CN215560236U

CN215560236U - Fibroblast culture device

Info

Publication number: CN215560236U
Application number: CN202121084842.1U
Authority: CN
Inventors: 水庆亮; 水守燕
Original assignee: Lu'an Kangheng Biotechnology Co ltd
Current assignee: Lu'an Kangheng Biotechnology Co ltd
Priority date: 2021-05-18
Filing date: 2021-05-18
Publication date: 2022-01-18
Anticipated expiration: 2031-05-18

Abstract

The utility model discloses a fibroblast culture device, which comprises a culture tank, a culture motor, a stirrer, defoaming slurry, a cooling pipe and an air distributor, wherein the middle part of the culture tank is cylindrical, the culture motor is arranged above the top of the culture tank, the stirrer is vertically connected to a motor shaft of the culture motor, the defoaming slurry is spirally wound on the outer side of the stirrer, the air distributor is arranged at the bottom layer in the culture tank, and the stirrer vertically penetrates through the top of the culture tank and is rotatably connected to the bottom layer in the culture tank; the stirrer consists of a stirring shaft vertically connected to a motor shaft and a stirring rod horizontally connected to the stirring shaft, and the stirring rod is horizontally and symmetrically connected with defoaming slurry; the cooling pipe is formed by connecting pipes which are arranged in an inclined way end to end; one end of the air distributor is arranged on the stirring rod, and the other end of the air distributor penetrates through the side wall of the culture tank and is positioned outside the culture tank. The device drives the agitator through setting up the cultivation motor and carries out the intensive mixing to fibroblast and culture solution, accelerates fibroblast's reproduction, improves cultivation efficiency.

Description

Fibroblast culture device

Technical Field

The utility model relates to the field of cell culture, in particular to a fibroblast culture device.

Background

Fibroblasts are concerned by many people because of their great effect on repairing wounds, and how to perform fibroblast recycling and culturing is a great concern and a praise because the generation of fibroblasts can bring great benefits to people.

The in vitro culture of cells integrates biotechnology, chemical engineering and other engineering subjects, and is always a very important field for researchers. With the increasing attention of people to life science, the position of the medicine is more prominent and important. The cell culture device is used as a main carrier in the cell culture process, and provides basic material guarantee for medical and life science research at the cell level. However, most of the existing cell culture devices in the market belong to middle and low-end culture devices, and the culture efficiency is low and the culture effect is poor.

SUMMERY OF THE UTILITY MODEL

Based on the technical problems in the background art, the utility model provides a fibroblast culture device, which is provided with a stirrer to fully mix fibroblasts, so that the propagation efficiency is improved.

The utility model provides a fibroblast culture device, which comprises a culture tank, wherein the middle part of the culture tank is cylindrical, the top and the bottom of the culture tank are symmetrically arranged arc bottom types, the middle of the culture tank is cylindrical, the central axis of the culture tank is vertically arranged, the top of the culture tank is provided with a gas outlet, the fibroblast culture device also comprises a culture motor arranged above the top of the culture tank, a stirrer vertically connected to the motor shaft of the culture motor, defoaming pulp, a cooling pipe spirally wound on the outer side of the stirrer and an air distributor arranged at the bottom layer in the culture tank, and the stirrer vertically penetrates through the top of the culture tank and is rotatably connected with the bottom layer in the culture tank; the stirrer consists of a stirring shaft vertically connected to a motor shaft and a stirring rod horizontally connected to the stirring shaft, defoaming slurry is horizontally and symmetrically connected to the stirring rod, and the defoaming slurry is positioned above the stirring rod; the cooling pipe is formed by connecting obliquely arranged tubular objects end to end, is positioned below the defoaming pulp, and is connected with the outer side of the culture tank through a hose at two ends; one end of the air distributor is arranged on the stirring rod, and the other end of the air distributor penetrates through the side wall of the culture tank and is positioned outside the culture tank. The culture tank is used for providing a culture place for the fibroblasts; the gas outlet is used for flowing out gas generated by the respiration of the fibroblasts; the culture motor is used for providing power for culturing the fibroblasts; the stirrer is used for fully mixing the fibroblasts in the culture tank with the culture solution, accelerating the propagation of the fibroblasts and improving the culture efficiency of the fibroblasts; the defoaming pulp is used for defoaming the culture solution in the culture tank; the cooling pipe is used for reducing the temperature inside the culture tank, so that the condition that the temperature of culture solution inside the culture tank is increased due to the respiration and the propagation of fibroblasts is prevented, and the change of the temperature is prevented from influencing the propagation of the fibroblasts; the air distributor is used for enabling the gas entering the culture tank to be uniformly distributed in the culture tank, and the culture effect of the fibroblasts is improved.

Furthermore, the top of the stirring shaft and the top of the culture tank are aseptically sealed, a sealing sleeve is arranged on the stirring rod, the sealing sleeve is a rubber sealing sleeve, and the sealing sleeve is tightly attached to the top of the culture tank. The setting of seal cover prevents that impurity such as external air from entering into the culture tank through the clearance between the top of (mixing) shaft and culture tank inside, avoids influencing the culture of the inside fibroblast of culture tank.

Furthermore, a bearing is arranged in the center of the bottom layer in the culture tank, and the bottom of the stirring shaft is arranged on the bearing. The setting of bearing makes the puddler rotate more steadily under its supporting role to be favorable to going on smoothly of the stirring work of culture tank inside.

Further, a fibroblast culture device still includes mounting panel and brush hair, mounting panel horizontal connection is in the bottom of (mixing) shaft, the bottom parallel and level of the cylinder at mounting panel and culture tank middle part, the even and vertical connection brush hair in bottom of mounting panel, and the brush hair is not of uniform length, and the brush hair contacts with the inside bottom of culture tank. The mounting panel is used for installing the brush hair, and the brush hair is used for brushing the clearance to the inside bottom of cultivateing the jar.

Furthermore, the inner wall of the culture tank is detachably provided with a cleaning sleeve, the cleaning sleeve is tightly attached to the inner wall of the culture tank, and the height of the cleaning sleeve is consistent with the height of the middle cylinder of the culture tank. The setting of washing cover prevents that fibroblast from cultivateing and causing the pollution to the inner wall of culture tank, because washing cover demountable installation is on culture tank's inner wall, when needs wash culture tank, will wash the cover and dismantle get off wash can.

The fibroblast culture device provided by the utility model has the advantages that: according to the fibroblast culture device provided by the structure, the culture motor is arranged to drive the stirrer to fully mix the fibroblasts and the culture solution, so that the propagation of the fibroblasts is accelerated, and the culture efficiency is improved; through setting up the cooling tube, prevent that the inside temperature rise that appears because of fibroblast breathes the production of heat of culture tank, prevent that fibroblast's culture temperature is too high, further optimize fibroblast's culture environment, improve cultivation efficiency.

Drawings

FIG. 1 is a schematic structural diagram of a fibroblast culture device according to the present invention;

FIG. 2 is a cross-sectional view A-A of FIG. 1;

the device comprises a culture tank 100, a culture motor 200, a stirrer 300, a stirrer 301, a stirring shaft 302, a stirring rod 303, a sealing sleeve 400, defoaming slurry 500, a cooling pipe 600, an air distributor 700, a support bearing 800, a mounting plate 801, bristles 900 and a cleaning sleeve.

Detailed Description

As shown in fig. 1, fig. 1 is a fibroblast culture device disclosed in the present invention, wherein a stirrer is provided to fully mix fibroblasts, thereby improving the propagation efficiency.

Referring to fig. 1 and 2, the fibroblast culture device provided by the present invention comprises a culture tank 100, wherein the middle of the culture tank 100 is cylindrical, the top and the bottom of the culture tank 100 are symmetrically arranged circular arc bottom shapes, the middle of the culture tank 100 is cylindrical with a vertically arranged central axis, the top of the culture tank 100 is provided with a gas outlet 101, the fibroblast culture device further comprises a culture motor 200 arranged above the top of the culture tank 100, a stirrer 300 vertically connected to a motor shaft of the culture motor 200, defoaming slurry 400, a cooling pipe 500 spirally wound on the outer side of the stirrer 300, and an air distributor 600 arranged at the bottom layer inside the culture tank 100, wherein the stirrer 300 vertically penetrates through the top of the culture tank 100 and is rotatably connected to the bottom layer inside the culture tank 100; the stirrer 300 is composed of a stirring shaft 301 vertically connected to a motor shaft and a stirring rod 302 horizontally connected to the stirring shaft 301, defoaming paddles 400 are horizontally and symmetrically connected to the stirring rod 302, and the defoaming paddles 400 are positioned above the stirring rod 302; the cooling pipe 500 is formed by connecting obliquely arranged pipes end to end, the cooling pipe 500 is positioned below the defoaming agent 400, and two ends of the cooling pipe 500 are connected with the outer side of the culture tank 100 through hoses; one end of the air distributor 600 is mounted on the stirring bar 302, and the other end of the air distributor 600 passes through the sidewall of the culture tank 100 and is located outside the culture tank 100. Adding culture solution and fibroblasts into a culture tank 100, installing a culture motor 200, a stirrer 300, defoaming slurry 400, a cooling pipe 500 and an air distributor 600 on the culture tank 100, starting the culture motor 200, driving the stirrer 300 and the defoaming slurry 400 to rotate by the culture motor 200, fully mixing and stirring the fibroblasts and the culture solution while rotating the stirrer 300, so that the fibroblasts are better propagated, gas generated during propagation of the fibroblasts escapes from a gas outlet 101, and foams on the culture solution in the culture tank 100 are eliminated while the defoaming slurry 400 rotates, so that the breathing and propagation of the fibroblasts are prevented from being influenced; let in cold water to cooling tube 500, cold water enters into culture tank 100 inside and circulates in the cooling tube 500, prevents that culture tank 100 is inside to produce the temperature rise because of fibroblast breathes, guarantees fibroblast's culture temperature, and then is favorable to fibroblast's cultivation.

Referring to fig. 1, the stirring shaft 301 and the top of the culture tank 100 are aseptically sealed, a sealing sleeve 303 is installed on the stirring rod 302, the sealing sleeve 303 is a sealing sleeve 303, and the sealing sleeve 303 is tightly attached to the top of the culture tank 100. The annular sealing sleeve 303 made of rubber is sleeved on the top of the stirring shaft 301, and then the sealing sleeve 303 is slowly moved downwards along the stirring shaft 301 until the sealing sleeve 303 is tightly attached to the outer side of the top of the culture tank 100.

Referring to fig. 1, a bearing 700 is installed at the center of the inner bottom layer of the culture tank 100, and the bottom of the stirring shaft 301 is installed on the bearing 700. The cultivation motor 200 is started, the cultivation motor 200 drives the top end of the stirring shaft 301 to rotate, and the bottom of the stirring shaft 301 is installed on the bearing 700, so that the whole stirring shaft 301 can stably rotate inside the cultivation hanger under the supporting effect of the bearing 700.

Referring to fig. 1, the fibroblast culture device further comprises a mounting plate 800 and bristles 801, wherein the mounting plate 800 is horizontally connected to the bottom of the stirring shaft 301, the mounting plate 800 is flush with the cylindrical bottom of the middle part of the culture tank 100, the bristles 801 are uniformly and vertically connected to the bottom of the mounting plate 800, the bristles 801 are different in length, and the bristles 801 are in contact with the bottom layer inside the culture tank 100. The culture motor 200 drives the stirring shaft 301 to rotate, the stirring shaft 301 drives the bristles 801 to rotate, and the bristles 801 brush the bottom layer inside the culture tank 100 when rotating.

Referring to fig. 1, the inner wall of the culture tank 100 is detachably provided with a cleaning sleeve 900, the cleaning sleeve 900 is tightly attached to the inner wall of the culture tank 100, and the height of the cleaning sleeve 900 is consistent with the height of the middle cylinder of the culture tank 100. After the fibroblast culture work is finished, the cleaning sleeve 900 is detached and cleaned, and the cleaning sleeve is placed in the culture tank 100 to be tightly attached to the inner wall of the culture tank 100 after being cleaned and aired, so that preparation is made for next cell culture.

The working process is as follows: adding culture solution and fibroblasts into the culture tank 100, installing the culture motor 200, the stirrer 300, the defoaming slurry 400, the cooling pipe 500 and the air distributor 600 on the culture tank 100, sleeving a circular sealing sleeve made of rubber on the top of the stirring shaft 302, and then slowly moving the sealing sleeve downwards along the stirring shaft 302 until the sealing sleeve is tightly attached to the outer side of the top of the culture tank 100.

Open and cultivate motor 200, it drives the top rotation of (mixing) shaft 302 to cultivate motor 200, because the bottom of (mixing) shaft 302 is installed on bearing 700, under bearing 700's supporting role, whole (mixing) shaft 302 is hanging inside steadily rotating in the cultivation, (mixing) shaft 302 drives puddler 303 and defoaming thick liquid 400 and rotates, puddler 303 carries out abundant mixture and stirring to fibroblast and culture solution when pivoted, make fibroblast breed better, produced gas escapes from gas outlet 101 during the fibroblast breeds, defoaming thick liquid 400 is eliminated the foam on the culture solution of culture tank 100 inside in the pivoted, prevent that it from having influenced fibroblast's breathing and reproduction.

Let in cold water to cooling tube 500, cold water enters into culture tank 100 inside and circulates in the cooling tube 500, prevents that culture tank 100 is inside to produce the temperature rise because of fibroblast breathes, guarantees fibroblast's culture temperature, and then is favorable to fibroblast's cultivation.

After the fibroblast culture work is finished, the cleaning sleeve 900 is detached and cleaned, and the cleaning sleeve is placed in the culture tank 100 to be tightly attached to the inner wall of the culture tank 100 after being cleaned and aired, so that preparation is made for next cell culture.

In summary, the fibroblast culture device provided by the structure of the utility model has the advantages that the culture motor is arranged to drive the stirrer to fully mix the fibroblasts and the culture solution, so that the propagation of the fibroblasts is accelerated, and the culture efficiency is improved; through setting up the cooling tube, prevent that the inside temperature rise that appears because of fibroblast breathes the production of heat of culture tank, prevent that fibroblast's culture temperature is too high, further optimize fibroblast's culture environment, improve cultivation efficiency.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be able to cover the technical scope of the present invention by equivalent replacement or change according to the technical solution and the inventive concept of the present invention within the technical scope of the present invention.

Claims

1. A fibroblast culture device comprises a culture tank (100), wherein the middle of the culture tank (100) is cylindrical, the top and the bottom of the culture tank (100) are arc bottom-shaped and symmetrically arranged, the middle of the culture tank (100) is cylindrical, a central axis of the center of the; the stirrer (300) consists of a stirring shaft (301) vertically connected to a motor shaft and a stirring rod (302) horizontally connected to the stirring shaft (301), defoaming slurry (400) is horizontally and symmetrically connected to the stirring rod (302), and the defoaming slurry (400) is positioned above the stirring rod (302); the cooling pipe (500) is formed by connecting obliquely arranged tubular objects end to end, the cooling pipe (500) is positioned below the defoaming pulp (400), and two ends of the cooling pipe (500) are connected with the outer side of the culture tank (100) through hoses; one end of the air distributor (600) is arranged on the stirring rod (302), and the other end of the air distributor (600) penetrates through the side wall of the culture tank (100) and is positioned outside the culture tank (100).

2. The fibroblast culture device according to claim 1, wherein the stirring shaft (301) is aseptically sealed with the top of the culture tank (100), a sealing sleeve (303) is mounted on the stirring rod (302), the sealing sleeve (303) is a rubber sealing sleeve, and the sealing sleeve (303) is tightly attached to the top of the culture tank (100).

3. Fibroblast cell culture apparatus, according to claim 1, wherein the bottom layer of the inside of the culture tank (100) is centrally mounted with a bearing (700), and the bottom of the stirring shaft (301) is mounted on the bearing (700).

4. The fibroblast culture device according to claim 1, further comprising a mounting plate (800) and bristles (801), wherein the mounting plate (800) is horizontally connected to the bottom of the stirring shaft (301), the mounting plate (800) is flush with the cylindrical bottom of the middle part of the culture tank (100), the bottom of the mounting plate (800) is uniformly and vertically connected with the bristles (801), the bristles (801) are different in length, and the bristles (801) are in contact with the inner bottom layer of the culture tank (100).

5. The fibroblast culture device according to claim 1, wherein the inner wall of the culture tank (100) is detachably provided with a cleaning sleeve (900), the cleaning sleeve (900) is tightly attached to the inner wall of the culture tank (100), and the height of the cleaning sleeve (900) is consistent with the height of the cylinder in the middle of the culture tank (100).