CN215768606U

CN215768606U - Immunohistochemical wet box convenient to slide glass is washd

Info

Publication number: CN215768606U
Application number: CN202122190811.0U
Authority: CN
Inventors: 张艳阁; 赵付荣; 熊维彬; 吴铜鹏
Original assignee: Minjiang University
Current assignee: Minjiang University
Priority date: 2021-09-10
Filing date: 2021-09-10
Publication date: 2022-02-08
Anticipated expiration: 2031-09-10

Abstract

The utility model belongs to the technical field of biomedical experimental equipment, and relates to an immunohistochemical wet box convenient for glass slide cleaning, which comprises: a box body with an opening at the upper part; the cover body is arranged at the upper opening of the box body and is buckled with the box body; the base is arranged at the bottom in the box body, and the upper surface of the base is uniformly provided with a plurality of placing grooves for placing glass slides; the liquid outlet is arranged at the bottom of the front wall of the box body; the angle adjusting mechanism is arranged inside the box body and positioned at one end of the lower part of the base and used for adjusting the inclination angle of the base. According to the utility model, through the arrangement of the angle adjusting mechanism, the glass slides can be cleaned in the wet box in the whole immunohistochemical process, the glass slides do not need to be taken out and cleaned one by one, the time required by cleaning is reduced, the working efficiency is improved, meanwhile, the flow rate of the cleaning solution can be adjusted by accurately adjusting the inclination angle, the flow rate of the cleaning solution is adjusted according to different cleaning difficulties, and the cleaning speed can be improved to the maximum extent while the cleanness of the glass slides is ensured.

Description

Immunohistochemical wet box convenient to slide glass is washd

Technical Field

The utility model belongs to the technical field of biomedical experimental equipment, and relates to an immunohistochemical wet box convenient for cleaning a glass slide.

Background

Immunohistochemistry is the research of determining the antigens in tissue cells by using the basic principle of immunology, namely antigen-antibody reaction, namely the principle of specific combination of antigens and antibodies, and developing color developing agents for marking antibodies through chemical reaction, and carrying out positioning, qualitative and relative quantification on the antigens, and is called immunohistochemistry and also called immunocytochemistry. It is a branch of histochemistry, and all substances with antigenicity in tissue cells, such as peptides, hormones, neurotransmitters, cytokines, receptors, surface antigens, and the like, can be displayed by an immunohistochemical method, so that the method is widely applied to basic and clinical scientific researches at present. However, after the existing immunohistochemical wet box is incubated, the slide glass needs to be taken out for cleaning, and then the slide glass is put back into the wet box, so that another reagent can be added, the time required by the experiment is prolonged, and the working efficiency is reduced.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide an immunohistochemical wet box convenient for cleaning a glass slide, and aims to solve the technical problem that the work efficiency is influenced because the conventional dampness removing box cannot clean the glass slide.

In order to realize the purpose, the specific technical scheme of the immunohistochemical wet box convenient for cleaning the glass slide is as follows:

an immunohistochemical wet cartridge for facilitating slide cleaning, comprising:

a box body with an opening at the upper part;

the cover body is arranged at the upper opening of the box body and is buckled with the box body;

the base is arranged at the bottom of the front wall in the box body, and the upper surface of the base is uniformly provided with a plurality of placing grooves for placing glass slides;

the liquid outlet is arranged at the bottom of the box body;

the angle adjusting mechanism is arranged inside the box body and positioned at one end of the lower part of the base and used for adjusting the inclination angle of the base.

The present invention is also characterized in that,

the angle adjusting mechanism comprises a transmission rod, the transmission rod is transversely arranged at the bottom in the box body, one end of the transmission rod is connected onto one side wall of the box body through a bearing, the other end of the transmission rod penetrates through the other side wall, opposite to the box body, of the box body and then is connected with a knob, a first bevel gear is sleeved on the transmission rod, a lead screw is vertically arranged at the position, close to the transmission rod, of the lower portion of the base, a limiting barrel is vertically arranged at the position, close to the transmission rod, of the bottom of the box body, one end of the lead screw is hinged to the lower portion of the base, the other end of the lead screw is inserted into the limiting barrel, a second bevel gear is sleeved on the lead screw and is in threaded connection with the second bevel gear, the second bevel gear is in meshed connection with the first bevel gear, the side face, connected with a rotating shaft, of the transmission rod, and two opposite inner walls of the box body are connected with the two ends of the rotating shaft through bearings.

Wherein, a circle of scale marks are arranged on the outer wall of the box body around the knob.

Wherein a plurality of standing grooves become array distribution, and one side that each standing groove is close to the pivot is provided with first drainage groove, and two adjacent standing grooves are linked together in the first drainage groove messenger longitudinal direction, and first drainage groove messenger is close to the standing groove and the base outside of pivot and is linked together.

The side surface of the rotating shaft, which is far away from the base, is adjacent to the side wall of the box body, the positions, close to each first liquid discharge groove, of the side wall of the box body are respectively and vertically provided with a plurality of second liquid discharge grooves, the plurality of liquid discharge ports are respectively arranged at the positions, close to the second liquid discharge grooves, and the lower portion of each second liquid discharge groove is communicated with the liquid discharge ports.

Each liquid outlet is connected with a hose, and the hose is hung on the front wall of the outer part of the box body through a hook.

Wherein, a plurality of bulges are uniformly arranged at the bottom of each placing groove and used for supporting the glass slide.

The immunohistochemical wet box convenient for cleaning the glass slide has the following advantages: through angle adjustment mechanism's setting, realized at whole immunohistochemical in-process, can make the slide wash in wet box, need not to take out the slide one by one again and wash, reduced and washd the required time, improved work efficiency, simultaneously, can adjust the lotion velocity of flow with the adjustment cleaning solution velocity of flow through accurate adjustment inclination, to wasing the degree of difficulty difference, can improve the cleaning speed in the at utmost when guaranteeing the sanitization.

Drawings

FIG. 1 is a schematic diagram of the overall configuration of an immunohistochemical wet cassette for facilitating slide cleaning according to the present invention;

FIG. 2 is a schematic diagram of an external front view of an immunohistochemical wet cassette for facilitating slide cleaning according to the present invention;

FIG. 3 is a schematic diagram of an internal top view of an immunohistochemical wet cassette for facilitating slide cleaning according to the present invention;

reference numerals:

1. a box body; 2. a cover body; 3. a base; 4. a placement groove; 5. a liquid discharge port; 6. a transmission rod; 7. a knob; 8. a first bevel gear; 9. a second bevel gear; 10. a screw rod; 11. a limiting cylinder; 12. a rotating shaft; 13. a first drain tank; 14. a second drain tank; 15. and (4) protruding.

Detailed Description

The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.

As shown in figures 1 and 2, the immunohistochemical wet box convenient for cleaning glass slides comprises a box body 1, the upper part of the box body 1 is provided with an opening, the upper part of the box body 1 is provided with a cover body 2, the cover body 2 and the box body 1 are mutually buckled, the bottom in the box body 1 is provided with a base 3, the upper surface of the base 3 is uniformly provided with 9 placing grooves 4, the 9 placing grooves 4 are arranged in 3 rows and 3 columns for placing the glass slides, the bottom in each placing groove 4 is uniformly provided with 4 bulges 15 for supporting the glass slides and avoiding the glass slides from being polluted by residual liquid at the bottoms of the placing grooves 4, the bottom of the front wall of the box body 1 is provided with a liquid discharge port 5 for discharging cleaning liquid for cleaning the glass slides out of the box body 1, the liquid discharge port 5 is connected with a hose, the hose is hung on the front wall outside the box body 1 through a hook, the hose can be taken down from the box body 1 during liquid discharge, an angle adjusting mechanism is arranged at one end of the box body 1, which is positioned at the lower part of the base 3, and is used for adjusting the inclination angle of the base 3 when the glass slide is cleaned, so that the cleaning liquid flows out from the placing groove 4.

The angle adjusting mechanism comprises a transmission rod 6, the transmission rod 6 is transversely arranged at the bottom in the box body 1, one end of the transmission rod 6 is connected to one side wall of the box body 1 through a bearing, the other end of the transmission rod 6 penetrates through the other opposite side wall of the box body 1 and then is connected with a knob 7, a first bevel gear 8 is sleeved on the transmission rod 6, a lead screw 10 is vertically arranged at the position, close to the transmission rod 6, of the lower part of the base 3, a limiting cylinder 11 is vertically arranged at the position, close to the transmission rod 6, of the bottom of the box body 1, one end of the lead screw 10 is hinged with the lower part of the base 3, the other end of the lead screw 10 is inserted into the limiting cylinder 11, the lead screw 10 can freely move up and down in the limiting cylinder 11, a second bevel gear 9 is sleeved on the lead screw 10, the lead screw 10 is in threaded connection with the second bevel gear 9, the second bevel gear 9 is in meshed connection with the first bevel gear 8, and the side surface, far away from the transmission rod 6, which is connected with a rotating shaft 12, on the base 3, the two ends of the rotating shaft 12 are connected to the two opposite inner walls of the box body 1 through bearings, and the base 3 can rotate around the rotating shaft 12.

A circle of scale marks are arranged on the outer wall of the box body 1 around the knob 7, and each scale mark corresponds to the ascending distance of the free end of the base 3, so that the inclined angle of the base 3 is accurately adjusted, the downward flow speed of the cleaning liquid is controlled, and the cleaning speed is controlled.

As shown in FIG. 3, a plurality of placing grooves 4 are arranged in an array, a first liquid discharge groove 13 is arranged on one side of each placing groove 13 close to the rotating shaft 12, the first liquid discharge groove 13 communicates two placing grooves 4 adjacent to each other in the longitudinal direction, and the first liquid discharge groove 13 communicates the placing grooves 4 close to the rotating shaft 12 with the outside of the base 3, so that the cleaning liquid can rapidly flow from one placing groove 4 to the next placing groove 4 until flowing out of the base 3, and the cleaning liquid can rapidly flow through each slide, thereby improving the cleaning speed.

The degree of depth of standing groove 4 is not less than the degree of depth of first drainage groove 13, guarantees when not flowing back, and standing groove 4 bottom can add water, guarantees the humidity of slide glass, and protruding 15 height is not less than standing groove 4 and the difference in depth of first drainage groove 13, and the page height that just that is protruding 15 height is greater than standing groove 4 bottom watered, guarantees that the water of bottom can not be touched to the slide glass.

The side of the rotating shaft 12 far away from the base 3 is adjacent to the side wall of the box body 1, the positions of the side wall of the box body 1, which are close to each first liquid discharge groove 13, are respectively and vertically provided with a second liquid discharge groove 14, a plurality of liquid discharge ports 5 are respectively arranged at the positions which are close to the second liquid discharge grooves 14, and the lower part of each second liquid discharge groove 14 is communicated with the liquid discharge ports 5. The cleaning liquid flowing out of first liquid discharge tank 13 can be guided by second liquid discharge tank 14 and rapidly flow out of liquid discharge port 5.

Two angles of the bottom of each placing groove 4, which are close to the rotating shaft 12, are arc-shaped, so that the cleaning liquid can flow out of the first liquid discharging groove 13 quickly, and excessive cleaning liquid is prevented from remaining in the placing grooves 4.

The working principle is as follows: after incubation is completed, the cover body 2 is opened, the knob 7 is rotated, the knob 7 drives the transmission rod 6 to rotate, the transmission rod 6 drives the first bevel gear 8 to rotate, the first bevel gear 8 drives the second bevel gear 9 to rotate, the second bevel gear 9 drives the screw rod 10 to move upwards, the screw rod 10 pushes one end of the base 3 to move upwards until the base 3 reaches a proper inclination angle, the hose is taken down from the box body 1, then cleaning liquid is added into the placing groove 4, the cleaning liquid flows to the next placing groove 4 from the first liquid discharge groove 13 after cleaning the glass slides, the glass slides in the placing groove are washed until the glass slides flow out from the last first liquid discharge groove 13, the flow is guided through the second liquid discharge groove 14, then the glass slides are quickly discharged from the hose through the liquid discharge port 5, after cleaning is completed, the knob 7 is rotated, the base 3 is enabled to return to a horizontal position, and then next experiment is carried out.

According to the immunohistochemical wet box convenient for cleaning the glass slide, the glass slide can be cleaned in the wet box in the whole immunohistochemical process through the arrangement of the angle adjusting mechanism, the glass slide does not need to be taken out and cleaned one by one, the time required for cleaning is shortened, the working efficiency is improved, meanwhile, the flow rate of cleaning solution can be adjusted by accurately adjusting the inclination angle, the flow rate of cleaning solution is adjusted according to different cleaning difficulties, and the cleaning speed can be improved to the maximum extent while the cleaning is ensured.

It is to be understood that the present invention has been described with reference to certain embodiments, and that various changes in the features and embodiments, or equivalent substitutions may be made therein by those skilled in the art without departing from the spirit and scope of the utility model. In addition, many modifications may be made to adapt a particular situation or material to the teachings of the utility model without departing from the essential scope thereof. Therefore, it is intended that the utility model not be limited to the particular embodiment disclosed, but that the utility model will include all embodiments falling within the scope of the appended claims.

Claims

1. An immunohistochemical wet cartridge for facilitating slide cleaning, comprising:

a box body (1) with an opening at the upper part;

the cover body (2) is arranged at the upper opening of the box body (1) and is buckled with the box body (1);

the base (3) is arranged at the bottom in the box body (1), and the upper surface of the base is uniformly provided with a plurality of placing grooves (4) for placing glass slides;

the liquid outlet (5) is arranged at the bottom of the front wall of the box body (1);

the angle adjusting mechanism is arranged inside the box body (1), is positioned at one end of the lower part of the base (3) and is used for adjusting the inclination angle of the base (3).

2. The immunohistochemical wet box convenient for glass slide cleaning according to claim 1, wherein the angle adjusting mechanism comprises a transmission rod (6), the transmission rod (6) is transversely arranged at the bottom in the box body (1), one end of the transmission rod (6) is connected to one side wall of the box body (1) through a bearing, the other end of the transmission rod (6) passes through the other opposite side wall of the box body (1) and then is connected with a knob (7), a first bevel gear (8) is sleeved on the transmission rod (6), a lead screw (10) is vertically arranged at the position of the lower part of the base (3) close to the transmission rod (6), a limiting cylinder (11) is vertically arranged at the position of the bottom of the box body (1) close to the transmission rod (6), one end of the lead screw (10) is hinged with the lower part of the base (3), and the other end of the lead screw (10) is inserted into the limiting cylinder (11), the lead screw (10) is sleeved with a second bevel gear (9), the lead screw (10) is in threaded connection with the second bevel gear (9), the second bevel gear (9) is in meshed connection with the first bevel gear (8), the side face, far away from the transmission rod (6), of the base (3) and connected with a rotating shaft (12), and two ends of the rotating shaft (12) are connected to two opposite inner walls of the box body (1) through bearings.

3. An immunohistochemical wet kit for facilitating slide cleaning according to claim 2, wherein a circle of graduation marks is provided on the outer wall of the kit body (1) around the knob (7).

4. An immunohistochemical wet kit for facilitating slide glass washing according to claim 1, wherein a plurality of said placing grooves (4) are arranged in an array, each of said placing grooves (4) is provided with a first drainage groove (13) near one side of the rotating shaft (12), said first drainage groove (13) connects two placing grooves (4) adjacent in the longitudinal direction, said first drainage groove (13) connects the placing groove (4) near the rotating shaft (12) with the outside of the base (3).

5. The immunohistochemical wet box convenient for slide glass cleaning according to claim 4, wherein the side of the rotating shaft (12) far from the base (3) is adjacent to the side wall of the box body (1), a plurality of second liquid discharge grooves (14) are vertically arranged on the side wall of the box body (1) near to each first liquid discharge groove (13), a plurality of liquid discharge ports (5) are respectively arranged on the side wall of the box body near to each second liquid discharge groove (14), and the lower part of each second liquid discharge groove (14) is communicated with the liquid discharge port (5).

6. An immunohistochemical wet box for facilitating slide glass cleaning according to claim 5, wherein each of said drain ports (5) is connected with a flexible tube, and said flexible tube is hung on the outer front wall of the box body (1) through a hook.

7. An immunohistochemical wet cartridge convenient for slide cleaning according to claim 1, wherein each of said placing grooves has a plurality of protrusions (15) uniformly arranged on the bottom for holding the slide.