CN215828769U - Sampling device suitable for biological sample in ancient calligraphy and painting of ancient book archives - Google Patents
Sampling device suitable for biological sample in ancient calligraphy and painting of ancient book archives Download PDFInfo
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- CN215828769U CN215828769U CN202120404622.6U CN202120404622U CN215828769U CN 215828769 U CN215828769 U CN 215828769U CN 202120404622 U CN202120404622 U CN 202120404622U CN 215828769 U CN215828769 U CN 215828769U
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Abstract
The utility model discloses a sampling device suitable for biological samples in ancient scripts and paintings of ancient book archives. The device consists of an exhaust fan, a collecting device and a sampling device; the exhaust fan is connected with the exhaust pipe of the collecting device through a hose, and the collecting pipe of the collecting device is connected with the sampling pipe of the sampling device through a hose. The utility model is a convenient and effective system sampling device without damage, and the sampling device can scientifically collect microorganisms or/and nucleic acid samples from paper articles including ancient books, archives, ancient calligraphy and painting and the like for scientific research and protection work.
Description
Technical Field
The utility model relates to a sampling device, in particular to a sampling device applied to biological samples on paper articles such as ancient books, archives, ancient calligraphy and painting.
Background
Ancient books are the abbreviation of ancient literature classic in China. The ancient book definition at present mainly refers to books written and printed before 1912 in the form of Chinese classical frames, including Chinese and minority nationality characters. Ancient books are inevitably damaged for various reasons of preservation and use, wherein damage caused by microbial contamination accounts for more than four folds of ancient books. The microorganisms are microorganisms which are difficult to observe by naked eyes, and the main microorganisms which have strong destructive effect on paper such as ancient books, calligraphy and painting are moulds and partial bacteria. Mould is not a taxonomic term, it is a generic term for fungi that form branched hyphae, meaning "mildewed fungi," which belong in the taxonomic class to the various subgenus of the phylum mycotina. In damp and warm places, a number of macroscopic villous, flocculent or arachnoid colonies, i.e. molds, can grow on the articles. The mould is wide in distribution and strong in reproductive capacity, can not be found by naked eyes when being in a spore state, has long dormancy period and strong stress resistance, can not be reproduced temporarily when the conditions are not suitable, can preserve activity for a long time, and is full of spores of the mould in the air. The bacteria are a type of prokaryotic microorganisms, are a type of prokaryotic organisms which are thin and short in shape and simple in structure and are propagated mostly in a binary fission mode, have the diameter of 0.5-5 mu m, and are organisms which are most widely distributed in the nature and have the largest individual number. Spores are dormant bodies of bacteria and have extremely strong resistance to adverse environments. The small and light spores can also drift around with the wind, fall into a proper environment and can germinate into bacteria. The rapid proliferation and sporulation properties of bacteria make them almost ubiquitous. The paper harms the growth of microorganisms and the formation of bacterial plaque, not only can influence the visual readability effect of the paper, but also can degrade paper components to cause irreversible lesion, so that the strength of the paper is reduced, the service life of the paper is shortened, and even a large number of metabolites are bonded to form a paper brick which cannot be repaired. The ancient books can not be stored in vacuum, so that the ancient books face the risk of being corroded by microorganisms such as mold and the like all the time, the initial pollution is invisible to the naked eyes and cannot be found easily, and once the ancient books are found, the ancient books are damaged. To prevent and treat the damage of microorganisms such as mold and the like to ancient books, the cause of scientific cognitive damage is firstly required to reveal which kind or kinds of microorganisms influence the ancient books, so that microorganisms or/and nucleic acid samples can be scientifically, effectively and safely collected from the ancient books, and a sample is provided for scientifically analyzing the invasion of the ancient books by the microorganisms, so that the method has important practical significance.
The current conventional acquisition process is as follows: after the sampling position is determined, wetting the wetted polyester fiber swab by using a wetting solution (0.15mol/L NaCl and Tween with the volume fraction of 0.1%), and then performing unidirectional soft wiping for 20 times within the range of 2cm multiplied by 2cm in a target area, so as not to damage the surface of the paper; the method for collecting the air-settled sample as a control comprises the following steps: when sampling is started, the polyester fiber swab is dipped in wetting liquid and then stands on a sampling worktable, and the swab is collected in other sample processing modes after sampling is finished. It can be seen that the traditional sampling mode has the following defects that the sampling process has a friction process on ancient books, chemical reagents are used, the sampling efficiency is low, and the human error in the sampling process is large.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a convenient, nondestructive and effective system sampling device, which can scientifically collect microorganism or/and nucleic acid samples from paper articles including ancient scripts and paintings of ancient book archives for scientific research and protection.
The technical scheme adopted by the utility model is as follows: a sampling device suitable for biological samples in ancient scripts and paintings of ancient book archives comprises an exhaust fan, a collecting device and a sampling device; the exhaust fan is connected with the exhaust pipe of the collecting device through a hose, and the collecting pipe of the collecting device is connected with the sampling pipe of the sampling device through a hose.
Furthermore, foretell a sampling device suitable for biological sample in ancient book archives ancient calligraphy and painting, collection device is the filter membrane formula collection device, the filter membrane formula collection device includes that the toper pushes down body, filter membrane and toper, pushes down to be connected with the exhaust column on the toper, be connected with the collecting pipe on the toper pushes down the body, and the filter membrane is arranged in toper and is pushed down between the body to push down body, filter membrane and toper through the bolt with toper and push down the body fixed together.
Furthermore, the above-mentioned sampling device suitable for biological sample in ancient calligraphy and painting of ancient book archives is equipped with sealing washer 208 between the toper body of pushing up and the toper body of pushing down.
Further, foretell a sampling device suitable for biological sample in ancient book archives ancient calligraphy and painting, collection device is culture medium formula collection device, culture medium formula collection device includes that bottle and a plurality of load have the support frame of solid culture medium, and the both ends of bottle are connected with exhaust column and collecting pipe respectively.
Further, foretell a sampling device suitable for biological sample among ancient book archives ancient calligraphy and painting, sampling device is all-round sampling device, all-round sampling device includes the sampling body and supporter, and the system has sample connection I and pinhole and is connected with the sampling tube on the sampling body, and the system has air intake and round pin and links to each other with the air-supply line on the supporter, inserts in the pinhole through the round pin and will take a sample the body and the supporter is fixed together, waits to take a sample ancient book archives ancient calligraphy and painting sample and arranges in between the sampling body and the supporter.
Further, foretell a sampling device suitable for biological sample among ancient calligraphy and painting of ancient book archives, the outer fringe of sample connection I and air intake is less than the outer fringe of waiting to take a sample ancient book archives ancient calligraphy and painting sample.
Furthermore, foretell a sampling device suitable for biological sample in ancient book archives ancient calligraphy and painting, sampling device is point type sampling device, point type sampling device includes main part I, and I upper end of main part is connected with the sampling tube, and the lower extreme system has sample connection II.
Further, foretell a sampling device suitable for biological sample in ancient book archives ancient calligraphy and painting, sampling device is face formula sampling device, face formula sampling device includes main part II, and II one end of main part are connected with the sampling tube, and the other end is sample connection III.
Further, foretell a sampling device suitable for biological sample in ancient book archives ancient calligraphy and painting, sampling device is line formula sampling device, line formula sampling device includes main part III, and III one end of main part is connected with the sampling tube, and the other end is slit formula sample connection IV.
The utility model has the beneficial effects that:
1. the utility model can adapt to different samples by adjusting the wind power of the exhaust fan, and provides effective power. The practical working conditions of paper cultural relics or files of different dynasties, different parts and different materials are met, and the lossless and effective sampling of the target object is fully guaranteed; the repeatability of the whole sampling process is ensured; the collection rate of microorganisms in the paper fiber is ensured; the feasibility of production and popularization is ensured.
2. The utility model can change the collecting device with the change of the target object. Different collection devices are selected according to different microorganism types, sizes and metabolites thereof, so that sampling reasonability and pertinence are guaranteed, and the most scientific and effective result is achieved.
3. The utility model can change the sampling device according to the sample part. In the process of sampling ancient books or other paper cultural relics, due to the fact that microorganisms in different positions of the sample have different stubborn degrees, such as book spines, envelopes, pages and the like, or the shapes, the areas and the like of microorganism infection traces of sampling positions, different sampling devices can be replaced to achieve the optimal sampling effect.
4. The utility model has accurate sampling and high microorganism sampling rate, and solves the problem of small sample amount and difficult capture.
5. The utility model can switch the collecting device and the sampling device at any time aiming at different targets, and has universality and specificity. The physical properties of different microorganisms are different; the biological properties are different, the viscosity is different, the generation of organisms is different after the plant cellulose is decomposed, and the damage degree of paper is different, so that the capture devices with different particle sizes are more scientific.
6. According to the utility model, different sampling devices can be replaced according to different sampling research parts.
7. The utility model is portable, can deal with different working scenes, is flexible to use and has strong applicability.
8. The utility model has simple structure, and the device fittings are easy to produce and convenient to popularize.
9. Compared with the traditional sampling mode, the sampling method has the advantages of controllable physical contact, no chemical, accurate adjustment according to actual conditions, good operation repeatability, high selectivity of different targets, high sampling rate and easy popularization of market benefits.
Drawings
Fig. 1 is a schematic structural view of embodiment 1 of the present invention.
Fig. 2 is a schematic structural diagram of embodiment 2 of the present invention.
Fig. 3 is a schematic structural view of the point sampling device of the present invention.
FIG. 4 is a schematic structural diagram of a surface sampling device according to the present invention.
Fig. 5 is a schematic structural view of the wire sampling device of the present invention.
Detailed Description
Example 1
As shown in FIG. 1, a sampling device for biological samples in ancient scripts and paintings of ancient book archives is composed of an exhaust fan 100, a collecting device 200 and a sampling device 300.
The collecting device 200 is a filter membrane type collecting device, and comprises a conical upper pressing body 203, a filter membrane 204 and a conical lower pressing body 205, wherein an exhaust pipe 201 is connected to the conical upper pressing body 203, a collecting pipe 202 is connected to the conical lower pressing body 205, the filter membrane 204 is arranged between the conical upper pressing body 203 and the conical lower pressing body 205, and the conical upper pressing body 203, the filter membrane 204 and the conical lower pressing body 205 are fixed together through bolts. In order to ensure the sealing effect, a sealing ring 208 is arranged between the conical upper pressing body 203 and the conical lower pressing body 205.
In order to obtain a comprehensive biological sample of ancient scripts and paintings of the ancient book file, the sampling device 300 adopts an all-directional sampling device. As shown in FIG. 1, the omnibearing sampling device comprises a sampling body 303 and a supporting body 308, wherein the sampling body 303 is provided with a sampling port I304 and a pin hole 305 and is connected with a sampling pipe 301, the supporting body 308 is provided with an air inlet 306 and a pin 307 and is connected with an air inlet pipe 302, the sampling body 303 and the supporting body 308 are fixed together by inserting the pin 307 into the pin hole 305, and an ancient calligraphy and painting sample 500 of an ancient book file to be sampled is arranged between the sampling body 303 and the supporting body 308. Preferably, the outer edges of the sampling port I304 and the air inlet 306 are smaller than the outer edge of the ancient calligraphy and painting sample 500 of the ancient book file to be sampled.
The working process is as follows: the ancient calligraphy and painting 500 of the ancient book file is arranged between the sampling body 303 and the supporting body 308, the sampling port I304 and the air inlet 306 correspond to the ancient calligraphy and painting 500 of the ancient book file, and the sampling body 303, the ancient calligraphy and painting sample 500 of the ancient book file and the supporting body 308 are fixed together by inserting the pin 307 into the pin hole 305. The conical upper pressing body 203, the filter membrane 204 and the conical lower pressing body 205 are fixed together by bolts. The exhaust fan 100 is started, biological samples on the ancient calligraphy and painting samples 500 of the ancient book archives are sucked out through suction, and are adsorbed on the filter membrane after passing through the sampling port I304, the sampling body 303, the sampling pipe 301, the hose 400, the collecting pipe 202 and the conical pressing body 205 in sequence. The exhaust blower was turned off and the filter was removed for analysis.
Preferably, in one embodiment, the sampling device 300 is a point sampling device for obtaining a biological sample from a point on the ancient painting of the ancient book file. As shown in FIG. 3, the point sampling device comprises a main body I309, wherein the upper end of the main body I309 is connected with a sampling tube 301, and the lower end of the main body I309 is provided with a sampling port II 310. During sampling, the sampling port II 310 is aligned with the sampling point.
Preferably, in one embodiment, in order to obtain a biological sample from a part of the ancient painting of the ancient book file, the sampling device 300 is a facial sampling device, as shown in fig. 4, the facial sampling device comprises a main body II 311, one end of the main body II 311 is connected with the sampling tube 301, and the other end is a sampling port III 312. When sampling, the sampling port III 312 is placed in the sampling part.
Preferably, in one embodiment, the sampling device 300 is an in-line sampling device for obtaining biological samples from the locations of the lines in the ancient book archives. As shown in FIG. 5, the wire sampling device comprises a main body III 313, one end of the main body III 313 is connected with a sampling tube 301, and the other end is provided with a sampling port IV 314. When sampling, the sampling port IV 314 is aligned with the binding part.
Example 2
As shown in FIG. 2, a sampling device for biological samples in ancient scripts and paintings of ancient book archives is composed of an exhaust fan 100, a collecting device 200 and a sampling device 300.
The collection device 200 is a culture medium type collection device, as shown in fig. 2, the culture medium type collection device includes a bottle 206 and a plurality of support frames 207 loaded with solid culture medium, and the support frames 207 are fixed on the inner wall of the bottle 206 in an interpenetration manner. The two ends of the bottle body 206 are respectively connected with an exhaust pipe 201 and a collecting pipe 202.
In order to obtain a comprehensive biological sample of ancient scripts and paintings of the ancient book file, the sampling device 300 adopts an all-directional sampling device. As shown in FIG. 1, the omnibearing sampling device comprises a sampling body 303 and a supporting body 308, wherein the sampling body 303 is provided with a sampling port I304 and a pin hole 305 and is connected with a sampling pipe 301, the supporting body 308 is provided with an air inlet 306 and a pin 307 and is connected with an air inlet pipe 302, the sampling body 303 and the supporting body 308 are fixed together by inserting the pin 307 into the pin hole 305, and an ancient calligraphy and painting sample 500 of an ancient book file to be sampled is arranged between the sampling body 303 and the supporting body 308. Preferably, the outer edges of the sampling port I304 and the air inlet 306 are smaller than the outer edge of the ancient calligraphy and painting sample 500 of the ancient book file to be sampled.
The working process is as follows: the ancient calligraphy and painting 500 of the ancient book file is arranged between the sampling body 303 and the supporting body 308, the sampling port I304 and the air inlet 306 correspond to the ancient calligraphy and painting 500 of the ancient book file, and the sampling body 303, the ancient calligraphy and painting sample 500 of the ancient book file and the supporting body 308 are fixed together by inserting the pin 307 into the pin hole 305. The exhaust fan 100 is started, the biological samples on the ancient calligraphy and painting samples 500 of the ancient book archives are sucked out through suction, and enter the bottle body 206 through the sampling port I304, the sampling body 303, the sampling pipe 301, the hose 400 and the collecting pipe 202 in sequence, and are adsorbed on the solid culture medium. The exhaust blower is turned off and the rack 207 is removed for analysis.
Preferably, in one embodiment, the sampling device 300 is a point sampling device for obtaining a biological sample from a point on the ancient painting of the ancient book file. As shown in FIG. 3, the point sampling device comprises a main body I309, wherein the upper end of the main body I309 is connected with a sampling tube 301, and the lower end of the main body I309 is provided with a sampling port II 310. During sampling, the sampling port II 310 is aligned with the sampling point.
Preferably, in one embodiment, in order to obtain a biological sample from a part of the ancient painting of the ancient book file, the sampling device 300 is a facial sampling device, as shown in fig. 4, the facial sampling device comprises a main body II 311, one end of the main body II 311 is connected with the sampling tube 301, and the other end is a sampling port III 312. When sampling, the sampling port III 312 is placed in the sampling part.
Preferably, in one embodiment, the sampling device 300 is an in-line sampling device for obtaining biological samples from the locations of the lines in the ancient book archives. As shown in FIG. 5, the wire sampling device comprises a main body III 313, one end of the main body III 313 is connected with a sampling tube 301, and the other end is provided with a sampling port IV 314. When sampling, the sampling port IV 314 is aligned with the binding part.
Claims (7)
1. A sampling device suitable for biological samples in ancient scripts and paintings of ancient book archives is characterized by comprising an exhaust fan (100), a collecting device (200) and a sampling device (300); the exhaust fan (100) is connected with an exhaust pipe (201) of the collecting device (200) through a hose (400), and a collecting pipe (202) of the collecting device (200) is connected with a sampling pipe (301) of the sampling device (300) through the hose (400); the collecting device (200) is a filter membrane type collecting device or a culture medium type collecting device;
the filter membrane type collecting device comprises a conical upper pressing body (203), a filter membrane (204) and a conical lower pressing body (205), wherein an exhaust pipe (201) is connected to the conical upper pressing body (203), a collecting pipe (202) is connected to the conical lower pressing body (205), the filter membrane (204) is arranged between the conical upper pressing body (203) and the conical lower pressing body (205), and the conical upper pressing body (203), the filter membrane (204) and the conical lower pressing body (205) are fixed together through bolts;
the culture medium type collecting device comprises a bottle body (206) and a plurality of supporting frames (207) loaded with solid culture media, wherein two ends of the bottle body (206) are respectively connected with an exhaust pipe (201) and a collecting pipe (202).
2. The device for sampling a biological sample in ancient calligraphy and painting on ancient book file according to claim 1, characterized in that, there is a sealing ring (208) between the cone-shaped upper pressing body (203) and the cone-shaped lower pressing body (205).
3. The sampling device suitable for the biological samples in the ancient calligraphy and painting of ancient book archives according to claim 1, characterized in that, the sampling device (300) is an all-round sampling device, the all-round sampling device comprises a sampling body (303) and a support body (308), the sampling body (303) is provided with a sampling port I (304) and a pin hole (305) and is connected with a sampling pipe (301), the support body (308) is provided with an air inlet (306) and a pin (307) and is connected with an air inlet pipe (302), the sampling body (303) and the support body (308) are fixed together by inserting the pin (307) into the pin hole (305), and the ancient calligraphy and painting sample (500) of ancient book archives to be sampled is arranged between the sampling body (303) and the support body (308).
4. The device for sampling biological samples in ancient scripts and paintings on ancient book file according to claim 3, wherein the outer edges of the sampling port I (304) and the air inlet (306) are smaller than the outer edge of the ancient calligraphy and painting sample (500) of the ancient book file to be sampled.
5. The sampling device for the biological samples in the ancient scripts and paintings of ancient book archives according to claim 1, characterized in that the sampling device (300) is a point sampling device, the point sampling device comprises a main body I (309), the upper end of the main body I (309) is connected with a sampling tube (301), and the lower end is provided with a sampling port II (310).
6. The sampling device for the biological samples in ancient scripts and paintings of ancient book file according to claim 1, characterized in that, said sampling device (300) is a face type sampling device, said face type sampling device comprises a main body II (311), one end of the main body II (311) is connected with the sampling tube (301), and the other end is a sampling port III (312).
7. The sampling device for the biological samples in ancient scripts and paintings in ancient book archives of claim 1, wherein the sampling device (300) is a line type sampling device, the line type sampling device comprises a main body III (313), one end of the main body III (313) is connected with the sampling tube (301), and the other end of the main body III is a sampling port IV (314).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120404622.6U CN215828769U (en) | 2021-02-24 | 2021-02-24 | Sampling device suitable for biological sample in ancient calligraphy and painting of ancient book archives |
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| CN202120404622.6U CN215828769U (en) | 2021-02-24 | 2021-02-24 | Sampling device suitable for biological sample in ancient calligraphy and painting of ancient book archives |
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| CN215828769U true CN215828769U (en) | 2022-02-15 |
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| CN202120404622.6U Active CN215828769U (en) | 2021-02-24 | 2021-02-24 | Sampling device suitable for biological sample in ancient calligraphy and painting of ancient book archives |
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