CN211463198U - Sealing gasket and microfluidic chip assembly with same - Google Patents
Sealing gasket and microfluidic chip assembly with same Download PDFInfo
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- CN211463198U CN211463198U CN201922341262.5U CN201922341262U CN211463198U CN 211463198 U CN211463198 U CN 211463198U CN 201922341262 U CN201922341262 U CN 201922341262U CN 211463198 U CN211463198 U CN 211463198U
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Abstract
The utility model relates to a sealed pad and have its micro-fluidic chip subassembly. The sealing gasket is used for being matched with a micro-fluidic chip, the micro-fluidic chip comprises a chip substrate and a liquid storage pool group arranged on the chip substrate, the liquid storage pool group comprises an oil phase liquid storage pool, a water phase liquid storage pool and a micro-droplet liquid storage pool, one surface of the sealing gasket is provided with a sealing hole group corresponding to the liquid storage pool group, the sealing hole group comprises a first sealing hole, a second sealing hole and a third sealing hole, the first sealing hole, the second sealing hole and the third sealing hole respectively correspond to the open ends of the oil phase liquid storage pool, the water phase liquid storage pool and the micro-droplet liquid storage pool, the other surface of the sealing gasket is also provided with through holes respectively corresponding to and communicated with the first sealing hole, the second sealing hole and the third sealing hole, and the through holes are used for being in butt. The utility model discloses a sealed pad can realize the high-speed joint between micro-fluidic chip and the droplet generation device to still be difficult to make mistakes.
Description
Technical Field
The utility model relates to a micro-fluidic chip technical field especially relates to a sealed pad and have its micro-fluidic chip subassembly.
Background
The micro-fluidic chip technology integrates basic operation units such as sample preparation, reaction, separation, detection and the like in analysis processes such as biology, chemistry, medicine and the like on a micron-scale chip to automatically complete the whole analysis process. The microfluidic chip generally includes a chip substrate and a plurality of reservoirs disposed on the chip substrate. In the using process, a user is usually required to manually connect the conduits of the droplet generation device with the respective liquid storage tanks on the microfluidic chip in sequence, especially for a multi-channel microfluidic chip, the number of the conduits to be connected is large, which is time-consuming in operation, and due to the large number, errors are easy to occur, which leads to errors in final droplet generation.
SUMMERY OF THE UTILITY MODEL
In view of the above, there is a need to provide a sealing pad and a microfluidic chip assembly having the same, which can achieve fast connection between the microfluidic chip and the droplet generation device and is also not prone to error.
A sealing pad is used for being matched with a micro-fluidic chip, the micro-fluidic chip comprises a chip substrate and a liquid storage pool group arranged on the chip substrate, the liquid storage pool group comprises an oil phase liquid storage pool, a water phase liquid storage pool and a micro-droplet liquid storage pool, one side of the sealing pad is provided with a sealing hole group corresponding to the liquid storage pool group, the sealing hole group comprises a first sealing hole, a second sealing hole and a third sealing hole, the first sealing hole, the second sealing hole and the third sealing hole respectively correspond to and seal the opening ends of the oil phase liquid storage pool, the water phase liquid storage pool and the micro-droplet liquid storage pool, the other side of the sealing pad is further provided with through holes respectively corresponding to and communicated with the first sealing hole, the second sealing hole and the third sealing hole, and the through holes are used for being in butt joint with a guide pipe of a micro-droplet generating device.
The sealing pad can directly cover the top of the microfluidic chip when in use, the oil phase liquid storage pool, the water phase liquid storage pool and the micro-droplet liquid storage pool can be simultaneously sealed through the sealing hole group on the sealing pad and the liquid storage pool group on the chip substrate in sealing fit, and then the guide pipe of the micro-droplet generating device is butted with the through hole on the sealing pad, so that the microfluidic chip and the micro-droplet generating device are quickly connected, the operation is simple and convenient, and a group of sealing hole groups corresponds to a group of liquid storage pool groups, so that errors are not easy to occur.
In one embodiment, the sealing gasket comprises a base plate, and a first sleeve, a second sleeve and a third sleeve which are arranged on the same surface of the base plate, wherein one ends of the first sleeve, the second sleeve and the third sleeve, which are far away from the base plate, are open, and inner cavities of the first sleeve, the second sleeve and the third sleeve respectively form the first sealing hole, the second sealing hole and the third sealing hole.
In one embodiment, the first sealing hole, the second sealing hole and the third sealing hole are counter bores recessed inwards from one surface of the sealing gasket.
In one embodiment, one side of the gasket is provided with a lug.
In one embodiment, the sealing gasket is a rubber sealing gasket or a silica gel sealing gasket.
In one embodiment, the sealing pad is composed of a single group of the sealing holes, and the sealing pad is used for being matched with the single group of the liquid storage pool on the chip substrate in a sealing mode.
In one embodiment, the sealing pad comprises at least two sets of the sealing holes, and the sealing pad is used for simultaneously and hermetically matching with at least two sets of the liquid storage batteries on the chip substrate.
In one embodiment, the number of the sealing hole groups on the sealing gasket corresponds to the number of the liquid storage battery groups on the chip substrate one by one, and the sealing gasket is used for being matched with all the liquid storage battery groups on the chip substrate in a sealing mode.
A microfluidic chip assembly comprises a microfluidic chip and the sealing gasket matched with the microfluidic chip.
In one embodiment, the microfluidic chip is made of a hard plastic material or a glass material.
Drawings
Fig. 1 is a schematic structural diagram of a microfluidic chip according to an embodiment;
fig. 2 is a schematic structural view of a gasket according to a first embodiment of the present invention;
FIG. 3 is a schematic view of the gasket of FIG. 2 from another perspective;
fig. 4 is a schematic structural view of a gasket according to a second embodiment of the present invention;
FIG. 5 is a schematic view of the gasket of FIG. 4 from another perspective;
fig. 6 is a schematic diagram of a microfluidic chip assembly according to an embodiment of the present invention;
FIG. 7 is a schematic diagram of the microfluidic chip assembly of the first embodiment in cooperation with a droplet generation apparatus;
FIG. 8 is a schematic diagram of a second embodiment of a microfluidic chip assembly in cooperation with a droplet generation device;
fig. 9 is a schematic diagram of a microfluidic chip assembly and a droplet generation device according to a third embodiment.
10. The micro-fluidic chip comprises a micro-fluidic chip, 11, a chip substrate, 12, a liquid storage pool group, 121, an oil phase liquid storage pool, 122, a water phase liquid storage pool, 123, a droplet liquid storage pool, 20, a sealing pad, 201, a first sealing hole, 202, a second sealing hole, 203, a third sealing hole, 204, a through hole, 21, a backing plate, 22, a first sleeve, 23, a second sleeve, 24, a third sleeve, 25, a lug, 30 and a droplet generation device.
Detailed Description
In order to facilitate understanding of the present invention, the present invention will be described more fully hereinafter with reference to the accompanying drawings. The preferred embodiments of the present invention are shown in the drawings. The invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. In contrast, when an element is referred to as being "directly on" another element, there are no intervening elements present. In the present invention, the terms "first", "second" and "third" do not denote any particular quantity or order, but are merely used to distinguish names.
Referring to fig. 1 and 6, a gasket 20 is used for cooperating with a microfluidic chip 10, the microfluidic chip 10 includes a chip substrate 11 and a liquid storage pool 12 disposed on the chip substrate 11, and the liquid storage pool 12 includes an oil phase liquid storage pool 121, a water phase liquid storage pool 122, and a droplet liquid storage pool 123. The chip substrate 11 is provided with a microfluidic channel for liquid to flow through, each liquid storage tank of the liquid storage tank group 12 is in a cylindrical shape with an open top, and the bottom of each liquid storage tank is communicated with the microfluidic channel in the chip substrate 11. The microfluidic chip 10 may be made of a hard plastic material, such as resin materials, e.g., PDMS (polydimethylsiloxane), PMMA (polymethyl methacrylate), PC (polycarbonate), COC (cyclic olefin copolymer), etc.; or the microfluidic chip 10 can be made of glass.
The microfluidic chip 10 may be a single-channel microfluidic chip 10 or a multi-channel microfluidic chip 10. For a single-channel microfluidic chip 10, a chip substrate 11 is provided with a group of liquid storage pool groups 12, and one micro-droplet can be generated each time; for the multi-channel micro-fluidic chip 10, two or more liquid storage pool groups 12 are arranged on the chip substrate 11, and two or more micro-droplets can be generated each time. The following description will mainly take the multi-channel microfluidic chip 10 as an example.
Specifically, as shown in fig. 1, the microfluidic chip 10 includes a chip substrate 11 and a plurality of liquid storage groups 12 disposed on the chip substrate 11, the liquid storage groups 12 are sequentially arranged along a length direction of the chip substrate 11, and each liquid storage group 12 includes an oil phase liquid storage tank 121, a water phase liquid storage tank 122, and a droplet liquid storage tank 123 that are sequentially arranged along a width direction of the chip substrate 11. The specific number of the liquid storage pools 12 can be set according to actual requirements, for example, 8 liquid storage pools 12 are provided on the chip substrate 11 in fig. 1, and 8 droplets can be generated each time.
One surface of the gasket 20 is provided with a sealing hole group corresponding to the liquid storage cell group 12. Specifically, a single group of sealing holes (as shown in fig. 3) may be disposed on one side of the sealing pad 20, and may correspond to and be in sealing fit with one group of liquid storage groups 12 of the microfluidic chip 10, so that a plurality of liquid storage groups 12 of the microfluidic chip 10 may be sealed only by using a plurality of sealing pads 20 at the same time; alternatively, one surface of the sealing pad 20 is provided with a plurality of sealing hole sets (as shown in fig. 5), and the plurality of sealing hole sets correspond to the plurality of liquid storage cell sets 12 of the microfluidic chip 10 one by one, so that the plurality of liquid storage cell sets 12 of the microfluidic chip 10 can be simultaneously sealed by using only one sealing pad 20. Optionally, the whole gasket 20 is made of a flexible material, so as to perform the sealing and buffering functions. Of course, the flexible layer may be provided only on the inner wall surface of the sealing hole to perform the sealing function.
Referring to fig. 2 to 5, the sealing hole set includes a first sealing hole 201, a second sealing hole 202, and a third sealing hole 203, where the first sealing hole 201, the second sealing hole 202, and the third sealing hole 203 correspond to the open ends of the oil phase reservoir 121, the water phase reservoir 122, and the droplet reservoir 123, respectively. The other side of the sealing pad 20 is further provided with a through hole 204 corresponding to and communicating with the first sealing hole 201, the second sealing hole 202 and the third sealing hole 203, respectively, and the through hole 204 is used for butting with the conduit of the droplet generating device 30. The specific sizes of the first sealing hole 201, the second sealing hole 202 and the third sealing hole 203 are matched with the sizes of the corresponding liquid storage tanks, so that sealing can be realized. When the liquid storage tank is used, the open ends of the liquid storage tanks are sealed and inserted into the corresponding sealing holes, so that good sealing performance of the liquid storage tanks can be guaranteed in the droplet generation process. In the process of droplet generation, inert gas is respectively introduced into the oil phase reservoir 121 and the water phase reservoir 122 through the conduit of the droplet generation device 30, so that the oil phase and the water phase are converged, droplets are generated by shearing force, and finally the droplets are converged into the droplet reservoir 123.
When in use, the sealing pad 20 can directly cover the top of the microfluidic chip 10, the oil phase liquid storage tank 121, the water phase liquid storage tank 122 and the droplet liquid storage tank 123 can be simultaneously sealed by the sealing hole group on the sealing pad 20 in sealing fit with the liquid storage tank group 12 on the chip substrate 11, and then the conduit of the droplet generation device 30 is butted with the through hole 204 on the sealing pad 20, so that the microfluidic chip 10 and the droplet generation device 30 are quickly connected, the operation is simple and convenient, and a group of sealing hole groups corresponds to a group of liquid storage tank groups 12, so that errors are not easy to occur.
Referring to fig. 2 and 3, in the first embodiment, the sealing gasket 20 includes a backing plate 21, and a first sleeve 22, a second sleeve 23, and a third sleeve 24 disposed on the same surface of the backing plate 21, wherein ends of the first sleeve 22, the second sleeve 23, and the third sleeve 24, which are far away from the backing plate 21, are open, and inner cavities of the first sleeve 22, the second sleeve 23, and the third sleeve 24 respectively form a first sealing hole 201, a second sealing hole 202, and a third sealing hole 203. When the micro-fluidic chip is used, the sealing gasket 20 is aligned with the liquid storage pool group 12 on the chip substrate 11 and then pressed downwards, so that the first sleeve 22 is sleeved at the open end of the oil phase liquid storage pool 121, the second sleeve 23 is sleeved at the open end of the water phase liquid storage pool 122, and the third sleeve is sleeved at the open end of the micro-droplet liquid storage pool 123, thereby realizing the sealing connection between the sealing gasket 20 and the micro-fluidic chip 10, and being simple and convenient to operate.
For another example, in the second embodiment, as shown in fig. 5, each of the first seal hole 201, the second seal hole 202, and the third seal hole 203 is a counter bore recessed inward from one surface of the gasket 20.
Further, as shown in fig. 5, one side of the packing 20 is provided with a lug 25. When the sealing pad 20 needs to be removed, the operator can easily separate the sealing pad 20 from the microfluidic chip 10 by simply pinching the lug 25 and lifting it upward.
Alternatively, the gasket 20 is a rubber gasket or a silicone gasket. During the droplet generating process, the sealing and buffering functions can be simultaneously realized.
Referring to fig. 2 and 3, in one embodiment, the sealing pad 20 is formed by a single set of sealing holes, and the sealing pad 20 is used to be matched with the single set of liquid storage cells 12 on the chip substrate 11 in a sealing manner. When in use, the corresponding number of sealing gaskets 20 can be configured according to the number of the droplets to be generated, and the flexibility is strong. For example, as shown in fig. 7, when only one droplet needs to be generated, only one sealing pad 20 is used to seal a group of reservoir groups 12 on the microfluidic chip 10, and then the conduit of the droplet generation device 30 is abutted to the corresponding through hole 204 on the sealing pad 20, and inert gas is respectively introduced into the oil phase reservoir 121 and the water phase reservoir 122 through the conduit of the droplet generation device 30, so that the oil phase and the water phase are converged, droplets are generated by shearing force, and finally converged into the droplet reservoir 123. For another example, as shown in fig. 8, when it is required to generate 8 droplets, 8 sealing pads 20 may be respectively matched with 8 sets of reservoir sets 12 on the microfluidic chip 10 in a sealing manner.
Referring to fig. 6, in another embodiment, the sealing gasket 20 includes at least two sets of sealing holes, and the sealing gasket 20 is used for simultaneously sealing and matching with at least two sets of liquid storage sets 12 on the chip substrate 11. In this manner, a plurality of gaskets 20 having different numbers of seal hole groups may be used in combination to seal different numbers of reservoir groups 12.
Further, referring to fig. 5 and fig. 9, the number of the sealing hole groups on the sealing gasket 20 corresponds to the number of the liquid storage cell groups 12 on the chip substrate 11, and the sealing gasket 20 is used for simultaneously sealing and matching with all the liquid storage cell groups 12 on the chip substrate 11. Therefore, all the liquid storage tank groups 12 can be sealed only by one sealing pad 20, and then the conduits of the droplet generation device 30 are butted with the corresponding through holes 204 on the sealing pad 20, inert gas is respectively introduced into the oil phase liquid storage tank 121 and the water phase liquid storage tank 122 through the conduits of the droplet generation device 30, so that the oil phase and the water phase are converged, droplets are generated through shearing force and finally converged into the droplet liquid storage tank 123, the operation is simple and convenient, time and labor are saved, and the method can be well applied to the multi-channel microfluidic chip 10.
Referring to fig. 6, the present invention further provides a microfluidic chip assembly. The microfluidic chip assembly comprises a microfluidic chip 10 and a sealing gasket 20 matched with the microfluidic chip 10. The specific structure of the microfluidic chip 10 and the sealing pad 20 can refer to the above embodiments. Since the microfluidic chip assembly adopts all technical schemes of the above embodiments, at least all beneficial effects brought by the above technical schemes are achieved, and no further description is given here.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only represent some embodiments of the present invention, and the description thereof is specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.
Claims (10)
1. A sealing gasket is used for being matched with a micro-fluidic chip, the micro-fluidic chip comprises a chip substrate and a liquid storage pool group arranged on the chip substrate, the liquid storage pool group comprises an oil phase liquid storage pool, a water phase liquid storage pool and a micro-droplet liquid storage pool, and the sealing gasket is characterized in that one surface of the sealing gasket is provided with a sealing hole group corresponding to the liquid storage pool group, the sealing hole group comprises a first sealing hole, a second sealing hole and a third sealing hole, the first sealing hole, the second sealing hole and the third sealing hole respectively correspond to and seal open ends of the oil phase liquid storage pool, the water phase liquid storage pool and the micro-droplet liquid storage pool, the other surface of the sealing gasket is further provided with through holes respectively corresponding to and communicated with the first sealing hole, the second sealing hole and the third sealing hole, and the through holes are used for being in butt joint with a guide pipe of a micro-droplet generating device.
2. The gasket of claim 1, wherein the gasket comprises a gasket plate, and a first sleeve, a second sleeve and a third sleeve which are disposed on the same surface of the gasket plate, wherein the ends of the first sleeve, the second sleeve and the third sleeve, which are far away from the gasket plate, are open, and inner cavities of the first sleeve, the second sleeve and the third sleeve respectively form the first sealing hole, the second sealing hole and the third sealing hole.
3. The gasket of claim 1 wherein said first sealing aperture, said second sealing aperture and said third sealing aperture are counterbores recessed inwardly from a face of said gasket.
4. A gasket according to claim 1, characterized in that one side of the gasket is provided with a lug.
5. A gasket according to claim 1, wherein the gasket is a rubber gasket or a silicone gasket.
6. A seal according to any one of claims 1 to 5, wherein said seal is formed from a single set of said sealing apertures, said seal being adapted to sealingly engage a single set of said reservoirs on said chip substrate.
7. A seal according to any one of claims 1 to 5, wherein said seal comprises at least two sets of said sealing holes, said seal being adapted to simultaneously sealingly engage at least two sets of said reservoirs on said chip substrate.
8. The gasket of claim 7 wherein the number of said sealing aperture sets corresponds one-to-one to the number of said reservoir sets on said chip substrate, said gasket being adapted to simultaneously sealingly engage all of said reservoir sets on said chip substrate.
9. A microfluidic chip assembly comprising a microfluidic chip and a gasket according to any one of claims 1 to 8 mated to the microfluidic chip.
10. The microfluidic chip assembly according to claim 9, wherein the microfluidic chip is made of a rigid plastic material or a glass material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922341262.5U CN211463198U (en) | 2019-12-24 | 2019-12-24 | Sealing gasket and microfluidic chip assembly with same |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922341262.5U CN211463198U (en) | 2019-12-24 | 2019-12-24 | Sealing gasket and microfluidic chip assembly with same |
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| CN211463198U true CN211463198U (en) | 2020-09-11 |
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| CN201922341262.5U Active CN211463198U (en) | 2019-12-24 | 2019-12-24 | Sealing gasket and microfluidic chip assembly with same |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022151537A1 (en) * | 2021-01-13 | 2022-07-21 | 于海 | Digital pcr micro-droplet generating device |
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2019
- 2019-12-24 CN CN201922341262.5U patent/CN211463198U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022151537A1 (en) * | 2021-01-13 | 2022-07-21 | 于海 | Digital pcr micro-droplet generating device |
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