CN216082245U - Microscopical appearance device and cell phenotype controlling means - Google Patents

Abstract

The utility model provides a microscopical appearance device and cell phenotype controlling means, include: a molding plate having a plurality of molding holes penetrating two opposite surfaces of the molding plate for molding a plurality of solid media; a sample cell plate having a plurality of sample wells extending through two opposing surfaces of the sample cell plate for receiving a plurality of solid culture media; the cover glass is used for being placed on one surface of the sample cell plate; and the pressing plate is provided with a plurality of pressing columns and is used for pressing a plurality of solid culture mediums in the plurality of forming holes into the plurality of sample holes and pressing the solid culture mediums on the cover glass. The utility model can realize the preparation of a plurality of solid culture mediums at one time, can complete the tabletting operation of a plurality of samples at one time, saves time, effectively improves the experimental efficiency, has simple, convenient and quick operation, is not easy to cause misoperation, and is not easy to crush the cover glass.

Description

Microscopical appearance device and cell phenotype controlling means

Technical Field

The utility model relates to the technical field of microscopic experiments, in particular to a microscopic sample preparation device and a cell phenotype control device.

Background

In order to better observe single bacteria or cells on a microscope during biological or medical experiments, the following sample preparation procedures are often adopted: dropping the bacteria/cell suspension onto a cut solid medium (e.g., an agar block); after the surface of the agar is slightly dried, pressing the surface of the agar block on which the suspension is dripped on a glass cover slide; then placed on an inverted microscope to observe the bacteria or cells. The process described above may be referred to as a tableting process.

Above-mentioned experimentation needs the cutting agar-agar piece, drip bacterium/cell suspension on the agar-agar piece, will drip the agar-agar piece pressure that has the suspension and wait manual operation on the glass coverslip, the experimenter must be careful operation, just can obtain the better film-making sample of effect, for example probably crushing glass coverslip, in addition, when carrying out the preforming to a large amount of experimental samples, need operate one by one, whole experiment process is long consuming time, low efficiency, consume experimenter's plenty of time and energy, and the misoperation's condition appears easily, scientific research or medical diagnosis's progress has seriously been influenced.

SUMMERY OF THE UTILITY MODEL

In order to solve the problems identified in the background art, embodiments of the present invention provide a microscopical apparatus and a cell phenotype control apparatus.

According to an aspect of an embodiment of the present invention, there is provided a microsampling apparatus including: a forming plate having a plurality of forming holes penetrating two opposite surfaces of the forming plate for forming a plurality of solid media; a sample cell plate having a plurality of sample wells extending through two opposing surfaces of the sample cell plate for receiving a plurality of the solid culture media; a cover glass for placing on a surface of the sample cell plate; and the pressing plate is provided with a plurality of pressing columns and is used for pressing a plurality of solid culture mediums in the plurality of forming holes into the plurality of sample holes and pressing the sample holes on the cover glass.

In some embodiments, further comprising: a first glass sheet for placement on the bottom surface of the forming plate; the choke coil is placed on the top surface of the forming plate to form a pouring groove for pouring a liquid culture medium together with the top surface of the forming plate, and the pouring groove is communicated with the forming holes; a second glass piece for placing on the top surface of the forming plate after removing the chokes to form the solid medium in cooperation with the first glass plate and the forming holes.

In some embodiments, the microsampling device further comprises: the receiver, including the open and be used for placing in top the chamber of accomodating of profiled sheeting, be used for supporting the bottom sprag frame of profiled sheeting and be used for right the spacing frame of lateral part of the outer peripheral edge of profiled sheeting.

In some embodiments, further comprising: and the compression ring is used for compressing the cover glass on the surface of the sample cell plate.

In some embodiments, the compression ring and the sample cell plate are fixed by means of magnetic attraction.

In some embodiments, further comprising: the black adhesive pad is used for adhering the cover glass to the surface of the sample cell plate, the adhesive pad is provided with a plurality of openings penetrating through two opposite surfaces of the adhesive pad, and the plurality of openings are used for corresponding to the plurality of sample holes one by one.

In some embodiments, further comprising: the positioning tray comprises a top opening and is used for placing the containing cavity of the sample cell plate, a positioning plate and a positioning plate, wherein the positioning plate is used for supporting the cover glass and the middle supporting bulge of the sample cell plate and a positioning plate used for positioning the cover glass and the outer peripheral limiting bulge of the sample cell plate.

In some embodiments, further comprising: the drying rack is provided with at least one left side supporting block and at least one right side supporting block, the left side supporting block and the right side supporting block are arranged at intervals in a left-right mode and are in one-to-one correspondence, and slots for inserting the forming plates are formed in one sides, facing each other, of the left side supporting block and the right side supporting block respectively.

In some embodiments, the pressing plate is further provided with a handle, and the handle and the pressing column are respectively arranged on two opposite surfaces of the pressing plate.

In some embodiments, further comprising: the moisture preservation plate is provided with a plurality of through holes and is used for being communicated with the sample holes in a one-to-one correspondence mode, a water storage groove is formed in one surface of the moisture preservation plate, the through holes are communicated with the water storage groove, and the through holes are separated from the water storage groove through a flow choking ring wall; and the transparent cover plate is used for covering the surface of the moisturizing plate so as to close the water storage tank.

In some embodiments, further comprising: the middle connecting plate is provided with a plurality of through holes which are in one-to-one correspondence with the plurality of forming holes, and the middle connecting plate is used for detachably connecting the forming plates with the cell culture plate of the cell phenotype control device.

According to another aspect of the embodiments of the present invention, there is provided a cell phenotype control apparatus, comprising a cell culture plate and the microsampling apparatus of the previous embodiments.

The embodiment of the utility model has the beneficial effects that:

1. according to the embodiment of the utility model, the forming plate with the plurality of forming holes, the sample pool plate with the plurality of sample holes and the pressing plate with the plurality of pressing columns are arranged, so that a plurality of solid culture mediums can be prepared at one time, the pressing operation of a plurality of samples can be completed at one time, the time is saved, the experimental efficiency is effectively improved, the operation is simple, convenient and rapid, the condition of misoperation is not easy to occur, and the cover glass is not easy to crush;

2. according to the embodiment of the utility model, the first glass sheet, the second glass sheet and the forming plate are arranged to form the solid culture medium together, so that the surface of the solid culture medium is ensured to be flat, and the culture medium with higher surface quality is provided for the suspension liquid;

3. according to the embodiment of the utility model, the storage box is arranged, so that the liquid culture medium can be stored, a good supporting effect can be achieved on the forming plate when the solid culture medium is prepared, and the forming plate, the first glass sheet and the second glass sheet can be positioned and aligned;

4. according to the embodiment of the utility model, the drying rack is arranged to basically dry the solid culture medium before the suspension liquid is dripped, so that the drying time in the suspension liquid dripping process is shortened, a large amount of water is prevented from being generated in the subsequent tabletting process, and the condition that microorganisms in the suspension liquid cannot meet the experimental requirements due to the migration of the microorganisms with the water is avoided;

5. in the embodiment of the utility model, the moisturizing part is arranged to moisturize the solid culture medium in the sample pool plate, so that the solid culture gene is prevented from being dehydrated and deformed to be separated from a cover glass, and an experimenter can conveniently carry out microscopic observation for a long time;

6. according to the embodiment of the utility model, the molding plate and the cell culture plate are detachably connected through the middle connecting plate, so that a set of cell phenotype control device convenient to disassemble and assemble is formed, and the optogenetics experiment is convenient to carry out.

Specific embodiments of the present invention are disclosed in detail with reference to the following description and drawings, indicating the manner in which the principles of the utility model may be employed. It should be understood that the embodiments of the utility model are not so limited in scope. The embodiments of the utility model include many variations, modifications and equivalents within the spirit and scope of the appended claims.

Drawings

The accompanying drawings, which are included to provide a further understanding of embodiments of the utility model and are incorporated in and constitute a part of this specification, illustrate embodiments of the utility model and together with the description serve to explain the principles of the utility model. It is obvious that the drawings in the following description are only some embodiments of the utility model, and that for a person skilled in the art, other drawings can be derived from them without inventive effort. In the drawings:

FIG. 1 is a schematic diagram of an example of a portion of the components of a microsampling device according to an embodiment of the present invention;

FIG. 2 is a schematic view of an example of a molding plate of the microsampling apparatus according to the embodiment of the present invention;

FIG. 3 is a schematic view of an example of a sample cell plate of a microscopical assembly according to an embodiment of the present disclosure;

FIG. 4 is a schematic view of an example of a blocker coil of a microsampling device according to an embodiment of the present disclosure;

FIG. 5 is a schematic view of an example of a storage cassette of the microsampling apparatus according to the embodiment of the present invention;

FIG. 6 is a schematic diagram of one example of a sample preparation assembly of a microscopy sample preparation device according to an embodiment of the utility model;

FIGS. 7-10 are schematic diagrams of the assembly of the micro-sampling device of the embodiment of the present invention during the sampling process;

FIG. 11 is a cross-sectional view of the assembly of FIG. 10;

FIGS. 12 and 13 are schematic views of an example of a drying rack of a microscopical assembly according to an embodiment of the present invention;

FIGS. 14-16 are schematic diagrams of one example of a sample cell plate assembly of a microscopy sample device according to an embodiment of the utility model;

FIG. 17 is a schematic view of an adhesive pad of a microscopy device according to an embodiment of the utility model positioned between a sample cell plate and a cover slip;

FIG. 18 is a schematic view of an example of a positioning tray of the microscopical device of the embodiment of the present invention;

FIGS. 19 and 20 are schematic views of a sample cell plate assembly of a microscopical assembly according to an embodiment of the present invention placed in a positioning tray;

FIGS. 21 and 22 are schematic views of a sample cell plate assembly and a forming plate of a microsampling device according to an embodiment of the present invention placed on a positioning tray;

FIG. 23 is a schematic view of an example of a platen of a microsampling device according to an embodiment of the present invention;

FIGS. 24 and 25 are schematic diagrams of the mating of components of a microsample assembly of an embodiment of the present invention during a sheeting process;

FIGS. 26 and 27 are schematic illustrations of the mating of parts of a microscopical device of an embodiment of the present invention after compression;

FIG. 28 is a schematic view of an example of a moisturizing member of a microscopy sample device according to an embodiment of the utility model;

FIGS. 29 and 30 are schematic views of a moisturizing member of a microscopy sample device according to an embodiment of the utility model in cooperation with a sample well plate;

FIG. 31 is a schematic view of an example of an intermediate plate of the microscopical assembly of an embodiment of the present invention;

fig. 32 and 33 are schematic views of an example of a cell phenotype control apparatus according to an embodiment of the present invention.

Main element number description:

101. forming a plate; 102. forming holes; 103. a sample cell plate; 104. a cover glass; 105. pressing a plate;

106. a sample well; 107. pressing the column; 108. a handle; 109. a positioning pin mounting hole; 110. positioning holes;

111. a first glass sheet; 112. a choke coil; 113. a second glass sheet; 114. a first magnet mounting hole;

115. a second magnet mounting hole; 116. a storage box; 117. a receiving cavity; 118. a bottom support frame;

119. a side limiting frame; 120. operating an avoidance slot; 121. pressing a ring; 122. a third magnet mounting hole;

123. a limiting convex rib; 124. positioning the tray; 125. an accommodating chamber; 126. a middle support boss;

127. an outer limit bulge; 128. airing the rack; 129. a left side support block; 130. a right support block;

131. a slot; 132. a lower support plate; 133. an upper support plate; 134. a column; 135. a moisturizing member;

136. a moisture retention sheet; 137. a transparent cover plate; 138. through holes; 139. a water storage tank; 140. a choke annular wall;

141. a middle connection plate; 142. a through hole; 143. an adhesive pad; 144. positioning pins;

200. a solid medium; 300. a cell culture plate; 301. micropores; 400. a light source control box.

Detailed Description

The foregoing and other features of the utility model will become apparent from the following description taken in conjunction with the accompanying drawings. In the description and drawings, particular embodiments of the utility model have been disclosed in detail as being indicative of some of the embodiments in which the principles of the utility model may be employed, it being understood that the utility model is not limited to the embodiments described, but, on the contrary, is intended to cover all modifications, variations, and equivalents falling within the scope of the appended claims.

In the embodiments of the present invention, the terms "first", "second", and the like are used to distinguish different elements from each other by reference, but do not indicate a spatial arrangement or a temporal order of the elements, and the elements should not be limited by the terms. The term "and/or" includes any and all combinations of one or more of the associated listed terms. The terms "comprising," "including," "having," and the like, refer to the presence of stated features, elements, components, and do not preclude the presence or addition of one or more other features, elements, components, and elements.

In embodiments of the utility model, the singular forms "a", "an", and the like may include the plural forms and are to be construed broadly as "a" or "an" and not limited to the meaning of "a" or "an"; furthermore, the term "comprising" should be understood to include both the singular and the plural, unless the context clearly dictates otherwise; further, the term "according to" should be understood as "at least partially according to … …," and the term "based on" should be understood as "based at least in part on … …," unless the context clearly dictates otherwise; further, the term "plurality" means two or more unless otherwise specified.

In embodiments of the present invention, the use of adjective or adverbial modifiers "upper" and "lower," "top" and "bottom," "inner" and "outer," "front" and "back" are merely to facilitate relative reference between groups of terms, and do not describe any particular directional limitation on the modified terms.

Embodiments of the present invention will be described below with reference to the drawings.

Embodiments of the first aspect

Embodiments of a first aspect of the utility model provide a microsampling device.

The microscopic sample preparation device of the embodiment of the utility model comprises a sample preparation component and a tabletting component, as shown in fig. 1, the sample preparation component comprises a forming plate 101, the forming plate 101 is provided with a plurality of forming holes 102 which respectively penetrate through two opposite surfaces of the forming plate 101, the plurality of forming holes 102 are used for forming a plurality of solid culture mediums 200 (as shown in fig. 11), and the single preparation of the plurality of solid culture mediums 200 is realized, for example, the solid culture mediums 200 are agar blocks; as shown in fig. 14 and 23, the tabletting assembly comprises a sample cell plate 103, a cover glass 104 and a pressing plate 105, wherein the sample cell plate 103 has a plurality of sample holes 106 penetrating two opposite surfaces of the sample cell plate 103, the plurality of sample holes 106 can correspond to the plurality of molding holes 102 one by one, the cover glass 104 is used for being placed on one surface of the sample cell plate 103, the pressing plate 105 is provided with a plurality of pressing columns 107, the plurality of pressing columns 107 can correspond to the plurality of molding holes 102 one by one for pressing the plurality of solid mediums 200 in the plurality of molding holes 102 into the plurality of sample holes 106 and onto the cover glass 104, for example, the pressing plate 105 is further provided with a handle 108, and the handle 108 and the pressing columns 107 are respectively arranged on two opposite sides of the pressing plate 105 so as to press the pressing plate 105 by holding the handle 108.

The sample preparation by the microscopic sample preparation device of the embodiment can comprise the following steps:

step S100: preparation of solid medium (e.g., agar block): as shown in fig. 11, a liquid medium (e.g., agar liquid) is poured into a plurality of molding holes 102 of a molding plate 101, and the liquid medium is cooled and solidified to form a solid medium 200;

step S200: dripping: dropping the bacteria/cells suspension on the surface (called front) of a plurality of solid media 200;

step S300: tabletting: as shown in fig. 24 to 27, the cover glass 104 is placed on the bottom surface of the sample cell plate 103, the molding plate 101 is placed on the top surface of the sample cell plate 103 (the front surface of the solid medium 200 faces downward), the pressing plate 105 is placed on the molding plate 101, at this time, the pressing columns 107, the molding holes 102 and the sample holes 106 are in one-to-one correspondence from top to bottom, the pressing plate 105 is pressed downward until the pressing columns 107 press the plurality of solid mediums 200 in the plurality of molding holes 102 into the plurality of sample holes 106 and onto the cover glass 104, so that a plurality of microscopic samples with bacteria/cell suspension liquid drops contacting the cover glass 104 can be obtained, and then the sample cell plate 103 can be placed on a microscope platform, and the suspension liquid drops on the solid mediums 200 can be observed through the cover glass 104.

Adopt the micro-system appearance device of this embodiment can realize once preparing a plurality of solid medium, once only accomplishes the preforming operation of a plurality of samples, saves time, effectively improves experimental efficiency, and easy operation is convenient, is difficult to the condition of misoperation, is difficult to the crushing coverslip.

Further, the height of the pressing column 107 should not be less than the height of the molding hole 102, so as to press the solid medium 200 out of the molding hole 102 and into the sample hole 106.

Further, the diameter of the sample well 106 is larger than the diameter of the molding well 102, so that when the solid medium 200 is pressed into the sample well 106, the edge of the solid medium 200 is prevented from being damaged by contact with the wall of the sample well 106, and a pressure-holding effect due to gas remaining below the solid medium 200 pressed into the sample well 106 is also prevented.

In some embodiments, as shown in fig. 2, 3, and 23, forming plate 101, sample cell plate 103, and pressure plate 105 may all be square plates, and forming plate 101, sample cell plate 103, and pressure plate 105 may have the same length dimension and the same width dimension. The plurality of molding holes 102 on the molding plate 101 may be arranged in a plurality of rows and columns, for example, in 8 rows and 12 columns, i.e., the number of the molding holes 102 may be 96, and the arrangement and number of the sample holes 106 and the arrangement and number of the press pillars 107 are consistent with the arrangement and number of the molding holes 102, so as to form a one-to-one correspondence relationship.

Further, as shown in fig. 2, hole position marks may be disposed at two vertical sides of the molding plate 101, for example, a number mark (e.g., 1-12) is used in the length direction, and an alphabet mark (e.g., a-H) is used in the width direction, so as to describe the positions of the molding holes 102, i.e., to describe the positions of the samples.

Further, as shown in fig. 3 and 20, positioning pin mounting holes 109 may be respectively provided at two opposite side edges of the sample cell plate 103 to fix the positioning pins 144, and correspondingly, as shown in fig. 2, 20 and 21, positioning holes 110 may be respectively provided at two opposite side edges of the forming plate 101 to be inserted into and positioned by the positioning pins 144, so that in the sheet pressing step of step S300, the relative positions of the sample cell plate 103 and the forming plate 101 are positioned to be centered.

In some embodiments, as shown in fig. 6, the sample preparation assembly may further include a first glass plate 111, a choke coil 112, and a second glass plate 113, for example, the first glass plate 111 and the second glass plate 113 are ultra-thin glass with a thickness of less than 0.2 mm, the first glass plate 111 is configured to be placed on the bottom surface of the forming plate 101 to close the lower end of the forming hole 102, the choke coil 112 is configured to be placed on the top surface of the forming plate 101, that is, the choke coil 112 surrounds the outer sides of the plurality of forming holes 102 to form a casting groove for casting the liquid culture medium together with the top surface of the forming plate 101, and the casting groove is communicated with the plurality of forming holes 102; the second glass sheet 113 is used for being placed on the top surface of the forming plate 101 after the choke coil 112 is removed, so as to form a sample together with the first glass plate and the forming hole 102, ensure the surface of the solid culture medium to be flat, and provide the culture medium with higher surface quality for the dripping suspension.

When the microsample preparation device of this embodiment is used for preparing a sample, in the step of preparing the solid medium in step S100, the method may specifically include:

step S110: as shown in fig. 7 and 8, the first glass sheet 111 is placed on the bottom surface of the forming plate 101, and the choke coil 112 is placed on the top surface of the forming plate 101;

step S120: pouring a liquid culture medium into a pouring groove on the inner side of the choke coil 112 (for example, a large-volume pipette is adopted), wherein the liquid culture medium flows into the plurality of forming holes 102 from the pouring groove, the injection amount of the liquid culture medium needs to ensure that after the plurality of forming holes 102 are filled with the liquid culture medium, the liquid level of the liquid culture medium is still higher than the top surface of the forming plate 101, after pouring is completed, whether each forming hole 102 is filled with the liquid culture medium is observed, if the forming holes are not filled with the liquid culture medium, the forming plate 101 can be slightly shaken to ensure that the liquid culture medium is uniformly distributed, and the plurality of forming holes 102 are filled with the liquid culture medium;

step S130: after the plurality of molding holes 102 are filled with the liquid culture medium, the choker 112 is taken away from the top surface of the molding plate 101;

step S140: as shown in fig. 9, the second glass sheet 113 is slowly laid down against one side of the forming plate 101, so that the second glass sheet 113 contacts and infiltrates the liquid medium on the top surface of the forming plate 101, ensuring that no air bubbles exist until the second glass sheet 113 is entirely adhered to the top surface of the forming plate 101;

step S150: as shown in fig. 10 and 11, the second glass sheet 113 is pressed down with uniform force to extrude a layer of liquid culture medium remaining between the second glass sheet 113 and the top surface of the forming plate 101, so that as little liquid culture medium as possible remains between the forming plate 101 and the second glass sheet 113, and both end surfaces of the solid culture medium 200 formed in each forming hole 102 are flat;

step S160: after the liquid culture medium in the molding hole 102 is cooled and solidified, the residual agar at the edge of the molding plate 101 is cleaned, and then the first glass sheet 111 and the second glass sheet 113 are respectively translated and pushed away along the bottom surface and the top surface of the molding plate 101, so that the cylindrical solid culture medium 200 with smooth two end surfaces is obtained.

Furthermore, the choker 112 and the forming plate 101 can be fixed and attached in a magnetic attraction manner, and the assembly and disassembly are very convenient. For example, specifically, as shown in fig. 4, at least two opposite sides of the choke coil 112 are provided with first magnet mounting holes 114 for mounting first magnets; as shown in fig. 2, at least two opposite side edges of the forming plate 101 are provided with second magnet mounting holes 115 for mounting second magnets, the second magnets correspond to the first magnets, when the choke ring 112 is disposed on the top surface of the forming plate 101, the first magnets and the second magnets are magnetically attracted, so that the choke ring 112 and the forming plate 101 are attached and fixed, and the magnets and the magnet mounting holes can be fixed in an interference fit manner.

In order to facilitate the taking and placing of the choke coil 112, side lugs (as shown in fig. 4 and 8) may be disposed on two opposite side edges of the choke coil 112, and when the choke coil 112 is disposed on the top surface of the forming plate 101, the side lugs are located on the outer side of the forming plate 101 instead of being attached to the forming plate 101.

Further, the first glass piece 111, the choke coil 112, and the second glass piece 113 may each have a square shape conforming to the shape of the forming plate 101.

In some embodiments, as shown in fig. 5, the microsampling device may further include a storage box 116 for supporting and positioning the forming plate 101 during preparation of the solid culture medium 200, and for storing the overflowing liquid culture medium, the storage box 116 has a storage cavity 117 with an open top for placing the forming plate 101, a bottom support frame 118 for supporting the forming plate 101, and a side limiting frame 119 for limiting the outer periphery of the forming plate 101, in the example of fig. 5, the bottom support frame 118 and the side limiting frame 119 are both disposed in the storage cavity 117, for example, the bottom support frame 118 and the side limiting frame 119 are both rib plates, and an operation avoiding groove 120 may be disposed on the outer peripheral wall of the storage box 116 to facilitate placement of the forming plate 101 and other components.

When the microsample preparation device of this embodiment is used for sample preparation, in step S110, as shown in fig. 6 to 8, the first glass sheet 111, the forming plate 101, and the choke coil 112 may be sequentially stacked on the bottom support 118 in the accommodating cavity 117 from bottom to top, and the lateral limiting frame 119 limits horizontal positions of the first glass sheet 111, the forming plate 101, and the choke coil 112, so that the first glass sheet 111, the forming plate 101, and the choke coil 112 are centered and aligned. In step S150, as shown in FIG. 9, when the second glass plate 113 is pressed down, the pushed-out liquid medium flows into the housing chamber 117.

In some embodiments, as shown in fig. 14 to 16, the microscopy device may further include a pressing ring 121, where the pressing ring 121 is used to press the cover glass 104 against the surface of the sample cell plate 103, so that the cover glass 104 and the surface of the sample cell plate 103 are tightly attached.

In the sample preparation using the microscopical sampling apparatus of the present embodiment, in the pressing step of step S300, after the cover glass 104 is placed on the bottom surface of the sample cell plate 103, the pressing ring 121 is placed on the bottom surface of the cover glass 104, so that the cover glass 104 and the sample cell plate 103 are fixed by the pressing ring 121.

In a possible technical solution, the pressing ring 121 and the sample cell plate 103 are fixed by a magnetic attraction manner. Specifically, the pressing ring 121 is made of a metal capable of being attracted by a magnet, at least two opposite side edges of the sample cell plate 103 are provided with third magnet mounting holes 122 (as shown in fig. 3) for mounting a third magnet, and when the pressing ring 121 is disposed on the surface of the cover glass 104 opposite to the sample cell plate 103, the third magnet and the pressing ring 121 are magnetically attracted (as shown in fig. 15), so that the cover glass 104 and the sample cell plate 103 are tightly attached, and the assembly and disassembly are convenient.

Further, as shown in fig. 3, two limiting convex ribs 123 may be provided on the surface of the sample cell plate 103 for mounting the cover glass 104, and the two limiting convex ribs 123 are respectively provided at two opposite side edges of the sample cell plate 103, so as to limit the cover glass 104 in the left-right direction on one hand and limit the pressing ring 121 in the left-right direction on the other hand.

In other embodiments, as shown in fig. 17, the microscopical device may further include a black adhesive pad 143, the adhesive pad 143 is used to adhere the cover glass 104 to the surface of the sample cell plate 103, the adhesive pad 143 has a plurality of openings penetrating through two opposite surfaces of the adhesive pad 143, and the plurality of openings are in one-to-one communication with the plurality of sample wells 106 when the adhesive pad 143 is adhered to the surface of the sample cell plate 103.

Compared with the clamping ring fixing method in the previous embodiment, the sample cell plate 103 is well adhered to the cover glass 104 and the sample cell plate 103 with smooth surface by the adhesive pad 143, and the adhesive pad 143 is easily separated from the surface of the cover glass 104 and the surface of the sample cell plate 103 after encountering water, and can be repeatedly adhered. The adhesive pad 143 is as thin as possible and is black in color to ensure that there is no light leakage between the holes during optogenetic experiments.

However, the present invention is not limited thereto, and the pressing ring 121 and the sample cell plate 103 may be fixed by other conventional connection means that facilitates the detachment and installation.

In some embodiments, as shown in fig. 18, the microscopical apparatus may further include a positioning tray 124, the positioning tray 124 includes a receiving cavity 125 with an open top for placing the sample cell plate 103, a middle supporting protrusion 126 for supporting the cover glass 104 and the sample cell plate 103, and an outer limiting protrusion 127 for limiting the outer periphery of the cover glass 104 and the sample cell plate 103, and when the sample cell assembly composed of the cover glass 104, the sample cell plate 103 and the press ring 121 is placed on the middle supporting protrusion 126 (as shown in fig. 19 and 20), the press ring 121 is suspended outside the middle supporting protrusion 126 rather than being attached to the middle supporting protrusion 126. Wherein the surface of the middle support protrusion 126 is flat to provide good support for the cover glass 104.

In the sheeting step of step S300, the cuvette assembly may be placed on the middle support protrusion 126 of the positioning tray 124 with the press ring 121 facing downward (as shown in fig. 18 to 20), and the molding plate 101 may be placed on the top surface of the cuvette plate 103 with the front surface of the solid medium 200 facing downward (as shown in fig. 21 and 22), and at this time, the outer side limit protrusion 127 of the positioning tray 124 may limit the outer peripheral edges of the cuvette assembly and the molding plate 101, so that the cuvette plate 103 and the molding plate 101 may be aligned to prevent the displacement therebetween. Since the cover glass 104 is supported by the central support projection 126, the cover glass 104 is less likely to be deformed and damaged when the pressing plate 105 is pressed down.

In some embodiments, as shown in fig. 12, the microsampling device may further include an airing frame 128 for airing the solid medium 200 on the forming plate 101, the airing frame 128 has at least one left support block 129 and at least one right support block 130, the left support block 129 and the right support block 130 are arranged at a left-right interval and in one-to-one correspondence, and the sides of the left support block 129 and the right support block 130 facing each other are respectively provided with a slot 131 for inserting the forming plate 101, so that the forming plate 101 is aired in a suspended state on the airing frame 128, and the solid medium 200 in the forming hole 102 is prevented from being deformed due to contact with the airing frame 128.

When the microsampling device of this embodiment is used to prepare a sample, the step of preparing a solid medium in step S100 may further include step S170: as shown in FIG. 13, the molding plate 101 is placed on the airing rack 128 to air-dry the solid medium 200. After the solid culture medium 200 is basically dried, the suspension is dripped on the solid culture medium 200, so that the drying time in the suspension dripping process is shortened, a large amount of water is prevented from being generated in the subsequent tabletting process, and the situation that microorganisms in the suspension cannot meet the experimental requirements due to the migration of the microorganisms with the water is avoided.

Further, as shown in fig. 12 and 13, the drying rack 128 may have a plurality of left support blocks 129 and a plurality of right support blocks 130, the plurality of left support blocks 129 are disposed at intervals in the height direction of the drying rack 128, and correspondingly, the plurality of right support blocks 130 are disposed at intervals in the height direction of the drying rack 128 and are in one-to-one correspondence with the plurality of left support blocks 129 in the horizontal direction.

Further, as shown in fig. 12 and 13, the airing frame 128 further includes a lower supporting plate 132, an upper supporting plate 133, and a plurality of vertical posts 134 (such as copper posts) connecting the lower supporting plate 132 and the upper supporting plate 133, for example, the lower supporting plate 132 and the upper supporting plate 133 are both square hollow frames, the number of the vertical posts 134 is four, the four vertical posts are respectively connected and supported from four corners of the lower supporting plate 132 and the upper supporting plate 133, and the left supporting block 129 and the right supporting block 130 can be fixed on the vertical posts 134.

In some embodiments, as shown in fig. 28, 29 and 30, the microscopical device may further include a moisture retention member 135, the moisture retention member 135 includes a moisture retention plate 136 and a transparent cover plate 137, the moisture retention plate 136 has a plurality of through holes 138 penetrating two opposite surfaces of the moisture retention plate 136, the plurality of through holes 138 are used for communicating with the plurality of sample holes 106 in a one-to-one correspondence, one surface of the moisture retention plate 136 is provided with a water reservoir 139, the plurality of through holes 138 are communicated with the water reservoir 139 to allow water vapor in the water reservoir 139 to enter the through holes 138, the plurality of through holes 138 are separated from the water reservoir 139 by a blocker ring wall 140 to limit water in the water reservoir 139 from flowing into the through holes 138; the transparent cover 137 may be a glass cover for covering the surface of the moisture-retaining plate 136 to close the reservoir 139.

During the experiment, when the microscopic sample needs to be observed for a long time, the moisturizing member 135 can be used to moisturize the solid medium 200 in the sample cell plate 103, so as to prevent the solid medium 200 from being separated from the cover glass 104 due to dehydration deformation (such as shrinking toward the middle).

When the microscopic sample preparation device of the embodiment is used for sample preparation, the method may further include step S400: as shown in fig. 29 and fig. 30, the lower surface of the moisture-keeping plate 136 facing away from the water storage groove 139 is placed in contact with the surface of the sample cell plate 103 facing away from the cover glass 104, at this time, the water storage groove 139 faces upward, water is added into the water storage groove 139, the transparent cover plate 137 is covered on the upper surface of the moisture-keeping plate 136 to seal the water storage groove 139, so that a closed moisture-keeping space is obtained, moisture in the water storage groove 139 evaporates to keep a certain humidity in the moisture-keeping space, and the solid culture medium 200 in the sample cell plate 103 is effectively prevented from being dehydrated.

In addition, because the through holes 138 correspond to the sample holes 106 one by one, and the transparent cover 137 is made of transparent material, the light source above the microscope device can be ensured to irradiate on the solid medium 200 smoothly, and the normal operation of the optical experiment is not affected.

In some embodiments, as shown in fig. 31, the microscopical device further comprises an intermediate plate 141, the intermediate plate 141 is provided with a plurality of through holes 142 corresponding to the plurality of molding holes 102, and the intermediate plate 141 is used for detachably connecting the molding plate 101 and the cell culture plate 300 of the cell phenotype control device (as shown in fig. 32), so that the microscopical device and the cell phenotype control device form a set of device which is convenient to assemble and disassemble. When the shaping plate 101 and the cell culture plate 300 are connected through the middle connection plate 141, the plurality of shaping holes 102 on the shaping plate 101, the plurality of sample holes 106 on the sample well plate 103, the plurality of through holes 142 on the middle connection plate 141, and the plurality of micro holes 301 on the cell culture plate 300 correspond one to one.

The middle connection plate 141 and the molding plate 101 may be connected by magnetic attraction, and the middle connection plate 141 and the cell culture plate 300 may be connected by interference fit. For example, specifically, two opposite side edges of the middle connecting plate 141 are provided with magnet mounting holes for mounting a fourth magnet, and the fourth magnet corresponds to the second magnet of the forming plate 101, so as to magnetically fix the middle connecting plate 141 and the forming plate 101.

Embodiments of the second aspect

Embodiments of the second aspect of the present invention provide a cell phenotype control device, as shown in fig. 32 and 33, which includes a cell culture plate 300, a light source control box 400 and the microsampling device described in the embodiments of the first aspect. Since the structure of the microsampling device has been described in detail in the embodiment of the first aspect, the contents thereof are incorporated herein, and the description thereof is omitted here.

In the embodiment of the present invention, as for other structures of the cell phenotype control device, reference may be made to the related art, and the description thereof is omitted here. For example, reference may be made to the other structure of the cell phenotype control device in the Chinese patent application with the patent application No. CN 202011476070.6.

While the utility model has been described with reference to specific embodiments, it will be apparent to those skilled in the art that these descriptions are illustrative and not intended to limit the scope of the utility model. Various modifications and alterations of this invention will become apparent to those skilled in the art based upon the spirit and principles of this invention, and such modifications and alterations are also within the scope of this invention.

The preferred embodiments of the present invention have been described above with reference to the accompanying drawings. The many features and advantages of the embodiments are apparent from the detailed specification, and thus, it is intended by the appended claims to cover all such features and advantages of the embodiments that fall within the true spirit and scope thereof. Further, since numerous modifications and changes will readily occur to those skilled in the art, it is not desired to limit the embodiments of the utility model to the exact construction and operation illustrated and described, and accordingly, all suitable modifications and equivalents may be resorted to, falling within the scope thereof.

Claims (12)

1. A microscopical sample device, comprising:

a forming plate having a plurality of forming holes penetrating two opposite surfaces of the forming plate for forming a plurality of solid media;

a sample cell plate having a plurality of sample wells extending through two opposing surfaces of the sample cell plate for receiving a plurality of the solid culture media;

a cover glass for placing on a surface of the sample cell plate;

and the pressing plate is provided with a plurality of pressing columns and is used for pressing a plurality of solid culture mediums in the plurality of forming holes into the plurality of sample holes and pressing the sample holes on the cover glass.

2. The microsampling device of claim 1, further comprising:

a first glass sheet for placement on the bottom surface of the forming plate;

the choke coil is placed on the top surface of the forming plate to form a pouring groove for pouring a liquid culture medium together with the top surface of the forming plate, and the pouring groove is communicated with the forming holes;

a second glass piece for placing on the top surface of the forming plate after removing the chokes to form the solid medium in cooperation with the first glass piece and the forming holes.

3. The microsampling device of claim 2, wherein the microsampling device further comprises:

the receiver, including the open and be used for placing in top the chamber of accomodating of profiled sheeting, be used for supporting the bottom sprag frame of profiled sheeting and be used for right the spacing frame of lateral part of the outer peripheral edge of profiled sheeting.

4. The microsampling device of claim 1, further comprising:

and the compression ring is used for compressing the cover glass on the surface of the sample cell plate.

5. The microscopical sampling device of claim 4, wherein the clamping ring is fixed to the sample cell plate by magnetic attraction.

6. The microsampling device of claim 1, further comprising:

the black adhesive pad is used for adhering the cover glass to the surface of the sample cell plate, the adhesive pad is provided with a plurality of openings penetrating through two opposite surfaces of the adhesive pad, and the plurality of openings are used for corresponding to the plurality of sample holes one by one.

7. The microsampling device of claim 1, further comprising:

the positioning tray comprises a top opening and is used for placing the containing cavity of the sample cell plate, a positioning plate and a positioning plate, wherein the positioning plate is used for supporting the cover glass and the middle supporting bulge of the sample cell plate and a positioning plate used for positioning the cover glass and the outer peripheral limiting bulge of the sample cell plate.

8. The microsampling device of claim 1, further comprising:

the drying rack is provided with at least one left side supporting block and at least one right side supporting block, the left side supporting block and the right side supporting block are arranged at intervals in a left-right mode and are in one-to-one correspondence, and slots for inserting the forming plates are formed in one sides, facing each other, of the left side supporting block and the right side supporting block respectively.

9. The microsampling device of claim 1, wherein said pressure plate further comprises a handle, said handle and said compression leg being disposed on opposite surfaces of said pressure plate.

10. The microsampling device of claim 1, further comprising:

the moisture preservation plate is provided with a plurality of through holes and is used for being communicated with the sample holes in a one-to-one correspondence mode, a water storage groove is formed in one surface of the moisture preservation plate, the through holes are communicated with the water storage groove, and the through holes are separated from the water storage groove through a flow choking ring wall;

and the transparent cover plate is used for covering the surface of the moisturizing plate so as to close the water storage tank.

11. The microsampling device of claim 1, further comprising:

the middle connecting plate is provided with a plurality of through holes which are in one-to-one correspondence with the plurality of forming holes, and the middle connecting plate is used for detachably connecting the forming plates with the cell culture plate of the cell phenotype control device.

12. A cell phenotype control apparatus comprising the microsyringe apparatus of any one of claims 1 to 11 and a cell culture plate.

Images