 has internal thread cover 19, four fixed plates 13 all install lead screw 18 through inside internal thread cover 19, the equal fixed mounting in intermediate position department at four bottom plate 14 tops has connecting seat 16.

The fixed mounting of the inside one side of standing groove 15 has two telescopic links 8, and the one end fixed mounting of two telescopic links 8 has three hornblocks 7, through three hornblocks 7 that set up, can support the inclined plane of 2 one sides of blake bottle for 2 places the back more stable.

The outside of two telescopic links 8 all is equipped with second spring 9, and the inclined plane of three hornblocks 7 one side is provided with the cushion, through the second spring 9 that sets up, can make three hornblocks 7 coincide with the inclined plane of blake bottle 2 one side all the time.

The inside of four connecting seats 16 all is provided with movable ball 17, and the bottom of four lead screws 18 is connected with four movable ball 17's surface respectively, and the equal fixed mounting in top of four lead screws 18 has knob 12, through the movable ball 17 that sets up, can make lead screw 18 can incline to multiple direction, through rotatory knob 12, can drive lead screw 18 rotatory for lead screw 18 can up-and-down motion under the effect of internal thread cover 19.

The equal fixed mounting in intermediate position department of the left intermediate position department in balance seat 1 top and the intermediate position department of top rear side has bubble spirit level 11, and the bottom of standing groove 15 inboard is provided with slipmat 3, and the upper surface of slipmat 3 is provided with anti-skidding line, through observing the position of bubble in the horizontal bubble appearance 11, can know whether balance seat 1 is in the horizontality, is convenient for carry out the balance to the culture solution in blake bottle 2.

Two mounting grooves 6 have been seted up to the bottom of joining in marriage flat seat 1, the equal fixed mounting in top of 6 inboards in two mounting grooves has a plurality of first springs 5, mounting panel 4 is all installed through a plurality of first springs 5 of inboard in two mounting panel 6, the equal fixed mounting in top of two mounting panels 4 has solid fixed ring 10, the inboard surface of two solid fixed ring 10 all is provided with the protection pad 20, first spring 5 can give mounting panel 4 certain elasticity, drive mounting panel 4 downstream, make solid fixed ring 10 fix blake bottle 2 in the inside of mounting groove 6.

The working principle is as follows: before use, the safety of each structure of the utility model is checked, when the cell culture bottle centrifugal liquid level auxiliary balancing device is used, the fixing ring 10 is lifted at first, the culture bottle 2 is placed in the placing groove 6, at the moment, the fixing ring 10 is loosened, the first spring 5 gives a certain elastic force to the mounting plate 4, the mounting plate 4 is driven to move downwards, the culture bottle 2 is fixed in the mounting groove 6 by the fixing ring 10, and the culture bottle 2 is prevented from being separated from the balancing seat 1 during centrifugation.

After the culture bottle 1 is fixed, the knobs 12 on two sides of the balancing seat 1 are rotated by observing the position of bubbles in the bubble level meter 11, the knobs 12 drive the screw rod 18 to rotate, so that the screw rod 18 can move up and down under the action of the internal thread sleeve 19, the height of the positions of four ends of the balancing seat 1 can be adjusted, the liquid level layer of the culture solution is parallel to the bottom surface of the cell culture bottle, and the liquid level of the cell culture bottle is uniformly distributed.

In the description of the present invention, it should be noted that the terms "vertical", "upper", "lower", "horizontal", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of describing the present invention and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.

In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the utility model. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. The utility model provides a supplementary balancing unit of cell culture bottle centrifugation liquid level, includes balancing seat (1) and bottom plate (14), its characterized in that, standing groove (15) have been seted up at the top of balancing seat (1), the inside of standing groove (15) is provided with blake bottle (2), the equal fixed mounting in below of balancing seat (1) both sides has fixed plate (13), four the equal fixed mounting in inside of fixed plate (13) has internal thread cover (19), four fixed plate (13) all through inside lead screw (18) are installed to internal thread cover (19), four equal fixed mounting in intermediate position department at bottom plate (14) top has connecting seat (16).

2. The cell culture bottle centrifugal liquid level auxiliary balancing device according to claim 1, characterized in that two telescopic rods (8) are fixedly installed on one side inside the placing groove (15), and a triangular block (7) is fixedly installed at one end of each telescopic rod (8).

3. The cell culture bottle centrifugal liquid level auxiliary balancing device as claimed in claim 2, wherein the outer sides of the two telescopic rods (8) are sleeved with second springs (9), and the inclined surface of one side of the triangular block (7) is provided with a soft cushion.

4. The cell culture bottle centrifugal liquid level auxiliary balancing device as claimed in claim 1, wherein the four connecting seats (16) are provided with movable balls (17) inside, the bottom ends of the four lead screws (18) are respectively connected with the surfaces of the four movable balls (17), and the top ends of the four lead screws (18) are fixedly provided with knobs (12).

5. The cell culture bottle centrifugal liquid level auxiliary balancing device according to claim 1, wherein the bubble level meter (11) is fixedly installed at the middle position of the left side of the top of the balancing seat (1) and the middle position of the rear side of the top, the bottom of the inner side of the placing groove (15) is provided with the non-slip mat (3), and the upper surface of the non-slip mat (3) is provided with non-slip lines.

6. The cell culture bottle centrifugal liquid level auxiliary balancing device according to claim 1, wherein two mounting grooves (6) are formed in the bottom of the balancing seat (1), a plurality of first springs (5) are fixedly mounted on the tops of the inner sides of the two mounting grooves (6), mounting plates (4) are mounted on the mounting grooves (6) through the plurality of first springs (5) on the inner sides, fixing rings (10) are fixedly mounted on the tops of the two mounting plates (4), and protective pads (20) are arranged on the surfaces of the inner sides of the two fixing rings (10).