CN215841737U

CN215841737U - Chromatographic separation system

Info

Publication number: CN215841737U
Application number: CN202122423598.3U
Authority: CN
Inventors: 杨黎; 孙永康
Original assignee: ZIBO INSTITUTE FOR FOOD AND DRUG CONTROL
Current assignee: ZIBO INSTITUTE FOR FOOD AND DRUG CONTROL
Priority date: 2021-10-09
Filing date: 2021-10-09
Publication date: 2022-02-18
Anticipated expiration: 2031-10-09

Abstract

The utility model provides a chromatographic separation system, comprising: a six-way valve; the input end of the first chromatographic column is connected with the second port of the six-way valve, and the output end of the first chromatographic column is connected with the fifth port of the six-way valve; the input end of the second chromatographic column is connected with the sixth port of the six-way valve, and the output end of the second chromatographic column is connected with the third port of the six-way valve; the six-way valve is switchable between a first state and a second state. According to the chromatographic separation system disclosed by the utility model, a special column temperature box is not needed, the flow rate is not needed to be too low, and the effect of theoretically infinite series connection of chromatographic columns can be achieved.

Description

Chromatographic separation system

Technical Field

The utility model relates to the technical field of compound separation, in particular to a chromatographic separation system.

Background

Effective separation of compounds is the basis of compound detection, and the separation degree between compounds is an important parameter of chromatography, so that qualitative and quantitative operations are meaningful only on the basis of compound separation. In the actual working process, compounds with close properties are difficult to separate, and on the premise that the common chromatography regulation is ineffective, the separation degree is increased by generally adopting a chromatographic column series method to prolong the length of a chromatographic column, but the method has the following defects:

1. the analysis cost is increased, the prices of the chromatographic columns are 2000-30000 yuan, and the hardware cost is increased by connecting a plurality of chromatographic columns in series; 2. the column pressure increases, resulting in system failure. The more chromatographic columns are connected in series, the higher the system pressure is, and the system protection alarm can stop running; 3. the retention time is too long, which seriously affects the detection efficiency. In order to stabilize the system, on the premise of not increasing the column pressure, the flow rate is reduced to be the first choice, under the influence of factors such as lengthening of a chromatographic column, reduction of the flow rate and the like, the retention time of a compound is greatly prolonged, the detection efficiency is influenced, in addition, the peak broadening is serious due to the prolonging of the time, and the sensitivity is greatly reduced; 4. the operation is inconvenient, a plurality of chromatographic columns are connected in series, so that more connectors are needed, and the liquid leakage probability is increased; in addition, the constant temperature of a large number of chromatographic columns requires a larger column temperature box, and the current mainstream instrument configuration is difficult to meet the requirements.

SUMMERY OF THE UTILITY MODEL

The present invention is directed to solving, at least to some extent, one of the technical problems in the related art.

Therefore, an object of the present invention is to provide a chromatographic separation system that can achieve the effect of theoretically infinite series connection of chromatographic columns without requiring a special column oven and without requiring too low a flow rate.

A chromatographic separation system according to the present invention comprises:

a six-way valve; the six-way valve has a first port, a second port, a third port, a fourth port, a fifth port, and a sixth port;

the input end of the first chromatographic column is connected with the second port of the six-way valve, and the output end of the first chromatographic column is connected with the fifth port of the six-way valve;

the input end of the second chromatographic column is connected with the sixth port of the six-way valve, and the output end of the second chromatographic column is connected with the third port of the six-way valve;

the six-way valve is switchable between a first state and a second state.

Advantageously, when the six-way valve is in the first state, the first port is connected to the second port, the third port is connected to the fourth port, and the fifth port is connected to the sixth port.

Advantageously, when the six-way valve is in the second state, the first port is connected to the sixth port, the second port is connected to the third port, and the fourth port is connected to the fifth port.

Advantageously, the six-way valve is an electromagnetic six-way valve.

Advantageously, the chromatographic separation system further comprises a pump, the output of which is connected to the first port of the six-way valve.

Advantageously, the chromatographic separation system further comprises a detector connected to the fourth port of the six-way valve.

Advantageously, the chromatographic separation system further comprises a data terminal connected to the detector.

Advantageously, the data terminal is a desktop computer, a laptop computer, a tablet computer or a smartphone.

Additional aspects and advantages of the utility model will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the utility model.

Drawings

FIG. 1 is a schematic view of a chromatographic separation system according to one embodiment of the present invention with a six-way valve in a first state;

FIG. 2 is a schematic view of a chromatographic separation system in accordance with another embodiment of the utility model with the six-way valve in the second state.

Detailed Description

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative and intended to be illustrative of the utility model and are not to be construed as limiting the utility model.

A chromatographic separation system according to the present invention is described in detail below with reference to the accompanying drawings.

As shown in fig. 1 and 2, a chromatographic separation system according to the present invention is characterized by comprising: six-way valve 200, first chromatography column 300, second chromatography column 400.

Specifically, six-way valve 200 may be an electromagnetic six-way valve. The first port 201 of the six-way valve 200 is used for compound input. For example, the chromatographic separation system further comprises a pump 100, the output of the pump 100 being connected to the first port 201 of the six-way valve 200 for delivering the compounds to be separated. The fourth port 204 of the six-way valve 200 is used for the output of the separated compound. For example, the chromatographic separation system further comprises a detector 500, and the detector 500 is connected to the fourth port 204 of the six-way valve 200 for detecting the separated compounds.

The input of the first chromatography column 300 is connected to the second port 202 of the six-way valve 200 and the output of the first chromatography column 300 is connected to the fifth port 205 of the six-way valve 200.

An input of second chromatography column 400 is connected to sixth port 206 of six-way valve 200 and an output of second chromatography column 400 is connected to third port 203 of six-way valve 200.

Therein, the six-way valve 200 is switchable between a first state and a second state.

When the six-way valve 200 is in the first state, as shown in fig. 1, the first port 201 is connected to the second port 202, the third port 203 is connected to the fourth port 204, and the fifth port 205 is connected to the sixth port 206. At this time, the compound is transported by the pump 100, and passes through the first port 201, the second port 202, the input end of the first chromatography column 300, the output end of the first chromatography column 300, the fifth port 205, the sixth port 206, the input end of the second chromatography column 400, the output end of the second chromatography column 400, and the third port 203 in this order, and the compound is separated, and reaches the detector 500 from the fourth port 204 for detection.

When the six-way valve 200 is in the second state, as shown in fig. 2, the first port 201 is connected to the sixth port 206, the second port 202 is connected to the third port 203, and the fourth port 204 is connected to the fifth port 205. At this time, the compound is transported by the pump 100 to pass through the first port 201, the sixth port 206, the input end of the second chromatography column 400, the output end of the second chromatography column 400, the third port 203, the second port 202, the input end of the first chromatography column 300, the output end of the first chromatography column 300, and the fifth port 205 in this order, and the compound is separated and reaches the detector 500 from the fourth port 204 to be detected.

It should be noted that the arrows in fig. 1 and 2 indicate the flowing direction of the mobile phase, which is understood by those skilled in the art.

That is, in the first state shown in fig. 1, the mobile phase passes through the first chromatography column 300, then passes through the second chromatography column 400, and then enters the detector 500. In the second state, shown in fig. 2, the mobile phase passes through the second chromatography column 400, then through the second chromatography column 300, and then into the detector 500.

The separation effect of infinite chromatographic column series connection is achieved by the following operations of the compounds: when the compound is in the first state, the compound is separated by the first chromatographic column 300 and reaches the second chromatographic column 400, at this time, the six-way valve 200 is switched to the second state, the compound reaches the first chromatographic column 300 after the second chromatographic column 400 is separated, then the six-way valve 200 is switched to the first state, the compound continues to be separated by the first chromatographic column 300 and reaches the second chromatographic column 400, then the six-way valve 200 is switched to the second state, the compound reaches the first chromatographic column 300 after the second chromatographic column 400 is separated, and the process is circulated until each compound is completely separated, and finally the compound enters the detector 500.

According to a specific example of the present invention, the chromatographic separation system further includes a data terminal 600, and the data terminal 600 is connected to the detector 500. Advantageously, the data terminal 600 is a desktop computer, a laptop computer, a tablet computer or a smartphone. Thus, when the separated compounds enter the detector 500, the signals are processed, and a chromatogram can be obtained at the data terminal 600.

According to the chromatographic separation system, the six-way valve 200 is arranged, the six-way valve 200 can be switched between the first state and the second state, and for the separation of various compounds difficult to separate, the compounds are subjected to reciprocating circulating separation between the first chromatographic column 300 and the second chromatographic column 400 through the switching of the six-way valve 200, so that the circulating separation of the compounds which are not separated in the 2 groups of chromatographic columns is realized until the final separation purpose is achieved.

In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the utility model and to simplify the description, and are not intended to indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and are therefore not to be considered limiting of the utility model.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.

In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

In the present invention, unless otherwise expressly stated or limited, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through an intermediate. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the utility model. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.

Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.

Claims

1. A chromatographic separation system, comprising:

the six-way valve is switchable between a first state and a second state.

2. The chromatographic separation system of claim 1, wherein the first port is connected to the second port, the third port is connected to the fourth port, and the fifth port is connected to the sixth port when the six-way valve is in the first state.

3. The chromatographic separation system of claim 1, wherein the first port is connected to the sixth port, the second port is connected to the third port, and the fourth port is connected to the fifth port when the six-way valve is in the second state.

4. The chromatographic separation system of any of claims 1-3, wherein the six-way valve is an electromagnetic six-way valve.

5. The chromatographic separation system of claim 4, further comprising a pump, an output of the pump being connected to the first port of the six-way valve.

6. The chromatographic separation system of claim 4, further comprising a detector connected to the fourth port of the six-way valve.

7. The chromatographic separation system of claim 6, further comprising a data terminal connected to the detector.

8. The chromatographic separation system of claim 7, wherein the data terminal is a desktop computer, a laptop computer, a tablet computer, or a smartphone.