CN114231412A - Special magazine of cell culture board - Google Patents
Special magazine of cell culture board Download PDFInfo
- Publication number
- CN114231412A CN114231412A CN202111484701.3A CN202111484701A CN114231412A CN 114231412 A CN114231412 A CN 114231412A CN 202111484701 A CN202111484701 A CN 202111484701A CN 114231412 A CN114231412 A CN 114231412A
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- Prior art keywords
- base
- plate
- cell culture
- cassette
- water
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a special cassette for a cell culture plate, which comprises a base and a box cover, wherein the base is provided with a groove for placing the cell culture plate, a detachable water-stop plate is arranged in the groove, the top of the groove is provided with an inner side structural plate protruding from the base, the box cover is provided with a label area, and the base, the box cover and the inner side structural plate are all made of light-proof plastic materials. According to the size of a cell culture plate commonly used in the market, a square light-proof box with a proper size is specially made for placing the cell culture plate therein, so that light-proof culture and incubation are realized; the light-shielding box can be repeatedly used, so that the cost is reduced; the surface is provided with a label position, so that the light-shading plate can be conveniently marked; the bottom is provided with a groove, the plate is covered with a bulge, so that the plate is convenient to vertically stack, the stability is realized, and the space is saved.
Description
Technical Field
The invention belongs to the technical field of cell culture, and particularly relates to a cassette special for a cell culture plate.
Background
Currently, cell culture plates have specifications of 6 wells, 12 wells, 24 wells, 48 wells and the like. The size of the culture plate is uniform, and the culture plate is generally 12.7cm long, 8.5cm wide and 2.2cm thick.
Biological assay often needs the operation of keeping out of the sun, need keep out of the sun to incubate usually after adding sensitization reagent in toward the cell culture board, consequently need keep out of the sun box and deposit the cell culture board, will realize the light-resistant to the cell culture board simultaneously.
At present, cell culture plates are sold in the market by using light-shielding boxes, and the light is shielded by adopting wrapping modes such as tinfoil paper, black plastic bags and the like, so that certain defects exist in the use process, the use effect is poor, most of the boxes are designed for placing slices, the size is large, the occupied space is large, and the size is completely different from that of the culture plates; the height in the box is not enough, and after the culture plate is placed, the box cover of the light-shading box is difficult to close, so that the omnibearing light shading cannot be realized. In addition, although aluminum foil, tinfoil and black plastic bags can be used for wrapping the culture plate to prevent light, the bottom of the culture plate is often uneven after the culture plate is wrapped by the materials, so that the culture plate is inclined, and cell culture is affected; the used aluminum foil and tin foil can not be reused, so that waste is caused; the use is also very inconvenient and difficult to be applied practically.
At present, regarding the light-shielding culture of the cell culture plate, there are also some related patent documents, such as chinese patent CN201721429028.2 "a detachable light-shielding culture plate", which uses light-shielding material to directly make the cell culture plate, although the light-shielding purpose can be achieved, the cells can not be observed under the direct mirror, only the cell slide is taken out, the observation can be performed, and the feasibility in the actual scientific research operation is poor; also, as CN202020177582.1, "a light-resistant cell culture plate", has a complex structure, is difficult to be manufactured and produced in batches in an industrialized manner, is a disposable product, increases the cost of researchers, and has poor feasibility.
Therefore, it is necessary to design a special cassette that has a simple structure, is easy and convenient to operate, has a good light-shielding effect, and can be applied to a common cell culture plate, so as to solve the above problems.
Disclosure of Invention
The invention provides a special cassette for a cell culture plate, which is suitable in size, can be used for placing conventional cell culture plates in the market, realizes omnibearing light-proof culture and incubation, and has strong practicability; and can be used repeatedly, reduce cost.
In order to achieve the purpose, the invention adopts the following technical scheme:
the utility model provides a special magazine of cell culture board, includes base, lid, the base is equipped with the recess that is used for placing the cell culture board, is equipped with in the recess and can dismantle the water-stop sheet, and the recess top is equipped with the inboard structural slab of protruding in the base, be provided with the label district on the lid.
Preferably, one side of the base is connected with the box cover through the rotating shaft, the other side of the base is provided with a clamping groove, and the box cover is provided with a bayonet matched with the clamping groove.
Preferably, the bayonet is connected with the box cover through a bayonet rotating shaft.
Preferably, the middle positions of the front side and the rear side of the inner structure plate are provided with arc-shaped recesses for facilitating taking and placing of the cell culture plate.
Preferably, the side edge of the water-stop sheet is provided with a semicircular recess so as to facilitate the water-stop sheet to be taken out.
Preferably, the water-stop sheet is provided with a plurality of small round holes.
Preferably, the base, the box cover and the inner side structural plate are made of light-proof plastic materials.
Preferably, a gap is formed between the water-stop sheet and the bottom of the groove.
Preferably, water is filled between the water-stop sheet and the bottom of the groove.
Preferably, the top of the box cover is provided with a square protrusion, and the bottom of the base is provided with a square groove matched with the square protrusion of the box cover.
Due to the structure, the invention has the advantages that:
according to the size of a cell culture plate commonly used in the market, a square light-proof box with a proper size is specially made for placing the cell culture plate therein, so that light-proof culture and incubation are realized; the light-shielding box can be repeatedly used, so that the cost is reduced; the surface is provided with a label position, so that the light-shading plate can be conveniently marked; the bottom is provided with a groove, the plate is covered with a bulge, so that the plate is convenient to vertically stack, the stability is realized, and the space is saved.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below.
FIG. 1 is a schematic view of the overall structure of the present invention in a closed state;
FIG. 2 is a schematic view of the overall structure of the present invention in an open state;
FIG. 3 is a front view of FIG. 1;
FIG. 4 is a right side view of FIG. 1;
FIG. 5 is a rear view of FIG. 1;
FIG. 6 is a top view of FIG. 1;
FIG. 7 is a bottom view of FIG. 1;
FIG. 8 is a front view of the base of the present invention;
FIG. 9 is a top view of the base of the present invention;
FIG. 10 is a schematic view of the internal structure of the base of the present invention;
fig. 11 is a schematic structural view of a water-stop sheet according to the present invention.
Detailed Description
The technical solution of the present invention will be clearly and completely described below with reference to the accompanying drawings of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1 to 11, the present embodiment provides a cassette special for cell culture plates, which includes a base 2 and a box cover 1, wherein the base 2 is provided with a groove 11 for placing a cell culture plate, a detachable water-stop plate 9 is arranged in the groove 11, an inner side structural plate 8 protruding from the base 2 is arranged at the top of the groove 11, and the box cover 1 is provided with a label area 7.
In this embodiment, base 2 one side is connected with lid 1 through pivot 6, and the opposite side is equipped with draw-in groove 3, be equipped with on the lid 1 with draw-in groove 3 matched with bayonet socket 4, can make draw-in groove 3 just block on.
In this embodiment, the bayonet 4 is connected to the box cover 1 through a bayonet rotating shaft 5.
In this embodiment, the middle positions of the front and rear sides of the inner structural plate 8 are provided with arc-shaped recesses, so that the cell culture plate can be conveniently taken and placed.
In this embodiment, a semicircular recess 10 is formed in a side edge of the water stop sheet 9, so that the water stop sheet 9 can be conveniently taken out.
In this embodiment, the water-stop sheet 9 is provided with a plurality of small circular holes.
In this embodiment, the base 2, the box cover 1, and the inner side structural plate 8 are made of light-shielding plastic materials, and the cassette is closed to completely shield light.
In this embodiment, be equipped with the interval between water-stop sheet 9 and the recess 11 bottom, have 2cm height between water-stop sheet 9 and the recess 11 bottom, can add water between water-stop sheet 9 and recess 11 bottom like this.
In this embodiment, the top of the box cover 1 is provided with a square protrusion, and the bottom of the base 2 is provided with a square groove matched with the square protrusion of the box cover 1.
The inner side space size of the inner side structure plate 8 and the water-stop plate 9 of the embodiment is consistent with that of the conventional cell culture plate in the market, so that the cell culture plate can be stably placed conveniently and prevented from sliding in a cassette; 2cm high is arranged between the water stop sheet 9 and the groove 11 of the base 2, water can be added, and meanwhile, a small round hole is designed on the water stop sheet 9, so that the humidity in the box can be kept in the cell incubation process, and the cell climbing sheet in the culture plate can be prevented from falling into the water; a semicircular depression 10 is formed in the middle of the front part and the rear part of the water-stop sheet 9, and fingers can extend into the semicircular depression to facilitate the taking out of the water-stop sheet 9; the inner side structural plate 8 is higher, so that when the cassette is closed, light can be effectively prevented from penetrating into the cassette from a gap between the cassette cover 1 and the base 2; the front and back middle parts of the inner side structural plate 8 are provided with arc-shaped depressions, fingers can extend into the depressions, and the cell culture plate can be taken out stably; the snap-on design of the clamping groove 3 and the bayonet 4 ensures that the cassette is more stable after being closed, and the box cover 1 cannot easily fall off; the bottom design has square recess, and lid 1 top design has square protrusion, and a plurality of magazine accessible lid 1 are folded perpendicularly with the unsmooth design of base 2, lay firmly, and upper and lower magazine should not misplace or fall.
According to the size of a cell culture plate commonly used in the market, a square light-proof box with a proper size is specially made for placing the cell culture plate therein, so that light-proof culture and incubation are realized; the light-shielding box can be repeatedly used, so that the cost is reduced; the surface is provided with a label position, so that the light-shading plate can be conveniently marked; the bottom is provided with a groove, the plate is covered with a bulge, so that the plate is convenient to vertically stack, the stability is realized, and the space is saved.
The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and various modifications and changes will occur to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A special magazine of cell culture board which characterized in that: including base, lid, the base is equipped with the recess that is used for placing the cell culture board, is equipped with in the recess and dismantles the water-stop sheet, and the recess top is equipped with protruding inboard structural slab in the base, be provided with the label district on the lid.
2. The cassette of claim 1, wherein: one side of the base is connected with the box cover through a rotating shaft, the other side of the base is provided with a clamping groove, and the box cover is provided with a bayonet matched with the clamping groove.
3. The cassette of claim 2, wherein: the bayonet is connected with the box cover through a bayonet rotating shaft.
4. The cassette of claim 1, wherein: the middle positions of the front side and the rear side of the inner side structure plate are provided with arc-shaped depressions so as to be convenient for taking and placing the cell culture plate.
5. The cassette of claim 1, wherein: and the side edge of the water-stop sheet is provided with a semicircular recess so as to be convenient for taking out the water-stop sheet.
6. The cassette of claim 1, wherein: and a plurality of small round holes are formed in the water-stop sheet.
7. The cassette of claim 1, wherein: the base, the box cover and the inner side structural plate are made of light-proof plastic materials.
8. The cassette of claim 1, wherein: and a gap is arranged between the water-stop sheet and the bottom of the groove.
9. The cassette of claim 8, wherein: and water is filled between the water-stop sheet and the bottom of the groove.
10. The cassette of claim 1, wherein: the top of the box cover is provided with a square protrusion, and the bottom of the base is provided with a square groove matched with the square protrusion of the box cover.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111484701.3A CN114231412A (en) | 2021-12-07 | 2021-12-07 | Special magazine of cell culture board |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111484701.3A CN114231412A (en) | 2021-12-07 | 2021-12-07 | Special magazine of cell culture board |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114231412A true CN114231412A (en) | 2022-03-25 |
Family
ID=80753678
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111484701.3A Pending CN114231412A (en) | 2021-12-07 | 2021-12-07 | Special magazine of cell culture board |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114231412A (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015121954A1 (en) * | 2014-02-14 | 2015-08-20 | 株式会社日立製作所 | Cell culture apparatus and cell culture system |
| CN204644342U (en) * | 2015-05-14 | 2015-09-16 | 国家开发投资公司 | For cultivating the microporous culture plate of photosynthetic microorganism |
| US20160032238A1 (en) * | 2013-03-13 | 2016-02-04 | Innovative Surface Technologies, Inc. | Conical devices for three-dimensional aggregate(s) of eukaryotic cells |
| CN205687949U (en) * | 2016-06-21 | 2016-11-16 | 焦作大学 | A kind of biological cell incubator |
| WO2017221591A1 (en) * | 2016-06-24 | 2017-12-28 | 学校法人 慶應義塾 | Cell processing system and cell supply method |
| CN108367290A (en) * | 2015-10-01 | 2018-08-03 | 伯克利之光生命科技公司 | Wellhole plate incubator |
| CN212693792U (en) * | 2020-08-17 | 2021-03-12 | 重庆医科大学附属儿童医院 | Cell slide immunofluorescence antibody incubation box |
| CN214167993U (en) * | 2020-11-19 | 2021-09-10 | 华中科技大学同济医学院附属协和医院 | Lock catch type cell culture plate |
| CN214952525U (en) * | 2021-05-26 | 2021-11-30 | 宁夏医科大学总医院 | Cell climbing film light-resistant dyeing incubation box |
-
2021
- 2021-12-07 CN CN202111484701.3A patent/CN114231412A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20160032238A1 (en) * | 2013-03-13 | 2016-02-04 | Innovative Surface Technologies, Inc. | Conical devices for three-dimensional aggregate(s) of eukaryotic cells |
| WO2015121954A1 (en) * | 2014-02-14 | 2015-08-20 | 株式会社日立製作所 | Cell culture apparatus and cell culture system |
| CN204644342U (en) * | 2015-05-14 | 2015-09-16 | 国家开发投资公司 | For cultivating the microporous culture plate of photosynthetic microorganism |
| CN108367290A (en) * | 2015-10-01 | 2018-08-03 | 伯克利之光生命科技公司 | Wellhole plate incubator |
| CN205687949U (en) * | 2016-06-21 | 2016-11-16 | 焦作大学 | A kind of biological cell incubator |
| WO2017221591A1 (en) * | 2016-06-24 | 2017-12-28 | 学校法人 慶應義塾 | Cell processing system and cell supply method |
| CN212693792U (en) * | 2020-08-17 | 2021-03-12 | 重庆医科大学附属儿童医院 | Cell slide immunofluorescence antibody incubation box |
| CN214167993U (en) * | 2020-11-19 | 2021-09-10 | 华中科技大学同济医学院附属协和医院 | Lock catch type cell culture plate |
| CN214952525U (en) * | 2021-05-26 | 2021-11-30 | 宁夏医科大学总医院 | Cell climbing film light-resistant dyeing incubation box |
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