CN215712982U

CN215712982U - Pregnant woman premature delivery prediction device based on high-throughput sequencing data

Info

Publication number: CN215712982U
Application number: CN202121025365.1U
Authority: CN
Inventors: 漆洪波; 尹楠林; 冉雨鑫
Original assignee: First Affiliated Hospital of Chongqing Medical University
Current assignee: First Affiliated Hospital of Chongqing Medical University
Priority date: 2021-05-13
Filing date: 2021-05-13
Publication date: 2022-02-01
Anticipated expiration: 2031-05-13

Abstract

The utility model relates to a pregnant woman premature delivery prediction device based on high-throughput sequencing data, which relates to the technical field of gene sequencing, and comprises a work box, a placement device, an RNA extraction device, a detection device, a drip suction device and an analysis device, wherein the work box comprises a box body, the placement device comprises a first vertical rod, a transverse rail, a second vertical rod and a longitudinal rail, the RNA extraction device comprises vertical rails and a first sliding seat, one end of each of the two first vertical rails is connected with the inner peripheral side surface of the box body, the detection device comprises a sequencer, the drip suction device comprises a base and a connecting seat, and the analysis device comprises a mounting plate, an analyzer and a connecting wire; and finally, a statistical model built in an analysis system is introduced, and is compared and analyzed with the previously stored big data, so that the risk of premature delivery and the delivery time are evaluated.

Description

Pregnant woman premature delivery prediction device based on high-throughput sequencing data

Technical Field

The utility model relates to the technical field of gene sequencing, in particular to a pregnant woman premature delivery prediction device based on high-throughput sequencing data.

Background

Circular RNA is a novel non-coding RNA, and due to its unique circular structure and temporal specificity, its predictive, diagnostic value as a biomarker in a variety of diseases is considered to far exceed that of traditional linear RNA. In the early stage of the group, high-throughput sequencing data of a GEO database are used for the first time to identify the circRNA in the peripheral blood of the pregnant women in premature delivery and full term (all patients are single pregnancy, non-preeclampsia, fetal growth limitation, fetal distress and other pregnancy complications; a premature group (less than 37 weeks) shows symptoms and signs of spontaneous premature delivery, such as regular uterine contraction; and the analysis result proves that the change of the circular RNA is closely related to the occurrence of premature delivery no matter whether the group is in production or not, and further, a small-range verification experiment is carried out, and the fact that the circRNA has the unique potential as a novel biomarker for predicting and diagnosing premature delivery is proposed for the first time.

The method exactly predicts the premature birth risk and the delivery date of the pregnant woman, is important for effectively implementing clinical intervention and guaranteeing the life and health of the pregnant woman, and with the progress of science and technology, a new theory for accurately predicting the premature birth risk through a circular RNA molecule based on high-throughput sequencing data and a scientific systematic analysis method is provided.

The pregnant woman premature delivery prediction device in the prior art has the problems of poor clinical feasibility, high risk of errors in placement and transfer of a sampling tube, complex detection process and the like, and therefore a pregnant woman premature delivery prediction device based on high-throughput sequencing data is provided.

SUMMERY OF THE UTILITY MODEL

The utility model mainly aims to provide a pregnant woman premature delivery prediction device based on high-throughput sequencing data, which can effectively solve the problems of poor clinical feasibility, high risk of errors in placement and transfer of a sampling tube, complicated detection process and the like in the background technology.

In order to solve the technical problems, the utility model is realized by the following technical scheme: a pregnant woman premature delivery prediction device based on high-throughput sequencing data comprises a work box, a placing device, an RNA extraction device, a detection device, a drip absorption device and an analysis device, wherein the work box comprises a box body which is of a hollow structure, the placing device comprises a first vertical rod, a transverse rail, a second vertical rod and a longitudinal rail, one end of each transverse rail is connected with the lower surface of the inner portion of the box body, the other end of each transverse rail is in sliding fit with the two first vertical rods respectively, one end of each longitudinal rail is connected with the lower surface of the inner portion of the box body, the other end of each longitudinal rail is in sliding fit with the two second vertical rods respectively, the RNA extraction device comprises a vertical rail and a first sliding seat, one end of each first vertical rail is connected with the inner circumferential side face of the box body, the other end of each vertical rail is in sliding fit with the two first sliding seats respectively, the detection device comprises a sequencer, sequencer lower surface and box inside lower surface are connected, it includes base and connecting seat to inhale and drip the device, base one end and box inside upper surface are connected, the base other end and two connecting seat upper surface are connected, analytical equipment includes mounting panel, analysis appearance and connecting wire, the connecting wire sets up between sequencer and analysis appearance, mounting panel week side and box outside week side are connected.

The working box further comprises a rotating shaft, a box cover, a handle and a disinfection lamp, one end of the rotating shaft is in rotating fit with the box body, the other end of the rotating shaft is in rotating fit with the box cover, the handle is installed on the peripheral side face of the box cover, the disinfection lamp is installed on the upper surface inside the box body, and the disinfection lamp can disinfect the inside of the box body in real time.

The placing device comprises a first placing plate, a second placing plate and a fixing frame, wherein the fixing frame is respectively installed on one surface of each longitudinal rail, the fixing frame is connected with the circumferential side of the first placing plate, the circumferential side of the second placing plate is installed on the circumferential side of the second placing plate, the second placing plate can be fixed by the fixing frame, and the drip sucking device is convenient to work.

RNA extraction element still includes balladeur train, second slide and full-automatic extraction appearance, balladeur train one end is connected with two first slide week sides respectively, the balladeur train other end and second slide sliding fit, full-automatic extraction appearance is installed in second slide week side, and sliding fit can change the upper and lower position of full-automatic extraction appearance between first slide and the founding rail.

The detection device further comprises a rotary column, rotary seats and a cover plate, wherein the rotary seats are arranged on the upper surface of the sequencer, the rotary column is in rotary fit with the two rotary seats respectively, the cover plate is in rotary fit with the rotary column, and sequencing operation is started after the sequencer is tightly covered by the cover plate in a rotating mode.

Inhale and drip device still includes head rod, first column spinner, second connecting rod, second column spinner and inhales and drips the ware, first column spinner one end respectively with two connecting seats normal running fit, the first column spinner other end and head rod normal running fit, the head rod passes through second column spinner normal running fit with the second connecting rod, inhale and drip the ware and install in second connecting rod lower surface, inhale and drip the ware and place the sample absorption in the board upper test tube with the second and drip into in the sequencer.

The analysis device further comprises a straight rod, a display screen and a control panel, the control panel is electrically connected with the analyzer through a wire, the analyzer is installed on the upper surface of the installation plate, the straight rod is arranged between the analyzer and the display screen, and the control panel can control the first board placing and the second board placing to enter, exit, start, pause, stop and other commands and monitor the degree of the internal task.

According to the utility model, the disposable sampling tube is placed on the first placing plate, then the extracted RNA solution is placed in the test tube in the second placing plate by the full-automatic extractor, then the second vertical rod and the longitudinal rail slide to the position of the fixing frame and stop, the RNA solution is absorbed by the drip absorbing device in the drip absorbing device and dripped into the sequencer, the cover plate is tightly covered to perform sequencing work, the analyzer analyzes the result in time and displays the result on the display screen, the whole sequencing analysis process is simple and rapid, and the clinical feasibility is strong; sliding fit between first pole setting and horizontal rail is convenient to place the disposable sampling pipe after gathering at the first board of placing, and sliding fit can change the second in a flexible way between second pole setting and the vertical rail and place board and detection device's position, and both cooperate and make things convenient for drawing and shifting of sample more, also make the detection flow become simple swift simultaneously.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used for describing the embodiments will be briefly introduced below.

FIG. 1 is a schematic diagram of the overall structure of a device for predicting premature delivery of pregnant women based on high-throughput sequencing data according to the present invention;

FIG. 2 is a schematic diagram of the overall structure of a device for predicting premature delivery of pregnant women based on high-throughput sequencing data according to the present invention

FIG. 3 is a schematic diagram of an elevational view of a preterm delivery prediction apparatus for pregnant women based on high-throughput sequencing data according to the present invention;

FIG. 4 is a schematic diagram of a top view of a preterm delivery prediction apparatus for pregnant women based on high-throughput sequencing data according to the present invention;

fig. 5 is a schematic side view of the pregnant woman preterm delivery prediction device based on high-throughput sequencing data.

In the drawings, the components represented by the respective reference numerals are listed below:

100. a work box; 110. a box body; 120. a rotating shaft; 130. a box cover; 140. a handle; 150. a sterilizing lamp; 200. a placement device; 210. a first placing plate; 220. a first upright rod; 230. a transverse rail; 240. a second placing plate; 250. a second upright stanchion; 260. a longitudinal rail; 270. a fixed mount; 300. an RNA extraction device; 310. erecting a rail; 320. a first slider; 330. a carriage; 340. a second slide carriage; 350. a full-automatic extraction instrument; 400. a detection device; 410. a sequencer; 420. turning the column; 430. a rotating base; 440. a cover plate; 500. a droplet suction device; 510. a base; 520. a connecting seat; 530. a first connecting rod; 540. a first spin column; 550. a second connecting rod; 560. a second spin column; 570. a drip catcher; 600. an analysis device; 610. mounting a plate; 620. an analyzer; 630. a connecting wire; 640. a straight rod; 650. a display screen; 660. a control panel.

Detailed Description

In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the utility model easy to understand, the utility model is further described with the specific embodiments.

Referring to fig. 1-5, the present invention is a pregnant woman premature delivery prediction device based on high-throughput sequencing data, comprising a work box 100, a placement device 200, an RNA extraction device 300, a detection device 400, a drip suction device 500, and an analysis device 600, wherein the work box 100 comprises a box body 110, the box body 110 is of a hollow structure, the placement device 200 comprises a first vertical rod 220, a transverse rail 230, a second vertical rod 250, and a longitudinal rail 260, one end of each of the two transverse rails 230 is connected with the inner lower surface of the box body 110, the other ends of the two transverse rails 230 are respectively in sliding fit with the two first vertical rods 220, one end of each of the two longitudinal rails 260 is connected with the inner lower surface of the box body 110, the other ends of the two longitudinal rails 260 are respectively in sliding fit with the two second vertical rods 250, the RNA extraction device 300 comprises a vertical rail 310 and a first sliding seat 320, one end of each of the two first vertical rails 310 is connected with the inner peripheral surface of the box body 110, the other ends of the two vertical rails 310 are respectively in sliding fit with the two first sliding seats 320, detection device 400 includes sequencer 410, sequencer 410 lower surface and the inside lower surface of box 110 are connected, inhale and drip device 500 and include base 510 and connecting seat 520, base 510 one end and the inside surface connection of box 110, the base 510 other end and two connecting seat 520 upper surface connections, analytical equipment 600 includes mounting panel 610, analysis appearance 620 and connecting wire 630, connecting wire 630 sets up between sequencer 410 and analysis appearance 620, mounting panel 610 all sides and the outside week side connection of box 110.

Referring to fig. 1, the work box 100 further includes a rotation shaft 120, a box cover 130, a handle 140, and a sterilizing lamp 150, wherein one end of the rotation shaft 120 is rotatably engaged with the box body 110, the other end of the rotation shaft 120 is rotatably engaged with the box cover 130, the handle 140 is installed on the peripheral side surface of the box cover 130, the sterilizing lamp 150 is installed on the upper surface inside the box body 110, and the sterilizing lamp 150 can sterilize the inside of the box body 110 in real time.

Referring to fig. 1, the placement device 200 includes a first placement plate 210, a second placement plate 240 and a fixing frame 270, the two fixing frames 270 are respectively installed on one surface of two longitudinal rails 260, the peripheral side surfaces of the two first vertical rods 220 are connected with the peripheral side surface of the first placement plate 210, the two second vertical rods 250 are installed on the peripheral side surface of the second placement plate 240, and the fixing frame 270 can fix the second placement plate 240, so as to facilitate the operation of the drip suction device 500.

Referring to fig. 2, the RNA extraction apparatus 300 further includes a sliding frame 330, a second sliding base 340, and a full-automatic extractor 350, wherein one end of the sliding frame 330 is connected to the peripheral sides of the two first sliding bases 320, the other end of the sliding frame 330 is in sliding fit with the second sliding base 340, the full-automatic extractor 350 is installed on the peripheral side of the second sliding base 340, and the sliding fit between the first sliding base 320 and the vertical rail 310 can change the up-down position of the full-automatic extractor 350.

Referring to fig. 1 and 3, the detecting device 400 further includes a rotating column 420, a rotating base 430 and a cover plate 440, wherein the two rotating bases 430 are both installed on the upper surface of the sequencer 410, the rotating column 420 is respectively matched with the two rotating bases 430 in a rotating manner, the cover plate 440 is matched with the rotating column 420 in a rotating manner, and the sequencing operation is started after the sequencer 410 is tightly covered by the cover plate 440 in a rotating manner.

Referring to fig. 1 and 3, the droplet suction device 500 further includes a first connecting rod 530, a first rotating column 540, a second connecting rod 550, a second rotating column 560 and a droplet suction device 570, wherein one end of the first rotating column 540 is respectively matched with the two connecting seats 520 in a rotating manner, the other end of the first rotating column 540 is matched with the first connecting rod 530 in a rotating manner, the first connecting rod 530 is matched with the second connecting rod 550 in a rotating manner through the second rotating column 560, the droplet suction device 570 is mounted on the lower surface of the second connecting rod 550, and the droplet suction device 570 sucks and drops a sample in a test tube on the second placing plate 240 into the sequencer 410.

Referring to fig. 1, 4 and 5, the analyzer 600 further includes a straight rod 640, a display screen 650 and a control panel 660, the control panel 660 is electrically connected to the analyzer 620 through an electric wire, the analyzer 620 is mounted on the upper surface of the mounting plate 610, the straight rod 640 is disposed between the analyzer 620 and the display screen 650, and the control panel 660 can control the first and second placing plates 210 and 240 to perform commands such as plate-in, plate-out, start, pause and stop, and monitor the degree of the internal task.

The working principle of the utility model is as follows: the disinfection lamp 150 disinfects the interior of the box body 110 in real time, the first upright rod 220 and the transverse rail 230 are in sliding fit to place the collected disposable sampling tube on the first placing plate 210, the second upright rod 250 and the longitudinal rail 260 are in sliding fit to flexibly change the position between the second placing plate 240 and the detection device 400, the sample can be conveniently extracted and transferred by matching the first placing plate and the detection device, wherein the extraction work is completed by the full-automatic extractor 350, the first sliding seat 320 is in sliding fit with the upright rail 310, the second sliding seat 340 is in sliding fit with the sliding frame 330, the position of the full-automatic extractor 350 can be flexibly changed, the full-automatic extractor 350 places the extracted RNA solution in a test tube in the second placing plate 240, then the second upright rod 250 and the longitudinal rail 260 slide to the position of the fixing frame 270 and stop, the RNA solution is absorbed by the drip absorber 570 in the drip absorber 500 and dripped into the sequencer 410, the cover 440 is tightly covered for sequencing work, the timely analysis result of analysis appearance 620 and demonstration are on display screen 650, and control panel 660 can control the first board of placing 210 and second board 240 of placing and advance, and the board goes out, begins, command such as pause, stop to and the control of the interior task degree of carrying on, and clinical feasibility is strong, is equipped with emergency control module and alarm lamp at the outside upper surface of box 110, and the convenience is dealt with under emergency.

The foregoing shows and describes the general principles and broad features of the present invention and advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the utility model as claimed. The scope of the utility model is defined by the appended claims and equivalents thereof.

Claims

1. The utility model provides a pregnant woman premature delivery prediction device based on high-throughput sequencing data, includes work box, placer, RNA extraction element, detection device, inhales and drips device and analytical equipment, its characterized in that: the working box comprises a box body, the box body is of a hollow structure, the placing device comprises a first vertical rod, a transverse rail, a second vertical rod and a longitudinal rail, one end of each transverse rail is connected with the lower surface of the inner part of the box body, the other end of each transverse rail is in sliding fit with the two first vertical rods respectively, one end of each longitudinal rail is connected with the lower surface of the inner part of the box body, the other end of each longitudinal rail is in sliding fit with the two second vertical rods respectively, the RNA extraction device comprises a vertical rail and a first sliding seat, one end of each first vertical rail is connected with the circumferential side surface of the inner part of the box body, the other end of each vertical rail is in sliding fit with the two first sliding seats respectively, the detection device comprises a sequencer, the lower surface of the sequencer is connected with the lower surface of the inner part of the box body, the drip suction device comprises a base and a connecting seat, one end of the base is connected with the upper surface of the inner part of the box body, the base other end and two connecting seat upper surface connections, analytical equipment includes mounting panel, analysis appearance and connecting wire, the connecting wire sets up between sequencer and analysis appearance, mounting panel week side is connected with the outside week side of box.

2. The maternal preterm delivery prediction device based on high-throughput sequencing data of claim 1, wherein: the working box further comprises a rotating shaft, a box cover, a handle and a disinfection lamp, one end of the rotating shaft is in rotating fit with the box body, the other end of the rotating shaft is in rotating fit with the box cover, the handle is installed on the peripheral side face of the box cover, and the disinfection lamp is installed on the upper surface inside the box body.

3. The maternal preterm delivery prediction device based on high-throughput sequencing data of claim 1, wherein: the placing device comprises a first placing plate, a second placing plate and a fixing frame, wherein the fixing frame is respectively installed on one surface of each longitudinal rail, the two side surfaces of the periphery of the first vertical rod are connected with the side surfaces of the first placing plate, and the two side surfaces of the periphery of the second vertical rod are installed on the side surfaces of the periphery of the second placing plate.

4. The maternal preterm delivery prediction device based on high-throughput sequencing data of claim 1, wherein: RNA extraction element still includes balladeur train, second slide and full-automatic extraction appearance, balladeur train one end is connected with two first slide week sides respectively, the balladeur train other end and second slide sliding fit, full-automatic extraction appearance is installed in second slide week side.

5. The maternal preterm delivery prediction device based on high-throughput sequencing data of claim 1, wherein: the detection device further comprises a rotary column, rotary seats and a cover plate, wherein the rotary seats are arranged on the upper surface of the sequencer, the rotary column is in rotary fit with the two rotary seats respectively, and the cover plate is in rotary fit with the rotary column.

6. The maternal preterm delivery prediction device based on high-throughput sequencing data of claim 1, wherein: inhale and drip device still includes head rod, first column spinner, second connecting rod, second column spinner and inhales and drips the ware, first column spinner one end respectively with two connecting seats normal running fit, the first column spinner other end and head rod normal running fit, head rod and second connecting rod pass through second column spinner normal running fit, inhale and drip the ware and install in second connecting rod lower surface.

7. The maternal preterm delivery prediction device based on high-throughput sequencing data of claim 1, wherein: the analysis device further comprises a straight rod, a display screen and a control panel, the control panel is electrically connected with the analyzer through an electric wire, the analyzer is installed on the upper surface of the installation plate, and the straight rod is arranged between the analyzer and the display screen.