CN215866747U - Novel liquid drop sampling gun head and sampling system comprising same - Google Patents
Novel liquid drop sampling gun head and sampling system comprising same Download PDFInfo
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- CN215866747U CN215866747U CN202122001343.8U CN202122001343U CN215866747U CN 215866747 U CN215866747 U CN 215866747U CN 202122001343 U CN202122001343 U CN 202122001343U CN 215866747 U CN215866747 U CN 215866747U
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Abstract
The utility model provides a novel liquid drop sampling gun head and a sampling system comprising the same, wherein the liquid drop sampling gun head comprises: an axially extending outer pipe; the inner layer capillary tube is fixed in the outer layer pipeline and extends coaxially with the outer layer pipeline; a support for fixing the inner layer capillary tube relative to the outer layer pipeline; the hydrophilic filtering structure fixed at the front end of the outer layer pipeline keeps a certain distance from the front end of the inner layer capillary; the oil phase is filled between the outer pipeline and the inner capillary, the outer pipeline is connected with the oil storage device, the inner capillary is connected with the negative pressure device, and when the front end of the sampling gun head is immersed in a water phase sample to be collected, the water phase sample can be collected into the inner capillary in a liquid drop mode and is continuously conveyed from the front end to the rear end. The utility model provides the sampling gun head which has the advantages of reduced manufacturing cost and capability of directly sampling liquid drops, and can more efficiently collect and store water phase samples in a liquid drop mode without pollution and assist in improving the detection sensitivity.
Description
Technical Field
The utility model relates to the field of liquid sampling devices, in particular to a novel liquid drop sampling gun head and a sampling system comprising the same.
Background
The existing sampling device mainly comprises a hollow pipette tip or a hollow needle head, wherein negative pressure is used as power to suck a liquid sample to be temporarily stored in a hollow channel for waiting transfer. It has three problems: firstly, because only one reaction can be carried out on one sample, the detection flux is low, a sufficient amount of sample can obtain a reliable detection result, the sensitivity is low, and the required sample volume is large; secondly, the problem of mutual pollution is difficult to avoid in the process of collecting multiple samples, so that high-flux simultaneous sampling and detection of multiple samples cannot be realized; thirdly, the sample directly contacts with the inner wall of the pipeline, and the sample loss problem exists in the transferring and transporting process.
To date, there have been some proposals for high-throughput, small sample, high-sensitivity detection, such as digital PCR, by a method of generating droplets by a droplet generation chip. However, in these solutions, the collected sample needs to be introduced into an additional droplet generation chip to obtain droplets, and the sample and the reagent need to be manually mixed to generate droplets, which is costly, complicated in steps, and unable to avoid the problems of sample loss and contamination during the transfer process. These drawbacks not only limit the accuracy of the final detection, but also limit its application.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a novel liquid drop sampling gun head and a sampling system comprising the same, so that the problems of low detection flux, low sensitivity, sample pollution, sample loss, high cost and complicated steps in the prior art are solved.
In order to solve the technical problems, the utility model adopts the following technical scheme:
according to a first aspect of the present invention, there is provided a novel liquid droplet sampling gun head comprising: an axially extending outer pipe; the inner layer capillary tube is fixed in the outer layer pipeline and extends coaxially with the outer layer pipeline; a support for securing the inner capillary relative to the outer tube; the hydrophilic filtering structure is fixed at the front end of the outer layer pipeline, and a certain distance is kept between the hydrophilic filtering structure and the front end of the inner layer capillary; the sampling gun head comprises an outer layer pipeline, an inner layer capillary tube, a negative pressure device, an oil storage device, a sampling gun head, a liquid phase sample collecting device and a control device, wherein an oil phase is filled between the outer layer pipeline and the inner layer capillary tube, the far end of the outer layer pipeline is connected with the oil storage device, the far end of the inner layer capillary tube is connected with the negative pressure device, and when the front end of the sampling gun head is immersed in the water phase sample to be collected, the water phase sample can be collected into the inner layer capillary tube in a liquid drop mode at intervals through the oil phase and is continuously conveyed from the front end to the rear end.
Preferably, the holder is a sheet having a through hole, at least a portion of the outer periphery of the sheet being connected to the outer tube, and the inner capillary tube passes through the through hole and is fixedly connected to the holder at the through hole.
Preferably, the structure of the sampling gun head except the hydrophilic filtering structure can be integrally formed through 3D printing. It will be appreciated that the novel drop sampling tip of the present invention is not limited to being integrally formed by 3D printing.
Preferably, the outer pipe has a structure with a thin front end and a thick rear end.
Preferably, the hydrophilic filtering structure is fixed with the front end of the outer-layer pipeline through ultraviolet light curing glue.
Preferably, the hydrophilic filtering structure and the front end of the inner layer capillary tube are kept between 1 and 3 mm.
According to a second aspect of the present invention, there is provided a sampling system comprising the sampling lance head, comprising: the sampling gun head, the analysis device, the collecting bottle and the negative pressure equipment are sequentially connected through a pipeline, and the oil storage bottle is connected with an outer layer pipeline of the sampling gun head; wherein, the analysis device is connected with the inner layer capillary of the sampling gun head.
Preferably, the method further comprises the following steps: an oil phase input pipeline led in by a joint beside a sample inlet pipeline between the sampling gun head and the input end of the analysis device, and a reagent input pipeline led in by the oil phase input pipeline by passing through another joint, so that a detection reagent droplet wrapped by the oil phase enters the sample inlet pipeline and is mixed with the sample droplet on line.
The negative pressure device is selected from: any one of a negative pressure pump, a syringe and an automatic pipetting workstation.
According to a third aspect of the present invention, there is provided a high-throughput sampling method, comprising the steps of: s1: providing the sampling gun head; s2: connecting an outer layer pipeline of the sampling gun head with oil storage equipment, filling an oil phase between the outer layer pipeline and an inner layer capillary tube, and connecting the inner layer capillary tube with negative pressure equipment; s3: and the front end of the sampling gun head is immersed into a water phase sample to be collected, so that the water phase sample can be collected into the inner capillary in a liquid drop form wrapped by the oil phase, and the high-flux sampling of the water phase sample is realized.
Preferably, the method further comprises step S4: and fusing the sample liquid drop collected in the step S3 with the detection reagent liquid drop introduced by another sample adding pipeline, so that the online mixing of the sample and the detection reagent can be realized.
According to the novel liquid drop sampling gun head provided by the utility model, the working principle is that a water phase sample is cut and wrapped by an oil phase after passing through a hydrophilic filtering structure arranged at the front end of the sampling gun head, and is transported to the rear end in a liquid drop mode. The sampling gun head can prevent the problems of pollution and leakage among multiple samples, and sample liquid drops can be directly mixed with other detection reagents for reaction and then detected at the rear end, so that the operation steps for generating the liquid drops are simplified. A large number of liquid drops are used as a reaction and detection unit, so that the subsequent detection sensitivity can be improved, wherein the hydrophilic filtering structure can ensure that only the water phase passes through but the oil phase cannot pass through.
It is expected that if the sampling gun head provided by the utility model is adopted to replace the traditional needle head at the front end of the automatic pipetting system, the automatic, high-flux and digital quantitative sampling can be realized, or the sampling gun head is combined with a handheld pipetting gun, and the on-site rapid sampling can be realized.
Compared with the existing water phase sample sampling system, the sampling system has the following advantages:
1) liquid drops are directly generated after sampling, so that the steps of generating a chip by the liquid drops and transferring a sample are omitted, and the sampling process is simplified;
2) the water phase liquid sample is wrapped and carried by the immiscible oil phase, so that the sample cannot contact with the outside in the transferring and transporting processes, the problems of pollution and leakage can be avoided, and meanwhile, the sample cannot remain on the inner wall of the pipeline, so that the sample loss is reduced, even a nanoliter-level sample can be completely transported to the rear end after being collected, and the sampling method has great significance for sampling a trace sample;
3) one sample is divided into thousands of samples by liquid drops, essentially, one reaction is divided into a plurality of independent and simultaneous reactions, target molecules are respectively detected in each reaction unit, and signals of each reaction unit can be statistically analyzed after detection, so that the method lays a foundation for realizing high-flux and digital quantitative detection, improves flux and sensitivity, and reduces the requirement on the volume of the required sample;
4) after sampling, the liquid drops can be mixed and reacted with the detection reagent on line, so that the step of manually adding the reaction reagent to each reaction unit for mixing after sampling is omitted, and the system integration level is improved;
5) the special hydrophilic filtering structure of the gun head can prevent oil phase from leaking out of the sampling gun and avoid pollution to the sampling environment;
6) simple structure, low cost, easy batch manufacture and wide application.
In summary, the utility model provides a sampling gun head and a sampling system which have reduced manufacturing cost and can directly sample liquid drops, can more efficiently, pollution-free and sensitively collect, store and detect a water phase sample in a liquid drop form, has the advantages of simple, rapid, efficient and pollution-free sampling and detecting processes, and can replace the existing sampling device.
Drawings
Fig. 1 is a schematic structural view of a novel liquid droplet sampling gun head according to a preferred embodiment of the present invention;
FIG. 2 is a schematic view of the working principle of droplet sampling using the liquid sampling gun head shown in FIG. 1, wherein a-oil phase filling, b-sample collection, c-droplet generation, d-droplet transportation;
FIG. 3 is a schematic diagram of the working principle of droplet output using the sampling gun head shown in FIG. 1, wherein e-oil phase filling, f-droplet input, and h-droplet output is completed during g-droplet output;
FIG. 4 is a schematic diagram of a sampling system in accordance with a preferred embodiment of the present invention;
FIG. 5 is a schematic diagram of the working principle of the online mixing reaction of the sample droplet and the detection reagent droplet.
Detailed Description
The present invention will be further described with reference to the following specific examples. It should be understood that the following examples are illustrative only and are not intended to limit the scope of the present invention.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like, indicate orientations and positional relationships based on those shown in the drawings, and are used only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be considered as limiting the present invention.
Referring to fig. 1, a novel liquid drop sampling gun head according to a preferred embodiment of the present invention comprises: the device comprises an outer-layer pipeline 1, an inner-layer capillary tube 2, a bracket 3 and a hydrophilic filtering structure 4.
Wherein, outer pipeline 1 has the thin, the thick tubular structure of rear end in front end, and inlayer capillary 2 is fixed in outer pipeline 1 to with outer pipeline 1 coaxial extension, the rear end of inlayer capillary 2 is fixed through support 3 relative outer pipeline 1, hydrophilic filtration 4 is fixed in the front end of outer pipeline 1, and keeps the certain distance with the front end of inlayer capillary 2.
According to the preferred embodiment, as shown in fig. 1, the holder 3 is a rectangular sheet having a circular through hole 31, the outer periphery of the short side of the rectangular sheet is connected to the inner wall of the outer tube 1, and the inner capillary 2 passes through the through hole 31 and is fixedly connected to the holder 3 at the through hole 31. Optionally, the aforementioned connection manner is glue connection. It should be understood that the support 3 is not limited to this shape, but is only exemplary and not limiting.
According to this preferred embodiment, the material is preferably a transparent plastic. It is also preferred that the entire sampling tip except the hydrophilic filter structure 4 is also integrally formed by 3D printing.
It should be understood, however, that the sampling tip is not limited to being integrally formed by 3D printing.
According to the preferred embodiment, the outer tube 1 is a tubular structure with a thin front end and a thick rear end, and the area of the tip of the gun head is reduced to facilitate sampling of precise positions. The outer pipe 1 has a minimum diameter of about 2mm, a maximum diameter of about 8mm and an overall length of about 5 cm. It is to be understood that this is by way of illustration and not of limitation.
The hydrophilic filtering structure 4 may adopt a commercial filtering membrane, or a hydrophilic structure made of porous silicon, a chemical synthetic polymer material, or the like, which allows only passage of the aqueous phase, but does not allow passage of the oil phase.
According to the preferred embodiment, the commercial filtering membrane is cut into a circular structure slightly larger than the outer diameter of the front end of the outer-layer pipeline 1, then the outer-layer pipeline 1 is pressed on the filtering membrane, a circle of glue in contact with the filtering membrane is dispensed by ultraviolet curing glue, and the ultraviolet curing glue is irradiated under an ultraviolet lamp for about ten seconds to obtain the novel liquid drop sampling gun head covering the hydrophilic filtering structure 4. It should be understood that, by way of example only and not limitation, the hydrophilic filter structure 4 and the front end of the outer pipe 1 are not limited to being fixed by ultraviolet light curing glue, and other connection methods can be adopted.
According to the novel liquid drop sampling gun head provided by the preferred embodiment, the working principle is as shown in a, b, c and d in fig. 2, when the novel liquid drop sampling gun head is used, an oil phase is filled between the outer layer pipeline 1 and the inner layer capillary tube 2, the outer layer pipeline 1 is connected with an oil storage device, the inner layer capillary tube 2 is connected with a negative pressure device, when the front end of the sampling gun head is immersed in a water phase sample to be collected for sampling, a continuous water phase entering the sampling device is sucked into the inner layer capillary tube 2, and is cut into water phase liquid drops which are separated from each other by the oil phase entering from the outer layer pipeline 1, and the water phase liquid drops wrapped by the oil phase are further continuously conveyed from the front end to the rear end of the sampling gun head, so that the sampling in a liquid drop form is realized.
Conversely, when the sampling gun head is used for outputting liquid drops outwards, the working principle is shown as e, f, g and h in fig. 3, in the process, the capillary tube 2 in the inner layer is in positive pressure, the water phase liquid drops wrapped by the oil phase are continuously conveyed from the rear end to the front end of the sampling gun head, only the water phase liquid drops are output from the capillary tube 2 in the inner layer, and the oil phase is recovered by the outer layer pipeline 1.
As shown in fig. 4, a sampling system according to a preferred embodiment of the present invention includes: the sampling gun head 10, the analysis device 20, the collecting bottle 30, the negative pressure equipment 40 and the oil storage bottle 50 are sequentially connected through pipelines, wherein the oil storage bottle 50 is connected with the outer layer pipeline 1 of the sampling gun head 10; wherein, the analysis device 20 is connected with the inner layer capillary 2 of the sampling gun head 10.
The use method of the sampling system is as follows: firstly, the components are connected as shown in fig. 4, under the action of an oil storage bottle 50, oil phase is filled in a space between an outer layer pipeline 1 and an inner layer capillary 2 of a sampling gun head 10, then the sampling gun head 10 is immersed in a water phase sample to be collected, then a negative pressure device 40 is started, continuous water phase entering the sampling gun head 10 is sucked into the inner layer capillary 2 and is cut into interval water phase liquid drops by the oil phase entering from the outer layer pipeline 1, the water phase liquid drops are further continuously conveyed from the front end to the rear end of the sampling gun head 10 and enter an analysis device 20 through a pipeline, detection of the liquid drop sample can be realized, the liquid drops after detection flow out from the output end of the analysis device 20, and then are recovered through a collection bottle 30.
According to a preferred embodiment of the present invention, the sampling system further comprises: a sample addition pipeline 60 introduced beside the sample introduction pipeline between the sampling gun head 10 and the input end of the analysis device 20, wherein the sample addition pipeline 60 comprises: an oil phase input line 61 in communication with the sample injection line, and a reagent input line 62 in communication with the oil phase input line 61.
According to the sampling system, the working principle is shown in fig. 5, a water phase sample is sheared into water phase sample liquid drops D2 which are spaced from each other by an oil phase entering from an outer layer pipeline 1, a detection reagent forms a detection reagent liquid drop D1 under the shearing action of the oil phase, the detection reagent liquid drop D1 is mixed with the water phase sample liquid drop D2 to form a large liquid drop D3, the online mixing reaction of the sampled sample liquid drop and the detection reagent is realized, the step of manually adding a reaction reagent to each reaction unit for mixing after sampling is omitted, and the system integration level is improved.
In the technical scheme, the size of a liquid drop formed by the sample and the detection reagent can be controlled by pressure, so that the mixing reaction with controllable volume is realized.
The above embodiments are merely preferred embodiments of the present invention, which are not intended to limit the scope of the present invention, and various changes may be made in the above embodiments of the present invention. All simple and equivalent changes and modifications made according to the claims and the content of the specification of the present application fall within the scope of the claims of the present patent application. The utility model has not been described in detail in order to avoid obscuring the utility model.
Claims (8)
1. A novel liquid drop sampling rifle head which characterized in that includes:
an axially extending outer pipe;
the inner layer capillary tube is fixed in the outer layer pipeline and extends coaxially with the outer layer pipeline;
a support for securing the inner capillary relative to the outer tube;
the hydrophilic filtering structure is fixed at the front end of the outer layer pipeline, and a certain distance is kept between the hydrophilic filtering structure and the front end of the inner layer capillary;
the liquid drop sampling gun head comprises an outer layer pipeline, an inner layer capillary tube, a liquid drop sampling gun head, a negative pressure device and a liquid drop collecting gun head, wherein an oil phase is filled between the outer layer pipeline and the inner layer capillary tube, the far end of the outer layer pipeline is connected with an oil storage device, the far end of the inner layer capillary tube is connected with the negative pressure device, and when the front end of the liquid drop sampling gun head is immersed in a water phase sample to be collected, the water phase sample can be collected into the inner layer capillary tube in a liquid drop mode wrapped by the oil phase and is continuously conveyed from the front end to the rear end.
2. A novel liquid droplet sampling gun head according to claim 1, in which the structure of the sampling gun head, with the exception of the hydrophilic filter structure, is integrally formed by 3D printing.
3. A novel liquid droplet sampling gun head according to claim 1, in which the holder is a sheet of material having a through hole, the inner capillary tube passing through the through hole and being fixedly connected thereto at the through hole by the holder, at least a portion of the outer periphery of the sheet of material being connected to the outer tube.
4. A novel liquid drop sampling gun head according to claim 1, wherein the hydrophilic filter structure is held 1-3 mm from the front end of the inner capillary tube.
5. The novel liquid drop sampling gun head according to claim 1, wherein the hydrophilic filtering structure is fixed to the front end of the outer-layer pipeline by ultraviolet light curing glue.
6. A sampling system comprising a novel liquid droplet sampling gun head according to any one of claims 1 to 5, comprising: the device comprises a liquid drop sampling gun head, an analysis device, a collecting bottle, negative pressure equipment and an oil storage bottle, wherein the liquid drop sampling gun head, the analysis device, the collecting bottle and the negative pressure equipment are sequentially connected through a pipeline; wherein, the analysis device is connected with the inner layer capillary of the sampling gun head.
7. The sampling system of claim 6, further comprising: an oil phase input pipeline led in by a joint beside a sample inlet pipeline between the sampling gun head and the input end of the analysis device, and a reagent input pipeline led in by the oil phase input pipeline by passing through another joint, so that a detection reagent droplet wrapped by the oil phase enters the sample inlet pipeline and is mixed with the sample droplet on line.
8. A novel liquid droplet sampling gun head according to claim 6, in which the negative pressure device is selected from: any one of a negative pressure pump, a syringe and an automatic pipetting workstation.
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| CN202122001343.8U CN215866747U (en) | 2021-08-24 | 2021-08-24 | Novel liquid drop sampling gun head and sampling system comprising same |
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| CN202122001343.8U CN215866747U (en) | 2021-08-24 | 2021-08-24 | Novel liquid drop sampling gun head and sampling system comprising same |
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