CN215840481U - Liposome medicine carrying equipment - Google Patents
Liposome medicine carrying equipment Download PDFInfo
- Publication number
- CN215840481U CN215840481U CN202120863111.0U CN202120863111U CN215840481U CN 215840481 U CN215840481 U CN 215840481U CN 202120863111 U CN202120863111 U CN 202120863111U CN 215840481 U CN215840481 U CN 215840481U
- Authority
- CN
- China
- Prior art keywords
- gas
- collecting hood
- evaporator
- sealing ring
- liposome
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000002502 liposome Substances 0.000 title claims abstract description 24
- 239000003814 drug Substances 0.000 title claims abstract description 19
- 238000007789 sealing Methods 0.000 claims abstract description 16
- 229940079593 drug Drugs 0.000 claims description 12
- 238000011068 loading method Methods 0.000 claims description 9
- 239000011521 glass Substances 0.000 claims description 3
- 239000003960 organic solvent Substances 0.000 description 11
- 239000000839 emulsion Substances 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 5
- 210000003022 colostrum Anatomy 0.000 description 5
- 235000021277 colostrum Nutrition 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 150000003904 phospholipids Chemical class 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- 239000008346 aqueous phase Substances 0.000 description 3
- 238000004945 emulsification Methods 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- RXUWDKBZZLIASQ-UHFFFAOYSA-N Puerarin Natural products OCC1OC(Oc2c(O)cc(O)c3C(=O)C(=COc23)c4ccc(O)cc4)C(O)C(O)C1O RXUWDKBZZLIASQ-UHFFFAOYSA-N 0.000 description 2
- 230000002209 hydrophobic effect Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- HKEAFJYKMMKDOR-VPRICQMDSA-N puerarin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1C1=C(O)C=CC(C2=O)=C1OC=C2C1=CC=C(O)C=C1 HKEAFJYKMMKDOR-VPRICQMDSA-N 0.000 description 2
- 239000000232 Lipid Bilayer Substances 0.000 description 1
- 239000000823 artificial membrane Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 150000003408 sphingolipids Chemical class 0.000 description 1
Images
Landscapes
- Medicinal Preparation (AREA)
Abstract
The utility model discloses liposome medicine carrying equipment which comprises a magnetic stirrer, an evaporator, a sealing ring, a gas collecting hood, a gas guide tube and a buffer bottle.
Description
Technical Field
The utility model relates to the field of pharmacy, in particular to a liposome drug loading device.
Background
Liposomes (liposomes) are an artificial membrane. The hydrophilic head of phospholipid molecule is inserted into water, the hydrophobic tail of liposome extends to air, and spherical liposome with double-layer lipid molecule with diameter of 25-1000nm is formed after stirring. Liposomes can be used for transgenosis, or for preparing drugs, and the drugs are delivered into the cell interior according to the biological definition by utilizing the characteristic that the liposomes can be fused with cell membranes: when amphiphilic molecules such as phospholipids and sphingolipids are dispersed in an aqueous phase, the hydrophobic tails of the molecules tend to cluster together, avoiding the aqueous phase, while the hydrophilic heads are exposed to the aqueous phase, forming closed vesicles with a bilayer structure, known as liposomes.
Pharmaceutically defined liposomes (liposome): refers to a microvesicle formed by encapsulating a drug in a lipid bilayer. The commonly used liposome drug-loading method includes a film dispersion method, a multiple emulsion method, an injection method and the like. The multiple emulsion method is that step 1, phospholipid is dissolved in organic solvent, added with solution of drug to be encapsulated, emulsified to obtain W/O colostrum, step 2, the colostrum is added into water with certain volume and mixed, W/O/W is emulsified to obtain emulsion, and then the organic solvent is removed at certain temperature to obtain liposome, thus completing drug loading. The temperature in the organic solvent removing process has great influence on the particle size of the liposome, and the lower temperature is favorable for preparing the liposome with smaller particle size.
SUMMERY OF THE UTILITY MODEL
The utility model provides a liposome drug-loading device, which is used for carrying out liposome drug loading, is simple to operate and can quickly remove an organic solvent at room temperature.
The technical scheme adopted by the utility model for solving the technical problems is as follows:
a liposome medicine carrying device comprises a magnetic stirrer, an evaporator, a sealing ring, a gas collecting hood, a gas guide tube and a buffer bottle; the upper opening of the gas-collecting hood and the gas leading-in opening of the buffer bottle can be communicated with each other through a gas guide pipe; the evaporator is cylindrical; the gas-collecting hood is conical.
The outer diameter of the sealing ring is larger than that of the evaporator; the inner diameter of the sealing ring is smaller than the maximum inner diameter of the gas-collecting hood.
The evaporator and the gas collecting hood are made of glass; the seal ring is made of oil-resistant rubber.
The operation process of the utility model is that a certain amount of phospholipid is dissolved in organic solvent (such as chloroform and ether), and added with solution (such as puerarin water solution) of drug to be encapsulated, and emulsified to obtain W/O colostrum. Adding water with the volume 10 times that of the primary emulsion into an evaporator, starting a magnetic stirrer, placing a sealing ring on the evaporator, placing a gas-collecting hood on the sealing ring, dropwise adding the primary emulsion from the upper opening of the gas-collecting hood, and adjusting the stirring speed to mix and emulsify the primary emulsion. After the dropping of the primary emulsion is finished, the upper opening of the gas collecting hood is communicated with the gas leading-in opening of the buffer bottle through the gas guide tube, the gas leading-out opening of the buffer bottle is connected to the air pump for air suction, the organic solvent is removed, the liposome is formed, and the drug loading is completed. The removal rate of the organic solvent can be adjusted by adjusting the power of the suction pump.
The method has the advantages of capability of quickly removing the organic solvent at room temperature, controllable removal speed and simple operation.
Drawings
The utility model is further illustrated with reference to the following figures and examples.
FIG. 1 is a schematic diagram of the apparatus of the present invention.
In the figure: 1. a magnetic stirrer; 2. an evaporator; 3. a seal ring; 4. a gas-collecting hood; 41. an upper opening; 5. an air duct; 6. a buffer bottle; 61. a gas inlet; 62. a gas outlet.
Detailed Description
In order to make the technical means, innovative features, objectives and effects of the present invention apparent, the present invention will be further described with reference to the following detailed drawings.
Referring to fig. 1, the liposome medicine carrying device comprises a magnetic stirrer (1), an evaporator (2), a sealing ring (3), a gas collecting hood (4), a gas guide tube (5) and a buffer bottle (6); the upper opening (41) of the gas collecting hood (4) and the gas introducing port (61) of the buffer bottle (6) can be communicated with each other through a gas guide pipe (5); the evaporator (2) is cylindrical; the gas-collecting hood (4) is conical.
The outer diameter of the sealing ring (3) is larger than that of the evaporator (2); the inner diameter of the sealing ring (3) is smaller than the maximum inner diameter of the gas-collecting hood (4).
The evaporator (2) and the gas collecting hood (4) are made of glass; the sealing ring (3) is made of oil-resistant rubber.
When the equipment works, a certain amount of phospholipid is dissolved in an organic solvent (such as chloroform and diethyl ether), a solution (such as puerarin water solution) to be coated with a medicament is added, and the W/O colostrum is obtained by emulsification. Adding water with the volume 10 times that of the primary emulsion into an evaporator (2), starting a magnetic stirrer (1), placing a sealing ring (3) on the evaporator (2), placing a gas collecting hood (4) on the sealing ring (3), dripping the primary emulsion from an upper opening (41) of the gas collecting hood (4), and adjusting the stirring speed to mix and emulsify the primary emulsion. After the colostrum is dripped, an upper opening (41) of the gas collecting hood (4) is communicated with a gas introducing opening (61) of the buffer bottle (6) through a gas guide tube (5), a gas leading-out opening (62) of the buffer bottle (6) is connected to a suction pump for sucking air, removing the organic solvent, forming the liposome and finishing the drug loading. The removal rate of the organic solvent can be adjusted by adjusting the power of the suction pump.
Claims (3)
1. A liposome medicine carrying equipment is characterized in that: comprises a magnetic stirrer (1), an evaporator (2), a sealing ring (3), a gas-collecting hood (4), a gas-guide tube (5) and a buffer bottle (6); the upper opening (41) of the gas collecting hood (4) and the gas introducing port (61) of the buffer bottle (6) can be communicated with each other through a gas guide pipe (5); the evaporator (2) is cylindrical; the gas-collecting hood (4) is conical.
2. The liposomal drug loading apparatus of claim 1, wherein: the outer diameter of the sealing ring (3) is larger than that of the evaporator (2); the inner diameter of the sealing ring (3) is smaller than the maximum inner diameter of the gas-collecting hood (4).
3. The liposomal drug loading apparatus of claim 1, wherein: the evaporator (2) and the gas collecting hood (4) are made of glass; the sealing ring (3) is made of oil-resistant rubber.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120863111.0U CN215840481U (en) | 2021-04-25 | 2021-04-25 | Liposome medicine carrying equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120863111.0U CN215840481U (en) | 2021-04-25 | 2021-04-25 | Liposome medicine carrying equipment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215840481U true CN215840481U (en) | 2022-02-18 |
Family
ID=80315698
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120863111.0U Expired - Fee Related CN215840481U (en) | 2021-04-25 | 2021-04-25 | Liposome medicine carrying equipment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215840481U (en) |
-
2021
- 2021-04-25 CN CN202120863111.0U patent/CN215840481U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20220218 |