CN216024943U

CN216024943U - Mounting assembly

Info

Publication number: CN216024943U
Application number: CN202122004448.9U
Authority: CN
Inventors: 索海军; 黄金城; 焦少灼; 李宗文
Original assignee: Beijing Xunyin Biological Technology Co ltd
Current assignee: Beijing Xunyin Biological Technology Co ltd
Priority date: 2021-08-24
Filing date: 2021-08-24
Publication date: 2022-03-15
Anticipated expiration: 2031-08-24

Abstract

The utility model discloses an installation component, which is used for installing a microfluid chip and comprises a base and a fixing structure, wherein the upper end surface of the base is provided with an installation groove, and the installation groove is used for placing the microfluid chip; the fixed knot constructs to be located the base, fixed knot constructs including locating the portion is held in the pressure of one side of mounting groove, the portion is held in the pressure has the orientation the diapire of mounting groove is close to the activity stroke, and in its activity stroke, the portion is held in the pressure is used for supporting tightly the support plate of microfluid chip, with the microfluid chip pressure hold in on the diapire of mounting groove. According to the technical scheme, the microfluid chip is arranged on the mounting assembly, so that the microfluid chip is prevented from vibrating and moving in the transferring process, samples are protected, and the preparation accuracy of the single cell library is improved.

Description

Mounting assembly

Technical Field

The utility model relates to the technical field of single cell library preparation, in particular to an installation assembly.

Background

In recent years, with the continuous progress of sequencing technology, the high-depth single-cell sequencing is developed towards higher-throughput application. In addition to the base reads themselves, sample cDNA synthesis and library preparation are another important cost component of the overall single cell sequencing process. The existing library preparation is generally completed by a single cell library preparation system, the single cell library preparation system rapidly distinguishes single cell samples by applying a microfluidic chip, and then sequence tags are added to perform dynamic gene expression profiling on tens of thousands of single cells of the samples. The existing single cell library preparation system comprises an inlet and outlet bin, a multi-manifold plate and an air pump, wherein an air inlet of the multi-manifold plate is connected with an outlet of the air pump, the multi-manifold plate is arranged above the inlet and outlet bin, a microfluid chip is arranged in the inlet and outlet bin, the inlet and outlet bin can convey the microfluid chip to a designated position, so that an air outlet of the multi-manifold plate is over against a reagent groove of the microfluid chip, the air pump pressurizes the reagent groove of the microfluid chip through the multi-manifold plate, and in the process of transferring the microfluid chip, the microfluid chip is easy to vibrate and move, so that not only is a sample easily damaged, but also the relative positions of the reagent groove of the microfluid chip and the air outlet of the multi-manifold plate deviate, the pressurizing failure or insufficient pressurizing is caused, and the preparation accuracy of the single cell library is further reduced.

SUMMERY OF THE UTILITY MODEL

The utility model mainly aims to provide an installation assembly, and aims to solve the problem that the existing microfluid chip is easy to shake and move in the transferring process, so that the library preparation accuracy is reduced.

To achieve the above object, the present invention provides a mounting assembly for mounting a microfluidic chip, the mounting assembly comprising:

the upper end surface of the base is provided with a mounting groove, and the mounting groove is used for placing the microfluidic chip; and the number of the first and second groups,

the fixing structure is arranged on the base and comprises a pressing and holding part arranged on one side of the mounting groove, the pressing and holding part is provided with a moving stroke towards the approaching of the bottom wall of the mounting groove, and in the moving stroke, the pressing and holding part is used for abutting against the carrier plate of the microfluidic chip so as to press and hold the microfluidic chip on the bottom wall of the mounting groove.

Optionally, the fixing structure further includes a positioning portion disposed in the mounting groove, and the positioning portion is used for positioning and matching with a matching portion disposed on the microfluidic chip.

Optionally, the fixing structure includes a cover plate, the pressing portion includes a bump disposed on an end surface of the cover plate facing the base, the cover plate is rotatably mounted on the base so as to be movable to open and close a notch of the mounting groove, and when the cover plate is movable to close the notch of the mounting groove, the bump abuts against the carrier plate of the microfluidic chip;

the apron runs through along its thickness direction and is equipped with dodges the hole, dodge the hole and be used for the apron rotates to the lid and closes during the notch of mounting groove, supply microfluid chip's at least reagent groove certainly dodge the hole and show out.

Optionally, the cover plate is rotatably mounted to the base through a damping rotating shaft.

Optionally, the installation component is still including the first magnetism of mutually supporting inhale the piece and the second magnetism is inhaled the piece, first magnetism inhale the piece with the second magnetism inhale one of them and locate the base, another correspondence is located the apron, first magnetism inhale the piece with the second magnetism inhale the piece be used for the apron rotates to the lid and closes when the notch of mounting groove, mutual actuation.

Optionally, the pressing and holding part is arranged in a block shape, and the pressing and holding part is movably mounted on the side wall of the mounting groove in the vertical direction.

Optionally, the pressing part is slidably mounted on the side wall of the mounting groove in the up-down direction through a slide rail mechanism.

Optionally, the mounting assembly further comprises a sealing pad movably arranged on the base, the sealing pad is provided with a through hole along the thickness direction thereof, so that when the microfluidic chip is moved to the position below the manifold plate, the sealing pad is hermetically connected with the microfluidic chip and the manifold plate, and the through hole is communicated with the air outlet of the manifold plate and the reagent groove of the microfluidic chip.

Optionally, the fixing structure includes a cover plate rotatably mounted on the base, and the cover plate is provided with an avoidance hole along a thickness direction thereof;

the sealing gasket is arranged on one side end face of the cover plate, which deviates from the base.

Optionally, the base is provided with spacing portion, spacing portion be used for with locate the spacing cooperation of installation department in the business turn over storehouse, in order to incite somebody to action the base is fixed in business turn over storehouse.

According to the technical scheme, the microfluidic chip is arranged in the mounting groove on the base, the pressing part is arranged on one side of the mounting groove and has a movable stroke approaching to the bottom wall of the mounting groove, the pressing part moves towards the bottom wall of the mounting groove after the microfluidic chip is arranged in the mounting groove, and the pressing part is abutted against the carrier plate of the microfluidic chip, so that the microfluidic chip is pressed on the bottom wall of the mounting groove.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the structures shown in the drawings without creative efforts.

FIG. 1 is a schematic perspective view of an embodiment of a mounting assembly according to the present invention;

FIG. 2 is an assembled perspective view of the mounting assembly of FIG. 1;

fig. 3 is an exploded perspective view of the mounting assembly of fig. 2.

The embodiment of the utility model is illustrated by reference numerals:

reference numerals	Name (R)	Reference numerals	Name (R)
				100	Mounting assembly	232	Second magnetic attraction piece
1	Base seat	24	Mounting boss
				11	Mounting groove	3	Sealing gasket
12	First magnetic attraction piece	31	Through hole
				2	Fixing structure	32	Mounting hole
21	Pressing and holding part	200	Microfluidic chip
				22	Positioning part	201	Support plate
23	Cover plate	2011	Mating part
				231	Avoiding hole	202	Reagent tank

The implementation, functional features and advantages of the objects of the present invention will be further explained with reference to the accompanying drawings.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

It should be noted that, if directional indications (such as up, down, left, right, front, and back … …) are involved in the embodiment of the present invention, the directional indications are only used to explain the relative positional relationship between the components, the movement situation, and the like in a specific posture (as shown in the drawing), and if the specific posture is changed, the directional indications are changed accordingly.

In addition, if there is a description of "first", "second", etc. in an embodiment of the present invention, the description of "first", "second", etc. is for descriptive purposes only and is not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In addition, the meaning of "and/or" appearing throughout includes three juxtapositions, exemplified by "A and/or B" including either A or B or both A and B. In addition, technical solutions between various embodiments may be combined with each other, but must be realized by a person skilled in the art, and when the technical solutions are contradictory or cannot be realized, such a combination should not be considered to exist, and is not within the protection scope of the present invention.

The existing single cell library preparation system comprises an inlet and outlet bin, a multi-manifold plate and an air pump, wherein an air inlet of the multi-manifold plate is connected with an outlet of the air pump, the multi-manifold plate is arranged above the inlet and outlet bin, a microfluid chip is arranged in the inlet and outlet bin, the inlet and outlet bin can convey the microfluid chip to a designated position, so that an air outlet of the multi-manifold plate is over against a reagent groove of the microfluid chip, the air pump pressurizes the reagent groove of the microfluid chip through the multi-manifold plate, and in the process of transferring the microfluid chip, the microfluid chip is easy to vibrate and move, so that not only is a sample easily damaged, but also the relative positions of the reagent groove of the microfluid chip and the air outlet of the multi-manifold plate deviate, the pressurizing failure or insufficient pressurizing is caused, and the preparation accuracy of the single cell library is further reduced.

In view of this, the present invention provides a mounting assembly. Fig. 1 to 3 show an embodiment of a mounting assembly according to the present invention.

Referring to fig. 1, the mounting assembly 100 is used for mounting a microfluidic chip 200, the mounting assembly 100 includes a base 1 and a fixing structure 2, an installation groove 11 is formed on an upper end surface of the base 1, and the installation groove 11 is used for placing the microfluidic chip 200; the fixing structure 2 is disposed on the base 1, the fixing structure 2 includes a pressing portion 21 disposed on one side of the mounting groove 11, the pressing portion 21 has a moving stroke approaching the bottom wall of the mounting groove 11, and in the moving stroke, the pressing portion 21 is used for abutting against the carrier plate 201 of the microfluidic chip 200, so as to press and hold the microfluidic chip 200 on the bottom wall of the mounting groove 11.

In the technical scheme of the utility model, the microfluid chip 200 is arranged in the installation groove 11 on the base 1, one side of the installation groove 11 is provided with the pressing part 21, the pressing part 21 has a movable stroke approaching to the bottom wall of the installation groove 11, after the microfluid chip 200 is arranged in the installation groove 11, the pressing part 21 moves towards the bottom wall of the installation groove 11, and the pressing part 21 is pressed against the carrier plate 201 of the microfluidic chip 200, so as to press the microfluidic chip 200 on the bottom wall of the mounting groove 11, in the process of transferring the microfluidic chip 200, the pressing part 21 presses the microfluidic chip 200 on the bottom wall of the mounting groove 11, so that the microfluidic chip 200 cannot vibrate or move, the inlet and outlet chamber can convey the microfluidic chip 200 to a specified position, the air outlet of the manifold plate is opposite to the reagent groove 202 of the microfluidic chip 200, the sample is protected, and the preparation accuracy of the single-cell library is improved.

In order to ensure the accurate installation of the microfluidic chip 200, the fixing structure 2 further includes a positioning portion 22 disposed in the installation groove 11, and the positioning portion 22 is used for positioning and matching with a matching portion 2011 disposed on the microfluidic chip 200.

Specifically, the specific forms of the positioning portion 22 and the matching portion 2011 are not limited, one of the positioning portion 22 and the matching portion 2011 is entirely configured as a convex column, and the other is configured as a clamping slot, in this embodiment, the positioning portion 22 is configured as a convex column, the matching portion 2011 is configured as a clamping slot, and when the microfluidic chip 200 is disposed in the mounting groove 11, the convex column and the clamping slot are in positioning fit, so that the microfluidic chip 200 is accurately mounted.

More specifically, the mounting positions of the protruding pillars and the slots are not limited, the protruding pillars may be disposed on the side walls of the mounting grooves 11, and correspondingly, the slots are disposed on the side portions of the carrier plate 201 of the microfluidic chip 200; in this embodiment, the protruding pillar is disposed on the bottom wall of the mounting groove 11, correspondingly, the slot is disposed on one side of the carrier plate 201 of the microfluidic chip 200, which faces the protruding pillar, and along the thickness direction of the microfluidic chip 200, the sidewall of the slot is disposed in a penetrating manner, and the protruding pillar is in positioning fit with the slot, so that the microfluidic chip 200 can be mounted accurately.

More specifically, the number of the protruding columns and the number of the slots are not limited in the present invention, and may be one protruding column and one slot, or two protruding columns and two slots, in this embodiment, a plurality of protruding columns are provided at intervals along the width direction of the microfluidic chip 200, and a plurality of slots are provided correspondingly, so that the positioning of the microfluidic chip 200 is more accurate.

Further referring to fig. 1, the fixing structure 2 includes a cover plate 23, the pressing portion 21 includes a bump disposed on an end surface of the cover plate 23 facing the base 1, the cover plate 23 is rotatably mounted on the base 1 to be movable to open and close the notch of the mounting groove 11, and when the cover plate is movable to close the notch of the mounting groove 11, the bump abuts against the carrier plate 201 of the microfluidic chip 200; the cover plate 23 is provided with an avoiding hole 231 along the thickness direction thereof, the avoiding hole 231 is used for covering the notch of the mounting groove 11 when the cover plate 23 rotates, at least the reagent well 202 of the microfluidic chip 200 is exposed from the relief hole 231, when the cover plate 23 rotates to cover the notch of the mounting groove 11, the bumps tightly abut against the carrier plate 201 of the microfluidic chip 200, and press the microfluidic chip 200 against the bottom wall of the mounting groove 11, the reagent well 202 of the microfluidic chip 200 is exposed from the avoiding hole 231, and when the inlet and outlet chamber moves the microfluidic chip 200 to a designated position, the air outlet of the manifold plate faces the reagent well 202 of the microfluidic chip 200, so configured, not only the stability of the microfluidic chip 200 is ensured, but also the interference of the cover plate 23 to the reagent tank 202 of the microfluidic chip 200 is avoided.

Specifically, the cover plate 23 is rotatably installed on the base 1 through a damping rotating shaft, and when the cover plate 23 rotates to a certain angle, the damping rotating shaft enables the cover plate 23 to be automatically opened or closed under the action of self gravity, so that the user operation is more convenient.

Specifically, the installation component 100 further comprises a first magnetic attraction piece 12 and a second magnetic attraction piece 232 which are matched with each other, wherein the first magnetic attraction piece 12 and the second magnetic attraction piece 232 are arranged on the base 1, the other one is arranged on the cover plate 23, the first magnetic attraction piece 12 and the second magnetic attraction piece 232 are used for attracting each other when the cover plate 23 rotates to cover the notch of the installation groove 11, so that the cover plate 23 and the base 1 are relatively fixed, and the installation of the microfluidic chip 200 is more stable.

It should be noted that, in another embodiment, the mounting assembly 100 further includes an elastic buckle and a joint hole which are matched with each other in a clamping manner, one of the elastic buckle and the joint hole is disposed on the base 1, and the other one is correspondingly disposed on the upper cover, when the cover plate 23 rotates to cover the notch of the mounting groove 11, the elastic buckle is clamped in the joint hole, and the cover plate 23 and the base 1 are relatively fixed, so that the microfluidic chip 200 is more stably mounted.

In another embodiment, the pressing portion 21 is disposed in a block shape, the pressing portion 21 is movably mounted on the sidewall of the mounting groove 11 in the up-down direction, after the microfluidic chip 200 is placed in the mounting groove 11, the pressing portion 21 moves toward the bottom wall of the mounting groove 11, and the pressing portion 21 abuts against the carrier plate 201 of the microfluidic chip 200, so as to press the microfluidic chip 200 on the bottom wall of the mounting groove 11, thereby enabling the mounting of the microfluidic chip 200 to be more stable.

Specifically, the pressing and holding portion 21 is slidably mounted on the side wall of the mounting groove 11 in the up-down direction through a slide rail mechanism, it should be noted that the slide rail mechanism includes a slide rail and a slide groove in sliding fit, one of the slide rail and the slide groove is disposed on the pressing and holding portion 21, the other one is correspondingly disposed on the side wall of the mounting groove 11, and the movement of the pressing and holding portion 21 is smoother through the slide rail mechanism.

Referring to fig. 2 and 3, the mounting assembly 100 further includes a sealing gasket 3 movably disposed on the base 1, the sealing gasket 3 has a through hole 31 extending through a thickness direction thereof, so that when the microfluidic chip 200 is moved to a position below a manifold plate, the sealing gasket 3 is sealingly connected to the microfluidic chip 200 and the manifold plate, the through hole 31 is communicated with an air outlet of the manifold plate and a reagent tank 202 of the microfluidic chip 200, when the microfluidic chip 200 is moved to a specific position by entering and exiting from a warehouse, the air outlet of the manifold plate faces the reagent tank 202 of the microfluidic chip 200, an air pump pressurizes the reagent tank 202 of the microfluidic chip 200 through the manifold plate, during pressurization, a sample in the reagent tank 202 of the microfluidic chip 200 is easily leaked out, which not only damages the sample, but also risks contamination of other samples, thereby reducing the accuracy of preparation of a single cell library, in this embodiment, the sealing pad 3 is hermetically connected to the microfluidic chip 200 and the manifold plate, and only the through hole 31 is used to communicate the air outlet of the manifold plate with the reagent tank 202 of the microfluidic chip 200, so that the risk of leakage of the sample in the reagent tank 202 of the microfluidic chip 200 is reduced, and the risk of contamination of other samples is also reduced, thereby improving the accuracy of single-cell library preparation.

It should be noted that the material of the sealing gasket 3 is a flexible material, when the manifold plate is pressed down, the sealing gasket 3 is respectively abutted against the lower end face of the manifold plate and the upper end face of the cover plate 23, and under the extrusion of the manifold plate, the sealing gasket 3 is deformed to be connected with the microfluidic chip 200 and the manifold plate in a sealing manner.

In the present invention, the microfluidic chip 200 includes a carrier plate 201, a first side surface and a second side surface opposite to each other are formed on the carrier plate 201, a reagent tank 202 is sequentially formed on the first side surface, the reagent tank 202 includes a plurality of raw material tanks and a molding tank which are sequentially arranged, a flow channel is formed on the second side surface, the flow channel is communicated with the plurality of raw material tanks and the molding tank, different raw materials are respectively placed in the plurality of raw material tanks, and when the manifold plate 31 is pressed down, the manifold plate is combined with the microfluidic chip 200 to drive each raw material in the plurality of raw material tanks to enter the flow channel to be molded into a single cell sample, and the single cell sample flows into the molding tank.

In this embodiment, a plurality of microfluidic chips 200 are placed in the mounting groove 11 of the base 1, correspondingly, a plurality of through holes are formed in the sealing gasket 3, each of the through holes corresponds to one microfluidic chip 200, each of the through holes includes a plurality of through holes 31, and when the manifold plate 31 is pressed down, the plurality of through holes 31 respectively communicate with a plurality of outlets of the manifold plate, a plurality of corresponding raw material grooves and one molding groove, so as to hermetically connect the microfluidic chip 200 and the manifold plate.

In the present invention, the cover plate 23 includes a first side and a second side which are oppositely disposed, and a third side and a fourth side which are oppositely disposed, the first side is correspondingly rotatably mounted on one side of the base 1, the pressing portion 21 is correspondingly disposed on the second side, two mounting sets are respectively disposed on the third side and the fourth side, two matching sets are correspondingly disposed on the sealing gasket 3, and each mounting set is fixedly matched with each matching set to fixedly mount the sealing gasket 3.

Specifically, each the installation group all includes the edge first side with two installation archs 24 that the direction interval of arranging of second side set up, each the cooperation group all is including offering in two mounting holes 32 on sealed pad 3, and two mounting holes 32 correspond two the installation arch 24 sets up, is installing sealed during pad 3, will the protruding 24 cover of installation is located correspondingly in the mounting hole 32, with fixed mounting sealed pad 3.

It should be noted that, because the gasket 3 is made of a flexible material, when the gasket 3 is installed, the through holes 31 on the sealing gasket are deformed and changed in position due to the pulling force, so the specific positions and sizes of the through holes 31 need to be determined according to the materials used in practice, so as to ensure that the through hole 31 is opposite to the outlet of the corresponding multi-manifold plate and the raw material groove or the forming groove after the sealing gasket 3 is arranged on the cover plate 23, so as to hermetically connect the microfluidic chip 200 and the manifold plate, and only communicate the air outlet of the manifold plate with the reagent tank 202 of the microfluidic chip 200 through the through hole 31, thereby reducing the risk of leakage of the sample in the reagent tank 202 of the microfluidic chip 200 and the risk of contamination of other samples, and thus improving the accuracy of single-cell library preparation.

In this embodiment, the fixing structure 2 includes a cover plate 23, the pressing portion 21 includes a bump disposed on an end surface of the cover plate 23 facing the base 1, the cover plate 23 is rotatably mounted on the base 1 to be movable to open and close the notch of the mounting groove 11, and when the cover plate is movable to close the notch of the mounting groove 11, the bump abuts against the carrier plate 201 of the microfluidic chip 200; apron 23 is equipped with along its thickness direction and dodges hole 231, it is used for to dodge hole 231 apron 23 rotates to the lid and closes during the notch of mounting groove 11, supplies microfluidic chip 200's at least reagent groove 202 certainly dodge hole 231 and reveal, it is corresponding, sealed pad 3 is located apron 23 deviates from a side end face of base 1, sealed pad 3 lid is established dodge on the hole 231, sealing connection microfluidic chip 200 and manifold, through-hole 31 intercommunication the gas outlet of manifold with microfluidic chip 200's reagent groove 202 has reduced the risk that the sample in microfluidic chip 200's reagent groove 202 was let out has also reduced other samples and has received the risk of polluting to the precision of unicellular library preparation has been improved.

For further fixing microfluid chip 200, base 1 is provided with spacing portion, spacing portion be used for with locate the spacing cooperation of installation department in the business turn over storehouse, with will base 1 is fixed in business turn over storehouse, through spacing portion with the spacing cooperation of installation department makes installation component 100 is relative business turn over storehouse is fixed, makes microfluid chip 200's installation is more firm.

It should be noted that, the specific forms of the limiting portion and the mounting portion are not limited, the limiting portion may be a pin, correspondingly, the mounting portion may be a pin hole, and the pin is in limiting fit with the pin hole to fix the mounting assembly 100; spacing portion with the installation department corresponds respectively and sets up two magnetism and inhale the portion, two magnetism is inhaled the mutual actuation of portion, in order to fix installation component 100, in this embodiment, spacing portion sets up to the elasticity card post, and is corresponding, the installation department is for offering in the card hole on the bottom plate in business turn over storehouse, the elasticity card post with card hole is held each other the card, in order to fix installation component 100 makes microfluid chip 200's installation is more firm.

The above description is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention, and all modifications and equivalents of the present invention, which are made by the contents of the present specification and the accompanying drawings, or directly/indirectly applied to other related technical fields, are included in the scope of the present invention.

Claims

1. A mounting assembly for mounting a microfluidic chip, comprising:

2. The mounting assembly of claim 1, wherein the securing structure further comprises a positioning portion disposed within the mounting slot for positioning engagement with an engagement portion disposed on the microfluidic chip.

3. The mounting assembly of claim 1, wherein the fixing structure comprises a cover plate, the pressing portion comprises a protrusion disposed on an end surface of the cover plate facing the base, the cover plate is rotatably mounted on the base to be movable to open and close the notch of the mounting groove, and the protrusion abuts against the carrier plate of the microfluidic chip when the cover plate is movable to close the notch of the mounting groove;

4. The mounting assembly of claim 3 wherein the cover plate is rotatably mounted to the base by a damped rotation shaft.

5. The mounting assembly of claim 3, further comprising a first magnetic element and a second magnetic element that are engaged with each other, wherein one of the first magnetic element and the second magnetic element is disposed on the base, and the other one of the first magnetic element and the second magnetic element is disposed on the cover plate, and the first magnetic element and the second magnetic element are engaged with each other when the cover plate rotates to cover the opening of the mounting groove.

6. The mounting assembly of claim 1, wherein the pressing portion is provided in a block shape, and the pressing portion is movably mounted on a side wall of the mounting groove in an up-down direction.

7. The mounting assembly of claim 6, wherein the pressing portion is slidably mounted to the side wall of the mounting groove in an up-down direction by a slide rail mechanism.

8. The mounting assembly of claim 1, further comprising a sealing pad movably disposed on the base, wherein the sealing pad has a through hole extending therethrough along a thickness direction thereof, so that when the microfluidic chip is moved under the manifold plate, the sealing pad sealingly connects the microfluidic chip and the manifold plate, and the through hole communicates an air outlet of the manifold plate with a reagent reservoir of the microfluidic chip.

9. The mounting assembly of claim 8, wherein the fixing structure comprises a cover plate rotatably mounted on the base, the cover plate having an avoiding hole formed therethrough along a thickness direction thereof;

10. The mounting assembly of claim 1 wherein the base is provided with a limiting portion for limiting engagement with a mounting portion provided in an in-out housing to secure the base to the in-out housing.