CN215586802U - Cell separation device for flow cytometry analysis - Google Patents

Cell separation device for flow cytometry analysis Download PDF

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Publication number
CN215586802U
CN215586802U CN202122194239.5U CN202122194239U CN215586802U CN 215586802 U CN215586802 U CN 215586802U CN 202122194239 U CN202122194239 U CN 202122194239U CN 215586802 U CN215586802 U CN 215586802U
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China
Prior art keywords
test tube
fixedly connected
flow cytometry
cell separation
rubber
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CN202122194239.5U
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Chinese (zh)
Inventor
杨晓丽
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Shandong Amber Biotechnology Co ltd
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Shandong Amber Biotechnology Co ltd
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Priority to CN202122194239.5U priority Critical patent/CN215586802U/en
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Abstract

The utility model discloses a cell separation device for flow cytometry analysis, and relates to the field of cell separation devices. This a cell separator for flow cytometry uses includes rotation chassis and chucking fixed establishment, and rotation chassis's top fixedly connected with multiunit test tube base, the inside activity joint of test tube base have the rubber chucking cover, and the activity joint has the test tube in the rubber chucking cover. This a cell separator for flow cytometry usefulness is when using, the convenience is fixed the test tube, and utilize chucking fixed establishment to press from both sides tight fixedly to the test tube, make the test tube stronger with the wholeness of test tube base and cutting ferrule, when rotatory chassis is high-speed rotatory, it is rotatory to drive test tube base and test tube, carry out centrifugal treatment to the cell suspension in the test tube, thereby be convenient for carry out the cell separation, in the processing procedure, not hard up phenomenon can not appear in the test tube, guarantee whole cell separator's stability.

Description

Cell separation device for flow cytometry analysis
Technical Field
The utility model relates to the technical field of cell separation devices, in particular to a cell separation device for flow cytometry analysis.
Background
Flow cytometry is a technology for rapidly and quantitatively analyzing and sorting single cells, wherein in the analyzing or sorting process, cells wrapped in sheath fluid form liquid drops containing the single cells through a nozzle controlled by high-frequency oscillation, and the cells emit scattered light and fluorescence under the irradiation of laser beams, are detected by a detector, are converted into electric signals, are sent into a computer for processing, and output statistical results.
In the process of carrying out flow cytometry, cells generally need to be separated, a cell separation device is mostly used, a cell centrifuge is mostly used for operation in the cell separation device, when the existing cell separation device is used, a test tube generally needs to be placed in a test tube sleeve of the cell centrifuge, then centrifugal operation is realized under high-speed rotation of a rotating chassis, and finally cell separation is realized.
SUMMERY OF THE UTILITY MODEL
Technical problem to be solved
In view of the shortcomings of the prior art, the present invention discloses a cell separation device for flow cytometry analysis, which solves the problems mentioned in the background art.
(II) technical scheme
In order to achieve the purpose, the utility model is realized by the following technical scheme: a cell separation device for flow cytometry analysis, comprising:
the test tube test device comprises a rotating chassis, wherein the top of the rotating chassis is fixedly connected with a plurality of groups of test tube bases, rubber clamping sleeves are movably clamped inside the test tube bases, and test tubes are movably clamped in the rubber clamping sleeves;
the clamping and fixing mechanism comprises a fixing column, the fixing column is fixedly connected to the side wall of the test tube base, the end portion of the fixing column is fixedly connected with a plane plate, the center of the plane plate is fixedly connected with a nut sleeve, the inner thread of the nut sleeve is connected with a lead screw, the end portion of the lead screw is rotatably connected with a sliding block through a bearing, and the side wall of the sliding block is fixedly connected with a rubber clamping block.
Preferably, the lateral wall of test tube base is seted up the first notch that corresponds with rubber clamp block, the lateral wall of rubber clamping sleeve is seted up the second notch that corresponds with rubber clamp block.
Preferably, the lateral wall fixedly connected with cutting ferrule of test tube base, the lateral wall fixedly connected with card strip of rubber clamping sleeve, card strip activity joint is in the cutting ferrule.
Preferably, a central column is fixedly connected to the center of the top of the rotating chassis, a reinforcing column is fixedly connected to the side wall of the central column, and the reinforcing column is fixedly connected to the test tube base.
Preferably, a round hole is formed in the sliding block, and the sliding block is movably sleeved on the fixed column through the round hole.
Preferably, the side wall of the rubber clamping block is fixedly connected with a plurality of groups of anti-slip strips, and one end of the screw rod, far away from the sliding block, is fixedly connected with a rotating handle.
The utility model discloses a cell separation device for flow cytometry, which has the following beneficial effects:
1. this a cell separator for flow cytometry is used, when using, the convenience is fixed the test tube, and utilize chucking fixed establishment to press from both sides tight fixedly to the test tube, make the test tube stronger with the wholeness of test tube base and cutting ferrule, when rotatory chassis is high-speed rotatory, it is rotatory to drive test tube base and test tube, carry out centrifugal treatment to the cell suspension in the test tube, thereby be convenient for carry out the cell separation, in the processing procedure, not hard up phenomenon can not appear in the test tube, guarantee whole cell separator's stability.
2. This a cell separator for flow cytometry usefulness, the cutting ferrule of setting and the cooperation of card strip can have avoided the tight cover of rubber card to take place spin or not hard up phenomenon in the inner chamber of test tube base, guarantees to carry out stable support to the test tube, through the center post and the enhancement post that set up, can make to consolidate whole test tube base, has guaranteed to carry out centrifugal operation at test tube base high-speed rotation and to the test tube when, test tube base's stability obtains guaranteeing.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a top view of the present invention;
FIG. 3 is an exploded view of a portion of the structure of the present invention;
FIG. 4 is a block diagram of the rubber clamping block of the present invention.
In the figure: 1. rotating the chassis; 2. a central column; 3. a test tube base; 301. a card sleeve; 302. a first notch; 4. a reinforcement column; 5. a rubber clamping sleeve; 501. clamping the strip; 502. a second notch; 6. fixing a column; 7. a flat plate; 8. a nut sleeve; 9. a screw rod; 10. a slider; 11. a bearing; 12. a rubber clamping block; 13. and (4) an anti-slip strip.
Detailed Description
The embodiment of the utility model discloses a cell separation device for flow cytometry, which comprises the following components as shown in figures 1-4:
the test tube test device comprises a rotary chassis 1, wherein the top of the rotary chassis 1 is fixedly connected with a plurality of groups of test tube bases 3, rubber clamping sleeves 5 are movably clamped in the test tube bases 3, and test tubes are movably clamped in the rubber clamping sleeves 5;
chucking fixed establishment, chucking fixed establishment include fixed column 6, and fixed column 6 fixed connection is on test tube base 3's lateral wall, and fixed column 6's tip fixedly connected with surface plate 7, the center department fixedly connected with nut cover 8 of surface plate 7, and the internal thread of nut cover 8 is connected with lead screw 9, and the tip of lead screw 9 rotates through bearing 11 and is connected with slider 10, slider 10's lateral wall fixedly connected with rubber clamp tight piece 12.
As shown in fig. 1 and 3, the sidewall of the test tube base 3 is formed with a first notch 302 corresponding to the rubber clamping block 12, and the sidewall of the rubber clamping sleeve 5 is formed with a second notch 502 corresponding to the rubber clamping block 12.
According to shown in figures 1 and 3, the lateral wall fixedly connected with cutting ferrule 301 of test tube base 3, the lateral wall fixedly connected with card strip 501 of rubber clamping sleeve 5, card strip 501 activity joint is in cutting ferrule 301, and the cutting ferrule 301 and the cooperation of card strip 501 that set up can have avoided rubber clamping sleeve 5 to take place spin or not hard up phenomenon in the inner chamber of test tube base 3, guarantee to carry out stable support to the test tube.
According to shown in figures 1 and 2, top center department fixedly connected with center post 2 of rotatory chassis 1, the lateral wall fixedly connected with of center post 2 strengthens post 4, strengthens post 4 and test tube base 3 fixed linking to each other, through center post 2 and the strengthening post 4 that sets up, can make and consolidate whole test tube base 3, guaranteed that when test tube base 3 high-speed rotation and carry out centrifugal operation to the test tube, test tube base 3's stability obtains guaranteeing.
According to the attached drawings 1 and 3, a round hole is formed in the sliding block 10, the sliding block 10 is movably sleeved on the fixing column 6 through the round hole, and the supporting and limiting of the sliding block 10 are guaranteed when the fixing column 6 is used for supporting and fixing the plane plate 7, so that the sliding block 10 is kept stable during movement.
According to shown in figures 3 and 4, the lateral wall fixedly connected with multiunit antislip strip 13 of the tight piece 12 of rubber clamp, the one end fixedly connected with rotation handle that slider 10 was kept away from to lead screw 9, the rotation handle person of facilitating the use who sets up rotates lead screw 9, the antislip strip 13 of setting, antislip strip 13 use the rubber material, increased the frictional force of tight piece 12 of rubber clamp and test tube outer wall for it is better to press from both sides tight fixed effect to the clamp of test tube.
The working principle is as follows: the device is when using, and the convenience is fixed the test tube, and utilize chucking fixed establishment to press from both sides tight fixedly to the test tube, make the test tube stronger with test tube base 3 and cutting ferrule 301's wholeness, when rotatory chassis 1 is high-speed rotatory, it is rotatory to drive test tube base 3 and test tube, carries out centrifugal treatment to the cell suspension in the test tube, thereby be convenient for carry out cell separation, in the processing procedure, not hard up phenomenon can not appear in the test tube, guarantees whole cell separator's stability.
Be equipped with the motor in the bottom of rotating chassis 1, can drive rotating chassis 1 after the motor starts and rotate, when the installation that carries out the test tube is fixed, at first fill in test tube base 3 with rubber clamping sleeve 5 in, then insert the inside of rubber clamping sleeve 5 with the test tube, the rotation handle on the rotatory lead screw 9 this moment can press from both sides the test tube tightly fixed.
When the lead screw 9 rotates, along 8 threaded movement of nut cover to promote the slider 10 simultaneously and slide along strengthening post 4, slider 10 drives the motion of rubber clamp tight piece 12, and finally makes the lateral wall of rubber clamp tight piece 12 and test tube offset, realizes the clamp tight to the test tube, has also formed simultaneously spacing to rubber clamp tight box 5, guarantees the stability of whole device.
When the test tube needs to be unloaded later, the reverse rotation lead screw 9 can drive the slider 10 and the rubber clamping block 12 to move reversely, and then the test tube and the rubber clamping sleeve 5 are convenient to take out.
The device is through the antislip strip 13 that sets up, and the antislip strip 13 uses the rubber material, has increased the frictional force of rubber clamp block 12 and test tube outer wall for the tight fixed effect of clamp to the test tube is better.
The clamping sleeve 301 and the clamping strip 501 are matched, so that the phenomenon that the rubber clamping sleeve 5 spins or loosens in the inner cavity of the test tube base 3 can be avoided, and the test tube is stably supported.
Through center post 2 and the reinforcing column 4 that sets up, can be so that consolidate whole test tube base 3, guaranteed that 3 high-speed rotations of test tube base and when carrying out centrifugal operation to the test tube, test tube base 3's stability obtains guaranteeing.
The foregoing shows and describes the general principles and broad features of the present invention and advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the utility model as claimed. The scope of the utility model is defined by the appended claims and equivalents thereof.

Claims (6)

1. A cell separation device for flow cytometry analysis, comprising:
the test tube test device comprises a rotating chassis (1), wherein the top of the rotating chassis (1) is fixedly connected with a plurality of groups of test tube bases (3), rubber clamping sleeves (5) are movably clamped in the test tube bases (3), and test tubes are movably clamped in the rubber clamping sleeves (5);
the clamping and fixing mechanism comprises a fixing column (6), the fixing column (6) is fixedly connected to the side wall of the test tube base (3), the end portion of the fixing column (6) is fixedly connected with a plane plate (7), a nut sleeve (8) is fixedly connected to the center of the plane plate (7), a lead screw (9) is connected to the inner thread of the nut sleeve (8), the end portion of the lead screw (9) is rotatably connected with a sliding block (10) through a bearing (11), and a rubber clamping block (12) is fixedly connected to the side wall of the sliding block (10).
2. A cell separation apparatus for flow cytometry analysis according to claim 1, wherein: the lateral wall of test tube base (3) is seted up and is pressed from both sides first notch (302) that tight piece (12) correspond with the rubber, the lateral wall of rubber clamping sleeve (5) is seted up and is pressed from both sides second notch (502) that tight piece (12) correspond with the rubber.
3. A cell separation apparatus for flow cytometry analysis according to claim 1, wherein: the lateral wall fixedly connected with cutting ferrule (301) of test tube base (3), the lateral wall fixedly connected with card strip (501) of rubber clamping sleeve (5), card strip (501) activity joint is in cutting ferrule (301).
4. A cell separation apparatus for flow cytometry analysis according to claim 1, wherein: the test tube test device is characterized in that a central column (2) is fixedly connected to the center of the top of the rotary chassis (1), a reinforcing column (4) is fixedly connected to the side wall of the central column (2), and the reinforcing column (4) is fixedly connected with the test tube base (3).
5. A cell separation apparatus for flow cytometry analysis according to claim 1, wherein: a round hole is formed in the sliding block (10), and the sliding block (10) is movably sleeved on the fixed column (6) through the round hole.
6. A cell separation apparatus for flow cytometry analysis according to claim 1, wherein: the side wall of the rubber clamping block (12) is fixedly connected with a plurality of groups of anti-slip strips (13), and one end of the screw rod (9) far away from the sliding block (10) is fixedly connected with a rotating handle.
CN202122194239.5U 2021-09-11 2021-09-11 Cell separation device for flow cytometry analysis Active CN215586802U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202122194239.5U CN215586802U (en) 2021-09-11 2021-09-11 Cell separation device for flow cytometry analysis

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202122194239.5U CN215586802U (en) 2021-09-11 2021-09-11 Cell separation device for flow cytometry analysis

Publications (1)

Publication Number Publication Date
CN215586802U true CN215586802U (en) 2022-01-21

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN202122194239.5U Active CN215586802U (en) 2021-09-11 2021-09-11 Cell separation device for flow cytometry analysis

Country Status (1)

Country Link
CN (1) CN215586802U (en)

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Date Code Title Description
GR01 Patent grant
GR01 Patent grant
PE01 Entry into force of the registration of the contract for pledge of patent right

Denomination of utility model: A cell separation device for flow cytometry analysis

Effective date of registration: 20230522

Granted publication date: 20220121

Pledgee: Overpass Branch of Jinan Rural Commercial Bank Co.,Ltd.

Pledgor: Shandong Amber Biotechnology Co.,Ltd.

Registration number: Y2023980041130

PE01 Entry into force of the registration of the contract for pledge of patent right