CN215775139U

CN215775139U - Cell freezing bag suitable for cell reinfusion

Info

Publication number: CN215775139U
Application number: CN202121304166.4U
Authority: CN
Inventors: 廖静; 戴玲华
Original assignee: Hangzhou S-Evans Biosciences Ltd
Current assignee: Hangzhou S-Evans Biosciences Ltd
Priority date: 2021-06-10
Filing date: 2021-06-10
Publication date: 2022-02-11
Anticipated expiration: 2031-06-10

Abstract

The utility model discloses a cell cryopreservation bag suitable for cell feedback, and relates to the field of cell cryopreservation bags. Compared with the prior art, the utility model has the beneficial effects that: cell in the liquid storage bag can not be polluted when carrying out the cell sample, and box body I and box body II deposit the bag to liquid and have the guard action, prevent that liquid from depositing the bag by the fish tail damaged.

Description

Cell freezing bag suitable for cell reinfusion

Technical Field

The utility model relates to the field of cell cryopreservation bags, in particular to a cell cryopreservation bag suitable for cell reinfusion.

Background

With the development of biological technologies, cell therapy is rapidly developing and increasingly accepted worldwide. The basis of cell therapy is the harvesting and storage of viable cells. In addition, in vitro culture of cells has also been widely used in various fields of biological research. In the in vitro cell culture work, in order to preserve cells and enable the cells to have biological activity for a long time, the freezing and recovery of the cells become a crucial link. The preparation and storage of cells, especially the low-temperature storage and recovery conditions of biological samples such as umbilical cord blood, stem cells and the like and the prevention of pollution of operation processes are important subjects of the current clinical application of cell therapy. Various cell banks are established in many countries and regions, the optimization of cell cryopreservation recovery procedures and the prevention of pollution become the prerequisites for cell production and cell quality guarantee, and stem cells are a type of pluripotent cells with self-replication capacity and can be differentiated into various functional cells under certain conditions. With the continuous and deep research of stem cells, the clinical application range of the stem cells is continuously expanded, and hematopoietic stem cells are the earliest stem cells in clinical application, are the only sources of various blood cells in vivo and mainly exist in bone marrow, peripheral blood and umbilical cord blood. Transplantation of hematopoietic stem cells is an effective method for treating hematological diseases, immune diseases, congenital genetic diseases, and multiple and metastatic malignant diseases, and in the process of stem cell transplantation, cells need to be preserved in the same container from cryopreservation to final transfusion to avoid contamination, cryopreservation is needed after treatment, the biological sample may need to be tested before or after cryopreservation, or used for other purposes, or all biological samples need to be used for treatment purposes if the amount of the biological samples is insufficient.

Among the prior art, make the air in the cell easily when carrying out sample test to the cell, lead to the cell to receive the pollution of bacterium easily, and the cryopreserved bag does not receive the protection, and easy fish tail is damaged, so a section can protect the cell in the cryopreserved bag when carrying out sample test, avoids the cell to receive bacterial contamination and can wait to develop to the cell cryopreserved bag that the suitable cell feedback that the cryopreserved bag body protected remains.

SUMMERY OF THE UTILITY MODEL

In order to solve the defects in the prior art, the utility model provides a cell cryopreservation bag suitable for cell reinfusion, so as to solve the problems that the cells are easily polluted by bacteria because air is easily introduced into the cells when the cells are subjected to sample detection, and the cryopreservation bag is not protected and is easily scratched and damaged.

In order to achieve the purpose, the utility model is realized by the following technical scheme: cell cryopreserving bag that suitable cell feedback used, deposit the bag including liquid, the trilateral of bag is deposited to liquid sets up the banding around setting up, liquid is deposited the bag and is not set up the sample bag on one side of banding, set up aperture and sample bag intercommunication on the liquid is deposited the bag, set up in the sample bag and be used for plugging up the shutoff mechanism of aperture, articulated box body I and box body II on the banding, box body I and box body II are through buckle locking, box body I and II locking back liquid of box body are deposited bag and sample bag and are arranged in inside box body I and box body II, and one of them banding sets up the feed liquor pipe and the drain pipe of bag intercommunication with liquid, the one end of feed liquor pipe sets up the butterfly clamp connector, set up regulator and tighrening ring on the drain pipe, the tighrening ring is located between banding and the regulator.

The plugging mechanism comprises a spring and a piston, the end part of the piston is connected with the small hole in the liquid storage bag in an inserting mode, the spring is arranged outside the piston, and the two ends of the spring are respectively fixedly connected with one end of the piston and the inside of the sample bag.

And a round hole for hanging the cryopreservation bag is formed in the sealing edge opposite to the liquid outlet pipe.

The round hole inner ring is provided with a protective ring for preventing the round hole from being broken.

A label groove with one open end is formed in the box body I.

The label slot is characterized in that one end of the opening of the label slot is connected with a label cover, and the label cover is connected with the label slot through a snap fastener.

The sample bag is provided with a sampling tube, and the end part of the sampling tube is provided with a sealing cover.

Compared with the prior art, the utility model has the beneficial effects that:

1. offer aperture and sample bag intercommunication on the liquid is deposited the bag, set up in the sample bag and be used for plugging up the shutoff mechanism of aperture, can avoid the cell contact air in the liquid is deposited the bag when taking a sample, avoid the contaminated risk of cell.

2. Articulated box body I and box body II on the banding, box body I and box body II pass through buckle locking, and box body I and II locking back liquid of box body deposit the bag and sample bag arrange box body I and box body II insidely in, can deposit the bag to liquid and protect, avoid unexpected fish tail liquid to deposit the bag and lead to liquid to deposit the bag damage, cause the loss.

Drawings

FIG. 1 is a schematic diagram of the overall structure of the present invention;

FIG. 2 is a schematic view of a fluid storage bag according to the present invention;

FIG. 3 is a schematic structural diagram of the plugging mechanism of the present invention.

Reference numerals shown in the drawings: 1. a liquid storage bag; 2. sealing edges; 3. a sample bag; 4. a box body I; 5. a box body II; 6. a liquid inlet pipe; 7. a liquid outlet pipe; 8. a regulator; 9. a fastening ring; 10. a spring; 11. a piston; 12. a circular hole; 13. a label slot; 14. a label cover; 15. a sampling tube; 16. a sealing cover; 17. snapping; 18. buckling; 19. butterfly clip connecting port.

Detailed Description

The utility model will be further illustrated with reference to the following specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Further, it should be understood that various changes or modifications of the present invention may be made by those skilled in the art after reading the teaching of the present invention, and these equivalents also fall within the scope of the present application.

As shown in fig. 1-3, a cell cryopreservation bag suitable for cell feedback comprises a liquid storage bag 1, wherein three edges of the liquid storage bag 1 are surrounded by a sealing edge 2, a sample bag 3 is arranged on one side of the liquid storage bag 1 without the sealing edge 2, a small hole is formed in the liquid storage bag 1 and communicated with the sample bag 3, a plugging mechanism for plugging the small hole is arranged in the sample bag 3, the plugging mechanism prevents air from entering the liquid storage bag 1 during sample sampling to cause cell contamination in the liquid storage bag 1, a box body i 4 and a box body ii 5 are hinged on the sealing edge 2, the box body i 4 and the box body ii 5 are locked through a buckle 18, the liquid storage bag 1 and the sample bag 3 are arranged in the box body i 4 and the box body ii 5 after the box body i 4 and the box body ii 5 are locked, and when in use, the liquid storage bag 1 can be protected after the box body i 4 and the box body ii 5 are fastened through the buckle 18, the liquid storage bag 1 is prevented from being damaged due to scratching, and medical resources are prevented from being wasted. Set up on one of them banding 2 and deposit feed liquor pipe 6 and drain pipe 7 of bag 1 intercommunication with liquid, the one end of feed liquor pipe 6 sets up butterfly clamp connector 19, set up regulator 8 and tighrening ring 9 on the drain pipe 7, tighrening ring 9 is located between banding 2 and the regulator 8, and feed liquor pipe 6 is collected the cell and is deposited bag 1 and sealed with butterfly clamp connector 19 in and, makes liquid deposit bag 1 leakproofness better, and the cell can carry out the cell feedback through drain pipe 7 when carrying out the cell feedback, can adjust the speed of cell feedback through regulator 8.

As shown in fig. 3, the plugging mechanism includes a spring 10 and a piston 11, an end of the piston 11 is inserted into and connected with a small hole on the liquid storage bag 1, the spring 10 is disposed outside the piston 11, two ends of the spring 10 are respectively fixedly connected with one end of the piston 11 and the inside of the sample bag 3, a sampling tube 15 is disposed on the sample bag 3, and a sealing cover 16 is disposed at an end of the sampling tube 15.

When carrying out sample detection to the cell in the liquid storage bag 1, open sealed lid 16, extrude sample bag 3, the air in the sample bag 3 is discharged through sampling tube 15, use the cylinder to take a sample through sampling tube 15, because the pressure that the cylinder extracted makes piston 11 to the inside motion of sample bag 3, the cell in the liquid storage bag 1 gets into the cylinder this moment, stop the pull cylinder after the sample is accomplished, piston 11 blocks up the aperture on the liquid storage bag 1 through the effort of spring 10, avoid the air to get into the liquid storage bag 1 and lead to the cell in the liquid storage bag 1 to be contaminated by the bacterium.

Further, set up the round hole 12 that is used for hanging the cryopreserved bag on the banding 2 relative with drain pipe 7, round hole 12 inner circle sets up prevents the cracked guard circle of round hole 12, and round hole 12 can hang the cryopreserved bag when carrying out cell feedback, facilitates the use.

Further, set up one end open-ended label groove 13 on box body I4, can place label such as the name of the cell of depositing in the bag 1, date in the label groove 13 about liquid, make the cell of depositing in the bag 1 be distinguished more easily, improve staff's work efficiency.

Further, one end of the opening of the label groove 13 is connected with a label cover 14, and the label cover 14 is connected with the label groove 13 through a snap 17, so that labels are prevented from falling off in the transportation process.

Claims

1. Cell cryopreserving bag that suitable cell reinfusion used, including liquid storage bag (1), its characterized in that: the trilateral of bag (1) is deposited to liquid sets up banding (2) around setting up, liquid is deposited bag (1) and is not set up sample bag (3) on one side of banding (2), set up aperture and sample bag (3) intercommunication on liquid is deposited bag (1), set up in sample bag (3) and be used for plugging up the shutoff mechanism of aperture, articulated box body I (4) and box body II (5) are gone up in banding (2), box body I (4) and box body II (5) are locked through buckle (18), box body I (4) and box body II (5) are locked the back liquid and are deposited bag (1) and sample bag (3) and arrange box body I (4) and box body II (5) inside in, set up feed liquor pipe (6) and drain pipe (7) that bag (1) communicate with liquid on one of them banding (2), the one end of feed liquor pipe (6) sets up and presss from both sides connector (19), set up regulator (8) and tighrening ring (9) on drain pipe (7), tighrening ring (9) are located between banding (2) and regulator (8).

2. The cell cryopreservation bag suitable for cell transfusion as claimed in claim 1, wherein: the plugging mechanism comprises a spring (10) and a piston (11), the end of the piston (11) is connected with the small hole in the liquid storage bag (1) in a penetrating mode, the spring (10) is arranged outside the piston (11), and the two ends of the spring (10) are fixedly connected with one end of the piston (11) and the inside of the sample bag (3) respectively.

3. The cell cryopreservation bag suitable for cell transfusion as claimed in claim 1, wherein: and a round hole (12) for hanging the freezing storage bag is arranged on the sealing edge (2) opposite to the liquid outlet pipe (7).

4. The cell cryopreservation bag suitable for cell transfusion as claimed in claim 3, wherein: the inner ring of the round hole (12) is provided with a protective ring for preventing the round hole (12) from being broken.

5. The cell cryopreservation bag suitable for cell transfusion as claimed in claim 1, wherein: a label groove (13) with one open end is formed in the box body I (4).

6. The cell cryopreservation bag suitable for cell transfusion as claimed in claim 5, wherein: one end of the opening of the label groove (13) is connected with a label cover (14), and the label cover (14) is connected with the label groove (13) through a snap fastener (17).

7. The cell cryopreservation bag suitable for cell transfusion as claimed in claim 1, wherein: the sampling device is characterized in that a sampling tube (15) is arranged on the sample bag (3), and a sealing cover (16) is arranged at the end part of the sampling tube (15).