CN215812825U - Reagent card for multi-index joint inspection of thrombus related diseases - Google Patents
Reagent card for multi-index joint inspection of thrombus related diseases Download PDFInfo
- Publication number
- CN215812825U CN215812825U CN202021854951.2U CN202021854951U CN215812825U CN 215812825 U CN215812825 U CN 215812825U CN 202021854951 U CN202021854951 U CN 202021854951U CN 215812825 U CN215812825 U CN 215812825U
- Authority
- CN
- China
- Prior art keywords
- panel
- bottom plate
- detection
- thrombus
- antibody
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The utility model relates to a reagent card for multi-index joint inspection of diseases related to thrombus, which comprises a card sleeve and test strips fixed in the card sleeve, wherein the card sleeve is divided into a panel and a bottom plate matched with the panel, the panel and the bottom plate are connected through bulges and grooves at the periphery of the panel and the bottom plate in a buckling mode, at least two fixing grooves are formed in the bottom plate, one test strip is fixedly adhered in each fixing groove, a test window and a sample inlet hole are formed in the position, fixed corresponding to each test strip, on the panel, and a handheld area with uniformly distributed convex points is arranged on one side of the panel so as to be convenient to hold. The multi-index joint inspection reagent card has flexible detection index setting, adopts a plurality of groups of detection units, and each detection unit can be provided with an independent sample inlet, thereby being capable of simultaneously detecting multiple indexes in thrombus related diseases and being suitable for single index or single detection of a plurality of indexes; multiple sets of detection units can be identified by color or letters to avoid detection errors caused by negligence.
Description
Technical Field
The utility model relates to a reagent card for multi-index joint inspection of thrombus-related diseases, belonging to the field of detection equipment.
Background
Thrombus is a disease with hidden onset, sudden onset and high lethality disability rate, can be seen in almost all clinical departments, and the pathological process of the thrombus relates to three major systems of vascular endothelium, blood coagulation and fibrinolysis. Studies have shown that the vascular endothelial, coagulation and fibrinolytic systems have been altered when the body is in a prothrombotic state, D-Dimer (DD), Thrombin-antithrombin III complex (TAT), Plasmin- α 2 Plasmin Inhibitor Complex (PIC), thrombomodulin (TM), Fibrin (ogen) degradation products (FDP) and Tissue plasminogen activator-plasminogen activator inhibitor-1 complex (t-PAI.C) are effective indexes reflecting early changes of vascular endothelium, blood coagulation and fibrinolysis systems of organisms, and are suitable for early diagnosis, risk assessment and treatment efficacy assessment of thrombus of high risk groups of thrombus in various clinical subjects and thrombus risk screening of healthy groups.
The traditional detection of thrombus related diseases is detection of single index, and the obtained data is single and incomplete, so that the diagnosis is slow, and the clinical treatment of patients is influenced. Therefore, how to rapidly and accurately detect multiple indexes of the thrombus-related diseases at the same time is a problem to be solved.
SUMMERY OF THE UTILITY MODEL
In order to overcome the problems of the prior art, the utility model provides a reagent card for the multi-index joint inspection of thrombus-related diseases. The multi-index joint inspection reagent card can be used for carrying out quantitative detection on a plurality of indexes in thrombus related diseases at one time, and is also suitable for single-index or single detection of a plurality of indexes.
The purpose of the utility model is realized as follows:
the utility model provides a many indexs of thrombus related disease joint inspection reagent card, includes the cutting ferrule and is fixed in the test paper strip in the cutting ferrule, wherein, the cutting ferrule divide into panel and the supporting bottom plate with it, and both connect through panel and bottom plate arch all around and recess buckle, set up two at least fixed slots in the bottom plate, paste a fixed test paper strip in every fixed slot, correspond every fixed position of test paper strip on the panel and set up detection window and advance the appearance hole, set up the handheld district of evenly arranging the bump in one side of panel and be convenient for control.
Further, the detection test strip is a fluorescence immunochromatography detection test strip.
Furthermore, the detection test strip is formed by sequentially overlapping and adhering a sample pad, a fluorescence labeling pad, a nitrocellulose membrane and absorbent paper to a bottom lining.
Further, the label coated on the fluorescent label pad comprises a DD antibody label, a TM antibody label, a TAT antibody label, a PIC antibody label, an FDP antibody label, and a tpa.
Furthermore, the lower surface of the bottom plate is provided with a concave point for positioning.
Furthermore, one side of the lower surface of the bottom plate is also provided with corresponding clamping grooves corresponding to the fixing grooves.
The utility model has the advantages and beneficial effects that:
(1) the detection index is flexibly set: a plurality of groups of detection units are adopted, and each detection unit can be provided with an independent sample inlet, so that simultaneous detection of multiple indexes (including TAT, PIC, TM and t-PAI-C) in the thrombus related diseases can be realized, and the method is also suitable for single detection of single index or single detection of a plurality of indexes; the multiple groups of detection units can be identified by colors or letters so as to avoid errors caused by detection negligence;
(2) the operation is simple: the processed sample solution is only required to be dripped into a detection sample adding groove (if single index or a plurality of indexes are separately detected, the sample solution is only required to be dripped into corresponding sample feeding holes), and after a certain reaction time, the detection result can be quickly obtained by matching with corresponding detection equipment;
(3) the detection sample feeding holes of the detection units are arranged at equal intervals, so that the consistency of the sample feeding amount and the sample feeding time of the detection units is ensured, the reliability of a detection result is ensured to the maximum extent, the sensitivity is high, and the errors in batches and among batches are small, thereby providing great convenience for clinical use; moreover, the volume is small, the carrying is convenient, the storage is easy, and the application and the popularization of a primary hospital are facilitated; in addition, the preparation method is simple, the technology is mature, and batch production can be realized;
(4) the fixed grooves are mutually spaced, so that the test strips are mutually spaced, the detection items cannot be subjected to cross contamination, and the detection result cannot be deviated due to mutual influence of chromatographic action; simultaneously, one side of the lower surface of bottom plate corresponds every fixed slot and also is equipped with corresponding draw-in groove, can effectively prevent that the reagent card from placing the dislocation when arranging check out test set in.
Drawings
The utility model is further illustrated by the following figures and examples.
FIG. 1 is a schematic structural diagram of a reagent card for multiple-index joint inspection of thrombosis related diseases according to an embodiment of the present invention;
FIG. 2 is a schematic view of a lower surface structure of the panel according to the embodiment of the present invention;
FIG. 3 is a schematic view of the upper surface structure of the base plate according to the embodiment of the present invention;
FIG. 4 is a schematic view of a bottom surface structure of the base plate according to the embodiment of the present invention;
FIG. 5 is a schematic structural diagram of the test strip according to the embodiment of the present invention.
Detailed Description
The first embodiment is as follows:
as shown in fig. 1-4, the present embodiment is a reagent card for joint inspection of six indexes of thrombus related diseases, which includes a card sleeve and test strips fixed in the card sleeve, wherein the card sleeve is divided into a panel 1 and a bottom plate 2 matched therewith, the panel and the bottom plate are connected by a protrusion and a groove buckle around the panel and the bottom plate, six fixing grooves 2-1 are formed in the bottom plate, a test strip 3, which is respectively 6 test strips of DD, TM, TAT, PIC, FDP, and tpa i.c, is fixedly adhered in each fixing groove, a test window 1-1 and a sample inlet 1-2 are formed in the panel at a position corresponding to each test strip, and a hand-held area 1-3 with uniformly arranged bumps is formed in one side of the panel for easy holding and convenient operation. In order to improve the detection efficiency, the reagent card for the six-index joint inspection of the thrombus related diseases is used in cooperation with an analyzer of an immune system, in order to prevent the reagent card from being misplaced when the reagent card is placed in the analyzer, a concave point 2-2 for positioning is arranged on one side of the lower surface of the bottom plate, and a corresponding clamping groove 2-3 is also arranged corresponding to each fixing groove.
As shown in fig. 5, the test strip 3 is a fluorescence immunochromatographic test strip, and is formed by sequentially overlapping and adhering a sample pad 301, a fluorescence label pad 302, a nitrocellulose membrane 303, and a water absorbent paper 304 to a bottom substrate 305. The fluorescent substance labeling pads 302 of the 5 test strips are respectively coated with a DD antibody marker, a TM antibody marker, a TAT antibody marker, a PIC antibody marker, an FDP antibody marker and a tPAI.C antibody marker, and the nitrocellulose membrane 303 is respectively coated with a DD antibody, a TM antibody, a TAT antibody, a PIC antibody, an FDP antibody and a tPAI.C antibody to form a detection area and a goat anti-mouse IgG antibody to form a control area. The detection window 1-1 on the panel corresponds to the detection area of the detection test strip, and the sample inlet 1-2 corresponds to the sample pad 301.
The reagent card described in this embodiment is used for detection at room temperature, and the detection steps are as follows:
when the kit is used, a sample liquid is dripped on a sample pad through a sample inlet hole 1-2, the sample liquid flows towards one side of absorbent paper under the capillary action, when DD, TM, TAT, PIC, FDP and tPAI.C are contained in the sample liquid, DD, TM, TAT, PIC, FDP and tPAI.C respectively form an antigen-antibody complex with a TM antibody, a TAT antibody, a PIC antibody, an FDP antibody and a tPAI.C antibody which are marked by a fluorescent substance, and the antigen-antibody complex continuously flows towards one side of the absorbent paper along with the chromatography action to reach the DD antibody, the TM antibody, the TAT antibody, the PIC antibody, the FDP antibody and the tPAI.C antibody coated in a detection area T to form an antibody-antigen-antibody complex, and an aggregate of the fluorescent substance with the antibody-antigen-antibody complex is formed at the T. And the fluorescent substance which is not combined with the monoclonal antibody continuously swims to one side of the absorbent paper, and when the fluorescent substance reaches the quality control band C, the goat anti-mouse IgG antibody is combined with the mouse-derived monoclonal antibody on the fluorescent substance, so that the fluorescent substance is gathered at the position of the quality control band C.
Finally, it should be noted that the above only illustrates the technical solution of the present invention, but not limited thereto, and although the present invention has been described in detail with reference to the preferred arrangement, it will be understood by those skilled in the art that modifications or equivalent substitutions may be made thereto without departing from the spirit and scope of the technical solution of the present invention.
Claims (2)
1. A reagent card for multi-index joint inspection of thrombus related diseases comprises a card sleeve and test strips fixed in the card sleeve, and is characterized in that the card sleeve is divided into a panel (1) and a bottom plate (2) matched with the panel, the panel and the bottom plate are connected through bulges and grooves at the periphery of the panel and the bottom plate in a buckling mode, at least two fixing grooves (2-1) are formed in the bottom plate, a test strip (3) is fixedly adhered in each fixing groove, a test window (1-1) and a sample inlet hole (1-2) are formed in the panel corresponding to the position where each test strip is fixed, a handheld area (1-3) with uniformly distributed convex points is arranged on one side of the panel to facilitate holding, and corresponding clamping grooves (2-3) are also formed in the lower surface of the bottom plate corresponding to each fixing groove (2-1);
the detection test strip (3) is a fluorescence immunochromatographic detection test strip and is formed by sequentially overlapping and sticking a sample pad (301), a fluorescence labeling pad (302), a nitrocellulose membrane (303) and absorbent paper (304) on a bottom liner (305); the label coated on the fluorescent label pad comprises a DD antibody label, a TM antibody label, a TAT antibody label, a PIC antibody label, an FDP antibody label and a tPAI.C antibody label;
the lower surface of the bottom plate is provided with a concave point (2-2) for positioning;
the reagent card is matched with an immune layer system analyzer for use, and quantitative detection of thrombus related diseases is carried out.
2. The reagent card for the combined detection of multiple indexes of diseases related to thrombus of claim 1, wherein the number of the fixing grooves (2-1) is 6.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202021854951.2U CN215812825U (en) | 2020-08-31 | 2020-08-31 | Reagent card for multi-index joint inspection of thrombus related diseases |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202021854951.2U CN215812825U (en) | 2020-08-31 | 2020-08-31 | Reagent card for multi-index joint inspection of thrombus related diseases |
Publications (1)
Publication Number | Publication Date |
---|---|
CN215812825U true CN215812825U (en) | 2022-02-11 |
Family
ID=80124196
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202021854951.2U Active CN215812825U (en) | 2020-08-31 | 2020-08-31 | Reagent card for multi-index joint inspection of thrombus related diseases |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN215812825U (en) |
-
2020
- 2020-08-31 CN CN202021854951.2U patent/CN215812825U/en active Active
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US6316205B1 (en) | Assay devices and methods of analyte detection | |
CN111033226A (en) | Novel universal test system for quantitative analysis | |
CN101275954B (en) | Breast cancer early warning chip for easily rapid measuring human I type thymidine kinase gene protein | |
CN102662065B (en) | Immunofluorescence dipstick component for quickly and quantitatively detecting protein of plurality of types and detection card component prepared from same and preparation method thereof | |
JPH03501521A (en) | Analytical measurement equipment | |
JP2676108B2 (en) | Immunoenzymatic detection of substances from blood drops or fluids from any biological medium | |
US9903858B2 (en) | Multiplexing with single sample metering event to increase throughput | |
WO1987003910A1 (en) | Cellular method for determining tissue of origin | |
CN101120253A (en) | Immunochromatographic test instrument and semiquantitative method using the same | |
EP0587222A2 (en) | Dry immunoassay elements with a separate absorbent layer | |
US5935846A (en) | Device for determining the activity of enzymes in liquids | |
CN102135535B (en) | Immune colloidal metal detection technology capable of directly performing semi-quantitative analysis, preparation method and application | |
CN108535485A (en) | A kind of time-resolved fluoroimmunoassay chromatograph test strip and preparation method quantitatively detecting CA153 in blood | |
CN102135498A (en) | Semi-quantitative colloidal metal detection technology taking multi-capture property as characteristic and preparation method and use thereof | |
CN116106539A (en) | Multiple antigen coating kit for immune micro-fluidic control, method and application thereof | |
CN215812825U (en) | Reagent card for multi-index joint inspection of thrombus related diseases | |
US9410952B2 (en) | Method and device for the determination of several analytes with simultaneous internal verification in a graphical combination | |
CN210742281U (en) | Thrombus analyzer and thrombus analyzer system | |
CN209264735U (en) | A kind of high pass amount detecting device for two-step method immunochromatography | |
CN115963256A (en) | Carcinoembryonic antigen fluorescence immunochromatography detection kit and preparation method thereof | |
CN215812816U (en) | Multi-index joint inspection reagent card for respiratory tract infection related diseases | |
WO2014168580A1 (en) | Double-chamber bi-directional reverse flow device | |
CN114167052A (en) | Kit for quantitative detection of NT-proBNP/ST2 by time-resolved fluorescence immunochromatography and application thereof | |
CN210954058U (en) | Magnetic bead releasing mechanism and system | |
CN2672656Y (en) | Diagnostic reagent srip |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
GR01 | Patent grant | ||
GR01 | Patent grant |