CN216039610U - PCR fluorescence detection temperature rising and falling module and temperature rising and falling system - Google Patents
PCR fluorescence detection temperature rising and falling module and temperature rising and falling system Download PDFInfo
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- CN216039610U CN216039610U CN202122698351.2U CN202122698351U CN216039610U CN 216039610 U CN216039610 U CN 216039610U CN 202122698351 U CN202122698351 U CN 202122698351U CN 216039610 U CN216039610 U CN 216039610U
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Abstract
The utility model discloses a PCR fluorescence detection temperature rising and falling module, which comprises a bottom plate and a reagent tube seat assembly; the reagent tube seat assembly comprises a reagent tube seat, the top surface of the reagent tube seat is provided with a reaction seat, a reaction cavity is arranged in the reaction seat, and the bottom surface of the reagent tube seat is provided with a temperature control assembly for controlling the temperature rise and fall circulation in the reaction cavity; and the bottom plate is provided with a pressing assembly for pressing and fixing the reagent tube seat. The utility model also discloses a PCR fluorescence detection temperature rising and falling system which comprises the PCR fluorescence detection temperature rising and falling module and a heat dissipation assembly, wherein the heat dissipation assembly comprises a middle air duct, two sides of the middle air duct are respectively provided with a side air duct communicated with the middle air duct, the two side air ducts are respectively provided with the PCR fluorescence detection temperature rising and falling module, and the bottom plate is arranged on the corresponding side air duct.
Description
Technical Field
The utility model belongs to the technical field of medical instruments, and particularly relates to a PCR fluorescence detection temperature rising and falling module and a temperature rising and falling system.
Background
The fluorescence analysis method is a method for performing qualitative or quantitative analysis by utilizing fluorescence which is generated by de-excitation process of collision and emission of excited molecules and can reflect the characteristics of certain substances when the substances are in an excited state after being irradiated by ultraviolet light. Since some substances do not emit fluorescence by themselves (or fluoresce very weakly), it is necessary to convert the non-fluorescent substance into a substance that emits fluorescence. For example, with certain reagents (e.g., fluorescent dyes) that form complexes with non-fluorescent materials, each complex being capable of emitting fluorescence, and the assay is performed. Therefore, the use of the fluorescent reagent opens the door to the fluorescence analysis of some inorganic substances and organic substances which do not originally fluoresce, and expands the analysis range.
The fluorescence quantitative analyzer is a reaction instrument for real-time detection, and the functions of the instrument are generally ensured by a heat circulation system and a fluorescence real-time detection system. The fluorescence quantitative analyzer mainly comprises a temperature control module, a fluorescence detection module, a circuit control module, a computer and processing software thereof. The existing temperature control module realizes the temperature cycle of PCR through air bath, water bath, metal bath and other forms, and although the temperature control module can meet the requirement of temperature rise and fall cycle to a certain extent, the temperature control module has the defects of large volume and low temperature rise and fall speed.
Disclosure of Invention
In view of the above, the present invention provides a PCR fluorescence detection temperature increasing and decreasing module and a temperature increasing and decreasing system, which can realize temperature increasing and decreasing cycle control of PCR.
In order to achieve the purpose, the utility model provides the following technical scheme:
the utility model firstly provides a PCR fluorescence detection temperature rising and falling module, which comprises a bottom plate and a reagent tube seat assembly;
the reagent tube seat assembly comprises a reagent tube seat, the top surface of the reagent tube seat is provided with a reaction seat, a reaction cavity is arranged in the reaction seat, and the bottom surface of the reagent tube seat is provided with a temperature control assembly for controlling the temperature rise and fall circulation in the reaction cavity;
and the bottom plate is provided with a pressing assembly for pressing and fixing the reagent tube seat.
Furthermore, an optical fiber connecting seat is arranged on the outer side wall of the reaction seat, two connecting ports which are respectively used for being connected with optical fibers are arranged on the optical fiber connecting seat, and the connecting ports are communicated with the reaction cavity.
Furthermore, step surfaces are respectively arranged on two sides of the reagent tube seat, the pressing assembly comprises tube seat pressing plates respectively positioned on two sides of the reagent tube seat, and the tube seat pressing plates are fixedly connected with the bottom plate and pressed on the corresponding step surfaces.
Furthermore, an optical fiber fixing plate is arranged on the bottom plate, a fixing plate limiting groove used for limiting the optical fiber is formed in the optical fiber fixing plate, and an optical fiber pressing plate used for pressing and fixing the optical fiber in the fixing plate limiting groove is installed on the optical fiber fixing plate.
Furthermore, a pressing plate limiting groove for limiting the optical fiber is arranged on the tube seat pressing plate between the optical fiber fixing plate and the reagent tube seat, the pressing plate limiting groove is arranged corresponding to the fixing plate limiting groove, and the corresponding pressing plate limiting groove and the fixing plate limiting groove are displaced on the same straight line.
Furthermore, a plurality of reaction seats are arranged on the top surface of the reagent tube seat at intervals, and the reaction seats are arranged on the top surface of the reagent tube seat in a one-dimensional linear array; and a thermistor is arranged in the reagent tube seat and is positioned between two adjacent reaction seats.
Further, the reagent tube seat temperature control device further comprises an over-temperature protection switch for preventing the reagent tube seat temperature from being too high.
Furthermore, the temperature control assembly comprises a temperature control element for controlling temperature rise and fall, and a first heat conduction layer and a second heat conduction layer are respectively arranged on the upper side surface and the lower side surface of the temperature control element; the temperature control element adopts a semiconductor refrigerator; the first heat conduction layer and the second heat conduction layer are both made of graphene.
The utility model also provides a PCR fluorescence detection temperature rising and falling system which comprises the PCR fluorescence detection temperature rising and falling module and a heat dissipation assembly, wherein the heat dissipation assembly comprises a middle air duct, two sides of the middle air duct are respectively provided with a side air duct communicated with the middle air duct, the two side air ducts are respectively provided with the PCR fluorescence detection temperature rising and falling module, and the bottom plate is arranged on the corresponding side air duct.
Furthermore, communicating holes communicated with the two side air channels are respectively formed in two side walls of the middle air channel, and a partition plate located between the two communicating holes is arranged in the middle air channel.
The utility model has the beneficial effects that:
according to the PCR fluorescence detection heating and cooling module, the reagent tube seat assembly is arranged on the bottom plate, the reaction seat is arranged on the top surface of the reagent tube seat, and the reaction cavity is arranged in the reaction seat; meanwhile, the temperature control component is utilized to control the temperature rise and fall circulation of the reaction cavity, so that the temperature rise and fall circulation control of the PCR can be realized.
Through setting up the fiber connection seat to set up two connectors on the fiber connection seat, the optic fibre of connecting is respectively for arousing optic fibre and receiving optical fibre on two connectors, thereby can improve the connection stability and the connection uniformity of optic fibre, avoids optic fibre to drop.
The temperature is detected by the thermistor, the thermistor is arranged between the two adjacent reaction seats, namely the thermistor avoids the positions of the reaction seats, so that the influence of heat conduction between the reagent tube seat and the reaction seats, caused by the existence of the thermistor, is reduced, the heat transfer efficiency is improved, and the temperature uniformity of each reaction seat is improved.
Through setting up temperature control element control temperature and going up and down to set up first heat-conducting layer and second heat-conducting layer respectively in temperature control element's upper and lower both sides, so, can realize the quick heat-conduction between temperature element and the reagent tube socket through first heat-conducting layer, and when needs cooling down, accessible second heat-conducting layer exports the heat, improves cooling rate.
Through set up the optic fibre fixed plate on the bottom plate to set up the fixed plate spacing groove on the optic fibre fixed plate, optic fibre carries on spacingly through this fixed plate spacing groove, thereby can make optic fibre arrange more regular, adopts the optic fibre clamp plate to compress tightly optic fibre simultaneously and fixes at the fixed plate spacing inslot, realizes compressing tightly of optic fibre fixedly.
According to the PCR fluorescence detection temperature rising and falling system, the PCR fluorescence detection temperature rising and falling module is arranged on the side air duct, so that when the temperature control assembly cools the reagent tube seat, heat generated at the bottom can be rapidly taken away through the convection effect of the side air duct, and the cooling efficiency is improved.
Through setting up the baffle, can prevent to influence each other between two side wind channels.
Drawings
In order to make the object, technical scheme and beneficial effect of the utility model more clear, the utility model provides the following drawings for explanation:
FIG. 1 is a schematic structural diagram of an embodiment of a full-automatic fluorescence quantitative analyzer employing the PCR fluorescence detection temperature raising and lowering system of the present invention, specifically an assembly diagram of a temperature raising and lowering module and a heat dissipation assembly;
FIG. 2 is a schematic structural diagram of a PCR fluorescence detection temperature raising and lowering module;
FIG. 3 is a cross-sectional view of a PCR fluorescence detection temperature raising and lowering module;
FIG. 4 is a schematic view of a reagent tube holder;
FIG. 5 is a schematic view of a thermal lid assembly;
FIG. 6 is a schematic structural diagram of a fluorescence detection module;
FIG. 7 is a top view of FIG. 6;
FIG. 8 is a schematic view of the structure of the test tray;
FIG. 9 is a schematic structural diagram of an excitation light assembly;
fig. 10 is a schematic structural diagram of a light receiving module.
Detailed Description
The present invention is further described with reference to the following drawings and specific examples so that those skilled in the art can better understand the present invention and can practice the present invention, but the examples are not intended to limit the present invention.
FIG. 1 is a schematic structural diagram of an embodiment of the full-automatic fluorescence quantitative analyzer of the present invention. The full-automatic fluorescence quantitative analyzer comprises a temperature control system and a fluorescence detection module, wherein the temperature control system comprises a PCR fluorescence detection temperature rise and fall system and a hot cover component.
Specifically, as shown in fig. 2 and 3, the PCR fluorescence detection temperature increasing and decreasing system includes a PCR fluorescence detection temperature increasing and decreasing module (hereinafter referred to as "temperature increasing and decreasing module") and a heat dissipation assembly. The temperature raising and lowering module includes a base plate 100 and a reagent tube holder assembly. The reagent tube seat assembly of this embodiment includes reagent tube seat 101, and the top surface of reagent tube seat 101 is equipped with reaction seat 102, is equipped with reaction chamber 103 in the reaction seat 102, and the bottom surface of reagent tube seat 101 is equipped with the temperature control assembly who is used for controlling the temperature lift circulation in the reaction chamber 103. The bottom plate 100 is provided with a pressing assembly for pressing and fixing the reagent tube holder 101. The reagent well base 101 of this embodiment has a plurality of reaction wells 102 spaced apart from each other on the top surface thereof, and all the reaction wells 102 are arranged in a one-dimensional linear array on the top surface of the reagent well base 102. The top surface of the reagent well base 101 of this embodiment is provided with 8 reaction wells 102 arranged in a one-dimensional linear array at intervals.
As shown in fig. 5, the heat cover assembly includes a fixing plate 200 and a heat cover 201 that can be covered on the fixing plate 200; a reaction zone 202 is arranged on the fixing plate 200, and a reaction through hole 203 for the reaction seat 102 to pass through is arranged in the reaction zone 202; the heating device 204 is disposed on the thermal cover 201 corresponding to the reaction area 202. The fixing plate 200 is located above the bottom plate 100, and the fixing plate 200 and the bottom plate 100 are relatively fixed; the reaction through holes 203 are arranged corresponding to the reaction sockets 102 one by one, and the reaction sockets 102 are located in the corresponding reaction through holes 203.
As shown in FIG. 6, the fluorescence detection module includes an interface disc 301, a detection disc 302, and a drive mechanism. The interface disc 301 is provided with a plurality of interface groups, and each interface group comprises an excitation optical fiber interface 303 and a receiving optical fiber interface 304. The detection plate 302 is provided with a plurality of detection elements, including an excitation light element 305 and a receiving light element 306. The driving mechanism drives the interface disc 301 and the detection disc 302 to move relatively, so that each interface group passes through all the detection assemblies in sequence, or each detection assembly passes through all the interface groups in sequence. Specifically, the following alignment relationship is satisfied between the interface group and the detection component: when the excitation light assembly 305 of the detection assembly is aligned with the excitation light interface 303 of the interface set, the receiving light assembly 306 of the detection assembly is aligned with the receiving optical fiber interface 304 of the interface set; similarly, when the receiving optical element 306 of the detection element is aligned with the receiving optical fiber interface 304 of the interface set, the excitation optical element 305 of the detection element is aligned with the excitation optical interface 303 of the interface set. In this way, in the aligned interface set and detection assembly, the light emitted from the excitation light assembly 305 is transmitted into the optical fiber 106 connected to the excitation light interface 303 corresponding thereto, and the fluorescence generated by the reagent in the reagent tube placed in the reaction chamber 103 is transmitted into the receiving light set 306 through the optical fiber 106 connected to the interface light interface 304.
An optical fiber connecting seat 104 is arranged on the outer side wall of the reaction seat 102, two connecting ports 105 are arranged on the optical fiber connecting seat 104, and the connecting ports 105 are communicated with the reaction cavity 103; the interface group is arranged corresponding to the reaction seat 102, and the two connecting ports 105 of the reaction seat 102 are respectively connected with the excitation optical fiber interface 303 and the receiving optical fiber interface 304 of the corresponding interface group through optical fibers 106.
Further, two sides of the reagent tube seat 101 are respectively provided with a step surface 107, the compressing assembly comprises tube seat pressing plates 108 respectively positioned at two sides of the reagent tube seat 101, and the tube seat pressing plates 108 are fixedly connected with the bottom plate 100 and pressed on the corresponding step surfaces 107. By providing the step surface 107, the reagent well block 101 can be firmly pressed and fixed to the bottom plate 100 by the well block pressing plate 108, and the reagent well block 101 does not need to be provided with a connecting member such as a screw connector, and the heat transfer thereof is not affected.
Further, an optical fiber fixing plate 109 is disposed on the bottom plate 100, a fixing plate limiting groove 110 for limiting the optical fiber 106 is disposed on the optical fiber fixing plate 109, and an optical fiber pressing plate 111 for pressing and fixing the optical fiber 106 in the fixing plate limiting groove 110 is disposed on the optical fiber fixing plate 109. Through set up fiber fixing plate 109 on bottom plate 100, and set up fixed plate spacing groove 110 on fiber fixing plate 109, optic fibre 106 is spacing through this fixed plate spacing groove 110, thereby can make optic fibre 106 arrange more regular, adopt optic fibre clamp plate 111 to compress tightly optic fibre 106 and fix in fixed plate spacing groove 110 simultaneously, realize compressing tightly of optic fibre 106 fixed, simultaneously can also control the bending radius of optic fibre 106 between fiber fixing plate 109 and the reaction seat 102 that corresponds, make optic fibre 106 receive the power along connector 105 axis direction, this effort can prevent to a certain extent that optic fibre 106 from producing axial displacement along connector 105. Preferably, the bottom surface of the optical fiber pressing plate 111 of the present embodiment is provided with an optical fiber cushion 112, which can not only press and fix the optical fiber 106, but also avoid damage to the optical fiber 106.
Furthermore, a tube seat pressing plate 108 located between the optical fiber fixing plate 109 and the reagent tube seat 101 is provided with a pressing plate limiting groove 113 for limiting the optical fiber 106, the pressing plate limiting groove 113 is arranged corresponding to the fixing plate limiting groove 110, and the corresponding pressing plate limiting groove 113 and the fixing plate limiting groove 110 are displaced on the same straight line, so as to further limit the bending radius of the optical fiber 106, so that the optical fiber 106 is subjected to a larger force along the axial direction of the connecting port 105.
Further, a plurality of reaction seats 102 are arranged on the top surface of the reagent tube seat 101 at intervals, and the reaction seats 102 of the present embodiment are arranged on the top surface of the reagent tube seat 101 in a one-dimensional linear array; the thermistor 114 is arranged in the reagent tube seat 101, and the thermistor 114 is positioned between two adjacent reaction seats 102. The thermistor 114 is arranged to detect the temperature, and the thermistor 114 is arranged between two adjacent reaction seats, namely, the thermistor 114 avoids the position of the reaction seat 102, so that the influence of the existence of the thermistor 114 on the heat conduction between the reagent tube seat 101 and the reaction seat 102 is reduced, the heat transfer efficiency is improved, and the temperature uniformity of each reaction seat 102 is improved.
Further, the module of heating and cooling of this embodiment still includes the excess temperature protection switch that is used for preventing reagent tube seat 101 high temperature, carries out excess temperature protection to the module of heating and cooling, avoids the high temperature.
Further, the temperature control assembly of the present embodiment includes a temperature control element 115 for controlling temperature rise and fall, and a first heat conduction layer 116 and a second heat conduction layer 117 are respectively disposed on the upper and lower sides of the temperature control element 115. Specifically, the temperature control element 115 of the present embodiment employs a semiconductor refrigerator; the first heat conducting layer 116 and the second heat conducting layer 117 are made of graphene. Temperature rise and fall are controlled by arranging the temperature control element 115, and the upper side and the lower side of the temperature control element 115 are respectively provided with the first heat conduction layer 116 and the second heat conduction layer 117, so that the rapid heat conduction between the temperature control element 115 and the reagent tube seat 101 can be realized through the first heat conduction layer 116, and when the temperature needs to be reduced, the heat can be led out through the second heat conduction layer 117, and the temperature reduction rate is improved.
Further, the periphery of the reaction area 202 is provided with heat insulation boards 205 for contacting and matching with the heating device 204, so as to insulate the heat of the temperature raising and lowering module, and facilitate the temperature control in the reaction area 202.
Further, a heating installation groove for installing the heating device 204 is formed in the thermal cover 201, the heating device is installed in the heating installation groove, a flexible elastic element is arranged between the heating device 204 and the bottom of the heating installation groove, the flexible elastic element exerts flexible elastic force on the heating device 204, the thermal cover 201 can be smoothly covered on the fixing plate 200, meanwhile, the heating device 204 can be pressed on the reaction seat 102 and the reagent tube, and heating efficiency is improved.
Further, the bottom surface of the fixing plate 200, which faces away from the hot cover 201, is provided with heat insulation cotton 206, and the heat insulation cotton 206 is provided with heat insulation through holes for the reaction seat 102 to pass through in one-to-one correspondence with the reaction through holes 203, so that the reaction seat 102 can be insulated.
Further, the connection end of the thermal cover 201 is hinged to the fixing plate 200, and a lock catch 207 is disposed between the opening/closing end of the thermal cover 201 opposite to the connection end and the fixing plate 200, so that the thermal cover 201 can be locked after the thermal cover 201 is closed. Preferably, the opening and closing end of the thermal cover 201 and/or the end of the fixing plate 200 provided with the latch 207 are provided with an electromagnet 208, and specifically, the electromagnet 208 of the present embodiment is provided on the fixing plate 200. By providing the electromagnet 208, the electromagnet 208 is electrified during operation, and a magnetic force is generated between the thermal cover 201 and the fixing plate 200 to ensure that the thermal cover 201 cannot be opened during operation.
The PCR fluorescence detection temperature rising and falling system of this embodiment still includes radiator unit, and radiator unit includes middle wind channel 401, and the both sides in middle wind channel 401 are equipped with respectively rather than the side wind channel 402 that is linked together, are equipped with respectively on two side wind channels 402 and rise temperature module and hot lid subassembly, and fixed plate 200 and bottom plate 100 are all fixed mounting in the correspondence on the side wind channel 402. In this embodiment, two side walls of the middle air duct 401 are respectively provided with communication holes 403 communicated with the two side air ducts 402, and a partition 404 located between the two communication holes 403 is provided in the middle air duct 401. Through installing on side wind channel 402 with heating and cooling module and hot cap subassembly, when temperature control assembly cooled down the reagent tube socket, the heat accessible side wind channel 402's that the bottom produced convection effect was taken away rapidly, improved cooling efficiency, through setting up baffle 404, can prevent to influence each other between two side wind channels 402.
Further, all the centers of exciting fiber optic interface 303 all are located same circular arc line I, and all the centers of receiving fiber optic interface 304 all are located a circular arc line II, and the centre of a circle coincidence of circular arc line I and circular arc line II, the radius of curvature of circular arc line I of this embodiment is greater than the radius of curvature of circular arc line II. Along the axial view direction of the excitation optical fiber interface 303 or the receiving optical fiber interface 304, the geometric centers of all the excitation optical components 305 are located on the circle on which the circular arc line i is located, and the geometric centers of all the receiving optical components 306 are located on the circle on which the circular arc line ii is located; the driving mechanism is a rotation driving mechanism for driving the interface disc 301 and the detection disc 302 to rotate relatively. The rotation driving mechanism of this embodiment includes a stepping motor 307 for driving the detection disc 302 and the interface disc 301 to rotate relatively, and the axis of the relative rotation between the detection disc 302 and the interface disc 301 passes through the centers of the circular arc line i and the circular arc line ii. The output shaft of the stepping motor 307 of the present embodiment is connected to the detection disk 302, that is, the detection disk 302 rotates synchronously with the output shaft of the stepping motor 307, and the interface disk 301 is fixedly disposed relative to the housing of the stepping motor 307. Thus, in the process of using the stepping motor 307 to drive the detection disc 302 and the interface disc 301 to rotate relatively, the excitation light assemblies 305 in all the detection assemblies sequentially pass through all the excitation optical fiber interfaces 303, the receiving light assemblies 306 sequentially pass through all the receiving optical fiber interfaces 304, each interface group corresponds to one reaction seat 102, test tubes are placed in the reaction cavities 103 of the reaction seats 102, reagents to be detected are contained in the test tubes, and thus, the same detection assembly can realize fluorescence detection of all the reagents, and the same reagent can be subjected to fluorescence detection by all the detection assemblies.
Furthermore, the included angle between the geometric centers of the excitation light assembly 305 and the receiving light assembly 306 which belong to the same detection assembly and the connecting line between the centers of the circular arc lines I is 30 degrees, and the included angle between the excitation light assembly 305 and the receiving light assembly 306 and the connecting line between the centers of the circular arc lines I is set to be 30 degrees due to the fact that the excitation light assembly 305 and the receiving light assembly 306 have certain volumes, the diameter of the detection disc 302 can be reduced, and the volume of the whole fluorescence detection module is further reduced. Specifically, the number of detection elements in this embodiment is 4, that is, the number of excitation light elements 305 and the number of reception light elements 306 are 4 respectively. The interface group of this embodiment is set to 16, and excitation fiber interface 303 and receiving fiber interface 304 are equipped with 16 respectively promptly, and correspondingly, reaction seat 102 is equipped with 16 altogether, and the module of heating and cooling is equipped with 2, is equipped with 8 reaction seats 102 on two modules of heating and cooling respectively.
Further, the fluorescence detection module of this embodiment further includes an optical coupler sensor 308 for positioning the relative position between the detection disc 302 and the interface disc 301, specifically, the optical coupler sensor 308 of this embodiment is installed on the interface disc 301, and the detection disc 302 is provided with a light blocking sheet 309 matched with the optical coupler sensor 308. The position between the interface disc 301 and the detection disc 302 is positioned by arranging the optical coupling sensor 308, so that the starting position of detection between the interface disc 301 and the detection disc 302 is determined, and the accuracy and reliability of detection are ensured. Preferably, the detection plate 302 is provided with a conductive slip ring 310, and all connection wires required by the excitation light assembly 305 and the receiving light assembly 306 are connected through the conductive slip ring 310, so that the connection wires are prevented from being wound in the rotation process of the detection plate 302.
Of course, in some embodiments, the centers of all the excitation fiber optic interfaces 303 are located on the same straight line i, the centers of all the receiving fiber optic interfaces 304 are located on a straight line ii, and the straight lines i and ii are parallel to each other; in the axial view direction of the excitation optical fiber interface 303 or the receiving optical interface 304, the geometric centers of all the excitation light assemblies 305 are located on a straight line i, and the geometric centers of all the receiving optical assemblies 306 are located on a straight line ii; the driving mechanism is a linear driving mechanism for driving the interface disc and the detection disc to move relatively. The technical purposes that the same detection assembly can realize the fluorescence detection of all reagents and the same reagent can be subjected to the fluorescence detection of all the detection assemblies can be realized, and the description is omitted.
Further, the excitation light assembly 305 includes an LED light source 311, a first convex lens 312, a second convex lens 313 and a third convex lens 314, which are sequentially disposed, a first optical filter 315 is disposed between the first convex lens 312 and the second convex lens 313, and the third convex lens 314 is located on a side of the second convex lens 313 facing the interface panel 301. The receiving optical assembly 306 of this embodiment includes a fourth convex lens 316, a fifth convex lens 317, and a PD sensor 318, which are sequentially disposed, a second optical filter 319 is disposed between the fourth convex lens 316 and the fifth convex lens 317, and the fourth convex lens 316 is located on a side of the fifth convex lens 317 facing the interface disc 301.
The above-mentioned embodiments are merely preferred embodiments for fully illustrating the present invention, and the scope of the present invention is not limited thereto. The equivalent substitution or change made by the technical personnel in the technical field on the basis of the utility model is all within the protection scope of the utility model. The protection scope of the utility model is subject to the claims.
Claims (10)
1. The utility model provides a PCR fluorescence detection goes up and down temperature module which characterized in that: comprises a bottom plate and a reagent tube seat assembly;
the reagent tube seat assembly comprises a reagent tube seat, the top surface of the reagent tube seat is provided with a reaction seat, a reaction cavity is arranged in the reaction seat, and the bottom surface of the reagent tube seat is provided with a temperature control assembly for controlling the temperature rise and fall circulation in the reaction cavity;
and the bottom plate is provided with a pressing assembly for pressing and fixing the reagent tube seat.
2. The PCR fluorescence detection cooling and heating module of claim 1, wherein: the outer side wall of the reaction seat is provided with an optical fiber connecting seat, the optical fiber connecting seat is provided with two connecting ports which are respectively used for being connected with optical fibers, and the connecting ports are communicated with the reaction cavity.
3. The PCR fluorescence detection cooling and heating module of claim 1, wherein: the reagent tube seat is characterized in that step surfaces are respectively arranged on two sides of the reagent tube seat, the pressing assembly comprises tube seat pressing plates respectively located on two sides of the reagent tube seat, and the tube seat pressing plates are fixedly connected with the bottom plate and pressed on the corresponding step surfaces.
4. The PCR fluorescence detection cooling and heating module of claim 3, wherein: the optical fiber fixing plate is characterized in that an optical fiber fixing plate is arranged on the bottom plate, a fixing plate limiting groove used for limiting optical fibers is formed in the optical fiber fixing plate, and an optical fiber pressing plate used for pressing and fixing the optical fibers in the fixing plate limiting groove is installed on the optical fiber fixing plate.
5. The PCR fluorescence detection cooling and heating module of claim 4, wherein: and a pressing plate limiting groove for limiting the optical fiber is arranged on the tube seat pressing plate between the optical fiber fixing plate and the reagent tube seat, the pressing plate limiting groove corresponds to the fixing plate limiting groove, and the corresponding pressing plate limiting groove and the fixing plate limiting groove are displaced on the same straight line.
6. The PCR fluorescence detection cooling and heating module of claim 1, wherein: the top surface of the reagent tube seat is provided with a plurality of reaction seats at intervals, and the reaction seats are arranged on the top surface of the reagent tube seat in a one-dimensional linear array; and a thermistor is arranged in the reagent tube seat and is positioned between two adjacent reaction seats.
7. The PCR fluorescence detection cooling and heating module of claim 1, wherein: the reagent tube seat temperature control device further comprises an over-temperature protection switch for preventing the reagent tube seat from being overhigh in temperature.
8. The PCR fluorescence detection cooling and heating module according to any one of claims 1-7, wherein: the temperature control component comprises a temperature control element for controlling temperature to rise and fall, and a first heat conduction layer and a second heat conduction layer are respectively arranged on the upper side surface and the lower side surface of the temperature control element; the temperature control element adopts a semiconductor refrigerator; the first heat conduction layer and the second heat conduction layer are both made of graphene.
9. A PCR fluorescence detection temperature rising and falling system is characterized in that: the PCR fluorescence detection temperature rising and lowering module comprises the PCR fluorescence detection temperature rising and lowering module and the heat dissipation assembly according to any one of claims 1-8, wherein the heat dissipation assembly comprises a middle air duct, two sides of the middle air duct are respectively provided with a side air duct communicated with the middle air duct, the two side air ducts are respectively provided with the PCR fluorescence detection temperature rising and lowering module, and the bottom plate is installed on the corresponding side air ducts.
10. The PCR fluorescence detection cooling and heating system of claim 9, wherein: the two side walls of the middle air duct are respectively provided with communicating holes communicated with the two side air ducts, and a partition plate positioned between the two communicating holes is arranged in the middle air duct.
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| CN202122698351.2U CN216039610U (en) | 2021-11-05 | 2021-11-05 | PCR fluorescence detection temperature rising and falling module and temperature rising and falling system |
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| CN202122698351.2U CN216039610U (en) | 2021-11-05 | 2021-11-05 | PCR fluorescence detection temperature rising and falling module and temperature rising and falling system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114015561A (en) * | 2021-11-05 | 2022-02-08 | 中元汇吉生物技术股份有限公司 | PCR fluorescence detection temperature control system |
| WO2023246199A1 (en) * | 2022-06-23 | 2023-12-28 | 中元汇吉生物技术股份有限公司 | Fully-automatic pcr fluorescence quantitative analyzer |
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2021
- 2021-11-05 CN CN202122698351.2U patent/CN216039610U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114015561A (en) * | 2021-11-05 | 2022-02-08 | 中元汇吉生物技术股份有限公司 | PCR fluorescence detection temperature control system |
| CN114015561B (en) * | 2021-11-05 | 2023-11-14 | 中元汇吉生物技术股份有限公司 | PCR fluorescent detection temperature control system |
| WO2023246199A1 (en) * | 2022-06-23 | 2023-12-28 | 中元汇吉生物技术股份有限公司 | Fully-automatic pcr fluorescence quantitative analyzer |
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