CN212693792U - Cell slide immunofluorescence antibody incubation box - Google Patents
Cell slide immunofluorescence antibody incubation box Download PDFInfo
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- CN212693792U CN212693792U CN202021705690.8U CN202021705690U CN212693792U CN 212693792 U CN212693792 U CN 212693792U CN 202021705690 U CN202021705690 U CN 202021705690U CN 212693792 U CN212693792 U CN 212693792U
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Abstract
The utility model provides a cell climbing sheet immunofluorescence antibody incubation box, which comprises an opaque box body and a box cover, namely, the box body adopts a hidden box body, the box cover adopts a hidden box cover, an internal part and a cylindrical part are arranged in the box body, the cylindrical part is vertically inserted on the internal part, the top of the cylindrical part is lower than the top wall of the box cover, and the outer diameter of the cylindrical part is smaller than the outer diameter of the cell climbing sheet and is more than one half of the outer diameter of the cell climbing sheet; a space for containing water is also arranged in the box body, and the liquid level of the contained water is lower than the top end of the cylindrical part. Adopt the utility model discloses when hatching anti, with the help of the gravity of cell climbing piece dead weight and antibody, can make cell climbing piece middle part slightly recessed, this not only can ensure that the antibody stays on the cell climbing piece, helps the cell climbing piece to arrange the cylindricality portion top in steadily in addition, the retaining effect of the water of splendid attire especially sponge in the box body then provides moist environment, can guarantee that the antibody effectively combines and prevent that the climbing piece from becoming dry, can prevent to get the in-process wounded hand of putting the cylindricality portion simultaneously.
Description
Technical Field
The utility model relates to an incubate the box, especially relate to a box is hatched to cell slide immunofluorescence antibody.
Background
In the immunofluorescence experiment using the cell slide, the existing method is to place the cell slide in a cell plate to incubate a primary antibody, and this needs to use more antibodies, which is a waste. In addition, document CN2009200807195 provides a cell and cell slide integrated preparation box, which comprises a culture box, a cell slide, and a slide bracket used for being placed in the culture box: the bottom of the square frame-shaped support base is provided with support legs, a plurality of climbing film fixing strips are fixed on the support base at equal intervals in parallel, the narrowing parts of the I-shaped cross sections of the fixing strips form climbing film placing grooves, and the climbing film placing grooves on the two adjacent fixing strips are used for placing cell climbing films. Document CN201420294711 provides a dedicated immunochemistry color developing box for cell slide, which comprises a box cover and a box body, wherein the box cover is a light-shading box cover, the side surface and the bottom surface of the box body are light-shading and transparent, a water storage tank and a slide groove are arranged at the inner bottom of the box body, and a light-shading bottom plate which can open the bottom of the box body for light transmission and can close the bottom of the box body for light shading is arranged at the outer bottom of the box body. However, the antibody incubation cassette provided in the above document has the problem of inconvenient operation, and the manufacturing cost of the cassette is relatively high.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide an antibody quantity is few, and convenient operation and cost of manufacture are low cell slide immunofluorescence antibody incubate box.
In order to achieve the above purpose, the present invention adopts the following technical solution.
The utility model provides a box is hatched to cell slide immunofluorescence antibody, includes lightproof box body and lid, and the box body adopts dark box body promptly, and the lid adopts dark lid, its characterized in that: the cell climbing sheet box is characterized in that an internal part and a cylindrical part are arranged in the box body, the cylindrical part is vertically inserted into the internal part, the top of the cylindrical part is lower than the top wall of the box cover, and the outer diameter of the cylindrical part is smaller than the outer diameter of the cell climbing sheet and is larger than one half of the outer diameter of the cell climbing sheet; a space for containing water is also arranged in the box body, and the liquid level of the contained water is lower than the top end of the cylindrical part.
As the preferred scheme, the cylindricality portion adopts the hollow formula barrel, and the bottom of cylindricality portion is closed structure, and the cavity intracavity of cylindricality portion is equipped with water.
Preferably, the cylindrical part is a hollow cylinder with two open ends, and the bottom end of the cylindrical part is directly submerged below the liquid level so that water can enter the hollow cavity of the cylindrical part.
Preferably, the column part is of a solid column structure, the top of the column part is provided with a concave part, and the bottom end of the column part is submerged below the liquid level.
Preferably, the built-in part is made of sponge with holes, and the cylindrical part is inserted in the holes.
As the preferred scheme, the built-in part clamps at the box body lateral wall, and the built-in part bottom is unsettled, and cylindricality portion bottom is less than the built-in part bottom, and cylindricality portion bottom submerges below the liquid level.
Adopt the utility model provides a scheme, when hatching one anti, only need earlier with cylindricality portion cartridge on built-in to add suitable water in the box body, place the cell climbing piece on cylindricality portion top again, then drip the antibody at the cell climbing piece, cover at last after the lid put into after cultivate the environment can, after hatching, only need open the lid, directly take off the cell climbing piece, whole operation process is very simple and convenient, and required antibody is very few, a cell climbing piece only need use one or two to drip. Because the cylindricality portion is mesoporium structure or cylindricality portion top has the concave part down, after putting the cell climbing piece and dripping the antibody, with the help of the gravity of cell climbing piece dead weight and antibody, can make cell climbing piece middle part slightly recessed, this not only can ensure that the antibody is stayed on the cell climbing piece, and help the cell climbing piece to place the cylindricality portion top in steadily, the water storage effect of the water of splendid attire especially sponge in the box body then provides moist environment, can guarantee that the antibody effectively combines and prevent the climbing piece from drying, can prevent to get the in-process wounded hand of putting cylindricality portion simultaneously, the space that leaves between box body top and the lid then allows the antibody fully to combine on the cell climbing piece. The utility model provides a box is hatched to cell slide immunofluorescence antibody mainly includes cylindricality portion, sponge and magazine, its simple structure, and the cost of manufacture is low.
Drawings
FIG. 1 is a schematic diagram of a cell slide immunofluorescent antibody incubation cassette in one embodiment;
FIG. 2 is a schematic diagram of a cell slide immunofluorescent antibody incubation cassette II in the example;
FIG. 3 is a third schematic diagram of the immunofluorescent antibody incubation cassette of the cell slide in the example;
FIG. 4 is a fourth schematic diagram of the immunofluorescent antibody incubation cassette of the cell slide in the example;
FIG. 5 is a top schematic view of the cylindrical portion of FIG. 4;
FIG. 6 is a schematic diagram of the cell slide immunofluorescent antibody incubation cassette in the example shown in the top view (without the cassette cover).
Detailed Description
The present invention will be further described with reference to the accompanying drawings and specific embodiments, but the following description of the embodiments is only for the purpose of helping understanding the principle and the core idea of the present invention, and is not intended to limit the scope of the present invention. It should be noted that modifications to the present invention may occur to those skilled in the art without departing from the principles of the present invention and are intended to fall within the scope of the appended claims.
Examples
Referring to fig. 1 to 6, a cell slide immunofluorescence antibody incubation box, includes lightproof box body 1 and lid 4, and box body 1 is connected with lid 4 lock, and box body 1 and lid 4 are preferred to be wrapped with the tinfoil, can keep out of the sun after covering, avoids being decomposed by the illumination and weakens fluorescence effect, influences the experimental result. The cell climbing sheet box is characterized in that an inner part 6 and a cylindrical part 5 are arranged in the box body 1, the cylindrical part 5 is vertically inserted into the inner part 6, the top of the cylindrical part 5 is lower than the top wall of the box cover 4, and the outer diameter of the cylindrical part 5 is smaller than the outer diameter of the cell climbing sheet 3 and is larger than one half of the outer diameter of the cell climbing sheet 3; a space for containing water is also arranged in the box body 1, and the liquid level 7 of the contained water is lower than the top end of the cylindrical part 5.
In a specific embodiment, as shown in fig. 1 and 2, the cylindrical part 5 is a hollow cylinder, the bottom end of the cylindrical part 5 is a closed structure, and water is contained in the hollow cavity of the cylindrical part 5. In fig. 1, the cylindrical portion 5 extends into the lower middle portion of the insert 6; in fig. 2, the cylindrical portion 5 extends into the bottom of the insert 6 and is supported by the bottom of the cartridge 1.
In another embodiment, as shown in fig. 3, the column part 5 is a hollow cylinder with two open ends, and the bottom end of the column part 5 is submerged directly below the liquid level 7 so that water can enter the hollow cavity of the column part 5. In fig. 3, the internal member 6 is clamped on the side wall of the box body 1, the bottom of the internal member 6 is suspended, the bottom end of the column part 5 is lower than the bottom end of the internal member 6, and the bottom end of the column part 5 is submerged below the liquid level 7.
In another embodiment, as shown in fig. 4 and 5, the cylindrical part 5 is a solid cylinder structure, the top of the cylindrical part 5 is provided with a lower concave part 8, and the bottom end of the cylindrical part 5 is submerged directly below the liquid level 7.
In the various solutions applied in the present embodiment, the insert 6 is a sponge with a hole, and the cylindrical portion 5 is inserted in the hole.
When hatching an anti, only need with 5 cartridge of cylindricality portion on built-in 6, and add the right amount of water in the box body 1, place cell climbing piece 3 on 5 tops of cylindricality portion again, then drop antibody 2 at cell climbing piece 3, put into after covering lid 4 at last and cultivate the environment can, after hatching, only need open lid 4, directly take off cell climbing piece 3, whole operation process is very simple and convenient, and required antibody 2 is very few, a cell climbing piece 3 only need use one or two drops of antibodies usually. Because the column part 5 is the mesohole structure or the top of the column part 5 is provided with the concave part 8, after the cell climbing sheet 3 is placed and the antibody 2 is dripped, the middle part of the cell climbing sheet 3 is slightly concave by means of the dead weight of the cell climbing sheet 3 and the gravity of the antibody 2, the middle part of the cell climbing sheet 3 can be enabled to be slightly concave, the antibody 2 can be ensured to be left on the cell climbing sheet 3, and the cell climbing sheet 3 is helped to be stably placed on the top of the column part 5, the water storage effect of the water contained in the box body 1, particularly the sponge, provides a wetting environment, the effective combination of the antibody 2 and the drying of the climbing sheet can be ensured, meanwhile, the hands can be prevented from being injured in the process of taking and placing the column part 5, and the space left between the. The utility model provides a box is hatched to 3 immunofluorescence antibodies 2 of cell slide mainly includes cylindricality portion 5, sponge and magazine, its simple structure, and the cost of manufacture is low.
Finally, it should be noted that the column parts 5 on the insert 6 are set by those skilled in the art according to actual conditions, and 6 column parts 5, 12 column parts 5, 24 column parts 5, and 48 column parts 5 may be provided on the insert 6. The dropping antibody is determined according to the size of the cell slide 3, about two drops of the antibody are dropped when the cell slide with 24 holes is used (24 column parts 5), the cell slides with other specifications can be flexibly adjusted according to the actual situation, but the using amount of the antibody is less than that of the antibody which is placed in a cell plate for incubation on the whole.
Claims (6)
1. The utility model provides a box is hatched to cell slide immunofluorescence antibody, includes opaque box body (1) and lid (4), its characterized in that: the cell climbing sheet is characterized in that an internal part (6) and a cylindrical part (5) are arranged in the box body (1), the cylindrical part (5) is vertically inserted into the internal part (6), the top of the cylindrical part (5) is lower than the top wall of the box cover (4), and the outer diameter of the cylindrical part (5) is smaller than the outer diameter of the cell climbing sheet (3) and is larger than one half of the outer diameter of the cell climbing sheet (3); a space for containing water is also arranged in the box body (1), and the liquid level (7) of the contained water is lower than the top end of the cylindrical part (5).
2. The cell-slide immunofluorescent antibody incubation cartridge according to claim 1, characterized in that: the cylindrical part (5) adopts a hollow cylinder, the bottom end of the cylindrical part (5) is of a closed structure, and water is contained in the hollow cavity of the cylindrical part (5).
3. The cell-slide immunofluorescent antibody incubation cartridge according to claim 1, characterized in that: the column part (5) adopts a hollow cylinder with two open ends, and the bottom end of the column part (5) is directly submerged below the liquid level (7) so that water can enter the hollow cavity of the column part (5).
4. The cell-slide immunofluorescent antibody incubation cartridge according to claim 1, characterized in that: the column part (5) adopts a solid column structure, the top of the column part (5) is provided with a lower concave part (8), and the bottom end of the column part (5) is directly submerged below the liquid level (7).
5. The cell-slide immunofluorescent antibody incubation cartridge according to any one of claims 1-4, wherein: the built-in part (6) adopts sponge with holes, and the cylindrical part (5) is inserted in the holes.
6. The cell-slide immunofluorescent antibody incubation cartridge according to claim 5, characterized in that: the built-in part (6) is clamped on the side wall of the box body (1), the bottom of the built-in part (6) is suspended, the bottom end of the column part (5) is lower than the bottom end of the built-in part (6), and the bottom end of the column part (5) is submerged below the liquid level (7).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021705690.8U CN212693792U (en) | 2020-08-17 | 2020-08-17 | Cell slide immunofluorescence antibody incubation box |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021705690.8U CN212693792U (en) | 2020-08-17 | 2020-08-17 | Cell slide immunofluorescence antibody incubation box |
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| Publication Number | Publication Date |
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| CN212693792U true CN212693792U (en) | 2021-03-12 |
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| CN202021705690.8U Active CN212693792U (en) | 2020-08-17 | 2020-08-17 | Cell slide immunofluorescence antibody incubation box |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114231412A (en) * | 2021-12-07 | 2022-03-25 | 华中科技大学同济医学院附属协和医院 | Special magazine of cell culture board |
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2020
- 2020-08-17 CN CN202021705690.8U patent/CN212693792U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114231412A (en) * | 2021-12-07 | 2022-03-25 | 华中科技大学同济医学院附属协和医院 | Special magazine of cell culture board |
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