CN210323559U - Microsphere-based transmission type high-resolution microscopic imaging system - Google Patents
Microsphere-based transmission type high-resolution microscopic imaging system Download PDFInfo
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- CN210323559U CN210323559U CN201921305213.XU CN201921305213U CN210323559U CN 210323559 U CN210323559 U CN 210323559U CN 201921305213 U CN201921305213 U CN 201921305213U CN 210323559 U CN210323559 U CN 210323559U
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Abstract
The utility model discloses a transmission-type high-resolution microscopic imaging system based on microspheres, which comprises microspheres, a microscopic camera module, a receiving terminal, a sample and an excitation light source, wherein the microspheres are placed on the upper surface of the sample, the excitation light source is positioned below the sample, the microspheres are covered with a cover glass, and the microscopic camera module is positioned right above the excitation light source; the microscopic camera module comprises a first lens group, a second lens group and an image sensor, wherein the first lens group and the second lens group are symmetrically arranged, the first lens group is located between the second lens group and the cover glass, the second lens group is located between the first lens group and the image sensor, and the image sensor is electrically connected with the receiving end. The utility model has the advantages of resolution ratio is high, small, simple structure, with low costs, stability is high, modularization, be applicable to the test transparent sample.
Description
Technical Field
The utility model belongs to the technical field of microscopic imaging, especially, relate to a transmission-type high-resolution microscopic imaging system based on microballon.
Background
The microscopic imaging system is an optical system or an instrument which can magnify and image tiny objects or details which are difficult to observe or distinguish by human eyes so as to extract fine structure information, and related products are widely used in the fields related to the aspects of experimental research, production and manufacture and the like. As related disciplines progress in the microscopic field, microscopic imaging systems based on many leading-edge theories have broken through the optical imaging limits, moving towards higher resolutions.
At present, although a high-resolution microscope on the market, such as a fluorescence microscope, can achieve excellent microscopic resolution, the whole imaging system is complex and bulky, has no portability, and is expensive, and in addition, due to the precise structure, the imaging effect is easily interfered by external factors.
In summary, the conventional high-resolution microscope is generally large in size, high in cost and poor in environmental adaptability.
In conclusion, the micro-imaging system with the advantages of large view field, high resolution, strong stability, small volume and low cost is realized, and the micro-imaging system has a very high practical value.
SUMMERY OF THE UTILITY MODEL
The utility model discloses the main technical problem who solves provides a micro-imaging system of transmission-type high-resolution based on microballon, has advantages such as resolution ratio height, small, simple structure, with low costs, stability height, modularization, is applicable to the transparent sample of test.
In order to solve the technical problem, the utility model discloses a technical scheme be: a transmission-type high-resolution microscopic imaging system based on microspheres comprises the microspheres, a microscopic camera module, a receiving end, a sample and an excitation light source, wherein the microspheres are placed on the upper surface of the sample, the excitation light source is positioned below the sample, a cover glass is covered on the microspheres, and the microscopic camera module is positioned right above the excitation light source;
the microscopic camera module comprises a first lens group, a second lens group and an image sensor, wherein the first lens group and the second lens group are symmetrically arranged, the first lens group is located between the second lens group and the cover glass, the second lens group is located between the first lens group and the image sensor, and the image sensor is electrically connected with the receiving end.
The utility model discloses a solve the further technical scheme that its technical problem adopted and be:
further, the second lens group includes at least two microlenses, a size of the microlens near the image sensor is largest, and a size of the microlens far from the image sensor is smallest; the first lens group comprises at least two micro lenses, the size of the micro lens close to the image sensor is the smallest, and the size of the micro lens far away from the image sensor is the largest; the size of the micro lens is 0.5-15 mm in diameter.
Further, the microspheres are transparent microspheres, and the diameter of the microspheres is 5-300 microns.
Further, the microspheres are barium titanate microspheres or polystyrene microspheres.
Further, the microlenses with the smallest diameter of the first lens group and the microlenses with the smallest diameter of the second lens group are different microlenses or the same microlens.
Further, a focal plane of the second lens group coincides with a receiving end face of the image sensor, a focal length of the second lens group is 1-3mm, and an F number of the second lens group is less than 3.
Further, the focal length of the first lens group is 1-8mm, and the F number of the first lens group is less than 3.
Further, the image element size of the image sensor is 0.8-2.5 μm.
Further, the image sensor is a CMOS image sensor or a CCD image sensor.
Further, the excitation light source is a laser light source, an LED light source or a gas light source.
The utility model has the advantages that:
all parts of the whole system are of modular structures, so that the system is convenient to replace or combine with each other, and is strong in overall stability and not easy to deform; the magnification and the field of view of the microscopic imaging can be adjusted by adjusting the ratio of the focal lengths of the first lens group and the second lens group. Moreover, the system has small integral volume, regular shape and strong portability, and is easy to be modified according to actual needs, such as forming a micro-camera array and the like;
in addition, in the optical path, the microspheres can play a role in amplifying the ball lens, and on the other hand, the microspheres tightly attached to the sample can effectively couple evanescent waves on the surface of the sample into the optical path due to small size and contact with the surface of the sample, and the evanescent waves contain a large amount of detailed information on the surface of the sample, so that the imaging resolution can be effectively improved by the method, and the resolution of the system can reach below 0.5 micron;
in addition, the system is portable as a whole, and can be directly placed on the surface of a sample to perform real-time in-situ imaging monitoring.
Drawings
Fig. 1 is a schematic structural diagram of embodiment 1 of the present invention (the microlens with the smallest diameter of the first lens group is different from the microlens with the smallest diameter of the second lens group);
fig. 2 is a schematic structural diagram of embodiment 2 of the present invention (the microlens having the smallest diameter of the first lens group is the same as the microlens having the smallest diameter of the second lens group);
the parts in the drawings are marked as follows:
the device comprises a microsphere 1, a microscopic camera module 2, a first lens group 21, a second lens group 22, an image sensor 23, a receiving end 3, a sample 4, an excitation light source 5 and a cover glass 6.
Detailed Description
The following detailed description of the preferred embodiments of the present invention will be provided in conjunction with the accompanying drawings, so as to enable those skilled in the art to more easily understand the advantages and features of the present invention, and thereby define the scope of the invention more clearly and clearly.
Example 1: a microsphere-based transmission-type high-resolution microscopic imaging system, as shown in fig. 1, includes a microsphere 1, a microscopic imaging module 2, a receiving end 3, a sample 4 and an excitation light source 5, wherein the microsphere 1 is placed on the upper surface of the sample 4, the excitation light source 5 is located below the sample 4, the microsphere 1 is covered with a cover glass 6, and the microscopic imaging module 2 is located right above the excitation light source 5;
the microscopic camera module 2 comprises a first lens group 21, a second lens group 22 and an image sensor 23, wherein the first lens group 21 and the second lens group 22 are symmetrically arranged, the first lens group 21 is located between the second lens group 22 and the cover glass 6, the second lens group 22 is located between the first lens group 21 and the image sensor 23, and the image sensor 23 is electrically connected with the receiving end 3.
The second lens group 22 includes at least two microlenses, the size of the microlens near the image sensor 23 is the largest, and the size of the microlens far from the image sensor 23 is the smallest; the first lens group 21 includes at least two microlenses, a size of the microlens close to the image sensor 23 being smallest, and a size of the microlens far from the image sensor 23 being largest; the size of the micro lens is 0.5-15 mm in diameter.
The microsphere 1 is a transparent microsphere, and the diameter of the microsphere 1 is 5-300 microns.
The microsphere 1 is a barium titanate microsphere or a polystyrene microsphere. The optical path can play a role in amplifying and remarkably improving the resolution. The specific use mode of the microsphere is that the microsphere is tightly attached to a sample and observed through a microscopic camera module, and the specific implementation method comprises but is not limited to: directly placed on the surface of the sample, adhered to the objective lens and pressed on the sample, and the microspheres are fixed by a frame with small holes and tightly attached to the sample.
The microlenses of the smallest diameter of the first lens group 21 and the microlenses of the smallest diameter of the second lens group 22 are different microlenses.
The focal plane of the second lens group 22 coincides with the receiving end face of the image sensor 23, the focal length of the second lens group 22 is 1-3mm, and the F number of the second lens group 22 is less than 3.
The focal length of the first lens group 21 is 1-8mm, and the F number of the first lens group is less than 3.
The pixel size of the image sensor 23 is 0.8-2.5 μm.
The image sensor 23 is a CMOS image sensor or a CCD image sensor.
The excitation light source 5 is a laser light source, an LED light source or a gas light source.
The receiving end 3 is a computer, a mobile phone or an embedded system.
Example 2: a microsphere-based transmission high resolution microscopy imaging system, as shown in fig. 2, otherwise identical to that of example 1, except that the smallest diameter microlenses of the first lens group are the same microlenses as the smallest diameter microlenses of the second lens group.
The working principle of the utility model is as follows:
when the microscope system is used, all parts in the microscope system are well built, a sample is placed on a sample table between an excitation light source and a microscope camera module, microspheres cover the surface of the sample, a cover glass is placed on the sample, a power supply is turned on, an image is observed from a receiving end, fine adjustment focusing is carried out on the height of the whole body formed by the first lens group, the second lens group and the image sensor, the brightness of the excitation light source is adjusted, the observed image is made to be clearest, and the obtained image is stored at the receiving end.
The above only is the embodiment of the present invention, not limiting the scope of the present invention, all the equivalent structure changes made in the specification and the attached drawings or directly or indirectly applied to other related technical fields are included in the same principle as the present invention.
Claims (10)
1. A transmission-type high-resolution microscopic imaging system based on microspheres is characterized in that: the device comprises a microsphere (1), a microscopic camera module (2), a receiving end (3), a sample (4) and an excitation light source (5), wherein the microsphere is placed on the upper surface of the sample, the excitation light source is positioned below the sample, a cover glass (6) covers the microsphere, and the microscopic camera module is positioned right above the excitation light source;
the microscopic camera module comprises a first lens group (21), a second lens group (22) and an image sensor (23), wherein the first lens group and the second lens group are symmetrically arranged, the first lens group is located between the second lens group and the cover glass, the second lens group is located between the first lens group and the image sensor, and the image sensor is electrically connected with the receiving end.
2. The microsphere-based transmissive high-resolution microscopy imaging system of claim 1, wherein: the second lens group comprises at least two micro lenses, wherein the size of the micro lens close to the image sensor is the largest, and the size of the micro lens far away from the image sensor is the smallest; the first lens group comprises at least two micro lenses, the size of the micro lens close to the image sensor is the smallest, and the size of the micro lens far away from the image sensor is the largest; the size of the micro lens is 0.5-15 mm in diameter.
3. The microsphere-based transmissive high-resolution microscopy imaging system of claim 1, wherein: the microspheres are transparent microspheres, and the diameter of the microspheres is 5-300 microns.
4. The microsphere-based transmissive high-resolution microscopy imaging system of claim 1, wherein: the microspheres are barium titanate microspheres or polystyrene microspheres.
5. The microsphere-based transmissive high-resolution microscopy imaging system of claim 2, wherein: the micro lens with the smallest diameter of the first lens group and the micro lens with the smallest diameter of the second lens group are different micro lenses or the same micro lens.
6. The microsphere-based transmissive high-resolution microscopy imaging system of claim 1, wherein: the focal plane of the second lens group is superposed with the receiving end face of the image sensor, the focal length of the second lens group is 1-3mm, and the F number of the second lens group is less than 3.
7. The microsphere-based transmissive high-resolution microscopy imaging system of claim 1, wherein: the focal length of the first lens group is 1-8mm, and the F number of the first lens group is less than 3.
8. The microsphere-based transmissive high-resolution microscopy imaging system of claim 1, wherein: the pixel size of the image sensor is 0.8-2.5 μm.
9. The microsphere-based transmissive high-resolution microscopy imaging system of claim 1, wherein: the image sensor is a CMOS image sensor or a CCD image sensor.
10. The microsphere-based transmissive high-resolution microscopy imaging system of claim 1, wherein: the excitation light source is a laser light source, an LED light source or a gas light source.
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| CN201921305213.XU CN210323559U (en) | 2019-08-13 | 2019-08-13 | Microsphere-based transmission type high-resolution microscopic imaging system |
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| CN201921305213.XU CN210323559U (en) | 2019-08-13 | 2019-08-13 | Microsphere-based transmission type high-resolution microscopic imaging system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113037966A (en) * | 2021-02-27 | 2021-06-25 | 重庆市天实精工科技有限公司 | Micro-camera module and mobile terminal |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113037966A (en) * | 2021-02-27 | 2021-06-25 | 重庆市天实精工科技有限公司 | Micro-camera module and mobile terminal |
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