CN215641307U - Film bearing device - Google Patents

Abstract

The utility model provides a membrane bearing device which comprises a bearing plate, wherein the bearing plate is used for fixing and heating/insulating a detection plate, the detection plate is used for protein analysis, a heating groove is arranged on the bearing plate, a placing groove is arranged on the detection plate, the placing groove can contain a membrane and a reagent for protein analysis, the placing groove can be embedded into the heating groove, the bearing plate is made of a good heat conductor material, a plurality of heating grooves are arranged, the heating grooves are linearly arranged at equal intervals, and the detection plate is a plastic-absorbing plate. The bearing plate in the membrane bearing device can bear, heat/keep warm the detection plate, and the standing groove of detection plate can be embedded into the heating groove of bearing plate, makes the bearing plate heat all sides homoenergetic of standing groove, makes the reagent temperature in the detection plate even, and membrane and antigen can better abundant fusion reaction.

Description

Film bearing device

Technical Field

The utility model relates to the technical field of medical instruments, in particular to a membrane bearing device.

Background

Immunoblotting (Western Blot, also known as Western blotting) is a method for detecting a certain protein in a complex sample based on the specific binding of antigen-antibody. The method is a new immune biochemical technology developed on the basis of gel electrophoresis and solid-phase immunoassay. Immunoblotting has become a common technique for protein analysis due to its high resolution of gel electrophoresis and high specificity and sensitivity of solid phase immunoassays. Immunoblotting is most commonly used for detection of protein properties, expression and distribution, such as antibody or antigen detection of viruses, quality determination of polypeptide molecules, and qualitative or semi-quantitative detection of tissue antigens.

At present, western blot related tests are usually carried out on an automatic western blot instrument, and the western blot instrument separates proteins to be tested by adopting different electrophoresis methods according to properties, such as molecular weight, molecular size, electric charge, isoelectric point and the like of the proteins; transferring the proteins in the gel to the polyvinylidene fluoride membrane by current; the principle that the antibody and the antigen are specifically combined is utilized, and the target protein is obtained by taking the antibody as a probe. It is noted that the membrane should be "blocked" by the addition of a non-specific protein, such as bovine serum albumin, prior to the addition of the antibody to prevent non-specific binding of the antibody to the membrane.

A detection plate for placing a membrane and a reagent is arranged in a western blotting instrument in the prior art, and a membrane bearing device in the western blotting instrument only heats the bottom of the detection plate, so that the problem of uneven heating possibly exists; in addition, after the membrane and the reagent are placed in the automatic protein blotting instrument, the membrane and the antigen are subjected to full fusion reaction through the reciprocating swing of a swing mechanism of the instrument, and an accurate detection result is obtained. But traditional rocking mechanism has that required turning moment is big, and rocking mechanism's adaptability is not enough, and the reagent rocks the scheduling problem in the automatic egg white seal mark appearance easily.

SUMMERY OF THE UTILITY MODEL

The present invention is directed to a film carrier device to solve the above problems of the related art.

In order to achieve the above object, the present invention adopts a technical solution in which a membrane carrier device includes a carrier plate for fixing and heating/insulating a detection plate for protein analysis, and a heating bath is provided on the carrier plate.

Furthermore, the detection plate is provided with a placing groove, the placing groove can accommodate a membrane and a reagent for protein analysis, and the placing groove can be embedded into the heating groove.

Further, the carrier plate is made of a good conductor material of heat.

Further, the heating groove is provided with a plurality of, these the heating groove is the straight line equidistance and arranges.

Further, the detection plate is a plastic suction plate.

Further, still include the bottom plate, bottom plate upside position is equipped with the hot plate, the hot plate can be right the loading board heats.

Further, still include the bubble cotton, the bubble cotton is located the hot plate with between the loading board.

Furthermore, a circle of heat preservation cotton is arranged on the bottom plate and surrounds the heating plate.

Further, still include temperature sensor, temperature sensor is used for carrying out temperature monitoring to the loading board.

Further, the bottom plate connects the support plate and the third connecting structure.

In conclusion, the beneficial effects of the utility model are as follows:

the bearing plate in the membrane bearing device can bear, heat/preserve heat for the detection plate, the placing groove of the detection plate can be embedded into the heating groove of the bearing plate, so that the bearing plate can heat all the surfaces of the placing groove, the temperature of the reagent in the detection plate is uniform, and the membrane and the antigen can be subjected to better and sufficient fusion reaction; the swing structure has low requirements on a motor in the rotating mechanism, and compared with the motor in the traditional protein tracing instrument, the motor adopted by the utility model does not need large rotating torque; meanwhile, the utility model does not need to carry out complex control on the motor, such as the accurate control of the rotating speed of the motor, the control of the rotating direction and the like; in addition, the motor adopted by the utility model can select a conventional stepping motor, and the delicate steering change and the rotating speed control of the swing structure can be realized by carrying out simple unidirectional rotation, so that the production cost of the protein tracing instrument can be greatly increased; moreover, the swing structure is provided with multi-gear speed regulation, and can be used for being suitable for various working environments or meeting different working requirements; finally, the sliding bearings made of special engineering plastics are adopted as the revolute pairs in the utility model, and the sliding bearings have a series of characteristics of high wear resistance, dust resistance, dirt resistance, lubrication free, maintenance free and the like, so that technicians do not need to maintain regularly, the maintenance cost is reduced, and the service life of the whole device is greatly prolonged.

Drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings required for the embodiments or the prior art descriptions will be briefly introduced below, and it is obvious that the drawings in the following description are only one embodiment of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.

FIG. 1 is a schematic view of a wobble structure in a western blotter in one direction;

FIG. 2 is a schematic view of a wobble structure in a western blotter in another orientation;

FIG. 3 is an exploded view of a wobble structure in a protein blotter at the location of a pivot point;

FIG. 4 is a schematic view of the wobble structure in a western blotter in another orientation;

FIG. 5 is a schematic structural diagram of a membrane carrying device in the protein blotting apparatus after a detection plate is removed;

FIG. 6 is a schematic view of the internal structure of the membrane carrier;

FIG. 7 is a schematic view of a placement slot on a pick-up plate;

FIG. 8 is a cross-sectional view of a placement slot on an alternative detector board;

FIG. 9 is a graph of the rotational speed imparted to part 1 in a motion simulation;

FIG. 10 is a graph of the rotational speed achieved for part 3 in a motion simulation;

FIG. 11 is a schematic view of a motion simulation with part 3 in a horizontal position;

FIG. 12 is a schematic view of part 3 in a near right extreme condition in a motion simulation;

FIG. 13 is a schematic view of the motion simulation with the part 3 again in a nearly horizontal position;

fig. 14 is a schematic view of the part 3 in a nearly left-side limit state in the motion simulation.

Detailed Description

In order to make the technical problems, technical solutions and advantages of the present invention more apparent, the embodiments of the present invention are described in detail below with reference to the accompanying drawings, it should be noted that the embodiments are only detailed descriptions of the present invention and should not be considered as limitations of the present invention, and all features disclosed in the embodiments of the present invention or all steps in a disclosed method or process can be combined in any way except for mutually exclusive features and/or steps.

The embodiment provides a structure that sways in protein trace appearance for to putting into membrane and the reagent in the automatic protein trace appearance, sway through rocking mechanism's reciprocal and let the abundant fusion reaction of membrane and antigen in the instrument, and then obtain accurate testing result. The swing mechanism comprises a film bearing device 10, a connecting rod mechanism 20 and a rotating mechanism 30, wherein the rotating mechanism 30 is a driving part in the swing mechanism, is generally controlled by a motor and is used for providing kinetic energy for the swing mechanism; the rotating mechanism 30 is connected with the link mechanism 20, and the rotating mechanism 30 can drive the link mechanism 20 to move; the connecting rod mechanism 20 is connected with the membrane bearing device 10, all the steps of detection, liquid adding, incubation, combination, sample adding, cleaning and the like in the western blot instrument are carried out in the membrane bearing device 10, the rotating mechanism 30 can drive the membrane bearing device 10 to move through the connecting rod mechanism 20, the membrane bearing device 10 can swing repeatedly, the reciprocating swing of the membrane bearing device 10 can enable a sample and a reagent to be fully fused and reacted, and the aim of accurate detection is further fulfilled.

Referring to fig. 1, the membrane carrier 10 has a pivot 11 about which the membrane carrier 10 can pivot, and the pivot 11 is generally located on a vertical plane (a perpendicular bisector in the drawing, and a vertical plane in three dimensions) of the membrane carrier 10 in order to uniformly fuse the membrane and the antigen in the apparatus. The pivot 11 is also located on the fixing plate 12, the membrane carrier 10 is connected with the fixing plate 12 in a pivot fit manner through the pivot 11, and in order to ensure the installation stability and the rotation stability of the membrane carrier 10 on the fixing plate 12, two pivots 11 are provided, as shown in fig. 2. Specifically, be equipped with fixing base 121 on the fixed plate 12, be equipped with two on the fixing base 121 and support ear 122, all be equipped with through-hole 123 in two support ears 122, through-hole 123 is used for installing round pin 124, it is equipped with backup pad 125 still to overlap on the round pin 124, backup pad 125 connect in membrane load device 10. The pin 124 is the rotation pivot 11 of the film carrier 10 and the fixing seat 121, and is also the rotation center of the two.

Referring to fig. 1, the link mechanism 20 includes a first connecting structure 21, a second connecting structure 22 is connected to the first connecting structure 21 in a rotating fit manner, and a third connecting structure 24 is connected to the second connecting structure 22 in a rotating fit manner, wherein the first connecting structure 21 is connected to the rotating mechanism 30, and the third connecting structure 24 is connected to the film carrier 10. The first connecting structure 21, the second connecting structure 22 and the third connecting structure 24 may be three connecting rods, adjacent connecting rods are connected in a rotation fit manner, and the simplest rotation fit connecting manner is to insert into rotation fit positions of the adjacent connecting rods through a rotation shaft. In this embodiment, since the first connecting structure 21 is connected to the motor in the rotating mechanism 30, if the first connecting structure 21 is a connecting rod, when the motor is in operation, the rotating shaft of the motor may receive extremely uneven load due to the gravity and centrifugal force of the first connecting structure 21, which may affect the service life of the motor, as a preferred technical solution, the first connecting structure 21 is disc-shaped, so that the gravity is distributed as uniformly as possible, which may reduce the uneven load received by the rotating shaft of the motor, and the disc-shaped first connecting structure 21 and the second connecting structure 22 rotatably and fittingly connected thereto form a structure similar to an eccentric wheel.

Preferably, the film carrier 10 is generally rectangular, and in order to make the film carrier 10 uniformly stressed when the motor in the rotating mechanism 30 is started, the third connecting structure 24 is located on a middle vertical plane of the film carrier 10 along the long side direction, as shown in fig. 2. It should be noted that the third connecting structure 24 is disposed eccentrically, as shown in fig. 1, that is, the third connecting structure 24 cannot be located on the connecting line between the two rotation fulcrums 11, because the rotating mechanism 30 cannot drive the film carrier 10 to perform a swinging motion when the third connecting structure 24 is located on the connecting line between the two rotation fulcrums 11. Further, the greater the distance from the third connecting structure 24 to the pivot point 11, i.e., the greater the eccentric distance, the smaller the maximum swing amplitude of the film carrier 10, all other things being equal.

In the automatic egg white imprinting appearance of conventionality, the servo motor that uses, its servo motor's output shaft and the rotation fulcrum 11 that the membrane bore manufacturing 10 are connected, in order to realize the action of swaing, need to input forward pulse, reverse pulse signal to servo motor, very loaded down with trivial details, servo motor's high price has improved the cost of egg white imprinting appearance greatly simultaneously. In the present invention, under the action of the link mechanism 20, the motor in the rotating mechanism 30 only needs to rotate in one direction to control the film carrier 10 to perform a swing operation, and there is no need to repeatedly input a forward pulse and a reverse pulse signal, which is much more convenient; meanwhile, after repeated forward pulse and reverse pulse signals are not required to be input, the rotating precision of the motor during working is not required, the servo motor can be replaced by a common stepping motor, the cost of the stepping motor is far lower than that of the servo motor, and the manufacturing cost of the automatic protein tracing machine is obviously reduced.

Preferably, for the convenience of counting the number of times of swinging that the inner membrane of the device bears and makes 10, be equipped with an opto-coupler separation blade 14 on link mechanism 20, correspond to it, still be equipped with an opto-coupler induction structure 15 in the device, along with slewing mechanism 30 starts the drive link mechanism 20 moves, every round of motor in slewing mechanism 30, on link mechanism 20 opto-coupler separation blade 14 just once (shelter from once) opto-coupler induction structure 15 carries out a count. Further, as shown in fig. 1, the optical coupling blocking piece 14 is installed on the second connecting structure 22, when the film carrying device 10 is located at a horizontal position, the right end of the optical coupling blocking piece 14 just covers the optical coupling inductor 15, and the optical coupling inductor 15 is located on a necessary path in the movement process of the optical coupling blocking piece 14.

Preferably, the motor in the rotating mechanism 30 is kept stationary to ensure that the parameters of amplitude, frequency, angle, etc. of each oscillation of the film carrier manufacturing 10 are kept consistent. In this embodiment, the fixed plate 12 is stationary, so the rotating mechanism 30 can be mounted on the fixed plate 12. Specifically, install first mounting panel 16 on fixed plate 12, first mounting panel 16 is L shape, motor 17 can be installed to first mounting panel 16, the output shaft of motor 17 first connection structure 21. And a second mounting plate 18 is arranged on the first mounting plate 16, and the second mounting plate 18 is used for mounting the optical coupling induction structure 15.

Preferably, the rotating mechanism 30 is located at a lower side position of the third connecting structure 24 when the film carrier manufacturing 10 is in a horizontal state. Further, the rotating shaft of the motor 17 in the rotating mechanism 30 is located at a position right below the third connecting structure 24, that is, a connecting line between the third connecting structure 24 and the rotating shaft of the motor 17 is in a vertical state.

Preferably, the present invention has at least four rotation pairs, which are a rotation pair of a motor in the rotation mechanism 30, a rotation pair between the first connection structure 21 and the second connection structure 22, a rotation pair between the second connection structure 22 and the third connection structure 24, and a rotation pair at the position of the rotation fulcrum 11. These revolute pairs all require high frequency rotation during operation of the western blotting apparatus, for example, at the position of the fulcrum 11, the membrane carrier 10 is repeatedly swung around the fulcrum 11, in order to improve the smoothness of rotation, sliding elements 13 are provided between the pin 124 and the two support lugs 122, and between the pin 124 and the support plate 125, most commonly the sliding elements 13 are rolling bearings, it has the advantages of low cost, stable operation, and the like, but also has the problem of frequent need of lubricating oil or lubricating grease, is more complicated, if the lubricating oil or the lubricating grease is not added in time, the bearing materials can be peeled off, the bearing flanges can be scratched, resulting in overheating of the rollers, extreme localized heating that can generate metal flow in the bearing, altering the original material and geometry of the bearing, eventually leading to excessive roller tilting, cage damage and complete bearing lock-up. Therefore, in these environments of high-frequency rotation, if a rolling bearing is selected, a technician is required to check and maintain the rolling bearing regularly, which is cumbersome.

In the present embodiment, the sliding member 13 is a sliding bearing (press-fit bearing) made of a special engineering plastic, and the thermoplastic base plastic material meeting the requirement is screened, and the material is usually added with reinforcing fibers to enhance the compressive strength, and is also added with solid grease for optimizing the wear resistance. These solid lubricating particles "embedded" in the matrix material are of paramount importance for the requirements of "dry running". In operation, sliding bearings typically release thousands of solid lubricant particles stored in a matrix material, due to pressure and motion, onto the contact surfaces of the shaft and bearing, sufficient to provide sufficient solid lubrication of the contact surfaces to achieve dry running. The sliding bearing made of engineering plastics has a series of characteristics of high wear resistance, dust resistance, dirt resistance, lubrication free, maintenance free and the like, is particularly suitable for the device, technical personnel do not need to maintain regularly, maintenance cost is reduced, and the service life of the whole device is greatly prolonged. In particular, the plain bearing of iglidur, germany, can be used. Here, the rotation pair at the position of the rotation fulcrum 11 is taken as an example, and the rotation pairs at other positions can adopt the similar structure.

In the automatic egg white mark appearance among the prior art the operation that sways that membrane load device 10 carried out is controlled by servo motor input forward pulse, reverse pulse signal, works as when membrane load device 10 moves to extreme position to one side, need switch over at once and input a reverse pulse signal and make it move to the opposite side, avoid membrane load device 10 excessively moves to one side, the condition that the reagent spills out appears. Then, when the two pulse signals are switched, there is a moment of sudden change of the signals, at this moment, the membrane carrier 10 suddenly moves in the reverse direction, but the reagent in the membrane carrier 10 has inertia to move in the original direction, the opposite impact of the two is very easy to cause the reagent in the membrane carrier 10 to splash, in order to solve this problem, a technician usually starts to reduce the input pulse signal when the membrane carrier 10 moves to a limit position quickly, so that the sudden change process is as gentle as possible, and after the membrane carrier 10 passes the limit position, starts to amplify a reverse pulse signal, so that the whole control process of accelerating the membrane carrier 10 to reversely swing … … is very tedious, and the manufacturing cost of the device is increased again.

In order to highlight the advantages of the rocking mechanism of the present invention, motion simulation is performed on several core components in the rocking mechanism, referring to fig. 9-14, component 1 corresponds to the first connecting structure 21, component 2 corresponds to the second connecting structure 22, component 3 corresponds to the membrane carrier 10, component 4 corresponds to the fixing plate 12, arrow 5 corresponds to the speed (vector) of the edge position of the membrane carrier 10, the length of arrow 5 corresponds to the speed, and the direction of arrow 5 corresponds to the speed direction.

Now, the part 1 is endowed with an angular speed of 15r/min, namely 15 turns per minute, and the figure 9 is a power (angular speed) input curve of the part 1; fig. 10 is a graph of the power (angular velocity) output of the part 4 about its fulcrum.

Referring to fig. 11, the state is the moving speed of the edge of the part 3 (arrow 5 position, the same below) when the part is in the horizontal state; referring to fig. 12, the state is the moving speed of the edge of the part 3 at the nearly right limit state after 1 second from the state of fig. 11; referring to fig. 13, the state is the state of fig. 12, after 1 second, the moving speed of the edge of the part 3 is nearly horizontal; referring to fig. 14, the state is the moving speed of the edge of the part 3 at the nearly left limit state after 1 second from the state of fig. 13.

As can be clearly seen in conjunction with fig. 10 and the schematic views of fig. 11-14 in various states: when the component 3 is in a horizontal state, referring to fig. 11 and 13, the reagent in the component 3 is not easy to leak because the component 3 is placed stably, and at this time, a larger rotation speed is expected to be given to the component 3, which is helpful for allowing the membrane in the component 3 to perform a sufficient fusion reaction with the antigen, whereas in the present invention, the arrow 5 corresponding to fig. 11 and 13 is longer, the corresponding instantaneous speed in fig. 10 is close to the maximum value of the rotation speed, and the swing mechanism of the present invention can just give a larger movement speed to the component 3 when the component 3 is in the horizontal state, namely, give a larger angular speed to the rotation fulcrum position (the rotation fulcrum 11 of the membrane bearing device 10) of the component 3; when the component 3 is in the left limit state or the right limit state, referring to fig. 12 and 14, the reagent in the component is easy to leak because the component 3 is in the inclined state, and in the two states, the component 3 immediately swings reversely, which promotes the reagent to leak, so that in order to avoid the leakage, a small rotating speed is expected to be given to the component 3, so that the conversion process is as gentle as possible, whereas in the utility model, the arrow 5 corresponding to fig. 12 and 14 is short, and the corresponding instantaneous rotating speed in fig. 10 is close to 0, so that the speed reduction near the limit position is perfectly realized, and the required speed of the utility model can be well realized.

Through the swing structure of the utility model, the higher requirement on the motor in the rotating mechanism 30 is not required any more, and meanwhile, according to the lever principle, because the rotating mechanism 30 drives the third connecting structure 24, and the third connecting structure 24 is arranged eccentrically, compared with the traditional swing mechanism motor which is directly output to the rotating fulcrum 11, the motor in the utility model does not need a large rotating moment; in addition, the utility model does not need to carry out complex control on the motor, such as the control on the rotating speed and the rotating direction of the motor at each moment, and only needs to select the conventional stepping motor under a delicate mechanical structure, thereby being capable of greatly reducing the production cost of the protein track plotter. Preferably, the swing structure provided by the utility model is provided with multi-gear speed regulation, and is suitable for various working environments or different working requirements.

The membrane bearing device 10 comprises a heating device 51 and a detection plate 52, wherein the detection plate 52 is used for placing a membrane and adding a reagent, and the reagent is required to submerge the membrane, so that the membrane and an antigen are subjected to sufficient fusion reaction, and an accurate detection result is obtained. The membrane is fixed on a membrane strip 54, and the detection plate 52 is provided with a plurality of placement grooves 53 (a plurality represents 3 or more than 3, and the same applies throughout), so that the western blotting apparatus can simultaneously perform a plurality of tests, and in the embodiment, the placement grooves 53 are provided with 36. The film strips 54 are placed in the placing groove 53, the width of the placing groove 53 is matched with the width of the film strips 54 (the width of the placing groove 53 is equal to the width of the film strips 54 or the width of the placing groove 53 is slightly larger than the width of the film strips 54 and is 0-2mm larger), the length of the placing groove 53 is larger than the length of the film strips 54, a protruding bulge 55 is arranged in the placing groove 53, and the placing groove 53 is divided into two parts by the bulge 55: the film carrying device comprises a mounting part 56 and a liquid transferring part 57, wherein the mounting part 56 is used for placing the film strip 54, and the length of the mounting part is matched with that of the film strip 54 (the length of the placing groove 53 is equal to that of the film strip 54 or the length of the placing groove 53 is slightly larger than that of the film strip 54 and is 0-2mm larger), so that the film strip 54 is limited in the placing groove 53 and cannot move during the operation of the film carrying device 10. The standing groove size in traditional western blot appearance matches with membrane strip size just, after the detection finishes, the interior waste liquid device of western blot appearance needs to take away the reagent in the standing groove, and the waste liquid device is for the complete reagent of arranging in the standing groove as far as possible, the pipe in the waste liquid device needs to contact with the bottom of standing groove, and the bottom of standing groove is placed and is placed the membrane strip, inevitable then can let the pipe of waste liquid device contact with the membrane strip, and the pipe of waste liquid device needs to absorb the reagent in all standing grooves, the reagent in the different standing grooves is different probably, consequently, when the pipe of waste liquid device contacts with the membrane strip probably because the pipe glues reagent in other standing grooves then the pollution to the membrane strip, lead to the inaccurate membrane strip test result. In the present invention, the placement groove 53 is divided into two parts, i.e., the mounting part 56 and the liquid transferring part 57, the mounting part 56 is used for limiting the movement of the membrane strip 54, the liquid transferring part 57 is used for inserting the tube of the waste liquid device, and the tube of the waste liquid device does not contact the membrane strip 54, so that the test result of the membrane strip 54 is not affected.

Preferably, the detection plate 52 is provided with a plurality of the placement grooves 53, the placement grooves 53 are arranged along a straight line, and the distance between two adjacent placement grooves 53 is equal, so that the movement of the waste liquid device is conveniently controlled to discharge waste liquid from each placement groove 53. In addition, the detection board 52 can be taken down from the heating device 51, and in order to ensure the cleanness of the detection board 52 and reduce the production cost of the detection board 52, the detection board 52 is a plastic suction board, and the detection board 52 is a disposable consumable material.

Preferably, two projections 55 are provided in one of the placement grooves 53, and the two projections 55 are symmetrically arranged, but a certain gap is required between the two projections 55 to allow the liquid in the mounting portion 56 and the liquid transfer portion 57 to flow, and the projections 55 do not interfere with the waste liquid device to draw all the reagents in the placement groove 53 from the liquid transfer portion 57.

Preferably, the depth of the liquid transfer section 57 in the placement tank 53 is deeper than the depth of the mounting section 56, and when the liquid transfer section 57 performs liquid discharge, the reagent in the mounting section 56 is promoted to flow to the liquid transfer section 57, thereby contributing to the clean discharge of the reagent in the placement tank 53.

Preferably, referring to fig. 7, the upper opening of the placing groove 53 and the bottom of the placing groove 53 are the same in size, so as to facilitate the insertion and removal of the film strip 54. In some other embodiments, referring to fig. 8, the upper opening of the placement groove 53 is smaller than the bottom of the placement groove 53, in this case, when the film strip 54 is placed, the film strip 54 needs to be slightly inserted into the placement groove 53 in an inclined manner, the film strip 54 falling into the placement groove 53 is stably placed at the bottom of the placement groove 53 under the action of gravity, when the film strip 54 needs to be taken out, a tweezers can be used to clamp one end of the film strip 54 in the placement groove 53 and take out the film strip, or a pulling object is fixed on the film strip 54, and when the film strip 54 is located in the placement groove 53, the pulling object is also exposed out of the placement groove 53, so that a tester only needs to slightly pull the pulling object when taking out the film strip 54. Such a structure of the placement tank 53 having a small upper opening and a large bottom area contributes to a reduction in the amount of reagent to be added into the placement tank 53, and the reagent in the placement tank 53 easily flows over the membrane strip 54.

Preferably, the heating device 51 comprises a carrier plate, and the carrier plate has two functions, one is used for carrying the detection plate 52, and the other is used for heating/insulating the detection plate 52 and the reagent therein. Therefore, the material of the carrier plate is a good thermal conductor, such as solid metal: iron, aluminum, stainless steel, and the like. In order to make the temperature of the reagent in the detection plate 52 uniform and make the membrane and the antigen perform better and sufficient fusion reaction, the bearing plate is provided with heating grooves 61 which are matched with the placing grooves 53, and because the placing grooves 53 on the detection plate 52 are linearly arranged along the linear direction at equal intervals, the heating grooves 61 on the bearing plate are also linearly arranged at equal intervals. When the detection plate 52 is placed on the bearing plate, the placing groove 53 of the detection plate 52 is embedded into the heating groove 61 of the bearing plate, such a design enables the bearing plate to heat all the surfaces of the placing groove 53 (two side surfaces in the extension direction and one bottom surface, and two side surfaces in the extension direction are too small and can be ignored), whereas the western blotting instrument in the prior art only contacts with the bottom surface of the detection plate 52, so that only the bottom surface of the detection plate 52 can be heated, and relatively speaking, the heating/heat preservation effect of the present invention is better.

Referring to fig. 6, the heating device 51 further includes a bottom plate 62, foam 63, a heating plate 64, heat-insulating foam 65, and a temperature sensor 66, the bottom plate 62 is connected to the supporting plate 125 and the third connecting structure 24, the foam 63 is located on the bottom plate 62, the heating plate 64 is located on the foam 63, and the heating plate 64 is in contact with the supporting plate and can transfer heat to the supporting plate. In order to achieve a better heat preservation effect, a circle of heat preservation cotton 65 is enclosed on the bottom plate 62, and the temperature sensor 66 in the heating device 51 is used for monitoring the temperature of the heating device 51.

The above description is only the specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto, and any changes or substitutions that are not thought of through creative work should be covered within the protection scope of the present invention, and therefore, the protection scope of the present invention should be subject to the protection scope defined by the claims.

Claims (9)

1. The membrane bearing device is characterized by comprising a bearing plate, wherein the bearing plate is used for fixing and heating/insulating a detection plate, the detection plate is used for protein analysis, and a heating groove is arranged on the bearing plate;

the detection plate is provided with a placing groove, the placing groove can accommodate a membrane and a reagent for protein analysis, and the placing groove can be embedded into the heating groove.

2. The film carrier of claim 1, wherein said carrier plate is made of a good conductor of heat material.

3. The film carrier device according to claim 1, wherein the heating grooves are provided in plural, and the heating grooves are arranged in a straight line at equal intervals.

4. The film carrier of claim 1, wherein the detection panel is a blister panel.

5. The film carrier of claim 1, further comprising a bottom plate, wherein a heating plate is disposed on an upper side of the bottom plate, and the heating plate can heat the carrier plate.

6. The film carrier of claim 5, further comprising foam located between the heating plate and the carrier plate.

7. The film carrier of claim 5, wherein a ring of insulation cotton is provided on said base plate, said insulation cotton surrounding said heating plate.

8. The membrane carrier device of claim 5, further comprising a temperature sensor for temperature monitoring of the carrier plate.

9. The film carrier of claim 5, wherein the base plate connects the support plate and the third connecting structure.

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