CN216051764U

CN216051764U - Enzyme-linked immunosorbent assay sample adding device for detecting lipoprotein phospholipase A2

Info

Publication number: CN216051764U
Application number: CN202122646100.XU
Authority: CN
Inventors: 刘朋飞
Original assignee: Hebei Guogao Biotechnology Co ltd
Current assignee: Hebei Guogao Biotechnology Co ltd
Priority date: 2021-11-01
Filing date: 2021-11-01
Publication date: 2022-03-15
Anticipated expiration: 2031-11-01

Abstract

The utility model relates to the technical field of medical diagnosis, in particular to an enzyme-linked immunosorbent assay sample adding device for detecting lipoprotein phospholipase A2. The utility model can achieve the purpose of improving the liquid adding efficiency.

Description

Enzyme-linked immunosorbent assay sample adding device for detecting lipoprotein phospholipase A2

Technical Field

The utility model relates to the technical field of medical diagnosis, in particular to an enzyme-linked immunosorbent assay sample adding device for detecting lipoprotein phospholipase A2.

Background

Lipoprotein phospholipase A2(LP-PLA2) is synthesized and secreted by mature macrophages and lymphocytes, is regulated by inflammatory mediators, and has the effects of promoting inflammation and promoting atherosclerosis. Recent years have shown that it has an effect of promoting Atherosclerosis (AS), and is a new inflammatory response marker in AS. An enzyme-linked immunosorbent assay is commonly used in the detection process of lipoprotein phospholipase A2, and Chinese patent CN109870569A discloses an enzyme-linked immunosorbent assay auxiliary sample adding device, the scheme comprises a workbench and a mechanical arm, wherein the workbench is provided with a fixing frame, the mechanical arm comprises a first connecting rod, a second connecting rod, a first motor and a motor, and an output shaft of the first motor is connected with one end of the first connecting rod to realize the swing of the first connecting rod; an output shaft of the motor is connected with one end of the second connecting rod to realize the swing of the second connecting rod; the other end of second connecting rod sets firmly the drip ware, and this scheme liquid feeding is inefficient to the arm is formed by first connecting rod and second connecting rod are articulated, and the liquid feeding process motion is complicated, influences the detection efficiency that lipoprotein phospholipase A2 detected, to the above-mentioned problem that prior art exists, urgently needed an enzyme-linked immunosorbent assay application of sample device to lipoprotein phospholipase A2 detects to solve.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide an enzyme-linked immunosorbent assay sample adding device for detecting lipoprotein phospholipase A2, which aims to solve the problems and achieve the aim of improving the liquid adding efficiency.

In order to achieve the purpose, the utility model provides the following scheme: an enzyme-linked immunosorbent assay sample adding device for detecting lipoprotein phospholipase A2 comprises a bottom plate, wherein two first supporting plates are symmetrically and fixedly connected above the bottom plate, a transverse moving assembly is arranged between the two first supporting plates, the transverse moving assembly is fixedly connected with a longitudinal moving assembly, a liquid suction assembly is arranged at the edge part above the bottom plate, the liquid suction assembly is connected with the longitudinal moving assembly, an ELISA plate is placed in the middle part above the bottom plate, and the ELISA plate is positioned below the longitudinal moving assembly;

the longitudinal movement subassembly include with two second backup pads of lateral shifting subassembly rigid coupling, two rotate the both ends that are connected with two-way lead screw between the second backup pad lateral wall, two-way lead screw one end coupling has the output shaft of second motor, the second motor lateral wall with second backup pad lateral wall rigid coupling, two-way lead screw threaded connection has the liquid feeding portion that two symmetries set up, liquid feeding portion links to each other with the imbibition subassembly, two the rigid coupling has two slide rails, two between the second backup pad lateral wall the slide rail is located two-way lead screw both sides, the slide rail with liquid feeding portion sliding connection.

Preferably, liquid feeding portion includes the second slider, the second slider lateral wall with slide rail sliding connection, second slider middle part rigid coupling has lead screw nut, lead screw nut with two-way lead screw threaded connection is located the difference on the second slider lead screw nut's screw thread revolves to opposite, second slider bottom rigid coupling has the liquid feeding head, the inside through-hole of having seted up of liquid feeding head, the through-hole with liquid feeding head bottom and lateral wall intercommunication.

Preferably, the lateral shifting subassembly includes two first lead screws, first lead screw both ends respectively with relative first backup pad rotates to be connected, first lead screw threaded connection has first slider, first slider top with second backup pad rigid coupling, two the tip of first lead screw homonymy has the coupling to take turns, two the synchronizing wheel has cup jointed the both ends of hold-in range, one of which the tip of first lead screw is provided with power portion.

Preferably, the power part comprises a gearwheel, the gearwheel is in shaft joint with the first lead screw, a pinion is connected with the gearwheel in a meshed manner, the pinion is in shaft joint with an output shaft of a first motor, a cushion block is fixedly connected to the bottom of the first motor, and the bottom of the cushion block is fixedly connected with the bottom plate.

Preferably, the imbibition subassembly includes reagent bottle and spacing seat, spacing seat bottom with bottom plate upper surface rigid coupling, the reagent bottle is placed inside the spacing seat, the reagent bottle has two peristaltic pump's inlet end through the pipe intercommunication respectively, peristaltic pump bottom with bottom plate upper surface rigid coupling, the peristaltic pump's outlet end pass through the pipe with the through-hole intercommunication.

Preferably, the side wall of the second sliding block is detachably connected with a wire clamp through a screw, and the wire clamp is used for fixing the guide pipe.

Preferably, a waste liquid groove is arranged on the upper surface of the bottom plate and is positioned between the enzyme labeling plate and the first supporting plate.

Preferably, a limiting plate is fixedly connected to the middle of the upper surface of the bottom plate, the limiting plate is in an Contraband-shaped structure, and the inner surface of the limiting plate is in contact with the elisa plate.

The utility model has the following technical effects: the transverse moving assembly and the longitudinal moving assembly are matched with each other to control the movement of the liquid adding part X, Y, so that the liquid adding part can sweep the whole enzyme label plate area to complete the liquid adding function; the two first supporting plates are used for supporting the transverse moving assembly and the longitudinal moving assembly, the two second supporting plates are used for supporting the bidirectional screw rod and are used for installing the two slide rails at the same time, the two symmetrically-arranged liquid adding parts in threaded connection with the bidirectional screw rod are used for adding liquid into the enzyme label plate, the two liquid adding parts are used for adding liquid at the same time, and the liquid adding efficiency is doubled compared with that of the prior art with a single liquid adding part; the second motor drives the two-way screw rod to rotate, the two-way screw rod drives the two liquid adding portions to move in opposite directions or in a back-to-back direction, liquid is added when the liquid adding portions are located right above the hole positions of the ELISA plate, the two liquid adding portions have flexible walking modes, and the two liquid adding portions are adjusted according to the requirements of detection tests.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without inventive exercise.

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view of a longitudinal motion assembly according to the present invention;

FIG. 3 is a schematic view of a liquid filling head structure;

FIG. 4 is a schematic view of the structure of the bottom plate, the limiting seat and the limiting plate of the present invention;

wherein, 1, a bottom plate; 2. a first support plate; 3. a first lead screw; 4. a synchronizing wheel; 5. a synchronous belt; 6. a bull gear; 7. a pinion gear; 8. a first motor; 9. cushion blocks; 10. a first slider; 11. a second support plate; 12. a second motor; 13. a bidirectional lead screw; 14. a lead screw nut; 15. a second slider; 16. a line card; 17. a liquid adding head; 18. a slide rail; 19. a peristaltic pump; 20. a limiting seat; 21. a reagent bottle; 22. a waste liquid tank; 23. a limiting plate; 24. an ELISA plate; 25. and a through hole.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.

Referring to fig. 1-4, the embodiment provides an enzyme-linked immunosorbent assay sample adding device for detecting lipoprotein phospholipase a2, which comprises a bottom plate 1, wherein two first support plates 2 are symmetrically and fixedly connected above the bottom plate 1, a transverse moving assembly is arranged between the two first support plates 2, the transverse moving assembly is fixedly connected with a longitudinal moving assembly, a liquid suction assembly is arranged at the edge part above the bottom plate 1, the liquid suction assembly is connected with the longitudinal moving assembly, an elisa plate 24 is placed at the middle part above the bottom plate 1, and the elisa plate 24 is positioned below the longitudinal moving assembly;

the longitudinal movement subassembly includes two second backup pads 11 with the rigid coupling of lateral movement subassembly, rotate the both ends that are connected with two-way lead screw 13 between 11 lateral walls of two second backup pads, two-way lead screw 13 one end coupling has the output shaft of second motor 12, second motor 12 lateral wall and 11 lateral wall rigid couplings of second backup pad, two-way lead screw 13 threaded connection has the liquid feeding portion that two symmetries set up, liquid feeding portion links to each other with the imbibition subassembly, the rigid coupling has two slide rails 18 between 11 lateral walls of two second backup pads, two slide rails 18 are located two-way lead screw 13 both sides, slide rail 18 and liquid feeding portion sliding connection.

The transverse moving assembly and the longitudinal moving assembly are matched with each other to control the movement of the liquid adding part X, Y, so that the liquid adding part can sweep the whole area of the ELISA plate 24 to complete the liquid adding function; the two first supporting plates 2 are used for supporting the transverse moving assembly and the longitudinal moving assembly, the two second supporting plates 11 are used for supporting the two-way screw 13 and are used for installing the two slide rails 18, the two symmetrically-arranged liquid adding parts in threaded connection with the two-way screw 13 are used for adding liquid into the ELISA plate 24, the two liquid adding parts are used for adding liquid simultaneously, and the liquid adding efficiency is doubled compared with that of the prior art with a single liquid adding part; the second motor 12 drives the two-way screw 13 to rotate, the two-way screw 13 drives the two liquid adding portions to move in opposite directions or in a back-to-back direction, liquid is added when the liquid adding portions are located right above the hole positions of the ELISA plate 24, and meanwhile the two liquid adding portions have flexible walking modes and are adjusted according to the requirements of detection tests.

Further optimize the scheme, liquid feeding portion includes

second slider

15, 15 lateral walls of second slider and 18 sliding connection of slide rail, and 15 middle part rigid couplings of second slider have screw nut 14, screw nut 14 and two-way lead screw 13 threaded connection, and the screw thread that is located screw nut 14 on different second sliders 15 revolves to the opposite direction, and 15 bottom rigid couplings of second slider have liquid feeding head 17, and the inside through-hole of having seted up of liquid feeding head 17, through-hole and liquid feeding head 17 bottom and lateral wall intercommunication. The screw nut 14 is matched with the bidirectional screw 13, the bidirectional screw 13 drives the screw nut 14 to do linear motion when rotating, the two screw nuts 14 are close to or far away from each other, and the liquid adding head 17 at the bottom of the second sliding block 15 is used for penetrating through a guide pipe to add liquid into the ELISA plate 24.

Further optimize the scheme, the lateral shifting subassembly includes two

first lead screws

3, and 3 both ends of first lead screw rotate with relative first backup pad 2 respectively and are connected, and 3 threaded connection of first lead screw has first slider 10, first slider 10 top and second backup pad 11 rigid coupling, and the tip of two first lead screws 3 homonymies has hub connection respectively synchronizing wheel 4, and two synchronizing wheels 4 have cup jointed the both ends of hold-in range 5, and the tip of one of them first lead screw 3 is provided with power portion. The rotation direction of two first lead screws 3 is the same, and first lead screw 3 drives first slider 10 and is linear motion when rotating, and two first sliders 10 drive second backup pad 11 and are linear motion, and the limit portion coupling of two first lead screws 3 has synchronizing wheel 4, and two synchronizing wheels 4 are connected by 5 transmission of hold-in range, guarantee that the rotation of two first lead screws 3 is synchronous, prevent the emergence of the dead condition of second backup pad 11 card.

According to a further optimized scheme, the power part comprises a large gear 6, the large gear 6 is in shaft connection with the first lead screw 3, the large gear 6 is in meshed connection with a small gear 7, the small gear 7 is in shaft connection with an output shaft of a first motor 8, a cushion block 9 is fixedly connected to the bottom of the first motor 8, and the bottom of the cushion block 9 is fixedly connected with the bottom plate 1. The rotation of first motor 8 drives the rotation of pinion 7, and pinion 7 drives gear wheel 6 and rotates, and gear wheel 6 drives first lead screw 3 and rotates, and step motor is preferred to first motor 8, and control accuracy is high, promotes the precision of walking of liquid feeding head 17.

Further optimizing scheme, imbibition subassembly includes reagent bottle 21 and spacing seat 20, and spacing seat 20 bottom and bottom plate 1 upper surface rigid coupling, reagent bottle 21 are placed inside spacing seat 20, and reagent bottle 21 communicates the entrance point that has two peristaltic pumps 19 respectively through the pipe, and peristaltic pump 19 bottom and bottom plate 1 upper surface rigid coupling, and the exit end of peristaltic pump 19 communicates with the through-hole through the pipe. The limiting seat 20 prevents the reagent bottle 21 from turning over, the peristaltic pumps 19 are used for sucking liquid into the reagent bottle 21, conveying the liquid to the outlet of the conduit, and dripping the liquid into the ELISA plate 24, the two peristaltic pumps 19 are respectively communicated with the two conduits, and the two conduits are respectively connected with the two liquid adding heads 17.

In a further optimized scheme, a line card 16 is detachably connected to the side wall of the second sliding block 15 through a screw, and the line card 16 is used for fixing a conduit. The line card 16 prevents the conduit from affecting the movement of the first slider 10 and the second slider 15.

In a further optimized scheme, a waste liquid groove 22 is arranged on the upper surface of the bottom plate 1, and the waste liquid groove 22 is positioned between the ELISA plate 24 and the first supporting plate 2. Before liquid is added, the two liquid adding heads 17 are moved to the position above the waste liquid groove 22 to remove air in the guide pipe, so that the whole guide pipe is filled with liquid and then moved to the position above the ELISA plate 24, and then liquid adding operation is started, so that the liquid in the two guide pipes can be simultaneously removed during liquid adding, and the situations that one guide pipe discharges liquid and the other guide pipe discharges air are prevented.

According to the further optimized scheme, the middle part of the upper surface of the bottom plate 1 is fixedly connected with a limiting plate 23, the limiting plate 23 is of an Contraband-shaped structure, and the inner surface of the limiting plate 23 is in contact with the ELISA plate 24. The position of the ELISA plate 24 is limited by the limiting plate 23, and liquid is prevented from dripping outside the hole position of the ELISA plate 24.

The first motor 8, the second motor 12 and the peristaltic pump 19 are all electrically connected to a controller, the controller may be a PLC controller or the like, and the controller is prior art and will not be described herein.

The working process of the embodiment is as follows: placing an ELISA plate 24 in the middle of a limiting plate 23, enabling the end face to be tightly attached to the limiting plate 23, inserting a conduit at the inlet end of a peristaltic pump 19 into a reagent bottle 21, controlling a first motor 8 to rotate through a controller, moving a longitudinal moving assembly to the upper side of a waste liquid groove 22, controlling the two peristaltic pumps 19 to start through the controller, controlling the first motor 8 to rotate through the controller after the two conduits remove liquid, moving the longitudinal moving assembly to a start position, and then performing liquid adding operation; the controller controls the first motor 8 to rotate, the first motor 8 drives the big gear 6 to rotate through the small gear 7, the big gear 6 drives the first lead screw 3 to rotate, meanwhile, a first lead screw 3 drives another first lead screw 3 to rotate through a synchronous wheel 4 and a synchronous belt 5, the two first lead screws 3 jointly drive a first slide block 10 to move to the position above a row of outermost holes of an ELISA plate 24, a controller controls a second motor 12 to rotate, the second motor 12 drives a bidirectional lead screw 13 to rotate, two lead screw nuts 14 drive a liquid adding head 17 to move from the outermost holes to the innermost holes through a second slide block 15, the liquid adding head stops moving to the position right above the holes, a peristaltic pump 19 rotates for a circle to add liquid to the holes, and after the liquid adding of one hole is finished, the liquid adding head 17 moves to the next hole, and the liquid adding operation is circulated in this way until all the hole sites on the whole ELISA plate 24 are completely added with liquid.

In the description of the present invention, it is to be understood that the terms "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, are merely for convenience of description of the present invention, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.

The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.

Claims

1. An enzyme-linked immunosorbent assay sample adding device for detecting lipoprotein phospholipase A2, which is characterized in that: the enzyme-labeled plate is characterized by comprising a bottom plate (1), wherein two first supporting plates (2) are symmetrically and fixedly connected above the bottom plate (1), a transverse moving assembly is arranged between the two first supporting plates (2), the transverse moving assembly is fixedly connected with a longitudinal moving assembly, a liquid suction assembly is arranged on the upper side part of the bottom plate (1), the liquid suction assembly is connected with the longitudinal moving assembly, an enzyme-labeled plate (24) is placed in the middle part above the bottom plate (1), and the enzyme-labeled plate (24) is positioned below the longitudinal moving assembly;

longitudinal movement subassembly include with two second backup pads (11) of lateral shifting subassembly rigid coupling, two rotate between second backup pad (11) lateral wall and be connected with the both ends of two-way lead screw (13), two-way lead screw (13) one end coupling has the output shaft of second motor (12), second motor (12) lateral wall with second backup pad (11) lateral wall rigid coupling, two-way lead screw (13) threaded connection has the liquid feeding portion that two symmetries set up, liquid feeding portion links to each other with the imbibition subassembly, two the rigid coupling has two slide rails (18), two between second backup pad (11) lateral wall slide rail (18) are located two-way lead screw (13) both sides, slide rail (18) with liquid feeding portion sliding connection.

2. The ELISA sample application device for detecting lipoprotein phospholipase A2 as claimed in claim 1, wherein: liquid feeding portion includes second slider (15), second slider (15) lateral wall with slide rail (18) sliding connection, second slider (15) middle part rigid coupling has screw nut (14), screw nut (14) with two-way lead screw (13) threaded connection is located the difference on second slider (15) the screw thread of screw nut (14) is revolved to opposite, second slider (15) bottom rigid coupling has liquid feeding head (17), liquid feeding head (17) inside has seted up the through-hole, the through-hole with liquid feeding head (17) bottom and lateral wall intercommunication.

3. The ELISA sample application device for detecting lipoprotein phospholipase A2 as claimed in claim 2, wherein: the lateral shifting subassembly includes two first lead screws (3), first lead screw (3) both ends respectively with relative first backup pad (2) rotate and connect, first lead screw (3) threaded connection has first slider (10), first slider (10) top with second backup pad (11) rigid coupling, two the tip of first lead screw (3) homonymy has hub connection synchronizing wheel (4), two synchronizing wheel (4) have cup jointed the both ends of hold-in range (5), one of them the tip of first lead screw (3) is provided with power portion.

4. The ELISA sample application device for detecting lipoprotein phospholipase A2 as recited in claim 3, wherein: the power portion includes gear wheel (6), gear wheel (6) with first lead screw (3) coupling, gear wheel (6) meshing is connected with pinion (7), pinion (7) coupling has the output shaft of first motor (8), first motor (8) bottom rigid coupling has cushion (9), cushion (9) bottom with bottom plate (1) rigid coupling.

5. The ELISA sample application device for detecting lipoprotein phospholipase A2 as claimed in claim 2, wherein: the imbibition subassembly includes reagent bottle (21) and spacing seat (20), spacing seat (20) bottom with bottom plate (1) upper surface rigid coupling, reagent bottle (21) are placed inside spacing seat (20), reagent bottle (21) communicate respectively through the pipe has the entrance point of two peristaltic pumps (19), peristaltic pump (19) bottom with bottom plate (1) upper surface rigid coupling, the exit end of peristaltic pump (19) pass through the pipe with the through-hole intercommunication.

6. The ELISA sample application device for detecting lipoprotein phospholipase A2 as recited in claim 5, wherein: the side wall of the second sliding block (15) is detachably connected with a line card (16) through a screw, and the line card (16) is used for fixing a guide pipe.

7. The ELISA sample application device for detecting lipoprotein phospholipase A2 as claimed in claim 1, wherein: and a waste liquid groove (22) is arranged on the upper surface of the bottom plate (1), and the waste liquid groove (22) is positioned between the ELISA plate (24) and the first supporting plate (2).

8. The ELISA sample application device for detecting lipoprotein phospholipase A2 as claimed in claim 1, wherein: the middle part of the upper surface of the bottom plate (1) is fixedly connected with a limiting plate (23), the limiting plate (23) is of an Contraband-shaped structure, and the inner surface of the limiting plate (23) is in contact with the ELISA plate (24).