CN215574672U - Independent card hole digging type pesticide residue rapid detection kit - Google Patents
Independent card hole digging type pesticide residue rapid detection kit Download PDFInfo
- Publication number
- CN215574672U CN215574672U CN202121987915.8U CN202121987915U CN215574672U CN 215574672 U CN215574672 U CN 215574672U CN 202121987915 U CN202121987915 U CN 202121987915U CN 215574672 U CN215574672 U CN 215574672U
- Authority
- CN
- China
- Prior art keywords
- substrate
- developing agent
- pesticide residue
- enzyme
- color developing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000001514 detection method Methods 0.000 title claims abstract description 29
- 239000000447 pesticide residue Substances 0.000 title claims abstract description 18
- 239000000758 substrate Substances 0.000 claims abstract description 23
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 20
- 102000004190 Enzymes Human genes 0.000 claims abstract description 19
- 108090000790 Enzymes Proteins 0.000 claims abstract description 19
- 238000004806 packaging method and process Methods 0.000 claims abstract description 8
- 239000002985 plastic film Substances 0.000 claims description 5
- 229920006255 plastic film Polymers 0.000 claims description 5
- 239000004033 plastic Substances 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 3
- 229940088598 enzyme Drugs 0.000 abstract description 16
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 11
- 229940048961 cholinesterase Drugs 0.000 abstract description 6
- 238000000034 method Methods 0.000 abstract description 6
- 238000005070 sampling Methods 0.000 abstract description 6
- 102000003914 Cholinesterases Human genes 0.000 abstract description 5
- 108090000322 Cholinesterases Proteins 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 239000000203 mixture Substances 0.000 abstract description 4
- 238000004108 freeze drying Methods 0.000 description 8
- 238000012360 testing method Methods 0.000 description 6
- 239000000523 sample Substances 0.000 description 4
- 239000007853 buffer solution Substances 0.000 description 2
- 239000008176 lyophilized powder Substances 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 206010011409 Cross infection Diseases 0.000 description 1
- 206010029803 Nosocomial infection Diseases 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The utility model is suitable for the field of rapid detection, and provides an independent card hole-digging type pesticide residue rapid detection kit, which comprises a bearing piece, wherein one end of the bearing piece is provided with a storage tank for storing a substrate, and the other end of the bearing piece is provided with storage tanks for storing a color developing agent and an enzyme respectively; an enclosure mounted on the carrier, the enclosure enclosing the storage slot. Quantitative enzyme, color developing agent and substrate are respectively stored in the storage tank, and the detection personnel can disassemble the reagent at any time without proportioning the reagent, thereby saving the operation steps. In the production process, 0.1ml of enzyme, color developing agent and substrate are required to be sequentially dripped into a storage tank, and then the mixture is frozen at low temperature and covered with a packaging piece. When the device is used, the packaging piece is only required to be detached from the storage tank, one end of cholinesterase and a color developing agent is inserted into the cuvette and stirred, one end of a substrate is inserted into the cuvette and stirred, the sampling process is simple, the accuracy of a detection result is improved, and the device is very suitable for being used in a basic laboratory.
Description
Technical Field
The utility model belongs to the field of rapid detection, and particularly relates to an independent card hole-digging type pesticide residue rapid detection kit.
Background
The pesticide residue rapid detection technology is a rapid detection technology based on national standards, and when a specific detection value needs to be detected, the detection is generally carried out by adopting a spectrophotometry in the national standards. The experimental procedure was divided into control and sample tests. The testing steps are as follows: sucking 2.5ml buffer solution or sample solution with quantitative pipettor, sequentially adding 0.1ml enzyme solution and 0.1ml color developing agent, shaking, and standing in a thermostat at 37 deg.C for 15 min; adding 0.1ml of substrate after 15min, shaking up, immediately pouring into a cuvette and placing into an instrument cuvette for testing, wherein the testing time is 3 min. The detection result is positive when the inhibition rate is more than 50%, and the detection is repeated for more than 2 times for positive samples.
The rapid detection of pesticide residue has the following characteristics: firstly, the reagent sampling needs to be accurate, secondly, the reagent sampling is simple, and thirdly, the operation is simple and convenient. At present, pesticide residue rapid detection reagents on the market are generally solid powder, and three reagents need to be proportioned firstly and then used. And reagents of some manufacturers are water aqua and can be directly used. However, in both the control and sample testing processes, 0.1ml of reagent needs to be quantitatively taken, a quantitative pipette is required for sampling, and the tip of the pipette needs to be replaced during sampling to avoid cross infection.
In practice, in the detection, a non-professional inspector is generally used for detection, the use of a pipettor is not skilled, the detection reagent and the detection principle are not familiar, and the problems in the detection process cannot be analyzed and solved in time.
SUMMERY OF THE UTILITY MODEL
The embodiment of the utility model aims to provide an independent card hole digging type pesticide residue rapid detection kit, aiming at solving the problems in the background technology.
The embodiment of the utility model is realized in such a way that the kit for rapidly detecting the pesticide residue by the independent card hole digging type comprises:
the device comprises a bearing piece, a substrate, a color developing agent and an enzyme, wherein one end of the bearing piece is provided with a storage groove for storing a substrate, and the other end of the bearing piece is provided with a storage groove for storing the color developing agent and the enzyme respectively;
an enclosure mounted on the carrier, the enclosure enclosing the storage slot.
Preferably, the physical state of the substrate, developer and enzyme stored in the reservoir is a lyophilized powder.
Preferably, the carrier is a plastic card.
Preferably, the package is a plastic film.
Preferably, the storage slots at both ends of the carrier are located on the same side of the carrier.
According to the independent card hole-digging type pesticide residue rapid detection kit provided by the embodiment of the utility model, quantitative enzyme, color developing agent and substrate are respectively stored in the storage tank, and detection personnel can disassemble the kit at any time without proportioning the reagent, so that the operation steps are saved. In the production process, 0.1ml of enzyme, color developing agent and substrate are required to be sequentially dripped into a storage tank, and then the mixture is frozen at low temperature and covered with a packaging piece. Only need during the use demolish the packaging part from the holding tank, then insert the stirring in the cell with the one end of cholinesterase, colour-developing agent earlier, then insert stirring in the cell with substrate one end, can not need the pipettor completely during the sample, the sample process is simple, improves the testing result accuracy, very is fit for basic unit's laboratory and uses.
Drawings
Fig. 1 is a front view of an independent card-punched pesticide residue rapid detection kit according to an embodiment of the present invention;
fig. 2 is a side view of the independent card-punched pesticide residue rapid detection kit provided by the embodiment of the utility model.
In the drawings: 1-a substrate freeze-drying tank; 2-plastic cards; 3-a developer freeze-drying pool; 4-cholinesterase freeze-drying pool; 5-plastic film.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the utility model and are not intended to limit the utility model.
Specific implementations of the present invention are described in detail below with reference to specific embodiments.
As shown in fig. 1 and fig. 2, a structure diagram of an independent card-punched pesticide residue rapid detection kit is provided for an embodiment, and the kit includes:
the device comprises a bearing piece, a substrate, a color developing agent and an enzyme, wherein one end of the bearing piece is provided with a storage groove for storing a substrate, and the other end of the bearing piece is provided with a storage groove for storing the color developing agent and the enzyme respectively;
an enclosure mounted on the carrier, the enclosure enclosing the storage slot.
In one case of this embodiment, the storage tank stores a fixed amount of enzyme, color-developing agent and substrate, wherein the enzyme is preferably cholinesterase, and the reagent does not need to be proportioned when the detector is used, thereby saving the operation steps. In the production process, 0.1ml of enzyme, color developing agent and substrate are required to be sequentially dripped into a storage tank, and then the mixture is frozen at low temperature and covered with a packaging piece. When the device is used, the packaging piece is only required to be detached from the storage tank, one end of cholinesterase and a color developing agent is inserted into the cuvette filled with a sample solution or a buffer solution and is stirred, one end of a substrate is inserted into the cuvette and is stirred, the sampling process is simple, the accuracy of a detection result is improved, and the device is very suitable for being used in a basic laboratory.
In one case of this embodiment, the physical state of the substrate, developer and enzyme stored in the reservoir is a lyophilized powder. In the operation process, the freeze-dried powder is not easy to spill.
In one aspect of this embodiment, the storage slots at both ends of the carrier are located on the same side of the carrier. The storage tanks on the bearing piece are respectively a substrate freeze-drying tank 1, a color developing agent freeze-drying tank 3 and a cholinesterase freeze-drying tank 4 which are arranged on the same side of the bearing piece, and the packaging piece is only required to be detached once without being detached for multiple times.
As shown in fig. 1 and 2, in one embodiment, the carrier is preferably a plastic card 2, and the package is preferably a plastic film 5, which is low cost and easy to manufacture. The film 5 is torn off and used immediately after use. In the production process, 0.1ml of enzyme, color developing agent and substrate are sequentially dripped into a freeze-drying pool, and then the mixture is coated with a plastic film 5 after low-temperature freeze-drying.
It will be evident to those skilled in the art that the utility model is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the utility model being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (5)
1. An independent card hole digging type pesticide residue rapid detection kit is characterized by comprising:
the device comprises a bearing piece, a substrate, a color developing agent and an enzyme, wherein one end of the bearing piece is provided with a storage groove for storing a substrate, and the other end of the bearing piece is provided with a storage groove for storing the color developing agent and the enzyme respectively;
an enclosure mounted on the carrier, the enclosure enclosing the storage slot.
2. The kit for rapidly detecting pesticide residue by card-punch alone according to claim 1, wherein the physical state of the substrate, the color-developing agent and the enzyme stored in the storage tank is freeze-dried powder.
3. The kit for rapid detection of pesticide residue by card-punch alone of claim 1, wherein the carrier is a plastic card.
4. The kit for rapidly detecting pesticide residue by card-punch through the hole of claim 1, wherein the packaging member is a plastic film.
5. The kit for rapidly detecting pesticide residue by card-punch-out as claimed in any one of claims 1 to 4, wherein the storage slots at both ends of the carrier are located at the same side of the carrier.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121987915.8U CN215574672U (en) | 2021-08-23 | 2021-08-23 | Independent card hole digging type pesticide residue rapid detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121987915.8U CN215574672U (en) | 2021-08-23 | 2021-08-23 | Independent card hole digging type pesticide residue rapid detection kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215574672U true CN215574672U (en) | 2022-01-18 |
Family
ID=79840701
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121987915.8U Expired - Fee Related CN215574672U (en) | 2021-08-23 | 2021-08-23 | Independent card hole digging type pesticide residue rapid detection kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215574672U (en) |
-
2021
- 2021-08-23 CN CN202121987915.8U patent/CN215574672U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20220118 |