CN216082515U

CN216082515U - Microscopic observation device

Info

Publication number: CN216082515U
Application number: CN202122536401.7U
Authority: CN
Inventors: 张冀聪; 李翔; 王海波; 王�华; 曹朝晖
Original assignee: Hefei Innovation Research Institute of Beihang University
Current assignee: Hefei Innovation Research Institute of Beihang University
Priority date: 2021-10-21
Filing date: 2021-10-21
Publication date: 2022-03-18
Anticipated expiration: 2031-10-21

Abstract

The utility model relates to the technical field of microscopic observation equipment, and discloses a microscopic observation device. It includes incubator, sample microscope carrier, light source subassembly and collection subassembly. The sample carrying platform is arranged in the incubator and is used for placing a sample to be observed, and an observation window is arranged on the wall of the incubator on one side of the sample carrying platform; the light source assembly is arranged in the incubator and positioned on the other side of the sample carrying platform, and comprises light sources and first reflectors which are arranged at intervals along a first direction, and the first direction is parallel to the sample carrying platform; the collecting assembly is arranged outside the incubator and comprises an objective lens, a second reflector and a camera, the objective lens is arranged right opposite to the observation window, the second reflector is arranged on a light path between the objective lens and the camera, and the second reflector can change the angle of the light path; the light path emitted by the light source sequentially passes through the first reflector, the observation window, the objective lens and the second reflector and reaches the camera. The utility model can shorten the length of the light source component, reduce the volume of the incubator and is beneficial to the miniaturization of the microscopic observation device.

Description

Microscopic observation device

Technical Field

The utility model relates to the technical field of microscopic observation equipment, in particular to a microscopic observation device.

Background

The increase of the living pressure of modern people and the aggravation of environmental pollution, the incidence rate of infertility of women is continuously increased. For this reason, assisted reproduction technology derived from in vitro fertilization-embryo transfer (IVF) has become a research hotspot in this field. In vitro culture of embryonic cells is typically observed using an embryo viewing culture device. At present, embryo inspection culture apparatus with a plurality of independent cavities is widely used, and an independent temperature regulation system and a light source assembly are arranged in the cavity of each incubator. The light source assembly is arranged above the sample carrying platform and comprises a lens barrel and a light source, the axis of the lens barrel is perpendicular to the sample carrying platform, the light source is arranged at one end of the lens barrel, and the other end of the lens barrel faces the sample carrying platform. However, the light source assembly in the existing incubator is long in length, so that the height of the incubator is large, and the incubator is not favorable for observing the miniaturization of the culture device.

Based on this, a microscope observation device is needed to solve the above problems.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a microscopic observation device, which is used for shortening the length of a light source component, thereby reducing the volume of an incubator and being beneficial to the miniaturization of the microscopic observation device.

In order to achieve the purpose, the utility model adopts the following technical scheme:

a microscopic viewing apparatus, comprising:

an incubator;

the sample carrying platform is arranged in the incubator and used for placing a sample to be observed, and an observation window is arranged on the wall of the incubator on one side of the sample carrying platform;

the light source assembly is arranged in the incubator and positioned on the other side of the sample carrying platform, and comprises light sources and first reflectors which are arranged at intervals along a first direction, and the first direction is parallel to the sample carrying platform;

the collection assembly is arranged outside the incubator and comprises an objective lens, a second reflector and a camera, the objective lens is arranged right opposite to the observation window, the second reflector is arranged on a light path between the objective lens and the camera, and the second reflector can change the angle of the light path;

and the light path emitted by the light source sequentially passes through the first reflector, the observation window, the objective lens and the second reflector and reaches the camera.

Optionally, the light source assembly further includes a lens barrel, the light source and the first reflector are both disposed in the lens barrel, the lens barrel includes a first barrel portion and a second barrel portion that are connected in an L shape, the first barrel portion is disposed parallel to the sample stage, the second barrel portion is disposed perpendicular to the sample stage and extends toward the sample stage, the light source is disposed at one end of the first barrel portion that is far away from the second barrel portion, and the first reflector is disposed at a connection position between the first barrel portion and the second barrel portion.

Optionally, the light source assembly further includes a light-homogenizing mirror, the light-homogenizing mirror is disposed in the first cylinder, and the light-homogenizing mirror is located between the light source and the first reflector, and the light-homogenizing mirror can homogenize light emitted by the light source.

Optionally, the dodging mirror is a double-row fly-eye lens array.

Optionally, the light source assembly further comprises a collecting mirror, the collecting mirror is arranged at one end, far away from the first barrel, of the second barrel, and the collecting mirror can collect light from the first reflecting mirror.

Optionally, the condenser is a super-surface lens, the super-surface lens includes a substrate disposed parallel to the sample stage, one side of the substrate facing the first reflector is connected with a plurality of convex pillars, and the plurality of convex pillars are arranged in an array.

Optionally, the collection assembly further comprises a connector disposed parallel to the sample stage, the second mirror and the camera both being disposed on the connector.

Optionally, the objective lens is a huffman objective lens.

Optionally, the sample stage is electrically heated glass.

Optionally, the incubator comprises a case body and a cover body, one end of the case body is open, the cover body covers the opening, and the light source assembly is connected to the cover body.

The utility model has the beneficial effects that: the microscopic observation device provided by the utility model comprises an incubator, a sample carrier, a light source assembly and a collection assembly. Set up sample microscope carrier and light source subassembly in the incubator, the image of sample of waiting to observe is gathered through the observation window on the incubator to the collection subassembly. The light source subassembly includes first speculum, and first speculum sets up along the first direction interval that is on a parallel with the sample microscope carrier with the light source, can reduce the length size of light source subassembly, does benefit to and reduces the incubator along the size of perpendicular to sample microscope carrier direction for the inside structure of incubator is compacter, does benefit to incubator and microscopic observation device's miniaturization.

Drawings

FIG. 1 is a schematic structural diagram of a microscopic observation device provided in an embodiment of the present invention;

fig. 2 is a schematic structural diagram of a super-surface lens provided in an embodiment of the present invention.

In the figure:

10. a sample to be observed;

1. an incubator; 11. a box body; 12. a box cover;

2. a sample stage;

3. a light source assembly; 31. a light source; 32. a first reflector; 33. a light homogenizing mirror; 34. a condenser lens; 341. a substrate; 342. a convex column; 35. a lens barrel; 351. a first tube section; 352. a second cylinder part; 36. a polarizer; 37. an analyzer; 38. a rectangular diaphragm;

4. a collection assembly; 41. an objective lens; 42. a second reflector; 43. a camera; 44. a connecting member; 45. placing a box; 46. a cylindrical mirror;

5. a culture dish.

Detailed Description

In order to make the technical problems solved, technical solutions adopted and technical effects achieved by the present invention clearer, the technical solutions of the embodiments of the present invention will be described in further detail below with reference to the accompanying drawings, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, unless expressly stated or limited otherwise, the terms "connected," "connected," and "fixed" are to be construed broadly, e.g., as meaning permanently connected, removably connected, or integral to one another; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

In the present invention, unless otherwise expressly stated or limited, "above" or "below" a first feature means that the first and second features are in direct contact, or that the first and second features are not in direct contact but are in contact with each other via another feature therebetween. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.

The technical scheme of the utility model is further explained by the specific implementation mode in combination with the attached drawings.

The embodiment provides a microscopic observation device. Specifically, as shown in fig. 1, the microscopic observation apparatus includes: incubator 1, sample microscope carrier 2, light source subassembly 3 and collection subassembly 4. The sample carrying platform 2 is arranged in the incubator 1 and is used for placing a sample 10 to be observed, and an observation window is arranged on the wall of the incubator 1 at one side of the sample carrying platform 2; the light source assembly 3 is arranged in the incubator 1 and is positioned at the other side of the sample carrier 2, the light source assembly 3 comprises a light source 31 and a first reflector 32 which are arranged at intervals along a first direction, and the first direction is parallel to the sample carrier 2; the collection component 4 is arranged outside the incubator 1 and comprises an objective 41, a second reflector 42 and a camera 43, the objective 41 is arranged right opposite to the observation window, the second reflector 42 is arranged on the light path between the objective 41 and the camera 43, and the angle of the light path can be changed by the second reflector 42; the light path from the light source 31 passes through the first mirror 32, the observation window, the objective lens 41, the second mirror 42, and reaches the camera 43 in this order.

The microscopic observation device provided by the embodiment comprises an incubator 1, a sample carrier 2, a light source assembly 3 and a collection assembly 4. Set up sample microscope carrier 2 and light source subassembly 3 in the incubator 1, gather the image that subassembly 4 was through the observation window collection on the incubator 1 waits to observe sample 10. Light source subassembly 3 includes first speculum 32, and first speculum 32 sets up along the first direction interval that is on a parallel with sample microscope carrier 2 with light source 31, can reduce light source subassembly 3's length size, does benefit to and reduces incubator 1 along the size of perpendicular to sample microscope carrier 2 direction for the inside structure of incubator 1 is compacter, does benefit to incubator 1 and microscopic observation device's miniaturization.

In this embodiment, light source subassembly 3 arranges the top in incubator 1 in, and sample microscope carrier 2 level sets up and is located the bottom in incubator 1, and the observation window has been seted up to the bottom surface of incubator 1, and observation window department is provided with glass, can maintain the stable environment in incubator 1, also is convenient for observe and treats observation sample 10.

Further, set up culture dish 5 on sample microscope carrier 2, wait to observe sample 10 and arrange in culture dish 5, the better organic polymer of biocompatibility is selected for use to the material of culture dish 5, like polystyrene, and its luminousness is greater than 90%, is convenient for observe and waits to observe sample 10.

Further, sample microscope carrier 2 is electrical heating glass, and sample microscope carrier 2 arranges in the bottom surface of incubator 1 on, treats that observation sample 10 arranges in sample microscope carrier 2 through culture dish 5 on, and electrical heating glass can heat and treat observation sample 10, also can maintain the inside temperature of incubator 1, guarantees to treat the cultivation environment of observing sample 10, and electrical heating glass is transparent material in addition, is convenient for observe and treats observation sample 10, has also guaranteed the quality of formation of image collection. The structure of the electrically heated glass can be made by the prior art, and is not described in detail herein.

Preferably, the incubator 1 includes a case 11 and a cover 12, wherein one end of the case 11 is open, specifically, the top end of the case 11 is open, the cover 12 covers the opening, and the light source assembly 3 is connected to the cover 12. The tank cover 12 is provided to facilitate installation and maintenance of the structure inside the incubator 1. Lid 12 and box 11 pass through the hinge rotation and are connected, and lid 12 and box 11 are preferably sealed setting, do benefit to the temperature and the humidity of maintaining in incubator 1, provide stable cultivation environment.

Further, still be equipped with drive assembly in the incubator 1, can drive sample microscope carrier 2 and remove along the horizontal direction, be convenient for observe a plurality of samples 10 of waiting to observe on the culture dish 5 through the observation window.

Preferably, light source assembly 3 further includes lens barrel 35, light source 31 and first reflecting mirror 32 are disposed in lens barrel 35, lens barrel 35 includes first cylindrical portion 351 and second cylindrical portion 352 connected in an L-shape, first cylindrical portion 351 is disposed parallel to sample stage 2, second cylindrical portion 352 is disposed perpendicular to sample stage 2 and extends toward sample stage 2, light source 31 is disposed at one end of first cylindrical portion 351 away from second cylindrical portion 352, and first reflecting mirror 32 is disposed at a connection between first cylindrical portion 351 and second cylindrical portion 352. Set up lens cone 35, the inside structure of protection light source subassembly 3 also does benefit to the structure modularization of light source subassembly 3, the dismouting of being convenient for. Specifically, the first tube portion 351 and the second tube portion 352 have the same diameter and are integrally provided, and the first tube portion 351 is fixedly connected to the lid body 12 by bonding or bolting. Specifically, the light source 31 employs a light source of red color and wavelength of 635 nm.

Further, the light source assembly 3 further includes a light-homogenizing mirror 33, the light-homogenizing mirror 33 is disposed in the first barrel 351, the light-homogenizing mirror 33 is located between the light source 31 and the first reflector 32, and the light-homogenizing mirror 33 can homogenize the light emitted by the light source 31, so that the brightness of the illumination to the sample 10 to be observed is more uniform, the observation effect is ensured, and the definition of the collected image is also ensured.

In the embodiment, the light uniformizing lens 33 is a double-row fly-eye lens array, and the specific structure and the homogenization principle thereof are the prior art and are not described herein again. The double-row fly-eye lens array comprises two rows of fly-eye lenses arranged in parallel. The fly-eye lens array on the side facing the light source 31 is a first row of fly-eye lens array, and the fly-eye lens array on the side away from the light source 31 is a second row of fly-eye lens array. The focal point of each unit lens in the first row of fly-eye lens array coincides with the center of the corresponding unit lens in the second row of fly-eye lens array, and the optical axes of the two rows of fly-eye lens arrays are parallel to each other.

Further, the light source assembly 3 further includes a collecting lens 34, the collecting lens 34 is disposed at one end of the second cylinder portion 352 far away from the first cylinder portion 351, the collecting lens 34 can collect light from the first reflector 32, so that light can be collected on the sample 10 to be observed on the sample stage 2, and the definition of the image is ensured.

As shown in fig. 1-2, the condenser lens 34 is a super-surface lens, which has a condensing function. The super-surface lens includes a substrate 341 disposed parallel to the sample stage 2, a plurality of convex pillars 342 are connected to one side of the substrate 341 facing the first reflecting mirror 32, and the plurality of convex pillars 342 are arranged in an array. Compared with the traditional convex lens, the super-surface lens is thinner, the super-surface lens is used for completing the light converging effect, the length of the second cylinder part 352 is favorably shortened, the miniaturization of the incubator 1 in the height direction is further favorably realized, and the miniaturization of the whole microscopic observation device is favorably realized.

In the present embodiment, the pillar 342 has a cylindrical structure, the substrate 341 has a disc-shaped structure, and both the substrate 341 and the pillar 342 are made of a transparent material, preferably the same material, such as silicon dioxide. Further, the protruding column 342 and the base 341 are integrated, so as to facilitate processing. The principle of converging light by the super-surface lens is the prior art and is not described in detail herein. In other embodiments, a common convex lens or a super-surface lens with other structures may be used to achieve the light-gathering effect, which is not limited herein.

In this embodiment, the phase profile of the super-surface lens is a hyperboloid type, which utilizes the phase jump of the sub-wavelength scatterer to achieve the convergence function. The phase distribution of the super-surface lens is as follows:

wherein r is the coordinate position of the super-surface lens unit structure (the convex column 342), and f is the focal length of the super-surface lens. Preferably, the numerical aperture of the super-surface lens is 0.8.

Preferably, the light source assembly 3 further comprises a polarizer 36, an analyzer 37 and a rectangular diaphragm 38. Specifically, the polarizer 36 is located between the analyzer 37 and the light source 31, specifically between the first reflecting mirror 32 and the dodging mirror 33, and the light beam emitted from the light source 31 forms linearly polarized light when passing through the polarizer 36. The rectangular diaphragm 38 is arranged on the circular light shielding plate, and the rectangular diaphragm 38 is positioned on one side close to the edge of the light shielding plate, so that oblique illumination can be provided, the improvement of the imaging resolution ratio is facilitated, and the formation of a three-dimensional image is facilitated. The area of the rectangular aperture 38 on the side remote from the edge is provided with half the total light pass and half the light pass of the plated polarizing film (i.e. forming the analyzer 37). The condenser lens 34 is located below the rectangular diaphragm 38 for converging light rays onto the sample 10 to be observed, and the rectangular diaphragm 38 is located at the front focal plane of the condenser lens 34. In this embodiment, the polarizer 36, the analyzer 37, the rectangular diaphragm 38 and the condenser 34 form a condenser structure of the hoffman microscope, and the operation principle and structure thereof are not further described herein.

Preferably, the collecting assembly 4 includes a placing box 45, the second reflecting mirror 42 is disposed in the placing box 45, the objective lens 41 is disposed on the top surface of the placing box 45, a through hole is formed on the top surface of the placing box 45, and the optical path between the objective lens 41 and the second reflecting mirror 42 can pass through the through hole. Specifically, the objective lens 41 is located directly below the observation window. Set up the through-hole on placing the left side lateral wall of case 45, camera 43 arranges the left side of placing case 45 in, and camera 43 and place and be provided with a section of thick bamboo mirror 46 between the left through-hole of case 45, adopt C type mouth to be connected between section of thick bamboo mirror 46 and the camera 43. The cylindrical mirror 46 and the C-shaped opening can be implemented by the prior art, but are not limited thereto. The camera 43 is a CCD or CMOS camera, and the field of view of the microscopic imaging is 200 μm, and the resolution is 3 pixels/μm.

Preferably, acquisition assembly 4 further comprises a connector 44, connector 44 being arranged parallel to sample stage 2, and second mirror 42 and camera 43 being arranged on connector 44. The modularization degree of the acquisition assembly 4 is improved, and the assembly and disassembly are convenient.

In the present embodiment, the connector 44 has a plate-like structure, and can carry the camera 43 and the second reflecting mirror 42 so as to be spaced apart from each other in a direction parallel to the sample stage 2, thereby further reducing the dimension of the incubator 1 in the height direction,

in this embodiment, the objective lens 41 is a hoffman objective lens, and the observation effect is good. Preferably, the Hofmann objective has a magnification of 20X and a working distance of 2.5 mm. An amplitude modulator is arranged on one side, away from the incubator 1, of the Hoffman objective lens and is divided into three areas with light transmittance of 1%, 15% and 100%, so that the embryo picture can generate a relief effect. The structure of the hoffman objective lens is the prior art, and is not described herein again.

In other embodiments, the objective lens 41 may be other types of objective lenses, and has a magnifying effect, and is not limited herein.

It should be understood that the above-described embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.

Claims

1. A microscopic viewing apparatus, comprising:

an incubator (1);

the sample carrying platform (2) is arranged in the incubator (1) and is used for placing a sample (10) to be observed, and an observation window is arranged on the wall of the incubator (1) on one side of the sample carrying platform (2);

the light source assembly (3) is arranged in the incubator (1) and is positioned on the other side of the sample carrying platform (2), the light source assembly (3) comprises light sources (31) and a first reflector (32) which are arranged at intervals along a first direction, and the first direction is parallel to the sample carrying platform (2);

the collection assembly (4) is arranged outside the incubator (1) and comprises an objective lens (41), a second reflector (42) and a camera (43), the objective lens (41) is arranged right opposite to the observation window, the second reflector (42) is arranged on a light path between the objective lens (41) and the camera (43), and the angle of the light path can be changed by the second reflector (42);

the light path emitted by the light source (31) sequentially passes through the first reflector (32), the observation window, the objective lens (41) and the second reflector (42) and reaches the camera (43).

2. The microscopic observation apparatus according to claim 1, wherein the light source assembly (3) further comprises a lens barrel (35), the light source (31) and the first reflecting mirror (32) are both disposed in the lens barrel (35), the lens barrel (35) comprises a first barrel portion (351) and a second barrel portion (352) connected in an L-shape, the first barrel portion (351) is disposed parallel to the sample stage (2), the second barrel portion (352) is disposed perpendicular to the sample stage (2) and extends toward the sample stage (2), the light source (31) is disposed at one end of the first barrel portion (351) away from the second barrel portion (352), and the first reflecting mirror (32) is disposed at a connection between the first barrel portion (351) and the second barrel portion (352).

3. Microscopic observation apparatus according to claim 2, wherein the light source assembly (3) further comprises a light homogenizing mirror (33), the light homogenizing mirror (33) being disposed within the first barrel (351), and the light homogenizing mirror (33) being located between the light source (31) and the first reflector (32), the light homogenizing mirror (33) being capable of homogenizing the light emitted by the light source (31).

4. Microscopic observation apparatus according to claim 3, wherein said dodging mirror (33) is a double row fly's eye lens array.

5. Microscopic observation apparatus according to claim 2, wherein the light source assembly (3) further comprises a condenser lens (34), the condenser lens (34) being disposed at an end of the second barrel (352) remote from the first barrel (351), the condenser lens (34) being capable of condensing light from the first reflector (32).

6. Microscopic observation apparatus according to claim 5, wherein the condenser (34) is a super surface lens, the super surface lens comprises a base (341) arranged parallel to the sample stage (2), a plurality of convex columns (342) are connected to a side of the base (341) facing the first reflector (32), and the plurality of convex columns (342) are arranged in an array.

7. Microscopic observation apparatus according to claim 1, characterized in that the acquisition assembly (4) further comprises a connector (44), the connector (44) being arranged parallel to the sample stage (2), the second mirror (42) and the camera (43) being placed on the connector (44).

8. Microscopic observation apparatus according to claim 1, characterized in that the objective (41) is a huffman objective.

9. Microscopic observation apparatus according to claim 1, characterized in that the sample stage (2) is an electrically heated glass.

10. The microscopic observation apparatus according to claim 1, wherein the incubator (1) comprises a case (11) and a cover (12), one end of the case (11) is open, the cover (12) is disposed on the opening, and the light source assembly (3) is connected to the cover (12).