CN109590170A - Automatic glue pouring production line for preparing electrophoresis gel column, glue pouring method and application - Google Patents
Automatic glue pouring production line for preparing electrophoresis gel column, glue pouring method and application Download PDFInfo
- Publication number
- CN109590170A CN109590170A CN201910093867.9A CN201910093867A CN109590170A CN 109590170 A CN109590170 A CN 109590170A CN 201910093867 A CN201910093867 A CN 201910093867A CN 109590170 A CN109590170 A CN 109590170A
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- Prior art keywords
- glue
- encapsulating
- transmission device
- production line
- gel column
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Links
- 239000003292 glue Substances 0.000 title claims abstract description 227
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 63
- 238000001962 electrophoresis Methods 0.000 title claims abstract description 53
- 238000000034 method Methods 0.000 title claims abstract description 53
- 239000011521 glass Substances 0.000 claims abstract description 34
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 84
- 239000007788 liquid Substances 0.000 claims description 72
- 238000000465 moulding Methods 0.000 claims description 53
- 230000005540 biological transmission Effects 0.000 claims description 37
- 230000001112 coagulating effect Effects 0.000 claims description 33
- 238000002347 injection Methods 0.000 claims description 28
- 239000007924 injection Substances 0.000 claims description 28
- 230000002572 peristaltic effect Effects 0.000 claims description 28
- 239000011232 storage material Substances 0.000 claims description 28
- 239000000853 adhesive Substances 0.000 claims description 26
- 230000001070 adhesive effect Effects 0.000 claims description 26
- 239000002699 waste material Substances 0.000 claims description 23
- 108090001030 Lipoproteins Proteins 0.000 claims description 17
- 102000004895 Lipoproteins Human genes 0.000 claims description 17
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims description 11
- 238000004140 cleaning Methods 0.000 claims description 8
- 239000000084 colloidal system Substances 0.000 claims description 8
- 239000007787 solid Substances 0.000 claims description 8
- 238000010438 heat treatment Methods 0.000 claims description 5
- 239000002351 wastewater Substances 0.000 claims description 5
- 239000000203 mixture Substances 0.000 abstract description 2
- 239000011148 porous material Substances 0.000 abstract 1
- 239000000499 gel Substances 0.000 description 67
- 239000000523 sample Substances 0.000 description 17
- 238000012360 testing method Methods 0.000 description 16
- 239000000243 solution Substances 0.000 description 14
- 239000000047 product Substances 0.000 description 13
- 239000003054 catalyst Substances 0.000 description 8
- 239000003795 chemical substances by application Substances 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 230000033001 locomotion Effects 0.000 description 6
- 238000003860 storage Methods 0.000 description 5
- 108010062497 VLDL Lipoproteins Proteins 0.000 description 4
- 210000002381 plasma Anatomy 0.000 description 4
- 238000012797 qualification Methods 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 108010010234 HDL Lipoproteins Proteins 0.000 description 3
- 102000015779 HDL Lipoproteins Human genes 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 239000011544 gradient gel Substances 0.000 description 3
- 230000005484 gravity Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000007711 solidification Methods 0.000 description 3
- 230000008023 solidification Effects 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 150000003626 triacylglycerols Chemical class 0.000 description 3
- 108010028554 LDL Cholesterol Proteins 0.000 description 2
- 108010007622 LDL Lipoproteins Proteins 0.000 description 2
- 102000007330 LDL Lipoproteins Human genes 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- 108010025899 gelatin film Proteins 0.000 description 2
- 238000011017 operating method Methods 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 238000004886 process control Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000003252 repetitive effect Effects 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 230000003068 static effect Effects 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- 108010010803 Gelatin Proteins 0.000 description 1
- 239000006096 absorbing agent Substances 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 230000003670 easy-to-clean Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 230000003694 hair properties Effects 0.000 description 1
- 238000005213 imbibition Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
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- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
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- 239000007921 spray Substances 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05C—APPARATUS FOR APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05C5/00—Apparatus in which liquid or other fluent material is projected, poured or allowed to flow on to the surface of the work
- B05C5/02—Apparatus in which liquid or other fluent material is projected, poured or allowed to flow on to the surface of the work the liquid or other fluent material being discharged through an outlet orifice by pressure, e.g. from an outlet device in contact or almost in contact, with the work
- B05C5/0208—Apparatus in which liquid or other fluent material is projected, poured or allowed to flow on to the surface of the work the liquid or other fluent material being discharged through an outlet orifice by pressure, e.g. from an outlet device in contact or almost in contact, with the work for applying liquid or other fluent material to separate articles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
- B05B15/00—Details of spraying plant or spraying apparatus not otherwise provided for; Accessories
- B05B15/50—Arrangements for cleaning; Arrangements for preventing deposits, drying-out or blockage; Arrangements for detecting improper discharge caused by the presence of foreign matter
- B05B15/55—Arrangements for cleaning; Arrangements for preventing deposits, drying-out or blockage; Arrangements for detecting improper discharge caused by the presence of foreign matter using cleaning fluids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05C—APPARATUS FOR APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05C11/00—Component parts, details or accessories not specifically provided for in groups B05C1/00 - B05C9/00
- B05C11/02—Apparatus for spreading or distributing liquids or other fluent materials already applied to a surface ; Controlling means therefor; Control of the thickness of a coating by spreading or distributing liquids or other fluent materials already applied to the coated surface
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05C—APPARATUS FOR APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05C11/00—Component parts, details or accessories not specifically provided for in groups B05C1/00 - B05C9/00
- B05C11/10—Storage, supply or control of liquid or other fluent material; Recovery of excess liquid or other fluent material
- B05C11/1002—Means for controlling supply, i.e. flow or pressure, of liquid or other fluent material to the applying apparatus, e.g. valves
- B05C11/1007—Means for controlling supply, i.e. flow or pressure, of liquid or other fluent material to the applying apparatus, e.g. valves responsive to condition of liquid or other fluent material
- B05C11/101—Means for controlling supply, i.e. flow or pressure, of liquid or other fluent material to the applying apparatus, e.g. valves responsive to condition of liquid or other fluent material responsive to weight of a container for liquid or other fluent material; responsive to level of liquid or other fluent material in a container
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05C—APPARATUS FOR APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05C11/00—Component parts, details or accessories not specifically provided for in groups B05C1/00 - B05C9/00
- B05C11/10—Storage, supply or control of liquid or other fluent material; Recovery of excess liquid or other fluent material
- B05C11/1036—Means for supplying a selected one of a plurality of liquids or other fluent materials, or several in selected proportions, to the applying apparatus
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05C—APPARATUS FOR APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05C11/00—Component parts, details or accessories not specifically provided for in groups B05C1/00 - B05C9/00
- B05C11/10—Storage, supply or control of liquid or other fluent material; Recovery of excess liquid or other fluent material
- B05C11/1039—Recovery of excess liquid or other fluent material; Controlling means therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05C—APPARATUS FOR APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05C11/00—Component parts, details or accessories not specifically provided for in groups B05C1/00 - B05C9/00
- B05C11/10—Storage, supply or control of liquid or other fluent material; Recovery of excess liquid or other fluent material
- B05C11/1042—Storage, supply or control of liquid or other fluent material; Recovery of excess liquid or other fluent material provided with means for heating or cooling the liquid or other fluent material in the supplying means upstream of the applying apparatus
Landscapes
- Coating Apparatus (AREA)
Abstract
The invention provides an automatic glue pouring production line for preparing an electrophoresis gel column, a glue pouring method and application. The method comprises the following steps: the glue filling pore plate fixed with the gel glass tube is arranged in the clamping groove of the conveyor belt, the automatic glue filling production line is started, the first layer glue filling operation is carried out, the pressure glue on the gel glue surface is absorbed after the first layer glue is solidified, the second layer glue filling operation is carried out, the pressure glue on the gel glue surface is absorbed again after the second layer glue is solidified, and the electrophoresis gel column is obtained. The invention uses a plunger pump to pump two solutions quantitatively and mix the two solutions on line; through high-throughput glue pouring operation and subsequent treatment on the glue surface, the problems of poor product performance and low qualified rate in the manual operation process are solved.
Description
Technical field
The invention belongs to production equipment technology, it is related to a kind of product line, operating method and purposes, in particular to
A kind of automatic glue filling production line, glue-pouring method and purposes more particularly to a kind of automatic glue filling for being used to prepare electrophoresis coagulating rubber column gel column are raw
Producing line, glue-pouring method and purposes.
Background technique
Prior art injection process, which includes: (1), connects control cabinet control A agent (resin) and B agent (catalyst) with metering pump
Amount；(2) A agent and B agent, which are measured to be controlled by control cabinet by A metering pump and B metering pump, exports, and realizes precisely quantitative ratio
Example；(3) A metering pump connects mixing tube with B agent with the A agent that B metering pump pumps out and is mixed；(4) mixing tube outlet connection glue
Head carries out injecting glue；Mixing tube is mounted with timing system, sprays to the not used mixed glue solution of time-out from glue injecting head, prevents glue solidifying
Admittedly blocking mixing tube.
The injection process mainly mixes AB glue, treats injecting glue workpiece surface and carries out injecting glue.The concentration of glue is larger,
The characteristics of being not easy to mix, also not writing constant displacement pump exactly, therefore specially used Material sucking machine and static mixer.Static mixing device
Glue can stop, and mixed glue solution can have an impact the property of glue along with the generation of solidification during stopping；Single head
Dispenser system can only carry out injecting glue to some workpiece surfaces, and workpiece surface glue amount is few, is unable to satisfy the production of a large amount of encapsulating production lines
Amount, speed is not up to standard.
CN105880111A a kind of gas-particulate filter fine filter automatic glue filling production line and glue-pouring method, it includes master control
Cabinet processed, roller bed conveying line, station turning, encapsulating station, hand push button, online station, transfer station, fine filter, pallet, rotation
Lifting platform, compressed air line and connection cables are provided with transfer station, the filling between the station turning and encapsulating station
Transfer station is also equipped between slurry station and online station, the whole production line is using ring structure and uses roller bed conveying line
All stations are connected, a pallet is placed on each station；The side of the station turning is provided with tipper
The side of device people, the grouting station are provided with glue pouring machine.Automatic three times overturnings for realizing fine filter and four times encapsulatings.
CN207872505U discloses a kind of automatic glue filling production line of charactron, including the filling being set gradually from left to right
Adhesive dispenser, clamp device and bearing device；Glue-pouring device includes the conveyer belt driven by driving unit, if being provided on conveyer belt
The card slot for placing charactron is done, card slot is uniformly distributed along the length direction interval of conveyer belt, is provided with above conveyer belt
Automatic glue filling machine corresponding with charactron, the side wall of automatic glue filling machine are fixed on the free end of the telescopic rod of encapsulating hydraulic cylinder, fill
Glue cylinder pressure is able to drive automatic glue filling machine and moves left and right；Clamp device includes the walking dress being arranged on methods, rack on a moving belt
It sets, running gear can do straight reciprocating motion along the length direction of conveyer belt on the rack, and the lower end of running gear is affixed
There is the clamping device that can be gone up and down, bearing device is used for the charactron of support clamp device clamping.
CN100412540C discloses a kind of gradient gel film for full-automatic electrophoresis producing device method, is related to biochemical inspection
It measures and monitor the growth of standing timber the production equipment and production method of material.The present invention uses computer controlled automatic gradient gel glue process, upper and lower computer
Cooperation carries out distributed AC servo system.Glue-pouring device includes computer, and single-chip computer control system matches glue glue pouring system and can branch
Support arrangement on a small quantity when production, is needed with glue glue pouring system and can support device by tee tube mini-sized blender piping connection
Want a set of by monolithic processor controlled glue-pouring device；When a large amount of production, in the case where being controlled using a computer, as needed may be used
1~20 set of glue-pouring device of control works at the same time.Manufacturing process ingredient is how many and rate of flooding is controllable, participates in without artificial, automatically
Change degree is high.Solve that gradient gel film production is difficult, the problem of consistency difference when quality is not easy to control and batch making.It mentions
For a kind of automatic control, speed is fast, the device and method for recording gradient electrophoresis film at low cost.
The method of manual manufacture electrophoresis coagulating rubber column gel column includes: at present
(1) be intubated: we need for empty glass tube to be inserted on the encapsulating orifice plate in 100 holes, and glass tube is uniformly pressed down
Make glass tube height in the same plane.
(2) packing glue fills first layer glue: mixing glue and catalyst with liquid-transfering gun, takes after being mixed by inversion and be added in right amount
In glass tube.Glue surface is finely tuned by visual observation, is on a horizontal surface institute's sticky surface.
(3) moulding: being absorbed water using liquid-transfering gun, and hand-held liquid-transfering gun softly adds to water in first layer glue surface, and water is added to need to control
System is completed within 5min, prevents glue surface from solidifying.
(4) suction pressure glue: this single stepping needs to start to carry out after the filling of first layer glue is fond of tranquility and sets 30min, by dustless
Paper contact glue surface siphons away the moulding water that previous step pours into glue surface.
The rejection rate of manual encapsulating is higher it can be seen from above-mentioned manual process for filling colloid, appearance error and batch between batch internal difference
It is larger, be not suitable for industrialization large-scale production, and the developmental research for the automatic glue filling production line of electrophoresis coagulating rubber column gel column rarely has report
Therefore in order to meet the market demand of gel column, product quality is improved, rejection rate is reduced, needs to improve existing encapsulating work in road
The degree of automation of skill achievees the purpose that extensive steady production.
Summary of the invention
In view of the deficiencies of the prior art, the present invention intends to provide it is a kind of be used to prepare electrophoresis coagulating rubber column gel column from
Dynamic encapsulating production line, glue-pouring method and purposes.In order to meet the production demand of electrophoresis coagulating rubber column gel column, product quality is improved,
Reduce rejection rate.The present invention is quantitatively pumped into two kinds of solution, on-line mixing using plunger pump；It is operated by high-throughput encapsulating, and right
Glue surface carries out subsequent processing (glue surface leveling plus the flat glue surface of hydraulic pressure), and properties of product are poor during solving manual operation, qualification rate
Low problem, and yield is increased substantially.
To achieve this purpose, the present invention adopts the following technical scheme:
In a first aspect, the present invention provides a kind of automatic glue filling production line for being used to prepare electrophoresis coagulating rubber column gel column, the production
Line includes the first layer glue encapsulating module set gradually, water suction module and second layer glue encapsulating module according to process for filling colloid route.
The present invention is by building automatic glue filling production line, and properties of product are poor during solving manual operation, and qualification rate is low
The problem of, it is able to produce out the lipoprotein electrophoresis gel column for meeting external diagnosis reagent product requirement, and pass through production line
Automation control has achieved the purpose that produce in enormous quantities.Currently, preparing electrophoresis coagulating rubber column gel column, 4 bit manipulations using manual encapsulating mode
Work continue working 8 hours can 300 gel columns of encapsulating, but qualified gel column at most only has 200；And the present invention is used to provide
Automatic glue filling production line, 8 hours can encapsulating complete 12000, after the completion of equipment debugging, product qualification rate be can reach
100%.
Those skilled in the art can add third layer glue encapsulating module, the 4th layer of glue encapsulating module according to the encapsulating number of plies
Deng therefore, automatic glue filling production line provided by the invention includes at least two groups encapsulating module.
As currently preferred technical solution, the first layer glue encapsulating module includes the first conveyer belt and is set to
First layer glue encapsulating unit above first conveyer belt.
Preferably, the card slot for fixing encapsulating orifice plate is provided on first conveyer belt.
The first layer glue encapsulating unit includes the first adhesive injection device set gradually, leveling according to process for filling colloid route
Equipment and the first moulding equipment.
As currently preferred technical solution, first adhesive injection device includes sequentially connected charging from top to bottom
Device, mixing arrangement, the first transmission device and glue injecting head.
Preferably, the feeding device includes the first liquid storage material barrel and the second liquid storage material barrel, first liquid storage
Material barrel and the second liquid storage material barrel are separately connected the mixing arrangement.
Liquid level alarm inductor is provided in first liquid storage material barrel and the second liquid storage material barrel, for reminding liquid level
In case solution is replenished in time, avoid fluid low cause below process there is bubble, relatively cause unnecessary loss.
Preferably, it is provided on the connecting line of the first liquid storage material barrel and mixing arrangement for accurately controlling encapsulating
First conveying device of amount.
Preferably, it is provided on the connecting line of the second liquid storage material barrel and mixing arrangement for accurately controlling encapsulating
Second conveying device of amount.
Preferably, first conveying device and the second conveying device are plunger pump.
Preferably, the feeding device further includes the first water tank connecting with the mixing arrangement.
Preferably, third conveying device is provided on the connecting line of first water tank and mixing arrangement.
Preferably, the third conveying device is peristaltic pump.
Preferably, the mixing arrangement outer sheath is equipped with constant temperature heating device.
Preferably, first transmission device is the mechanical arm for driving the glue injecting head mobile.
Preferably, at least one plunger pump, the plunger pump and the injecting glue are further fixed on first transmission device
The quantity of head is identical and is correspondingly connected with.
Preferably, interim device for storing liquid, institute are additionally provided on the connecting line of the mixing arrangement and the first transmission device
It states and offers port at the top of interim device for storing liquid.The purpose of the interim liquid storage pipe is to avoid mixed frequency and subsequent encapsulating frequency
Between nuance.
Preferably, the injecting glue cephalic par is fixed with the waste liquid tank for collecting cleaning waste water.
Preferably, first adhesive injection device further includes the first control system being electrically connected with the first transmission device, is used
It is mobile in controlling the first transmission device.
During existing manual encapsulating, operator needs to mix glue and catalyst using liquid-transfering gun, rocks
It takes and is added in glass tube in right amount after mixing.Since first layer glue amount is bigger, liquid-transfering gun is needed to take twice, in the mistake of encapsulating
Liquid-transfering gun is repeatedly blown and beaten in journey, and can not accurately control the adhesive liquid quantity poured into causes glue surface height different, it is therefore desirable to operator
It is leveled by visual observation glue surface height；Pipette tips during glue are added to be easy to encounter the glass tube walls of glue surface or more,
Lead to glue wall built-up, the glue hung on inner wall is absorbed according to paper handkerchief, the paper scrap on paper handkerchief, which easily falls to glue surface, influences gel
Column quality；If not removing the glue of wall built-up, is dropped in glue-line after the solidification of wall built-up glue, also will affect product quality.Therefore, exist
When carrying out encapsulating using liquid-transfering gun, it need to guarantee the vertical liquid feeding of pipette tips, glass tube walls not touched as far as possible, when needing more costly
Between cost and monetary cost to cultivate operator carry out encapsulating operation, virtually reduce production efficiency, be also unfavorable for controlling
Production cost.
Automatic glue filling production line provided by the invention efficiently solves above-mentioned technical problem, in the first adhesive injection device, the
It is filled with A glue in one liquid storage material barrel, and is connected by the first conveying device with mixing arrangement, it is filling in the second liquid storage material barrel
There is B catalyst, the mixing of two kinds of glues is completed by mixing arrangement, one layer of heated at constant temperature dress is coated on the outside of mixing arrangement
It sets, the glue temperature for passing through mixing arrangement controls in the range of 20 ± 3 DEG C, and then controls glue setting time, thus
Improve the accuracy and controllability of encapsulating process.Mixing arrangement connects the first conveying device and the second conveying device, and connects
Controller controls mixing velocity, and the setting of speed determines by gelatin concentration；Mixture export connects interim device for storing liquid, makes
Mixed glue can have an interim buffer area, and there is an exhaust pipe in liquid storage pipe upper end, and the inside has liquid level alarm anti-
Only liquid level it is excessively high and pass through exhaust pipe overflow.The purpose of the interim device for storing liquid is to avoid mixed frequency and subsequent encapsulating frequency
Between nuance, interim device for storing liquid shape be elongated no corner angle it is cylindric, it is easy to clean.The lower end of interim device for storing liquid
Connection glue injection head.In addition setting can independently operated peristaltic pump connection water tank and mixing arrangement, entire pipeline is carried out periodically clear
It washes, prevents glue solidification damage pipe-line system.
Glue injecting head moves up and down distance and movement speed by mechanical arm control；According to 10 × 10 every rows of encapsulating orifice plate away from
From control distance of stretch out and draw back and traveling time；The tilt angle of glue injecting head is controlled by the minor rotation of mechanical arm.During encapsulating,
Since encapsulating glass tube is thinner, very high to the required precision of glue injecting head, glue injecting head will be paid special attention to during injecting glue
Micro inclination prevents drop from vacantly instilling and generates bubble.
As currently preferred technical solution, the level device includes from top to bottom sequentially connected waste liquid barrel, the
Four conveying devices, the second transmission device and leveling head.
Preferably, the 4th conveying device is peristaltic pump.
Preferably, second transmission device is the mechanical arm for driving the leveling head mobile.
Preferably, the level device further includes the second control system being electrically connected with second transmission device,
Second control system is used to control second transmission device and drops to preset height, and absorbs extra glue.
Level device is independent peristaltic pump and connects leveling head and waste liquid tank by pipeline, by being in same level
Leveling head siphons away the glue for being higher by specified value simultaneously, to reach the requirement of glue surface uniform height.Peristaltic pump connects controller
Control speed and the intermittent time of imbibition；It levels head to connect by mechanical arm, it is controlled by the second control system and is moved up and down, by
It can be solidified after a long time placement in glue, therefore level and water storage tank is installed close to equipment side at station, it is every to have leveled a plate
Glue carries out water suction operation, prevents pipeline from being solidified and blocked by glue, and leveling process reduces batch internal difference of gel column.
As currently preferred technical solution, the first moulding equipment includes from top to bottom sequentially connected second
Water tank, the 5th conveying device, third transmission device and plus head.
Preferably, second water tank and the first water tank integrated setting.
Preferably, the 5th conveying device is peristaltic pump.
Preferably, the third transmission device is the mechanical arm for driving described plus head mobile.
Preferably, the first moulding equipment further includes that the third control being electrically connected with the third transmission device is
System, it is mobile for controlling third transmission device.
Moulding process not only proposes higher requirement to operation precision, also there is stringent limitation to the operating time, on the one hand,
Glue surface solidifies in order to prevent, adds hydraulic pressure glue that control is needed to complete in 5min；On the other hand, during adding hydraulic pressure glue, glue surface
It is easy to be broken through by water, once slight jitter occurs during manual operation, is easy to cause glue surface to be layered, therefore current craft
The rejection rate of moulding link maintains 70% or so.
Moulding operation is carried out using automatic glue filling production line provided by the invention, controller is connected by peristaltic pump and controls machine
The velocity of rotation of tool arm and intermittent time, water filling head connection mechanical arm control moves up and down, inside and outside distance of stretch out and draw back and tilt angle,
Finally by controller control syringe needle tilt angle, moving distance and waterflood injection rate and water filling volume.First layer glue is still when water filling
In non-curdled appearance, add head probes into the flatness of degree meeting extreme influence glue surface, and then influences product quality, therefore should
Process is higher to the required precision of equipment, and inclination is adherent to be moved down, and the depth for contacting liquid level is less than 1mm.As long as guaranteeing that equipment is stablized
Operation, the success rate of moulding link is up to 100%.
As currently preferred technical solution, the water suction module includes pedestal and is set to above the pedestal
Station fixed station is provided with the card slot for fixing encapsulating orifice plate on the station fixed station.
Preferably, inclination is fixed at an angle between the station fixed station and the pedestal, it is further preferred that institute
Station fixed station is stated to be fixed on the pedestal in 45° angle inclination.
Preferably, suction head, the 4th transmission device, the 6th defeated is disposed with above the station fixed station from the bottom to top
Send device and waste tank.
Preferably, the 4th transmission device is the mechanical arm for driving the suction head mobile.
Preferably, the 6th conveying device is peristaltic pump.
Preferably, the suction head is arranged vertically with the station fixed station.
Preferably, the water suction module further includes the 4th control system being electrically connected with the 4th transmission device, is used
In the water suction route and residence time that control the 4th transmission device.
Existing craft encapsulating absorbs extra moulding water using dust-free paper, and one side moulding water can not be absorbed completely, be led
It causes glue when second layer glue encapsulating to be diluted by remaining moulding water, influences the performance of gel column；On the other hand, in water absorption course,
Dust-free paper once falls bits, and gel column is scrapped immediately, causes heavy losses to production cost.
Water absorber in automatic glue filling production line provided by the invention is separating apparatus, suction head and working-position vertical,
Entirety and desktop facilitate residual water by gravity and focus on minimum point, it is accurate that mechanical arm controls suction head at 45 degree of overturning angles
Positioning, and the minimum point that slowly touching glue surface intersects with gel glass pipe, utmostly absorb residual water.The present invention passes through automatic
Change substitution manual operations, avoids the manual operations problem low because of gel column yield rate caused by shaking, while using suction head
Dust-free paper is replaced, and encapsulating orifice plate is tilted and is fixed, is more advantageous to and absorbs moulding water, also solve the problems, such as dust-free paper falls bits.
As currently preferred technical solution, the second layer glue encapsulating module includes the second conveyer belt and is set to institute
State the second layer glue encapsulating unit above the second conveyer belt.
Preferably, the second layer glue encapsulating unit includes that sequentially connected second encapsulating is set according to process for filling colloid route
Standby and the second moulding equipment.
Preferably, second adhesive injection device is identical as first adhesive injection device.
Preferably, the second moulding equipment is identical as the first moulding equipment.
Second layer glue encapsulating module is similar with first layer glue encapsulating module, and level device has been lacked in centre.Wherein glue amount and when
Between by process control.
The equipment time out of service is more than 10 minutes during encapsulating, need to carry out pipeline cleaning work to pipeline, open clear
Washing procedure, peristaltic pump connect bucket and mixing arrangement, clean since the place that glue is mixed with catalyst, waste liquid passes through injecting glue
Head navigates to above waste liquid tank, and waste liquid tank is connected to external waste liquid barrel by pipeline.
Second aspect, the present invention provides a kind of glue-pouring method of electrophoresis coagulating rubber column gel column, the method is described in the first aspect
Production line in carry out.
Preferably, the glue-pouring method includes:
The encapsulating orifice plate for being fixed with gel glass pipe is placed in the card slot of the conveyer belt, opens automatic glue filling production line,
The operation of first layer glue encapsulating is carried out, the moulding water in gel glue surface is absorbed after first layer gelling is solid, carries out second layer glue encapsulating behaviour
Make, absorbs the moulding water in gel glue surface again after second layer gelling is solid, obtain the electrophoresis coagulating rubber column gel column.
Illustratively, the present invention provides a kind of optional technical solution, the glue-pouring method includes:
(1) encapsulating: 100 gel glass pipes are fixed on 10 × 10 encapsulating orifice plate, band of the glue injecting head in mechanical arm
It is accurately positioned under dynamic, encapsulating amount, the corresponding plunger pump of each glue injecting head is controlled by plunger pump, encapsulating starts rear injecting glue
Head reaches the gel glass pipe of encapsulating orifice plate most one row of edge, and encapsulating operates since first row, has successively filled 10 rows.
(2) level: after the completion of encapsulating, the first conveyer belt by glue filling opening strip to leveling workshop section, while to encapsulating workshop section sky
Scarce slot supplements new encapsulating orifice plate and continues encapsulating workshop section operational order.Second operation work start to gel face into
Row leveling sets ten holes leveling head by the second control system and drops to fixed height, and controls mechanical arm movement, drives and adjusts
Tack drops to predeterminated position, and extra glue is absorbed under the action of peristaltic pump, and scrap rubber liquid flows into waste liquid barrel by pipeline.It should
The purpose of process is to adjust 100 gel column glue surfaces to uniform height.
(3) moulding: after the completion of leveling, for the first conveyer belt by glue filling opening strip to moulding workshop section of dripping, first two steps process is same
When the corresponding course of work of repetitive operation.Third control system is inserted perpendicularly into gel glass pipe by mechanical arm control plus head
In, the flow of moulding water is controlled by peristaltic pump, patch glass inside pipe wall is along glue surface pressurization glue.The step is entire encapsulating stream
A most important link in journey, so that the precision of equipment has accomplished to destroy the minimum of glue surface, moulding success rate is high.
(4) it absorbs water: absorbing the moulding water on gel face after first layer gelling is solid, concrete operation step includes: that suction head exists
It under the drive of mechanical arm, is probeed into gel glass pipe along the direction vertical with station fixed station, is close to glass inside pipe wall and slowly connects
Glue surface is touched, in the progradation of suction head, moulding water flows to minimum point under gravity, and suction head stays 2 seconds, and leads to
It crosses peristaltic pump and absorbs extra moulding water, moulding water is collected by pipeline to waste tank.It absorbs water well route according to program setting
Ecto-entad is repeated in the water suction operation that 100 gel glass pipes are completed in identical movement.
(5) second layer glue encapsulating process: similar with first layer glue process, leveling step has been lacked in centre.Wherein glue amount and when
Between by process control.
(6) it absorbs water: absorbing the moulding water on gel face after first layer gelling is solid again, concrete operation step and step (4)
It is identical.
The third aspect, the present invention provides a kind of electrophoresis coagulating rubber column gel column, the electrophoresis coagulating rubber column gel column is described in the first aspect
It is prepared in production line.
Fourth aspect, the present invention provides a kind of purposes of production line described in first aspect, the production line is for electricity
The automatic glue filling of swimming gel column.
Preferably, the electrophoresis coagulating rubber column gel column is lipoprotein electrophoresis gel column.
Numberical range of the present invention not only includes enumerated point value, further includes the above-mentioned numerical value not included
Arbitrary point value between range, as space is limited and for concise consideration, range described in the present invention no longer exclusive list includes
Specific point value.
Compared with prior art, the invention has the benefit that
In order to meet the production demand of electrophoresis coagulating rubber column gel column, product quality is improved, rejection rate is reduced.The present invention uses
Plunger pump is quantitatively pumped into two kinds of solution, on-line mixing；It is operated by high-throughput encapsulating, and subsequent processing (glue surface is carried out to glue surface
Leveling plus the flat glue surface of hydraulic pressure), properties of product are poor during solving manual operation, the low problem of qualification rate.
Automatic glue filling production line provided by the invention is able to produce out the lipoprotein for meeting external diagnosis reagent product requirement
Electrophoresis coagulating rubber column gel column, and by the automation control of production line, achieve the purpose that produce in enormous quantities.
Detailed description of the invention
Fig. 1 is the first layer glue encapsulating modular structure schematic diagram that the embodiment of the present invention 1 provides；
Fig. 2 is the water suction modular structure schematic diagram that the embodiment of the present invention 1 provides；
Fig. 3 is the second layer glue encapsulating modular structure schematic diagram that the embodiment of the present invention 1 provides；
Wherein: 1,31- first liquid storage material barrel；2,32- second liquid storage material barrels；3,33- water tanks；4,5,12,34,35,
42- plunger pump；6,36,22,29,47- peristaltic pumps；7,37- mixing arrangements；8,38- constant temperature heating devices；9,39- ports；10,
The interim device for storing liquid of 40-；11,19,23,28,41,48- mechanical arms；13,43- glue injecting heads；14,44- waste liquid tanks；15,45- cards
Slot；The first conveyer belt of 16-；17- waste liquid barrel；18- peristaltic pump；20- levels head；21- water storage tank；24,49- add head；25- base
Seat；26- station fixed station；27- suction head；30- waste tank；The second conveyer belt of 46-.
Specific embodiment
To further illustrate the technical scheme of the present invention below with reference to the accompanying drawings and specific embodiments.
Embodiment 1
A kind of automatic glue filling production line for being used to prepare electrophoresis coagulating rubber column gel column is present embodiments provided, the production line is according to filling
Adhesive process route includes the first layer glue encapsulating module set gradually, water suction module and second layer glue encapsulating module.
The structure of first layer glue encapsulating module is as shown in Figure 1, include that the first conveyer belt 16 is transmitted with being set to described first
First layer glue encapsulating unit with 16 tops；The card slot 15 for fixing encapsulating orifice plate is provided on first conveyer belt 16；First
Layer glue encapsulating unit includes that the first adhesive injection device, level device and the first moulding set gradually is set according to process for filling colloid route
It is standby.
First adhesive injection device includes from top to bottom sequentially connected feeding device, mixing arrangement 7, mechanical arm 11 and glue injecting head
13.Wherein, feeding device includes the first liquid storage material barrel 1 and the second liquid storage material barrel connecting respectively with the mixing arrangement 7
2, the plunger pump 4 for accurately controlling encapsulating amount is provided on the first liquid storage material barrel 1 and the connecting line of mixing arrangement 7, the
The plunger pump 5 for accurately controlling encapsulating amount is provided on two liquid storage material barrels 2 and the connecting line of the mixing arrangement 7.Into
Material device further includes the water tank 3 connecting with mixing arrangement 7, is provided with peristaltic pump 6 on water tank 3 and the connecting line of mixing arrangement 7.
7 outer sheath of mixing arrangement is equipped with constant temperature heating device 8.At least one plunger pump 12 is further fixed on mechanical arm 11, illustratively,
10 plunger pumps 12, each plunger pump 12 are fixed on mechanical arm 11 shown in first layer glue encapsulating module as described in Figure 1
On be respectively connected with glue injecting head 13,13 end of glue injecting head is fixed with the waste liquid tank 14 for collecting cleaning waste water.Mixing arrangement 7 and machine
It is additionally provided with interim device for storing liquid 10 on the connecting line of tool arm 11, offers port 9 at the top of interim device for storing liquid 10.Described
First adhesive injection device further includes the first control system being electrically connected with mechanical arm 11, and be used to control mechanical arm 11 mobile.
Level device includes sequentially connected waste liquid barrel 17 from top to bottom, peristaltic pump 18, mechanical arm 19 and leveling head 20；Institute
The level device stated further includes the second control system being electrically connected with mechanical arm 19, and second control system is for controlling machine
Tool arm 19 drops to preset height, absorbs extra glue.
First moulding equipment includes sequentially connected second water tank, peristaltic pump 22, mechanical arm 23 and adds head 24.In one kind
In preferred technical solution, as shown in Figure 1, second water tank and the first adhesive injection device share same water tank 3.First moulding is set
Standby further includes third control system, and the third control system drops to preset height for controlling mechanical arm 23.
The structure for the module that absorbs water is as shown in Fig. 2, include pedestal 25 and the station fixed station for being set to 25 top of pedestal
26, the card slot for fixing encapsulating orifice plate is provided on station fixed station 26, station fixed station 26 is fixed in 45 degree of overturning angles
On the pedestal 25.26 top of station fixed station is disposed with suction head 27, mechanical arm 28, peristaltic pump 29 from the bottom to top and gives up
Liquid storage tank 30, the suction head 27 are arranged vertically with station fixed station 26.The water suction module further includes the 4th control system, institute
The 4th control system is stated for controlling water suction route and the residence time of the mechanical arm 28.
The structure of second layer glue encapsulating module is as shown in figure 3, include that the second conveyer belt 46 is transmitted with being set to described second
Second layer glue encapsulating unit with 46 tops；The card slot 45 for fixing encapsulating orifice plate is provided on first conveyer belt 46；
The second layer glue encapsulating unit includes that sequentially connected second adhesive injection device and the second moulding are set according to process for filling colloid route
It is standby.
Second adhesive injection device is identical as above-mentioned first adhesive injection device, as shown in figure 3, second adhesive injection device
Including sequentially connected feeding device from top to bottom, mixing arrangement 37, mechanical arm 41 and glue injecting head 43.Wherein, feeding device packet
Include the first liquid storage material barrel 31 and the second liquid storage material barrel 32 connecting respectively with the mixing arrangement 37, the first liquid storage material barrel
31 with the plunger pump 34 for accurately controlling encapsulating amount, the second liquid storage material barrel 32 are provided on the connecting line of mixing arrangement 37
With the plunger pump 35 for accurately controlling encapsulating amount is provided on the connecting line of the mixing arrangement 37.Feeding device further includes
Peristaltic pump 36 is provided on the connecting line of the water tank 33 connecting with mixing arrangement 37, water tank 33 and mixing arrangement 7.Mixing arrangement
37 outer sheaths are equipped with constant temperature heating device 38.At least one plunger pump 42 is further fixed on mechanical arm 41, illustratively, such as Fig. 3
10 plunger pumps 42 are fixed on mechanical arm 41 shown in the second layer glue encapsulating module, are connected on each plunger pump 42
It is connected to glue injecting head 43,43 end of glue injecting head is fixed with the waste liquid tank 44 for collecting cleaning waste water.Mixing arrangement 37 and mechanical arm
It is additionally provided with interim device for storing liquid 40 on 41 connecting line, offers port 39 at the top of interim device for storing liquid 40.Described
One adhesive injection device further includes the first control system being electrically connected with mechanical arm 41, and be used to control mechanical arm 41 mobile.
The second moulding equipment is identical as above-mentioned first moulding equipment, including sequentially connected second water tank, wriggling
Pump 47, mechanical arm 48 and plus head 49.In a preferred technical solution, as shown in figure 3, second water tank and the second layer
First adhesive injection device of glue encapsulating module shares same water tank 33.First moulding equipment further includes third control system, and described
Three control systems drop to preset height for controlling mechanical arm 48.
Application Example 1
Lipoprotein electrophoresis gel column is produced using the automatic glue filling production line that embodiment 1 provides, the operating procedure includes:
First layer glue encapsulating, for the first time water suction, second layer glue encapsulating and second absorb water.
Wherein, first layer glue encapsulating includes:
(1) encapsulating: the filling A glue into the first liquid storage material pot, the filling B catalyst into the second liquid storage material pot, by 100
A gel glass pipe is fixed on 10 × 10 encapsulating orifice plate, and glue injecting head 13 is accurately located to first under the drive of mechanical arm 11
Condensate drain glue glass tube controls encapsulating amount, the corresponding gel glass pipe of each glue injecting head, since first row by plunger pump 12
Encapsulating operation, has successively filled 10 rows.
(2) level: after the completion of encapsulating process, the first conveyer belt 16 drives encapsulating orifice plate to be moved to leveling process, while to
The slot of encapsulating process vacancy supplements new encapsulating orifice plate and continues encapsulating operational order.Leveling process starts to gel
Face is leveled, and controls the movement of mechanical arm 19 by the second control system, mechanical arm 19 drives leveling head 20 to drop to default position
It sets, the extra glue for being higher than preset height is absorbed under the action of peristaltic pump 18, scrap rubber liquid flows into waste liquid barrel 17 by pipeline.
(3) moulding: after the completion of leveling process, the first conveyer belt 16 drives encapsulating orifice plate to be moved to moulding process of dripping, preceding
Two step processes while the corresponding workflow of repetitive operation.Third control system is vertically inserted by the control of mechanical arm 23 plus head 24
Entering in gel glass pipe, the flow of moulding water is controlled by peristaltic pump 22, the inner wall test for being close to gel glass pipe adds head 24,
And along glue surface pressurization glue.
It absorbs water for the first time: absorbing the moulding water on gel face after first layer gelling is solid, suction head 27 is in mechanical arm 28
It under drive, is probeed into gel glass pipe along the direction vertical with station fixed station 26, is close to gel glass inside pipe wall and slowly contacts
Glue surface, in the progradation of suction head 27, moulding water flows to minimum point under gravity, and suction head 27 stays 2 seconds,
Extra moulding water is absorbed under the action of peristaltic pump 29, moulding water is collected by pipeline to waste tank 30.It is set according to program
It sets water suction route ecto-entad and is repeated in the water suction operation that 100 gel glass pipes are completed in identical movement.
Second layer glue encapsulating is similar with first layer glue encapsulating process, and difference is, has lacked the leveling process of step (2).
Second of water suction: the moulding water on gel face, concrete operation step and first are absorbed after second layer gelling is solid again
Secondary water suction process is identical.
The adhesive injection device time out of service is more than 10 minutes during encapsulating, need to carry out pipeline cleaning work to pipeline, open
Logical cleaning procedure, peristaltic pump 6 connect bucket 3 and continuous mixing device 7, clean since the place that glue is mixed with catalyst, give up
Liquid navigates to 13 top of waste liquid tank by glue injecting head 12, and the waste water after cleaning is temporarily collected by waste liquid tank 13.
Bondline thickness is observed the appearance for 100 electrophoresis coagulating rubber column gel columns being prepared and measured, test data is recorded
It is shown in Table 1.
Table 1
Standard deviation (SD), the average value of the appearance data of 100 electrophoresis coagulating rubber column gel columns are calculated according to above-mentioned appearance test data
(mean) and internal difference (CV%) is criticized, calculated result is shown in Table 2.
Table 2
| First layer glue bondline thickness | Second layer glue bondline thickness | |
| SD | 0.42 | 0.37 |
| mean | 55.2 | 6.7 |
| CV% | 0.76 | 5.5 |
36 gel column samples are randomly selected in above-mentioned 100 electrophoresis coagulating rubber column gel columns, and randomly select 2 parts of test sample marks
It is denoted as sample 1 and sample 2, using human plasma or serum as test sample, by lipoprotein dyeing liquor to the lipoprotein in blood plasma
It is coloured, sample 1 and sample 2 is added into the upper surface of gel column second layer glue respectively, carry out electrophoresis experiment.Shape after electrophoresis
Software is carried out to test result and analyzes to obtain test data by scanning at a series of bands.
18 lipoprotein electrophoresis experiments are repeated to sample 1 and sample 2, the sub- parting after recording lipoprotein electrophoresis, packet
Include triglycerides (T-C), low-density lipoprotein (LDL-C), very low density lipoprotein (VLDL), medium density lipoprotein (MID-C)
With the content of high-density lipoprotein (HDL), and calculate 18 times repetition experiment standard deviation (SD), average value (mean) and criticize internal differences
(CV%), the test result of sample 1 is referring to table 3, and the test result of sample 2 is referring to table 4.
Table 3
Table 4
Comparative example 1
Comparative example 1 uses manual encapsulating operation preparation electrophoresis coagulating rubber column gel column, includes the following steps:
(1) it is intubated: empty glass tube being inserted on encapsulating orifice plate, glass tube, which is uniformly pressed down, makes glass tube height same
In one plane；The last at most fixed two rows of glass tubes of 10 × 10 encapsulating orifice plate, need between two rows of glass tubes to separate 2 rows with
On, i.e., at most fix 20 glass tubes；
(2) packing glue fills first layer glue: mixing glue and catalyst with liquid-transfering gun, takes after being mixed by inversion and be added in right amount
In glass tube, encapsulating twice, repeatedly blow and beat during encapsulating by liquid-transfering gun, and every mixing once fills 10 gel columns, passes through view
Feel whether observation glue surface is horizontal, and carries out Levelling operation；
(3) moulding: using peristaltic pump, and hand-held needle softly adds to water in first layer glue surface, and water is added to need to control
It is completed within 5min, prevents glue surface from solidifying；
(4) suction pressure glue: 30min is stood after first layer glue encapsulating and waits for that first layer glue solidifies completely, use is small-sized
Vacuum pump connects pipette tips and takes out negative pressure, holds pipette tips, contacts the moulding water that glue surface siphons away previous step moulding injection in sequence；
(5) second layer glue encapsulating: step (1)-(4) are repeated and complete the operation of second layer glue encapsulating.
Bondline thickness is observed and measured to the appearance of the electrophoresis coagulating rubber column gel column qualified to 100 be prepared, and record is simultaneously
The glue-line standard deviation (SD) of 100 electrophoresis coagulating rubber column gel columns, average value (mean) and crowd internal difference (CV%) are calculated, calculated result is shown in Table 5.
Table 5
| First layer glue bondline thickness | Second layer glue bondline thickness | |
| SD | 0.45 | 0.42 |
| mean | 55.2 | 6.7 |
| CV% | 0.82 | 6.2 |
18 gel column samples are randomly selected in above-mentioned 100 electrophoresis coagulating rubber column gel columns, and randomly select 1 part of test sample mark
It is denoted as sample 3, using human plasma or serum as test sample, the lipoprotein in blood plasma is carried out by lipoprotein dyeing liquor
Sample 3 is added to the upper surface of gel column sample second layer glue, carries out electrophoresis experiment by color.A series of bands are formed after electrophoresis,
By scanning, software is carried out to test result and analyzes to obtain test data.
18 lipoprotein electrophoresis experiments are repeated to sample 3, the sub- parting after recording lipoprotein electrophoresis, including glycerol three
Ester (T-C), low-density lipoprotein (LDL-C), very low density lipoprotein (VLDL), medium density lipoprotein (MID-C) and high density
The content of lipoprotein (HDL), and calculate 18 times repetition experiment standard deviation (SD), average value (mean) and criticize internal difference (CV%),
Test result is referring to table 6.
Table 6
Summary analysis 2 and table 5 can be seen that the electrophoresis coagulating rubber column gel column of automatic glue filling production line production provided by the invention
The standard deviation of appearance data and batch internal difference are respectively less than manual encapsulating, it is possible thereby to illustrate automatic glue filling production line provided by the invention
The appearance of the electrophoresis coagulating rubber column gel column of production is more more stable than manual encapsulating.
The automatic glue filling production line provided by the invention it can be seen from every electrophoresis test data in table 3, table 4 and table 6
The standard deviation of the electrophoresis test data of the electrophoresis coagulating rubber column gel column of production and batch internal difference are respectively less than manual encapsulating, it is possible thereby to illustrate this hair
Properties of product of the bright electrophoresis coagulating rubber column gel column being prepared during lipoprotein electrophoresis are relatively stable, are suitble to extensive batch raw
It produces.
The Applicant declares that the foregoing is merely a specific embodiment of the invention, but protection scope of the present invention not office
It is limited to this, it should be clear to those skilled in the art, any to belong to those skilled in the art and take off in the present invention
In the technical scope of dew, any changes or substitutions that can be easily thought of, and all of which fall within the scope of protection and disclosure of the present invention.
Claims (10)
1. a kind of automatic glue filling production line for being used to prepare electrophoresis coagulating rubber column gel column, which is characterized in that the production line is according to encapsulating work
Skill route includes the first layer glue encapsulating module set gradually, water suction module and second layer glue encapsulating module.
2. production line according to claim 1, which is characterized in that the first layer glue encapsulating module includes the first transmission
Band and the first layer glue encapsulating unit being set to above first conveyer belt；
Preferably, the card slot for fixing encapsulating orifice plate is provided on first conveyer belt；
The first layer glue encapsulating unit includes the first adhesive injection device, the level device set gradually according to process for filling colloid route
With the first moulding equipment.
3. production line according to claim 2, which is characterized in that first adhesive injection device includes from top to bottom successively
Feeding device, mixing arrangement, the first transmission device and the glue injecting head of connection；
Preferably, the feeding device includes the first liquid storage material barrel and the second liquid storage material barrel, the first liquid storage material
Bucket and the second liquid storage material barrel are separately connected the mixing arrangement；
Preferably, it is provided on the connecting line of the first liquid storage material barrel and mixing arrangement for accurately controlling encapsulating amount
First conveying device；
Preferably, it is provided on the connecting line of the second liquid storage material barrel and mixing arrangement for accurately controlling encapsulating amount
Second conveying device；
Preferably, first conveying device and the second conveying device are plunger pump；
Preferably, the feeding device further includes the first water tank connecting with the mixing arrangement；
Preferably, third conveying device is provided on the connecting line of first water tank and mixing arrangement；
Preferably, the third conveying device is peristaltic pump；
Preferably, the mixing arrangement outer sheath is equipped with constant temperature heating device；
Preferably, first transmission device is the mechanical arm for driving the glue injecting head mobile；
Preferably, at least one plunger pump, the plunger pump and the glue injecting head are further fixed on first transmission device
Quantity is identical and is correspondingly connected with；
Preferably, interim device for storing liquid is additionally provided on the connecting line of the mixing arrangement and the first transmission device, it is described to face
When device for storing liquid at the top of offer port；
Preferably, the injecting glue cephalic par is fixed with the waste liquid tank for collecting cleaning waste water；
Preferably, first adhesive injection device further includes the first control system being electrically connected with the first transmission device, for controlling
It is mobile to make the first transmission device.
4. production line according to claim 2 or 3, which is characterized in that the level device includes from top to bottom successively connecting
Waste liquid barrel, the 4th conveying device, the second transmission device and the leveling head connect；
Preferably, the 4th conveying device is peristaltic pump；
Preferably, second transmission device is the mechanical arm for driving the leveling head mobile；
Preferably, the level device further includes the second control system being electrically connected with second transmission device, described
Second control system is used to control second transmission device and drops to preset height, and absorbs extra glue.
5. according to the described in any item production lines of claim 2-4, which is characterized in that the first moulding equipment includes by upper
Sequentially connected second water tank, the 5th conveying device, third transmission device and plus head under；
Preferably, second water tank and the first water tank integrated setting；
Preferably, the 5th conveying device is peristaltic pump；
Preferably, the third transmission device is the mechanical arm for driving described plus head mobile；
Preferably, the first moulding equipment further includes the third control system being electrically connected with the third transmission device,
It is mobile for controlling third transmission device.
6. production line according to claim 1-5, which is characterized in that the water suction module includes pedestal and sets
The station fixed station being placed in above the pedestal is provided with the card slot for fixing encapsulating orifice plate on the station fixed station；
Preferably, inclination is fixed at an angle between the station fixed station and the pedestal, it is further preferred that the work
Position fixed station is fixed on the pedestal in 45° angle inclination；
Preferably, suction head, the 4th transmission device, the 6th conveying dress are disposed with above the station fixed station from the bottom to top
It sets and waste tank；
Preferably, the 4th transmission device is the mechanical arm for driving the suction head mobile；
Preferably, the 6th conveying device is peristaltic pump；
Preferably, the suction head is arranged vertically with the station fixed station；
Preferably, the water suction module further includes the 4th control system being electrically connected with the 4th transmission device, for controlling
Make water suction route and the residence time of the 4th transmission device.
7. production line according to claim 1-6, which is characterized in that the second layer glue encapsulating module includes the
Two conveyer belts and the second layer glue encapsulating unit being set to above second conveyer belt；
Preferably, the second layer glue encapsulating unit according to process for filling colloid route include sequentially connected second adhesive injection device and
Second moulding equipment；
Preferably, second adhesive injection device is identical as first adhesive injection device；
Preferably, the second moulding equipment is identical as the first moulding equipment.
8. a kind of glue-pouring method of electrophoresis coagulating rubber column gel column, which is characterized in that the method is in the described in any item lifes of claim 1-7
It is carried out in producing line；
Preferably, the glue-pouring method includes:
The encapsulating orifice plate for being fixed with gel glass pipe is placed in the card slot of the conveyer belt, opens automatic glue filling production line, is carried out
The moulding water in gel glue surface is absorbed in the operation of first layer glue encapsulating after first layer gelling is solid, carry out the operation of second layer glue encapsulating, to
The moulding water in gel glue surface is absorbed after second layer gelling is solid again, obtains the electrophoresis coagulating rubber column gel column.
9. a kind of electrophoresis coagulating rubber column gel column, which is characterized in that the electrophoresis coagulating rubber column gel column is in the described in any item productions of claim 1-7
It is prepared in line.
10. a kind of purposes of the described in any item production lines of claim 1-7, which is characterized in that the production line is used for electrophoresis
The automatic glue filling of gel column；
Preferably, the electrophoresis coagulating rubber column gel column is lipoprotein electrophoresis gel column.
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| CN110920107A (en) * | 2019-12-23 | 2020-03-27 | 苏州博古特智造有限公司 | Lens production device and lens production method |
| CN111204065A (en) * | 2020-01-14 | 2020-05-29 | 苏州瑞意达机械设备有限公司 | Synchronous glue discharging and mixing control system |
| CN111420844A (en) * | 2020-03-25 | 2020-07-17 | 南京精益通信息技术有限公司 | Control system and control method for electronic product production |
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| CN110920107A (en) * | 2019-12-23 | 2020-03-27 | 苏州博古特智造有限公司 | Lens production device and lens production method |
| CN111204065A (en) * | 2020-01-14 | 2020-05-29 | 苏州瑞意达机械设备有限公司 | Synchronous glue discharging and mixing control system |
| CN111420844A (en) * | 2020-03-25 | 2020-07-17 | 南京精益通信息技术有限公司 | Control system and control method for electronic product production |
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| CN109590170B (en) | 2020-02-21 |
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