CN215628016U - NK cell culture device - Google Patents
NK cell culture device Download PDFInfo
- Publication number
- CN215628016U CN215628016U CN202121171164.2U CN202121171164U CN215628016U CN 215628016 U CN215628016 U CN 215628016U CN 202121171164 U CN202121171164 U CN 202121171164U CN 215628016 U CN215628016 U CN 215628016U
- Authority
- CN
- China
- Prior art keywords
- box body
- bearing box
- cell culture
- hook
- culture
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- 238000004113 cell culture Methods 0.000 title claims abstract description 46
- 210000000822 natural killer cell Anatomy 0.000 title claims abstract description 35
- 238000012258 culturing Methods 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 3
- 239000007788 liquid Substances 0.000 abstract description 13
- 210000004027 cell Anatomy 0.000 abstract description 7
- 238000002474 experimental method Methods 0.000 description 4
- 239000000945 filler Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000009423 ventilation Methods 0.000 description 4
- 238000003306 harvesting Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses an NK cell culture device which comprises a bearing box body, wherein air holes are uniformly distributed in the side surface of the bearing box body, an opening structure is arranged in the side surface of the bearing box body, a hook used for hanging a cell culture bag is arranged on the inner side of the bearing box body, and a culture bag liquid filling opening clamping ring used for fixing a liquid filling opening of the cell culture bag is arranged on one side surface of the bearing box body. The utility model can ensure that cells are cultured in a flat way or in a standing way, and meanwhile, when the cells are cultured in the flat way, the splicing culture of a plurality of culture devices can be realized by virtue of the pre-designed grooves, so that the culture space is greatly saved, and the culture efficiency is improved.
Description
Technical Field
The utility model relates to the technical field of NK cell culture, in particular to an NK cell culture device.
Background
In the current NK cell culture, cells are filled in an NK cell culture bag and then are laid on a partition plate of a cell culture box, and a final culture system can reach about 2L after liquid supplement.
However, when the filling or harvesting operation is performed, the cell culture bag is directly taken out of the incubator, and an auxiliary hook is required to suspend the cell culture bag, and then the operation is performed, so that the convenience of the operation is low.
Therefore, an NK cell culture apparatus is proposed.
SUMMERY OF THE UTILITY MODEL
The present invention is directed to provide an NK cell culturing apparatus to solve the above problems of the background art.
In order to achieve the purpose, the utility model provides the following technical scheme: the utility model provides a NK cell culture device, is including bearing the box body, the side evenly distributed who bears the box body has the bleeder vent, the side of bearing the box body is equipped with open structure, the inboard of bearing the box body is equipped with and is used for carrying out the couple that hangs to the cell culture bag, a side of bearing the box body is equipped with and is used for carrying out the culture bag filling opening snap ring fixed to the filling opening of cell culture bag.
Preferably, the hook is connected with the bearing box body in a sliding mode, a sliding groove is fixedly formed in the surface of the bearing box body, a sliding block is connected in the sliding groove in a sliding mode, and the sliding block is fixedly connected with the hook.
Preferably, the hook is connected with the bearing box body in a sliding mode, a sliding groove is fixedly formed in the surface of the bearing box body, a sliding block is connected in the sliding groove in a sliding mode, and the sliding block is hinged to the hook.
Preferably, the hook is fixedly connected with the bearing box body.
Preferably, the hook is hinged with the bearing box body, and the hook rotates in the horizontal direction.
Preferably, the bottom of the bearing box body is rotatably connected with a rotary shifting piece.
Preferably, an edge groove is formed in one side face of the bearing box body, an edge protrusion is formed in one side face of the edge convex groove of the bearing box body, and the edge groove corresponds to the edge protrusion.
Preferably, the air holes are round air holes.
Preferably, the bearing box body is made of plastic materials.
Preferably, the bearing box body is a rectangular box body.
Compared with the prior art, the utility model has the beneficial effects that:
1. the utility model not only realizes the diversification of the NK cell culture process, namely the cell can be cultured in a flat-laying way or in a standing way, but also can realize the splicing culture of a plurality of culture devices by virtue of the edge groove designed in advance during the flat-laying culture, thereby greatly saving the culture space and improving the culture efficiency.
2. The device can be used as an NK cell culture device and an auxiliary device in the operation process of NK cells, is made of plastic, can be sterilized by high-temperature damp heat, and can enter a workbench to perform experiments along with a sample after surface sterilization, so that cells can be well fixed in the experiment process, direct contact between personnel and the cell culture device is reduced, and disturbance to the cell culture environment is small.
Drawings
FIG. 1 is an isometric view of an embodiment of the utility model;
FIG. 2 is a schematic front view of an embodiment of the present invention;
FIG. 3 is a schematic view of a side surface of a box body provided with a hook in a sliding manner according to an embodiment of the present invention;
FIG. 4 is an isometric view of a load bearing cassette having edge notches according to an embodiment of the present invention;
FIG. 5 is a schematic front view of a loading cassette with an edge groove according to an embodiment of the present invention;
fig. 6 is an isometric view of a rotary paddle in an embodiment of the utility model.
In the figure: 1. a bearing box body; 2. air holes are formed; 3. an edge groove; 4. a liquid filling port snap ring; 5. a chute; 6. a slider; 7. the edge is raised; 8. rotating the shifting sheet; 9. and (4) hanging hooks.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like, indicate orientations and positional relationships based on those shown in the drawings, and are used only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be considered as limiting the present invention.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, features defined as "first", "second", may explicitly or implicitly include one or more of the described features. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise. Furthermore, the terms "mounted," "connected," and "connected" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Referring to fig. 1 to 6, the present invention provides a technical solution of an NK cell culture apparatus:
as shown in figures 1 and 2, an NK cell culture device comprises a bearing box body 1, wherein air holes 2 are uniformly distributed in the side surface of the bearing box body 1, an opening structure is arranged in the side surface of the bearing box body 1, a hook 9 used for hanging a cell culture bag is arranged on the inner side of the bearing box body 1, and a culture bag liquid filling opening clamping ring 4 used for fixing a liquid filling opening of the cell culture bag is arranged on one side surface of the bearing box body 1. Exemplarily, bear box body 1 and be the rectangle box body, bear box body 1 length width height and be 35cm x 20cm x 6cm, bleeder vent 2 is circular bleeder vent 2, bear box body 1 and be the plastic material.
During the in-service use, can put into the cell culture bag through open structure and bear in the box body 1 to liquid filler of cell culture bag is fixed through culture bag liquid filler snap ring 4. The ventilation efficiency is improved through the arrangement of the ventilation holes 2.
Illustratively, as shown in fig. 2, the hook 9 can be hinged or fixedly connected with the carrying case 1.
When the hook 9 is hinged with the bearing box body 1, the hook 9 can horizontally rotate.
Illustratively, as shown in fig. 3, the hook 9 is slidably connected to the carrying case 1, a sliding groove 5 is fixedly disposed on a side surface of the carrying case 1, a sliding block 6 is slidably connected to the sliding groove 5, and the sliding block 6 is fixedly connected or slidably connected to the hook 9. When the hook 9 is rotatably connected with the sliding block 6, the hook 9 can horizontally rotate 180 degrees around the sliding block 6.
Through setting up spout 5 and slider 6, the position of slidable regulation couple 9 to this satisfies the in-service use demand. And, to the condition that couple 9 and slider 6 rotate to be connected, can fold to accomodate couple 9 to this makes things convenient for actual use, places NK cell culture device especially.
As an embodiment of the present invention, as shown in fig. 4 and 5, the present invention further includes that a side surface of the carrying box body 1 is provided with an edge groove 3, a side surface of the edge groove of the carrying box body 1 is provided with an edge protrusion 7, and the edge groove 3 corresponds to the edge protrusion 7.
Through the setting of edge recess 3 and edge protrusion 7, can make things convenient for adjacent bearing box body 1 between splice, realize the concatenation cultivation to NK cell culture apparatus to this culture space has been saved greatly, has promoted cultivation efficiency.
As an embodiment of the present invention, as shown in fig. 6, the present invention further includes that the bottom of the bearing box 1 is rotatably connected with two groups of rotating shifting pieces 8, and the rotating shifting pieces 8 can rotate 180 °. After the rotary stirring piece 8 is rotated and unfolded, the whole NK cell culture device can be stably placed.
In actual use, the culture bag liquid filling opening is fixed through the liquid filling opening clamping ring 4. Can put into the cell culture bag through open structure and bear in the box body 1 to liquid filler of cell culture bag is fixed through culture bag liquid filler snap ring 4. The ventilation efficiency is improved through the arrangement of the ventilation holes 2. And taking the NK cell culture device out of the incubator, and then adding liquid or harvesting. When placing NK cell culture apparatus and cultivateing in the incubator, splice between the adjacent NK cell culture apparatus to save culture space. Open rotatory plectrum 8 through rotating, can make things convenient for NK cell culture device to stand.
The utility model not only realizes the diversification of the NK cell culture process, namely the cell can be cultured in a flat way or in a standing way, but also can realize the splicing culture of a plurality of culture devices by virtue of the pre-designed groove during the flat culture, thereby greatly saving the culture space and improving the culture efficiency.
The device can be used as an NK cell culture device and an auxiliary device in the operation process of NK cells, is made of plastic, can be sterilized by high-temperature damp heat, and can enter a workbench to perform experiments along with a sample after surface sterilization, so that cells can be well fixed in the experiment process, direct contact between personnel and a cell culture bag is reduced, and disturbance to the cell culture environment is small.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.
Claims (10)
1. An NK cell culture apparatus characterized in that: including bearing box body (1), the side of bearing box body (1) is equipped with open structure, the inboard of bearing box body (1) is equipped with and is used for carrying out couple (9) that hang to the cell culture bag, a side of bearing box body (1) is equipped with and is used for carrying out culture bag filling opening snap ring (4) fixed to the filling opening of cell culture bag.
2. The NK cell culturing device according to claim 1, wherein: couple (9) with bear box body (1) sliding connection, bear box body (1) fixed surface and be provided with spout (5), sliding connection has slider (6) in spout (5), slider (6) with fixed connection between couple (9).
3. The NK cell culturing device according to claim 1, wherein: couple (9) with bear box body (1) sliding connection, it is provided with spout (5) to bear box body (1) fixed surface, sliding connection has slider (6) in spout (5), slider (6) with it is articulated between couple (9).
4. The NK cell culturing device according to claim 1, wherein: the hook (9) is fixedly connected with the bearing box body (1).
5. The NK cell culturing device according to claim 1, wherein: the hook (9) is hinged with the bearing box body (1), and the hook (9) rotates in the horizontal direction.
6. The NK cell culturing device according to any one of claims 2 to 5, wherein: the bottom of the bearing box body (1) is rotatably connected with a rotary shifting piece (8).
7. An NK cell culture apparatus according to any one of claims 2 to 5: one side of the bearing box body (1) is provided with an edge groove (3), one side of the edge convex groove of the bearing box body (1) is provided with an edge bulge (7), and the edge groove (3) corresponds to the edge bulge (7).
8. An NK cell culture apparatus according to any one of claims 2 to 5: the side surface of the bearing box body (1) is uniformly distributed with air holes (2), and the air holes (2) are circular air holes (2).
9. An NK cell culture apparatus according to any one of claims 2 to 5: the bearing box body (1) is made of plastic materials.
10. An NK cell culture apparatus according to any one of claims 2 to 5: the bearing box body (1) is a rectangular box body.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202121171164.2U CN215628016U (en) | 2021-05-27 | 2021-05-27 | NK cell culture device |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202121171164.2U CN215628016U (en) | 2021-05-27 | 2021-05-27 | NK cell culture device |
Publications (1)
Publication Number | Publication Date |
---|---|
CN215628016U true CN215628016U (en) | 2022-01-25 |
Family
ID=79939868
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202121171164.2U Expired - Fee Related CN215628016U (en) | 2021-05-27 | 2021-05-27 | NK cell culture device |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN215628016U (en) |
-
2021
- 2021-05-27 CN CN202121171164.2U patent/CN215628016U/en not_active Expired - Fee Related
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
GR01 | Patent grant | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20220125 |