CN215855774U

CN215855774U - Microelectrode drawing instrument

Info

Publication number: CN215855774U
Application number: CN202121669953.9U
Authority: CN
Inventors: 欧阳超; 阳方义; 潘雅楠
Original assignee: Rwd Life Science Co ltd
Current assignee: Rwd Life Science Co ltd
Priority date: 2021-07-22
Filing date: 2021-07-22
Publication date: 2022-02-18
Anticipated expiration: 2031-07-22

Abstract

The embodiment of the utility model provides a microelectrode drawing instrument, which comprises: a clamp fixing seat; the first clamp module is fastened on the clamp fixing seat; the second clamp module is arranged opposite to the first clamp module; and a gas ejection hole; the first clamp module comprises a first clamp seat and a first clamp seat cover, a groove is formed in the first clamp seat, a boss is arranged on the first clamp seat cover, and the groove and the boss are mutually meshed. According to the embodiment of the utility model, the first clamp module for fixing one end of the heating sheet is detachably fixed on the clamp fixing seat, the first clamp seat of the first clamp module is provided with the groove, the first clamp seat cover of the first clamp module is provided with the boss, one end of the heating sheet is only required to be placed into the groove for fixing when the glass microelectrodes with the same specification are drawn, and only the first clamp module with different groove widths is required to be replaced when the glass microelectrodes with different specifications are drawn, so that the time required for replacing the heating sheet is reduced.

Description

Microelectrode drawing instrument

Technical Field

The utility model relates to the technical field of microelectrode drawing instruments, in particular to a microelectrode drawing instrument.

Background

The glass microelectrode is a component which is clamped by a probe of a patch clamp system and is directly sealed with a cell membrane. The glass microelectrode is mainly formed by drawing different glass capillaries by controlling the parameters of the microelectrode drawing instrument, such as temperature, speed, tension, drawing time and the like.

At present, the existing microelectrode drawing instruments can be divided into vertical microelectrode drawing instruments and horizontal microelectrode drawing instruments. The horizontal microelectrode drawing instrument heats the glass capillary tube by a large current through a heating wire or a heating sheet until the glass capillary tube reaches the softening point, applies a certain pulling force to two ends of the glass capillary tube which is horizontally placed to thin the softened glass capillary tube, detects the speed jump point through a speed sensor when the glass capillary tube reaches a specific position, and then sprays dry gas to cool the softened glass capillary tube so as to re-solidify the glass capillary tube to prepare the glass microelectrode. So as to realize single or multiple drawing.

The horizontal microelectrode drawing instrument is mainly characterized in that the parameter configuration is flexible, two symmetrical glass microelectrodes can be formed by one-time drawing, and the use efficiency of the glass capillary tube is high; the disadvantage is the high relative position requirements for the heating wire or heating plate, the glass capillary, the gas injection orifice. The heating wire or the heating sheet is required to be positioned right above the air jet hole, the glass capillary tube vertically penetrates through the central position surrounded by the heating wire or the heating sheet and is just positioned right above the air jet hole, so that a pair of glass microelectrodes with very high symmetry can be drawn, otherwise, two tips of the two drawn glass microelectrodes can be long and short, and the use requirements are not met. The glass microelectrodes with different specifications correspond to heating wires or heating sheets with different specifications, and the heating wires or the heating sheets with different specifications need to be replaced when the glass microelectrodes with different specifications are drawn. Therefore, it is necessary to take a lot of time to adjust the positions of the respective parts to be optimal when the heating wire or the heating sheet is replaced to draw the glass micro-electrode satisfying the use requirement.

SUMMERY OF THE UTILITY MODEL

The embodiment of the utility model provides a rotor, and aims to solve the problem that a large amount of time is needed to adjust the positions of all parts when a heating sheet is replaced in the prior art.

In a first aspect, there is provided a microelectrode drawing instrument, comprising:

a clamp fixing seat (1);

a first clamp module (2) fastened on the clamp fixing seat;

a second gripper module (3) arranged opposite to the first gripper module (2); and

an air injection hole (4) arranged between the first clamp module (2) and the second clamp module (3);

the first clamp module (2) comprises a first clamp seat (21) and a first clamp seat cover (22), a groove (211) is formed in the first clamp seat (21), a boss (221) is formed in the first clamp seat cover (22), and the groove (211) and the boss (221) are meshed with each other; the second gripper module (3) comprises a second gripper seat (31) and a second gripper seat cover (32).

According to the embodiment of the utility model, the first clamp module for fixing one end of the heating sheet is detachably fixed on the clamp fixing seat, the first clamp seat of the first clamp module is provided with the groove, correspondingly, the first clamp seat cover of the first clamp module is provided with the boss, and the groove and the boss are mutually occluded.

Drawings

The foregoing and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:

FIG. 1 is a partial schematic view of a microelectrode drawing apparatus provided in an embodiment of the present invention;

FIG. 2 is a side view of a microelectrode drawing apparatus provided in an embodiment of the present invention;

FIG. 3 is a schematic illustration of two different gauge first clamp modules provided by an embodiment of the present invention;

FIG. 4 is a schematic view of three different sized heater strips according to an embodiment of the present invention;

fig. 5 is a partial schematic view of a tie rod provided by an embodiment of the present invention.

Detailed Description

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar modules or modules having the same or similar functionality throughout. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention. On the contrary, the embodiments of the utility model include all changes, modifications and equivalents coming within the spirit and terms of the claims appended hereto.

In the laboratory environment of colleges and universities, research institutions, hospitals and reproductive institutions, laboratory personnel need to use glass microelectrodes for patch clamp experiments and microinjection operations. The glass microelectrode is formed by drawing a glass capillary tube with two uniform ends and the diameter of less than 4mm, one end of the glass microelectrode is a tip, the diameter of the tip is generally less than 3um, and the longest length of the cone cannot exceed 2 cm. The specification of the heating sheet used in the drawing has a direct influence on the specification of the finally obtained glass microelectrode. The widths of the heating sheets are different, and the diameter of the tip of the drawn glass micro-electrode, the length of the taper, and the resistance value after filling the liquid in the glass micro-electrode are different. In general, wider heating sheets are more suitable for drawing thin and long glass microelectrodes with sharp tips.

According to the embodiment of the utility model, the first clamp module for fixing one end of the heating sheet is detachably fixed on the clamp fixing seat, the first clamp seat of the first clamp module is provided with the groove, correspondingly, the first clamp seat cover of the first clamp module is provided with the boss, and the groove and the boss are mutually occluded, so that when the glass microelectrodes with the same specification are drawn, one end of the heating sheet is only required to be placed into the groove for fixing, when the glass microelectrodes with different specifications are drawn, only the first clamp module with different groove widths is required to be replaced, and then one end of the heating sheet is placed into the groove for fixing, so that the time required for replacing the heating sheet is reduced, the efficiency of replacing the heating sheet is improved, and the accuracy of installing the heating sheet is improved.

FIG. 1 is a partial schematic view of a microelectrode drawing apparatus provided in an embodiment of the present invention. As shown in FIG. 1, the microelectrode drawing instrument comprises a clamp fixing seat 1, a first clamp module 2, a second clamp module 3, a gas orifice 4 and a third fastening screw 5. The third fastening screw 5 fastens the first clamp module 2 on the clamp fixing seat 1, and the modular design enables the first clamp module 2 to be detached when needed, and another clamp module is replaced, so that different requirements can be met, and the replacement efficiency is high. In an embodiment of the utility model, the first clamp module 2 comprises a first clamp base 21, a first clamp base cover 22 and a first fastening screw 23, the first fastening screw 23 fastening the first clamp base cover 22 to the first clamp base 21. The second clamp module 3 comprises a second clamp base 31, a second clamp base cover 32 and a second fastening screw 33, the second fastening screw 33 fastening the second clamp base cover 32 to the second clamp base 31. The first clamp module 2 and the second clamp module 3 are oppositely arranged and used for fixing the heating plate A.

FIG. 2 is a side view of a micro-electrode drawing apparatus provided in an embodiment of the present invention. The surrounding part of the heating plate A is square, two ends of the heating plate A extend outwards from two sides of the square and are respectively clamped and fixed by the first clamp module 2 and the second clamp module 3. The glass capillary tube B passes vertically from the center position of the square. And an air injection hole 4 is arranged between the first clamp module 2 and the second clamp module 3 and right below the center of a square surrounded by the heating sheets A. When the glass capillary tube needs to be cooled in the drawing process, dry normal-temperature gas is ejected from the gas ejecting holes 4, so that the softened glass capillary tube is solidified again.

In order to reduce the time for adjusting the positions of the parts when the heating sheet is replaced, in order to draw the glass microelectrode meeting the use requirements, the requirements on the relative positions of the heating sheet A, the glass capillary tube B and the gas injection hole 4 are high, as one embodiment of the utility model, the first clamp seat 21 is provided with the groove 211, the first clamp seat cover 22 is provided with the boss 221, and the groove 211 and the boss 221 are mutually meshed. Figure 3 is a schematic illustration of two different specification first gripper modules provided by an embodiment of the present invention. FIG. 4 is a schematic diagram of three different sizes of heat patch according to an embodiment of the present invention. The difference is that the widths of both ends of the heating plate a are different, the width of the groove 211 formed in the first clamp holder 21 is different, and the width of the boss 221 formed in the first clamp holder cover 22 is different. And selecting the adaptive heating plate A and the first clamp module, putting one end of the heating plate A into the groove 211, fixing the heating plate A by the groove 211 and the boss 221, and fixing the other end of the heating plate A by the second clamp module 3. The design of the mutually meshed grooves and the bosses positions the heating plate, reduces the time spent in the process of replacing the heating plate, improves the efficiency of replacing the heating plate, and improves the accuracy of installing the heating plate.

As shown in FIG. 1, in the embodiment of the present invention, the microelectrode drawing instrument further comprises two sets of W-shaped pulleys 6 and pull rods 7 symmetrically disposed on both sides of the connection line between the first clamping module 2 and the second clamping module 3. For each side, the group of W-shaped pulleys 6 comprises 3W-shaped pulleys, and the V shapes are distributed on the upper side and the lower side of the group of pull rods 7 on the same side. Two W-shaped pulleys are arranged below the pull rod 7, and a W-shaped pulley is arranged above the pull rod 7 to guide the pull rod 7. Fig. 5 is a partial schematic view of a tie rod provided by an embodiment of the present invention. As shown in fig. 5, each set of tie rods 7 is provided with a V-shaped groove 71. The glass capillary tube B is fixed to the V-groove 71.

Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.

Claims

1. A microelectrode drawing instrument, comprising:

a clamp fixing seat (1);

a first clamp module (2) fastened on the clamp fixing seat;

an air vent (4) disposed between the first gripper module (2) and the second gripper module (3);

2. Microelectrode drawing instrument according to claim 1, wherein one end of the heat patch (A) is fixed by said recess (211) and said boss (221) and the other end is fixed by said second clamping module (3).

3. Microelectrode drawing instrument according to claim 1, characterized in that the first clamp module (2) further comprises a first fastening screw (23), the first fastening screw (23) fastening the first clamp seat cover (22) to the first clamp seat (21).

4. Microelectrode drawing instrument according to claim 1, characterized in that the second clamp module (3) further comprises a second fastening screw (33), the second fastening screw (33) fastening the second clamp seat cover (32) to the second clamp seat (31).

5. Microelectrode drawing instrument according to claim 1, further comprising a third fastening screw (5), wherein the third fastening screw (5) fastens the first clamp module (2) to the clamp holder (1).

6. The microelectrode drawing instrument according to claim 1, further comprising two sets of W-shaped pulleys (6) and pull rods (7) symmetrically arranged on both sides of a connecting line of the first clamping module (2) and the second clamping module (3).

7. Microelectrode drawing machine according to claim 6, wherein each set of W-shaped pulleys (6) comprises 3W-shaped pulleys, and the V-shaped pulleys are arranged on the upper and lower sides of each set of draw bars (7).

8. Microelectrode drawing instrument according to claim 6, wherein each set of draw bars (7) is provided with a V-shaped groove (71).

9. Microelectrode drawing instrument according to claim 8, characterized in that a glass capillary (B) is fixed to said V-groove (71).