CN215593069U - Automatic liquid device is traded to cell - Google Patents
Automatic liquid device is traded to cell Download PDFInfo
- Publication number
- CN215593069U CN215593069U CN202121231841.5U CN202121231841U CN215593069U CN 215593069 U CN215593069 U CN 215593069U CN 202121231841 U CN202121231841 U CN 202121231841U CN 215593069 U CN215593069 U CN 215593069U
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- liquid
- box
- pump
- cell
- driving rod
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Abstract
An automatic cell liquid changing device comprises an installation bottom plate, wherein a culture medium solution box and a waste liquid collecting box are installed on the installation bottom plate, glass cover plates are arranged at the tops of the culture medium solution box and the waste liquid collecting box, and a liquid adding pump is installed on the side wall of the culture medium solution box; in conclusion, the driving rod is controlled to advance, sample adding and liquid sucking through the switch respectively, repeated labor in single sample adding liquid is avoided, efficiency is improved, repeated sample adding can be carried out on the 96-hole plate, pollution caused by manual sample adding is avoided through the new technology, sample adding amount of different culture dishes is more accurate and constant, and the use is convenient.
Description
Technical Field
The utility model belongs to the technical field of cell biology, and particularly relates to an automatic cell liquid changing device.
Background
Cell change liquid belongs to common operations of cell biology, but a culture medium needs to be frequently sucked when the cell change liquid is used, and if the cell change liquid is improperly sucked, pollution is easily caused to influence the progress of a subsequent experiment, so that a lot of inconvenience exists; therefore, it is highly desirable to design a device capable of automatically changing the liquid so that the cell liquid can be changed more easily, cleanly and accurately.
SUMMERY OF THE UTILITY MODEL
The utility model aims to overcome the defects in the prior art and provides the automatic cell liquid changing device, which is convenient for experimenters to repeatedly change liquid, saves labor cost, reduces pollution and is more convenient and accurate in liquid changing.
In order to solve the technical problems, the utility model adopts the following technical scheme: an automatic cell liquid changing device comprises an installation bottom plate, wherein a culture medium solution box and a waste liquid collecting box are installed on the installation bottom plate, glass cover plates are arranged at the tops of the culture medium solution box and the waste liquid collecting box respectively, a liquid adding pump is installed on the side wall of the culture medium solution box, a liquid inlet of the liquid adding pump is connected with the culture medium solution box through a pipeline, a liquid pumping pump is installed on the side wall of the waste liquid collecting box, and a liquid outlet of the liquid pumping pump is connected with the waste liquid collecting box through a pipeline;
the mounting bottom plate is also provided with a plurality of cell culture dish clamping grooves, all the cell culture dish clamping grooves are arranged in a rectangular array, and cell culture dishes are placed in the cell culture dish clamping grooves; the cell liquid changing integrated mechanism is connected with the liquid adding pump and the liquid drawing pump respectively, and is used for adding liquid and drawing liquid into the cell culture dish.
The cell liquid changing integrated mechanism comprises a driving rod and a mounting box, through holes are correspondingly formed in two opposite side box walls of the mounting box, the driving rod penetrates through the two through holes, the upper width and the lower width of each through hole are larger than the upper width and the lower width of each driving rod, a transverse pipe is fixed at the end part of each driving rod, a plurality of groups of liquid changing heads are installed at the bottom of each transverse pipe, the number of the liquid changing heads is equal to the number of one row of cell culture dish clamping grooves, the liquid changing heads are in one-to-one correspondence with the row of cell culture dish clamping grooves up and down, each group of liquid changing heads comprises a liquid suction needle tube and a sample adding needle tube, the liquid suction needle tube and the sample adding needle tube are fixedly adhered side by side, the lower end of liquid suction is lower than the lower end of a needle tube, the upper end of the liquid suction needle tube is connected with a liquid inlet of a liquid pump through a sample adding hose, and the sample adding hose are arranged in the transverse pipes;
the installation box is internally provided with a moving assembly, the moving assembly comprises a forward and reverse rotating motor and two rotating shafts, the two rotating shafts are rotatably installed in the installation box, each rotating shaft is provided with a belt wheel and a cam, the belt wheels on the two rotating shafts are in transmission connection through a belt, the forward and reverse rotating motor is in transmission connection with one of the rotating shafts through a coupler, the outer circle surface of each cam consists of an arc surface and a straight plane, a rubber layer is arranged on the arc surface, the straight plane is a smooth surface, and the outer circle surface of each cam is in contact with the bottom surface of the driving rod; the installation bottom plate is provided with a forward and reverse rotation motor control switch, a liquid feeding switch and a liquid pumping switch, the forward and reverse rotation motor control switch is used for controlling forward rotation and reverse rotation of a forward and reverse rotation motor, the liquid feeding switch is used for controlling the working of a liquid feeding pump, and the liquid pumping switch is used for controlling the working of a liquid pumping pump.
An elastic pad is arranged in each perforation and provided with a smooth inner ring, and the driving rod penetrates through the smooth inner ring of the elastic pad.
By adopting the technical scheme, the utility model has the following beneficial effects: the utility model respectively controls the driving rod to advance, sample adding and liquid absorbing through the switch, avoids repeated labor in single sample adding liquid, improves the efficiency, can repeatedly add samples to the 96-hole plate, avoids pollution increased in manual sample adding, and has more accurate and constant sample adding amount of different culture dishes and convenient use.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is an enlarged view at A in FIG. 1;
fig. 3 is a schematic view of the installation of the moving assembly.
Detailed Description
As shown in fig. 1-3, the automatic cell liquid changing device of the present invention comprises an installation base plate 1, a culture medium solution box 2 and a waste liquid collecting box 3 are installed on the installation base plate 1, glass cover plates 4 are respectively installed on the tops of the culture medium solution box 2 and the waste liquid collecting box 3, a liquid adding pump 5 is installed on the side wall of the culture medium solution box 2, a liquid inlet of the liquid adding pump 5 is connected with the culture medium solution box 2 through a pipeline, a liquid pumping pump 6 is installed on the side wall of the waste liquid collecting box 3, and a liquid outlet of the liquid pumping pump 6 is connected with the waste liquid collecting box 3 through a pipeline;
the mounting bottom plate 1 is also provided with a plurality of cell culture dish clamping grooves 7, all the cell culture dish clamping grooves 7 are arranged in a rectangular array, and cell culture dishes 8 are placed in the cell culture dish clamping grooves 7; the mounting base plate 1 is also provided with a cell liquid-changing integrated mechanism which is respectively connected with a liquid adding pump 5 and a liquid drawing pump 6, and the cell liquid-changing integrated mechanism is used for adding liquid and drawing liquid into a cell culture dish 8.
The cell liquid changing integrated mechanism comprises a driving rod 9 and a mounting box 10, through holes 11 are correspondingly formed in two opposite side box walls of the mounting box 10, the driving rod 9 is arranged in the two through holes 11 in a penetrating mode, the width of the upper portion and the lower portion of each through hole 11 is larger than the width of the upper portion and the lower portion of each driving rod 9, a transverse pipe 12 is fixed to the end portion of each driving rod 9, a plurality of groups of liquid changing heads 13 are mounted at the bottom of each transverse pipe 12, the number of the liquid changing heads 13 is equal to the number of a row of clamping grooves 7 of a cell culture dish 8, the liquid changing heads 13 correspond to the row of clamping grooves 7 of the cell culture dish 8 one by one, each group of the liquid changing heads 13 comprises a liquid suction needle tube 14 and a sample adding needle tube 15, the liquid suction needle tube 14 and the sample adding needle tube 15 are fixedly adhered side by side, the lower end of the liquid suction needle tube 14 is lower than the lower end of the sample adding needle tube 15, the upper end of the liquid suction needle tube 14 is connected with a liquid inlet of the liquid pump 6 through a liquid adding hose, the upper end of the sample adding needle tube 15 is connected with a liquid outlet of the liquid adding pump 5 through the sample adding hose, the sample adding hose and the liquid suction hose are arranged in the transverse pipe 12;
a moving assembly is arranged in the mounting box 10 and comprises a forward and reverse rotating motor and two rotating shafts 16, the two rotating shafts 16 are rotatably arranged in the mounting box 10, each rotating shaft 16 is provided with a belt wheel 17 and a cam 18, the belt wheels 17 on the two rotating shafts 16 are in transmission connection through a belt, the forward and reverse rotating motor is in transmission connection with one of the rotating shafts 16 through a coupling, the outer circle surface of each cam 18 consists of an arc surface 19 and a straight plane 20, a rubber layer is arranged on the arc surface 19, the straight plane 20 is a smooth surface, and the outer circle surface of each cam 18 is in contact with the bottom surface of the driving rod 9; the installation bottom plate 1 is provided with a forward and reverse rotation motor control switch, a liquid feeding switch and a liquid pumping switch, the forward and reverse rotation motor control switch is used for controlling forward rotation and reverse rotation of a forward and reverse rotation motor, the liquid feeding switch is used for controlling the working of the liquid feeding pump 5, and the liquid pumping switch is used for controlling the working of the liquid pumping pump 6.
An elastic pad 21 is arranged in each through hole 11, the elastic pad 21 is provided with a smooth inner ring, and the driving rod 9 passes through the smooth inner ring of the elastic pad 21.
The culture medium solution box 2 is used for containing cell culture solution, the cell culture solution can be pumped into the sample adding needle tube 15 through the liquid adding pump 5, the waste liquid collecting box 3 is used for containing waste liquid, and the waste liquid in the cell culture dish 8 can be pumped into the waste liquid collecting box 3 through the liquid pumping pump 6;
for example, when the sample adding operation of the cell culture dish 8 is needed, the forward and reverse rotating motor is started to drive the two groups of belt wheels 17 to rotate, so that the two cams 18 rotate in the same direction, the cams 18 rotate to push the driving rod 9 forward by utilizing the friction force between the arc surface 19 and the driving rod 9 until the liquid changing head 13 reaches the slot 7 of one row of cell culture dishes 8, the forward and reverse rotating motor is stopped, the liquid adding switch is pressed, and the liquid adding pump 5 can inject the cell culture liquid into the cell culture dish 8 through the sample adding needle tube 15; when the cell liquid in the cell culture dish 8 reaches a certain amount, stopping adding the liquid, starting the forward and reverse rotating motor again, driving the driving rod 9 to move forward for a certain distance by utilizing the cam 18, and adding the liquid to the next row of cell culture dishes 8; in addition, when the cam 18 drives the driving rod 9 to move forward, due to the special structure of the cam 18, the driving rod 9 will rise upward by a certain height, in the process, the liquid changing head 13 is lifted upward from the cell culture dish 8, then the cam 18 rotates to move the driving rod 9 forward until the next row of cell culture dishes 8, the straight plane 20 of the cam 18 contacts with the driving rod 9, the driving rod 9 falls by a certain height, and the liquid changing head 13 falls into the cell culture dishes 8; in the whole process, the driving rod 9 can vibrate due to the special structure of the cam 18, so that the elastic pad 21 is arranged at the through hole 11, the vibration of the driving rod 9 can be reduced to a great extent, and the phenomenon that liquid in the liquid changing head 13 falls on the mounting bottom plate 1 to cause pollution due to the vibration of the driving rod 9 is avoided.
Imbibition process is similar with above-mentioned application of sample process, no longer gives unnecessary details, and the lower extreme of imbibition needle tubing 14 is less than the lower extreme of application of sample needle tubing 15 in addition, and the aim at that sets up like this, imbibition needle tubing 14 are when the suction waste liquid, and the waste liquid can not contact application of sample needle tubing 15, avoids polluting application of sample needle tubing 15.
The present embodiment is not intended to limit the shape, material, structure, etc. of the present invention in any way, and any simple modification, equivalent change and modification made to the above embodiments according to the technical spirit of the present invention are within the scope of the technical solution of the present invention.
Claims (3)
1. An automatic cell liquid changing device is characterized in that: the culture medium liquid feeding device comprises an installation bottom plate, wherein a culture medium solution box and a waste liquid collecting box are installed on the installation bottom plate, glass cover plates are arranged at the tops of the culture medium solution box and the waste liquid collecting box respectively, a liquid feeding pump is installed on the side wall of the culture medium solution box, a liquid inlet of the liquid feeding pump is connected with the culture medium solution box through a pipeline, a liquid suction pump is installed on the side wall of the waste liquid collecting box, and a liquid outlet of the liquid suction pump is connected with the waste liquid collecting box through a pipeline;
the mounting bottom plate is also provided with a plurality of cell culture dish clamping grooves, all the cell culture dish clamping grooves are arranged in a rectangular array, and cell culture dishes are placed in the cell culture dish clamping grooves; the cell liquid changing integrated mechanism is connected with the liquid adding pump and the liquid drawing pump respectively, and is used for adding liquid and drawing liquid into the cell culture dish.
2. An automatic cell exchange fluid device according to claim 1, wherein: the cell liquid changing integrated mechanism comprises a driving rod and a mounting box, through holes are correspondingly formed in two opposite side box walls of the mounting box, the driving rod penetrates through the two through holes, the upper width and the lower width of each through hole are larger than the upper width and the lower width of each driving rod, a transverse pipe is fixed at the end part of each driving rod, a plurality of groups of liquid changing heads are installed at the bottom of each transverse pipe, the number of the liquid changing heads is equal to the number of one row of cell culture dish clamping grooves, the liquid changing heads are in one-to-one correspondence with the row of cell culture dish clamping grooves up and down, each group of liquid changing heads comprises a liquid suction needle tube and a sample adding needle tube, the liquid suction needle tube and the sample adding needle tube are fixedly adhered side by side, the lower end of liquid suction is lower than the lower end of a needle tube, the upper end of the liquid suction needle tube is connected with a liquid inlet of a liquid pump through a sample adding hose, and the sample adding hose are arranged in the transverse pipes;
the installation box is internally provided with a moving assembly, the moving assembly comprises a forward and reverse rotating motor and two rotating shafts, the two rotating shafts are rotatably installed in the installation box, each rotating shaft is provided with a belt wheel and a cam, the belt wheels on the two rotating shafts are in transmission connection through a belt, the forward and reverse rotating motor is in transmission connection with one of the rotating shafts through a coupler, the outer circle surface of each cam consists of an arc surface and a straight plane, a rubber layer is arranged on the arc surface, the straight plane is a smooth surface, and the outer circle surface of each cam is in contact with the bottom surface of the driving rod; the installation bottom plate is provided with a forward and reverse rotation motor control switch, a liquid feeding switch and a liquid pumping switch, the forward and reverse rotation motor control switch is used for controlling forward rotation and reverse rotation of a forward and reverse rotation motor, the liquid feeding switch is used for controlling the working of a liquid feeding pump, and the liquid pumping switch is used for controlling the working of a liquid pumping pump.
3. An automatic cell exchange fluid device according to claim 2, wherein: an elastic pad is arranged in each perforation and provided with a smooth inner ring, and the driving rod penetrates through the smooth inner ring of the elastic pad.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202121231841.5U CN215593069U (en) | 2021-06-03 | 2021-06-03 | Automatic liquid device is traded to cell |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202121231841.5U CN215593069U (en) | 2021-06-03 | 2021-06-03 | Automatic liquid device is traded to cell |
Publications (1)
Publication Number | Publication Date |
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CN215593069U true CN215593069U (en) | 2022-01-21 |
Family
ID=79874333
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN202121231841.5U Expired - Fee Related CN215593069U (en) | 2021-06-03 | 2021-06-03 | Automatic liquid device is traded to cell |
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CN (1) | CN215593069U (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115369034A (en) * | 2022-08-18 | 2022-11-22 | 潍坊医学院 | Liquid-changing type multi-cell co-culture simulated weightlessness experimental device |
-
2021
- 2021-06-03 CN CN202121231841.5U patent/CN215593069U/en not_active Expired - Fee Related
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115369034A (en) * | 2022-08-18 | 2022-11-22 | 潍坊医学院 | Liquid-changing type multi-cell co-culture simulated weightlessness experimental device |
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CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20220121 |