CN215684467U

CN215684467U - Kit suitable for adherent cell digestion and cryopreserving

Info

Publication number: CN215684467U
Application number: CN202121692558.2U
Authority: CN
Inventors: 卓鑫杰; 张丽; 姚玲玲; 杨苗; 孟得龙; 宋丹
Original assignee: Huanshenghui Biogenetic Technology Beijing Co ltd
Current assignee: Huanshenghui Biogenetic Technology Beijing Co ltd
Priority date: 2021-07-24
Filing date: 2021-07-24
Publication date: 2022-02-01
Anticipated expiration: 2031-07-24

Abstract

The utility model discloses a kit suitable for adherent cell digestion and cryopreservation, and relates to the technical field of kits, wherein two corresponding micro motors are arranged on the upper surface of the rear wall of a kit shell, the output ends of the two corresponding micro motors are connected with a heat preservation cover, a timer is arranged on the side surface of the kit shell, a control panel is arranged on one side surface of the kit shell on the side edge of the timer, and the number of reagent grooves is increased on a reagent seat, so that the reagent seat can store more reagents and different reagents, thereby preventing experiment failure caused by insufficient reagents, reducing preparation work before operation, reducing labor cost, and timing the opening time of the heat preservation cover by the timer; once the set closing time is reached, the controller directly drives the motor to work, so that the motor drives the heat-insulating cover to reset and close, the reagent is prevented from being exposed in a high-temperature environment for a long time to cause the reagent to lose efficacy, the reagent is protected, the economy and the handiness are reduced, the operation is simple, and manual operation is not needed.

Description

Kit suitable for adherent cell digestion and cryopreserving

Technical Field

The utility model relates to the technical field of kits, in particular to a kit suitable for digestion and cryopreservation of adherent cells.

Background

In recent years, China has been rapidly developed in the fields of stem cells and regenerative medicine, has achieved a plurality of technological developments, and gradually becomes the leading force of the industry. However, the development bases of the method are not independent of primary culture and subculture of cells, a large amount of digestive enzymes and preserving fluid are needed in the process, so far, reagent preparation and preparation in the whole process are complex, the workload is high, if the reagent is prepared in a large amount at one time and preserved for a long time, the effect of influencing the digestion process on cells or inhibiting ice crystals in the cells is not achieved due to overdue cryopreserved reagent, the cells are lost, and the kit usually has a refrigeration effect.

SUMMERY OF THE UTILITY MODEL

Aiming at the defects of the prior art, the utility model provides a kit suitable for adherent cell digestion and cryopreservation, and solves the problem that the reagent fails in an experiment due to insufficient reagent or fails due to the fact that the reagent is in a high-temperature environment for a long time.

In order to achieve the purpose, the utility model is realized by the following technical scheme: a kit suitable for adherent cell digestion and cryopreservation comprises a kit shell, wherein a micro motor is installed on one side edge of the upper end of the kit shell, the output end of the micro motor is connected with a heat preservation cover, a timer is installed on one side surface of the kit shell, and a control panel is installed on one side surface of the kit shell on the side edge of the timer;

the reagent kit comprises a kit shell and is characterized in that a reagent seat is arranged in the kit shell, a plurality of enzyme reagent grooves which are uniformly distributed are formed in the upper surface of the reagent seat, three liquid storage grooves with different sizes are formed in the rear of the enzyme reagent grooves in the upper surface of the reagent seat, and four containing bag grooves with different sizes are formed in one side of the enzyme reagent grooves in the upper surface of the reagent seat.

As a further technical scheme of the utility model, an enzyme agent is arranged in each enzyme agent groove, a preservation liquid agent matched with the enzyme agent groove is arranged in each preservation liquid agent groove, and a placing bag matched with the placing bag groove is arranged in each placing bag groove.

As a further technical scheme of the utility model, the lower part of the reagent seat is provided with an ice bag bin, and a bin opening of the ice bag bin is hinged with a bin door.

As a further technical scheme of the utility model, a first magnetic sheet is embedded in the lower surface of the heat-insulating cover, and a second magnetic sheet corresponding to the first magnetic sheet is embedded in the upper surface of the kit shell.

As a further technical solution of the present invention, the timer is electrically connected to the control panel, and the control panel is electrically connected to the micro motor.

Advantageous effects

The utility model provides a kit suitable for digestion and cryopreservation of adherent cells. Compared with the prior art, the method has the following beneficial effects:

1. the utility model provides a kit suitable for adherent cell digestion and cryopreserving, increases the quantity in reagent groove at the reagent seat to make the reagent seat can store more reagents and different reagents, thereby prevent to cause the experiment failure because the reagent is not enough, reduced the preparation work before the operation, reduce the personnel selection cost.

2. The utility model provides a kit suitable for adherent cell digestion and cryopreserving, time the heat preservation lid open time through the time-recorder, in case reach and set for the closed time after, controller direct drive micro motor work, make it drive the heat preservation lid and reset and close, prevent that reagent from exposing for a long time and leading to reagent inefficacy in high temperature environment, reagent has been protected, it is handy to have reduced the economy, and easy operation, do not need manual operation, timely staff forgets the kit heat preservation lid that finishes, the heat preservation lid also can be automatic closure after the certain time.

Drawings

FIG. 1 is a schematic diagram of a kit suitable for adherent cell digestion and cryopreservation;

FIG. 2 is a schematic diagram of a reagent seat structure of a kit suitable for digestion and cryopreservation of adherent cells;

FIG. 3 is a top view of a reagent seat of a kit suitable for adherent cell digestion and cryopreservation;

FIG. 4 is a side view of a kit suitable for adherent cell digestion and cryopreservation.

In the figure: 1. a cartridge housing; 2. a micro motor; 3. a heat preservation cover; 4. a timer; 5. a control panel; 6. a reagent seat; 7. an enzymatic agent tank; 8. a liquid storage tank; 9. placing a bag groove; 10. an enzymatic agent; 11. preserving the liquid; 12. placing the bag; 13. an ice bag bin; 14. a bin gate; 15. a first magnetic sheet; 16. a second magnetic sheet.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-4, the present invention provides a technical solution of a kit for adherent cell digestion and cryopreservation: a kit suitable for adherent cell digestion and cryopreservation comprises a kit shell 1, wherein a micro motor 2 is installed on one side edge of the upper end of the kit shell 1, an output end of the micro motor 2 is connected with a heat preservation cover 3, a timer 4 is installed on one side surface of the kit shell 1, and a control panel 5 is installed on one side surface of the kit shell 1 on the side edge of the timer 4;

the kit casing 1 internally mounted has reagent seat 6, a plurality of evenly distributed's enzyme agent groove 7 has been seted up to the upper surface of reagent seat 6, three preservation solution groove 8 of different sizes have been seted up at the rear of enzyme agent groove 7 to the upper surface of reagent seat 6, four not equidimension placing bag grooves 9 have been seted up to the upper surface of reagent seat 6 in one side of enzyme agent groove 7, every enzyme agent groove 7 is inside all to be provided with enzyme agent 10, every preservation solution groove 8 is inside all to be provided with the preservation solution 11 rather than the looks adaptation, every placing bag groove 9 is inside to be provided with the placing bag 12 rather than the looks adaptation, when using, enzyme agent groove 7 plays the fixed action to enzyme agent 10, preservation solution groove 8 plays the fixed action to preservation solution 11, placing bag groove 9 plays the fixed action to placing bag 12.

Ice bag storehouse 13 has been seted up to the lower part of reagent seat 6, the door opening in ice bag storehouse 13 articulates there is door 14, when using, open door 14, put into the ice bag to cool down the kit inside toward ice bag storehouse 13, the lower surface of heat preservation lid 3 is embedded to have first magnetic sheet 15, the upper surface of kit casing 1 is embedded to have a second magnetic sheet 16 corresponding with first magnetic sheet 15 position, when using, hold second magnetic sheet 16 through the magnetism of first magnetic sheet 15, be used for fixed heat preservation lid 3.

The timer 4 is electrically connected with the control panel 5, the control panel 5 is electrically connected with the micro motor 2, when the micro motor is used, the timer 4 transmits a signal to the control panel 5, and the micro motor 2 is controlled by the control panel 5 to operate.

The working principle of the utility model is as follows: when the kit is used, the heat-insulating cover 3 is manually opened, then what reagents are needed are taken from the kit, and the timer 4 starts to time while the heat-insulating cover 3 is opened, so that the opening time of the kit can be monitored at any time;

can cool off the kit inside through the ice bag in ice bag storehouse 13, avoid opening because heat preservation lid 3, air conditioning is stored inadequately, lead to the inside temperature of kit slowly to rise and influence reagent storage effect, when the time that heat preservation lid 3 was opened reachd scheduled time, time-recorder 4 can transmit signal for control panel 5, and then control panel 5 can control micro motor 2 work, drive heat preservation lid 3 by micro motor 2 and reset, when heat preservation lid 3 resets, first magnetic sheet 15 on its surface can inhale mutually with second magnetic sheet 16 on the kit casing 1, realize resealing of kit casing 1, the operation is accomplished, it is required to explain, be provided with the battery jar in the bottom of kit casing 1, be used for the storage battery, conveniently be micro motor 2 and time-recorder 4 power supply.

Claims

1. A kit suitable for adherent cell digestion and cryopreservation comprises a kit shell (1) and is characterized in that a micro motor (2) is installed on one side of the upper end of the kit shell (1), the output end of the micro motor (2) is connected with a heat preservation cover (3), a timer (4) is installed on one side of the kit shell (1), and a control panel (5) is installed on one side of the kit shell (1) and on the side of the timer (4);

the kit comprises a kit shell (1), wherein a reagent seat (6) is arranged inside the kit shell (1), a plurality of enzyme agent grooves (7) which are uniformly distributed are formed in the upper surface of the reagent seat (6), three liquid storage agent grooves (8) which are different in size are formed in the rear of the enzyme agent grooves (7) in the upper surface of the reagent seat (6), and four placing bag grooves (9) which are different in size are formed in one side of the enzyme agent grooves (7) in the upper surface of the reagent seat (6).

2. The kit suitable for adherent cell digestion and cryopreservation as claimed in claim 1, wherein an enzyme agent (10) is arranged inside each enzyme agent groove (7), a preservative solution (11) matched with each enzyme agent groove is arranged inside each preservative solution groove (8), and a placing bag (12) matched with each placing bag groove (9) is arranged inside each placing bag groove.

3. The kit suitable for adherent cell digestion and cryopreservation as claimed in claim 1, wherein an ice bag bin (13) is formed at the lower part of the reagent seat (6), and a bin gate (14) is hinged to a bin opening of the ice bag bin (13).

4. The kit for the digestion and cryopreservation of adherent cells as claimed in claim 1, wherein a first magnetic sheet (15) is embedded in the lower surface of the heat preservation cover (3), and a second magnetic sheet (16) corresponding to the first magnetic sheet (15) is embedded in the upper surface of the kit housing (1).

5. The kit for adherent cell digestion and cryopreservation according to claim 1, wherein the timer (4) is electrically connected with the control panel (5), and the control panel (5) is electrically connected with the micro motor (2).